CN1704119A - Oil emulsion inactivated vaccine for parvoviral diseases of pigs - Google Patents
Oil emulsion inactivated vaccine for parvoviral diseases of pigs Download PDFInfo
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- CN1704119A CN1704119A CN 200410024754 CN200410024754A CN1704119A CN 1704119 A CN1704119 A CN 1704119A CN 200410024754 CN200410024754 CN 200410024754 CN 200410024754 A CN200410024754 A CN 200410024754A CN 1704119 A CN1704119 A CN 1704119A
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- Prior art keywords
- vaccine
- oil
- emulsion
- parvoviral
- pigs
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- 239000000839 emulsion Substances 0.000 title claims abstract description 20
- 229940031551 inactivated vaccine Drugs 0.000 title abstract description 6
- 241000282887 Suidae Species 0.000 title abstract description 3
- 201000010099 disease Diseases 0.000 title abstract 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title abstract 2
- 239000002671 adjuvant Substances 0.000 claims abstract description 21
- 241000702619 Porcine parvovirus Species 0.000 claims abstract description 12
- CEGOLXSVJUTHNZ-UHFFFAOYSA-K aluminium tristearate Chemical compound [Al+3].CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O.CCCCCCCCCCCCCCCCCC([O-])=O CEGOLXSVJUTHNZ-UHFFFAOYSA-K 0.000 claims abstract description 10
- 229960005486 vaccine Drugs 0.000 claims description 44
- 230000001804 emulsifying effect Effects 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 10
- 239000007788 liquid Substances 0.000 claims description 7
- 230000009849 deactivation Effects 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 3
- JNYAEWCLZODPBN-JGWLITMVSA-N (2r,3r,4s)-2-[(1r)-1,2-dihydroxyethyl]oxolane-3,4-diol Chemical compound OC[C@@H](O)[C@H]1OC[C@H](O)[C@H]1O JNYAEWCLZODPBN-JGWLITMVSA-N 0.000 abstract 1
- 239000003921 oil Substances 0.000 description 9
- 238000011081 inoculation Methods 0.000 description 5
- 230000036039 immunity Effects 0.000 description 4
- 238000010255 intramuscular injection Methods 0.000 description 4
- 239000007927 intramuscular injection Substances 0.000 description 4
- 238000004945 emulsification Methods 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 230000036528 appetite Effects 0.000 description 2
- 235000019789 appetite Nutrition 0.000 description 2
- 230000036760 body temperature Effects 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000001900 immune effect Effects 0.000 description 2
- 230000003053 immunization Effects 0.000 description 2
- 235000019271 petrolatum Nutrition 0.000 description 2
- 206010067484 Adverse reaction Diseases 0.000 description 1
- 241000700199 Cavia porcellus Species 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 241000283898 Ovis Species 0.000 description 1
- 239000004264 Petrolatum Substances 0.000 description 1
- 241000700605 Viruses Species 0.000 description 1
- 230000006838 adverse reaction Effects 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 230000007815 allergy Effects 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 230000005875 antibody response Effects 0.000 description 1
- FUSUHKVFWTUUBE-UHFFFAOYSA-N buten-2-one Chemical group CC(=O)C=C FUSUHKVFWTUUBE-UHFFFAOYSA-N 0.000 description 1
- 206010061428 decreased appetite Diseases 0.000 description 1
- 239000002552 dosage form Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229940066842 petrolatum Drugs 0.000 description 1
- 229920000136 polysorbate Polymers 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Abstract
The invention relates to an oil emulsion inactivated vaccine for parvoviral diseases of pigs, which is prepared by charging deactivated porcine parvovirus (PPV) into oil adjuvant for emulsion, wherein the oil adjuvant is prepared through charging aluminum tristearate into the stable solution of 94 wt% white oil and 6 wt% sorbitan mono-oleate-80, the charging amount being 2g of aluminum tristearate into each 100ml of the stable solution.
Description
Technical field:
The invention belongs to the biological new medicine technical field of veterinary, particularly a kind of Inactive Oil-emulsion Porcine Parvovirus Vaccine.
Background technology:
Original pig parvoviral AEI (acetyl ethylene margin amine) inactivated vaccine is the pig parvoviral of deactivation (PPV) to be added oily adjuvant through the formulated two-phase oil emulsion vaccine of emulsifying, oily adjuvant prescription (petrolatum: lanoline=9: 1).This immune effect of vaccine is good, but has the following disadvantages and problem:
1, the less stable of vaccine, layering are fast, and the Emulsion Seedling is divided into two layers of milky and pale reds very soon after preserving, and can fold a small amount of oil reservoir on emulsion face upper strata, and the outward appearance physical behavior is not good enough, and breakdown of emulsion takes place in the shelf-life sometimes, has influenced vaccine quality.Main cause wherein is the problem of oily adjuvant.
2, occur inappetence sometimes after the pig body vaccinate, spirit is not good enough, have also can generate heat, untoward reaction such as allergy, inoculation position is obviously red and swollen.The main cause that causes is relevant with oily adjuvant, and is also relevant greatly with the dosage of vaccine use.
3, the dosage of this vaccine use is that 1 pig will be injected 2 times, each 5 milliliters, and two weeks of interval, 10 milliliters of accumulated doses.For this reason, the user is reflected the formality trouble that vaccine uses, and increases the labor intensity of epidemic prevention.
Summary of the invention:
The invention provides the pig parvoviral AEI inactivated vaccine behind a kind of optimize,, solve technical problems such as fast and shelf-life of less stable, the layering of existing product vaccine, and reduce the using dosage of vaccine by changing the prescription of oily adjuvant.
For solving the problems of the technologies described above, the present invention is achieved in that
A kind of Inactive Oil-emulsion Porcine Parvovirus Vaccine, be that the oily adjuvant of pig parvoviral (PPV) adding of deactivation is formulated through emulsifying, it is characterized in that: oily adjuvant is to add aluminium stearate in the liquid according to percentage by weight 94% white oil and surely closing of 6% Si Ben-80 composition, and its addition is 100 milliliters and surely closes liquid adding 2 gram aluminium stearate.
Adopt one-step method emulsifying.
Adopt two step method emulsifying.
The present invention has following technique effect:
1, improve the physical behavior of vaccine, improve vaccine quality, using instead the oily adjuvant (being made up of 9 parts of petrolatums and 1 part of Adeps caprae seu ovis) that uses originally by white oil is the main oily adjuvant of forming.Through repeatedly comparing, (outward appearance, viscosity, stability, dosage form etc.) have evident difference aspect physical behavior, also are like this even adopt different emulsification methods to the vaccine of these two kinds oily adjuvants preparations.No matter adopting one-step method emulsifying with new oily adjuvant still is two step method emulsifying, vaccine was placed 1-2 days at normal temperatures, very a spot of pale red emulsion only appears in the bottle end of 5000 milliliters of vaccine loading amounts, vaccine stability with old oily adjuvant preparation is poor, layering is fast, and the vaccine for preparing just can be divided into two layers soon.
2, the dosage of Shi Yonging reduces: the usage and dosage of original vaccine is intramuscular injection 2 times, each 5 milliliters, and two weeks of interval; The usage and dosage of optimizing the vaccine of vaccine is 2 milliliters of 1 intramuscular injection, 2 milliliters of 1 immunity.
The specific embodiment:
A kind of Inactive Oil-emulsion Porcine Parvovirus Vaccine, be that the pig parvoviral (PPV) of deactivation is added oily adjuvant is to add aluminium stearate in the liquid surely closing of forming according to percentage by weight 94% white oil and 6% Si Ben-80 through the formulated oily adjuvant of emulsifying, its addition is 100 milliliters and surely closes liquid and add 2 gram aluminium stearate.
The amount that contains aluminium stearate in the oil adjuvant is relevant with the stability of vaccine, carrying out repeatedly emulsification test proves, when surely closing of 100 milliliters of white oils and Si Ben-80 composition adds the 1-1.5 gram in the liquid, vaccine is through 3000r.p.m after centrifugal 15 minutes, can divide two layers, the upper strata milky, lower floor's pale red capacity accounts for 2/5-3/5; When adding 2 grams, through two layers of 15 minutes centrifugal backs of 3000r.p.m branches, lower floor accounts for 1/5-1/6; When adding 4 grams, the viscosity of vaccine obviously increases, and a spot of white precipitate can occur.
Adopt new oily adjuvant preparation vaccine, make the outward appearance of vaccine and physical behavior be improved significantly, the oily adjuvant of forming with white oil, Si Ben-80 and aluminium stearate is an oil phase, adding 2% tween in the virus is water, can make viscosity＜2 by two-step emulsification method "; stability is better preserved the not two-phase of breakdown of emulsion (W/O/W) oil emulsion inactivated vaccine at 4 ℃.Thereby the method and the standard of check vaccine physical behavior have been set up.
Inactive Oil-emulsion Porcine Parvovirus Vaccine inoculation 2-3 age in days neonatal rat through optimizing, safety is passed through.The back all produces antibody response, HI:128～1024 around the inoculation Cavia porcellus.Inoculating 6 batches of responsive pigs does not have obvious adverse reaction, and body temperature, appetite are normal, and inoculation portion does not have obvious red and swollen piece.
The usage and dosage of original vaccine is intramuscular injection 2 times, each 5 milliliters, and two weeks of interval.
The usage and dosage of optimizing the vaccine of vaccine is 2 milliliters of 1 intramuscular injection, 2 milliliters 1 time.
Concrete result of use of the present invention sees also following table:
| The vaccine title | Type | Immunizing dose | Immune time | Use | Safety * | Physical behavior | Immunity | Storage life | Duration of immunity |
| Inactive Oil-emulsion Porcine Parvovirus Vaccine | Optimize the back vaccine | 2ml/ time | 1 time | Convenient | Better | Better | ??94％ | At least 7 months | 6 months |
| Pig parvoviral AEI inactivated vaccine | Original vaccine (optimize) | 5ml/ time | 2 times | Trouble | Weak point is arranged | Not good enough | ??94.1％ | At least 7 months | 6 months |
*Safety: referring to have denys non-specific untoward reaction: inoculation position has or not obvious red and swollen piece, and whether appetite, spirit, body temperature is normal.
In sum, through transform and optimize after Inactive Oil-emulsion Porcine Parvovirus Vaccine not only obviously be better than original vaccine in the stability and the safety of outward appearance and physical behavior, vaccine, and immunizing dose reduces 5 times, immune time is kept to 1 time, the immune effect of vaccine is certain, every immunity index is constant, thereby greatly reduces cost, has made things convenient for the user.
Claims (3)
1, a kind of Inactive Oil-emulsion Porcine Parvovirus Vaccine, be that the oily adjuvant of pig parvoviral (PPV) adding of deactivation is formulated through emulsifying, it is characterized in that: oily adjuvant is to add aluminium stearate in the liquid according to percentage by weight 94% white oil and surely closing of 6% Si Ben-80 composition, and its addition is 100 milliliters and surely closes liquid adding 2 gram aluminium stearate.
2, Inactive Oil-emulsion Porcine Parvovirus Vaccine according to claim 1 is characterized in that: adopt one-step method emulsifying.
3, Inactive Oil-emulsion Porcine Parvovirus Vaccine according to claim 1 is characterized in that: adopt two step method emulsifying.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410024754 CN1704119A (en) | 2004-05-28 | 2004-05-28 | Oil emulsion inactivated vaccine for parvoviral diseases of pigs |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410024754 CN1704119A (en) | 2004-05-28 | 2004-05-28 | Oil emulsion inactivated vaccine for parvoviral diseases of pigs |
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| Publication Number | Publication Date |
|---|---|
| CN1704119A true CN1704119A (en) | 2005-12-07 |
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|---|---|---|---|
| CN 200410024754 Pending CN1704119A (en) | 2004-05-28 | 2004-05-28 | Oil emulsion inactivated vaccine for parvoviral diseases of pigs |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102205120A (en) * | 2011-05-20 | 2011-10-05 | 青岛易邦生物工程有限公司 | Production method of porcine parvovirus inactivated vaccine |
| CN101474403B (en) * | 2009-01-23 | 2012-02-08 | 南京联海化工有限公司 | oil adjuvant of inactivated vaccine |
| CN101851609B (en) * | 2010-02-02 | 2012-10-03 | 哈药集团生物疫苗有限公司 | Porcine parvovirus L strain and use thereof in preparation of porcine parvovirus inactivated vaccines |
| CN102719407A (en) * | 2012-05-25 | 2012-10-10 | 中国农业科学院哈尔滨兽医研究所 | Porcine parvovirus BQ-C strain and application of porcine parvovirus BQ-C strain to preparation of inactivated porcine parvovirus vaccine |
| CN102965345A (en) * | 2012-11-19 | 2013-03-13 | 江苏省农业科学院 | Porcine parvovirus, vaccine composition and application thereof |
| CN107522775A (en) * | 2009-08-21 | 2017-12-29 | 弗吉尼亚理工大学知识产权公司 | The vaccine of the thin circovirus virus of pig and diagnosis |
-
2004
- 2004-05-28 CN CN 200410024754 patent/CN1704119A/en active Pending
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101474403B (en) * | 2009-01-23 | 2012-02-08 | 南京联海化工有限公司 | oil adjuvant of inactivated vaccine |
| CN107522775A (en) * | 2009-08-21 | 2017-12-29 | 弗吉尼亚理工大学知识产权公司 | The vaccine of the thin circovirus virus of pig and diagnosis |
| CN101851609B (en) * | 2010-02-02 | 2012-10-03 | 哈药集团生物疫苗有限公司 | Porcine parvovirus L strain and use thereof in preparation of porcine parvovirus inactivated vaccines |
| CN102205120A (en) * | 2011-05-20 | 2011-10-05 | 青岛易邦生物工程有限公司 | Production method of porcine parvovirus inactivated vaccine |
| CN102719407A (en) * | 2012-05-25 | 2012-10-10 | 中国农业科学院哈尔滨兽医研究所 | Porcine parvovirus BQ-C strain and application of porcine parvovirus BQ-C strain to preparation of inactivated porcine parvovirus vaccine |
| CN102719407B (en) * | 2012-05-25 | 2013-09-04 | 中国农业科学院哈尔滨兽医研究所 | Porcine parvovirus BQ-C strain and application of porcine parvovirus BQ-C strain in preparation of inactivated porcine parvovirus vaccine |
| CN102965345A (en) * | 2012-11-19 | 2013-03-13 | 江苏省农业科学院 | Porcine parvovirus, vaccine composition and application thereof |
| CN102965345B (en) * | 2012-11-19 | 2014-04-30 | 江苏省农业科学院 | Porcine parvovirus, vaccine composition and application thereof |
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