CN120859896A - Chinese herbal medicine trichromatic strip toothpaste and preparation method thereof - Google Patents
Chinese herbal medicine trichromatic strip toothpaste and preparation method thereofInfo
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- CN120859896A CN120859896A CN202511035888.7A CN202511035888A CN120859896A CN 120859896 A CN120859896 A CN 120859896A CN 202511035888 A CN202511035888 A CN 202511035888A CN 120859896 A CN120859896 A CN 120859896A
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Abstract
The application belongs to the technical field of daily chemicals, and particularly relates to a Chinese herbal medicine trichromatic strip toothpaste and a preparation method thereof. The Chinese herbal medicine three-color strip toothpaste comprises a light yellow paste, a green paste and a blue paste, wherein the light yellow paste comprises 0.1-2% of chitosan quaternary ammonium salt, 0.1-1% of cortex rotundae officinalis extract by mass, the green paste comprises 0.002-0.15% of sodium copper chlorophyllin and 0.1-1% of cortex rotundae officinalis extract by mass, and the blue paste comprises 0.03-0.15% of guaiacum sodium sulfonate and 0.1-1% of cortex rotundae officinalis extract by mass. According to the Chinese herbal medicine trichromatic color bar toothpaste and the preparation method thereof, the trichromatic color bar paste toothpaste is obtained under the condition that exogenous pigments are not added, and the toothpaste can realize the composite effects of freshening breath, inhibiting dental plaque and oral pathogenic bacteria, resisting inflammation and detumescence, resisting sensitization, relieving gingivitis, promoting healing and repairing of recurrent oral ulcer, promoting remineralization of dental enamel and pore repair, has strong efficacy cooperativity, stability of paste and higher use safety.
Description
Technical Field
The application belongs to the technical field of daily chemicals, and particularly relates to a Chinese herbal medicine trichromatic strip toothpaste and a preparation method thereof.
Background
With the continuous progress of toothpaste production technology and the continuous improvement of people's living standard, more and more consumers are focusing on the oral health and the attractive appearance of teeth. The current global oral care market shows remarkable functionalization and naturalization trends, and the demands of consumers on traditional Chinese medicine toothpastes with multiple effects are continuously increased, the worry of chemical synthesis components and the preference of herbal care are improved, so that the traditional Chinese medicine toothpastes are promoted to be upgraded from the traditional partial effects of anti-inflammation, bacteriostasis, hemostasis and breath refreshing to the traditional anti-inflammation, bacteriostasis, hemostasis and breath refreshing composite effects.
At present, the mainstream toothpaste products mostly depend on a single component to realize specific functions (such as fluoride mothproof and plant essential oil bacteriostasis), have single action mechanism, have the problem of lacking systematic solution to complex oral pathology such as gingivitis and plaque accumulation (for example, salicylic acid derivatives only can relieve gingival redness and swelling but can not inhibit plaque biofilm formation to cause inflammation repetition), and partial alleged multi-effect products easily cause antagonism among components through simple mixing of traditional Chinese medicine extracts, have insufficient efficacy cooperativity, and in addition, the toothpaste is added with exogenous pigment to improve visual attraction, instruct efficacy and enhance sensory experience, but have the defects of safety risk, stability problem and possibility of affecting the appearance of tooth surface due to long-term use.
In view of the above, it is desirable to provide a Chinese herbal toothpaste without pigment, which systematically solves the above problems and provides an innovative solution for oral health management.
Disclosure of Invention
The application aims to provide a Chinese herbal medicine trichromatic strip toothpaste and a preparation method thereof, which aim to solve at least one of the technical problems.
In order to achieve the aim, the application provides the three-color strip toothpaste of the Chinese herbal medicine, which comprises a light yellow paste, a green paste and a blue paste, wherein the light yellow paste comprises 0.1-2% of chitosan quaternary ammonium salt, 0.1-1% of cortex rotundae officinalis extract by mass, the green paste comprises 0.002-0.15% of sodium copper chlorophyllin and 0.1-1% of cortex rotundae officinalis extract by mass, and the blue paste comprises 0.03-0.15% of guayule sodium sulfonate and 0.1-1% of cortex rotundae officinalis extract by mass.
In some embodiments of the application, the Chinese herbal medicine trichromatic strip toothpaste comprises a yellowish paste, a green paste and a blue paste=8-6:1-2.5:1-2.5 by mass.
In some embodiments of the application, the Chinese herbal medicine trichromatic strip toothpaste comprises a light yellow paste, a green paste, a blue paste=7:1.5:1.5 or a light yellow paste, a green paste, a blue paste=6:2:2. In some embodiments of the present application, the yellowish paste, green paste, and blue paste each include 0.001% -0.05% rosemary extract, 0.005% -0.5% dipotassium glycyrrhizinate, and 0.005% -1.0% arginine by mass.
In some embodiments of the application, the light yellow paste, the green paste and the blue paste further comprise, by mass, 45% -65% of a humectant, 16% -22% of a friction agent, 0.4% -1.4% of a thickener, 1.0% -3.0% of a surfactant, 0.5% -1.3% of an essence, 0.1% -0.22% of a sweetener, 0% -0.5% of a preservative, 0.1% -0.5% of a stabilizer, and the balance water.
In some embodiments of the application, the humectant is one or more of sorbitol, polyethylene glycol (PEG 400), glycerin, propylene glycol.
In some embodiments of the application, the abrasive is one or more of hydrated silica, calcium carbonate, calcium phosphate, aluminum hydroxide.
In some embodiments of the application, the thickener is one or more of sodium carboxymethylcellulose (CMC), hydroxyethylcellulose (HEC), polyquaternium-22, carrageenan, xanthan gum, guar gum, carbomer, xanthan gum.
In some embodiments of the application, the surfactant is one or more of sodium dodecyl sulfate (K12), sodium lauryl sulfate, lauryl glucoside, sodium lauroyl sarcosinate, betaine, sodium lauroyl glutamate, sodium cocoyl methyl taurate.
In some embodiments of the application, the sweetener is one or more of sodium saccharin, sucralose, xylitol, erythritol, steviol glycosides.
In some embodiments of the application, the preservative is one or more of sodium benzoate, parabens and salts thereof, polyols.
In some embodiments of the application, the stabilizer is one or more of pyrophosphate and phosphate.
The application provides a preparation method of Chinese herbal medicine trichromatic strip toothpaste, which comprises the following steps of:
1. weighing deionized water (a residual proper amount of flushing container), sequentially adding 0.1-0.22% of sweetener, 0-0.5% of preservative, 0.1-0.5% of stabilizer, 0.005-0.5% of dipotassium glycyrrhizinate and 0.005-1.0% of arginine according to mass fraction, and uniformly stirring and dissolving for later use;
2. weighing 45-65% of humectant for standby;
3. weighing 16-22% of friction agent, 0.4-1.4% of thickening agent, 0.1-2% of chitosan quaternary ammonium salt and 0.1-1% of cortex rotundae officinalis extract by mass fraction, and uniformly premixing in a stainless steel pot;
4. dissolving 0.01-0.5% of cooling agent and 0.005-0.1% of rosemary extract in 0.5-1.3% of essence for later use;
5. weighing 1.0-3.0% of surfactant for standby;
6. adding the sample in the step 2 into an ointment making pot, then adding the sample in the step 1, flushing a container with the rest deionized water, and stirring for 3min by a vacuum scraper;
7. adding the sample in the step 3, starting a scraper, stirring until no obvious dry powder exists, starting vacuum, and starting timing for 10min when the vacuum reaches more than-0.092 MPa;
8. The samples added in 4 and 5 are stirred for 30min by a vacuum scraper (the vacuum is not higher than-0.094 MPa);
9. turning off the scraping plate, stirring, and vacuum degassing for more than 10min until no obvious bubbles exist, thus obtaining the yellowish main paste.
In some embodiments of the application, the light yellow main paste preparation step 1 is added with saccharin sodium with the mass fraction of 0.1%, sodium benzoate with the mass fraction of 0.2%, sodium pyrophosphate with the mass fraction of 0.3%, dipotassium glycyrrhizinate with the mass fraction of 0.2% and arginine with the mass fraction of 0.8%.
In some embodiments of the application, the light yellow main paste preparation step 2 comprises weighing 50% of sorbitol, 3% of PEG400 and 8% of glycerin by mass.
In some embodiments of the present application, 20% hydrated silica, 0.3% xanthan gum, 0.6% polyquaternium-22, 0.3% guar gum, 0.5% chitosan quaternary ammonium salt, and 0.6% holly bark extract are weighed in the light yellow main paste preparation step 3.
In some embodiments of the present application, the light yellow main paste preparation step 4 comprises dissolving 0.1% of cooling agent and 0.5% of rosemary extract in 1% of essence.
In some embodiments of the application, the light yellow main paste is prepared by taking 2.0% of K12, 0.5% of sodium lauroyl sarcosine and 0.5% of betaine according to the mass fraction in the preparation step 5 for later use.
In some embodiments of the application, the method of preparation comprises the steps of:
1. Weighing 0.5-1% of deionized water and dissolving 0.002-0.15% of sodium copper chlorophyllin;
2. weighing deionized water (a residual proper amount of flushing container), adding 0.1-0.22% of sweetener, 0-0.5% of preservative, 0.1-0.5% of stabilizer, 0.005-0.5% of dipotassium glycyrrhizinate and 0.005-1% of arginine by mass fraction, and uniformly stirring and dissolving for later use;
3. weighing 45-65% of humectant for standby;
4. weighing 16-22% of friction agent, 0.4-1.4% of thickening agent and 0.1-1% of cortex rotundae officinalis extract by mass fraction, and uniformly premixing in a stainless steel pot;
5. Dissolving 0.01-0.5% of cooling agent and 0.001-0.1% of rosemary extract in 0.5-1.3% of essence for standby;
6. weighing 1.0-3.0% of surfactant for standby;
7. Adding the sample in the step 3 into an ointment making pot, adding the sample in the step 2 and the step 1, flushing a container with the rest deionized water, and stirring for 3min by a vacuum scraper;
8. adding the sample in the step 4, starting a scraper, stirring until no obvious dry powder exists, starting vacuum, and starting timing for 10min when the vacuum reaches more than-0.092 MPa;
9. adding the samples in the step 5 and the step 6, starting a vacuum scraper and stirring for 30min (the vacuum is not higher than-0.094 MPa);
10. turning off the scraping plate, stirring, and vacuum degassing for more than 10min until no obvious bubbles exist, thus obtaining the green auxiliary paste.
In some embodiments of the application, the green auxiliary paste preparation step 1 comprises weighing 0.5% of deionized and dissolved 0.05% sodium copper chlorophyllin by mass.
In some embodiments of the application, the green auxiliary paste preparation step 2 is added with saccharin sodium 0.1%, sodium benzoate 0.3%, sodium pyrophosphate 0.3%, dipotassium glycyrrhizinate 0.2% and arginine 0.8% by mass.
In some embodiments of the application, the green auxiliary paste preparation step 3 comprises weighing 50% of sorbitol, 3% of PEG400 and 8% of glycerin by mass.
In some embodiments of the present application, the green auxiliary paste preparation step 4 comprises weighing 20% hydrated silica, 0.3% xanthan gum, 0.6% polyquaternium-22, 0.3% guar gum, and 0.4% cortex Ilicis Rotundae extract.
In some embodiments of the present application, the green auxiliary paste preparation step 5 comprises dissolving 0.1% cooling agent and 0.05% rosemary extract in 1% essence.
In some embodiments of the application, the green auxiliary paste preparation step 6 comprises taking 2.0% of K12, 0.5% of sodium lauroyl sarcosine and 0.5% of betaine for standby.
In some embodiments of the application, the preparation method comprises the steps of:
1. Weighing 0.5% deionized water to dissolve 0.1% guaiac sodium azulene sulfonate;
2. Weighing deionized water (a residual proper amount of flushing container), sequentially adding 0.1-0.22% of sweetener, 0-0.5% of preservative, 0.1-0.5% of stabilizer, 0.005-0.5% of dipotassium glycyrrhizinate and 0.005-1% of arginine according to mass fraction, and uniformly stirring and dissolving for later use;
3. Weighing 45% -65% of humectant for standby;
4. weighing 16-22% of friction agent, 0.4-1.4% of thickening agent and 0.1-1% of cortex rotundae officinalis extract by mass fraction, and uniformly premixing in a stainless steel pot;
5. dissolving 0.01-0.5% of cooling agent and 0.005-0.1% of rosemary extract in 0.5-1.3% of essence for later use;
6. weighing 1.0-3.0% of surfactant for standby;
7. Adding the sample in the step 3 into an ointment making pot, adding the sample in the step 2 and the step 1, flushing a container with the rest deionized water, and stirring for 3min by a vacuum scraper;
8. adding the sample in the step 4, starting a scraper, stirring until no obvious dry powder exists, starting vacuum, and starting timing for 10min when the vacuum reaches more than-0.092 MPa;
9. adding the samples in the step 5 and the step 6, starting a vacuum scraper and stirring for 30min (the vacuum is not higher than-0.094 MPa);
10. turning off the scraping plate, stirring, and vacuum degassing for more than 10min until no obvious bubbles exist, thus obtaining the blue auxiliary paste.
In some embodiments of the present application, the mass fraction of the weighed deionized water in the blue auxiliary paste preparation step 1 is 0.5% to dissolve 0.1% of sodium guaiacol sulfonate.
In some embodiments of the application, the blue auxiliary paste preparation step 2 is added with saccharin sodium 0.1%, sodium benzoate 0.3%, sodium pyrophosphate 0.3%, dipotassium glycyrrhizinate 0.2% and arginine 0.8% by mass.
In some embodiments of the application, the mass fraction of the blue auxiliary paste in the preparation step 3 is 50% of sorbitol, 3% of PEG400 and 8% of glycerin.
In some embodiments of the present application, 20% hydrated silica, 0.3% xanthan gum, 0.6% polyquaternium-22, 0.3% guar gum, 0.4% holly bark extract are weighed in the blue auxiliary paste preparation step 4.
In some embodiments of the present application, the green auxiliary paste preparation step 5 comprises dissolving 0.1% cooling agent and 0.05% rosemary extract in 1% essence.
In some embodiments of the present application, the blue auxiliary paste preparation step 6 comprises 0.5% of K12, 0.5% of sodium lauroyl sarcosine and 0.5% of betaine by mass.
In some embodiments of the application, the preparation method further comprises a filling step of filling the three-color paste into a toothpaste tube together according to the mass ratio of light yellow main paste to green auxiliary paste to blue auxiliary paste=8-5:1-2.5:1-2.5 to obtain the Chinese herbal medicine three-color strip toothpaste.
In some embodiments of the application, the mass ratio of the yellowish main paste to the green subsidiary paste to the blue subsidiary paste=7:1.5:1.5 or the mass ratio of the yellowish main paste to the green subsidiary paste to the blue subsidiary paste=6:2:2 in the filling step.
According to the Chinese herbal medicine trichromatic color bar toothpaste and the preparation method thereof, the trichromatic color bar paste toothpaste is obtained under the condition that exogenous pigments are not added, and the toothpaste can realize the composite effects of freshening breath, inhibiting dental plaque and oral pathogenic bacteria, resisting inflammation and detumescence, resisting sensitization, relieving gingivitis, promoting healing and repairing of recurrent oral ulcer, promoting remineralization of dental enamel and pore repair, has strong efficacy cooperativity, stability of paste and higher use safety.
Detailed Description
In the present application, the numerical range indicated by "-" means a range in which numerical values described before and after "-" are included as a minimum value and a maximum value, respectively.
In the numerical ranges described in stages in the present application, the upper limit or the lower limit described in a certain numerical range may be replaced with the upper limit or the lower limit of another numerical range described in stages. In the numerical ranges described in the present application, the upper limit value or the lower limit value described in a certain numerical range may be replaced with the value shown in the embodiment.
The terms "comprising," "including," or "containing," when used in this specification, specify the presence of stated elements, but do not preclude the presence or addition of one or more other elements, unless the context clearly indicates otherwise.
In order that the above objects, features and advantages of the present application will become more readily apparent, a more particular description of the application will be rendered by reference to specific examples and test examples. In the following description, numerous specific details are set forth in order to provide a thorough understanding of the present application. The present application may be embodied in many other forms than described herein and similarly modified by those skilled in the art without departing from the spirit of the application, whereby the application is not limited to the specific embodiments disclosed below.
The cortex rotundae officinalis is used as a traditional folk Chinese herbal medicine in southern areas of China, the medicinal record of the cortex rotten holly bark is traced to the record of Ling nan Ming Yao Ji in 1932 and formally received in Chinese pharmacopoeia (one part) in 1977, and the cortex rotten holly bark has definite effects of purging pathogenic fire, removing toxin, cooling blood and relieving pain, and is widely applied to the treatment of injuries from falls, scalds and burns and other trauma. The extraction process takes dried bark of Ilex rotunda Thunb (Ilex rotunda Thunb) of iledaceae as a raw material, and finally prepares the characteristic light yellow powder through ethanol extraction (ethanol extraction), separation and purification and drying treatment, wherein the extract is rich in active ingredients such as triterpenoid saponins, flavonoids and the like, and is an important raw material of functional products (such as anti-inflammatory toothpaste and external analgesic ointment) in the fields of daily chemicals and medicines.
The application discloses a three-color strip toothpaste of Chinese herbal medicine and a preparation method thereof, and aims to solve the technical problems that the function and action mechanism of the toothpaste are single, systematic solutions are lacking in complex oral pathology, antagonism among components is easily caused by partially claiming a multi-effect product by simply mixing Chinese herbal medicine extracts, the efficacy and the cooperativity are insufficient, and in addition, the safety risk and the stability problems and the technical problems that the tooth surface coloring influences the appearance possibly caused by long-term use are caused by adding exogenous pigments into the toothpaste.
The application provides a preparation method of Chinese herbal medicine trichromatic strip toothpaste, which comprises the following steps of:
1. Weighing deionized water (a residual proper amount of flushing container), sequentially adding 0.1-0.22% of sweetener, 0-0.5% of preservative, 0.1-0.5% of stabilizer, 0.005-0.5% of dipotassium glycyrrhizinate and 0.005-1% of arginine according to mass fraction, and uniformly stirring and dissolving for later use;
2. weighing 45-65% of humectant for standby;
3. weighing 16-22% of friction agent, 0.4-1.4% of thickening agent, 0.1-2% of chitosan quaternary ammonium salt and 0.1-1% of cortex rotundae officinalis extract by mass fraction, and uniformly premixing in a stainless steel pot;
4. dissolving 0.01-0.5% of cooling agent and 0.005-0.1% of rosemary extract in 0.5-1.3% of essence for later use;
5. weighing 1.0-3.0% of surfactant for standby;
6. adding the sample in the step 2 into an ointment making pot, then adding the sample in the step 1, flushing a container with the rest deionized water, and stirring for 3min by a vacuum scraper;
7. adding the sample in the step 3, starting a scraper, stirring until no obvious dry powder exists, starting vacuum, and starting timing for 10min when the vacuum reaches more than-0.092 MPa;
8. The samples added in 4 and 5 are stirred for 30min by a vacuum scraper (the vacuum is not higher than-0.094 MPa);
9. turning off the scraping plate, stirring, and vacuum degassing for more than 10min until no obvious bubbles exist, thus obtaining the yellowish main paste.
In some embodiments of the application, the method of preparation comprises the steps of:
1. Weighing 0.5-1% of deionized water and dissolving 0.002-0.15% of sodium copper chlorophyllin;
2. weighing deionized water (a residual proper amount of flushing container), adding 0.1-0.22% of sweetener, 0-0.5% of preservative, 0.1-0.5% of stabilizer, 0.005-0.5% of dipotassium glycyrrhizinate and 0.005-1.0% of arginine by mass percent, and uniformly stirring and dissolving for later use;
3. weighing 45-65% of humectant for standby;
4. weighing 16-22% of friction agent, 0.4-1.4% of thickening agent and 0.1-1% of cortex rotundae officinalis extract by mass fraction, and uniformly premixing in a stainless steel pot;
5. dissolving 0.01-0.5% of cooling agent and 0.005-0.1% of rosemary extract in 0.5-1.3% of essence for later use;
6. weighing 1.0-3.0% of surfactant for standby;
7. Adding the sample in the step 3 into an ointment making pot, adding the sample in the step 2 and the step 1, flushing a container with the rest deionized water, and stirring for 3min by a vacuum scraper;
8. adding the sample in the step 4, starting a scraper, stirring until no obvious dry powder exists, starting vacuum, and starting timing for 10min when the vacuum reaches more than-0.092 MPa;
9. adding the samples in the step 5 and the step 6, starting a vacuum scraper and stirring for 30min (the vacuum is not higher than-0.094 MPa);
10. turning off the scraping plate, stirring, and vacuum degassing for more than 10min until no obvious bubbles exist, thus obtaining the green auxiliary paste.
In some embodiments of the application, the green auxiliary paste preparation step 1 comprises weighing 0.5% of deionized and dissolved 0.05% sodium copper chlorophyllin by mass.
In some embodiments of the application, the preparation method comprises the steps of:
1. Weighing 0.5% deionized water to dissolve 0.1% guaiac sodium azulene sulfonate;
2. Weighing deionized water (a residual proper amount of flushing container), sequentially adding 0.1-0.22% of sweetener, 0-0.5% of preservative, 0.1-0.5% of stabilizer, 0.005-0.5% of dipotassium glycyrrhizinate and 0.005-1.0% of arginine according to mass fraction, and uniformly stirring and dissolving for later use;
3. Weighing 45% -65% of humectant for standby;
4. weighing 16-22% of friction agent, 0.4-1.4% of thickening agent and 0.1-1% of cortex rotundae officinalis extract by mass fraction, and uniformly premixing in a stainless steel pot;
5. dissolving 0.01-0.5% of cooling agent and 0.005-0.1% of rosemary extract in 0.5-1.3% of essence for later use;
6. weighing 1.0-3.0% of surfactant for standby;
7. Adding the sample in the step 3 into an ointment making pot, adding the sample in the step 2 and the step 1, flushing a container with the rest deionized water, and stirring for 3min by a vacuum scraper;
8. adding the sample in the step 4, starting a scraper, stirring until no obvious dry powder exists, starting vacuum, and starting timing for 10min when the vacuum reaches more than-0.092 MPa;
9. adding the samples in the step 5 and the step 6, starting a vacuum scraper and stirring for 30min (the vacuum is not higher than-0.094 MPa);
10. turning off the scraping plate, stirring, and vacuum degassing for more than 10min until no obvious bubbles exist, thus obtaining the blue auxiliary paste.
In some embodiments of the application, the humectant is one or more of sorbitol, polyethylene glycol (PEG 400), glycerin, propylene glycol.
In some embodiments of the application, the abrasive is one or more of hydrated silica, calcium carbonate, calcium phosphate, aluminum hydroxide.
In some embodiments of the application, the thickener is one or more of sodium carboxymethyl cellulose (CMC), hydroxyethyl cellulose (HEC), carrageenan, xanthan gum, polyquaternium-22, guar gum, carbomer, xanthan gum.
In some embodiments of the application, the surfactant is one or more of sodium dodecyl sulfate (K12), sodium lauryl sulfate, lauryl glucoside, sodium lauroyl sarcosinate, betaine, sodium lauroyl glutamate, sodium cocoyl methyl taurate.
In some embodiments of the application, the sweetener is one or more of sodium saccharin, sucralose, xylitol, erythritol, steviol glycosides.
In some embodiments of the application, the preservative is one or more of sodium benzoate, parabens and salts thereof, polyols.
In some embodiments of the application, the stabilizer is one or more of pyrophosphate and phosphate.
In some embodiments of the application, the light yellow main paste preparation step 1 is added with saccharin sodium with the mass fraction of 0.1%, sodium benzoate with the mass fraction of 0.3%, sodium pyrophosphate with the mass fraction of 0.3%, dipotassium glycyrrhizinate with the mass fraction of 0.2% and arginine with the mass fraction of 0.8%.
In some embodiments of the application, the light yellow main paste preparation step 2 comprises weighing 50% of sorbitol, 3% of PEG400 and 8% of glycerin by mass.
In some embodiments of the present application, 20% hydrated silica, 0.3% xanthan gum, 0.6% polyquaternium-22, 0.3% guar gum, 0.5% chitosan quaternary ammonium salt, and 0.4% holly bark extract are weighed in the light yellow main paste preparation step 3.
In some embodiments of the present application, the light yellow main paste preparation step 4 comprises dissolving 0.1% of cooling agent and 0.05% of rosemary extract in 1% of essence.
In some embodiments of the application, the light yellow main paste preparation step 5 comprises 2.0% of K12, 0.5% of sodium lauroyl sarcosinate and 0.5% of betaine by mass.
In some embodiments of the application, the green auxiliary paste preparation step 2 is added with saccharin sodium 0.1%, sodium benzoate 0.3%, sodium pyrophosphate 0.3%, dipotassium glycyrrhizinate 0.2% and arginine 0.8% by mass.
In some embodiments of the application, the green auxiliary paste preparation step 3 comprises weighing 50% of sorbitol, 3% of PEG400 and 8% of glycerin by mass.
In some embodiments of the present application, the green auxiliary paste preparation step 4 comprises weighing 20% hydrated silica, 0.3% xanthan gum, 0.6% polyquaternium-22, 0.3% guar gum, and 0.4% cortex Ilicis Rotundae extract.
In some embodiments of the present application, the green auxiliary paste preparation step 5 comprises dissolving 0.1% cooling agent and 0.05% rosemary extract in 1% essence.
In some embodiments of the application, the green auxiliary paste preparation step 6 comprises 2.0% of K12, 0.5% of sodium lauroyl sarcosine and 0.5% of betaine by mass.
In some embodiments of the present application, the mass fraction of the weighed deionized water in the blue auxiliary paste preparation step 1 is 0.5% to dissolve 0.1% of sodium guaiacol sulfonate.
In some embodiments of the application, the blue auxiliary paste preparation step 2 is added with saccharin sodium 0.1%, sodium benzoate 0.3%, sodium pyrophosphate 0.3%, dipotassium glycyrrhizinate 0.2% and arginine 0.8% by mass.
In some embodiments of the application, the mass fraction of the blue auxiliary paste in the preparation step 3 is 50% of sorbitol, 3% of PEG400 and 8% of glycerin.
In some embodiments of the present application, 20% hydrated silica, 0.3% xanthan gum, 0.6% polyquaternium-22, 0.3% guar gum, 0.4% holly bark extract are weighed in the blue auxiliary paste preparation step 4.
In some embodiments of the present application, the green auxiliary paste preparation step 5 comprises dissolving 0.1% cooling agent and 0.05% rosemary extract in 1% essence.
In some embodiments of the present application, the blue auxiliary paste preparation step 6 comprises 2.0% of K12, 0.5% of sodium lauroyl sarcosine and 0.5% of betaine by mass.
In some embodiments of the application, the preparation method further comprises a filling step of filling the three-color paste into a toothpaste tube together according to the mass ratio of light yellow main paste to green auxiliary paste to blue auxiliary paste=8-5:1-2.5:1-2.5 to obtain the Chinese herbal medicine three-color strip toothpaste.
In some embodiments of the application, the mass ratio of the yellowish main paste to the green subsidiary paste to the blue subsidiary paste=7:1.5:1.5 or the mass ratio of the yellowish main paste to the green subsidiary paste to the blue subsidiary paste=6:2:2 in the filling step.
Example 1
The light yellow main paste process comprises the following steps:
1. Weighing deionized water (the rest is a proper amount of a flushing container), sequentially adding saccharin sodium with the mass fraction of 0.1%, sodium benzoate with the mass fraction of 0.3%, sodium pyrophosphate with the mass fraction of 0.3%, dipotassium glycyrrhizinate with the mass fraction of 0.2% and arginine with the mass fraction of 0.8%, and uniformly stirring and dissolving for later use;
2. weighing 50% of sorbitol, 3% of PEG400 and 8% of glycerol for later use;
3. Weighing 20% of hydrated silica, 0.3% of xanthan gum, 0.6% of polyquaternium-22, 0.3% of guar gum, 0.5% of chitosan quaternary ammonium salt and 0.4% of cortex rotundae officinalis extract according to mass fraction, and uniformly premixing in a stainless steel pot;
4. Dissolving 0.1% of cooling agent and 0.05% of herba Rosmarini officinalis extract in 1% of essence;
5. Weighing 2.0% of K12, 0.5% of sodium lauroyl sarcosinate and 0.5% of betaine for standby;
6. adding the sample in the step 2 into an ointment making pot, then adding the sample in the step 1, flushing a container with the rest deionized water, and stirring for 3min by a vacuum scraper;
7. adding the sample in the step 3, starting a scraper, stirring until no obvious dry powder exists, starting vacuum, and starting timing for 10min when the vacuum reaches more than-0.092 MPa;
8. The samples added in 4 and 5 are stirred for 30min by a vacuum scraper (the vacuum is not higher than-0.094 MPa);
9. Turning off the scraping plate, stirring, and vacuum degassing for more than 10min until no obvious bubbles exist;
10. And (5) discharging and detecting.
Green auxiliary paste process:
1. weighing 0.5% of deionized water and dissolving 0.05% of sodium copper chlorophyllin;
2. weighing deionized water (the rest is a proper amount of a flushing container), sequentially adding saccharin sodium with the mass fraction of 0.1%, sodium benzoate with the mass fraction of 0.3%, sodium pyrophosphate with the mass fraction of 0.3%, dipotassium glycyrrhizinate with the mass fraction of 0.2% and arginine with the mass fraction of 0.8%, and uniformly stirring and dissolving for later use;
3. Weighing 50% of sorbitol, 3% of PEG400 and 8% of glycerol for later use;
4. Weighing 20% of hydrated silica, 0.3% of xanthan gum, 0.6% of polyquaternium-22, 0.3% of guar gum and 0.4% of cortex rotundae officinalis extract according to mass fraction, and uniformly premixing in a stainless steel pot;
5. Dissolving 0.1% of cooling agent and 0.05% of herba Rosmarini officinalis extract in 1% of essence;
6. weighing 2.0% of K12, 0.5% of sodium lauroyl sarcosinate and 0.5% of betaine for standby;
7. Adding the sample in the step 3 into an ointment making pot, adding the sample in the step 2 and the step 1, flushing a container with the rest deionized water, and stirring for 3min by a vacuum scraper;
8. adding the sample in the step 4, starting a scraper, stirring until no obvious dry powder exists, starting vacuum, and starting timing for 10min when the vacuum reaches more than-0.092 MPa;
9. adding the samples in the step 5 and the step 6, starting a vacuum scraper and stirring for 30min (the vacuum is not higher than-0.094 MPa);
10. turning off the scraping plate, stirring, and vacuum degassing for more than 10min until no obvious bubbles exist;
11. And (5) discharging and detecting.
Blue auxiliary paste process:
1. Weighing 0.5% deionized water to dissolve 0.1% guaiac sodium azulene sulfonate;
2. weighing deionized water (the rest is a proper amount of a flushing container), sequentially adding saccharin sodium with the mass fraction of 0.1%, sodium benzoate with the mass fraction of 0.3%, sodium pyrophosphate with the mass fraction of 0.3%, dipotassium glycyrrhizinate with the mass fraction of 0.2% and arginine with the mass fraction of 0.8%, and uniformly stirring and dissolving for later use;
3. Weighing 50% of sorbitol, 3% of PEG400 and 8% of glycerol for later use;
4. Weighing 20% of hydrated silica, 0.6% of polyquaternium-22, 0.3% of xanthan gum, 0.3% of guar gum and 0.4% of cortex rotundae officinalis extract according to mass fraction, and uniformly premixing in a stainless steel pot;
5. Dissolving 0.1% of cooling agent and 0.05% of herba Rosmarini officinalis extract in 1% of essence;
6. weighing 2.0% of K12, 0.5% of sodium lauroyl sarcosinate and 0.5% of betaine for standby;
7. Adding the sample in the step 3 into an ointment making pot, adding the sample in the step 2 and the step 1, flushing a container with the rest deionized water, and stirring for 3min by a vacuum scraper;
8. adding the sample in the step 4, starting a scraper, stirring until no obvious dry powder exists, starting vacuum, and starting timing for 10min when the vacuum reaches more than-0.092 MPa;
9. adding the samples in the step 5 and the step 6, starting a vacuum scraper and stirring for 30min (the vacuum is not higher than-0.094 MPa);
10. turning off the scraping plate, stirring, and vacuum degassing for more than 10min until no obvious bubbles exist;
11. And (5) discharging and detecting.
Filling, namely filling the paste with the three colors into a toothpaste tube together according to the mass ratio of light yellow main paste to green auxiliary paste to blue auxiliary paste=11:4:4 to obtain the Chinese herbal medicine tricolor strip toothpaste.
Test example 1 quality assessment of toothpaste
The consistencies, specific gravities, foam amounts and pH values of light yellow main paste, green auxiliary paste and blue auxiliary paste in the three-color strip toothpaste are detected, and whether all indexes meet the national standard and the detection range of the conventional industry is determined, wherein the consistencies are 9-15 mm (instant) and 12-18 mm (the next day), the specific gravities are 1.2-1.5, the foam amounts are 100-150 mm, and the pH value is 5.5-10.0.
1. Experimental apparatus and device
Consistometer is used to measure the consistency of toothpaste with an accuracy of 1mm.
The specific gravity bottle has a capacity of 50mL and is used for measuring the specific gravity of the toothpaste.
The foam amount measuring device comprises a 250mL measuring cylinder with a plug (the scale is accurate to 1 mm) and a constant-temperature water bath kettle (the temperature is controlled to be 25+/-2 ℃).
The pH meter has the accuracy of 0.01 and is used for measuring the pH value of the toothpaste.
The electronic balance has the precision of 0.0001g and is used for weighing the weight of the gravity bottle and the sample.
2. Experimental step (1) consistency detection
The method comprises the steps of placing a consistometer on a horizontal table in advance, calibrating an instrument to ensure that a pointer points to a zero scale, taking a proper amount (about 50 g) of toothpaste samples to be measured (light yellow main paste, green auxiliary paste and blue auxiliary paste are respectively carried out), placing the toothpaste samples into a beaker, uniformly stirring the toothpaste with a glass rod to avoid introducing bubbles, carefully filling the stirred toothpaste into a sample container of the consistometer, scraping off superfluous toothpaste on the surface of the toothpaste by the glass rod after filling the toothpaste, flattening the surface of the sample, rapidly and lightly placing a plunger of the consistometer on the surface of the sample to ensure that the plunger is in full contact with and vertical to the surface of the sample, loosening the plunger, starting a stopwatch at the same time, and reading the sinking distance of the plunger after 30 seconds, namely the instant consistomy in mm. Each sample was measured 3 times in duplicate and the average was taken as the immediate consistency of the sample.
The next day of consistency measurement, namely placing the processed toothpaste sample (filled in a consistometer sample container and scraped off the surface) in a laboratory environment (the temperature is 25+/-2 ℃ and the relative humidity is 50+/-5%) according to the method for measuring the instant consistency, standing for 24 hours, after 24 hours, lightly placing a plunger of the consistometer on the surface of the sample again, ensuring that the plunger is completely contacted and vertical to the surface of the sample, loosening the plunger, and reading the sinking distance of the plunger after 30 seconds of timing, namely the next day of consistency. Each sample was also measured 3 times in duplicate and averaged.
(2) Specific gravity detection
The calibration of the gravity bottle comprises weighing the weight of the gravity bottle with an electronic balance, namely m 0, to the accuracy of 0.0001g, filling distilled water (the temperature of the distilled water is controlled to be 25+/-2 ℃), covering the bottle stopper to overflow redundant water from capillaries of the bottle stopper, wiping water outside the gravity bottle by filter paper, weighing the weight of the gravity bottle filled with distilled water again, namely m 1, to the accuracy of 0.0001g, and calculating the volume V= (m 1-m0)/p of the gravity bottle according to the density ρ (0.9970 g/mL) of the distilled water at 25 ℃.
And (3) measuring the specific gravity of the sample, namely pouring distilled water in the specific gravity bottle, flushing the specific gravity bottle with a toothpaste sample to be measured (light yellow main paste, green auxiliary paste and blue auxiliary paste are respectively carried out) for 2-3 times, filling the toothpaste sample, compacting the sample with a glass rod, removing bubbles to enable the surface of the sample to be flush with a bottle opening, covering the bottle stopper, wiping the outside of the specific gravity bottle with filter paper, weighing the mass of the specific gravity bottle filled with the toothpaste sample, and marking as m 2, and accurately reaching 0.0001g.
Calculate the specific gravity of the toothpaste sample specific gravity= (m 2-m0)/V, measure 3 times each sample repeatedly, take the average as specific gravity of the sample.
(3) Foam quantity detection
The preparation method comprises the steps of adjusting a constant-temperature water bath kettle to 25+/-2 ℃, placing a 250mL measuring cylinder with a plug into the water bath kettle for preheating for 10 minutes, weighing 2.0g (accurate to 0.01 g) of toothpaste sample to be measured (light yellow main paste, green auxiliary paste and blue auxiliary paste are respectively carried out), placing into the preheated measuring cylinder with the plug, and adding 80mL of distilled water with the temperature of 25+/-2 ℃.
The method comprises the steps of vibrating to generate foam, covering a measuring cylinder plug, vibrating the measuring cylinder up and down for 30 times at a speed of 2 times per second, wherein the vibration amplitude is 30-40cm, immediately and vertically placing the measuring cylinder in a constant-temperature water bath kettle after vibration is finished, standing for 1 minute, then reading the height of the foam, wherein the unit is mm, repeatedly measuring each sample for 3 times, and taking the average value as the foam quantity of the sample.
(4) PH detection
The pH meter calibration comprises the steps of turning on a power supply of the pH meter, preheating for 30 minutes, and carrying out two-point calibration on the pH meter by using a standard buffer solution (phosphate buffer solution pH=6.86 and borax buffer solution pH= 9.18,25 ℃ to ensure that the calibration error is within +/-0.01).
The method comprises the steps of taking a proper amount of toothpaste samples to be measured (light yellow main paste, green auxiliary paste and blue auxiliary paste are respectively carried out), putting the toothpaste samples into a beaker, adding a small amount of distilled water (about 50 mL), stirring uniformly by using a glass rod to prepare a toothpaste solution, washing an electrode of a pH meter with distilled water, sucking water on the surface of the electrode with filter paper, inserting the electrode into the toothpaste solution, reading the pH value to be accurate to 0.01 after the reading number is stable, repeatedly measuring each sample for 3 times, and taking the average value as the pH value of the sample.
3. Data recording and processing
The measurement data of each sample of the three-color bar toothpaste of Chinese herbal medicine under each detection index is recorded according to the experimental design, as shown in the following table 1.
Table 1 three-color bar toothpaste test index and results
| Name of product | Instant consistency mm | Consistency of mm the next day | Specific gravity | Foam amount mm | pH |
| Light yellow main ointment | 15 | 18 | 1.327 | 130 | 7.80 |
| Green auxiliary paste | 15 | 18 | 1.317 | 140 | 7.72 |
| Blue auxiliary paste | 12 | 15 | 1.323 | 140 | 7.70 |
The consistencies, specific gravities, foam amounts and pH values of the light yellow main paste, the green auxiliary paste and the blue auxiliary paste in the trichromatic color strip toothpaste all accord with the national standard and the detection range of the industry convention, wherein the pH values and the foam amounts are excellent, and the consistencies and the specific gravities are in reasonable intervals.
Test example 2 toothpaste antibacterial experiment scheme
The antibacterial activity of the trichromatic toothpaste, the monochromatic toothpaste and the blank toothpaste is evaluated by adopting an in-vitro agar diffusion method (K-B method) measurement method and taking common pathogenic bacteria (streptococcus mutans, porphyromonas gingivalis and candida albicans) in the oral cavity as targets.
1. Experimental materials and apparatus
Test sample (I)
Trichromatic strip toothpaste (mixed paste, recorded as S group);
pale yellow main paste (noted as group a);
green auxiliary paste (noted as group B);
blue auxiliary paste (noted as group C);
Blank paste (blank paste solution without dipotassium glycyrrhizinate, arginine, chitosan quaternary ammonium salt, cortex Ilicis Rotundae extract, sodium copper chlorophyll, sodium guaiacol sulfonate and herba Rosmarini officinalis extract components, marked as group D).
(II) target strains
Streptococcus mutans (Streptococcus mutans, ATCC 25175, cariogenic bacteria);
Porphyromonas gingivalis (Porphyromonas gingivalis, ATCC 33277, periodontitis pathogenic bacteria);
candida albicans (ATCC 10231, oral fungi).
(III) Medium
Brain heart infusion agar (BHIAgar, used for bacterial culture);
save agar (SDAAgar, used for fungal culture).
2. Testing method and procedure
Sample pretreatment
Dissolving paste, taking 1.0g of toothpaste sample (S/A/B/C group), adding 9mL of sterile physiological saline, vortex mixing, centrifuging at 3000rpm for 10min, and collecting supernatant as test solution (if paste contains insoluble particles, 0.45 μm sterile filter membrane is needed).
Negative control an equal amount of blank paste (group D) was taken and a matrix solution was prepared as described above.
The concentration calibration ensures that the concentration of the paste in the test solution is 10% (w/v), i.e. 1g paste corresponds to 10mL of solution (if the volume changes after centrifugation, physiological saline needs to be added for adjustment).
Preparation of (II) bacterial suspension
Bacteria (Streptococcus mutans and Porphyromonas gingivalis) are recovered and inoculated into BHI liquid culture medium, and cultured at 37 ℃ for 24 hours in a constant temperature incubator, and the concentration of the bacteria is regulated to 1X 10 8 CFU/mL by using 0.85% physiological saline.
Fungus (Candida albicans) is inoculated in SDA liquid culture medium, cultured at 30deg.C in a constant temperature incubator for 48h, and the concentration of the fungus is adjusted to 1×10 6 CFU/mL with 0.85% physiological saline.
Preparation of agar plates
Under aseptic conditions, molten BHI agar (bacterial) or SDA agar (fungal) is poured into sterile petri dishes (90 mm diameter) and about 4mm thick (about 15-20mL per dish) and allowed to solidify for use.
(IV) determination of zone of inhibition (oxford cup method)
And (3) coating a bacterial film, namely dipping a bacterial suspension by using a sterile cotton swab, uniformly coating the surface of the agar for 3 times, rotating 90 degrees each time, ensuring uniform bacterial liquid distribution, and standing for 5 minutes to dry the surface.
Sample application, namely placing oxford cups in the centers of the plates by using sterile forceps, lightly pressing to enable the oxford cups to be closely attached to agar, adding 20 mu L of test solution (S/A/B/C/D groups) into each oxford cup, taking care of avoiding bubbles and overflowing, and arranging 3 parallel plates for each group of strains, wherein the total number of the plates is 18.
Culturing and observing bacteria for 48h at 37 ℃ in an inverted mode (streptococcus mutans needs an anaerobic environment and porphyromonas gingivalis needs an anaerobic incubator), and fungi for 72h at 30 ℃ in an inverted mode, and observing the formation of a bacteriostasis ring.
Data recording and processing, namely measuring the vertical distance from the center of the oxford cup to the edge of the inhibition zone (accurate to 0.1 mm) by using a vernier caliper, wherein the diameter of the inhibition zone is 2 times of the measured value (comprising the diameter of the oxford cup, and the diameter of the inhibition zone is 6 mm), if partial area bacterial growth is close to the oxford cup, the minimum diameter of complete sterile growth is taken as the standard, and if the edge is blurred, the edge of a visible transparent area is calculated, and the data recording and processing are as shown in the following table 2.
TABLE 2 diameter of inhibition zone
| Test set | Streptococcus mutans (mm) | Porphyromonas gingivalis (mm) | Candida albicans (mm) |
| Group D | 6.0±0.0 | 6.0±0.0 | 6.0±0.0 |
| S group | 27.5±1.2 | 26.8±1.0 | 23.3±0.9 |
| Group A | 9.2±0.8 | 8.5±0.7 | 6.8±0.6 |
| Group B | 10.5±1.0 | 10.8±0.9 | 9.2±0.8 |
| Group C | 9.8±0.9 | 8.5±0.6 | 6.9±0.5 |
The group D blank control is 6mm, namely the diameter of the oxford cup, and no antibacterial activity exists, so that the effective antibacterial activity is larger than the diameter of a antibacterial circle by 6mm (namely the diameter of the oxford cup), and the larger the diameter of the antibacterial circle is, the stronger the antibacterial activity is. The diameters of the inhibition zones of the A group (light yellow main paste), the B group (green auxiliary paste) and the C group (blue auxiliary paste) in the single-color paste are almost consistent with each other on three pathogenic bacteria (streptococcus mutans, porphyromonas gingivalis and candida albicans), which indicate that antibacterial activity exists, the three-color paste is related to natural antibacterial components such as dipotassium glycyrrhizinate, arginine, holly bark extract, rosemary extract, sodium copper chlorophyllin and guaiac sodium sulfonate, and the diameters of the inhibition zones of the S group (trichromatic strip toothpaste) on the three pathogenic bacteria (streptococcus mutans, porphyromonas gingivalis and candida albicans) are obviously larger than those of each single-color group (A group/B group/C group), and the multi-component combination generates a synergistic antibacterial effect, so that the three-color strip toothpaste of the traditional Chinese medicine has more excellent inhibition effect on common pathogenic bacteria in the oral cavity.
Test example 3 anti-inflammatory detumescence test
The difference of the effects of the trichromatic toothpaste, the monochromatic toothpaste and the blank toothpaste on the anti-inflammatory and detumescence of mice is tested.
1. Sample to be tested (example 1)
Trichromatic strip toothpaste (mixed paste, recorded as S group);
pale yellow main paste (noted as group a);
green auxiliary paste (noted as group B);
blue auxiliary paste (noted as group C);
Blank paste (blank paste solution without dipotassium glycyrrhizinate, arginine, chitosan quaternary ammonium salt, cortex Ilicis Rotundae extract, sodium copper chlorophyll, sodium guaiacol sulfonate and herba Rosmarini officinalis extract components, marked as group D).
2. Testing method and procedure
Dosage of experiment group (S group, A group, B group, C group) and blank group (D group) were 5g/kg,1 time/day, and the administration method was coating administration.
SPF-grade KM mice, 18-22 g, are male, and are randomly divided into experimental groups (S group, A group, B group and C group) and blank control groups (D group), wherein the total number of the mice is 5, and each group comprises 10 mice.
Pre-inflammatory preparation pre-inflammatory mice were dehaired (about 3.0 cm. Times.3.0 cm) on their abdomen, avoiding skin damage.
Molding and administration, wherein 1mL or 1g (about 2.5cm multiplied by 2.5 cm) of belly is coated for 7d, and 7d is coated, wherein 0.5h is carried out after the last administration, 0.02mL of 100% dimethylbenzene is coated on the two sides of the left ear of each mouse, the right ear is used for comparison, the animals are killed after 2h of inflammation, two ears are cut off along the auricle baseline, discs of the same part of the ears are punched by a puncher with the diameter of 9mm, and an electronic analytical balance is used for weighing (accurate to 0.1 g), so as to calculate the swelling degree and the swelling inhibition rate.
3. Test results
According to the formula, the swelling degree=left-right ear weight, the swelling inhibition ratio = (blank swelling degree-experiment swelling degree)/blank swelling degree×100% were calculated to obtain the test results of the swelling degree and swelling inhibition ratio of the mouse ear, as shown in table 3 below.
TABLE 3 xylene-induced inflammatory ear swelling degree and swelling inhibition ratio
Note that P <0.05 compared to the placebo group
Compared with the D group, the swelling inhibition rate of the S group (trichromatic strip toothpaste) is up to 90.6%, which is obviously higher than that of the A/B/C group (single-color paste, the swelling inhibition rate is less than 25%), the mixed components have synergistic anti-inflammatory effects, the dipotassium glycyrrhizinate, arginine, chitosan quaternary ammonium salt, rosemary extract and holly bark extract in the toothpaste are main anti-inflammatory components, the anti-inflammatory and detumescence effects of the compounded trichromatic strip toothpaste are better than those of single components and most of the contrast, the anti-inflammatory and detumescence effects are obviously enhanced by about 3.6 times, and the synergistic advantages of the multi-component compound anti-inflammatory components are proved, so that the traditional Chinese medicine trichromatic strip toothpaste has more excellent anti-inflammatory and detumescence effects on common pathogenic bacteria of the oral cavity, and has the potential of being developed into functional oral care products.
Test example 4 enamel remineralization and pore repair test
The effect differences of the trichromatic toothpaste, the monochromatic toothpaste and the blank toothpaste on tooth enamel remineralization and pore repair were tested.
1. Experimental materials
Blank toothpaste: basic formula toothpaste (without chitosan quaternary ammonium salt);
Trichromatic strip toothpaste (mixed paste, recorded as S group);
pale yellow main paste (noted as group a);
green auxiliary paste (noted as group B);
blue auxiliary paste (noted as group C);
Blank paste (blank paste solution without dipotassium glycyrrhizinate, arginine, chitosan quaternary ammonium salt, cortex Ilicis Rotundae extract, sodium copper chlorophyll, sodium guaiacol sulfonate and herba Rosmarini officinalis extract components, marked as group D).
2. Experimental procedure
The sample is mainly prepared by grinding 30 teeth of an isolated human body, preparing into 1mm thick enamel sheets, and performing ultrasonic cleaning to obtain undemineralized enamel sheets for standby, wherein the undemineralized enamel sheets are 1.00+/-0.05 in measured hydroxyapatite characteristic peak intensity (relative value), and the SEM observation result shows that the surface is smooth and has no pores.
The demineralization treatment, namely, the non-demineralized enamel sheets are evenly divided into 5 groups, each group of 5 sheets are immersed into a demineralized liquid (pH 4.5) of 0.1mol/L lactic acid and 0.01mol/L calcium chloride, and the shaking is carried out for 6 hours at 37 ℃.
The group intervention is that each group of tooth enamel sheets after the ore removal treatment is respectively placed in a centrifuge tube containing a mixed solution of corresponding toothpaste (2 g) and artificial saliva (20 mL), and is subjected to shaking culture for 7d at 37 ℃ in a constant-temperature shaking incubator, and liquid is changed every 2 d.
The detection analysis comprises the steps of determining the characteristic peak intensity of the hydroxyapatite by an FT-IR spectrometer, quantifying the mineral content, observing the restoration condition of enamel surface pores by an SEM microscope, and obtaining experimental data as shown in the table 4 below.
TABLE 4 characteristic peak intensities of hydroxyapatite of enamel sheets and SEM observations
Experimental results show that compared with the group D (blank toothpaste), the group S and the group A containing chitosan quaternary ammonium salt have obvious improvement on the characteristic peak intensity of the dental enamel hydroxyapatite and the repair condition of the dental enamel surface pores, and compared with the group D, the group B and the group C have no obvious change on the characteristic peak intensity of the dental enamel hydroxyapatite and the repair condition of the dental enamel surface pores.
Further analysis shows that the improvement effect of the characteristic peak intensity of the hydroxyapatite of the dental enamel of the group S is at least 2 times that of the dental enamel of the group A, and the surface pores of the dental enamel of the group S are almost completely repaired by combining with SEM observation results, while more pits still exist in the group A, and the repair conditions of the group B and the group C are not obviously different from those of the group D. Therefore, the chitosan quaternary ammonium salt can effectively promote the remineralization of the tooth enamel and the restoration of the surface pores of the tooth enamel, and compared with the monochromatic toothpaste (A group, B group and C group), the Chinese herbal medicine trichromatic strip toothpaste (S group) has obviously better effect on promoting the remineralization of the tooth enamel and the restoration of the pores. The synergistic effect between the compounding of various components and the chitosan quaternary ammonium salt can obviously promote the effects of promoting the remineralization of enamel and the restoration of pores, and the effectiveness and the advantages of the Chinese herbal medicine trichromatic strip toothpaste in the aspect are proved.
According to the Chinese herbal medicine trichromatic color bar toothpaste and the preparation method thereof, the trichromatic color bar paste toothpaste is obtained under the condition that exogenous pigments are not added, and the toothpaste can realize the composite effects of freshening breath, inhibiting dental plaque and oral pathogenic bacteria, resisting inflammation and detumescence, resisting sensitization, relieving gingivitis, promoting healing and repairing of recurrent oral ulcer, promoting remineralization of dental enamel and pore repair, has strong efficacy cooperativity, stability of paste and higher use safety.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present application. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the application. Thus, the present application is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
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