CN206033759U - Among ship ballast water 10 biological quick detection device of 50um - Google Patents
Among ship ballast water 10 biological quick detection device of 50um Download PDFInfo
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- CN206033759U CN206033759U CN201621004238.2U CN201621004238U CN206033759U CN 206033759 U CN206033759 U CN 206033759U CN 201621004238 U CN201621004238 U CN 201621004238U CN 206033759 U CN206033759 U CN 206033759U
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- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
The utility model provides an among ship ballast water 10 biological quick detection device of 50um, relate to ship ballast water check out test set, including sample box, the flush fluid case, the lysate case, the waste water tank, the detection case, level filtering ware and second grade filter, the export of level filtering ware links to each other with the sample box import, be equipped with coloring agent inlet port on the detection case, sample box, be equipped with the sample pump in the export of flush fluid case and coloring agent case respectively, washer pump and schizolysis pump, sample pump strainer valve links to each other with secondary filter, secondary filter links to each other with the washer pump through the flushometer, connecting pipe between secondary filter and flushometer links to each other with the waste water tank through the flowing back valve, the connecting line of inlet through between feed liquor valve and strainer valve and secondary filter of detection case links to each other, the schizolysis is pumped the mouth and is linked to each other with the detection case, be equipped with the photofluorometer on the detection case. Simple structure, convenient to use have, detect fast, the precision is high, data advantage such as true and reliable.
Description
Technical field
The present invention relates to ship carry ballast water in biological living testing equipment, say it is a kind of accuracy of detection in detail
10-50um biologies device for fast detecting in the true and reliable ballast water for ship of high, data.
Background technology
It is known that transport by sea ship is in order to ensure stability of ship, typically ballast water is installed additional originating harbour, wait to stop
Discharge ballast water when leaning against purpose harbour again, the biology entrained by which can bring biotic intrusion risk to purpose Port State.In order to
Tackle this problem, International Maritime Organization（IMO）, United States Coasts Guard（USCG）And Environmental Protection Agency（EPA）Formulate
Corresponding biotic intrusion counter-measure and discharge of ballast water control pact, it is desirable to which most commercial transportation ships must install ballast additional
Water treatment facilities（BWTS）To process ballast water.IMO G8 directive/guides and the ETV draft clear stipulaties discharge ballast of USCG references
The size and viable biological concentration of organism in water, it is as follows：
Minimum dimension is less than 10/m more than or equal to the viable biological of 50 m3；
Minimum dimension is less than 50 m but the viable biological more than or equal to 10 m is less than 10/ml；
And used as health standard, target microorganism is less than following concentration：
Poisonous comma bacillus (serotype O1 and O139) is less than 1 cfu/100ml；
Escherichia coli：Less than 250 cfu/100ml；
Enterococcus：Less than 100 cfu/100ml.
At present, IMO worlds compressive effect also Pending The Entry Into Force, but effective date also closes on.USCG is unilaterally announced
Its specification comes into force, and the commercial transportation ship of all entrance U.S. waterses of mandatory provision must be installed and meet USCG specifications and discharge standard
BWTS, cannot otherwise reach port carries out related operation.Because of the water quality and bio distribution situation difference in each waters, ship institute
The BWTS of installation is it cannot be guaranteed that the ballast water of discharge can meet discharge standard every time, it is necessary to before near Port State discharge voluntarily
Detection target organism content, therefore, on ship, testing staff must have abundant biological basis knowledge and detection experience, while matching somebody with somebody
Standby advanced biological detection instrument.For most ship's repairs ＆ maintenance facilities and crewman's know-how, it is difficult to meet this requirement.
In general, 10-50um is biological is mainly unicellular alga, because of its species particularity, can neither pass through filtration side
Method is removed completely, can not pass through ultraviolet irradiation or chemical method is killed completely, and species is various, it has also become ballast organism in water
The difficult point of detection.Existing device for fast detecting is to estimate frustule quantity according to the chlorophyllous content of algae, be our experiments show that,
Chlorophyll in the dead frustule of part still can keep complete, according to the method that chlorophyll content judges cell survival conditions
Error is big, reliability is low, or even erroneous judgement occurs.On the other hand, existing testing equipment can will be the algae of some below 10um thin
Born of the same parents also count, and detection data is not accurate.
The content of the invention
Present invention aim to address above-mentioned the deficiencies in the prior art, there is provided a kind of simple structure, easy to use, detection speed
Degree is fast, high precision, 10-50um biologies device for fast detecting in the true and reliable ballast water for ship of data.
The present invention solves the technical scheme adopted by above-mentioned the deficiencies in the prior art：
10-50um biologies method for quick in a kind of ballast water for ship, it is characterised in that comprise the steps：
(1) using large aperture filter by Jing after the process of ballast water treatment equipment（To be discharged）In ballast water sample not
Viable biological less than 50um is leached, and obtains filter liquor；
（2）Aml filter liquors are taken, is filtered and is retained the survival in filter liquor not less than 10um using small-bore filter and give birth to
Thing, the viable biological less than 10um are leached with liquid；
（3）Using 3-10%Aml cleaning fluid backwash small-bore filter, by retention size 10-50um survival
Biology is flushed in sample cell；
（4）Live cell fluorescent coloring agent is added into sample cell, dyes 5-30（10）Minute；
（5）After dyeing, the cell pyrolysis liquid of which in liquid volume 0.5-2 times is added into sample cell；
（6）Treat cell（Film）Fully split（It is molten）Xie Hou, using the relative intensity of fluorescence of liquid in fluorescence photometer detection sample groove；
The acquisition methods of the strong reference value of relative fluorescence are as follows：
A, from known symbols close ballast water treatment D-2 standard in deposit to the regulation of 10-50um viable biologicals, containing 10-50um
Living organism number reaches the water sample of the upper limit as detection sample is referred to, will be with reference to not little in detection sample using large aperture filter
Leach in the viable biological of 50um, obtain filter liquor；
B, take Aml filter liquors, filtered using small-bore filter and retained viable biological in filter liquor not less than 10um,
Viable biological less than 10um is leached with liquid；
C, using 3-10%Aml cleaning fluid backwash small-bore filter, by retention size 10-50um survival
Biology is flushed in sample cell；
D, into sample cell add live cell fluorescent coloring agent, dye 5-30（10）Minute；
After e, dyeing, the cell pyrolysis liquid of liquid volume 0.5-2 times in which is added into sample cell；
F, after cell is fully cracked, using the relative intensity of fluorescence of liquid in fluorescence photometer detection sample groove；
G, the different reference detection sample of 1-100 kinds is chosen by the selection standard in step a with reference to detection sample, per seed ginseng
Examine detection sample to detect at least one times by the method for operating of step a, b, c, d, e, f, average relative is obtained according to the data obtained glimmering
Light intensity value, the average relative fluorescence intensity level are relative intensity of fluorescence reference value；
By（6）The relative intensity of fluorescence value that step is obtained walks the relative intensity of fluorescence reference value contrast for obtaining with g, judges
Whether the ballast water sample meets discharge standard.
The following method of reference detection sample Jing in the present invention described in a steps is obtained：Using large aperture filter by Jing
After the process of ballast water treatment equipment（To be discharged）Viable biological in ballast water sample not less than 50um is leached, and must be leached
Liquid；Aml filter liquors are taken, filtered and is retained the viable biological in filter liquor not less than 10um using small-bore filter, be less than
The viable biological of 10um is leached with liquid；Using the cleaning fluid backwash small-bore filter of 3-10%Aml, the size that will be retained
It is flushed in sample cell in the viable biological of 10-50um；By the liquid Jing FDA-CMFDA methods in sample cell in fluorescence microscopy
Detect under mirror, when the content of wherein 10-50um viable biologicals meets the requirements, you can become and refer to detection sample.
Reference detection sample in the present invention described in a steps also can the following method acquisitions of Jing：It is artificial to prepare algae solution
Or nature seawater Jing ultraviolets（UV）Process or Jing sodium hypochlorite（NaClO）Process, obtain test fluid；Will using large aperture filter
Viable biological in test fluid not less than 50um is leached, and obtains filter liquor；Aml filter liquors are taken, is filtered simultaneously using small-bore filter
Viable biological, the viable biological less than 10um in retention filter liquor not less than 10um is leached with liquid；Using 3-10%Aml's
Cleaning fluid backwash small-bore filter, the viable biological by the size of retention in 10-50um are flushed in sample cell；Will
Liquid Jing FDA-CMFDA methods in sample cell detect that under fluorescence microscope the content of wherein 10-50um viable biologicals is conformed to
When asking, you can become and refer to detection sample.
Heretofore described large aperture filter is by being made up of the nylon bolting silk of 35-50um using mesh catercorner length
Filter；Described small-bore filter be using absolute hole diameter for 10um miillpore filter made by, can backwash
Filter；
Heretofore described live cell fluorescent coloring agent is FDA or/and CMFDA, and its addition is according to liquid in sample cell
Volume, with 1-3mg/ml（2mg/ml）Ratio add.The staining reaction time is 5-30（10）Minute.FDA is fluorescein diethyl
Acid esters.
Heretofore described cleaning fluid is BES, MOPS, PBS（pH=7.0）, Tris-HCl (0.2-1.0M, pH=6.0-
7.2) at least one in.The abiotic hydrolysis of FDA or CMFDA can effectively be prevented.
Heretofore described cell pyrolysis liquid is methyl alcohol and chloroform（Volume ratio 1:1-1:3）Mixed liquor or acetone in one
Kind.
10-50um biologies device for fast detecting in a kind of ballast water for ship, it is characterised in that including sales kit (SK), flushing liquor
Case, cracking liquid case, waste water tank, detection case, grade one filter and two grades of filters, outlet and the sales kit (SK) import phase of grade one filter
Even, detection case is provided with coloring agent and adds mouth, sales kit (SK), rinse be respectively equipped with the outlet of liquid case and coloring agent case sample pump,
Flushing pump and cracking pump, the outlet of sample pump is connected with the Single port of secondary filter through trap valve, secondary filter it is another
Port Jing flushing valves are connected with pump discharge is rinsed, the connecting tube Jing tapping valve between secondary filter and flushing valve and waste water tank phase
Even, the inlet Jing liquid feed valves of detection case are connected with the connecting line between strainer valve and secondary filter, crack pump discharge and inspection
Measuring tank is connected, and detection case is provided with fluorescence photometer.
Heretofore described grade one filter is the nylon bolting silk that mesh catercorner length is 35-50um；Two grades of filters
For the miillpore filter that absolute hole diameter is 10um；The wavelength of transmitted light of fluorescence photometer is 460-490nm, and the excitation light wave of reception is a length of
510-550nm.Described detection lower box part Jing drain valves are connected with waste water tank.
Heretofore described 10-50um biologies are referred to：It is smaller in size than 50um but the viable biological more than or equal to 10um.
The 10-50um biochrons in ballast water for ship to be discharged are detected using the device and method of the present invention, is pressed first
According to the strong reference value of relative fluorescence acquisition methods testing out using specific certain cleaning fluid, live cell fluorescent coloring agent, thin
Relative intensity of fluorescence reference value when cellular lysate liquid is combined；When ship needs detection, using specific cleaning fluid, the living cells
Fluorescent dye, the combination of cell pyrolysis liquid detect relative intensity of fluorescence according to step 1-6, when relative intensity of fluorescence is not more than
During relative intensity of fluorescence reference value, illustrate that ballast water for ship to be discharged meets discharge standard, otherwise illustrate ship to be discharged
Ballast water does not meet discharge standard, and in the case of identical various reagents, relative intensity of fluorescence reference value only needs inspection one
It is secondary, you can repeatedly, even check many times in become reference value, in the case of having this reference value to obtain, present configuration is simple, make
With conveniently, detection speed is fast, high precision, data are true and reliable.
Description of the drawings
Fig. 1 is the structural representation of speed detector in the present invention.
Fig. 2 is the corresponding relation figure in the present invention between relative intensity of fluorescence and four slit bamboo or chopped wood algae living cells contents.
Specific embodiment
In a kind of ballast water for ship, 10-50um biologies method for quick, comprises the steps：
(1) will be not little in the ballast water sample to be discharged Jing after the process of ballast water treatment equipment using large aperture filter
Leach in the viable biological of 50um, obtain filter liquor；
（2）Aml filter liquors are taken, is filtered and is retained the survival in filter liquor not less than 10um using small-bore filter and give birth to
Thing, the viable biological less than 10um are leached with liquid；
（3）Using 3-10%Aml cleaning fluid backwash small-bore filter, by retention size 10-50um survival
Biology is flushed in sample cell；
（4）Live cell fluorescent coloring agent is added into sample cell, dyes 5-30 minutes；
（5）After dyeing, the cell pyrolysis liquid of which in liquid volume 0.5-2 times is added into sample cell；
（6）After cell is fully cracked, using the relative intensity of fluorescence of liquid in fluorescence photometer detection sample groove；
The acquisition methods of the strong reference value of relative fluorescence are as follows：
A, from known symbols close ballast water treatment D-2 standard in deposit to the regulation of 10-50um viable biologicals, containing 10-50um
Living organism number reaches the water sample of the upper limit as referring to detection sample；Will be with reference to not little in detection sample using large aperture filter
Leach in the viable biological of 50um, obtain filter liquor；
B, take Aml filter liquors, filtered using small-bore filter and retained viable biological in filter liquor not less than 10um,
Viable biological less than 10um is flowed out with liquid；
C, using 3-10%Aml cleaning fluid backwash small-bore filter, by retention size 10-50um survival
Biology is flushed in sample cell；
D, into sample cell add live cell fluorescent coloring agent, dye 5-30（10）Minute；
After e, dyeing, the cell pyrolysis liquid of liquid volume 0.5-2 times in which is added into sample cell；
F, treat cell（Film）Fully split（It is molten）Xie Hou, using the relative intensity of fluorescence of liquid in fluorescence photometer detection sample groove；
G, the different reference detection sample of 1-100 kinds is chosen by the selection standard in step a with reference to detection sample, per seed ginseng
Examine detection sample to detect at least one times by the method for operating of step a, b, c, d, e, f, average relative is obtained according to the data obtained glimmering
Light intensity value, the average relative fluorescence intensity level are relative intensity of fluorescence reference value；
By（6）The relative intensity of fluorescence value that step is obtained walks the relative intensity of fluorescence reference value contrast for obtaining with g, judges
Whether the ballast water sample meets discharge standard.
Wherein A is any numerical value, the preferably natural number of 100-500.
The following method of reference detection sample Jing in the present invention described in a steps is obtained：Using large aperture filter by Jing
Viable biological in ballast water sample to be discharged after the process of ballast water treatment equipment not less than 50um is leached, and obtains filter liquor；
Take Aml filter liquors, filtered using small-bore filter and retained in filter liquor not less than 10um viable biological, less than 10um's
Viable biological is leached with liquid；Using 3-10%Aml cleaning fluid backwash small-bore filter, by retention size in 10-
The viable biological of 50um is flushed in sample cell；By the liquid Jing FDA-CMFDA methods in sample cell under fluorescence microscope
Detection, when the content of wherein 10-50um viable biologicals meets the requirements, the pressure to be discharged Jing after the process of ballast water treatment equipment
Become by carrying water sample and refer to detection sample.
Reference detection sample in the present invention described in a steps also can the following method acquisitions of Jing：It is artificial to prepare algae solution
Or nature seawater Jing ultraviolets（UV）Process or Jing sodium hypochlorite（NaClO）Process, obtain test fluid；Will using large aperture filter
Viable biological in test fluid not less than 50um is leached, and obtains filter liquor；Aml filter liquors are taken, is filtered simultaneously using small-bore filter
Viable biological, the viable biological less than 10um in retention filter liquor not less than 10um is leached with liquid；Using 3-10%Aml's
Cleaning fluid backwash small-bore filter, the viable biological by the size of retention in 10-50um are flushed in sample cell；Will
Liquid Jing FDA-CMFDA methods in sample cell detect that under fluorescence microscope the content of wherein 10-50um viable biologicals is conformed to
When asking, become by the ballast water sample to be discharged Jing after the process of ballast water treatment equipment and refer to detection sample.
Further, described large aperture filter is the nylon bolting silk using mesh catercorner length for 35-50um
Made by filter；Described small-bore filter be using absolute hole diameter for 10um miillpore filter made by, can backwash
Filter.The live cell fluorescent coloring agent is FDA or/and CMFDA, its addition according to liquid volume in sample cell, with
1-3mg/ml（2mg/ml）Ratio add.The staining reaction time is 5-30（10）Minute.FDA is fluorescein(e) diacetate；Institute
The cleaning fluid stated is BES, MOPS, PBS（pH=7.0）, at least one in Tris-HCl (0.2-1.0M, pH=6.0-7.2), can
The abiotic hydrolysis of FDA or CMFDA is prevented effectively.Wherein PBS is：Vulcanized lead, Tris-HCl are：Three（Methylol）Amino first
Alkane.Described cell pyrolysis liquid is methyl alcohol and chloroform（Volume ratio 1:1-1:3）Mixed liquor or acetone in one kind.
10-50um biologies device for fast detecting in ballast water for ship as shown in Figure 1, including sales kit (SK) 2, rinses liquid case
13rd, liquid case 3, waste water tank 14, detection case 17, grade one filter 1 and two grades of filters 8 are cracked, grade one filter 1 is using mesh pair
Diagonal length filter made by the nylon bolting silk of 50um；Two grades of filters are using the miillpore filter system that absolute hole diameter is 10um
Into filter.The outlet of grade one filter is connected with 2 import of sales kit (SK), and the liquid Jing after grade one filter is filtered is entered and sample
Primary pump can be provided with connecting line between grade one filter and sales kit (SK) 2 in product case 2, or grade one filter is located at sample
Above product case 2, flowed into using the gravity of liquid naturally.Detection case 17 is provided with coloring agent and adds mouth 12, sales kit (SK) 2, flushing liquor
Sample pump 4, flushing pump 13 and cracking pump 5 is respectively equipped with the outlet of case 14 and cracking liquid case 3, and the outlet Jing of sample pump 4 is filtered
Valve 6 is connected with the Single port of secondary filter 8, and another port Jing flushing valve 11 and the flushing pump 13 of secondary filter 8 export phase
Even, the connecting tube Jing tapping valve 10 between secondary filter 8 and flushing valve 11 is connected with waste water tank 15, the inlet Jing of detection case 17
Connecting line between liquid feed valve 9 and strainer valve 6 and secondary filter 8 is connected, and the cracking outlet Jing of pump 5 cracks valve 7 and detection case 17
It is connected, detection case 17 is provided with fluorescence photometer 18, and the detection probe of fluorescence photometer 18 is located in detection case 17；The transmitting light wave of fluorescence photometer
A length of 460-490nm, a length of 510-550nm of excitation light wave of reception；Described detection lower box part Jing drain valves 16 and waste water tank
15 are connected.
Heretofore described 10-50um biologies are referred to：It is smaller in size than 50um but the viable biological more than or equal to 10um.
When 10-50um biologies device for fast detecting works in ballast water for ship, ballast water sample to be detected is put into into one
In level filter, the viable biological in ballast water sample not less than 50um is leached by grade one filter, leaching after being filtered
Liquid is entered in sales kit (SK), is separately added into cleaning fluid and cell pyrolysis liquid to rinsing in liquid case and cracking liquid case；Open strainer valve and
Filter liquor Jing secondary filter in sales kit (SK) is filtered by tapping valve, other valve closings, sample pump work, quantitative, is leached
Liquid enter in waste water tank, and be not less than the viable biological of 10um and retained by secondary filter；Strainer valve and tapping valve is closed,
Now dye valve and drain valve is also at closed mode, open flushing valve and liquid feed valve, rinse pump work, using quantitative flushing
Liquid is backwashed to secondary filter, and the 10-50um biologies for being retained are flushed in detection case；Closing flushing valve and liquid feed valve,
Warp-wise coloring agent adds mouth to add appropriate live cell fluorescent coloring agent into sales kit (SK), carry out fluorescent staining to living cells；It is right
Living cells dyeing is completed（About 10 minutes）Afterwards, cracking valve is opened, pump work is cracked, cell pyrolysis liquid is input in detection case, is treated
After cell is fully cracked, the relative intensity of fluorescence value in detection case is measured using fluorescence.Choose 80 kinds and pass through laboratory method
Ballast water water sample to be discharged have determined, containing 9 10-50um viable biologicals passes through as ballast water sample to be detected
Above-mentioned using method is detected that using the device averaged is used as relative intensity of fluorescence reference value；By relative fluorescence
Intensity level is contrasted with relative intensity of fluorescence reference value, when relative intensity of fluorescence is not more than relative intensity of fluorescence reference value, explanation
Ballast water for ship to be discharged meets discharge standard, otherwise illustrates that ballast water for ship to be discharged does not meet discharge standard, uses
In the case of identical various reagents, relative intensity of fluorescence reference value only demand takes once, you can repeatedly, even examining many times
Become reference value in testing, in the case of having this reference value, present configuration is simple, easy to use, detection speed is fast, high precision,
Data are true and reliable.
Example 1
In a kind of ballast water for ship, 10-50um biologies method for quick, comprises the steps：Using mesh diagonal line length
Made by the nylon bolting silk of 50um filter is spent by the ballast water sample to be discharged Jing after the process of ballast water treatment equipment
Viable biological not less than 50um is leached, and obtains filter liquor；200ml filter liquors are taken, the viable biological less than 10um is leached with liquid；
Small-bore filter, the chi that will be retained are backwashed using Tris-HCl of the substance withdrawl syndrome of 10ml for 0.8mol/L, pH=7
The very little viable biological in 10-50um is flushed in sample cell；Live cell fluorescent coloring agent fluorescein is added into sample cell
Diacetate esters 20mg, dyes 10 minutes；After the completion of dyeing, the cell pyrolysis liquid of 10ml is added into sample cell（Cell pyrolysis liquid
For methyl alcohol and chloroform by volume 1:2 liquid for mixing）, stand 5 minutes；After cell is fully cracked, examined using fluorescence photometer
Survey the relative intensity of fluorescence of liquid in sample cell.
The acquisition methods of the strong reference value of relative fluorescence are as follows：From known to 60 kinds, per milliliter containing 9 10-50um survival
Biological ballast water water sample to be discharged is used as referring to detection sample；Every kind of reference detection sample is proceeded as follows：Using net
Hole catercorner length for 50um nylon bolting silk made by filter by with reference in detection sample not less than 50um viable biological
Leach, obtain filter liquor；200ml filter liquors are taken, the viable biological less than 10um is leached with liquid；Amount using the material of 10ml is dense
Spend for 0.8mol/L, pH=7 Tris-HCl backwash small-bore filter, by retention size 10-50um viable biological
It is flushed in sample cell；Live cell fluorescent coloring agent fluorescein(e) diacetate 20mg is added into sample cell, dyes 10 points
Clock；After the completion of dyeing, the cell pyrolysis liquid of 10ml is added into sample cell（Cell pyrolysis liquid is methyl alcohol and chloroform by volume 1:
2 liquid for mixing）, stand 5 minutes；After cell is fully cracked, using in fluorescence photometer detection sample groove liquid it is relatively glimmering
Light intensity value.This 60 kinds mean values with reference to the relative intensity of fluorescence obtained after detection sample process are calculated, the mean value is
Relative intensity of fluorescence reference value.The relative intensity of fluorescence value obtained after ballast water treatment to be detected is referred to relative intensity of fluorescence
Value contrast, you can judge whether the ballast water sample to be detected meets discharge standard.
Obtain with reference to the following method of detection sample Jing：Using large aperture filter by Jing after the process of ballast water treatment equipment
Viable biological in ballast water sample to be discharged not less than 50um is leached, and obtains filter liquor；Aml filter liquors are taken, using small-bore
Filter is filtered and is not less than the viable biological of 10um, the viable biological less than 10um in retaining filter liquor and leaches with liquid；Make
It is flushed into the cleaning fluid backwash small-bore filter of 3-10%Aml, the viable biological by the size of retention in 10-50um
To in sample cell；Liquid Jing FDA-CMFDA methods in sample cell are detected under fluorescence microscope, wherein 10-50um survival lifes
When the content of thing meets the requirements, you can become and refer to detection sample.Be respectively adopted in the present invention cleaning fluid from BES, MOPS,
PBS（pH=7.0）, at least one in Tris-HCl (0.2-1.0M, pH=6.0-7.2), can reduce FDA's to greatest extent
Abiotic hydrolysis.Cell pyrolysis liquid leaches can the fluorescein inside living cells, improve the accuracy of testing result.
FDA is connected with the acetic acid free radical of two conjugation, is a kind of apolar substance, can pass freely through alga cells film, quilt
The non-specific enzymatic hydrolysis such as esterase, protease, lipase in cell are into fluorescein.FDA is leuco-compounds, and itself does not have
Have fluorescence, and product fluorescein be a kind of polarity and the material with fluorescence radiation performance, stable chemical nature, be difficult by
Decompose, it is difficult to through cell membrane, accumulate in the cell.When intracellular storage fluorescein exceed it is a certain amount of after be discharged into environment
In, due to reactant it is different from the fluorescent characteristic of product, even if therefore reactant is superfluous, the also not measure of interference product.
Relation between relative intensity of fluorescence and 10-50um biology contents
Four slit bamboo or chopped wood algae solution of variable concentrations are prepared, its survivaling cell content is determined using the detection of FDA-CMFDA methods.Will be each molten
Liquid is added in the device of the present invention, carries out the test of relative intensity of fluorescence using the method for the present invention.With four slit bamboo or chopped wood algae living cells
Content is x-axis, with the relative intensity of fluorescence that measures as y-axis, makes Fig. 2, as shown in Figure 2, the testing result and reality of the present invention
Living cells content has preferable one-to-one relationship, can reflect the living cells content in sample.
Claims (2)
1. in a kind of ballast water for ship 10-50um biology device for fast detecting, it is characterised in that：Including sales kit (SK), rinse liquid case,
Cracking liquid case, waste water tank, detection case, grade one filter and two grades of filters, the outlet of grade one filter are connected with sales kit (SK) import,
Detection case is provided with coloring agent and adds mouth, is respectively equipped with sample pump, flushing in the outlet of sales kit (SK), flushing liquid case and coloring agent case
Pump and cracking pump, the outlet of sample pump are connected with the Single port of secondary filter through trap valve, the another port of secondary filter
Jing flushing valves are connected with pump discharge is rinsed, and the connecting tube Jing tapping valve between secondary filter and flushing valve is connected with waste water tank, inspection
The inlet Jing liquid feed valves of measuring tank are connected with the connecting line between strainer valve and secondary filter, crack pump discharge and detection case phase
Even, detection case is provided with fluorescence photometer.
2. in ballast water for ship according to claim 1 10-50um biology device for fast detecting, it is characterised in that：It is described
Grade one filter be mesh catercorner length for 35-50um nylon bolting silk；Two grades of filters are that absolute hole diameter is the micro- of 10um
Hole filter membrane；The wavelength of transmitted light of fluorescence photometer is 460-490nm, a length of 510-550nm of excitation light wave of reception.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106244665A (en) * | 2016-08-31 | 2016-12-21 | 威海中远造船科技有限公司 | Method and device for quickly detecting 10-50um organisms in ship ballast water |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106244665A (en) * | 2016-08-31 | 2016-12-21 | 威海中远造船科技有限公司 | Method and device for quickly detecting 10-50um organisms in ship ballast water |
| WO2018041215A1 (en) * | 2016-08-31 | 2018-03-08 | 威海中远造船科技有限公司 | Fast detection method and device for organisms of 10-50 μm in ship ballast water |
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