CN1359004A - Urinary protein chip for quick testing kidney damage - Google Patents
Urinary protein chip for quick testing kidney damage Download PDFInfo
- Publication number
- CN1359004A CN1359004A CN 01126895 CN01126895A CN1359004A CN 1359004 A CN1359004 A CN 1359004A CN 01126895 CN01126895 CN 01126895 CN 01126895 A CN01126895 A CN 01126895A CN 1359004 A CN1359004 A CN 1359004A
- Authority
- CN
- China
- Prior art keywords
- protein
- carrier box
- test strips
- urine
- urinary
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 59
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 59
- 230000002485 urinary effect Effects 0.000 title claims abstract description 24
- 210000003734 kidney Anatomy 0.000 title claims abstract description 21
- 230000006378 damage Effects 0.000 title claims abstract description 19
- 210000002700 urine Anatomy 0.000 claims abstract description 34
- 102000009027 Albumins Human genes 0.000 claims abstract description 14
- 108010088751 Albumins Proteins 0.000 claims abstract description 14
- 102000002070 Transferrins Human genes 0.000 claims description 12
- 108010015865 Transferrins Proteins 0.000 claims description 12
- 238000002965 ELISA Methods 0.000 claims description 10
- 238000002347 injection Methods 0.000 claims description 7
- 239000007924 injection Substances 0.000 claims description 7
- 238000002331 protein detection Methods 0.000 claims 1
- 102000004338 Transferrin Human genes 0.000 abstract 2
- 108090000901 Transferrin Proteins 0.000 abstract 2
- 208000027418 Wounds and injury Diseases 0.000 abstract 2
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 abstract 2
- 208000014674 injury Diseases 0.000 abstract 2
- 239000012581 transferrin Substances 0.000 abstract 2
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 abstract 1
- 102000004366 Glucosidases Human genes 0.000 abstract 1
- 108010056771 Glucosidases Proteins 0.000 abstract 1
- 229960003471 retinol Drugs 0.000 abstract 1
- 235000020944 retinol Nutrition 0.000 abstract 1
- 239000011607 retinol Substances 0.000 abstract 1
- 235000018102 proteins Nutrition 0.000 description 43
- 238000001514 detection method Methods 0.000 description 10
- 238000000034 method Methods 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 238000003127 radioimmunoassay Methods 0.000 description 4
- 102000003966 Alpha-1-microglobulin Human genes 0.000 description 3
- 101800001761 Alpha-1-microglobulin Proteins 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 238000007689 inspection Methods 0.000 description 3
- 238000003908 quality control method Methods 0.000 description 2
- 210000005239 tubule Anatomy 0.000 description 2
- 206010020772 Hypertension Diseases 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 206010012601 diabetes mellitus Diseases 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000004907 flux Effects 0.000 description 1
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 1
- 239000010931 gold Substances 0.000 description 1
- 229910052737 gold Inorganic materials 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
Images
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention discloses a urinary protein chip for quickly-detecting injury of kidney, and is mainly characterized by that said urinary protein chip is formed from carrier box face plate and carrier box back plate for storing uninary protein test strips, in the interior of carrier box back plate more than four (including four) carrier channels are set, and in the interior of carrier channel at least four protein test strips for detecting urinary albumin (ALB), transferrin (TF), retinol conjugated protein (RBP) and urine N-acetyl-beta-D glucosidase (NAG) are placed. Besides, the protein test strip made of other protein specific antibody also can be added. Said invention can be used for early diagnosing injury of kidney and making primary judgement of injured region.
Description
The present invention relates to a kind of pick-up unit, particularly relate to a kind of urinary protein chip of fast detecting kidney damage.
Numerous disease all can cause kidney damage, as disease of diabetes, hypertension, rheumatism systemic disease and kidney itself etc.Conventional inspection can only find that urine albumen amount surpasses 150mg/ days patient, can not distinguish the composition of urine protein.So, clinical more Useful Informations that provide are provided.Early stage kidney damage patient's urine albumen amount is often less than 150mg/ days, and conventional detection method Chang Buneng finds the variation of urine protein, forms the change that diagnostic significance has taken place to have but urinate trace protein this moment.These protein comprise: urinary albumin (ALB), α 1 microglobulin (α 1-MG), β2Wei Qiudanbai (β 2-MG), transferrins (TF), RBP ELISA (RBP), urine N-acetyl-β-D glucuroide (NAG), IgG or the like.The clinical detection trace protein diagnoses the method for early stage renal damage that radio immunoassay (RIA), enzyme linked immunosorbent assay method (ELISA) etc. are arranged at present, these inspections are comparatively loaded down with trivial details, it is long to last, expense is higher and can only detect a kind of albumen at every turn, bring inconvenience to patient and clinician's diagnosis and observation of curative effect.Though with the single protein test paper of preparation such as golden immunochromatographic method easily and fast, also can only measure a kind of urine protein, the amount of diagnostic information that provides is limited and not comprehensive.
The objective of the invention is to provide a kind of urinary protein chip of fast detecting kidney damage, it can not only disposable while fast detecting, distinguish the variation that protein component in the urine is formed and can be found to multiple proteins in the urine, method of operating is simple, convenient, the damage location that helps the infringement of early detection kidney and tentatively judge kidney.
For achieving the above object, the technical solution used in the present invention is: a kind of urinary protein chip of fast detecting kidney damage, it is by the carrier box panel, carrier box backboard and protein test strips are formed, be provided with the carrier channels that to place four above test strips in the described carrier box backboard, described test strips is by the suction zones of upper end, the observation district of middle-end and the sample introduction district of lower end constitute, described panel bottom is an injection port, the middle part is the control line-transect between observation panel and injection port and the observation panel, four carrier channels that are the arrangement of fan property are arranged in the described carrier box backboard, place respectively in the carrier channels and detect urinary albumin (ALB), transferrins (TF), RBP ELISA (RBP), the urine protein test strips of urine N-acetyl-β-D glucuroide (NAG), utilize the integrated of chip technology, high flux, parallel principle, with these protein test paper with parallel or fan-shaped mode combination in any, integrate, form urinary protein chip, it can the disposable composition that detects these four kinds of protein in the urine simultaneously.Wherein urinary albumin (ALB) and transferrins (TF) mainly reflect the function of glomerulus, and RBP ELISA (RBP) and urine N-acetyl-β-D glucuroide (NAG) mainly reflect the function of renal tubule.The urinary protein chip of fast detecting kidney damage can be on the basis of above four kinds of test strips, add the test strips that other urine protein specific antibodies are made, as α 1 microglobulin (α 1-MG), β2Wei Qiudanbai (β 2-MG) and IgG, form the integrated urinary protein chip of urine protein test strips more than four kinds, detect the composition of the multiple proteins more than four kinds simultaneously, the urine protein composition that increase to detect other also has certain help to the detection of early stage kidney damage.The joint-detection of multiple urine trace protein not only increases the susceptibility and the specificity of inspection, but also can reduce the consumption of test sample significantly.
This urinary protein chip can be revised slightly as required and can be made into multiple shape, as circle, rectangle or square or the like, also belongs to the protection domain of this patent.
The present invention has improved the shortcoming that the operation of radio immunoassay (RIA), enzyme linked immunosorbent assay method (ELISA) is loaded down with trivial details, time-consuming, cost an arm and a leg and once can only detect a kind of urine protein.The present invention once can finish the detection of urine protein more than four kinds, but the not only infringement of early detection kidney but also can tentatively judge the position of kidney damage, simple to operate, easy to use, without any need for other equipment, with the naked eye can be immediately, observations accurately, thereby have very high clinical use value.
Below in conjunction with accompanying drawing the embodiment of the invention is described in further detail.
Fig. 1 shows the urinary protein chip carrier box backboard synoptic diagram of fast detecting kidney damage;
Fig. 2 shows the urinary protein chip carrier box synoptic diagram of fast detecting kidney damage;
Fig. 3 shows urine protein test paper synoptic diagram.
With reference to Fig. 1, Fig. 2 and Fig. 3, the urinary protein chip of fast detecting kidney damage of the present invention, it is made up of carrier box (1) and protein test strips (8), described carrier box is made up of carrier box panel (2) and carrier box backboard (3), be provided with in the carrier box can deposit four protein test strips be fan property arrangement carrier channels (4), described test strips has the suction zones (11) of upper end, the observation district (10) of middle-end and the sample introduction district (9) of lower end constitute, described carrier box panel bottom is injection port (5), the middle part is the control line-transect (7) between observation panel (6) and injection port (5) and the observation panel (6), places respectively in the carrier channels and can detect albumin (ALB), transferrins (TF), RBP ELISA (RBP), the protein test paper (8) of urine N-acetyl-β-D glucuroide (NAG).Used urine protein test strips also can be on the basis of above four kinds of test paper, add the test strips that other urine protein specific antibodies are made, as α 1 microglobulin (α 1-MG), β2Wei Qiudanbai (β 2-MG) and IgG, form the fan-shaped integrated urinary protein chip of urine protein test strips more than four kinds, detect the composition of the multiple urine protein more than four kinds simultaneously.
During use, when when injection port (5) adds the urine specimen that will detect, each protein in the urine can combine with the golden labeling antibody in the sample introduction district (9) on the urine protein test strips of this protein of urinary protein chip relevant detection, distinguish (10) along the membrane chromatographic of each test paper to observing respectively, and with detection line on the bag quilt specific antibody combine, in observation panel (6), form macroscopic red stripes, excessive compound or unconjugated gold mark thing can continue chromatography, the macroscopic red lines of formation combine with the antibody of control line (quality control line), article two, red lines then show the positive, if have only the quality control line to form red lines then show feminine gender, if detection line and quality line all not colour developing show invalid.The function that detects the positive explanation of the test strips glomerulus of urinary albumin (ALB) and transferrins (TF) has infringement, detects the test strips positive of RBP ELISA (RBP) and urine N-acetyl-β-D glucuroide (NAG) and then points out the function of renal tubule that infringement is arranged.
Should be understood that these embodiment only to be used to the present invention is described and be not used in and limit the scope of the invention.The experimental technique of unreceipted actual conditions in the following example, usually according to the condition described in normal condition such as the laboratory manual, or the condition of advising according to manufacturer.
Claims (3)
1. the urinary protein chip of a fast detecting kidney damage, it is made up of carrier box and protein test strips (8), described carrier box is made up of carrier box panel (2) and carrier box backboard (3), the middle part of carrier box panel is provided with observation panel (6), the bottom is provided with injection port (5), be provided with control line-transect (7) between observation panel and the injection port, the upper end of protein test strips is suction zones (11), middle-end is for observing district (10), the lower end is sample introduction district (9), it is characterized in that described carrier box backboard has the carrier channels (4) that (comprises four) more than four and deposit the urine protein test strips, lays at least in the described carrier channels and can detect urinary albumin (ALB), transferrins (TF), RBP ELISA (RBP) and urine N-acetyl-β-four kinds of protein test strips of D glucuroide (NAG).
2. according to the urinary protein chip of the described fast detecting kidney damage of claim 1, it is characterized in that in the carrier channels in the described carrier box backboard can also addition by the urine protein test strips made from other urine protein specific antibodies.
3. urine protein detection chip according to claim 1 is characterized in that the load sample groove (4) in the described carrier box backboard (3) is single groove of more than four of fan-shaped array (containing four).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01126895 CN1359004A (en) | 2001-09-28 | 2001-09-28 | Urinary protein chip for quick testing kidney damage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 01126895 CN1359004A (en) | 2001-09-28 | 2001-09-28 | Urinary protein chip for quick testing kidney damage |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1359004A true CN1359004A (en) | 2002-07-17 |
Family
ID=4666898
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 01126895 Pending CN1359004A (en) | 2001-09-28 | 2001-09-28 | Urinary protein chip for quick testing kidney damage |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1359004A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103424552A (en) * | 2012-05-21 | 2013-12-04 | 天津华生源科技有限公司 | Test card for detecting human glycated albumin |
| CN105300971A (en) * | 2015-09-21 | 2016-02-03 | 济南大学 | Preparation method of urine protein detection test paper |
| CN106290824A (en) * | 2016-10-19 | 2017-01-04 | 中国科学院电子学研究所 | Two-parameter fan-shaped immunity magnetosphere analysis strip |
| CN106443012A (en) * | 2016-09-12 | 2017-02-22 | 三诺生物传感股份有限公司 | Test strip, preparation method thereof and application of test strip to combined detection of microalbuminuria and beta2 microglobulin |
| CN109642902A (en) * | 2016-06-27 | 2019-04-16 | Tgr生物科学私人有限公司 | Lateral flow analyte detection |
-
2001
- 2001-09-28 CN CN 01126895 patent/CN1359004A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103424552A (en) * | 2012-05-21 | 2013-12-04 | 天津华生源科技有限公司 | Test card for detecting human glycated albumin |
| CN105300971A (en) * | 2015-09-21 | 2016-02-03 | 济南大学 | Preparation method of urine protein detection test paper |
| CN109642902A (en) * | 2016-06-27 | 2019-04-16 | Tgr生物科学私人有限公司 | Lateral flow analyte detection |
| CN106443012A (en) * | 2016-09-12 | 2017-02-22 | 三诺生物传感股份有限公司 | Test strip, preparation method thereof and application of test strip to combined detection of microalbuminuria and beta2 microglobulin |
| CN106443012B (en) * | 2016-09-12 | 2018-11-06 | 三诺生物传感股份有限公司 | A kind of test strips and preparation method thereof and the application in microdose urine protein and β2-microglobulin joint-detection |
| CN106290824A (en) * | 2016-10-19 | 2017-01-04 | 中国科学院电子学研究所 | Two-parameter fan-shaped immunity magnetosphere analysis strip |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Choi et al. | A rapid, simple measurement of human albumin in whole blood using a fluorescence immunoassay (I) | |
| Hedström et al. | Urine trypsinogen-2 as marker of acute pancreatitis | |
| O'Mahony et al. | Dissociation between systemic and mucosal humoral immune responses in coeliac disease. | |
| ES2250627T3 (en) | URINE TEST FOR OVARIC RESERVE. | |
| CN102707067A (en) | Test strip for semi-quantitative detection of microalbuminuria | |
| CN102207507A (en) | Semi-quantitative detecting test paper of cardiac troponin and preparation method thereof | |
| Kotoula et al. | Procalcitonin for the early prediction of renal parenchymal involvement in children with UTI: preliminary results | |
| US20240255532A1 (en) | Biomarkers for the Detection of Thrombosis or Coagulation-Related Disorders and Their Applications | |
| US20120115174A1 (en) | Urinary trypsin inhibitors as diagnostic aid for interstitial cystitis | |
| CN101023356A (en) | Method for diagnosing liver fibrosis | |
| CN109669035A (en) | The detection method of detection kit and blood sample | |
| CN1359004A (en) | Urinary protein chip for quick testing kidney damage | |
| ES2402916T3 (en) | Test method with membrane and kit | |
| CN206638678U (en) | Multi objective time-resolved fluoroimmunoassay for postoperative patient monitoring chromatographs kit | |
| KR102339013B1 (en) | In-vitro diagnostic kit capable of quantitating mmp-8 in amniotic fluid specimen | |
| CN201096787Y (en) | C-reaction albumen color grain diagnosis testing paper | |
| CN1346892A (en) | Biochip for joint detection of gastrosis | |
| CN1348105A (en) | Combined hepatosis testing biochip | |
| JP2004028875A (en) | Simple membrane assay and kit | |
| CN207472899U (en) | A kind of kit for quantitatively detecting NGAL in urine or blood | |
| CN2395277Y (en) | Combination Antigen Echinococcosis Rapid Diagnosis Reaction Panel | |
| CN1896740B (en) | Colloidal gold test strip and preparation method for semi-quantitative rapid detection of oxidized low-density lipoprotein | |
| JP3522764B2 (en) | Assay of free and conjugated trypsinogen-2 | |
| CN102971630A (en) | Marker for detection and/or discrimination of non-alcoholic steatohepatitis, method for detection and/or discrimination of non-alcoholic steatohepatitis, and kit for use in the method | |
| CN2528017Y (en) | Protein detecting chip |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |