CN1975380A - Reagent formula of optical detection for assaying human body hemoglobin - Google Patents
Reagent formula of optical detection for assaying human body hemoglobin Download PDFInfo
- Publication number
- CN1975380A CN1975380A CN 200510126008 CN200510126008A CN1975380A CN 1975380 A CN1975380 A CN 1975380A CN 200510126008 CN200510126008 CN 200510126008 CN 200510126008 A CN200510126008 A CN 200510126008A CN 1975380 A CN1975380 A CN 1975380A
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- CN
- China
- Prior art keywords
- reagent
- optical detection
- acid
- sodium
- formula
- Prior art date
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- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 59
- 238000001514 detection method Methods 0.000 title claims abstract description 36
- 230000003287 optical effect Effects 0.000 title claims abstract description 27
- 108010054147 Hemoglobins Proteins 0.000 title claims abstract description 20
- 102000001554 Hemoglobins Human genes 0.000 title claims abstract description 20
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 15
- 239000003381 stabilizer Substances 0.000 claims abstract description 12
- 229910052799 carbon Inorganic materials 0.000 claims abstract description 8
- 238000006479 redox reaction Methods 0.000 claims abstract description 8
- -1 phenyl-4,5-dimethylthiazole chlorine Chemical compound 0.000 claims description 15
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 claims description 12
- NBIIXXVUZAFLBC-UHFFFAOYSA-N Phosphoric acid Chemical compound OP(O)(O)=O NBIIXXVUZAFLBC-UHFFFAOYSA-N 0.000 claims description 12
- 239000011734 sodium Substances 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 11
- MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 claims description 9
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims description 8
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 8
- 229910052708 sodium Inorganic materials 0.000 claims description 8
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- VCJMYUPGQJHHFU-UHFFFAOYSA-N iron(3+);trinitrate Chemical compound [Fe+3].[O-][N+]([O-])=O.[O-][N+]([O-])=O.[O-][N+]([O-])=O VCJMYUPGQJHHFU-UHFFFAOYSA-N 0.000 claims description 6
- 239000001632 sodium acetate Substances 0.000 claims description 6
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- BDHFUVZGWQCTTF-UHFFFAOYSA-M sulfonate Chemical compound [O-]S(=O)=O BDHFUVZGWQCTTF-UHFFFAOYSA-M 0.000 claims description 6
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- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 claims description 4
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- 235000013162 Cocos nucifera Nutrition 0.000 claims description 3
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- SNRUBQQJIBEYMU-UHFFFAOYSA-N Dodecane Natural products CCCCCCCCCCCC SNRUBQQJIBEYMU-UHFFFAOYSA-N 0.000 claims description 3
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- WDIHJSXYQDMJHN-UHFFFAOYSA-L barium chloride Chemical compound [Cl-].[Cl-].[Ba+2] WDIHJSXYQDMJHN-UHFFFAOYSA-L 0.000 claims description 3
- 229910001626 barium chloride Inorganic materials 0.000 claims description 3
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 3
- 239000004327 boric acid Substances 0.000 claims description 3
- RKBAPHPQTADBIK-UHFFFAOYSA-N cobalt;hexacyanide Chemical compound [Co].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] RKBAPHPQTADBIK-UHFFFAOYSA-N 0.000 claims description 3
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- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims description 3
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- 239000011591 potassium Substances 0.000 claims description 3
- 229910052700 potassium Inorganic materials 0.000 claims description 3
- MCJGNVYPOGVAJF-UHFFFAOYSA-N quinolin-8-ol Chemical compound C1=CN=C2C(O)=CC=CC2=C1 MCJGNVYPOGVAJF-UHFFFAOYSA-N 0.000 claims description 3
- ARIWANIATODDMH-UHFFFAOYSA-N rac-1-monolauroylglycerol Chemical compound CCCCCCCCCCCC(=O)OCC(O)CO ARIWANIATODDMH-UHFFFAOYSA-N 0.000 claims description 3
- 239000005720 sucrose Substances 0.000 claims description 3
- WCTAGTRAWPDFQO-UHFFFAOYSA-K trisodium;hydrogen carbonate;carbonate Chemical compound [Na+].[Na+].[Na+].OC([O-])=O.[O-]C([O-])=O WCTAGTRAWPDFQO-UHFFFAOYSA-K 0.000 claims description 3
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- MKHVZQXYWACUQC-UHFFFAOYSA-N bis(2-hydroxyethyl)azanium;dodecyl sulfate Chemical compound OCCNCCO.CCCCCCCCCCCCOS(O)(=O)=O MKHVZQXYWACUQC-UHFFFAOYSA-N 0.000 claims description 2
- 229910017052 cobalt Inorganic materials 0.000 claims description 2
- 239000010941 cobalt Substances 0.000 claims description 2
- GUTLYIVDDKVIGB-UHFFFAOYSA-N cobalt atom Chemical compound [Co] GUTLYIVDDKVIGB-UHFFFAOYSA-N 0.000 claims description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims 3
- 238000000354 decomposition reaction Methods 0.000 claims 3
- UZGKAASZIMOAMU-UHFFFAOYSA-N 124177-85-1 Chemical class NP(=O)=O UZGKAASZIMOAMU-UHFFFAOYSA-N 0.000 claims 1
- GLXHFRGBLGCWIK-UHFFFAOYSA-N 2-[carbamimidoyl(methyl)amino]tetradecanoic acid Chemical compound C(CCCCCCCCCCC)C(C(=O)O)N(C)C(N)=N GLXHFRGBLGCWIK-UHFFFAOYSA-N 0.000 claims 1
- QTBSBXVTEAMEQO-UHFFFAOYSA-M Acetate Chemical compound CC([O-])=O QTBSBXVTEAMEQO-UHFFFAOYSA-M 0.000 claims 1
- SYWNIVZVLMPODI-UHFFFAOYSA-N C(=CC=CC)[Fe] Chemical compound C(=CC=CC)[Fe] SYWNIVZVLMPODI-UHFFFAOYSA-N 0.000 claims 1
- OAWFRYAVNUZLQB-UHFFFAOYSA-N C(CCCCCCCCCCCCCCCCC)C1=CC=C(C)C=C1.[Na] Chemical compound C(CCCCCCCCCCCCCCCCC)C1=CC=C(C)C=C1.[Na] OAWFRYAVNUZLQB-UHFFFAOYSA-N 0.000 claims 1
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- IOVCWXUNBOPUCH-UHFFFAOYSA-N Nitrous acid Chemical compound ON=O IOVCWXUNBOPUCH-UHFFFAOYSA-N 0.000 claims 1
- OFOBLEOULBTSOW-UHFFFAOYSA-N Propanedioic acid Natural products OC(=O)CC(O)=O OFOBLEOULBTSOW-UHFFFAOYSA-N 0.000 claims 1
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- 125000001931 aliphatic group Chemical group 0.000 claims 1
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- 235000013905 glycine and its sodium salt Nutrition 0.000 claims 1
- 229910000358 iron sulfate Inorganic materials 0.000 claims 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims 1
- UETZVSHORCDDTH-UHFFFAOYSA-N iron(2+);hexacyanide Chemical compound [Fe+2].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-].N#[C-] UETZVSHORCDDTH-UHFFFAOYSA-N 0.000 claims 1
- VZCYOOQTPOCHFL-UPHRSURJSA-N maleic acid Chemical compound OC(=O)\C=C/C(O)=O VZCYOOQTPOCHFL-UPHRSURJSA-N 0.000 claims 1
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- 238000010561 standard procedure Methods 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
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Abstract
本发明涉及检测试剂，公开了一种测定人体血红蛋白的光学检测用试剂配方。该试剂配方不含剧毒药品氰化钾，包括至少一种氧化还原反应试剂和至少一种多碳裂解剂，并添加至少一种有机扩散剂和至少一种无机稳定剂。该试剂具有使用安全，测量速度快、测试结果准确、灵敏度高、检测范围宽，易于配制且成本较低等优点，很适合在医学诊断上进行血红蛋白现场快速检测。The invention relates to a detection reagent and discloses a reagent formula for optical detection for measuring human hemoglobin. The reagent formula does not contain the highly toxic drug potassium cyanide, includes at least one redox reaction reagent and at least one multi-carbon splitting agent, and adds at least one organic diffusing agent and at least one inorganic stabilizer. The reagent has the advantages of safe use, fast measurement speed, accurate test results, high sensitivity, wide detection range, easy preparation and low cost, and is very suitable for on-site rapid detection of hemoglobin in medical diagnosis.
Description
技术领域technical field
本发明涉及检测试剂，属于医学临床诊断技术领域，是一种测定人体血红蛋白的光学检测用试剂配方。The invention relates to a detection reagent, belongs to the technical field of medical clinical diagnosis, and is a reagent formula for optical detection for measuring human hemoglobin.
背景技术Background technique
血红蛋白是体内有核红细胞及网织红细胞合成的一种有色素辅基的结合蛋白质，在人体内的主要作用是参与氧气的运输。Hemoglobin is a protein with pigment prosthetic groups synthesized by nucleated red blood cells and reticulocytes in the body. Its main function in the human body is to participate in the transport of oxygen.
缺铁性贫血是常见的危害人群健康的营养问题。血红蛋白含量的测定是评价人群贫血患病率和铁营养状况的基本方法之一。其测定方法以国际血液学会标准化委员会(ICSH)推荐，经世界卫生组织(WHO)认可的氰化高铁血红蛋白(HiCN)分光光度法测定为最标准的方法。但是，由于氰化高铁血红蛋白试剂中含有剧毒药品氰化钾(KCN)，虽然该试剂中用量极少，不到人体致死量，但如果大量长期使用该试剂，加上废液处理不当，势必会造成环境污染或危害人体，需要专人保管，使用不便；氰化高铁血红蛋白试剂测定时，血红蛋白需要一定的时间转化为氰化高铁血红蛋白(约需5min后比色测定)，耗时较长，且配套仪器庞大，不易携带，成本较高，很难普及推广。Iron deficiency anemia is a common nutritional problem that endangers the health of the population. The determination of hemoglobin content is one of the basic methods to evaluate the prevalence of anemia and iron nutritional status in the population. The measurement method is recommended by the Standardization Committee of the International Society of Hematology (ICSH) and approved by the World Health Organization (WHO) Spectrophotometric measurement of methemoglobin cyanide (HiCN) is the most standard method. But, because the highly toxic drug potassium cyanide (KCN) is contained in the cyanide methemoglobin reagent, although the amount used in the reagent is extremely small, less than the lethal dose for the human body, if the reagent is used in large quantities for a long time, and the waste liquid is improperly disposed of, it is bound to It will cause environmental pollution or harm the human body, and it needs to be kept by a special person, so it is inconvenient to use; when the cyanomethemoglobin reagent is tested, it takes a certain amount of time for hemoglobin to convert into cyanomethemoglobin (it takes about 5 minutes for colorimetric measurement), which takes a long time, and The matching instrument is huge, not easy to carry, and the cost is high, so it is difficult to popularize and promote.
发明内容Contents of the invention
本发明的目的，就是针对上述测试方法的不足，向临床提供一种不含氰化钾(KCN)、价格低廉、容易保存、能快速反应、显色稳定、干扰因素小、优于氰化高铁血红蛋白(HiCN)法的且与常规方法有较高相关性的测试试剂配方，从而建立一种简便快捷的血红蛋白水平现场检测方法，可解决大量的公共卫生现场工作。The purpose of the present invention is to aim at the deficiencies of the above-mentioned test method, to provide a kind of potassium cyanide-free (KCN), low in price, easy to preserve, quick response, stable color development, small interference factors, better than ferric cyanide to the clinic. The test reagent formula of the hemoglobin (HiCN) method and has a high correlation with the conventional method, so as to establish a simple and fast on-site detection method of hemoglobin level, which can solve a large number of public health field work.
本发明的再一目的是提供一种测试试剂配方，该试剂可用于医学临床定量测定血液中的血红蛋白；可进行现场快速检测，满足医学体检的需要并能有效的消除个体间的交叉感染。Another object of the present invention is to provide a test reagent formula, which can be used for quantitative determination of hemoglobin in blood in medical clinics; it can perform on-site rapid detection, meet the needs of medical examinations, and can effectively eliminate cross-infection among individuals.
为达到以上目的，本发明的技术解决方案是提供一种测定人体血红蛋白的光学检测用试剂配方，其包括至少一种氧化还原反应试剂和至少一种多碳裂解剂，并对这两种试剂进行配比优化后，添加了至少一种有机扩散剂和至少一种无机稳定剂。In order to achieve the above object, the technical solution of the present invention is to provide a kind of optical detection reagent formula for measuring human hemoglobin, which includes at least one oxidation-reduction reaction reagent and at least one multi-carbon cracking agent, and the two reagents are carried out After the ratio is optimized, at least one organic diffusing agent and at least one inorganic stabilizer are added.
所述的光学检测用试剂配方，其所述氧化还原反应试剂，是从黄钠铁矾、三氯化铁、硫酸铁、六氰合铁酸钾、硝酸铁、六氰合钴酸钾、氯化六氨合钴、六亚硝酸合钴酸钾、双环戊二烯基铁中至少选取一种组成。In the reagent formula for optical detection, the redox reaction reagent is selected from jarosite, ferric chloride, ferric sulfate, potassium hexacyanoferrate, ferric nitrate, potassium hexacyanocobaltate, chlorine At least one composition is selected from hexaammine cobalt, potassium hexanitrite cobaltate, and biscyclopentadienyl iron.
所述的光学检测用试剂配方，其所选用的氧化还原反应试剂，其浓度在8.0～12.0g/L之间，优选为9g/L。In the reagent formula for optical detection, the selected redox reaction reagent has a concentration of 8.0-12.0 g/L, preferably 9 g/L.
所述的光学检测用试剂配方，其所述多碳裂解剂，是从十二烷基肌酸钠、聚乙二醇辛基苯基醚、冰醋酸、皂角苷、苯基-4，5-二甲基噻唑氯嗡盐、十二烷基硫酸钠聚丙烯酰胺、N-乙酰氨基葡萄塘、3-[2-(4-甲氧基-苯基)-2-氧-乙基]-4，5，6，7-四氢-苯并噻唑溴化物、3-[2-(4-硝基-苯基)-2-氧-乙基]-4，5，6，7-四氢-苯并噻唑溴化物、3-2-萘-2-基-2-氧-乙基)-4，5，6，7-四氢-苯并噻唑溴化物中至少选取一种组成。In the reagent formula for optical detection, the multi-carbon cleavage agent is obtained from sodium lauryl creatine, polyethylene glycol octylphenyl ether, glacial acetic acid, saponin, phenyl-4,5 -Dimethylthiazole chloride, sodium lauryl sulfate polyacrylamide, N-acetylaminoglucose, 3-[2-(4-methoxy-phenyl)-2-oxo-ethyl]- 4,5,6,7-tetrahydro-benzothiazole bromide, 3-[2-(4-nitro-phenyl)-2-oxo-ethyl]-4,5,6,7-tetrahydro - benzothiazole bromide, 3-2-naphthalene-2-yl-2-oxo-ethyl) -4,5,6,7-tetrahydro-benzothiazole bromide at least one composition.
所述的光学检测用试剂配方，其所选用的多碳裂解剂，其浓度在10～14g/L之间，优选为13g/L。In the reagent formula for optical detection, the selected multi-carbon splitting agent has a concentration of 10-14 g/L, preferably 13 g/L.
所述的光学检测用试剂配方，其所述有机扩散剂，是从亚甲基二萘磺酸钠、甘油-桂酸酯、聚甘油脂肪酸、聚氧化乙烯烷基酚醚、十八碳酰胺、聚氧乙烯(4)山梨醇酐单月桂酸酯、曲拉通、乙二醇一丁醚、脂肪酸聚氧乙烯醚、十二烷基叔胺盐、4-乙烯基吡啶、椰子酸二乙醇胺缩合物、顺丁烯二酸二仲辛酯磺酸钠、十二烷基硫酸二乙醇胺盐、十八烷基甲苯磺酸钠、油磺酸钠中至少选取一种组成。In the reagent formulation for optical detection, the organic diffusing agent is selected from sodium methylene dinaphthalene sulfonate, glycerin-laurate, polyglycerol fatty acid, polyoxyethylene alkylphenol ether, octadecylamide, Polyoxyethylene (4) sorbitan monolaurate, triton, ethylene glycol monobutyl ether, fatty acid polyoxyethylene ether, dodecyl tertiary amine salt, 4-vinylpyridine, coconut acid diethanolamine condensation At least one composition is selected from dioctyl maleate, sodium dioctyl sulfonate, diethanolamine lauryl sulfate, sodium octadecyl toluene sulfonate, and sodium oil sulfonate.
所述的光学检测用试剂配方，其所选用的有机扩散剂，其浓度在0.10～0.50g/L之间，优选为0.50g/L。In the optical detection reagent formula, the selected organic diffusing agent has a concentration of 0.10-0.50 g/L, preferably 0.50 g/L.
所述的光学检测用试剂配方，其所述的无机稳定剂，是从凝胶、精氨酸、草酸、8-羟基喹啉、EDTA、氨水-氯化氨、Ca(H2PO4)2-CaHPO4、硼酸、磷酸、乙酸、氢氧化钠、Na2CO3-NaHCO3、NaH2PO4-Na2HPO4、乙酸钠、脱氧胆酸钠、氯化钡、三乙醇胺、蔗糖磷酸、甘氨酸、醋酸钠中至少选取一种组成。In the reagent formula for optical detection, the inorganic stabilizer is obtained from gel, arginine, oxalic acid, 8-hydroxyquinoline, EDTA, ammonia water-ammonium chloride, Ca(H 2 PO 4 ) 2 -CaHPO 4 , boric acid, phosphoric acid, acetic acid, sodium hydroxide, Na 2 CO 3 -NaHCO 3 , NaH 2 PO 4 -Na 2 HPO 4 , sodium acetate, sodium deoxycholate, barium chloride, triethanolamine, sucrose phosphoric acid, At least one composition is selected from glycine and sodium acetate.
所述的光学检测用试剂配方，其所选用的无机稳定剂，其浓度在2.0～6.0g/L之间，优选为5.0g/L。In the reagent formulation for optical detection, the selected inorganic stabilizer has a concentration of 2.0-6.0 g/L, preferably 5.0 g/L.
所述的光学检测用试剂配方，其所选用的无机稳定剂，能调节该光学检测用试剂的pH值，其pH值在6.5～7.5之间。In said optical detection reagent formula, the selected inorganic stabilizer can adjust the pH value of the optical detection reagent, and its pH value is between 6.5 and 7.5.
附图说明Description of drawings
图1是利用本发明试剂配方检测血红蛋白与ADVIA 120生化分析仪的相关性测试示意图。Figure 1 is a schematic diagram of the correlation test between the detection of hemoglobin and the ADVIA 120 biochemical analyzer using the reagent formula of the present invention.
具体实施方式Detailed ways
本发明提出了一种测定人体血红蛋白的光学检测用试剂配方，该配方至少包括以下四种试剂：The present invention proposes a reagent formula for optical detection of human hemoglobin, which comprises at least the following four reagents:
至少一种氧化还原反应试剂，所述氧化还原反应试剂从黄钠铁矾、三氯化铁、硫酸铁、六氰合铁酸钾、硝酸铁、六氰合钴酸钾、氯化六氨合钴、六亚硝酸合钴酸钾、双环戊二烯基铁中选取，所用浓度在8.0～12.0g/L选取，优选为9g/L。At least one redox reaction reagent selected from jarosite, ferric chloride, ferric sulfate, potassium hexacyanoferrate, ferric nitrate, potassium hexacyanocobaltate, hexammine chloride Cobalt, potassium hexanitrite cobaltate, and biscyclopentadienyl iron are selected, and the concentration used is selected from 8.0 to 12.0 g/L, preferably 9 g/L.
该试剂与血红蛋白反应可使最终产物发生颜色变化，颜色深浅与血液中血红蛋白成比例，利用光的反射吸收原理即可定量检测血液中血红蛋白。The reaction of the reagent with hemoglobin can change the color of the final product, and the color depth is proportional to the hemoglobin in the blood. The hemoglobin in the blood can be quantitatively detected by using the principle of light reflection and absorption.
至少一种多碳裂解剂，所述多碳裂解剂从十二烷基肌酸钠、聚乙二醇辛基苯基醚、冰醋酸、皂角苷、苯基-4，5-二甲基噻唑氯嗡盐、十二烷基硫酸钠聚丙烯酰胺、N-乙酰氨基葡萄塘、3-[2-(4-甲氧基-苯基)-2-氧-乙基]-4，5，6，7-四氢-苯并噻唑溴化物、3-[2-(4-硝基-苯基)-2-氧-乙基]-4，5，6，7-四氢-苯并噻唑溴化物、3-2-萘-2-基-2-氧-乙基)-4，5，6，7-四氢-苯并噻唑溴化物中选取，浓度在10～14g/L之间取定，优选为13g/L。At least one multi-carbon splitting agent selected from sodium lauryl creatine, polyethylene glycol octylphenyl ether, glacial acetic acid, saponin, phenyl-4,5-dimethyl Thiazole Chloride, Sodium Lauryl Sulfate Polyacrylamide, N-Acetamido Glucose, 3-[2-(4-Methoxy-Phenyl)-2-Oxy-Ethyl]-4,5, 6,7-tetrahydro-benzothiazole bromide, 3-[2-(4-nitro-phenyl)-2-oxo-ethyl]-4,5,6,7-tetrahydro-benzothiazole Bromide, 3-2-naphthalene-2-yl-2-oxo-ethyl)-4,5,6,7-tetrahydro-benzothiazole bromide, the concentration is between 10 ~ 14g/L Determined, preferably 13g/L.
该试剂的存在可对血液中的红细胞进行裂解，从而释放出血红蛋白。The presence of this reagent lyses red blood cells in the blood, releasing hemoglobin.
至少一种有机扩散剂，所述有机扩散剂从亚甲基二萘磺酸钠、甘油-桂酸酯、聚甘油脂肪酸、聚氧化乙烯烷基酚醚、十八碳酰胺、聚氧乙烯(4)山梨醇酐单月桂酸酯、曲拉通、乙二醇一丁醚、脂肪酸聚氧乙烯醚、十二烷基叔胺盐、4-乙烯基吡啶、椰子酸二乙醇胺缩合物、顺丁烯二酸二仲辛酯磺酸钠、十二烷基硫酸二乙醇胺盐、十八烷基甲苯磺酸钠、油磺酸钠中选取，浓度在0.10～0.50g/L之间取定，优选为0.50g/L。At least one organic diffusing agent, said organic diffusing agent is selected from sodium methylene dinaphthalene sulfonate, glycerol-laurate, polyglycerol fatty acid, polyoxyethylene alkylphenol ether, octadecylamide, polyoxyethylene (4 ) sorbitan monolaurate, triton, ethylene glycol monobutyl ether, fatty acid polyoxyethylene ether, dodecyl tertiary amine salt, 4-vinylpyridine, coconut acid diethanolamine condensate, butene Di-sec-octyl sodium sulfonate, sodium dodecyl sulfate diethanolamine, sodium octadecyl toluene sulfonate, sodium oil sulfonate, the concentration is between 0.10 ~ 0.50g/L, preferably 0.50g/L.
所选用的无机扩散剂可使所滴加试样能够快速散开，与反应试剂充分接触，缩短反应时间。The selected inorganic diffusing agent can quickly disperse the dropped sample, fully contact with the reaction reagent, and shorten the reaction time.
至少一种无机稳定剂，所述无机稳定剂从凝胶、精氨酸、草酸、8-羟基喹啉、EDTA、Tris-HCL、氨水-氯化氨、Ca(H2PO4)2-CaHPO4、硼酸、磷酸、乙酸、氢氧化钠、Na2CO3-NaHCO3、NaH2PO4-Na2HPO4、乙酸钠、脱氧胆酸钠、氯化钡、三乙醇胺、蔗糖磷酸、甘氨酸、醋酸钠中选取，所用浓度在2.0～6.0g/L之间取定，优选为5.0g/L。At least one inorganic stabilizer selected from gel, arginine, oxalic acid, 8-hydroxyquinoline, EDTA, Tris-HCL, ammonia-ammonium chloride, Ca(H 2 PO 4 ) 2 -CaHPO 4. Boric acid, phosphoric acid, acetic acid, sodium hydroxide, Na 2 CO 3 -NaHCO 3 , NaH 2 PO 4 -Na 2 HPO 4 , sodium acetate, sodium deoxycholate, barium chloride, triethanolamine, sucrose phosphoric acid, glycine, Choose from sodium acetate, the concentration used is between 2.0-6.0g/L, preferably 5.0g/L.
所用无机稳定剂可增加从红细胞中释放出来的血红蛋白分子的稳定性，使显色稳定，并且此无机稳定剂还有调节试剂pH的功能，调节试剂pH值在6.5～7.5之间。The inorganic stabilizer used can increase the stability of the hemoglobin molecules released from the red blood cells to stabilize the color development, and the inorganic stabilizer also has the function of adjusting the pH of the reagent, and the pH value of the adjusted reagent is between 6.5 and 7.5.
下面结合具体实施例对本发明进行描述：The present invention is described below in conjunction with specific embodiment:
实施例1Example 1
以下列化合物所示浓度制备了一种试剂：A reagent was prepared at the concentrations indicated for the following compounds:
化合物 浓度Compound Concentration
六氰合铁酸钾 9g/LPotassium hexacyanoferrate 9g/L
皂角苷 13g/LSaponin 13g/L
NaH2PO4-Na2HPO4 5.0g/LNaH 2 PO 4 -Na 2 HPO 4 5.0g/L
曲拉通X-100 0.50g/LTriton X-100 0.50g/L
将以上试剂按所示浓度进行混合，并调节pH在6.5～7.5之间，从而制备出一种血红蛋白测试试剂。利用该试剂，通过光学检测，在北京医院临床检验检疫中心与ADVIA 120生化检测仪做对比实验，响应时间60s，对比结果如图1所示，两种试剂测试结果相关系数达到0.986，获得了较好的检测效果，可以满足现场快速检测的需要。Mix the above reagents according to the indicated concentrations, and adjust the pH between 6.5 and 7.5 to prepare a hemoglobin test reagent. Using this reagent, through optical detection, a comparative experiment was done with the ADVIA 120 biochemical detector at the Clinical Inspection and Quarantine Center of Beijing Hospital. The response time was 60s. Good detection effect can meet the needs of on-site rapid detection.
以上实施例只是为了说明本发明，而不是对本发明的限制。在上述说明的基础上，可以对本发明作许多改进和改变。在所附权利要求书的范围内，本发明可以有不同于上述的其它实现方式，选用其它试剂、改变试剂浓度等方法均在本发明专利要求范围之内。The above examples are only for illustrating the present invention, rather than limiting the present invention. Many modifications and variations of the present invention are possible on the basis of the above description. Within the scope of the appended claims, the present invention can have other implementations different from the above, and methods such as selecting other reagents and changing the concentration of reagents are all within the scope of the patent requirements of the present invention.
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| CN101828112A (en) * | 2007-10-16 | 2010-09-08 | 荣研化学株式会社 | Method for stabilizing hemoprotein and preservation solution |
| CN102300641A (en) * | 2009-01-27 | 2011-12-28 | 皇家飞利浦电子股份有限公司 | Microfluidic device for full blood count |
| CN103913454A (en) * | 2014-04-04 | 2014-07-09 | 重庆医科大学 | Preparation method of color chart for rapidly detecting content of methemoglobin |
| CN106706891A (en) * | 2017-01-03 | 2017-05-24 | 长沙中生众捷生物技术有限公司 | Hemoglobin detection reagent and hemoglobin detection paper |
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| CN101828112A (en) * | 2007-10-16 | 2010-09-08 | 荣研化学株式会社 | Method for stabilizing hemoprotein and preservation solution |
| CN101828112B (en) * | 2007-10-16 | 2013-11-13 | 荣研化学株式会社 | Method for stabilizing hemoprotein |
| CN102300641A (en) * | 2009-01-27 | 2011-12-28 | 皇家飞利浦电子股份有限公司 | Microfluidic device for full blood count |
| US10191054B2 (en) | 2009-01-27 | 2019-01-29 | Koninklijke Philips N.V. | Microfluidic device for full blood count |
| CN103913454A (en) * | 2014-04-04 | 2014-07-09 | 重庆医科大学 | Preparation method of color chart for rapidly detecting content of methemoglobin |
| CN103913454B (en) * | 2014-04-04 | 2017-01-25 | 重庆医科大学 | Preparation method of color chart for rapidly detecting content of methemoglobin |
| CN106706891A (en) * | 2017-01-03 | 2017-05-24 | 长沙中生众捷生物技术有限公司 | Hemoglobin detection reagent and hemoglobin detection paper |
| CN106706891B (en) * | 2017-01-03 | 2019-03-01 | 长沙中生众捷生物技术有限公司 | The detection reagent of hemoglobin and the Test paper of hemoglobin |
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