CN120818557A - Application of TaSIZ1 protein and its encoding gene in regulating plant drought resistance - Google Patents

Abstract

The present invention discloses the application of TaSIZ1 protein and its encoding gene in regulating plant drought resistance, which belongs to the field of biotechnology. The technical problem solved by the present invention is how to regulate the drought resistance of plants. The TaSIZ1 protein disclosed in the present invention is TaSIZ1‑3A, TaSIZ1‑3B and/or TaSIZ1‑3D protein, and the sequences of TaSIZ1‑3A, TaSIZ1‑3B and TaSIZ1‑3D protein are SEQ ID No. 2, SEQ ID No. 5, and SEQ ID No. 8, respectively. Experiments have shown that knocking out or overexpressing the TaSIZ1 gene in plants can change the drought resistance of plants. The TaSIZ1 protein of the present invention and its encoding gene can regulate the drought resistance of plants, and have great production and application potential.

Description

TaSIZ1 protein and application of coding gene thereof in regulation and control of drought resistance of plants

Technical Field

The invention belongs to the technical field of biology, and particularly relates to TaSIZ protein and application of a coding gene thereof in regulating drought resistance of plants.

Background

As one of the most important staple food crops, sustainable production of wheat is critical to guaranteeing food safety. However, water resource shortage and exacerbation of drought stress severely affect wheat yield. Therefore, the related genes of the drought resistance of the wheat are excavated and utilized, and a foundation is laid for improving the drought resistance of the wheat and breeding drought-resistant wheat varieties.

Disclosure of Invention

The invention aims to solve the technical problem of regulating drought resistance of plants.

To solve the above technical problems, the present invention provides, first, any one of the following applications of proteins or substances regulating the content or activity of the proteins:

D1 D2) preparing a product for regulating and controlling the drought resistance of the plant, D3) cultivating a drought resistance changing plant, D4) preparing a product for cultivating the drought resistance changing plant;

the protein is derived from wheat (Triticum aestivum l.), and is named TaSIZ protein, taSIZ1-3A protein, taSIZ1-3B protein and/or TaSIZ1-3D protein:

the TaSIZ A protein is A1), A2) or A3) as follows:

A1 A2) a protein which has 75% of the same identity with A1) and the same function and is obtained by substituting and/or deleting and/or adding one or more amino acid residues of the amino acid sequence shown as SEQ ID No.2 in a sequence table, A3) a fusion protein obtained by connecting labels at the N end or/and the C end of A1) or A2);

the TaSIZ1-3B protein is H1), H2) or H3) as follows:

h1 H2) a fusion protein obtained by substituting and/or deleting and/or adding one or more amino acid residues for the amino acid sequence shown as SEQ ID No.5 in a sequence table, wherein the protein has more than 75% of identity with H1) and the same function, and H3) is obtained by connecting a label at the N end or/and the C end of H1) or H2);

The TaSIZ1-3D protein is I1), I2) or I3) as follows:

i1 The amino acid sequence is protein of SEQ ID No.8, I2) protein which is obtained by substituting and/or deleting and/or adding one or more amino acid residues for the amino acid sequence shown in SEQ ID No.8 in a sequence table, has more than 75 percent of identity with I1) and has the same function, and I3) fusion protein which is obtained by connecting labels at the N end or/and the C end of I1) or I2).

The protein of A2), H2), I2) above, wherein the protein has an identity of 75% or more, 80% or more, 85% or more, 90% or more, 95% or more, 96%, 97%, 98% or 99% identity. Identity refers to the identity of amino acid sequences. The identity of amino acid sequences can be determined using homology search sites on the internet, such as BLAST web pages of the NCBI homepage website. For example, in advanced BLAST2.1, by using blastp as a program, expect values are set to 10, all filters are set to OFF, BLOSUM62 is used as Matrix, gap existence cost, per residue gap cost and Lambda ratio are set to 11,1 and 0.85 (default values), respectively, and identity of a pair of amino acid sequences is searched for and calculated, and then the value (%) of identity can be obtained.

The TaSIZ protein in A2) can be synthesized artificially or can be obtained by synthesizing the coding gene and then biologically expressing.

The coding gene of TaSIZ protein in the above A2) can be obtained by deleting one or several amino acid residues of the codons of the DNA sequence shown in the 217-3006 th site of SEQ ID No.1, the 240-3017 th site of SEQ ID No.4 or the 92-2902 th site of SEQ ID No.7, and/or making one or several base pair missense mutations, and/or attaching a tag coding sequence to the 5 'end and/or the 3' end. Wherein, the DNA molecules shown in the 217 th to 3006 th positions of SEQ ID No.1, the 240 th to 3017 th positions of SEQ ID No.4 and the 92 th to 2902 th positions of SEQ ID No.7 respectively encode TaSIZ proteins shown in SEQ ID No.2, SEQ ID No.5 and SEQ ID No. 8.

A3 The tag may be a polypeptide or protein that is fusion expressed with the protein of interest using in vitro recombinant DNA techniques to facilitate expression, detection, tracking and/or purification of the protein of interest. The tag may be a Poly-Arg, poly-His, FLAG, strep-tag II, c-myc, MBP tag, HA tag, GST tag, and/or SUMO tag, etc.

In the above application, the substance may be any one of the following B1) to B9):

B1 A nucleic acid molecule encoding TaSIZ protein, B2) an expression cassette containing B1) the nucleic acid molecule, B3) a recombinant vector containing B1) the nucleic acid molecule, or a recombinant vector containing B2) the expression cassette, B4) a recombinant microorganism containing B1) the nucleic acid molecule, or a recombinant microorganism containing B2) the expression cassette, or a recombinant microorganism containing B3) the recombinant vector, B5) a transgenic plant cell line containing B1) the nucleic acid molecule, or a transgenic plant cell line containing B2) the expression cassette, B6) a transgenic plant tissue containing B1) the nucleic acid molecule, or a transgenic plant tissue containing B2) the expression cassette, B7) a transgenic plant organ containing B1) the nucleic acid molecule, or a transgenic plant organ containing B2) the expression cassette, B8) a nucleic acid molecule that reduces the protein content or activity, B9) a transgenic plant cell line containing B8) the nucleic acid molecule, a recombinant vector, a recombinant microorganism, a transgenic plant cell line, or a transgenic plant tissue containing B1) the nucleic acid molecule.

Wherein the nucleic acid molecule may be DNA such as cDNA, genomic DNA or recombinant DNA, or RNA such as mRNA or hnRNA.

The nucleotide sequence encoding TaSIZ protein of the present invention can be easily mutated by one of ordinary skill in the art using known methods, such as directed evolution and point mutation. Those artificially modified nucleotides having 75% or more identity to the nucleotide sequence of TaSIZ protein of the present invention are derived from the nucleotide sequence of the present invention and are equivalent to the sequence of the present invention as long as they encode TaSIZ protein and function as TaSIZ protein.

In the application B1), the nucleic acid molecule encoding the TaSIZ A protein is a DNA molecule with the coding sequence of the 217 th to 3006 th positions of SEQ ID No.1, the nucleic acid molecule encoding the TaSIZ A-3B protein is a DNA molecule with the coding sequence of the 240 th to 3017 th positions of SEQ ID No.4, and the nucleic acid molecule encoding the TaSIZ A-3D protein is a DNA molecule with the coding sequence of the 92 th to 2902 th positions of SEQ ID No. 7.

Specifically, in B1), the nucleic acid molecule encoding the TaSIZ A protein can be the DNA molecule shown in the 217 th to 3006 th positions of SEQ ID No.1, the DNA molecule shown in the SEQ ID No.1 or the DNA molecule shown in the SEQ ID No.3, the nucleic acid molecule encoding the TaSIZ A3B protein can be the DNA molecule shown in the 240 th to 3017 th positions of SEQ ID No.4, the DNA molecule shown in the SEQ ID No.4 or the DNA molecule shown in the SEQ ID No.6, and the nucleic acid molecule encoding the TaSIZ A3D protein can be the DNA molecule shown in the 92 th to 2902 th positions of SEQ ID No.7, the DNA molecule shown in the SEQ ID No.7 or the DNA molecule shown in the SEQ ID No. 9.

In the above applications, the expression cassette (TaSIZ gene expression cassette) of B2) comprising a nucleic acid molecule encoding TaSIZ a protein refers to DNA capable of expressing TaSIZ a protein in a host cell, which DNA may include not only a promoter for initiating transcription of TaSIZ a gene, but also a terminator for terminating transcription of TaSIZ a gene. Further, the expression cassette may also include an enhancer sequence.

In B3) above, a recombinant expression vector containing the gene expression cassette may be constructed using a plant expression vector. The plant expression vector can be a Gateway system vector or a binary agrobacterium vector, etc., such as pGWB411、pGWB412、pGWB405、pBin438、pCAMBIA1302、pCAMBIA2301、pCAMBIA1301、pCAMBIA1300、pBI121、pCAMBIA1391-Xa or pCAMBIA1391-Xb. When TaSIZ1 is used to construct a recombinant expression vector, any one of an enhanced, constitutive, tissue-specific or inducible promoter such as cauliflower mosaic virus (CAMV) 35S promoter, ubiquitin gene Ubiqutin promoter (pUbi) and the like may be added before the transcription initiation nucleotide thereof, and they may be used alone or in combination with other plant promoters, and in addition, when the plant expression vector is constructed using the gene of the present invention, enhancers including translation enhancers or transcription enhancers may be used, and these enhancer regions may be ATG initiation codons or adjacent region initiation codons and the like, but must be the same as the reading frame of the coding sequence to ensure proper translation of the entire sequence. The sources of the translational control signals and initiation codons are broad, and can be either natural or synthetic. The translation initiation region may be derived from a transcription initiation region or a structural gene.

Recombinant vectors containing the TaSIZ gene expression cassette can be constructed using existing expression vectors.

In the above applications, the vector may be a plasmid, cosmid, phage or viral vector. The plasmid may specifically be pBUE414,414 vector or pWMB110,110 vector.

B3 The recombinant vector is also a recombinant vector which can over-express TaSIZ1 gene and is prepared by utilizing pWMB110,110 vectors. The recombinant vector can express the cDNA molecules or DNA molecules at the 217 th to 3006 th positions of SEQ ID No.1 in a sequence table. B3 The recombinant vector may specifically be pWMB.about.110-TaSIZ 1. The pWMB110-TaSIZ1 is a recombinant vector obtained by inserting a DNA molecule shown as 217-3003 nucleotide of SEQ ID No.1 into a multiple cloning site by utilizing the BamHI recognition site of pWMB vector.

B8 The TaSIZ 1-reduced content nucleic acid molecule may be sgRNA targeting the TaSIZ 1-encoding gene.

B9 The recombinant vector may be a recombinant vector that can edit TaSIZ genes prepared using CRISPR/Cas9 system. The recombinant vector can transcribe sgrnas that target the nucleic acid molecule of B1). The target sequence of the sgRNA may be positions 1574-1592 and/or 2157-2175 of SEQ ID No.3 (i.e. positions 1579-1597 and/or 2129-2147 of SEQ ID No.6, positions 1404-1422 and/or 1919-1937 of SEQ ID No. 9).

In the above application, the microorganism may be yeast, bacteria, algae or fungi. Wherein the bacterium may be Agrobacterium, such as Agrobacterium tumefaciens EHA105.

In the above applications, none of the transgenic plant cell lines, transgenic plant tissues and transgenic plant organs include propagation material.

In the application, the substance for regulating and controlling the TaSIZ protein content or activity is a substance for improving the TaSIZ protein content or activity, the drought resistance of the plant is improved, and the drought resistance of the cultivated plant is changed into the drought resistance of the cultivated plant;

The substance for regulating and controlling TaSIZ protein content or activity is a substance for reducing TaSIZ protein content or activity, the drought resistance of the plant is reduced, and the drought resistance changing plant is cultivated to reduce the drought resistance.

The invention also provides any one of the following methods:

X1) a method for improving drought resistance of a plant, comprising the steps of enabling TaSIZ protein to be expressed in the plant or improving the content or activity of TaSIZ protein in the plant to improve the drought resistance of the plant, X2) a method for cultivating the plant with improved drought resistance, comprising the steps of enabling TaSIZ protein to be expressed in the plant or improving the content or activity of TaSIZ protein in the plant to obtain a target plant with improved drought resistance, X3) a method for reducing the drought resistance of the plant, comprising the steps of reducing the content or activity of TaSIZ protein in the plant or knocking out a coding gene of TaSIZ protein in the plant or reducing the expression amount of a coding gene of TaSIZ protein in the plant to realize the reduction of the drought resistance of the plant, and X4) a method for cultivating the plant with reduced drought resistance, comprising the steps of reducing the content or activity of TaSIZ protein in the plant or knocking out the coding gene of TaSIZ protein in the plant or reducing the expression amount of the coding gene of TaSIZ protein in the plant to obtain the target plant with reduced drought resistance.

Among the above methods, the methods X1) and X2) are realized by introducing a gene encoding TaSIZ protein into a plant and allowing the gene to be expressed.

In the above method, the coding gene may be the nucleic acid molecule of B1).

Among the above methods, the methods of X3) and X4) can be realized by editing the gene encoding TaSIZ protein so that the function of the protein encoded by the gene is changed.

The editing may be accomplished using a CRISPR/Cas9 approach. Gene editing of the coding gene by a CRISPR/Cas9 method can be achieved by introducing a recombinant vector which codes Cas9 and can transcriptionally target sgRNA of the coding gene into plants to screen for the target plant in which the coding gene is edited.

In one embodiment of the invention, the TaSIZ1-3A genome is deleted for 6 nucleotides (i.e., positions 1576-1578 and 2168-2170 of SEQ ID No. 3), the TaSIZ1-3B genome is inserted for 2 nucleotides (i.e., 1G is inserted between positions 1581-1582 and 1T is inserted between positions 2144-2145 of SEQ ID No. 6) resulting in a frame shift mutation and premature translation termination, and the TaSIZ1-3D genome is deleted for 6 nucleotides (i.e., positions 1406-1408 and 1930-1932 of SEQ ID No. 9).

In one embodiment of the invention, the TaSIZ-3A genome is deleted for 596 nucleotides (i.e., the 1577-2172 position of SEQ ID No.3 is deleted), the TaSIZ1-3B genome is deleted for 29 nucleotides, 1 nucleotide is substituted, 1 nucleotide is inserted (i.e., the 1585-1613 position of SEQ ID No.6 is deleted, nucleotide A at 1582 is substituted for T, and 1A is inserted between 2142-2143 positions) resulting in a frameshift mutation and premature translation termination, and the TaSIZ1-3D genome is deleted for 528 nucleotides (i.e., the 1407-1934 position of SEQ ID No. 9).

In one embodiment of the invention, the TaSIZ-3A genome is deleted for 9 nucleotides, 12 nucleotides are substituted, 14 nucleotides are inserted (i.e. the 1577-1585 th nucleotide of SEQ ID No.3 is deleted, the 2160-2171 th nucleotide GACTGATTACAA is substituted for ACTGATTAAAAG, GGTGAGTGTACTTT is inserted between 2174-2175 th nucleotide) resulting in a frame shift mutation and premature termination of translation, the TaSIZ1-3B genome is deleted for 563 nucleotides (i.e. the 1582-2144 th nucleotide of SEQ ID No. 6) and the TaSIZ1-3D genome is deleted for 528 nucleotides (i.e. the 1407-1934 th nucleotide of SEQ ID No. 9).

The recombinant vector can be introduced into plant cells by conventional biotechnology methods such as Ti plasmid, plant viral vector, direct DNA transformation, microinjection, electroporation, and the like (Weissbach,1998,Method for Plant Molecular Biology VIII,Academy Press,New York,pp.411-463;Geiserson and Corey,1998,Plant Molecular Biology(2nd Edition).).

The plant of interest is understood to include not only the first generation plants in which the TaSIZ protein or its coding gene has been altered, but also their progeny. For the plant of interest, the gene may be propagated in that species, or may be transferred into other varieties of the same species, including particularly commercial varieties, using conventional breeding techniques. The plants of interest include seeds, calli, whole plants and cells.

TaSIZ1 proteins or substances which regulate the protein content or the activity of TaSIZ1 are also within the scope of the invention.

In the present invention, the plant is M1) or M2) or M3) or M4) M1) a monocotyledonous plant, M2) a gramineous plant, M3) a wheat plant, or M4) wheat.

Experiments prove that the drought resistance of the plant can be changed by knocking out or over-expressing TaSIZ gene in the plant, and the TaSIZ protein and the coding gene thereof can regulate and control the drought resistance of the plant, so that the plant has great production and application potential.

The following detailed description of the invention is provided in connection with the accompanying drawings that are presented to illustrate the invention and not to limit the scope thereof. The examples provided below are intended as guidelines for further modifications by one of ordinary skill in the art and are not to be construed as limiting the invention in any way.

Drawings

FIG. 1 shows the relative expression levels of TaSIZ genes in different transgenic wheat lines.

FIG. 2 shows the sequence changes of the gene editing lines.

FIG. 3 is a graph showing the identification of drought resistance phenotype of transgenic wheat lines.

Detailed Description

The experimental methods in the following examples, unless otherwise specified, are conventional methods, and are carried out according to techniques or conditions described in the literature in the field or according to the product specifications. Materials, reagents, instruments and the like used in the examples described below are commercially available unless otherwise specified.

The following examples use SPSS27 statistical software to process the data, and experimental results are expressed as mean ± standard deviation, using independent sample T-test, P <0.05 (x) indicates significant differences, P <0.01 (x) indicates significant differences, and P <0.001 (x) indicates significant differences.

The TaSIZ genes of examples 1 and TaSIZ can regulate and control drought resistance of wheat

In the embodiment, a gene capable of regulating drought resistance is found in Chinese spring of wheat, the name of the gene is TaSIZ gene, and three TaSIZ1 homologous genes are TaSIZ1-3A, taSIZ1-3B, taSIZ1-3D respectively.

In China spring, the full-length cDNA of TaSIZ A gene is shown in SEQ ID No.1, nucleotide numbers 217-3006 are open reading frames, the TaSIZ1-3A protein shown in SEQ ID No.2 is encoded, the genome sequence is shown in SEQ ID No.3, the full-length cDNA of TaSIZ1-3B gene is shown in SEQ ID No.4, nucleotide numbers 240-3017 are open reading frames, the TaSIZ1-3B protein shown in SEQ ID No.5 is encoded, the genome sequence is shown in SEQ ID No.6, the full-length cDNA of TaSIZ1-3D gene is shown in SEQ ID No.7, the nucleotide numbers 92-2902 are open reading frames, the TaSIZ1-3D protein shown in SEQ ID No.8 is encoded, and the genome sequence is shown in SEQ ID No. 9.

1. Preparation of transgenic wheat

Firstly) obtaining transgenic wheat over-expressing TaSIZ A gene

1. Extracting mRNA of Chinese spring wheat, taking the mRNA as a template, and adopting a primer pair consisting of F and R to perform RT-PCR to obtain a PCR amplification product 1 (through sequencing, the PCR amplification product 1 contains DNA molecules shown by 25 th-3378 th nucleotides of SEQ ID No. 1).

F:5′-AGATTTGATTTGCGGCGGAC-3′,R:5′-GGGCATCTCACTCAATATTAGCA-3′。

2. And (2) taking the PCR amplification product 1 obtained in the step (1) as a template, adopting a primer pair consisting of TF1 and TR1 to carry out RT-PCR amplification, and adopting high-fidelity enzyme Pfu to amplify a target gene to obtain a PCR amplification product 2.

TF1:5′-CGACTCTAGAGGATCCATGGGGGACCTGGCCTCC-3′,

TR1:5′-CGGTACCCGGGGATCCCTGCTGTGTCGTTAGTCGAGGC-3′。

In TF1 and TR1, the BamHI cleavage site is underlined, and the sequence preceding (5' side) the underlined is the homology arm (the same fragment as the sequence near the insertion position of vector pWMB band).

3. The vector pWMB was cut with the restriction enzyme BamHI, and the about 10kb vector backbone was recovered to obtain the digested vector 1.

4. The PCR amplified product 2 recovered In the step 2 and the digested vector 1 (10 kb vector backbone) obtained In the step 3 were subjected to homologous recombination by using a seamless ligase (seamless ligase is seamless ligase In-fusion, purchased from TaKaRa (cat: 638947)) and mixing the two, incubating at 50℃for 20min, and subjecting to conventional transformation to obtain a recombinant expression vector pWMB110-TaSIZ1.

Sequencing results show that the recombinant expression vector pWMB-TaSIZ 1 is a recombinant vector obtained by inserting a DNA molecule shown by the 217 th to 3003 rd nucleotides of SEQ ID No.1 into a multiple cloning site by utilizing the BamHI recognition site of the pWMB vector, so that the DNA molecule shown by the 217 th to 3003 rd nucleotides of the SEQ ID No.1 can be correctly expressed, and other nucleotides of the pWMB vector are kept unchanged, and is named as the recombinant expression vector pWMB110-TaSIZ1.pWMB110-TaSIZ1 can express the fusion protein of TaSIZ1-3A protein shown in SEQ ID No.2 and GFP.

5. Transgenic wheat overexpressing TaSIZ A gene was obtained.

Transferring the expression TaSIZ-3A wheat vector (recombinant expression vector pWMB-TaSIZ 1) into a wheat variety Fielder through agrobacterium (agrobacterium EHA 105), obtaining 5 homozygous lines through selfing, taking the obtained 5 homozygous lines of TaSIZ-3A gene over-expressed wheat as strains to be tested, respectively named as OE5, OE6, OE11, OE15 and OE19, detecting the relative expression amount of TaSIZ-3A genes (taking the TUBLIN genes of constitutive expression as reference genes) in T3 generation plants (OE 5, OE6, OE11, OE15 and OE 19) of each strain by qRT-PCR, detecting the expression of TaSIZ-3A genes by using primer pairs consisting of F1 (5'-CGTGCTGTCTTTGTAGATCTCG-3') and R1 (5'-GACCAGTGCAGTTGTCTGAAAG-3'), and taking a small amount of primer pairs consisting of primer F2 (5'-CTGTCACGCCGGATTTCAAC-3') and primer R2 (5'-GCACACAGTCGATATGCTGC-3'), as a drought-resistant result, detecting the expression of TaSIZ-3A genes in the strain to be tested, and taking the strain to be tested as strain TaSIZ, and taking the strain TaSIZ-3A gene over-expressed by the strain to be tested.

Two) TaSIZ Gene-edited wheat was obtained

1. The editing site of the TaSIZ gene was predicted using an online website (http:// www.e-CRISP. Org/E-CRISP /).

2. The target sites of the TaSIZ gene are designed according to the TaSIZ1 genome searching conserved regions of TaSIZ1-3A, taSIZ1-3B, taSIZ1-3D, the two target sites are 1574-1592, 2157-2175 of SEQ ID No.3, 1579-1597, 2129-2147 of SEQ ID No.6, 1404-1422 and 1919-1937 of SEQ ID No.9 respectively, and then four primers, specifically an upstream primer F (5'-aataatggtctcAGGCgTGAGACTGATTACAAATGA-3'), F0 (5'-gTGAGACTGATTACAAATGAgttttagagctagaaatagc-3') and a downstream primer R (5'-ATTATTGGTCTCTAAACACTAATCCGAGCAGACCCG-3') and R0 (5'-ACTAATCCGAGCAGACCCGCGCTTCTTGGTGCC-3') are designed according to the carrier usage instructions.

3. Four primers (TaSIZ 1-MT1T 2F/F0/R0/R) are dissolved and mixed, a target sequence is connected by adopting a side enzyme trimming mode through T4 DNA LIGASE and endonuclease BasI (both are NEB company products), a Golden gate vector construction method is adopted, pCBC-MT1T2 is taken as an intermediate vector, pBUE414 is taken as a final vector, a recombinant vector for editing TaSIZ1 genes, namely a TaSIZ1 gene editing wheat vector, is constructed, and the obtained vector can simultaneously transcribe two sgRNAs of target SEQ ID No.3, 6 and 9.

4. After TaSIZ gene editing wheat vector is introduced into agrobacterium tumefaciens EHA105, wheat field is transformed by agrobacterium-mediated genetic transformation method to obtain gene editing plant, specific primers are synthesized aiming at editing sites, target genes are amplified respectively, and then sequencing and identifying editing conditions are carried out to obtain TaSIZ gene editing transgenic wheat siz1-4-1, siz1-7-1 and siz1-7-3.

The sequence of three (siz 1-4-1, siz 1-7-3) gene editing strain sequence changes are shown in FIG. 2, with a pair of specific primers F (5'-GCTGTCCTTCTCTCCAACGTAC-3') and R (5'-TGACAAAGCCAACGAAGAGT-3') for the TaSIZ-3A genome, a pair of specific primers F (5'-GGCAGTCCTTCTCTACAACGTAG-3') and R (5'-GCACATAATAGTTAGCAAACGAAG-3') for the TaSIZ-3B genome, and a pair of specific primers F (5'-CCTGTCCTTCTCTCCAACATAC-3') and R (5'-AGGCAAAGCCAACGATGAGT-3') for the TaSIZ-3D genome amplified, as shown below:

siz1-4-1: taSIZ1-3A genome lacks 6 nucleotides (i.e., deletions of positions 1576-1578 and 2168-2170 of SEQ ID No. 3), taSIZ1-3B genome inserts 2 nucleotides (i.e., 1G between positions 1581-1582 and 1T between positions 2144-2145 of SEQ ID No. 6) resulting in frame shift mutations and premature termination of translation, taSIZ1-3D genome lacks 6 nucleotides (i.e., deletions of positions 1406-1408 and 1930-1932 of SEQ ID No. 9).

Siz1-7-1: taSIZ1-3A genome deleted 596 nucleotides (i.e., deletion of positions 1577-2172 of SEQ ID No. 3), taSIZ-3B genome deleted 29 nucleotides, 1 nucleotide substitution, 1 nucleotide insertion (i.e., deletion of positions 1585-1613 of SEQ ID No.6, 1A insertion between positions 2142-2143) resulting in frame shift mutation and premature termination of translation, taSIZ-3D genome deleted 528 nucleotides (i.e., deletion of positions 1407-1934 of SEQ ID No. 9).

Siz1-7-3: taSIZ1-3A genome deleted 9 nucleotides, 12 nucleotides, 14 nucleotides inserted (i.e. deletion of nucleotide number 1577-1585 of SEQ ID No.3, 2160-2171 with nucleotide number GACTGATTACAA replaced with ACTGATTAAAAG, insertion GGTGAGTGTACTTT between 2174-2175) resulted in frame shift mutation and premature termination of translation, taSIZ-3B genome deleted 563 nucleotides (i.e. deletion of nucleotide number 1582-2144 of SEQ ID No. 6), taSIZ1-3D genome deleted 528 nucleotides (i.e. deletion of nucleotide number 1407-1934 of SEQ ID No. 9).

2. Drought resistance identification

Wheat to be tested transgenic acceptor variety wheat Fielder (wild type control, WT), over-expressed strains OE11 and OE15, gene editing strains siz1-4-1, siz1-7-1 and siz1-7-3. The experiments were divided into two groups, drought treated and control.

1. After the seeds of wheat to be tested are treated with 1% H ₂O₂ ℃ for 24 hours, sterile water is soaked for germination.

2. Seedlings with consistent germination and growth vigor are picked and planted in the same plastic box (56 cm multiplied by 38cm multiplied by 11 cm), 30 seeds are sown in each strain, the plastic box is buried in an outdoor soil environment, and the height of the plastic box is kept at the same level with the ground.

3. Seedlings grown to the two leaves and one heart stage were drought treated (i.e., watering was stopped), rehydrated when the drought treatment occurred for 15 days (i.e., significant differences in phenotype of wild and transgenic wheat plants), and after rehydration for 3 days, survival was counted, and three independent biological replicates were set. As a control, no drought treatment (normal watering) was used.

The survival rate of seedlings was calculated according to the following formula (%) =the number of surviving plants/the number of planted plants×100%.

As shown in FIG. 3, the growth vigor of the wild type and the rest strains before drought treatment are consistent, and compared with the wild type, the survival rate of the over-expression strains OE11 and OE15 is obviously improved, and the survival rates of the gene editing strains siz1-4-1, siz1-7-1 and siz1-7-3 are obviously reduced after rehydration by drought treatment, which indicates that TaSIZ1 can regulate and control drought resistance of wheat in seedling stage.

The genomic sequences referred to above are as follows:

SEQ ID No.3 (TaSIZ 1-3A genomic sequence, wheat (Triticum aestivum L.))):

ACTCGTACGGCGGCGAAGAAAACCAGATTTGATTTGCGGCGGACGCGTCCGTCCGTCCCCCACCCTTTCCCCCCCTTCCCTTCCCCCCGTCCGGCGGCGGGGGAGCCACAAAACTGAAACCGATCGATCGAACCGATTCACACGCCACCGCCGCCGCCGCCGCCGACCTCCACTGGGTCCAGGGCCGCCGCCGCCGCCGCTGCCTCGACTGGGTCCATGGGGGACCTGGCCTCCACCTGCAAGGTCGGTCCTCATCCGATCCACACACACCCTCTCTCTCTCTCGCTCGCTTCTTTGCTTAGGATCCATTCTCCGGTTCCATCCAGGGGTCCCGTCTAGTAATCTAATCTAATCCTGAGTTGCTCTTCTACTTGTTGGTGATGGCAGGACAAGCTTGCCTACTTCAGGATCAAGGAGCTCAAGGACGTGCTCATCCACCTCTCCCTCCCCAAGCACGGCAAGAAGCAGGTACCGTACTCCTCATCTCATCTCATCTCATCTCCTCTCCTCTCATCTCCATCTTTCCCTCCTACCATACTACCTATCATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATATCCTTGTTCATTACTGCTCTCTAGGAGTAGTACCCATCACTATTTATTCCATCCGTTTTAACTACATTCAATTCAATGGATGGATCCACTCTCTCATGTTTCCAAATTATCTACTACTCTACCACCATCACAACAGCTTCTCATACGTATGCTGCACCAGGTTATATTGTCATCCATCTCCAATCATCTCATCTATGCGTTGTTGTTGCAGGACCTCGTCGACAGGATCCTCGCCCTGCTCTCCGACGACCAAGGTCAGCCGCCCCACTTCTTTCCCAACCCTCTCCGTGCCCGTGCAATGCACCCCTGTCATCAACTGCAAAGCAGCCCCAGCGTTTTTTTTAGGGAAAAAAAATCACCTGCTACTTACTAACTTGCCTTGATATGTATGTGGCTGCCTCAGCCCAATGGCACCTTGGTCGAGGGAGGAAGAACGCTCCCAGCAAGGAGGCGGTGCTCAAAATAGTCGACGACATATACAGGTTAGCTAGCTTCCATGCCATTTCCCACTTGTCCACCCTTCTTTCCTTCCTATTTCAGTGCTACTGGTACTTGGCTTATCACACCATTCATTCATTGCCATAGCAGGAAGATGCAAGTCCACGGTCCTCCTGACCTCGTGGTATCTCCTGCTCAGAGCCAGCTGCCTGTCACGCCGGATTTCAACCGCATCATCAAAGCTAAAAAGGAACAATTGGGCCCTGACTCTGGCTGCCTTTGTGGTCAATCCTTTGTTCTCGGGAATGTGGTCAAGGTGTGCCCATCTGCCCTGCCCTGGCTGTCCTTCTCTCCAACGTACAAAAAATGACCATGTTTTCTACACTTGTTTGATTGTTTGTGTGCTTGCTTCTTCTAGTGCGACGATTGCCAGGTCCAGCAGCATATCGACTGTGTGCTGATTCCAGAGAAGCCTGCAGTGGGTGTCAGACCTGAAGCTCCAGAGCATTACTTTTGTCAGTTGTGCCGACTAATCCGAGCAGACCCGTATGCCCTCCTACAATCCACTTCTATTTGCTATTTCTTTCTGTCATTTGCCATCTTGACAAATATGGTGCACTACTGTTACTGGAAACTTCAAAGCGATTCAGACTATTTGTTGCTTCCAGCATTCAAAGTTAGCATGTCCAACCAAATGGGCACTAATGGTAAACTTAATCATGATCCTTATACATATACAACTCTACTGTTGTAACCTAGGTAGGAATTTAACGAACTTGTCAGGTCGAGAGTTGCATCAACTGTATGACTGATTGGTTGATTCAGACTCCCATTATTTGCCAGTAGGCTGAAAAGGAACCTTATGGATCTTATTTGGAGCTCTCGTGTAGCACTACATGTTGCGCCTTGTCTCTAAAACTGTCTTCTAGTATATCATGACAATATCTGAGGGAACAAGATTTAACCCAATAAGTTTCCTATTGATTCTATACAACTTTGTCTCTATTCCTGCATGGATTGCTTGGAATTGAAATAGTTTCTGCACATTTGCTACAGTGACTATTTTGAACCTGCAGATATTGGATTACTACTGGAAATCCTTTACTACCTGTGAGACTGATTACAAATGATGGGTAAGTGTAACTTTATGGGCAATGTATTCCTTGATAGTCTGGTAATTGCTACACTATAATCATCACTTGCTGAGCATTTCAAATTATATTGGAAGGAGTGACCTTATTTGTAGTAGAATACTCTTCGTTGGCTTTGTCAGTCACACTATTATGTGCTACATTCAGTCCACAGTAATCTTACAGAATTCTCAACCATTTGCAGAGTGAATGTTCCTCAGAGTGTGGAGAGAACTTTTCTACTTACTCGAGCTGAGAGGGAGACTGTTCAGAGAGTGGAATATGATATTCAGGTTTGATTCCCTTTTCCTCTGAGACTGATTACTGTCATCCTTTACATTTCCTTTCCACAAGGACAATGACACTTTAATAATGTCATATGGAAATTATTAGCAGTGTGATGCTGCTTTTTCATAACCTTTTCTAAAGGGGAGGGTGCTATAAAATTAACTGAGTGTGGCATGTTCATGATTGCTTGTCATGTTGTCAAAGGCGAAACTCCTTCAAGAGTACCAAATTGCAGATTTTCTTTTTGTGATTTTTAACCTTTTACATGGTGCTTTTCTCTTTATTATGAATAAGAATAAATGCACTTTCTAACGTTATGTTTGCACAAGGACAAAATTCCTACGTTTATCTCGTGCAGTGCCTGTAGGCCTGGGCTGCTGGAGCGTTATATTCTCGTTATAGGGTTAAACTCCCTAGTTGTTTGTTTGTAAAGACAACACACTAGTAGTTTTTTTTGTGTATGTCATGCAGCATGTTGCTGATGAGGGCATGCTCCTTTGCAGGTTTGGTGTATGCTTCTGAACGACAAAGTTCAGTTCAGGATGCACTGGCCTCAGAATGCAGACTTACAAGTGAACGGTACAGCTTTTGCTTTTCAGCTTTCTAGTACAGTTGAGATCGCAGACATGATTTGTTATATGATTCATGCGGTCCTATTTTTGTGTCCTCAGACTCAGAGTTTGAGTCATTCTATACTCCAAAATAGAGGTAGACATGTAGTATCTCGAAAGAGGCCCTTGAGTCATGCTGTAGTTCATTAGTATCAAGTAGTGAATTTATCAATTTATTTTTTTGAAGATTTTATTTGTTCATTTATAATTTTATATACTGTATGCAGATATGTACAGTGAGCCTTGTTTAGGTGTTGCAATGCATTTATCTTGGTAAATTCTTTTATGAGCAAGGCATGGAGGATCATTCTCAATATTGGTTTCATGTTTTCATCAGGTATACAAGTGCGAGTAGTTCCCAGGCCTAGCACTCAGTTACTAGGGATTAATGGACGGGATGATGGGCCGGTGGTGAGTGCACTCCTATTTCATCGCTAGTGCATCTTAACCAACAGAACTATGTCTATTATCTGTCTTTAGTCATCTTAAAGTTATCAGTTGCTTTTCTTGGCAATTCATTTTTTTTTGAGAAACAATTCAATTTAATTTAATTGTGAGTCCCTTGTAAACAACACATCTGCACAGTTCAGAGCTTTTATGTTTTTGGACTGCTTTGCAGATAACAACCTTTTGCCGAGAAGGACAGAATAAAATAGTTTTATCAAGTGATGATGCCCGACCATTTTGTTTTGGGATCAGAATTGCAAAGAGGAGGACAGTTGACCAGGTTTTTCTCTCTCTCTCCTGTTACTTACCTAGCTGTTGAAGTGAATTGTAGAAGGTGCAAACTTTTCTGATAATAAACATGCCATCATCTATACTAAAACATTACCTTTTCAGGTTCTAAACTTGGTGCCAAAGGAAGCTGATGGCGAGTCTTTTGAGGATTCTCTTGCTCGTGTTTGTCGCTGTCTCAGAGGTGGAAATACTACAGATGATGCTGACAGCGATAGTGATTTGGAAGTGGTTGCCGACTTCTTTCCTGTCAGCCTGCGTTGTCCTGTGAGCATTTCTCTACATATTTCTGTTTTCCCTCTAGTTATGAGATTGCCTTTATTGTTTTGAATTTTAAGAAAGAATTCCATATATGAACAGTATCTTTGTCATTGATCAATGAGCTGGTTGTCTCTTGATACTGTCCTTTTACATGCCACACTTTCCCTCAAGTTAAGAATGACAGACATCCAAAAAAGTTAAGAATTACTGAGTTGACTCCAACCGAGTGGTTATCTAGCTGTATTTGGCTGCCATTATATTAGTATGTCATCACAACGTACAAAATAAGCTTTTCTACAGTGCTTCCTGATATGGAAGCTTCAAGGGTTGAACATTTTTTTATCTGATAATATTTCACAGAATAGTGGATCCCGGATAAGGACTGCGGGAAGGTTCAAGCCTTGTGCTCACATGGGCTCTTTTGATCTGCAAACTTTTGTAGAGCTGAATCAACGGTCACGAAAGGTAAGATTTTATTCCTCCATATCCATAACTAGGATCTCTGCATAATGAACATTGTACTTTGGTCCAACTTGTGTTGGTCCATTTGGCAATGGACGGGGTAATTATGATTGGTTGGTCCATTTAAATGCATACTTTCTTCAGATTGACGTGGCCCTATTTTTAAAGACTTGTTGGTTTTTGCAGTGGCAATGTCCAACATGTCTGAAGAATTATTCTATTGAGAGCTTGATCATTGATCGGTATTTCAACCGGATCGCTTCTCTGGTAGGTTTGAATAAGTCATTTTCATTTTCTGTGTATTGTTAGCAGTTTTATGGTTTTCTTAGACTGTTCCTGTGATTCAGGTTCGGAATTGCGGTGAAGATGTCACTGAGATAGATGTGAAGCCTGATGGCTCTTGGCGTGTGAAGGGTGATGTTGAAGATATAAAATTGTCCCTGTGGCACCTGCCTGACGGTTCCCTGTGTGAACTGAAACAAGACGCTAAACCTGTTGCTGGTGATGTAAAATCTGAAGCTTCAAGAATTGGCAGCAAGGGAAATCTAGGCCTTAATGGATTGTGGGAAGCTAGTAAAGCTGTTGACATAAAGCCCTCAAAGCCTATGAGCAGTAGCCACACTGGAATTTACAGGGATGGGGACTACCTAAGTGTGAGTGAGTGCAGCACGCAAATTGGTGAGATGTACAGAGTTGATGATAGACTACAGCAACAGCTCGAAGATGCAGATGTCATTGTTCTCAGCGACTCCGATGATGACAATGTTGTGACAGTGTCTCCACCAGCTGCCTATAGTGATGTTGGTGGTTTGGGATTTGCTCCCATTTCTGCGCCAGGAGTTGCTGAAAGTTACCAGGAGGGTGGTGTAGTTGGGGGCCTTGGCCTTGATTTGTTCAACGACAACAGCGATATTTTTGACATAACTTCCTGGTCTGCGCAACCCCAACCAGAGCAAGGGTTCAATTTTTTCGGAGCTGATGTTCTACTTGGTTCTCACAATTCATCTGATGCAGCGCCAAGTGCTTATACCCTTGGCTGCCACGCTGGCTCTAGTGACACTTCCATGGTTCGAGATCCTTCTACCTGCCATGTACGCACCAGAAGCTTGGTCGATAACCTGTTGCCTTTCGGCAATGATGATTCTTCTCTGCGGATTTTTCTTCCTATTCAACCATCTGGCGTTCCCGTTCAGGAAGAACGGAATGGACATGACAACATGTCAAATGGGGTCCAGCCTGTTGATGACGATGAAGACGAAGATTGGATATCTCTTACACTTGCGGCGGGTGGAGGTAGTAATGAACAGTCTGAGGCAGCAGATACGGTGAGCACACAAGCACAAATCGCAGTGGAAGAGAGAAGGATGGAACCAGCAGACACGCTGAACCCACAAGCACAAATGACGGTGGAAGAGACAAGGATGGAACCAGCAGATGTGTTGAACCCGCAAGCGCAAATTGCAGTGGAAGAGACAAGGATAGAACCAGCAGATGCGTTGAACCCACAAGCACAGATTGCAGTGGAAGAGAGGACGGAACCAGCAGATGCGTTGAGCCCACAAGCACAAGTTACAGTGGAAGAGAGAAGGATGGAACCAGCAGACATTGTTAACCCACAAGCGCAAATTGCAGTGGAAGAAAGAAGAATGGAACCGGCAGAGACGTCGAACCGACAAGCACAAGCACAGATTGCACTGGAAGAGAGAAGGATGGAACCATTGAGTGATGCTGGTTCGTCTCTTTACTATGAGTTCAGTTTTATCTTTTGTGTTATTTATCCGTTTTTCTCCTGATTGATTAACTCCGCTGATAAACTGATTAAACTGGCCCAATACTTTGTGCCAGTGTTTAGTTGGTTTAGTGTTGACAGCCTTTTGTTTTTCTTTTCCCCGTGTTGTTTGCAGAAGGCTCGCCTCCCAGCTTGAATGATGAGAGGCGTAACAAAGGGAATTCAAAAACAAGGGCTGAAAAGATATTTTCTCCTCCGCGGCAGCCTCGATCCGTCAGGCCTCGACTAACGACACAGCAGTGACGTTGAGTAGTTCTTCTTTCTTACTTTGGATAGCTGTGAAATCATGATGTACTTGTGGCCGATGTAAAGAAGAACCAACATGAATGTTTTCATAGTTTAACCTGATGTATAAACTACAGAAGTTTCAAGTGCTGGAAATGAGGGTGCGCCTGAAGGACATCTGACGGTGAGTACTACTACAGTTTTCCTAAGAATGCAAATTTGCAGTTTTGGCGAATAATGGGAATATTTAGGTTCAAACTGGATTGTTTTTAGATATACCGTTCAGACCATATAGATAGCTACACCTTATGAAGGAAGAAATGTGCACATGGCTAAGTTTTAAGATTAGTTTGCTCTGGCTTTCTGCTTCTCTTTTACTAGGAGATACAGATGGCTTTATGCTTGCCAGCATCGATACGGAAGTATGCTATAATGCTATATAGGTTAACTGTCTTAGTCCATGGATGATATATCATACGTATTGTGGTTTTTGTTGATTCACACGCATAATATATTATGTCCTAAACTGATTTCAGTTTATGAAGTTGAGAGATTCCAAAATAGGCATCTTGTTGCAATGATATCATATGGTAAAAACCGGGAAGCTTTATCCAATATTTTTACATTGTTGCTGTTTAAGCCTGGCACATCATTCATGTAGTCCTTTTTTCTTTTCGCCGCGTGGAGATTCAGCTAGTGATGCATTGAAATTGTTTACCAGGTGATGAAGTGTAATTGATCGGAAAAATAATAATGCACTGACGTGTGTTTGTGTTTTTCAACATTGGTGCAGACATAACAGGAAGTGAGCAAGGAAGGCTTGATAGGTTTTGTGGCAGTCTGCTGCAGCATATTGTACATTTGACCACCAGACTATGTAAAAATATATATGTGCTTTGCCGAAATGGAAGGCAAACAAATAGAGATGTGGAGGTTTGTGTATCATTAAATTGTAATTAGGGGTTGGCTTGTGTGTTGCTAATATTGAGTGAGATGCCCTAGTATACTTTCTACTCTTTCCGGAAATTAAATTATTTTTTCATGTTTACTATTATTACATTCTTTTTTCATGTTTCTTGTGTATGCTTGTTACTCTTTTCCGGAATTTTGTTCATGATTATTATTATTACATTCCATGAAC.

SEQ ID No.6 (TaSIZ 1-3B genomic sequence, wheat (Triticum aestivum L.)):

GTGAATGGGCTGCTGCACACAACACACGGATTCGTACGGCGGCGAAGAAAACCAGATTTGATTTGCAGCCGACGCGTCCGTCCGCCCCACCCAGACTTTCCCCCCGTCCGGCGGCGGGGGAGCCACAAAACTGAAACCGATCGATCGAACCGATTCACACGCCACCGCCGCCGCCGCCGCCGCCGCCGCCGACCTCCACTGGGTCCAGGGCTCGTCCCCTCGCTGCCGCCGCCGGCGCCATGGGGGACCTGGCCTCCACCTGCAAGGTCGGTCCGTCGCCCCTCATCTGATCCACCCTCTCTTGCTTGCTCCTCGCCGGCGATTCACTCTCCGCTTCCGGGGGGCTCCTCTCCTCTTCAGGTCGGTCGTCTAGCTCTTCTGATCTAATCTAATCCTGAGGTTGGTCTTTCTTCTTGTTGTTGGTGGTGGTTTCAGGACAAGCTAGCCTACTTCAGGATCAAGGAGCTCAAGGACGTGCTCATCCACCTCTCCCTCCCCAAGCACGGCAAGAAGCAGGTACCTTGTCCACCTCTCCCTCCTCTCATACTACCTATCATATATCATCCACTCTTGTTGATTACTACTACTACTAGGTAGGAGAGTACCCATCACTATTCCATCCCTTACACATTCAATGGATGGATCCACCACTCTTGTTCCCAAATAACTACTCTACCCACCACGGCAACAGCATCCTCTAAATTGCATCGCCACACAAGTTAATTCAGCTTCTCATATGCATGCTGCACGAGGTTCTCTTGTGCTCCTTATCCAATCATCTGATGTATGTGTTGTTGCAGGAGCTCGTCGACAGGATCCTCGCCCTGCTCTCCGACGACCAAGGTCCGCCGCTCTTCTTTTCCTGAGCCTCTCTGTGGCCCGTGCAATGCACCTCTATCTTCAAAAAACGATGCTACATTTTTCATCCTTGCAAAGCAGCAATAGCCTTTTTTTTAGGAAAAAAAAATCACCTGCTACTTACTAATTCACTTTGGTATGTATGTGGCTGCCTCAGCCCAATGGCATCTCGGTCGAGGGAGGAAGAATGCGCCGAGCAAGGAGGCAGTGGTCAAAATAGTCGACGACATATACAGGTTAGCTAGCTCTCATGCCATTTCCCACTGTCCACACTTCTTTCCTTCCTAGTATTTCAGTGCTATTTATTGGTACTTGGGTTATCACACCATTCATTCATTGCCATAGCAGGAAGATGCAAGTCCACGGTCCTCCTGACCTCGTGGTATCCCAGAGCCAGCTGCCTGTCACGACGGATTTCAACCGCATCATCAAAGCCAAAAAGGAACAATTGGGCCCTGACTCTGGCTGCCTTTGTGGTCAATCCTTTGTTCTCGGGAATGTGGTCAAGGTGTGCCCATCTGCCCTGGCCTGGCAGTCCTTCTCTACAACGTAGAAAAAATGACCATGTTTCCTACACTTGTTTGATTATTTGTGTGCTTGCTTCTTCTAGTGCGACGATTGCCAAGTCCAGCAGCATATGGACTGTGTGCTCATTCCAGAGAAGCCTGCAGTGGGCGTCAGACCTGAAGCTCCAGAGCATTATTATTGTCAGTTGTGCCGACTAATCCGAGCAGACCCGTATGCCCTCGTACAATCCACTTCTATTTGCTATTTCCTTTTGTGATTTTCCATCTTGACAAATATGATGCACTACTGTTACTGGAAACTTCAAAGCGATTCAGACTATTAGTTGCTTCCAGCATTCAAAGTTAGGAGGTCCAACCAAATGGGCACTAACTGTTGTTGAGAACATAAATTTAATCATGATCCTTGTACATATACAGCTCTGCTGTTGTAACCTAGGTAGGGGTTTTAACGAACTTGTCAGCTCGAGAGTTGCATCAACTGTATGACTGATTGGTTGATTCAGACTCCTATAGTTTGCCAGTAGGCTGAAAAGGAACCTTCTCTCTAAAACTGTGTTCTAGTATATCATGACAATATCTGAGGGAACAAGATTTAACCCAATAAGTTTCCTATTGATTCTATACAACTTTGTCTCCATTCCTGCATGGATTGCTTGGAATTTAAATATTTTCTGCACATTTGCTACAGTGACTATTTTGAACCTGCAGATATTGGATTACTATTGGAAACCCTTTACTACCTGTGAGACTGATTACAAATGATGGGTAAGTGTAACTTTATGGGCAATGTATTCCTTGATAGTCTGGTAATTGCTATACTATAATCATCACTTACTGAGCATTTCAAATTATATTGGAAGGAGTGACCTTATTTGTAGTAGTAGAATACTCTTCGTTTGCTAACTATTATGTGCTACATCCAGTCCACAATAATCTTACAGAGTTCTCAACCATTTGCAGAATGAATGTTCCTCAGAGTGTGGATAGAACTTTTCTACTTACTCGAGCTGAGAGGGAGACTGTTCAGAGAGTGGAATATGATATTCAGGTTTGATTCCCTTTTCCTCCAAGACTAATTACTGTCATCCTTTACATTTTTTTTTTCCACAAGGACAATGGCACTTTAATAATGTCATGAAAATTATTAGCAGTGTGATGCTGCTTTGTCATAACCTTTTCTAAAGGGGAGGGTGCTATAAAATTAACTGAGTGGCATGTTCATGATTGCTTGTCATGTTGTCAAAGGCAAAACTCCCAAAAGAGTACCAAATTGCAATTCTATTTTGTGATTTTTAACCTTTTACCTGGTGCTTTTCTCTTGATTATGAATAAATGCACTTTCTAACGTTATGTTTGTACAAGGACAAAATTCTTATGTTTATCTCGTGCAGTGCCTGTAGGCCCGGGCTGCTGGAGCATTATATTCTCGTTATAGGGTTAAACTCCCTAGTTGGTTGTTTGCAAAGACAGCACACTAGTAGTGTTTTTGTGTATGTCATGCAGCATGTCGCTGATGAGGGCATGCTCCTTTGCAGGTTTGGTGTATGCTTCTGAATGACAAAGTTCAGTTCAGGATGCACTGGCCTCAGAATGCAGACTTACAAGTGAACGGTACAGCTTTTGCTTTTCAGCTTTCTATTACAGTTGAGACCACAGACATGATTTGTTATATGATTCATGAGGTTCTATTTTTATGTCCTCAGACTCACAGTTTGATTCATTTTTATACTCCAAAATAGAGGTAGACATATAGTACCTTGAAAGAGGCCCTTGAGTCATGTTGTAGTTCACTAGTATCAAGTAGTGAATTTATCAATTTATTTTTTTAAGTCTTCATTTGTTCATTTATAATTTTATATAGTGTATGCAGATATGTACAGTGAGCGTTGTTTAGGTGTTGTAATGCATTTATCTTGGTAAATTCTTTTATGAGCAAGGCATGGAGGATCATTTTCAATATTGGTTTCATGTTTTCATCAGGTATACAAGTGCGAGTAGTTCCCAGGCCTAGCACTCAGTTACTAGGGATTAATGGACGTGATGATGGGCCGGTGGTGAGTTCACTCCTATTCCATTGCTAGTGCATCTTAACCAACAGAGCATTTGTCTTTAGTCATCTTAAAGTTATCAGTTGCTTTTTTTTGCAATTTAATTTAATTGCGAGTTCCCTTGTAAACAGCACATCTGCACAGTTCAGAACATTTTTTGTTTTTGCACTGCTTTGCAGATAACAACCTTTTGCCGAGAAGGACAGAATAAAATAGTTTTAACAAGTGATGATGCTCGACCATTTTGTTTTGGGATCAGAATCGCGAAGAGGAGGACAGTTGACCAGGTTTTTTTCTTGCCTTTTACTTACCTAGCAATTGTAGTGAATTGTAGAAGGTGCAAACTTTTCTGGCTTGTTTGAGTTGCCCTTTCTATGATTGGATTGCAATCTGAAAAAGAACGATTTGTAAGTGGATCGTAGATGTGGTCACAATAACCGAAACTCTTAATAATATTGCCCTAAATGTTCCTCGATAAACATTAATAAATCAAGTAACTTTTGTAAGCACAATGGTCATTTCCCCCCCTTTATGTTGATCAATTTTTTCAACTCAATAGTTTCACACCCAGATTACTTTCTAACACATGTTTCGTCATATTACTCCCTCTGTAAAGAAATATAAGAGCATTAGTGATCTAAATGCTCTTATATTTCTTTACAGAGGGAGTACTTCTGAAGTTGACTATTTTATGTGATAATAAACATGCCATCGCCTATACTAAAACATTACCTTTTCAGGTTCTAAACTTGGTGCCAAAGGAAGTTGATGGCGAGTCTTTTGAGGATTCTCTTGCTCGTGTTTGTCGTTGTCTCAGAGGTGGAAATACTGCAGATGATGCTGACAGCGATAGTGATTTGGAAGTGGTTGCCGACTTCTTTCCTGTCAGCCTGCGTTGTCCTGTGAGCATTTGTCTACATATTTCTGTTTTCCCGCTAGTTATGAGATTGTCTTTATTATTTCGAATTTTAAGAAAGAATTCCATATATGAACAGTATCTATGTCATTGATCAATGAGCTGGTTGTCTCTCGATACTGGCCTTTTACATGCCACACTTTCCCTCAAGTTAAGAATGACATACATTCAAAAAAGTTAAGAATTACTGAGTTGACTCCAACTGAGTGGTTATCTAGCTGTATTTGGCTGCCATTATATTTAATATATTATCACAACGTACAAAATAAGCTTTTCCACAGTGCTTCACAGAATCCTGATATGCAAGCTTCAAGAGTTGAACATTTTTTTATCTGATAATATTTCACAGAATAGTGGATCCCGGATAAGGACCGCGGGAAGGTTCAAGCCTTGTGCTCACATGGGCTCTTTTGATCTGCAAACTTTTGTAGAGCTGAATCAACGGTCACGAAAGGTAAGATTTTATTCCTCCATATCCATAACTAGGATCTCTACATAATGAACATTGTACTTTGGTCCAACTTGTATTGGTCCATTTGGCAATGGACGGGCTAATTATGATTGGTTGGTACATTTAAATGCATACTTTCTTCAGATTGACGTGGCCCTGTTTTTAAAGACTTGTTGGTTTTTGCAGTGGCAATGTCCAACATGTCTGAAGAATTATTCTGTTGAGAGCTTGATCATTGATCGGTATTTCAACCGGATCGCTTCTCTGGTAGGTTTGAATAAGTCATATTCATTTTCTGTGTATTGTAAGCAGTTTTATGTCTTTCTTAGACTGTTCTTGTGATTCAGGTTCGGAATTGCAGTGAAGATGTCACTGAGATAGATGTGAAGCCTGATGGCTCTTGGCGTGTGAAGGGTGATGTTGCAGATATAAAATTGTCCCTGTGGCACCTGCCTGACGGCTCCCTGTGTGAACTGAAACAAGACACTAAACCTGTTGCTGGTGATGTAAAATCTGAAACTTCAAAAATTGGCAGCAGGGGAAATGTAGGCCTTAATGGATTGTGGGAAGCTAGTAAAGCTGTTGACATAAATCCCTCAAAGCCTATGAGCAGTAGCCACACTGGAATTTACAGGGATGGGGACTACCAAAGTGTGAGCGAGTGCAGCACGCAAATTGGTGAGATGTACAGAGTTGATGACAGACCACAGCAACAGCTTGAAGATGCAGACGTCATCGTTCTCAGTGACTCCGATGATGACAATGTTGTGACAGTGTCTCCACCAGCTGCCTATAGTGATGTTGGTGGTTTGGGATTTGCTCCCATTTCTGCGCCGGGAGTTGCTGAAAGTTACCAGGAGGGTGGTGTAGTTGGGGGCCTTGGCCTTGATTTGTTCAACGACAACAGCGATATTTTTGACATAACTTCCTGGTCTGCGCAACCCCAACCAGAGCAAGGGTTCAATTTTTTCGGAACTGATGTTCTACTTGGTTCTCACAATTCATCTGATGCAGCGCCAAGTGCTTATACCCTTGGCTGCCACGCTGGCTCTAGTGATACTTCCATGGTTCGAGATCCTTCTACCTGCCATGTACGCACCAGAAGCTTGGTCGATAACCTGTTGCCTTTTGGCAATGATGATTCTTCTCTGCGAATTTTTCTTCCTATTCAACCATCTGGCGTTCCCGTTCAGGAAGAACGGAATGGGCATGATAACATGTCAAATGGGGTCCAGCATGTTGATGACGATGAAGACGAAGATTGGATATCTCTTACACTTGCGGCGGGTGGAGGTAATAATGAACAGTCTGAGGCAGCAGATACGGTGAGCGCACAAGCACAAACCGCAGTGGAAGAGAGAAGGATGGAACCAGCAGACACGCTGAACCCACAAGCACAAATGACAGTGGAAGAGAGAAGGATGGAACCAGCAGATGTGTTGAACCCGCAAGCACAAATTGCAGTGGAAGAGACAAGGATAGAACCAGCAGATGCGTTGAACCCACAAGCACAAATTGCAGTGGAAGAGAGGACAGAACCAGGGGATACGTTGAGCCCACAAGAACAAGTTACAGTGGAAGAGAGAAGGACGGAACCAGCAGACATTGTTAACCCACAAGCGCAAACTGCAGTGGAAGAAAGAAGAATGGAACCGGCAGATACGGTGAACCGACAAGCACAGATTGCACTGGAAGAGAGAAGGATGGAACCATCTAGTGATGCTGGTTCGTCTCTTTAATATGAGTTCAGTTTTATCTTTCGTGTTATTTATTTATCCGTTTTTGTCCTGATTGATTAACTCTGCTGATAAACTGATTAAACTATGTGCCAGTGTTTAGTTGGTTTAGTGTTGACAGCCTTTTGTTTTTCTTTTCCTCGTGTTGTATGCAGAAGGCTCGCCTCCCAGCTTGAATGATGAGAGGCGTAACAAAGGGAATTCAAAAACAAGGGCTGAAAAGATATTTTCTCCTCCGCGGCAGCCTCGATCCGTCAGGCCTCGACTAACGACACAGCAGTGACGTTGAGTAGTTCTTCTTTTTTACTTTGGATAGCTGTCATGATGTACTTGTGGCCGATGTAAAGAAGAACCAACATGAATGTTTTCATAGTTTAACCTGATGTACAAACTACAGAAGTTTCAAGTGCTGGAAATGAGGGTGCGCCTGAAGGACATCTGACGGTGAGTGCTACAGCTCTCCTCTCCTAAGAATGTAAATTTGCAGTTTTGCGGAATAATGGGGGTATTTAGGTTTAAACTGGATTGTTTTAAGATAATACTCCCTCCGTTCCTAAATATAAGTCTTTATAGAAATTCCATAGTGGAATCTCTACATACGAAGCAAAATGAGTGAATGTACACTCTAAAATGCATCTATATACATCCGTATGTGGTCCATAGTGGAATCTCTACAAAGACTTATATTTAGGAACGGAGAGAGTACCATTCAGACTATATAGACAGCTACACCTTACGAAGGAAGAAAGGTGCACATGGCTCAGTTTTCAGATTGGTTTCTGCTTCTCTTTTACCTAGAAGATACAGATGGCTCTAGGCTTCCCAGCATCGATATGGAAGTATGCTATAATGCTATAGGTTAAGTGTCTTAGTCCATGGATGATATATCATATTGTATATCTTTCTTGTTTAAGCTTAAGCACAGTGAGCAGCATCATTCATGTATTCCTTTTTTTGTTTTGGTCTGAATGGAGATTCAGCTAGTGATGCATTCATATTGTTTACCAGATGAAAGTGTGATTGATCGAAAAAATAAATAAATCCACTGACGTGTTTGTGTTTTCAACATTATTGGTGCAGAGATAACAGGAAGTGAGCAAGGAAGGCTTGATAGGTTTTGTGGCAGTCTGCAGCATATTGTACATTTGACCACCAGACTATTATAAATATATATGTGCCGTGCCGAAATATGGAAGGCAAACAAATAGAGATGTGGGGGTTTGTGTATCGTTAAATTGTAATTAGGGGTTGGCTTGTGTGTTGCTAATTGAGTGAGATGCCCTAGTATACTTGGTACTCTTTCCGGAAATTAAATTATTTTTTCATGTTTATTATTATTATTATTACATTCTTTTCTTTTTTCATGTTTCTTGTGTATACTTGTTGCTCTTTCCTGAATTTTTTCCCATGATTATTGTTATTACTACATTCCATGAAGAAGAAATGCAGGTCAGGTGATGAACCTGGAAAATATAGCAT.

SEQ ID No.9 (TaMSIZ 1-3D genomic sequence, wheat (Triticum aestivum L.))):

CAAAACTGAAACCGATCGATCGATCGAACCGATTCACACGCCACCGCCGCCCCCTCGCCGCCGACCTTCACTGGGTCCAGAGCTCGCCGCCATGGGGGACCTGGCCTCCACCTGCAAGGTCGGTCCCCATCTGATCCTCCCCTCTCTTGCTTCTTTGCTTGCCTGCGATTCACTCTCCGGTTCCGGGGGTCGGTCGTCTAGCTAATCTAATCTAATCTAATCTAATCCTGAGTTGCTCTACTCCTACTTGTTCAGGACAAGCTTGCCTACTTCAGGATCAAGGAGCTCAAGGACGTGCTCATCCACCTCTCCCTCCCCAAGCACGGCAAGAAGCAGGTATCCATCTCCCCCCTCCCCATACTACTCTACGTATCATATATCCTTGTTGATGGATCCACTACTAGTCTAGTCGTACCCATCACAAGTCCATCCGCTATTACACATTCAATGGATGGATCCACTCATGTTTCCAAATAACTAGTCTAGCCTCTAGCCACCATGACAACAGCATCCTGTAAATTCATTCCTGCAAACCGCAAGTTCATTCAGCTTCTCATATGCATGCTGCACGAGGTCTTATCCAATCATCCCATGTATGTGTTGTTGTTGCAGGACCTCGTCGACAGGATCCTCGCCCTGCTCTCCGACGACCAAGGTCTGCCCACCTTCTTTCACCAACCCTCTCTGTGCCCCTGCAATGCACTTCTATCATCAACTTAAAACAATGCCACATTTTTTTTATCCTTGCAAAGCAGCCATAGCGTTTTTTTTTTTGGGGGAAGAAAAATCACCTGCTACTTCCTAACTAGCTCTGGTATGTATGTGCCTGCCTCAGCCCAATGGCACCTTGGCCGAGGGAGGAAGAATGCGCCCAGCAAGGAGGCGGTCGTCAAAATAGTCAACGACATATACAGGTTAGCTAGCTTCCATGCCATTTACCCTTCTTTCCTTCCTATTTCAGTGCTACTGGTACTTGGGTTATCACACCATTCATTCATTGCCATAGCAGGAAGATGCAAGTCCACGGTCCTCCTGACCTCGTGGTATCTCCTGCTCCTGCTCAGAACCAGCTGCCTGTCACGACTGATTTCAGCCGCATCATCAAAGCCAAAAAGGAACAATTGGGCCCCGACTCTGGCTGCCTTTGTGGTCAATCCTTTGTTCTCGGGAATGTGGTCAAGGTGTGCCCATCTGCCCTGCCCTGGCCTCCTCTGCCTGTCCTTCTCTCCAACATACAAAAAATGACCATGTTTTCTACACTTGTTTGATTATTTGTGTGCTTGCTTCTTCTAGTGCGACGACTGCCAAGTCCAGCAGCATATGGACTGCGTGCTCATTCCAGAGAAGCCTGCAGTGGGCGTGAGACCTGAAGCTCCAGAGCATTACTTTTGTCAGTTGTGCCGACTAATCCGAGCAGACCCGTATGCCCTCGTACAATCCACTTCTATTTGCTATTTCCTTCTGTGATTTGCCATCTTGACAAATATGATGCACCACTGTTACTGGAAACTCCGAAGCGATCAGACTATTTGTTGCTTCCAGCATTCAAAGTTAGCATGTCCAATCAAATCATGATCCTTATACATATACAACTCTACTGTTGTAACCTAGGTAGGGGTTTTAACGAACTTGTCAGGTCGAGAGTTGCATCAACTGTATGACTGATTGGTTGATTCAGACTCGTGTTATTTGCCAGTAGGCTGAAAAGGAACCTTCTCTCTAAAACTTTGTTCTAGTATATCATGACAATATCTGAGGGAACAAGATTTAAGCCAATAAGTTTCCTATTGATTCTATAGAACTTTGTCTCCATTCCTGCATGGATTGCTTGGAATTGAAATATTTTCTGCACATTTGCTACAGTGACTATTTTGAACCTGCAGATATTGGATTACTACTGGAAATCCTTTACTACCTGTGAGACTGATTACAAATGATGGGTGAGTGTAACTTTATGGGCAATGTATTCCTTGATAGTCTGGTATCTGATACACTATAATCATCACTTACTGAGCATTTCAAATTATATTGGAAGGAGTGACCTTCTTTGTAGTAGAATACTCATCGTTGGCTTTGCCTGTCACACTATTATGTGCTACATTCAGTCCACAGTAATCTTACAGAATTCTCAACCATTTGCAGAATGAATGTTCCTCAGAGTGTGGATAGAACTTTTCTACTTACTCGAGCTGAGAGGGAGACTGTTCAGAGAGTGGAATATGATATTCAGGTTTGATTCCCTTTTCCTCTGAGACTGATTACTGTCATCCTTTACATTTTCTTTCCACAAGGGCAATGACACTTTAATAATGTCATATGGAAATTATTAGCAGTGCGATGCTGCTTTGTCATAACCTTTTCTAAAGGGGAGGGTGCTATGAAATTAACTGAGTGGCATGTTCATGATTGCTTGTCATGTTGTCAAAGGCAGAACTCCTAAAAGAGTACCAAATTGCAATTCTTTTTTGTGATTTTACCTGGTGCTTTTCTCTTCATTATGGATAAATGCACTTTCTAACGTTATGTTTGTACAAGGACAAAATTCCTATGTCATATGGAAATTAAGGAGTGTTATATTCTCGTTATAGGGTTAAACTCCCTAGTTGGTTGTTTGTAAAGACAACACACTAGTAGTTTTTTTTGTGTATGTTATGCAGCATGTCGCTGATGAGGGCATGCTCCTTTGCAGGTTTGGTGTATGCTTCTGAACGACAAAGTTCAGTTCAGGATGCACTGGCCTCAGAATGCAGACTTACAAGTGAACGGTACAGATTTTGCTTTTCAGCTTTCTCGTACAGTTGAGATCGCATATATGATTCATGAGGTCCTATTTTTGTGTCCTCAGACTGAGTTTGAATCATTTTTATACTCCAAAATAGAGGTAGACATATAGTACCTTGAAAGAGGCCCTTGAGACATGTTGTAGTTCACTAGTATCAAGTAGTGAATTTATCAATTTATTTTTTGAAGCTTTTATTTGTTCATTTATAATTTTATATACTGTATGCAGATATGTACAGTGAGCCTTGTTTAGGTGTTGCAATGCATTTATCTTGGTAAAATCTTTTATGAGCAAGACATGGAGGATCATTCTTGATATTGGTTTCATGTTTTCATCAGGTATGCAAGTGCGAGTAGTTCCCAGGCCTAGCACTCAGTTACTAGGGATTAATGGACGGGATGATGGGCCGGTGGTGAGTGCACTCCTATTTCATCGTTAGTGCATCTTAACCAACAGAACTATGTCTATTATCTGTCTTTAGTCATCTTAAAGTTATCAGTTGCTTTTCTTGGCAATTCAATTTTTTTTGAGAAACAATTCAATTTAATTTAATTGTGAGTTCCCTTGTAAACAACATATCTGCACAGTTCAGAGCTTTTATGTTTTTGGACTGCTTTGCAGATAACAACCTTTTGCCGAGAAGGACAGAATAAAATAGTTTTATCAAGTGATGATGCCCGACCATTTTGTTTTGGGATCAGAATTGCAAAGAGGAGGACAGTTGACCAGGTTTTTTTTTCTCTCCTGTTACTTACCTAGCTGTTGAAGTGAATTGTAGAAGGTGCAAACTTTTCTGGCTTGCTGATGTTTGAGTTGCCTTTTCTGTGATGATTGGATTGCAATCTGAAAAACAATGATTTGTAAGTGGATCGTATATGTGCTCACAATAACTGAAACACTTAACATTGCCCCCCTAAATGTTCCTCAATAACAATTAATAAATCAGGTAACTTTGGTAAGCTCAATGGTCATTTCCCCCCTTTATGTTGATCAATTTTTCAACTTGTAGTTTCACAACCATATTTCTTTTTAACACATGTTTCGTTATGTTACTTCTAAACTTTGGATATTTTAAGTGATAATAAGCACGTTATCACCTATACTACAACATTACCTTTTCAGGTCCTAAACTTGGTGCCAAAGGAAGCTGATGGCGAGTCTTTTGAGGATTCTCTTGCTCGTGTTTGTCGCTGCCTCAGAGGTGGAAATACTACAGATGATGCTGACAGCGATAGTGATTTGGAAGTGGTTGCCGACTTCTTTCCTGTCAGCCTGCGTTGTCCTGTGAGCATTTGTCTACATATTTCCATTTTCATGTGTATGAGATGCCTTTTTGTTTCGAAATTTTAGAAAGAATACCATATATGAACAGTATCTATGTCGTTGATCAATGAGCTGGTTGTCTCTTGACACTGGCCTTTTGCATGCCACACTTTCCCTCTAGTTAAGAATGGCAGACCTTAAAAAAAGTTAAGATTACTGAGTTGACTCCAACCGAGTGGTTATCTAGCTGTATTTGGCTGCTATTTATTCATATATTATCACAACGTACAAACTAAGCTTTTCCACAGTGATTCACAGAATCCTGATATGCAAGTTTCAAGAGTTGATCATTTTTTTATCTGATAATATGTCACAGAATAGTGGATCCCGGATAAGGACCGCGGGAAGGTTCAAGCCTTGTGCTCATATGGGCTCTTTTGATCTGCAAACTTTTGTAGAGCTGAATCAACGGTCACGAAAGGTAAGATTTTATTCCTCCATATCCATAACTAGGATCCCTACATAATGAACATTGTACTTTGGTCCAACTTGTGTGGTCCATTTAGCAATGGACAGGGTAATTATGATTGGTTGGTCCATTTAAATGCATACTTTCTTCAGATTGACGTGGCCCTATTTTTAAAGACTTGTTGGTTTTTGCAGTGGCAATGTCCAACATGTCTGAAGAATTATTCTGTTGAGAGCTTGATCATTGATCGGTATTTCAACCGGATCGCTTCTCTGGTAGGTTTGAACAAGTCTTATTCATTTTCTGTGTATTGTTAGCAGTTTTATGTTTTTCTTAGACTGTTCCTGTGATTCAGGTTCGGAATTGCGGTGAAGATGTCACTGAGATAGATGTGAAGCCTGATGGCTCTTGGCGTGTGAAGGGTGATGTTGAAGATATAAAATTGTCCCTGTGGCACCTGCCTGACGGCTCCCTTTGTGAACTGAAACAAGACTCTAAACCTGTTGCTGGTGATGTAAAATCTGAAACTTCAAAAATTGGCAGCAGGGGAAATGTAGGCCTAAATGGATTGTGGGAAGCTAGTAAAGCTGTTGACATAAAGCCCTCAAAGCCTATGAGCAGTAGCCATACTGGAATTTACAGGGATGCGGACTACCTAAGTGTGAGCGAGTGCAGCACGCAAATTGGTGAGATGTACAGAGTTGATGACAGGCCACAGCAACAGCTCGAAGATGCAGATGTCATTGTTCTCAGTGACTCCGATGATGACAATGTTGTGACAGTGTCTCCACCAGCTGCCTATAGTGATGTTGGTGGTTTGGGATTTGCTCCCATTTCTGCGCCAGGAGTTGCTGAAAGTTACCAGGAGGGTGGTGTAGTTGGGGGCCTTGGCCTCGATTTGTTCAACGACAACAGCGATATTTTTGACATAACTTCCTGGTCTGCGCAACCCCAACCAGAGCAAGGGTTCAATTTTTTCGGAAATGATGTTCTACTTGGTTCTCACAATTCATCTGATGCAGCGCCAAGTGCTTATACCCTTGGCTGCCACGCTGGCTCTAGTGATACTTCCATGGTTCGAGATCCTTCTACCTGCCATGTACGCACCAGAAGCTTGGTCGATAACTTGTTGCCTTTCGGCAATGATGATTCCTCTCTGCGAATTTTTCTTCCTATTCAACCATCTGGCGTTCCCGTTCAGGAAGAACGGAATGGGCATGACAACATGTCAAATGGGGTCCAGCCTGTTGATGACGATGAAGACGAAGATTGGATATCTCTTACACTTGCGGCTGGTGGAGGTAGTAATGAACAGTCTGAGGCAGCAGATACGGTGAGCACACAAGCACAAATCGCAGTGGAAGAGAGAAGGATGGAACCAGCAGACACGCTGAACCCACAAGCACAAATGACGGTGGAAGAGAGAAGGATGGAACCAGCAGATGTGTTGAACCCGCAAGCGCAAATTGCAGTGGAAGAGACAATGATAGAACCAGCAGATGCGTTGAACCCACAAGCACAGATTGCAGTGGAAGAGAGGGCGGAACCAGCAGATACGTTGAGCCCACAAGCACAAGTTACAGTGGAAGAGAGAAGGATGGAACTAGCAGACATTGTTAACCCACAAGCGCAAATTGCAGTGGAAGAAAGAAGAATGGAACTGCCAGATACGGTGAACCGGCAAGCACAAGCACAGATTGCACTGGAAGAGAGAAGGATGGAACCATTGAGTGATGCTGGTTCGTCTCTTTAATATGAGTTCAGTTTTATCTTTTGTGTTATTTATCTGTTTTTCTCCTGATTGATTAACTCTTCTGATAAACTGATTAAACTGGCCCAATATTTTGTGCCAGTGTTTAGTTCGTTTAGTGTGTGTTGACAGCCTTTGTTTTTCTTTTCGTCGTGTTGTTTGCAGAAGGCTCGCCTCCCAGCTTGAATGATGACAGGCGCAACAAAGGGAATTCGAAAACAAGGGCTGAAAAGATATTTTCTCCTCCGCGGCAGCCTCGATCCGTCAGGCCTCGACTAACGACACAGCAGTGACGTTGAGTAGTTCTTCTTTCTTACTTTGGATAGCTGTCATGATGTACTTGTGGCCCATGTAAAGAAGAACCAACATGAATGTTTTCATAGTTTAACCTGATGTACAAACTACAGAAGTTTCAAGTGCTGGAAATGAGGGTGTGCCTGAAGGACATCTGACGGTGAGTACAGCTCTCCTCTCCTAAGGATGCAAATTTGCAGTTTTGGGGAATAACGGGGATATTTAGGTTCAAATTGGATTGTTTTTAGATAAATATACCATGCAGACCATATAGATAGCTACTTGTTGAAGAAGGAAGAAATGTGCACATGACTCATTTTTCGGTTTAGTTTTGCTTCTCTTCTACCTAGAAGATACAGATGGCTCTAGTCTTCCCAACATCGATATGGAAGTATGCTATAATGCTATATAGGTTAACTGTCTTAGTCCATGGATGATATATCATATTGTGGTTTCGTTGATTCACATGCATTATTTCCTCTGTCCTAAACTGATTTCGGTTTATCAAGCTGGGAGATTCCAAAATAGGCATCTTATTTCAGTGATGGTGAAAACTGGGAAGATTTATCCAATATTTGTAGTAGTATATCTTTGTCGTTTAAGCTTGGCACAGTGAGTAGCATCATTCATGTAGTCCTTGTTTCTTTTCGTCGCATGGATATTCAGCTAGTGATGCATTCAAATTGTTTACCAGGTGATGAAGTGCAATTGATCGGAAAAGAAAAAAAAAATCGACTGACGTGTTTGTGTTTTCAACATTGGTGCAGACATAACAGGAAGTGAGCAAGGAAGGCTTGATAGGTTTTGTGGCAGTCTGCAGCATATTGTACATTTGACCACCAGACTTATATAAATATATATGTGCCGTGCCGAAATGGAAGAAGGCAAACAAATAGAGATGTGGAGGTTTGTGTATCATTAAATTGTAATTAGGGGTTGGCTTGTGTGTTGCTAATTGAGATGCCCTAGTATACTTGGTACTCTTTCCGGAAATGAAATCCCTTATTTTTTCATGTTTGGAATTTTTTTCATGATTATTATTATTATTACATTCAATTCCATGAAGG.

The present application is described in detail above. It will be apparent to those skilled in the art that the present application can be practiced in a wide range of equivalent parameters, concentrations, and conditions without departing from the spirit and scope of the application and without undue experimentation. While the application has been described with respect to specific embodiments, it will be appreciated that the application may be further modified. In general, this application is intended to cover any variations, uses, or adaptations of the application following, in general, the principles of the application and including such departures from the present disclosure as come within known or customary practice within the art to which the application pertains. The application of some of the basic features may be done in accordance with the scope of the claims that follow.

Claims (9)

1. Any of the following uses of a protein or a substance that modulates the content or activity of said protein:

d1 Regulating drought resistance of the plant;

D2 Preparing a product for regulating and controlling drought resistance of plants;

d3 Cultivating drought resistance-altering plants;

D4 Preparing and cultivating drought resistance-changing plant products;

the protein is TaSIZ A protein, taSIZ A protein, taSIZ A protein and/or TaSIZ A protein:

the TaSIZ A protein is A1), A2) or A3) as follows:

a1 A protein having an amino acid sequence of SEQ ID No. 2;

A2 A protein which has more than 75 percent of identity with A1) and has the same function through the substitution and/or deletion and/or addition of one or more amino acid residues of the amino acid sequence shown in SEQ ID No.2 in the sequence table;

A3 Fusion proteins obtained by ligating a tag to the N-terminal or/and the C-terminal of A1) or A2);

the TaSIZ1-3B protein is H1), H2) or H3) as follows:

h1 A protein having an amino acid sequence of SEQ ID No. 5;

h2 A protein which has more than 75 percent of identity with H1) and has the same function through the substitution and/or deletion and/or addition of one or more amino acid residues of the amino acid sequence shown in SEQ ID No.5 in the sequence table;

h3 Fusion proteins obtained by ligating a tag to the N-terminal or/and the C-terminal of H1) or H2);

The TaSIZ1-3D protein is I1), I2) or I3) as follows:

I1 A protein having an amino acid sequence of SEQ ID No. 8;

I2 A protein which has more than 75 percent of identity with I1) and has the same function through the substitution and/or deletion and/or addition of one or more amino acid residues of the amino acid sequence shown in SEQ ID No.8 in the sequence table;

I3 Fusion proteins obtained by ligating a tag to the N-terminal or/and the C-terminal of I1) or I2).

2. The method according to claim 1, wherein the substance is any one of the following B1) to B9):

B1 A nucleic acid molecule encoding said protein;

B2 An expression cassette comprising the nucleic acid molecule of B1);

b3 A recombinant vector comprising the nucleic acid molecule of B1) or a recombinant vector comprising the expression cassette of B2);

B4 A recombinant microorganism comprising the nucleic acid molecule of B1), or a recombinant microorganism comprising the expression cassette of B2), or a recombinant microorganism comprising the recombinant vector of B3);

B5 A transgenic plant cell line comprising the nucleic acid molecule of B1) or a transgenic plant cell line comprising the expression cassette of B2);

B6 A transgenic plant tissue comprising the nucleic acid molecule of B1) or a transgenic plant tissue comprising the expression cassette of B2);

B7 A transgenic plant organ comprising the nucleic acid molecule of B1) or a transgenic plant organ comprising the expression cassette of B2);

b8 A nucleic acid molecule that reduces the protein content or activity;

b9 An expression cassette, a recombinant vector, a recombinant microorganism, a transgenic plant cell line, a transgenic plant tissue or a transgenic plant organ comprising the nucleic acid molecule of B8).

3. The method according to claim 2, wherein the nucleic acid molecule encoding the TaSIZ A protein is a DNA molecule having the sequence of SEQ ID No.1 at positions 217 to 3006;

The nucleic acid molecule encoding the TaSIZ A-3B protein is a DNA molecule with the encoding sequence being 240 th to 3017 th positions of SEQ ID No. 4;

the nucleic acid molecule encoding the TaSIZ-3D protein is a DNA molecule whose coding sequence is positions 92-2902 of SEQ ID No. 7.

4. The method according to any one of claims 1 to 3, wherein the substance for controlling the protein content or activity is a substance for increasing the protein content or activity, the drought resistance of the plant is increased, and the drought resistance-altering plant is a drought resistance-increasing plant;

The substance for regulating and controlling the protein content or the activity is a substance for reducing the protein content or the activity, the drought resistance of the plant is reduced, and the drought resistance changing plant is cultivated to reduce the drought resistance.

5. The method according to claim 1 to 4, wherein the plant is M1) or M2) or M3) or M4):

m1) monocotyledonous plants;

M2) a gramineous plant;

m3) wheat plants;

M4) wheat.

6. The method comprises the following steps:

x1) a method for improving drought resistance of a plant, comprising expressing the protein of claim 1 in the plant or increasing the content or activity of the protein of claim 1 in the plant to improve drought resistance of the plant;

X2) a method for cultivating a plant with improved drought resistance, comprising expressing the protein of claim 1 in the plant or increasing the content or activity of the protein of claim 1 in the plant to obtain a plant with improved drought resistance;

X3) a method for reducing drought resistance in a plant comprising reducing the amount or activity of a protein according to claim 1 in the plant, or knocking out a gene encoding a protein according to claim 1 in the plant, or reducing the expression level of a gene encoding a protein according to claim 1 in the plant, thereby reducing drought resistance in the plant;

X4) a method for breeding drought-resistance-reduced plants, comprising reducing the content or activity of the protein of claim 1 in plants, or knocking out the gene encoding the protein of claim 1 in plants, or reducing the expression level of the gene encoding the protein of claim 1 in plants, to obtain a plant of interest having reduced drought resistance.

7. The method according to claim 6, wherein the methods X1) and X2) are carried out by introducing a gene encoding the protein according to claim 1 into plants.

8. The method according to claim 6 or 7, wherein the plant is M1) or M2) or M3) or M4):

m1) monocotyledonous plants;

M2) a gramineous plant;

m3) wheat plants;

M4) wheat.

9. The protein of claim 1 or the agent that modulates the protein content or activity of any one of claims 1-3.