CN1375499A - Method of extracting and separating several components from mangsteen - Google Patents
Method of extracting and separating several components from mangsteen Download PDFInfo
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- CN1375499A CN1375499A CN02113548A CN02113548A CN1375499A CN 1375499 A CN1375499 A CN 1375499A CN 02113548 A CN02113548 A CN 02113548A CN 02113548 A CN02113548 A CN 02113548A CN 1375499 A CN1375499 A CN 1375499A
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- water
- fatty alcohol
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- momordica
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- 238000000034 method Methods 0.000 title claims abstract description 44
- 235000009815 Momordica Nutrition 0.000 claims abstract description 37
- 241000218984 Momordica Species 0.000 claims abstract description 37
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 35
- 239000011347 resin Substances 0.000 claims abstract description 29
- 229920005989 resin Polymers 0.000 claims abstract description 29
- 229920006122 polyamide resin Polymers 0.000 claims abstract description 14
- 150000004676 glycans Chemical class 0.000 claims abstract description 11
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 11
- 239000005017 polysaccharide Substances 0.000 claims abstract description 11
- 238000005406 washing Methods 0.000 claims abstract description 7
- 150000002191 fatty alcohols Chemical class 0.000 claims description 39
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 26
- 239000007788 liquid Substances 0.000 claims description 24
- 239000000243 solution Substances 0.000 claims description 19
- 239000007864 aqueous solution Substances 0.000 claims description 10
- 229930003935 flavonoid Natural products 0.000 claims description 10
- 150000002215 flavonoids Chemical class 0.000 claims description 10
- 235000017173 flavonoids Nutrition 0.000 claims description 10
- 241001409321 Siraitia grosvenorii Species 0.000 claims description 9
- 235000011171 Thladiantha grosvenorii Nutrition 0.000 claims description 9
- 239000006166 lysate Substances 0.000 claims description 9
- 238000001035 drying Methods 0.000 claims description 8
- 150000001450 anions Chemical class 0.000 claims description 7
- 239000012141 concentrate Substances 0.000 claims description 7
- 238000004090 dissolution Methods 0.000 claims description 7
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 6
- 239000003085 diluting agent Substances 0.000 claims description 6
- 239000011259 mixed solution Substances 0.000 claims description 6
- 238000000926 separation method Methods 0.000 claims description 6
- 239000007787 solid Substances 0.000 claims description 6
- -1 styrene series anion Chemical class 0.000 claims description 6
- 239000008399 tap water Substances 0.000 claims description 6
- 235000020679 tap water Nutrition 0.000 claims description 6
- 238000004821 distillation Methods 0.000 claims description 5
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 4
- 239000012153 distilled water Substances 0.000 claims description 4
- 238000013467 fragmentation Methods 0.000 claims description 4
- 238000006062 fragmentation reaction Methods 0.000 claims description 4
- 238000001914 filtration Methods 0.000 claims description 3
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims description 2
- LRHPLDYGYMQRHN-UHFFFAOYSA-N N-Butanol Chemical compound CCCCO LRHPLDYGYMQRHN-UHFFFAOYSA-N 0.000 claims description 2
- 238000009835 boiling Methods 0.000 claims description 2
- 239000001569 carbon dioxide Substances 0.000 claims description 2
- 229910002092 carbon dioxide Inorganic materials 0.000 claims description 2
- 238000005119 centrifugation Methods 0.000 claims description 2
- 238000007865 diluting Methods 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims description 2
- 239000000725 suspension Substances 0.000 claims description 2
- 235000013399 edible fruits Nutrition 0.000 abstract description 7
- 239000000284 extract Substances 0.000 abstract description 3
- 229930182470 glycoside Natural products 0.000 abstract description 2
- 150000002338 glycosides Chemical class 0.000 abstract description 2
- DNIAPMSPPWPWGF-GSVOUGTGSA-N (R)-(-)-Propylene glycol Chemical compound C[C@@H](O)CO DNIAPMSPPWPWGF-GSVOUGTGSA-N 0.000 abstract 4
- 239000002244 precipitate Substances 0.000 abstract 2
- 239000003957 anion exchange resin Substances 0.000 abstract 1
- 238000000746 purification Methods 0.000 abstract 1
- 239000000203 mixture Substances 0.000 description 8
- 238000000605 extraction Methods 0.000 description 6
- 239000004615 ingredient Substances 0.000 description 6
- 239000000047 product Substances 0.000 description 5
- ILRCGYURZSFMEG-UHFFFAOYSA-N Salidroside Natural products OC1C(O)C(O)C(CO)OC1OCCC1=CC=C(O)C=C1 ILRCGYURZSFMEG-UHFFFAOYSA-N 0.000 description 3
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 229930003944 flavone Natural products 0.000 description 3
- 235000011949 flavones Nutrition 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 238000001556 precipitation Methods 0.000 description 3
- ILRCGYURZSFMEG-RQICVUQASA-N salidroside Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)OC1OCCC1=CC=C(O)C=C1 ILRCGYURZSFMEG-RQICVUQASA-N 0.000 description 3
- 238000003828 vacuum filtration Methods 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- PPBRXRYQALVLMV-UHFFFAOYSA-N Styrene Chemical compound C=CC1=CC=CC=C1 PPBRXRYQALVLMV-UHFFFAOYSA-N 0.000 description 2
- GAMYVSCDDLXAQW-AOIWZFSPSA-N Thermopsosid Natural products O(C)c1c(O)ccc(C=2Oc3c(c(O)cc(O[C@H]4[C@H](O)[C@@H](O)[C@H](O)[C@H](CO)O4)c3)C(=O)C=2)c1 GAMYVSCDDLXAQW-AOIWZFSPSA-N 0.000 description 2
- 235000009508 confectionery Nutrition 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 150000002212 flavone derivatives Chemical class 0.000 description 2
- 229930182478 glucoside Natural products 0.000 description 2
- 150000008131 glucosides Chemical class 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- BDERNNFJNOPAEC-UHFFFAOYSA-N propan-1-ol Chemical compound CCCO BDERNNFJNOPAEC-UHFFFAOYSA-N 0.000 description 2
- VHBFFQKBGNRLFZ-UHFFFAOYSA-N vitamin p Natural products O1C2=CC=CC=C2C(=O)C=C1C1=CC=CC=C1 VHBFFQKBGNRLFZ-UHFFFAOYSA-N 0.000 description 2
- 229920002307 Dextran Polymers 0.000 description 1
- 208000001953 Hypotension Diseases 0.000 description 1
- 206010061218 Inflammation Diseases 0.000 description 1
- 229920000057 Mannan Polymers 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000001476 alcoholic effect Effects 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 235000011114 ammonium hydroxide Nutrition 0.000 description 1
- 229910052799 carbon Inorganic materials 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 239000000470 constituent Substances 0.000 description 1
- 230000006378 damage Effects 0.000 description 1
- 238000010612 desalination reaction Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 150000002213 flavones Chemical class 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 235000021022 fresh fruits Nutrition 0.000 description 1
- 208000021822 hypotensive Diseases 0.000 description 1
- 230000001077 hypotensive effect Effects 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 239000002932 luster Substances 0.000 description 1
- LUEWUZLMQUOBSB-GFVSVBBRSA-N mannan Chemical class O[C@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@@H](O[C@@H]2[C@H](O[C@@H](O[C@H]3[C@H](O[C@@H](O)[C@@H](O)[C@H]3O)CO)[C@@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O LUEWUZLMQUOBSB-GFVSVBBRSA-N 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 239000002893 slag Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The method for extracting and separating several components from Grosvenor's momordica fruit includes the following steps: washing Grosvenor's momordica fruit with water, using alkaline water to treat broken Grosvenor's momordica fruit to obtain extract, then using aliphatic alcohol to make treatment to obtain precipitate and dissolved liquor, making the precipitate pass through anion-exchange resin column and undergo the processes of aliphatic alcohol treatment and purification to obtain the Grosvenor's momordica fruit polysaccharide, and making the dissolved liquor pass through the polyamide resin column and macroporous resin column and undergo the process of aliphatic alcohol treatment to obtain Grosvenor's momordica fruit glycoside, and making the component adsorbed by polyamide resin column undergo the processes of washing with basic aliphatic alcohol.
Description
(1) technical field:
The present invention relates to a kind of from Grosvenor Momordica the method for extraction separation multiple components such as momordica grosvenori polysaccharide grosvenor momordica flavonoid, Momordica-Glycosides.
(2) background technology:
Now, people just extract this effective ingredient of Momordica-Glycosides at large from Grosvenor Momordica, and the method for use generally is exactly to use flooding, and concentrate drying moisture content promptly obtains Momordica-Glycosides then.This method can only obtain a kind of effective ingredient in the Grosvenor Momordica, and the product composition complexity that obtains, and total salidroside content is not high.
(3) summary of the invention:
Purpose of the present invention just provides and a kind ofly can go out multiple effective ingredient by extraction separation from Grosvenor Momordica, and as the method for momordica grosvenori polysaccharide, grosvenor momordica flavonoid, Momordica-Glycosides, its composition of the product of this method gained is single relatively, and mainly contains the content height of effective constituent.
The objective of the invention is to realize by following step.
1) handle broken Grosvenor Momordica with buck again after the water,
2) separating suspension and merge two kinds of extracting solutions,
3) handle extracting solution with Fatty Alcohol(C12-C14 and C12-C18),
4) separation obtains throw out (I) and lysate (II),
5) use the resin anion(R.A) post to handle the water-soluble liquid of throw out (I),
6) it is effusive and add the water dissolution thing of washing out (III) to collect the resin anion(R.A) post,
7), and isolate the solid momordica grosvenori polysaccharide with Fatty Alcohol(C12-C14 and C12-C18) treating water solute (III),
8) Fatty Alcohol(C12-C14 and C12-C18) in the lysate (II) is removed in distillation, the residue dilute with water,
9) water diluent is flow through polyamide resin column,
10) with the effluent liquid in the macroporous resin column processing polyamide resin column,
11) collect the effluent liquid and the elutriant of concentration fatty alcohol aqueous solution wash-out from low to high from macroporous resin column,
12) Fatty Alcohol(C12-C14 and C12-C18) is handled the mixed solution of collecting liquid from macroporous resin column, and isolates insolubles,
13) isolate that concentrate drying goes out Momordica-Glycosides in the liquid of insolubles,
14) polyamide resin column behind the alkaline fat alcohol scrub stream solid carbon dioxide diluent,
15) concentrate drying goes out grosvenor momordica flavonoid in the washings of polyamide resin column.
Method of the present invention can be handled the Grosvenor Momordica fresh fruit, also can handle the Grosvenor Momordica dry fruit, and Grosvenor Momordica helps the abundant stripping of various compositions after fragmentation, and it is more suitable generally it to be broken into the about 1～2cm of diameter.Earlier with tap water the Grosvenor Momordica of fragmentation is boiled 30～40min, isolated suspended substance is that slag boils 30～40min with the buck of PH=8.5～9.5 again.The tap water consumption is the broken Grosvenor Momordica of 8～12L/kg, and the buck consumption is the broken Grosvenor Momordica of 6～10L/kg.
In the method for the present invention, Fatty Alcohol(C12-C14 and C12-C18) is selected from methyl alcohol, ethanol, propyl alcohol, Virahol and propyl carbinol.As select ethanol for use, all suit from considerations such as price, security, sources.When handling extracting solution with Fatty Alcohol(C12-C14 and C12-C18), the suitable consumption of Fatty Alcohol(C12-C14 and C12-C18) is volume ratio 5: 1～6: 1 extracting solutions.Extracting solution elder generation after concentrating a large amount of moisture content of removal, is added Fatty Alcohol(C12-C14 and C12-C18) again, also just significantly reduced total consumption of Fatty Alcohol(C12-C14 and C12-C18).
In order to separate in the method for the present invention the mixture of lysate that some step produces and throw out or solid, insolubles, use filtration, centrifugation or settlement separate method.
Resin anion(R.A) has the effect of decolouring, desalination, decon, and method of the present invention is selected 201 * 7 styrene series anion resins, 202 * 7 styrene series anion resins for use, is the best with 201 * 7 styrene series anion resins.
Use the tap water except that beginning most to handle water and the buck that broken Grosvenor Momordica uses, all the other operations are because of all belonging to purifying character, so all adopt distilled water.
Effusive and add the water dissolution thing (III) washed out in case of necessity and preferably remove a large amount of moisture content through concentrating earlier from the resin anion(R.A) post, handle with Fatty Alcohol(C12-C14 and C12-C18) again, no matter whether concentrate, 9: 1 by volume～11: 1 water dissolution things (III) of the consumption of Fatty Alcohol(C12-C14 and C12-C18) or its enriched material, thus also as can be known, after concentrating, can significantly reduce total consumption of Fatty Alcohol(C12-C14 and C12-C18) earlier.
In the method for the present invention, the method for removing Fatty Alcohol(C12-C14 and C12-C18) generally adopts distillation method, preferably underpressure distillation.Behind the Fatty Alcohol(C12-C14 and C12-C18) in having removed lysate (II), the summation water yield of existing water is the Grosvenor Momordica of 8～12L/kg fragmentation in the water of dilution residue and the residue.
It is best with D101 that the macroporous resin that method of the present invention is used is selected from D101, D102, AB-8.With the effective ingredient of the fatty alcohol aqueous solution wash-out macroporous resin adsorption of concentration from low to high, mainly be if directly use the high density fatty alcohol aqueous solution, to make some effective ingredient calmness in post, can not wash out, utilize the variation of concentration from low to high, just can be successively effective ingredient (sweet glucoside) order of various molecular weight be washed out, make the yield of total sweet glucoside and content all effectively improve.The concentration of lower concentration fatty alcohol aqueous solution is minimum to be volumetric concentration 40%, and the maximum concentration of high density fatty alcohol aqueous solution is a volumetric concentration 95%.The mixed solution of the gleanings that obtains from macroporous resin column is preferably removed Fatty Alcohol(C12-C14 and C12-C18) and a large amount of moisture content through concentrating earlier, adds Fatty Alcohol(C12-C14 and C12-C18) again and handles.Certainly also can directly add Fatty Alcohol(C12-C14 and C12-C18) in mixed solution handles.No matter whether concentrate, it is volume content 70～80% that the Fatty Alcohol(C12-C14 and C12-C18) of adding makes Fatty Alcohol(C12-C14 and C12-C18) total content therein.
Method of the present invention uses alkaline fat alcohol to wash out another kind of effective ingredient---the grosvenor momordica flavonoid of polyamide resin absorption from the polyamide resin column behind the water diluent that drains off.The pH value of alkaline fat alcohol is controlled to be 8.5～9.5, and alkaline fat alcohol is for containing the aqueous solution of 65～75% volume Fatty Alcohol(C12-C14 and C12-C18).
For the natural flavour mountaineous and color and luster that keeps various extraction compositions and prevent destruction in the course of processing and rotten, the drying temperature of the various extraction compositions of regulation all is lower than 85 ℃ in the method for the present invention.
By method of the present invention, extraction separation goes out three kinds of compositions from Grosvenor Momordica, and they are momordica grosvenori polysaccharide, Momordica-Glycosides and grosvenor momordica flavonoid, and total salidroside content 〉=80% of Momordica-Glycosides, than 70～75% height of known method.Momordica grosvenori polysaccharide mainly is the polysaccharide material of dextran and mannans.The grosvenor momordica flavonoid that the present invention extracts contains total flavones 〉=15%.The measuring method of general glycoside, total reducing sugar and general flavone content is a method in common.Three kinds are extracted all suitable healthcare products and the foodstuff additive done of composition.Momordica-Glycosides is good sweeting agent, and momordica grosvenori polysaccharide has effect hypotensive and that improve immunizing power, and grosvenor momordica flavonoid has the anti-inflammation and sterilization effect.
(4) embodiment:
The following examples describe the present invention in detail.
Get Grosvenor Momordica dry fruit 100g, be broken into the fragment of the about 1～2cm of diameter.With 1000ml boiling tap water 35min, after the filtration of 60 eye mesh screens, filter residue uses 800ml buck (transferring PH=9 with NaOH) to boil 35min again, filters with 60 eye mesh screens earlier, and merging two kinds of filtrates must about 1800ml extracting solution.In temperature is 60 ℃ of left and right sides concentrating under reduced pressure extracting solutions, makes 1/20 (about 90ml) to original volume.With 500ml volumetric concentration 80% ethanol and the abundant mixing of concentrated solution, left standstill 1 hour, let alone precipitation, use the filter paper vacuum filtration afterwards, obtain throw out (I) and lysate (II).
Throw out (I) is used the 500ml dissolved in distilled water, pour glass column (25 millimeters of the diameters that 100g201 * 7 styrene series anion resins are housed into after leaching insoluble impurity, high 300 millimeters) in, after treating the capital absence of liquid, add 100ml distillation washing post, collect whole effluent liquid, must about 600ml water dissolution thing (III).In temperature is 60 ℃ of left and right sides concentrating under reduced pressure water-soluble liquids (III), makes 1/20 (about 30ml) to original volume.With 300ml volumetric concentration 95% ethanol and the abundant mixing of concentrated solution, leave standstill 1h, let alone precipitation, obtain throw out with the filter paper vacuum filtration afterwards, with its drying under 80 ℃ of temperature, obtain the momordica grosvenori polysaccharide product of 0.8g total sugar content 〉=30%.
1/20 (the about 30ml) that lysate (II) is evaporated to original volume about 60 ℃ is to remove ethanol and portion water, then with the dissolving of 1000ml distilled water diluting, diluent is poured glass column (25 millimeters of the diameters that the 100g polyamide resin is housed into, high 300 millimeters) in, appoint liquid all to drain off, collect the whole effluent liquid from post.Glass column (25 millimeters of the diameters of 100gD101 styrene type interpolymer macroporous resin in all pouring into again, effluent liquid are housed, high 300 millimeters) in, after treating the capital absence of liquid, add volumetric concentration 50% aqueous ethanolic solution 300ml, after treating the capital absence of liquid again, add volumetric concentration 90% aqueous ethanolic solution 200ml, can be washed till that effluent liquid does not have sweet taste under the macroporous resin column.Collect all effluent liquid from macroporous resin column and mixing it (about 1400ml), about 60 ℃, be evaporated to 1/20 (about 70ml) of original volume, to remove ethanol and most of water, it is volume content 77% that the aqueous ethanolic solution that adds 300ml volumetric concentration 95% then makes the alcoholic acid total content, fully stir evenly, the quiet 1h of putting lets alone precipitation.Isolate insolubles with the filter paper vacuum filtration, filtrate (about 370ml) is evaporated to original volume 1/20 (about 18ml) to remove ethanol and most of water about 60 ℃.Concentrated solution (about 18ml) is dry under 80 ℃ of temperature, obtains the Momordica-Glycosides product of the total salidroside content of 1.2g 〉=80%.
With the volume content of 500ml, PH=9 or the aqueous ethanolic solution (regulating pH value) of volumetric concentration 70% with ammoniacal liquor, to pour in the polyamide resin column after draining off, effusive effluent liquid at the bottom of the collection post drains off until pillar again.All effluent liquid (about 500ml) is evaporated to 1/20 (about 25ml) of original volume to remove ethanol, ammonia and water about 60 ℃.Concentrated solution (about 25ml) is dry under 80 ℃ of temperature, obtains the grosvenor momordica flavonoid of 0.1g general flavone content 〉=15%.
Claims (19)
1, a kind of method of extracting multiple components from Grosvenor Momordica is characterized in that:
1) handle broken Grosvenor Momordica with buck again after the water,
2) separating suspension and merge two kinds of extracting solutions,
3) handle extracting solution with Fatty Alcohol(C12-C14 and C12-C18),
4) separation obtains throw out (I) and lysate (II),
5) use the resin anion(R.A) post to handle the water-soluble liquid of throw out (I),
6) it is effusive and add the water dissolution thing of washing out (III) to collect the resin anion(R.A) post,
7), and isolate the solid momordica grosvenori polysaccharide with Fatty Alcohol(C12-C14 and C12-C18) treating water solute (III),
8) Fatty Alcohol(C12-C14 and C12-C18) in the lysate (II) is removed in distillation, the residue dilute with water,
9) water diluent is flow through polyamide resin column,
10) with the effluent liquid in the macroporous resin column processing polyamide resin column,
11) collect the effluent liquid and the elutriant of concentration fatty alcohol aqueous solution wash-out from low to high from macroporous resin column,
12) Fatty Alcohol(C12-C14 and C12-C18) is handled the mixed solution of collecting liquid from macroporous resin column, and isolates insolubles,
13) isolate that concentrate drying goes out Momordica-Glycosides in the liquid of insolubles,
14) polyamide resin column behind the alkaline fat alcohol scrub stream solid carbon dioxide diluent,
15) concentrate drying goes out grosvenor momordica flavonoid in the washings of polyamide resin column.
2, according to the method for claim 1, it is characterized in that after the water be boiling tap water 30～40min with the buck treating processes again, isolated suspended substance boils 30～40min with buck again.
3, according to the method for claim 1 or 2, it is characterized in that the water yield is the broken Grosvenor Momordica of 8～12L/kg, the buck amount is the broken Grosvenor Momordica of 6～10L/kg.
4,, it is characterized in that PH=8.5～9.5 of buck according to the method for claim 1 or 2.
5,, it is characterized in that Fatty Alcohol(C12-C14 and C12-C18) is selected from methyl alcohol, ethanol, Virahol and propyl carbinol according to the method for claim 1.
6, according to the method for claim 1, the volume ratio that it is characterized in that Fatty Alcohol(C12-C14 and C12-C18) and extracting solution is 5: 1～6: 1.
7, according to the method for claim 1, its spy is with filtration, centrifugation or settlement separate lysate and throw out or solid or insolubles.
8,, it is characterized in that resin anion(R.A) chooses from 201 * 7 styrene series anion resins, 202 * 7 styrene series anion resins according to the method for claim 1.
9, according to the method for claim 1, it is characterized in that water and the buck used except that the Grosvenor Momordica of handling fragmentation use the tap water, the water of all the other operations all is a distilled water.
10,, it is characterized in that the volume ratio of Fatty Alcohol(C12-C14 and C12-C18) and water dissolution thing (III) is 9: 1～11: 1 according to the method for claim 1.
11, according to the method for claim 1, the broken Grosvenor Momordica of 8～12L/kg that the Total Water after it is characterized in that diluting is.
12,, it is characterized in that macroporous resin is selected from D101, D102, AB-8 according to the method for claim 1.
13,, it is characterized in that the concentration of lower concentration fatty alcohol aqueous solution is minimum and be volumetric concentration 30% that the concentration of high density fatty alcohol aqueous solution is up to volumetric concentration 95% according to the method for claim 1.
14,, it is characterized in that being adjusted to Fatty Alcohol(C12-C14 and C12-C18) total content therein with Fatty Alcohol(C12-C14 and C12-C18) will be collected liquid from macroporous resin column mixed solution is volume content 70%～80% according to the method for claim 1.
15,, it is characterized in that the mixed solution of collection liquid in extracting solution, water dissolution thing (III) or the resin column is all handled with Fatty Alcohol(C12-C14 and C12-C18) earlier again after concentrating according to the method for claim 1.
16,, it is characterized in that PH=8.5～9.5 of alkaline fat alcohol according to the method for claim 1.
17,, it is characterized in that alkaline fat alcohol is for containing the aqueous solution of volume 65～75% Fatty Alcohol(C12-C14 and C12-C18) according to the method for claim 1 or 16.
18,, it is characterized in that drying temperature is lower than 85 ℃ according to the method for claim 1.
19,, it is characterized in that it being momordica grosvenori polysaccharide or grosvenor momordica flavonoid or Momordica-Glycosides according to the product of the arbitrary method gained of claim 1～18.
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| CN02113548A CN1375499A (en) | 2002-03-28 | 2002-03-28 | Method of extracting and separating several components from mangsteen |
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| CN02113548A CN1375499A (en) | 2002-03-28 | 2002-03-28 | Method of extracting and separating several components from mangsteen |
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| CN1375499A true CN1375499A (en) | 2002-10-23 |
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Cited By (21)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1293089C (en) * | 2004-06-04 | 2007-01-03 | 何伟平 | Low calorie candy made from luohanguo and its production |
| CN1308341C (en) * | 2005-02-05 | 2007-04-04 | 莫家群 | Method and device for extracting fresh momordica glycoside |
| CN1309839C (en) * | 2004-06-11 | 2007-04-11 | 桂林商源植物制品有限公司 | Triterpenoid saponin compound extracting process and orally taken product therewith |
| CN1313457C (en) * | 2003-10-28 | 2007-05-02 | 广西师范大学 | Grosvenor momordical flavone and its preparing prcess |
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| CN100382723C (en) * | 2003-09-22 | 2008-04-23 | 桂林思特新技术公司 | Fruit drink prepared from fruit of Grosvenor momordica |
| CN102742801A (en) * | 2012-07-18 | 2012-10-24 | 何超文 | Siraitia grosvenorii high dietary cellulose fruit powder and preparation method thereof |
| CN102977165A (en) * | 2012-12-07 | 2013-03-20 | 上海交通大学 | Optimized purification preparation method for momordica grosvenori flavine |
| CN101522058B (en) * | 2006-09-07 | 2014-01-29 | 维多利亚生物有限公司 | Sweetening compositions and methods of making them |
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| CN106317158A (en) * | 2016-07-29 | 2017-01-11 | 桂林实力科技有限公司 | Mogroside V extraction method |
| CN106333206A (en) * | 2016-08-26 | 2017-01-18 | 桂林莱茵生物科技股份有限公司 | Method for preparing momordica grosvenor juice with honey taste |
| CN106860503A (en) * | 2017-03-29 | 2017-06-20 | 桂林实力科技有限公司 | The method that flavones is extracted from Luohanguo's residue |
| CN106866759A (en) * | 2017-03-28 | 2017-06-20 | 广西甙元植物制品有限公司 | The technique that grosvenor momordica flavonoid is produced from the waste liquid of Momordica-Glycosides decolorizing resin post discharge |
| CN108516996A (en) * | 2018-05-24 | 2018-09-11 | 湖南华诚生物资源股份有限公司 | The extracting method of Siraitia grosvenorii prebiotics and the method for extracting momordica glycoside V simultaneously |
| CN110776580A (en) * | 2019-11-19 | 2020-02-11 | 湖南华诚生物资源股份有限公司 | Method for recovering high-content acidic polysaccharide from waste liquid flowing out of momordica grosvenori column |
| CN111297936A (en) * | 2020-02-24 | 2020-06-19 | 湖南华诚生物资源股份有限公司 | A method for extracting and separating total flavonoids, total triterpenoid saponins and total polysaccharides from Luo Han Guo root |
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2002
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| CN100382723C (en) * | 2003-09-22 | 2008-04-23 | 桂林思特新技术公司 | Fruit drink prepared from fruit of Grosvenor momordica |
| CN1313457C (en) * | 2003-10-28 | 2007-05-02 | 广西师范大学 | Grosvenor momordical flavone and its preparing prcess |
| CN1293089C (en) * | 2004-06-04 | 2007-01-03 | 何伟平 | Low calorie candy made from luohanguo and its production |
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| CN1308341C (en) * | 2005-02-05 | 2007-04-04 | 莫家群 | Method and device for extracting fresh momordica glycoside |
| CN1324043C (en) * | 2005-05-26 | 2007-07-04 | 广西中医学院 | Prepn and use of high-purity momordica glycoside V |
| US11464247B2 (en) | 2006-09-07 | 2022-10-11 | Guilin Gfs Monk Fruit Corp. | Sweetening compositions and processes for preparing them |
| CN101522058B (en) * | 2006-09-07 | 2014-01-29 | 维多利亚生物有限公司 | Sweetening compositions and methods of making them |
| CN102742801B (en) * | 2012-07-18 | 2013-07-24 | 何超文 | Siraitia grosvenorii high dietary cellulose fruit powder and preparation method thereof |
| CN102742801A (en) * | 2012-07-18 | 2012-10-24 | 何超文 | Siraitia grosvenorii high dietary cellulose fruit powder and preparation method thereof |
| CN102977165A (en) * | 2012-12-07 | 2013-03-20 | 上海交通大学 | Optimized purification preparation method for momordica grosvenori flavine |
| CN102977165B (en) * | 2012-12-07 | 2015-05-27 | 上海交通大学 | Optimized purification preparation method for momordica grosvenori flavine |
| CN106317158A (en) * | 2016-07-29 | 2017-01-11 | 桂林实力科技有限公司 | Mogroside V extraction method |
| CN106317158B (en) * | 2016-07-29 | 2018-01-12 | 桂林实力科技有限公司 | The extracting method of mogroside V |
| CN105996039A (en) * | 2016-08-03 | 2016-10-12 | 桂林实力科技有限公司 | Comprehensive utilization method of fructus momordicae |
| CN106333206A (en) * | 2016-08-26 | 2017-01-18 | 桂林莱茵生物科技股份有限公司 | Method for preparing momordica grosvenor juice with honey taste |
| US11576412B2 (en) | 2016-10-24 | 2023-02-14 | Guilin Gfs Monk Fruit Corporation | Extracts from fruits of the Cucurbitaceae family, and methods of preparing thereof |
| CN106866759A (en) * | 2017-03-28 | 2017-06-20 | 广西甙元植物制品有限公司 | The technique that grosvenor momordica flavonoid is produced from the waste liquid of Momordica-Glycosides decolorizing resin post discharge |
| CN106866759B (en) * | 2017-03-28 | 2019-09-10 | 湖南华诚生物资源股份有限公司 | The technique of grosvenor momordica flavonoid is produced from the waste liquid that Momordica-Glycosides decolorizing resin column is discharged |
| CN106860503A (en) * | 2017-03-29 | 2017-06-20 | 桂林实力科技有限公司 | The method that flavones is extracted from Luohanguo's residue |
| CN108516996B (en) * | 2018-05-24 | 2020-10-16 | 湖南华诚生物资源股份有限公司 | Method for extracting fructus momordicae prebiotics and method for simultaneously extracting mogroside V |
| CN108516996A (en) * | 2018-05-24 | 2018-09-11 | 湖南华诚生物资源股份有限公司 | The extracting method of Siraitia grosvenorii prebiotics and the method for extracting momordica glycoside V simultaneously |
| CN110776580A (en) * | 2019-11-19 | 2020-02-11 | 湖南华诚生物资源股份有限公司 | Method for recovering high-content acidic polysaccharide from waste liquid flowing out of momordica grosvenori column |
| CN111297936A (en) * | 2020-02-24 | 2020-06-19 | 湖南华诚生物资源股份有限公司 | A method for extracting and separating total flavonoids, total triterpenoid saponins and total polysaccharides from Luo Han Guo root |
| CN111297936B (en) * | 2020-02-24 | 2021-08-10 | 湖南华诚生物资源股份有限公司 | Method for extracting and separating total flavone, total triterpenoid saponin and total polysaccharide from momordica grosvenori roots |
| CN111764194A (en) * | 2020-07-02 | 2020-10-13 | 广西中烟工业有限责任公司 | A kind of preparation method of Luo Han Guo flavonoids tipping paper for cigarettes |
| CN113398157A (en) * | 2021-07-14 | 2021-09-17 | 湖南华诚生物资源股份有限公司 | Method for continuously extracting and separating multiple natural active ingredients from momordica grosvenori flower |
| CN115581266A (en) * | 2022-09-02 | 2023-01-10 | 湖南华诚生物资源股份有限公司 | Clean production method of momordica grosvenori dry powder and momordica grosvenori condensed juice |
| CN115581266B (en) * | 2022-09-02 | 2024-03-12 | 湖南华诚生物资源股份有限公司 | Clean production method of momordica grosvenori dry powder and momordica grosvenori concentrated juice |
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