CN1946895A - Amylose starch products as sizing agents for textile yarns - Google Patents

Abstract

The invention relates to the use of chemically unmodified amylose-type starch products as sizing agent for sizing natural and/or synthetic yarns. The invention also relates to a process for sizing natural and/or synthetic yarns using chemically modified amylose-type starch products as sizing agent.

Description

Amylose products as sizing agents for textile yarns

field of invention

The present invention relates to the use of natural chemically unmodified amylose-type starches as sizing agents for natural and/or synthetic textile yarns and textile mixed yarns and methods for sizing textile yarns using these starch products. The present invention also relates to the use of chemically modified amylose-type starches as sizing agents for natural and/or synthetic textile yarns and yarns of textile blends and the use of chemically modified amylose-type starch products for sizing textile yarns Methods.

Background technique

Woven fabrics are two-dimensional objects composed of fibers in the form of perpendicularly intersecting threads (warp and weft) produced using the shed forming method. While each weft yarn is only briefly pulled as it is placed, the warp yarns are subjected to repeated stress each time a weft yarn is inserted and each time the shed is changed. The warp yarns are subjected to pressure in the form of yarn-to-metal wear when the reed pushes the weft, yarn-to-yarn wear during shedding changes, and stress from the cyclic tensioning process. Warp yarns are generally unable to withstand these extreme stresses and must therefore be provided with a protective coating (sizing agent) which adheres to the fibers forming an elastic film that resists abrasion. For commonly used fiber yarns, sizing agents have the effect of making the yarn resistant to the frictional processes that occur in weaving. The resulting protruding fibers adhere to the yarn body, thereby avoiding their interception or entanglement with adjacent warp yarns. The overall increase in wire tensile strength of about 20% is of little significance, while the increase in strength at the weakest point is critical.

The sizing must adhere strongly to the fibres, its film properties should be largely independent of climatic conditions, especially atmospheric humidity, and unaffected by fiber finishes and sizing additives. The elongation of the warp yarns should not be reduced by the presence of the sizing agent.

After the gray cloth is woven, the effect of the sizing agent has been completed. Since sizing agents generally have an adverse effect on subsequent finishing processes, they must be completely removed. Removal of cold water soluble sizing agents is easy, but starch products are insoluble in cold water and require prior enzymatic or oxidative breakdown prior to the desizing stage. Removal of sizing agents can create specific wastewater treatment problems for finishing plants.

A wide variety of chemicals are used as sizing agents. can be divided into two categories

1) Macromolecular natural products and their derivatives: starch and starch derivatives, carboxymethylcellulose, galactomannan and tamarind powder derivatives

2) Synthetic polymers: poly(vinyl alcohol), poly(meth)acrylates, polyester condensates and polyvinyl compounds

The increase of synthetic fibers and the development of textile technology have promoted the development of synthetic sizing agents.

The sizing agents used must meet various requirements, such as good permeability, good adhesion, good film-forming properties and the ability to form elastic sized films. Suitable sizing agents impart desirable properties to the sized yarn, such as high durability (abrasion resistance), high spinning efficiency and easy wash-out properties of the woven product.

About 70% of the sizing agents consumed worldwide are starch and starch derivatives.

Therefore, starch and its derivatives are the most important class of sizing agents in terms of overall consumption. This is due to their low price point, good sizing and worldwide availability. The raw material basis for such pastes is native starch, a polysaccharide based on α-D-glucopyranose. Starch is not a single chemical substance, but consists of two structurally distinct polymers (amylose and amylopectin). Amylose consists of chains of glucose units linked by α-1,4-glycosidic bonds, while amylopectin also contains α-1,6-glycosidic bonds that lead to polymer chain branching, see J.A. Radley: Starch and its Derivatives, Chapmann & Hall, London 1968; M.W.Rutenberg in R.L.Davidson (editor): Handbook of Water-Soluble Gums and Resins, McGraw-Hill, New York 1980, chap.22; J.BeMiller in R.L.Whistler, J.N.BeMiller (editor): Industrial Gums , Academic Press, San Diego 1993, 579 pages; G. Tegge: Strke und Strkederivate, Behr's Verlag, Hamburg 1984.

Amylopectin is the main component of starch, accounting for 73% to 86% of the total amount depending on the type of starch. The degree of polymerization of amylopectin is about 6000 to ¹⁰⁶ glucose units, and that of amylose is about 100 to 1000 glucose units. The most important sizing agents are potato, corn and tapioca starches. Wheat, rice and sago starches can also be used. The properties of these starches are determined by the amylose/amylopectin ratio, the degree of polymerization of these two components, and the size and fine structure of the starch granules. These parameters determine the behavior of swelling and dissolution as well as the properties of the film.

Native starch is insoluble in cold water due to its hydrogen bonds linking parallel polymer chains. Starch can be caused to "dissolve" by heating. Starch granules first absorb water until they swell to their maximum limit. Above a temperature unique to each type of starch and known as the gelatinization temperature, the starch granules swell and form a gel. The viscosity increases to a maximum limit and gradually decreases to a limit value as the dissolved polymer molecules disperse. Complete dissolution of individual molecules of starch granules occurs only above 100°C. The viscosity value in slurry applications is important as it has a considerable effect on liquid pick-up.

In storage and as the temperature drops, the starch paste solidifies into a slurry-like mass. This retrograde effect is caused by the extension of the molecular chains, the parallel alignment of the chains and the formation of hydrogen bonds between adjacent chains, accompanied by the loss of bound water (Tegge, 1984). This retrograde action has a detrimental effect on the sizing agent, resulting in unfavorable storage properties, crust formation, on-shaft deposit formation and reduced adhesion. Therefore, native starch is increasingly replaced by starch derivatives.

Native potato starch, eg, consists of about 80% amylopectin (I) and 20% amylose (II).

(I) Representative structure of amylopectin, including (1,6)-α-D branch point

(II) Representative structure of linear amylose

Both polymers exist as particles and are insoluble in water at room temperature. When the starch suspension is heated, the hydrogen bonds between the amylopectin and amylose chains weaken and are eventually replaced by interactions (hydrogen bonds) with water molecules. From about 61°C, the granules start to swell and water molecules penetrate the starch. Once dissolved, amylopectin is viscosity stable whereas amylose is very prone to gel formation. In this gel formation, the amylose forms a double helix which then aggregates and forms a three-dimensional network.

The use of various chemically modified starch products as sizing agents for textile yarns has been thoroughly described in K.W. Kirby; Textile Industry, in the book by O.B. Wurzburg (ed.): Modified Starches: Properties and Uses CRC Press Inc. Boca Raton , Florida, 1986, pp. 229-252. Chemically modified starches mentioned are acid-modified starches, oxidized starches, crosslinked starches, starch ethers and starch esters. Various types of starches such as corn starch, potato starch, tapioca starch and wheat starch can be used as starting materials for modification.

Starch is well known for its use in sizing cotton fibers and has a wide variety of other industrial uses. In recent years, many synthetic fibers have come into use, and it has been difficult to find relatively inexpensive sizing compositions suitable for sizing a wide variety of such fibers, including cotton and synthetic fiber blends. It is especially difficult to provide suitable inexpensive sizing compositions for blends of polyester fibers and cotton. It is conventional practice to size fibers in the form of threads or yarns prior to weaving. The sized threads or yarns are then spun into cloth, after which the size is removed by washing with detergent or enzymatic treatment. A satisfactory sizing composition is capable of providing suitable lubrication and resistance during weaving, while being easily removable after weaving.

All modified starches that have lost their original properties are called starch derivatives. These starch derivatives include low-thickness starches, dextrins, starch esters and starch ethers.

Starches of low consistency are produced by acid hydrolysis or oxidative degradation in aqueous suspension, and dextrins are usually produced by thermal depolymerization in acid. They gel at low temperatures, impart low viscosity to solutions and are soluble at high concentrations. Furthermore, the use of them facilitates the production of solutions with a predetermined viscosity, the retrograde tendency of which is largely reduced.

The main starch esters used in the size are starch phosphate (starch phosphate) and starch acetate (acetylated starch). These starch derivatives are usually not only esterified, but also depolymerized, resulting in lower solution viscosity and reduced retrograde action. In general, they give better sizing effects than low consistency starches.

The three most important types of starch ethers are hydroxyethyl, hydroxypropyl, and carboxymethyl starches, which are produced by reacting starch with ethylene oxide, propylene oxide, and chloroacetic acid or sodium chloroacetate, respectively, in the presence of caustic soda . These starch derivatives generally have a degree of substitution of about 0.1 or less. Due to its ionic character, sodium carboxymethyl starch is soluble in cold water and therefore does not require enzymatic desizing. Likewise, starch ethers have a better sizing effect than simply depolymerized starch derivatives.

The mechanical properties of films formed from starch solutions or starch derivatives depend on the degree of hydration (the degree of hydration is determined by the relative humidity of the air in the textile mill) and the ratio of amylose to amylopectin and the type of modified starch.

The main use of starch and starch derivatives is for sizing pure cotton yarns and yarns blended with other fibres. Many sizing formulations consisting exclusively or predominantly of starch or starch derivatives are used for these yarns. The adhesion of starch and its derivatives to cotton is relatively strong, see J. Trauter, M. Laupichler, Melliand Textilber. 57 (1976) 375, 443, 545, 615, 713, 797, 875, 979; 58 (1977) 23, 111. J. Trauter, H. Bauter, B. Rueβ, M. Laupichler, Textilbetrieb (Würzburg), 96 (1978) 46; J. Trauter, TPI Text. Prax. Int. 44 (1989) 1297. Cotton yarns spun on high-speed looms or yarns mixed with a high proportion of synthetic fibers must be sized with additional sizing formulations containing carboxymethylcellulose, polyvinyl alcohol or polymethacrylate to improve the sizing effect. Starch is detected by blue staining with iodine, a reaction also used for the semiquantitative determination of residual size content (P. Wurster, G. Schmidt, Melliand Textilber. 68 (1987) 581).

Contents of the invention

It is an object of the present invention to provide sizing agents based on chemically unmodified amylose-type starches which provide highly desirable sizing properties, weaving properties of sized yarns and wash-out properties of woven fabrics.

It is also an object of the present invention to provide sizing agents in which the amylose-type starch is chemically modified.

According to the invention, this object is achieved by using amylose-type starch having an amylose content of not less than 50% as a sizing agent.

Amylose potato starch with an amylose content of about 70% produced by transgenic potato plants as described in Example 1 was formulated as described in Example 2 and tested as described in Example 3 as a sizing agent. Furthermore, its desizing properties were analyzed, see Example 3.

Formulations containing amylose-type starch produced from genetically modified potato plants as described in Example 1 and having an amylose content of about 70% showed a significant increase in sizing performance compared to conventional potato starch, reflecting For higher abrasion resistance and better desizing characteristics. Chemically unmodified amylose-type starches have properties equivalent to the best commercially available reference sample, which is chemically modified and optimized.

The term "high amylose starch" refers to any starch or starch fraction containing not less than about 50% (by weight) amylose. Examples are "Nepo" amylose (the amylose portion is corn starch); "Superlose" (the amylose portion is potato starch); "Amylomaize" or "Amylon" (high amylose corn starch containing about 54% amylose); and Amylomaize VII (high amylose corn starch, containing about 73.3% amylose). Amylomaize VIII, which has an amylose content of about 85%, can also be used. The starch may be of any origin such as corn, wheat, potato, waxy corn, tapioca, sago or rice.

For sizing textile fibers, a typical sizing composition can be prepared by mixing 100 lbs of the amphoteric starch prepared according to the present invention with 100 gallons of water, preferably with the addition of 5 lbs of petroleum wax, followed by heating to gelatinization temperature. A thread or yarn to be sized, for example a thread or yarn comprising 65% polyester fibers (polyethylene terephthalate) and 35% cotton fibers, is passed through the composition to be sized. Use of the sizing composition can increase the yardage of textile material between loom stop changes.

After spinning, the size can be removed by enzymatic treatment in the usual manner or by washing with detergent-containing water.

The compositions according to the invention can also be used in other applications, such as finishing of textiles, dyeing of textiles and paper, sizing of paper, application of pigments or coatings to textiles and paper.

Amylose-type starch with an amylose content of not less than 50% can be used as a sizing agent according to the present invention to size 65% polyethylene terephthalate, 35% combed cotton yarn, rayon and other synthetic fibers Yarns or mixtures thereof, such as (but not limited to) natural fibers such as cotton, wool.

Due to its linearity, amylose is able to form elastic films with excellent sizing yarn functionality. It is important to prevent gelation in this process, as gelation will result in an insoluble film and shrinkage due to crystallization. Therefore, amylose was substituted with hydroxyethyl, hydroxypropyl or carboxymethyl groups to produce amorphous, highly soluble films or amylose was mixed with polymethylacrylate slurry to prevent the Subsequent retrograde action.

It has been found that this object is achieved according to the invention by preferably using chemically unmodified or modified amylose-type potato starches as sizing agents for natural and/or synthetic textile yarns. The following is a brief description of the characteristics of amylose potato starch.

Potato starch granules isolated from potato tubers typically contain about 20% amylose and 80% amylopectin (weight percent based on dry matter). However, in the past 10 years, potato plants have been successfully bred by genetic modification to form starch granules in potato tubers consisting of more than 50% by weight (on a dry matter basis) of amylose, preferably More than 70% amylose, more preferably more than 90% amylose by weight.

Various enzymes are catalytically active in the formation of plant starch granules. Among them, granule-bound starch synthase (GBSS) is involved in the formation of amylose. The synthesis of GBSS enzyme depends on the activity of the gene encoding GBSS enzyme. Expression of the specific genes starch branching enzyme 1 (SBE1 ) and starch branching enzyme 2 (SBE2) that abolish or inhibit amylopectin biosynthesis in, for example, potato plants results in complete loss or inhibition of amylopectin biosynthesis. Elimination of these genes is preferably achieved by genetic modification of potato plant material.

The expression of the SBE1 and SBE2 genes can also be eliminated or suppressed in potato plants, especially in their tubers, using antisense technology, see Example 1. Potatoes are described in patent applications WO 92/11375, WO 97/20040, WO 92/14827, WO 95/26407 and WO 96/34968 and in US patents US 5,856,467, US 6,169,226, US 6,469,231, US 6,215,042, US 6,570,066 and US 6,103,8 Methods of genetic modification.

It has been found that by applying genetic modifications it is possible to breed and grow potatoes whose starch granules contain little or substantially no amylopectin.

The term "amylose potato starch" is understood here to mean potato starch granules isolated from potato tubers which have an amylose content based on dry matter by weight of at least 50%. "Chemically modified amylose starch" is understood here to mean products obtained by chemical modification of amylose starch by acid modification, oxidation, esterification, etherification, graft polymerization and/or crosslinking . Before, during or after chemical modification, the amylose-type starch may also be physically modified (for example by roller drying, extrusion or moist heat treatment) or enzymatically modified. Methods for preparing various chemically modified starches are described in O.B. Wurzburg (ed.), Modified Starches: Properties and Uses; CRC Press Inc. Boca Raton, Florida, 1986. These methods can also be used to prepare chemically modified amylose-type potato starches for use as sizing agents according to the present invention.

An aqueous solution of amylose-type starch as a sizing agent, also known as a sizing solution or a sizing paste, can be prepared by conventional methods, for example using open or closed boiling equipment. The treatment of textile yarns with the aqueous solution of the chemically modified amylose-type potato starch product according to the invention can be carried out by conventional methods for sizing yarns. For example, the yarn may be passed continuously through the sizing solution or the sizing solution may be applied to the yarn by spraying or roller means. After passing through the sizing, the coating of the yarn is compacted, for example between two rollers. Next, the compacted yarn is dried in heated cylinders or hot air.

Chemically modified amylose-type starches have been found to be very suitable as sizing agents for textile yarns. The yarns sized according to the invention have good resistance to mechanical influences (high resistance to shear forces) due to the strong, elastic, smooth coating. Excellent results (high spinning efficiency) can be obtained using these yarns in textile mills.

As previously stated, the present invention relates to methods of sizing textile yarns. The term yarn therein is understood herein in the most general sense and is considered to include all cotton threads or yarns of cotton material that occur in textile mills. They may consist of continuous threads of natural cotton, or of cotton fibers and/or semi-synthetic cotton mixed with polyester, either twisted or untwisted.

The following examples will further illustrate the invention. According to these examples, some characteristic properties of the sized yarns were examined.

When sizing textile fibers, a typical sizing composition can be prepared by mixing 100 pounds of amphoteric starch prepared according to the present invention with 100 gallons of water, preferably with the addition of 5 pounds of petroleum wax, followed by heating to gelatinization temperature. The thread or yarn to be sized, for example a thread or yarn comprising 65% polyester fibers (polyethylene terephthalate) and 35% cotton fibers, is passed through the composition to be sized.

Alternative sizing agents may be prepared by adding neutralized (eg with ammonia or sodium hydroxide) poly(meth)acrylate-based emulsion polymers to starch containing sizing formulations. Preferred copolymer compositions (weight percent) of poly(meth)acrylate polymers range from:

0-10% Acrylic

                  0-20% methacrylic acid

                                                                                                     

                                                                                           

                                                                               

20-70% Methyl Acrylate

In order to obtain a good penetration of the sizing agent into the yarn, the sizing solution is preferably kept at a temperature of 30 to 90°C. The concentration of the sizing agent in the sizing solution is preferably between 2% and 20% by weight. The amount of sizing agent absorbed by the yarn (absorption; weighing) is preferably between 2% and 30% by weight of sizing agent (dry matter) based on the yarn (dry matter). In addition to the chemically modified amylose-type potato starch, the sizing solutions used may also contain conventional, minor auxiliary substances in the sizing process, such as waxes, fats, defoamers, antistatic agents and plasticizers. The sizing solution can additionally contain other sizing agents, such as polyvinyl alcohol, polymethacrylate or carboxymethylcellulose.

Use of the sizing composition can increase the yardage of textile material between loom stop changes. After spinning, the size can be removed by enzymatic treatment in the usual manner or by washing with water containing a detergent.

The compositions according to the invention can also be used in other applications, such as finishing of textiles, dyeing of textiles and paper, sizing of paper, application of pigments or coatings to textiles and paper.

Example 1

Transgenic Potato Plants - Potato (Solanum tuberosum) AM 99-2003

High amylose potato lines can be generated, for example, by using antisense, RNAi or antibody technology targeting two starch branching enzymes, starch branching enzyme 1 (SBE1 ) and starch branching enzyme 2 (SBE2).

High amylose potato line AM99-2003 was generated by inhibiting starch branching enzyme activity in the parental line Dinamo. Transformations were performed using constructs of SBE1 and SBE2 in antisense orientation driven by the gbss promoter. The nucleic acid sequence of the gbss promoter is published in EP 0 563 189.

Use Spel (blunt end) and Xbal to cut pBluescript containing the 3' end 1620bp fragment of Sbe1 between EcoRV and Spel, and connect a piece of Sbe2 3' end 1243bp Sstl (blunt end) and Xbal cut fragment. The complex of Sbe2 and Sbe1 was cut with EcoRV and Xbal and ligated into the binary vector pHo3.1 cut with Smal and Xbal. The final carrier is called pHAbe12A, see Fig. 1 and nucleotide sequence SEQ ID NO 1.pHo3.1 is based on pGPTVKan (Becker, D. et al., Plant Molecular Biology 20 (1992), 1195-1197) construction, it is in pGPTVKan The HindIII site clone added the 987bp gbss promoter and deleted the uidA gene through SmaI and Sstl.

The parental line Dinamo was transformed with construct pHAbe12A and transgenic lines were screened as described in US 6,169,226. Transgenic lines were grown and analyzed for amylose production as described by Morrison, W.R. and Laignelet, B., J. Cereal Sci. 1 (1983), 9-20. Transgenic lines are selected that produce amylose-type starch with an amylose content greater than or equal to 70%. Amylose-type starch was isolated and purified from the transgenic potato plants according to conventional methods well known in starch factories.

Example 2

preparation

Formulations containing the above-mentioned amylose-type potato starch for sizing experiments were produced according to the following recipe. formula 1 2 3 water (g) 860 860 860 PHAS 2012(g) 70 Natural Potato Starch (g) 70 Emsize E9 70 BASF Size CE 70 70 70 Total (g) 1000 1000 1000 Refractometer °Brix 6.8 8.2 7.8 Viscosity (viscosity cup, secondary) 33 25 13

"PHAS 2012" is a genetically modified amylose-type potato starch with a solid content of 83.5% (moisture content 16.5%); amylose content of 70%, according to eg Morrison, W.R. and Laignelet, B., J. Measured by the method described in Sci.1 (1983), 9-20. "Native potato starch" is ordinary potato starch (Emsland Störke GmbH, Germany) without any (chemical or thermal) modification, comprising about 20% amylose and 80% amylopectin with a solids content of 84.7% (moisture content 15.3%);

"BASF Size CE" (BASF Aktiengesellschaft, Germany) is a polyacrylate based sizing agent with a solids content of 25%;

"Emsize E9" is a sizing agent based on chemically modified potato starch (propoxylated, 0.2-0.3 degrees/per repeating unit). The solids content is about 85% (moisture content 15%).

The amounts of the individual components in the formulation can vary accordingly as follows:

Depending on textile performance needs, the formulation of the above size liquid can be varied mainly from 100% starch (required for low performance) to 100% polymethacrylate (required for high performance). Typically polymethacrylate based sizes are added to the starch size in a ratio between 10 (starch): 1 (polymethacrylate) and 1 :1 as a compromise between cost and performance.

Example 3

test

In comparative tests, the above-mentioned amylose-type starch (Recipe 1: PHAS 2012) was compared to native potato starch (Recipe 2) and the best commercially available starch sizing product (Recipe 3: Emsize E9) (based on chemically modified starch (propoxylation, 0.2-0.3 degrees/per repeat unit)) for testing. All starch components are formulated in "BASF size CE". The abrasion resistance and desizing properties were determined.

Test according to the following methods:

wear test

The sizing effect is of decisive importance for the textile properties of warp threads. This effect is closely linked to the abrasion resistance of the sized yarn. We determined the abrasion resistance of the yarns with a Zweigle abrasion tester (G552 abrasion tester, Zweigle Textilprüfmaschinen GmbH & Co. KG, Germany).

A standard easy-change sandpaper is tensioned on a shaft that moves linearly in the direction of the line. Twenty stressed threads (cotton component yarn, UK count Ne 12) are overlaid on the shaft and frayed at the same speed and under the same pressure until they break. To ensure that fiber deposits in the wear do not affect the wear behavior, the shaft is advanced after each stroke. Read the number of wear strokes before breaking from the counter and calculate its average value. The higher the abrasion number obtained, the higher the abrasion resistance of the yarn.

The values obtained in the abrasion test are relative only and must be considered in comparison to unsized warp yarn from the same lot or to warp yarn from the same lot sized with another formulation.

The following results are obtained:

Wear Count (Cotton Yarn, Ne 12) formula unsized 1 2 3 Filled slurry (solids - solids %) 7.7 7.6 7.5 Wear Count (20) 263 1752 1416 1728 Wear Count (5) 185 1001 868 998

Desizing test

Starch detection using the TEGEWA method

One of the most common tests used to determine the effectiveness of pretreatment is to detect the presence of starch size by swabbing the fabric with an iodine/potassium iodide solution. Blue staining indicates that starch size is still present in the fabric.

When applying this test, be aware that even if only 1% of the original starch size is still present, ie even if 99% of the original starch size has been removed, the blue stain will still be visible. However, such traces of residual size no longer have any influence on the behavior of the pretreated goods in dyeing and printing.

The TEGEWA purple scale with shades representing 9 grades can provide a remedy for this. A grade of 1 represents the worst desizing, while a grade of 9 represents nearly complete desizing.

method

The fabric samples were soaked in 0.005 mol/l iodine solution for 1 minute. This was followed by a brief water wash, dabbing with filter paper and immediate comparison to the purple score.

Preparation of iodine solution

1. Dissolve 10g potassium iodide in 100ml distilled water

2. Add and dissolve 0.635g of iodine in this solution

3. Make up to 800ml with water

4. Make up to 1000ml with ethanol

Desizing properties formula 1 2 3 Slurry washed off within 5 minutes (%) 64.7 67.5 66.7 Slurry washed off within 10 minutes (%) 76.3 70.3 77.3 Slurry washed off within 30 minutes (%) 96.5 76.8 95.5 TEGEWA 5 minutes 1 1 1 TEGEWA 10 minutes 2 1 2 TEGEWA 30 minutes 3 2 3

A formulation containing amylose-type starch produced from a genetically modified potato plant as described in Example 1 and having an amylose content of about 70% exhibited a significantly increased sizing performance as indicated by the achievement of more High abrasion resistance and better desizing characteristics. The chemically unmodified amylose-type starch has properties equivalent to the best commercially available reference sample, which is chemically modified and optimized.

sequence listing

Sequence Listing

<110> Basford Plant Science Co., Ltd.

<120> Amylose products as sizing agents for textile yarns

<130> Amylose as a sizing agent

<140>AE20040190

<141>2004-03-30

<160>1

<170> PatentIn version 2.1

<210>1

<211>15294

<212>DNA

<213> Artificial sequence

<220>

<223> Manual Sequence Description: Vectors

pHAbe12A

<400>1

ggccgggagg gttcgagaag ggggggcacc ccccttcggc gtgcgcggtc acgcgcacag 60

ggcgcagccc tggttaaaaa caaggtttat aaatattggt ttaaaagcag gttaaaagac 120

aggttagcgg tggccgaaaa acgggcggaa acccttgcaa atgctggatt ttctgcctgt 180

ggacagcccc tcaaatgtca ataggtgcgc ccctcatctg tcagcactct gcccctcaag 240

tgtcaaggat cgcgcccctc atctgtcagt agtcgcgccc ctcaagtgtc aataccgcag 300

ggcacttatc cccaggcttg tccacatcat ctgtgggaaa ctcgcgtaaa atcaggcgtt 360

ttcgccgatt tgcgaggctg gccagctcca cgtcgccggc cgaaatcgag cctgcccctc 420

atctgtcaac gccgcgccgg gtgagtcggc ccctcaagtg tcaacgtccg cccctcatct 480

gtcagtgagg gccaagtttt ccgcgaggta tccacaacgc cggcggccgc ggtgtctcgc 540

acacggcttc gacggcgttt ctggcgcgtt tgcagggcca tagacggccg ccagcccagc 600

ggcgagggca accagcccgg tgagcgtcgc aaaggcgctc ggtcttgcct tgctcgtcgg 660

tgatgtactt caccagctcc gcgaagtcgc tcttcttgat ggagcgcatg gggacgtgct 720

tggcaatcac gcgcaccccc cggccgtttt agcggctaaa aaagtcatgg ctctgccctc 780

gggcggacca cgcccatcat gaccttgcca agctcgtcct gcttctcttc gatcttcgcc 840

agcagggcga ggatcgtggc atcaccgaac cgcgccgtgc gcgggtcgtc ggtgagccag 900

agtttcagca ggccgcccag gcggcccagg tcgccattga tgcgggccag ctcgcggacg 960

tgctcatagt ccacgacgcc cgtgattttg tagccctggc cgacggccag caggtaggcc 1020

gacaggctca tgccggccgc cgccgccttt tcctcaatcg ctcttcgttc gtctggaagg 1080

cagtacacct tgataggtgg gctgcccttc ctggttggct tggtttcatc agccatccgc 1140

ttgccctcat ctgttacgcc ggcggtagcc ggccagcctc gcagagcagg attcccgttg 1200

agcaccgcca ggtgcgaata agggacagtg aagaaggaac acccgctcgc gggtggggcct 1260

acttcaccta tcctgcccgg ctgacgccgt tggatacacc aaggaaagtc tacacgaacc 1320

ctttggcaaa atcctgtata tcgtgcgaaa aaggatggat ataccgaaaa aatcgctata 1380

atgaccccga agcagggtta tgcagcggaa aagcgccacg cttcccgaag ggagaaaggc 1440

ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg 1500

gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg 1560

atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt 1620

tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc 1680

tgattctgtg gataaccgta ttaccgcctt tgagtgagct gataccgctc gccgcagccg 1740

aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa gagcgccaga aggccgccag 1800

agaggccgag cgcggccgtg aggcttggac gctagggcag ggcatgaaaa agcccgtagc 1860

gggctgctac gggcgtctga cgcggtggaa aggggggaggg gatgttgtct acatggctct 1920

gctgtagtga gtgggttgcg ctccggcagc ggtcctgatc aatcgtcacc ctttctcggt 1980

ccttcaacgt tcctgacaac gagcctcctt ttcgccaatc catcgacaat caccgcgagt 2040

ccctgctcga acgctgcgtc cggaccggct tcgtcgaagg cgtctatcgc ggcccgcaac 2100

agcggcgaga gcggagcctg ttcaacggtg ccgccgcgct cgccggcatc gctgtcgccg 2160

gcctgctcct caagcacggc cccaacagtg aagtagctga ttgtcatcag cgcattgacg 2220

gcgtccccgg ccgaaaaacc cgcctcgcag aggaagcgaa gctgcgcgtc ggccgtttcc 2280

atctgcggtg cgcccggtcg cgtgccggca tggatgcgcg cgccatcgcg gtaggcgagc 2340

agcgcctgcc tgaagctgcg ggcattcccg atcagaaatg agcgccagtc gtcgtcggct 2400

ctcggcaccg aatgcgtatg attctccgcc agcatggctt cggccagtgc gtcgagcagc 2460

gcccgcttgt tcctgaagtg ccagtaaagc gccggctgct gaacccccaa ccgttccgcc 2520

agtttgcgtg tcgtcagacc gtctacgccg acctcgttca acaggtccag ggcggcacgg 2580

atcactgtat tcggctgcaa ctttgtcatg cttgacactt tatcactgat aaacatata 2640

tgtccaccaa cttatcagtg ataaagaatc cgcgcgttca atcggaccag cggaggctgg 2700

tccggaggcc agacgtgaaa cccaacatac ccctgatcgt aattctgagc actgtcgcgc 2760

tcgacgctgt cggcatcggc ctgattatgc cggtgctgcc gggcctcctg cgcgatctgg 2820

ttcactcgaa cgacgtcacc gcccactatg gcattctgct ggcgctgtat gcgttggtgc 2880

aatttgcctg cgcacctgtg ctgggcgcgc tgtcggatcg tttcgggcgg cggccaatct 2940

tgctcgtctc gctggccggc gccaagatct ggggaaccct gtggttggca tgcacataca 3000

aatggacgaa cggataaacc ttttcacgcc cttttaaata tccgattatt ctaataaacg 3060

ctcttttctc ttaggtttac ccgccaatat atcctgtcaa acactgatag tttaaactga 3120

aggcgggaaa cgacaatctg atcatgagcg gagaattaag ggagtcacgt tatgaccccc 3180

gccgatgacg cgggacaagc cgttttacgt ttggaactga cagaaccgca acgttgaagg 3240

agccactcag ccgatctgaa ttcccgatct agtaacatag atgacaccgc gcgcgataat 3300

ttatcctagt ttgcgcgcta tattttgttt tctatcgcgt attaaatgta taattgcggg 3360

actctaatca taaaaaccca tctcataaat aacgtcatgc attacatgtt aattattaca 3420

tgcttaacgt aattcaacag aaattatatg ataatcatcg caagaccggc aacaggattc 3480

aatcttaaga aactttatg ccaaatgttt gaacgatcgg ggaaattcga gctcggtacc 3540

atcatgttac aaactttttt gctgtgagca gtagatatgg aaacccggag gacctaaagt 3600

atctgataga taaagcacat agcttgggtt tacaggttct ggtggatgta gttcacagtc 3660

atgcaagcaa taatgccact gatggcctca atggctttga tattggccaa ggttctcaag 3720

aatcctactt tcatgctgga gagcaagggt accataagtt gtgggatagc aggctgttca 3780

actatgccaa ttgggaggtt cttcgtttcc ttctttccaa cttgaggtgg tggctagaag 3840

agtataactt tgacggattt cgatttgatg gaataacttc tatgctgtat gttcatcatg 3900

gaatcaatat gggatttaca ggaaactata atgagtattt cagcgaggct acagatgttg 3960

atgctgtggt ctatttaatg ttggccaata atctgattca caagattttc ccagacgcaa 4020

ctgttattgc cgaagatgtt tctggtatgc cgggccttgg ccggcctgtt tctgagggag 4080

gaattggttt tgattaccgc ctggcaatgg caatcccaga taagtggata gattatttaa 4140

agaataaaaa tgatgaagat tggtccatga aggaagtaac atcgagtttg acaaatagga 4200

gatatacaga gaagtgtata gcatatgcgg agagccatga tcagtctatt gtcggtgaca 4260

agaccattgc atttctccta atggacaaag agatgtattc tggcatgtct tgcttgacag 4320

atgcttctcc tgttattgat cgaggaattg cgcttcacaa gatgatccat tttttcacaa 4380

tggccttggg aggagagggg tacctcaatt tcatgggtaa cgagtttggc catcctgagt 4440

ggattgactt ccctagagag ggcaataatt ggtgttatga caaatgtaga cgccagtgga 4500

accttgcgga tagcgaacac ttgagataca agtttatgaa tgcatttgat agagctatga 4560

attcgctcga tgaaaagttc tcattcctcg catcaggaaa acagatagta agcagcatgg 4620

atgatgagaa gaaggttgtt gtgtttgaac gtggtgacct ggtatttgta ttcaacttcc 4680

acccaaataa cacatacgaa gggtataaag ttggatgtga cttgccaggg aagtacagag 4740

ttgcactgga cagtgatgct tgggaatttg gtggccatgg aagagctggt catgatgttg 4800

accatttcac atcaccagaa ggaatacctg gagttccaga aacaaatttc aatggtcgtc 4860

caaattcctt caaagtgctg tctcctgcgc gaacatgtgt ggcttattac agagttgacg 4920

aacgcatgtc agaaactgaa gtttaccaga cagacatttc tagtgagcta ctaccaacag 4980

ccaatatcga ggagagtgac gagaaactta aagattcgtt atctacaaat atcagtaacg 5040

ttgacgaact catgtcagaa actgaagttt accagacaga catttctagt gagctactac 5100

caacagccag tatcgaggag agtgacgaga aacttaaaga ttcattatct acaaatatca 5160

gtacgtggtt atcattggat gtgggatcc cgcctcttta attatggaaa ctgggaggta 5220

cttaggtatc ttctctcaaa tgcgagatgg tggttggatg agttcaaatt tgatggattt 5280

agattcgatg gtgtgacatc aataatgtat actcaccacg gattatcggt gggattcact 5340

gggaactaca aggaatactt tggactcgca actgatgtgg atgctgttgt gtatctgatg 5400

ctggtcaacg atcttattca tgggcttttc cagatgcaat taccattggt gaagatgtta 5460

gcggaatgcc gacattttgt attcccgttc aagatggggg tgttggcttt gactatcggc 5520

tgcatatggc aattgctgat aaatggattg agttgctcaa gaaacgggat gaggattgga 5580

gagtgggtga tattgttcat acactgacaa atagaagatg gtcggaaaag tgtgtttcat 5640

acgctgaaag tcatgatcaa gctctagtcg gtgataaaac tatagcattc tggctgatgg 5700

acaaggatat gtatgatttt atggctctgg atagaccatc aacatcatta atagatcgtg 5760

ggatagcatt gcacaagatg attaggcttg taactatggg attaggagga gaagggtacc 5820

taaatttcat gggaaatgaa ttcggccacc ctgagtggat tgatttccct agggctgaac 5880

aacacctctc tgatggctca gtaattcccg gaaaccaatt cagttatgat aaatgcagac 5940

ggagatttga cctgggagat gcagaatatt taagataccg tgggttgcaa gaatttgacc 6000

gggctatgca gtatcttgaa gataaatatg agtttatgac ttcagaacac cagttcatat 6060

cacgaaagga tgaaggagat aggatgattg tatttgaaaa aggaaaccta gtttttgtct 6120

ttaattttca ctggacaaaa agctattcag actatcgcat aggctgcctg aagcctggaa 6180

aatacaaggt tgccttggac tcagatgatc cactttttgg tggcttcggg agaattgatc 6240

ataatgccga atatttcacc tttgaaggat ggtatgatga tcgtcctcgt tcaattatgg 6300

tgtatgcacc tagtagaaca gcagtggtct atgcactagt agacaaagaa gaagaagaag 6360

aagaagaagt agcagtagta gaagaagtag tagtagaaga agaatgaacg aacttgtgat 6420

cgcgttgaaa gatttgaagg ctacatagct ctagagtcga cctgcatgaa atcagaaata 6480

attggaggag atgagtaaaa gttaccactt gttgagctgt gtgagtgagt gagtgagaat 6540

gaggaggtgc ctgccttatttgtagcaggt ttcagtgaca cgtgtcaaga gaatagcggg 6600

tggctatccc ttagcagaag gcaactgtgg acactgtatt atagggaaat gctcatcgac 6660

agtattatgg gccctctctt tgttgattca cggctggact tcaacttggg ccttgcaatg 6720

ggcccgtccg gttctgtctc ctagtatcta aaaaactaaa ccaactccct cctaccgcta 6780

ccacttgaca ttcctatgtc tcgtgttaat taaattatta ttatagtaat taaaaataat 6840

atctaggtac tggtactggt ccctccctcc actagaatat tagttacttc ccccttagct 6900

ttgtattcca aattactgta aatatatttt ctaatttttt acgacaaaca agatctaatt 6960

atgaatgcac aattctaaag gttgaataca ttactttact tggtttagcc tatattaagt 7020

tgcattttag tattaagatt gagatgcatg gttctattac aaaattgata cactgctaaa 7080

ggaaggatgg ttaaaaacaa cattcaatgt ttgttacatt tcttcctatt gtattttttt 7140

tttaacgagc ttcccgtata catcataaca tgtctccgtt ccacttggca ggaaaaaaaa 7200

atacccaaac aggaagatac tgtcaagtat atccatagat gaggacttaa tggataggct 7260

tttcgaggat tcataaatca taatatctgg cggaggagtc aattaaatac ttgtggtttg 7320

tatcctgatt actccgtcaa cagccaaata gaaaagtttg aaaagagaga aaggatttgg 7380

tacaagatac tgttgcattt gttaagtaat gaacaaaacg gagtaacata attttctatc 7440

tcgttaaagc ttcacgctgc cgcaagcact cagggcgcaa gggctgctaa ggaagcggaa 7500

cacgtagaaa gccagtccgc agaaacggtg ctgaccccgg atgaatgtca gctactgggc 7560

tatctggaca agggaaaacg caagcgcaaa gagaaagcag gtagcttgca gtgggcttac 7620

atggcgatag ctagactggg cggttttatg gacagcaagc gaaccggaat tgccagctgg 7680

ggcgccctct ggtaaggttg ggaagccctg caaagtaaac tggatggctt tcttgccgcc 7740

aaggatctga tggcgcaggg gatcaagatc atgagcggag aattaaggga gtcacgttat 7800

gacccccgcc gatgacgcgg gacaagccgt tttacgtttg gaactgacag aaccgcaacg 7860

ttgaaggagc cactcagccg cgggtttctg gagtttaatg agctaagcac atacgtcaga 7920

aaccattatt gcgcgttcaa aagtcgccta aggtcactat cagctagcaa atatttcttg 7980

tcaaaaatgc tccactgacg ttccataaat tcccctcggt atccaattag agtctcatat 8040

tcactctcaa tccagatctc gactctagtc gagggcccat gggagcttgg attgaacaag 8100

atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg 8160

cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc 8220

cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag 8280

cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca 8340

ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat 8400

ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata 8460

cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac 8520

gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc 8580

tcgcgccagc cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg 8640

tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg 8700

gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta 8760

cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg 8820

gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct 8880

gagcgggacc caagctagct tcgacggatc ccccgatgag ctaagctagc tatatcatca 8940

atttatgtat tacacataat atcgcactca gtctttcatc tacggcaatg taccagctga 9000

tataatcagt tattgaaata tttctgaatt taaacttgca tcaataaatt tatgtttttg 9060

cttggactat aatacctgac ttgttatttt atcaataaat atttaaacta tatttctttc 9120

aagatgggaa ttaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc 9180

gttacccaac ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa 9240

gaggcccgca ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcgc ccgctccttt 9300

cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg 9360

ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga 9420

tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac 9480

gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc 9540

tatctcgggc tattcttttg atttataagg gattttgccg atttcggaac caccatcaaa 9600

caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact ctctcagggc 9660

caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa aaccacccca 9720

gtacattaaa aacgtccgca atgtgttatt aagttgtcta agcgtcaatt tgtttacacc 9780

acaatatatc ctgccaccag ccagccaaca gctccccgac cggcagctcg gcacaaaatc 9840

accactcgat acaggcagcc catcagtccg ggacggcgtc agcgggagag ccgttgtaag 9900

gcggcagact ttgctcatgt taccgatgct attcggaaga acggcaacta agctgccggg 9960

tttgaaacac ggatgatctc gcggagggta gcatgttgat tgtaacgatg acagagcgtt 10020

gctgcctgtg atcaaatatc atctccctcg cagagatccg aattatcagc cttcttattc 10080

atttctcgct taaccgtgac aggctgtcga tcttgagaac tatgccgaca taataggaaa 10140

tcgctggata aagccgctga ggaagctgag tggcgctatt tctttagaag tgaacgttga 10200

cgatatcaac tcccctatcc attgctcacc gaatggtaca ggtcggggac ccgaagttcc 10260

gactgtcggc ctgatgcatc cccggctgat cgaccccaga tctagatctg gggctgagaa 10320

agcccagtaa ggaaacaact gtaggttcga gtcgcgagat cccccggaac caaaggaagt 10380

aggttaaacc cgctccgatc aggccgagcc acgccaggcc gagaacattg gttcctgtag 10440

gcatcgggat tggcggatca aacactaaag ctactggaac gagcagaagt cctccggccg 10500

ccagttgcca ggcggtaaag gtgagcagag gcacgggagg ttgccacttg cgggtcagca 10560

cggttccgaa cgccatggaa accgcccccg ccaggcccgc tgcgacgccg acaggatcta 10620

gcgctgcgtt tggtgtcaac accaacagcg ccacgcccgc agttccgcaa atagccccca 10680

ggaccgccat caatcgtatc gggctaccta gcagagcggc agagatgaac acgaccatca 10740

gcggctgcac agcgcctacc gtcgccgcga ccccgcccgg caggcggtag accgaaataa 10800

acaacaagct ccagaatagc gaaatattaa gtgcgccgag gatgaagatg cgcatccacc 10860

agattcccgt tggaatctgt cggacgatca tcacgagcaa taaacccgcc ggcaacgccc 10920

gcagcagcat accggcgacc cctcggcctc gctgttcggg ctccacgaaa acgccggaca 10980

gatgcgcctt gtgagcgtcc ttggggccgt cctcctgttt gaagaccgac agcccaatga 11040

tctcgccgtc gatgtaggcg ccgaatgcca cggcatctcg caaccgttca gcgaacgcct 11100

ccatgggctt tttctcctcg tgctcgtaaa cggacccgaa catctctgga gctttcttca 11160

gggccgacaa tcggatctcg cggaaatcct gcacgtcggc cgctccaagc cgtcgaatct 11220

gagccttaat cacaattgtc aattttaatc ctctgtttat cggcagttcg tagagcgcgc 11280

cgtgcgtccc gagcgatact gagcgaagca agtgcgtcga gcagtgcccg cttgttcctg 11340

aaatgccagt aaagcgctgg ctgctgaacc cccagccgga actgacccca caaggcccta 11400

gcgtttgcaa tgcaccaggt catcattgac ccaggcgtgt tccaccaggc cgctgcctcg 11460

caactcttcg caggcttcgc cgacctgctc gcgccacttc ttcacgcggg tggaatccga 11520

tccgcacatg aggcggaagg tttccagctt gagcgggtac ggctcccggt gcgagctgaa 11580

atagtcgaac atccgtcggg ccgtcggcga cagcttgcgg tacttctccc atatgaattt 11640

cgtgtagtgg tcgccagcaa acagcacgac gatttcctcg tcgatcagga cctggcaacg 11700

ggacgttttc ttgccacggt ccaggacgcg gaagcggtgc agcagcgaca ccgattccag 11760

gtgcccaacg cggtcggacg tgaagcccat cgccgtcgcc tgtaggcgcg acaggcattc 11820

ctcggccttc gtgtaatacc ggccattgat cgaccagccc aggtcctggc aaagctcgta 11880

gaacgtgaag gtgatcggct cgccgatagg ggtgcgcttc gcgtactcca acacctgctg 11940

ccacaccagt tcgtcatcgt cggcccgcag ctcgacgccg gtgtaggtga tcttcacgtc 12000

cttgttgacg tggaaaatga ccttgttttg cagcgcctcg cgcgggattt tcttgttgcg 12060

cgtggtgaac agggcagagc gggccgtgtc gtttggcatc gctcgcatcg tgtccggcca 12120

cggcgcaata tcgaacaagg aaagctgcat ttccttgatc tgctgcttcg tgtgtttcag 12180

caacgcggcc tgcttggcct cgctgacctg ttttgccagg tcctcgccgg cggtttttcg 12240

cttcttggtc gtcatagttc ctcgcgtgtc gatggtcatc gacttcgcca aacctgccgc 12300

ctcctgttcg agacgacgcg aacgctccac ggcggccgat ggcgcgggca gggcagggggg 12360

agccagttgc acgctgtcgc gctcgatctt ggccgtagct tgctggacca tcgagccgac 12420

ggactggaag gtttcgcggg gcgcacgcat gacggtgcgg cttgcgatgg tttcggcatc 12480

ctcggcggaa aaccccgcgt cgatcagttc ttgcctgtat gccttccggt caaacgtccg 12540

attcattcac cctccttgcg ggattgcccc gactcacgcc ggggcaatgt gcccttattc 12600

ctgatttgac ccgcctggtg ccttggtgtc cagataatcc accttatcgg caatgaagtc 12660

ggtcccgtag accgtctggc cgtccttctc gtacttggta ttccgaatct tgccctgcac 12720

gaataccagc gaccccttgc ccaaatactt gccgtgggcc tcggcctgag agccaaaaca 12780

cttgatgcgg aagaagtcgg tgcgctcctg cttgtcgccg gcatcgttgc gccacatcta 12840

ggtactaaaa caattcatcc agtaaaatat aatattttat tttctcccaa tcaggcttga 12900

tccccagtaa gtcaaaaaat agctcgacat actgttcttc cccgatatcc tccctgatcg 12960

accggacgca gaaggcaatg tcataccact tgtccgccct gccgcttctc ccaagatcaa 13020

taaagccact tactttgcca tctttcacaa agatgttgct gtctcccagg tcgccgtggg 13080

aaaagacaag ttcctcttcg ggcttttccg tctttaaaaa atcatacagc tcgcgcggat 13140

ctttaaatgg agtgtcttct tcccagtttt cgcaatccac atcggccaga tcgttattca 13200

gtaagtaatc caattcggct aagcggctgt ctaagctatt cgtataggga caatccgata 13260

tgtcgatgga gtgaaagagc ctgatgcact ccgcatacag ctcgataatc ttttcagggc 13320

tttgttcatc ttcatactct tccgagcaaa ggacgccatc ggcctcactc atgagcagat 13380

tgctccagcc atcatgccgt tcaaagtgca ggacctttgg aacaggcagc tttccttcca 13440

gccatagcat catgtccttt tcccgttcca catcataggt ggtcccttta taccggctgt 13500

ccgtcatttt taaatatagg ttttcatttt ctcccaccag cttatatacc ttagcaggag 13560

acattccttc cgtatctttt acgcagcggt atttttcgat cagttttttc aattccggtg 13620

atattctcat tttagccatt tattatttcc ttcctctttt ctacagtatt taaagatacc 13680

ccaagaagct aattataaca agacgaactc caattcactg ttccttgcat tctaaaacct 13740

taaataccag aaaacagctt tttcaaagtt gttttcaaag ttggcgtata acatagtatc 13800

gacggagccg attttgaaac cacaattatg ggtgatgctg ccaacttact gatttagtgt 13860

atgatggtgt ttttgaggtg ctccagtggc ttctgtgtct atcagctgtc cctcctgttc 13920

agctactgac ggggtggtgc gtaacggcaa aagcaccgcc ggacatcagc gctatctctg 13980

ctctcactgc cgtaaaacat ggcaactgca gttcacttac accgcttctc aacccggtac 14040

gcaccagaaa atcattgata tggccatgaa tggcgttgga tgccgggcaa cagcccgcat 14100

tatgggcgtt ggcctcaaca cgattttacg tcacttaaaa aactcaggcc gcagtcggta 14160

acctcgcgca tacagccggg cagtgacgtc atcgtctgcg cggaaatgga cgaacagtgg 14220

ggctatgtcg gggctaaatc gcgccagcgc tggctgtttt acgcgtatga cagtctccgg 14280

aagacggttg ttgcgcacgt attcggtgaa cgcactatgg cgacgctggg gcgtcttatg 14340

agcctgctgt caccctttga cgtggtgata tggatgacgg atggctggcc gctgtatgaa 14400

tcccgcctga agggaaagct gcacgtaatc agcaagcgat atacgcagcg aattgagcgg 14460

cataacctga atctgaggca gcacctggca cggctgggac ggaagtcgct gtcgttctca 14520

aaatcggtgg agctgcatga caaagtcatc gggcattatc tgaacataaa acactatcaa 14580

taagttggag tcattaccca attatgatag aatttacaag ctataaggtt attgtcctgg 14640

gtttcaagca ttagtccatg caagttttta tgctttgccc attctataga tatattgata 14700

agcgcgctgc ctatgccttg ccccctgaaa tccttacata cggcgatatc ttctatataa 14760

aagatatatt atcttatcag tattgtcaat atattcaagg caatctgcct cctcatcctc 14820

ttcatcctct tcgtcttggt agctttttaa atatggcgct tcatagagta attctgtaaa 14880

ggtccaattc tcgttttcat acctcggtat aatcttacct atcacctcaa atggttcgct 14940

gggtttatcg cacccccgaa cacgagcacg gcacccgcga ccactatgcc aagaatgccc 15000

aaggtaaaaa ttgccggccc cgccatgaag tccgtgaatg ccccgacggc cgaagtgaag 15060

ggcaggccgc cacccaggcc gccgccctca ctgcccggca cctggtcgct gaatgtcgat 15120

gccagcacct gcggcacgtc aatgcttccg ggcgtcgcgc tcgggctgat cgcccatccc 15180

gttactgccc cgatcccggc aatggcaagg actgccagcg ctgccatttt tggggtgagg 15240

ccgttcgcgg ccgaggggcg cagcccctgg ggggatggga ggcccgcgtt agcg 15294

Claims (8)

1. A method of using amylose-type starch as a sizing agent for natural cotton yarn. 2. The method of using amylose-type starch as a sizing agent according to claim 1, characterized in that the amylose-type starch has an amylose content of not less than 50%. 3. The method of using amylose-type starch as a sizing agent according to claim 2, characterized in that the amylose-type starch is produced by transgenic potato plants. 4. according to claim 1,2 or 3 described methods using amylose type starch as sizing agent, it is characterized in that amylose type starch is used in combination with other sizing agents, for example with polyvinyl alcohol, carboxymethyl Use in combination with cellulose-based or polymethacrylate. 5. Process for sizing natural cotton yarns or mixtures of cotton and polyester yarns, characterized in that chemically unmodified amylose-type starches are used as sizing agents. 6. Process for sizing natural cotton yarns or mixtures of cotton and polyester yarns, characterized in that chemically modified amylose-type starches are used as sizing agents. 7. Process for sizing natural cotton yarns or mixtures of cotton and polyester yarns according to claim 5 or 6, characterized in that the amylose-type starch is produced by transgenic potato plants. 8. Process for sizing natural cotton yarns or cotton and polyester yarn mixtures according to claim 7, characterized in that the amylose-type starch has an amylose content of not less than 50%.