CN1660419A - Urease composite urea nitrogen adsorbent and preparation method thereof - Google Patents
Urease composite urea nitrogen adsorbent and preparation method thereof Download PDFInfo
- Publication number
- CN1660419A CN1660419A CN 200410093894 CN200410093894A CN1660419A CN 1660419 A CN1660419 A CN 1660419A CN 200410093894 CN200410093894 CN 200410093894 CN 200410093894 A CN200410093894 A CN 200410093894A CN 1660419 A CN1660419 A CN 1660419A
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- China
- Prior art keywords
- urase
- urea nitrogen
- cellulose
- dialdehyde
- immobilized urease
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 239000003463 adsorbent Substances 0.000 title claims abstract description 25
- 108010046334 Urease Proteins 0.000 title claims abstract description 24
- PNNCWTXUWKENPE-UHFFFAOYSA-N [N].NC(N)=O Chemical compound [N].NC(N)=O PNNCWTXUWKENPE-UHFFFAOYSA-N 0.000 title claims abstract description 20
- 238000002360 preparation method Methods 0.000 title claims description 7
- 239000002131 composite material Substances 0.000 title 1
- 229920002678 cellulose Polymers 0.000 claims abstract description 9
- 239000001913 cellulose Substances 0.000 claims abstract description 9
- 238000001035 drying Methods 0.000 claims abstract description 9
- 238000003756 stirring Methods 0.000 claims abstract description 9
- 108010010803 Gelatin Proteins 0.000 claims abstract description 8
- 229920000159 gelatin Polymers 0.000 claims abstract description 8
- 239000008273 gelatin Substances 0.000 claims abstract description 8
- 235000019322 gelatine Nutrition 0.000 claims abstract description 8
- 235000011852 gelatine desserts Nutrition 0.000 claims abstract description 8
- 229920001661 Chitosan Polymers 0.000 claims abstract description 6
- ZNZYKNKBJPZETN-WELNAUFTSA-N Dialdehyde 11678 Chemical compound N1C2=CC=CC=C2C2=C1[C@H](C[C@H](/C(=C/O)C(=O)OC)[C@@H](C=C)C=O)NCC2 ZNZYKNKBJPZETN-WELNAUFTSA-N 0.000 claims description 18
- 239000008280 blood Substances 0.000 claims description 18
- 210000004369 blood Anatomy 0.000 claims description 18
- 150000001875 compounds Chemical class 0.000 claims description 15
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims description 9
- 235000010980 cellulose Nutrition 0.000 claims description 7
- 229920000168 Microcrystalline cellulose Polymers 0.000 claims description 5
- 235000019813 microcrystalline cellulose Nutrition 0.000 claims description 5
- 239000008108 microcrystalline cellulose Substances 0.000 claims description 5
- 229940016286 microcrystalline cellulose Drugs 0.000 claims description 5
- 235000010489 acacia gum Nutrition 0.000 claims description 4
- 239000001785 acacia senegal l. willd gum Substances 0.000 claims description 4
- 238000000034 method Methods 0.000 claims description 2
- 239000000203 mixture Substances 0.000 claims description 2
- 230000000274 adsorptive effect Effects 0.000 abstract 1
- 239000011248 coating agent Substances 0.000 abstract 1
- 238000000576 coating method Methods 0.000 abstract 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 2
- 150000001299 aldehydes Chemical class 0.000 description 2
- 208000020832 chronic kidney disease Diseases 0.000 description 2
- 208000022831 chronic renal failure syndrome Diseases 0.000 description 2
- 238000001179 sorption measurement Methods 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 235000013877 carbamide Nutrition 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
Landscapes
- Enzymes And Modification Thereof (AREA)
- Medicinal Preparation (AREA)
Abstract
A urea nitrogen adsorbent is prepared from immobilized urease and dialdehydel cellulose through dissolving the urease in the solution of gelatin, chitosan or ababic gum, stirring, coating it on a smooth plate, drying to obtain immobilized urease, and proportionally mixing it with dialdehydyl cellulose. It has high adsorptive power.
Description
Technical field
The invention relates to compound blood urea nitrogen adsorbent of a kind of urase and preparation method thereof, belong to the adsorbent technology of blood urea nitrogen.
Background technology
Chronic renal failure (CRF) is that a kind of prognosis is not good, the disease of serious threat patient life.Uremic main poisonous substance is a carbamide, oral adsorbent is a kind of active and effective Therapeutic Method, its principle is, adsorbent under the clothes is behind the intestines and stomach of human body, absorption is accumulated in intravital uremic thing, excrete together in company with feces, alleviate uremic symptom, suppress the purpose that the state of an illness further worsens thereby can reach.
The present domestic oral adsorbent of producing blood urea nitrogen mainly is a Coated Aldehyde Oxystarch, and this oral adsorbent effective percentage reaches more than 80%, obtains the trust of extensive patients.But a little less than the absorbability of this adsorbent, be 0.38-0.41mgN/g at the 6-8h absorbability, unsatisfactory.
Summary of the invention
The object of the present invention is to provide compound blood urea nitrogen adsorbent of a kind of urase and preparation method thereof, this adsorbent is not only to the blood urea nitrogen high adsorption capacity, and its preparation technology is simple.
The present invention is realized by following technical proposals, the compound blood urea nitrogen adsorbent of a kind of urase, it is characterized in that, to be fixed in gelatin, chitosan or arabic gum, the urase vigor is the immobilized urease of 4.37-6.07U/g, with the microcrystalline Cellulose or the alpha-cellulose that contain dialdehyde-based quality 30%-95%, by mass ratio 1: 10-100 mixes.
The compound blood urea nitrogen adsorbent of above-mentioned urase preparation method is characterized in that comprising following process:
1. the 1.0g urase is dissolved in gelatin, chitosan or the arabic gum that mass concentration is 5-10%, in the 20-40mL solution, stirs, be coated on the slick flat board,, peel off, grind, make immobilized urease (IE) through natural drying.
By the urase quality in the immobilized urease (IE) and the cellulose quality that contains dialdehyde-based than 1: 10-100, the immobilized urease that step 1 is obtained mix with the microcrystalline Cellulose of dialdehyde-based mass content 30%-95% or alpha-cellulose and obtain the compound blood urea nitrogen adsorbent of urase.
The invention has the advantages that: high adsorption capacity is 20.0-30.0mgN/g at the 6-8h absorbability, and its absorbability is 50-100 a times of Coated Aldehyde Oxystarch, and in addition, adsorbent of the present invention has no side effect, and it is convenient to make raw material sources, low price, and technology is simple.
The specific embodiment
With embodiment the present invention is illustrated below.
Example 1
With the urase vigor be the 1.0g urase of 4.37U/g to be scattered in the 20mL mass concentration be in 10% the gelatin solution, be coated on after stirring on the slick plastic plate, natural drying is peeled off, and grinds, and makes 3g immobilized urease (IE).With 0.3g immobilized urease and 1.0g dialdehyde-based content be 95% dialdehyde-based microcrystalline Cellulose after mixed at room temperature, make the compound blood urea nitrogen adsorbent of 1.3g urase.
Example 2
With the urase vigor be the 1.0g urase of 4.37U/g to be scattered in the 40mL mass concentration be in 5% the gelatin solution, be coated on after stirring on the slick plastic plate, natural drying is peeled off, and grinds, and makes 3g immobilized urease (IE).With 0.3g immobilized urease and 5.0g dialdehyde-based content be 70% dialdehyde-based alpha-cellulose cellulose after mixed at room temperature, make the compound blood urea nitrogen adsorbent of 5.3g urase.
Example 3
With the urase vigor be the 1.0g urase of 4.97U/g to be scattered in the 25mL mass concentration be in 8% the gelatin solution, be coated on after stirring on the slick plastic plate, natural drying is peeled off, and grinds, and makes 3g immobilized urease (IE).With 0.3g immobilized urease and 8.0g dialdehyde-based content be 50% dialdehyde-based alpha-cellulose cellulose after mixed at room temperature, make the compound blood urea nitrogen adsorbent of 8.3g urase.
Example 4
Is in 6% the chitosan solution with the urase vigor for the 4.98U/g1.0g urase is scattered in the 33mL mass concentration, is coated on after stirring on the slick plastic plate, and natural drying is peeled off, and grinds, and makes 3g immobilized urease (IE).With 0.3g immobilized urease and 10.0g dialdehyde-based content be 60% dialdehyde-based alpha-cellulose cellulose after mixed at room temperature, make the compound blood urea nitrogen adsorbent of 10.3g urase.
Example 5
Is in 10% the gumwater with the urase vigor for the 5.05U/g1.0g urase is scattered in the 20mL mass concentration, is coated on after stirring on the slick plastic plate, and natural drying is peeled off, and grinds, and makes 3g immobilized urease (IE).With 0.3g immobilized urease and 5.0g dialdehyde-based content be 88% dialdehyde-based alpha-cellulose cellulose after mixed at room temperature, make the compound blood urea nitrogen adsorbent of 5.3g urase.
Example 6
Is in 5% the gumwater with the urase vigor for the 6.07U/g1.0g urase is scattered in the 40mL mass concentration, is coated on after stirring on the slick plastic plate, and natural drying is peeled off, and grinds, and makes 3g immobilized urease (IE).With 0.3g immobilized urease and 8.0g dialdehyde-based content be 88% dialdehyde-based alpha-cellulose cellulose after mixed at room temperature, make the compound blood urea nitrogen adsorbent of 8.3g urase.
Claims (2)
1. compound blood urea nitrogen adsorbent of urase, it is characterized in that, to be fixed in gelatin, chitosan or arabic gum, the urase vigor is the immobilized urease of 4.37-6.07U/g, with the microcrystalline Cellulose or the alpha-cellulose that contain dialdehyde-based quality 30%-95%, by mass ratio 1: 10-100 mixes.
2. one kind prepares the compound blood urea nitrogen adsorbent of right 1 described urase preparation method, it is characterized in that comprising following process:
1) the 1.0g urase is dissolved in the solution that mass concentration is 5-10%, 20-40mL gelatin, chitosan or arabic gum, stirs, be coated on the slick flat board,, peel off, grind, make immobilized urease through natural drying;
2) by the urase quality in the immobilized urease and the cellulose quality that contains dialdehyde-based than 1: 10-100, the immobilized urease that step 1) is obtained mix with the microcrystalline Cellulose of dialdehyde-based mass content 30%-95% or alpha-cellulose and obtain the compound blood urea nitrogen adsorbent of urase.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410093894 CN1660419A (en) | 2004-12-10 | 2004-12-10 | Urease composite urea nitrogen adsorbent and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 200410093894 CN1660419A (en) | 2004-12-10 | 2004-12-10 | Urease composite urea nitrogen adsorbent and preparation method thereof |
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| Publication Number | Publication Date |
|---|---|
| CN1660419A true CN1660419A (en) | 2005-08-31 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 200410093894 Pending CN1660419A (en) | 2004-12-10 | 2004-12-10 | Urease composite urea nitrogen adsorbent and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1660419A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102732500A (en) * | 2012-07-04 | 2012-10-17 | 浙江农林大学 | Preparation method of oxidized dialdehyde cellulose immobilized urease |
| US8497107B2 (en) | 2008-09-30 | 2013-07-30 | Fresenius Medical Care Holdings, Inc. | Covalently immobilized enzyme and method to make the same |
| CN103228677A (en) * | 2010-12-09 | 2013-07-31 | 通用电气公司 | Cellulose substrates, compositions and methods for storage and analysis of biological materials |
-
2004
- 2004-12-10 CN CN 200410093894 patent/CN1660419A/en active Pending
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8497107B2 (en) | 2008-09-30 | 2013-07-30 | Fresenius Medical Care Holdings, Inc. | Covalently immobilized enzyme and method to make the same |
| US9187744B2 (en) | 2008-09-30 | 2015-11-17 | Fresenius Medical Care Holdings, Inc. | Covalently immobilized enzyme and method to make the same |
| CN103228677A (en) * | 2010-12-09 | 2013-07-31 | 通用电气公司 | Cellulose substrates, compositions and methods for storage and analysis of biological materials |
| CN102732500A (en) * | 2012-07-04 | 2012-10-17 | 浙江农林大学 | Preparation method of oxidized dialdehyde cellulose immobilized urease |
| CN102732500B (en) * | 2012-07-04 | 2013-09-04 | 浙江农林大学 | Preparation method of oxidized dialdehyde cellulose immobilized urease |
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