CN120436638A - Manufacturing process of wearable oxygen free radical real-time dynamic monitor - Google Patents
Manufacturing process of wearable oxygen free radical real-time dynamic monitorInfo
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- CN120436638A CN120436638A CN202510951977.XA CN202510951977A CN120436638A CN 120436638 A CN120436638 A CN 120436638A CN 202510951977 A CN202510951977 A CN 202510951977A CN 120436638 A CN120436638 A CN 120436638A
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Abstract
The invention relates to the technical field of oxygen radical detection, in particular to a wearable oxygen radical monitor and a manufacturing process thereof, and is characterized by comprising a shell, a monitoring probe, a detection device and a detection device, wherein the monitoring probe comprises a basal layer, a first electrode, a second electrode, a first reagent layer and a second reagent layer, the first reagent layer is made of lauric acid modified superoxide dismutase, the second reagent layer is made of perfluorosulfonic acid polymer, an interface part of the first electrode is connected with a circuit module, an interface part of the second electrode is connected with the circuit module, a pilot needle is detachably connected with a connecting hole, the pilot needle comprises a holding part and a needle body connected with the holding part, and a groove is formed in the lower part of the needle body. The structure improvement enables the prepared monitor to dynamically monitor the oxygen radical change condition of the human body in real time under the condition of not taking blood.
Description
Technical Field
The invention relates to the technical field of oxygen radical detection, in particular to a manufacturing process of a wearable oxygen radical monitor.
Background
Harmful oxygen or free radicals called active oxygen are generated as by-products in the oxygen metabolism process in the human body, and the oxygen free radicals have various adverse effects on the human body, for example, lipid peroxides are generated in the human tissue, and lipid peroxidation is a physiological effect continuously occurring in cell membranes, which causes oxidative damage of unsaturated lipids.
In the prior art, malondialdehyde is one of measurement methods capable of determining lipid peroxidation degree, the method is mainly implemented by using an HLPC method and a TBARS method, the HLPC method is implemented by using expensive equipment, the TBARS method is required to carry out complex pretreatment on a sample, in the detection method, blood collection is required for each detection, the detection efficiency is low, and the detection accuracy is easily affected in the process of transferring the blood collection to an instrument.
Disclosure of Invention
The invention aims to solve the technical problem of providing a wearable oxygen free radical real-time dynamic monitor and a manufacturing process thereof, so that the manufactured monitor can dynamically monitor the oxygen free radical change condition of a human body in real time under the condition of not taking blood.
In order to solve the technical problems, the invention adopts the following technical scheme:
a wearable oxygen radical monitor comprising:
The skin-care device comprises a shell, wherein a circuit module is arranged in the shell, a through connecting hole is formed in the shell, and a pasting material layer used for pasting with skin is arranged at the lower part of the shell;
The monitoring probe comprises a basal layer, a first electrode, a second electrode, a first reagent layer and a second reagent layer, wherein the basal layer comprises a strip-shaped needle implantation part and an interface part connected with one end of the needle implantation part, the width of the interface part is larger than that of the needle implantation part, the first electrode is connected with the first side of the basal layer, the first electrode covers part of the needle implantation part and the interface part, the second electrode is connected with the second side of the basal layer, the second electrode covers part of the needle implantation part and the interface part, the first reagent layer is arranged on the first side of the needle implantation part of the first electrode, the second reagent layer is arranged on the second side of the needle implantation part of the second electrode, the first reagent layer is made of lauric acid modified superoxide dismutase, the interface part of the second reagent layer is made of perfluorosulfonic acid polymer, the interface part of the first electrode is connected with the circuit module, and the interface part of the second electrode is connected with the circuit module.
The pilot needle, pilot needle detachably connects in the connecting hole, and the pilot needle includes the portion of gripping and connects in the needle body of portion of gripping, needle body lower part is equipped with the recess, and under the first state, the monitoring probe is connected in the recess, and under the second state, the pilot needle inserts skin tissue in coordination with the planting needle portion of monitoring probe, and the pilot needle is pulled out from connection Kong Fanxiang, makes the planting needle portion of monitoring probe detain in skin tissue.
In the wearable oxygen radical monitor structure, the substrate layer is made of polyethylene terephthalate.
In the wearable oxygen radical monitor structure, the first electrode is made of gold.
In the wearable oxygen radical monitor structure, the second electrode is silver.
Further, in the structure of the wearable oxygen radical monitor, the wearable oxygen radical monitor further comprises a protective sleeve, and the protective sleeve is coated on the needle body of the pilot needle in the first state.
Further, in the structure of the wearable oxygen radical monitor, the circuit module comprises a main power supply circuit module, a switch circuit module, a power supply circuit module, a main control circuit module, a voltage signal processing module and a wireless communication module;
The main power supply circuit module is electrically connected with the power supply circuit module through the switch circuit module;
The power circuit module is respectively and electrically connected with the first electrode, the second electrode, the voltage signal processing module, the main control circuit module and the wireless communication module and is used for respectively supplying power to the first electrode, the second electrode, the voltage signal processing module, the main control circuit module and the wireless communication module in a variable-voltage mode;
The voltage signal processing module comprises a voltage transmission unit, a voltage comparison unit and a reference signal unit, wherein the voltage transmission unit is electrically connected with the first electrode and the second electrode and is used for transmitting sensing voltage, and the voltage comparison unit is used for comparing the sensing voltage with the reference voltage pre-stored by the reference signal unit to obtain a voltage difference signal;
the main control circuit module is respectively and electrically connected with the switch circuit module, the voltage signal processing module and the wireless communication module, and is used for carrying out analog-to-digital conversion on the voltage difference signal and transmitting the digital signal to the wireless communication module;
the wireless communication module is used for sending digital signals to the terminal.
Further, in the structure of the wearable oxygen radical monitor, the wireless communication module is a bluetooth module.
Further, in the wearable oxygen radical monitor structure, the substrate layer is provided with a through hole, a third electrode is arranged on a first side of the through hole of the substrate layer, and the third electrode is connected with the second electrode through the through hole.
The invention also relates to a manufacturing process of the wearable oxygen radical monitor, which comprises the following steps:
Manufacturing a shell and a circuit module;
manufacturing a monitoring probe, manufacturing a substrate layer, respectively arranging a first electrode on a first side of the substrate layer, arranging a second electrode on a second side of the substrate layer, connecting a first reagent layer to the first side of the first electrode through an adhesive, and connecting a second reagent layer to the second side of the second electrode through the adhesive;
the monitoring probe is connected into the needle body groove of the pilot needle, the pilot needle is connected to the shell, the interface part of the monitoring probe is fixed to the shell, and the power circuit module and the voltage transmission unit in the circuit module are respectively and electrically connected with the first electrode and the second electrode.
The invention has the advantages that 1, lauric acid modified superoxide dismutase (LA-SOD) is adopted as an enzyme detection reagent for the first time, and is acid-resistant, alkali-resistant and high-temperature-resistant, 2, long-term stability, lauric acid modified superoxide dismutase is acid-resistant, alkali-resistant, high-temperature-resistant and long in storage period, 3, wearing type dynamic monitoring of oxygen free radical change, real-time clinical monitoring, 4, portable, small and exquisite and light detector, and 5, rapid and instant dynamic monitoring.
Drawings
FIG. 1 is an exploded view of a wearable oxygen radical monitor structure according to an embodiment of the present invention;
fig. 2 is an enlarged view of a portion a of fig. 1;
FIG. 3 is an exploded view of a monitoring probe of a wearable oxygen radical monitor according to an embodiment of the present invention;
FIG. 4 is a schematic plan view of a monitoring probe of a wearable oxygen radical monitor according to an embodiment of the present invention;
FIG. 5 is a schematic diagram of a circuit module of a wearable oxygen radical monitor according to an embodiment of the present invention;
description of the reference numerals:
1. 11, connecting holes;
2. Monitoring probes, 21, a basal layer, 22, a first electrode, 23, a third electrode, 24, a second electrode, 26, a first reagent layer, 27, a second reagent layer, 281, a needle implantation part, 282 and an interface part;
3. A pilot needle, 31, a holding part, 32, a needle body, 321 and a groove;
4. and a circuit module.
Detailed Description
In order to describe the technical contents, the achieved objects and effects of the present invention in detail, the following description will be made with reference to the embodiments in conjunction with the accompanying drawings.
Referring to fig. 1 to 5, a wearable oxygen radical monitor according to an embodiment of the present invention includes:
The skin-care device comprises a shell 1, wherein a circuit module 4 is arranged in the shell 1, a through connecting hole 11 is formed in the shell 1, and an adhesive material layer for adhering to skin is arranged at the lower part of the shell 1;
The monitoring probe 2 comprises a basal layer 21, a first electrode 22, a second electrode 24, a first reagent layer 26 and a second reagent layer 27, wherein the basal layer 21 comprises an elongated needle-planting portion 281 and an interface portion 282 connected to one end of the needle-planting portion 281, the width of the interface portion 282 is larger than that of the needle-planting portion 281, the first electrode 22 is connected to the first side of the basal layer 21, the first electrode 22 covers part of the needle-planting portion 281 and the interface portion 282, the second electrode 24 is connected to the second side of the basal layer 21, the second electrode 24 covers part of the needle-planting portion 281 and the interface portion 282, the first reagent layer 26 is arranged on the first side of the needle-planting portion 281 of the first electrode 22, the second reagent layer 27 is arranged on the second side of the needle-planting portion 281 of the second electrode 24, the first reagent layer 26 is made of lauric acid modified superoxide dismutase, the second reagent layer 27 is made of perfluorosulfonic acid polymer, the interface portion 282 of the first electrode 22 is connected to the circuit module 4, and the interface portion 282 of the second electrode 24 is connected to the circuit module 282;
The pilot needle 3, the pilot needle 3 detachably connects in connecting hole 11, and the pilot needle 3 includes the portion of gripping 31 and connects in the needle body 32 of portion of gripping 31, the needle body 32 lower part is equipped with recess 321, and in the first state, monitor probe 2 is connected in recess 321, and in the second state, the pilot needle 3 inserts skin tissue in coordination with the planting needle portion 281 of monitor probe 2, and the pilot needle 3 is pulled out in the opposite direction from connecting hole 11, makes the planting needle portion 281 of monitor probe 2 detain in skin tissue.
In the above embodiment, referring to fig. 1, the connecting hole 11 is a stepped counter bore, and the holding portion 31 of the pilot needle 3 is provided with a stepped mortise-tenon structure matched with the counter bore, so that the pilot needle 3 can only be pulled out from one side facing away from the needle body 32.
In the above embodiment, the method for using the wearable oxygen radical monitor is as follows, the product is that before use, the pilot needle 3 and the monitoring probe 2 are connected in the connection relationship of the first state, and the pilot needle 3 is connected to the connection hole 11 of the housing 1 to form a whole, when in use, the lower part of the housing 1 is pressed to be attached to the skin surface, so that the pilot needle 3 cooperates with the needle implantation portion 281 of the monitoring probe 2 to be inserted into the skin tissue, in order to ensure continuous and stable wearing, the medical adhesive tape can be attached to the housing 1 for fixing the housing 1 on the skin, to avoid falling off, then the pilot needle 3 is pulled out reversely, the needle implantation portion 281 of the monitoring probe 2 is retained in the skin tissue, namely in the second state, at this time, the first electrode 22 and the second electrode 24 can generate voltage change according to the electrochemical action of the oxygen radicals, and the signal of the voltage change can be acquired in real time through the circuit module 4, thereby achieving the purpose of monitoring the oxygen radicals in real time.
The working principle of oxygen radical detection through the first reagent layer and the second reagent layer is as follows:
The compound molecule generates homolytic cleavage of covalent bond under the action of enzymatic electron transfer, oxidation-reduction reaction or photo-thermal action, and forms atoms or groups with unpaired electrons, which become free radicals. Superoxide dismutase (SOD) has the capability of scavenging oxygen free radicals (O 2 -), and can catalyze the disproportionation reaction of the oxygen free radicals (O 2 -), so as to generate oxygen and hydrogen peroxide. The hydrogen peroxide generates electrochemical reaction under the action of the working electrode, and electron transfer occurs, so that a current signal is generated. The current is proportional to the concentration of oxygen radicals, and the concentration of oxygen radicals can be obtained by detecting the current. The specific reaction equation is as follows:
Specifically, the material of the first reagent layer 26 is lauric acid modified superoxide dismutase (LA-SOD) matched with a certain amount of adhesive, the activity of the lauric acid modified superoxide dismutase is more than or equal to 40000U/mg, the purity is more than or equal to 98%, the material of the second reagent layer 27 is Nafion film (perfluor sulfonic acid polymer), the film can repel negatively charged interferents (such as ascorbic acid and uric acid), the signals collected by the electrodes are ensured to be generated by disproportionation reaction, and the effect of the layer is to limit the permeation of the interferents (such as ascorbic acid and uric acid) and improve the specificity.
Specifically, since the probe needs to be inserted into subcutaneous tissue of the human body, in order to reduce discomfort after the needle implantation portion 281 is inserted into the human body, and at the same time, to ensure sufficient strength, the width of the needle implantation portion 281 is 0.3mm, and in order to ensure that the probe can contact with tissue fluid of the human body after being inserted into the skin, the length of the probe needle implantation portion 281 is 7mm. The electrons generated after the electrochemical reaction need to be transferred to the circuit module 4 through the first electrode 22 and the second electrode 24, and in order to secure the stability of the connection of the printed circuit board, the width of the interface portion 282 is 2mm and the length is 6mm.
In a preferred embodiment, the base layer 21 is made of polyethylene terephthalate. The material has good mechanical property, chemical stability and dimensional stability, can provide stable support for the electrode layer and the reagent layer, is soft and has small foreign body sensation when inserted into human tissues.
In a preferred embodiment, the first electrode 22 is gold. Attached to the base layer 21 by an electroplating process to a thickness of 0.05mm. Compared with other materials, the gold has strong physical and chemical stability, and can not be dissolved at the joint of the gold and human tissue fluid for a long time, thereby causing harm to human body.
In a preferred embodiment, the second electrode 24 is silver. This electrode is a reference electrode of the sensor, and is attached to the bottom of the base layer 21 by electroplating using metallic silver (Ag) to a thickness of 0.05mm. The two electrodes are respectively arranged on the upper and lower surfaces of the basal layer 21, so that the cross-sectional area of the part of the sensor implanted into the skin can be reduced, and the wound area can be reduced.
In a preferred embodiment, the needle body 32 of the pilot needle 3 is covered with a protective sheath in the first state.
As a preferred embodiment, the circuit module 4 includes a main power supply circuit module 4, a switch circuit module 4, a power supply circuit module 4, a main control circuit module 4, a voltage signal processing module, and a wireless communication module;
The main power supply circuit module 4 is electrically connected with the power supply circuit module 4 through the switch circuit module 4, and the main power supply circuit module 4 is used for supplying power for the control battery to the circuit board, when the pilot needle 3 is pulled out, the battery is connected, and the first electrode 22 and the second electrode 24 are in a working state.
The power circuit module 4 is respectively and electrically connected with the first electrode 22, the second electrode 24, the voltage signal processing module, the main control circuit module 4 and the wireless communication module, and is used for respectively supplying voltage to the first electrode 22, the second electrode 24, the voltage signal processing module, the main control circuit module 4 and the wireless communication module, and the power circuit also comprises a voltage reduction module, a current limiting module, a voltage stabilizing module and the like, so that stable and satisfactory voltage can be conveniently provided for each module unit.
The voltage signal processing module comprises a voltage transmission unit, a voltage comparison unit and a reference signal unit, wherein the voltage transmission unit is electrically connected with the first electrode 22 and the second electrode 24 and is used for transmitting sensing voltage, and the voltage comparison unit is used for comparing the sensing voltage with the reference voltage pre-stored by the reference signal unit to obtain a voltage difference signal;
The main control circuit module 4 is respectively and electrically connected with the switch circuit module 4, the voltage signal processing module and the wireless communication module, and the main control circuit module 4 is used for carrying out analog-to-digital conversion on the voltage difference signal and transmitting the digital signal to the wireless communication module;
For example, the main control circuit module 4 controls the switch circuit module 4 to supply power to the power circuit module 4 every 5 minutes according to the sampling period, at this time, the monitoring probe 2 generates electrochemical reaction, and the generated signal is amplified by the voltage transmission unit, filtered and processed by the voltage comparison unit to obtain a signal, and the signal is transmitted to the main control circuit module 4;
the wireless communication module is used for sending digital signals to the terminal.
In a preferred embodiment, the wireless communication module is a bluetooth module.
In the above embodiment, taking the terminal as the mobile phone as an example, after the instrument is worn, the battery of the main power supply circuit module 4 supplies power to the circuit module 4, at the moment, the APP is opened by the mobile phone, bluetooth is adopted to pair with the instrument, so that the current signal acquired by the instrument can be transmitted to the mobile phone, corresponding operation is carried out by the mobile phone, and finally the current signal is displayed by the APP. For example, the bluetooth module realizes connection with the mobile phone, and transmits data to the mobile phone according to a time period of 5 minutes, and when the bluetooth module is disconnected with the mobile phone, the bluetooth module can inform the main control circuit module 4 of temporarily storing data, and the bluetooth module integrally packages and transmits the data when the connection is restored next time.
As a preferred embodiment, the substrate layer 21 is provided with a through hole, and the first side of the substrate layer 21 at the through hole is provided with a third electrode 23, and the third electrode 23 is connected to the second electrode 24 through the through hole.
In the above embodiment, the second electrode 24 is connected to the third electrode 23 through a through hole, so that the reference electrode is located on the same surface of the substrate layer 21, and is conveniently connected to the circuit board, so as to realize data analysis.
The mobile phone port can display the concentration of oxygen free radicals in real time after receiving the data, and performs data statistics and storage to establish an oxygen free radical concentration curve graph.
The invention also relates to a manufacturing process of the wearable oxygen radical monitor, which comprises the following steps:
Manufacturing a shell 1 and a circuit module 4;
Manufacturing the monitoring probe 2, manufacturing the substrate layer 21, respectively arranging the first electrode 22 on the first side of the substrate layer 21, arranging the second electrode 24 on the second side of the substrate layer 21, connecting the first reagent layer 26 to the first side of the first electrode 22 through an adhesive, and connecting the second reagent layer 27 to the second side of the second electrode 24 through an adhesive;
The monitoring probe 2 is connected to the pilot needle 3 in the needle body 32 groove 321, the pilot needle 3 is connected to the housing 1, the interface 282 of the monitoring probe 2 is fixed to the housing 1, and the power circuit module 4 and the voltage transmission unit in the circuit module 4 are electrically connected to the first electrode 22 and the second electrode 24, respectively.
The application scene of the wearable oxygen radical monitor related to the invention can comprise:
1. Scientific research
Mitochondrial function research and antioxidant drug screening.
2. Clinical application
Ischemia reperfusion injury monitoring, inflammatory response monitoring, oxidative stress research and monitoring. Through interdisciplinary collaboration (material science, microelectronics, biomedical engineering), the oxygen radical monitor can realize dynamic, high-sensitivity detection of active oxygen species, and provides a key tool for oxidative stress research.
3. Industrial process
Evaluation of oxidative stability of food.
The foregoing description is only illustrative of the present invention and is not intended to limit the scope of the invention, and all equivalent changes made by the specification and drawings of the present invention, or direct or indirect application in the relevant art, are included in the scope of the present invention.
Claims (9)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202510951977.XA CN120436638A (en) | 2025-07-10 | 2025-07-10 | Manufacturing process of wearable oxygen free radical real-time dynamic monitor |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202510951977.XA CN120436638A (en) | 2025-07-10 | 2025-07-10 | Manufacturing process of wearable oxygen free radical real-time dynamic monitor |
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| CN120436638A true CN120436638A (en) | 2025-08-08 |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1754097A (en) * | 2003-02-24 | 2006-03-29 | 汤浅真 | Active oxygen species measuring device |
| CN115844397A (en) * | 2022-12-20 | 2023-03-28 | 深圳市优维健康科技有限公司 | Dynamic blood glucose meter |
| CN116897015A (en) * | 2020-12-23 | 2023-10-17 | 雅培糖尿病护理公司 | Analyte sensor and method with reduced interferent signal |
-
2025
- 2025-07-10 CN CN202510951977.XA patent/CN120436638A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1754097A (en) * | 2003-02-24 | 2006-03-29 | 汤浅真 | Active oxygen species measuring device |
| CN116897015A (en) * | 2020-12-23 | 2023-10-17 | 雅培糖尿病护理公司 | Analyte sensor and method with reduced interferent signal |
| CN115844397A (en) * | 2022-12-20 | 2023-03-28 | 深圳市优维健康科技有限公司 | Dynamic blood glucose meter |
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