CN1289115C - A kind of traditional Chinese medicine for improving immunity and preparation method thereof - Google Patents

Abstract

The invention relates to a traditional Chinese medicine for improving immunity and a preparation method thereof. The method is characterized in that American ginseng is crushed into coarse powder, ethanol is used for percolation after ethanol soaking, and ethanol is recovered to obtain extract; soaking Atractylodis rhizoma and Polygoni Multiflori radix in water, percolating with ethanol, and recovering ethanol under reduced pressure to obtain extract; decocting Poria and fructus Lycii in water, mixing decoctions, concentrating under reduced pressure, precipitating with ethanol, removing supernatant, and collecting precipitate to obtain extract; the three extracts are combined and dried, and are pulverized into fine powder, so that the active component of the medicine is prepared.

Description

A kind of traditional Chinese medicine for improving immunity and preparation method thereof

technical field

The invention relates to a pure traditional Chinese medicine preparation which improves body immunity and has anti-fatigue effect, and further relates to a preparation method of the traditional Chinese medicine preparation.

Background technique

At present, there are a variety of traditional Chinese medicine preparations that can improve immunity on the market. The status of existing similar products is shown in the table below.

Existing traditional Chinese medicine preparations for improving immunity name prescription composition dosage form effect Application range Shi Quan Da Bu Wan Codonopsis, Atractylodes macrocephala, Poria cocos, licorice, angelica, Chuanxiong, white peony root, rehmannia glutinosa, astragalus, cinnamon pill warming blood For qi and blood deficiency, pale complexion, shortness of breath, palpitation, dizziness and spontaneous sweating, fatigue and weakness, and limbs are not warm Guipi Wan Codonopsis pilosula, Atractylodes macrocephala, Astragalus licorice, Poria cocos, polygala, jujube seed, longan meat, angelica, woody fragrance, jujube pill Replenishing Qi and invigorating the spleen, nourishing blood and calming the nerves Heart and spleen deficiency, shortness of breath and palpitations, insomnia and dreaminess, dizziness, dizziness, fatigue, loss of appetite, Buzhong Yiqi Pill Astragalus, Codonopsis, Licorice, Atractylodes macrocephala, Angelica, Cimicifuga, Bupleurum, Chenpi pill Tonify the middle and replenish qi, raise the yang and lift the sink Weakness of the spleen and stomach, depression of central qi, fatigue, lack of appetite, abdominal distension, prolonged diarrhea Tian Qi powder (cooked) Tian Qi pink Invigorate Qi and blood, strengthen body Weakness, excessive fatigue Brain Relax Capsules Rhodiola, Schisandra, Ginkgo biloba capsule Anti-fatigue, anti-hypoxia, improve memory Adolescents with fatigue and poor memory and middle-aged and elderly people with memory loss Deep Sea Dragon Capsule Sea dragon, seahorse, deer antler, sheep whip, cnidium, epimedium, cistanche, schisandra, ginseng, astragalus, etc. capsule Warming and tonifying kidney yang, tonifying marrow and essence Soreness of the waist and knees, aversion to cold, fatigue, dizziness, tinnitus, palpitations and insomnia caused by deficiency of kidney yang Erxian Paste Ginseng, medlar, antler glue, tortoise shell glue, bullwhip, astragalus, rehmannia glutinosa, Polygonum multiflorum, Schisandra chinensis, etc. ointment Nourishes yin and yang, nourishes qi and nourishes blood Treatment of qi and blood deficiency, mental fatigue and fatigue Shenling Baizhu Capsules Ginseng, Poria cocos, Atractylodes macrocephala, Chinese yam, white flat, lotus seed, coix seed, amomum, bellflower, licorice Capsules invigorate the spleen and replenish qi Fatigue, lack of appetite and loose stools Ginseng and Shouwu Capsules Red Ginseng, Radix Polygoni Multiflori Capsules Nourish liver and kidney, replenish qi and blood Weakness of qi and blood, premature graying of beard and hair, neurasthenia, forgetfulness, insomnia, loss of appetite, excessive fatigue

Some of the existing immunity-enhancing products have incomplete formulas and cannot effectively regulate the body. Some medicines have too much flavor and are inconvenient to prepare. Existing similar products are all made by traditional Chinese medicine preparation methods, the preparation technology is backward, the removal rate of invalid impurities is low, the content of active ingredients is low, the extractum has strong hygroscopicity, and the stability of the preparation is poor.

Contents of the invention

The object of the present invention is to provide a kind of pure traditional Chinese medicine preparation which improves body immunity and has anti-fatigue effect. Compared with the compatibility of existing similar prescriptions, the preparation is more targeted and has better clinical curative effect.

Medicine of the present invention is mainly made up of following crude drug (parts by weight):

1-5 parts of American ginseng, 2-12 parts of Polygonum multiflorum, 2-12 parts of Atractylodes macrocephala, 2-12 parts of Poria cocos, 2-12 parts of medlar.

The preparation method of the Chinese medicine preparation providing immunity of the present invention comprises the following steps:

(1) Take 1 to 5 parts of American ginseng and grind it into the coarsest powder, add 40% to 80% ethanol to soak for more than 72 hours, then percolate with ethanol, and recover ethanol from the percolation liquid under reduced pressure to obtain the extract (1) for later use;

(2) Take another 2-12 parts of Atractylodes Rhizome and 2-12 parts of Polygonum multiflorum, add 5 to 10 times the weight of medicinal materials to soak in water, wash with water until the color is very light, then use ethanol for percolation, and the percolation liquid is decompressed to recover ethanol, extract

(2) standby;

(3) Take 2 to 12 parts of Poria cocos and 2 to 12 parts of medlar, add 5 to 10 times the weight of the medicinal material and decoct twice for 1 to 2 hours each time, combine the decoction, concentrate under reduced pressure, and use 60 % to 90% ethanol for three times, discard the supernatant, and take the precipitate to obtain the extract (3);

(4) above-mentioned three kinds of extracts are combined and dried, and powdered into fine powder, the active component of medicine of the present invention has just been made. The active components of the medicine of the present invention can be prepared into any medicine or health product in oral dosage form, such as capsules, tablets, granules, oral liquid and the like.

The present invention has the following advantages:

1. In the prescription of the present invention, American ginseng, Polygonum multiflorum, Poria cocos, Lycium barbarum, and Atractylodes macrocephala five-flavored Chinese medicine are all commonly used tonic Chinese medicines. Among them, American ginseng tonifies qi and nourishes yin, clears heat and promotes body fluid, clears heat and stops bleeding; Polygonum multiflorum nourishes liver, nourishes kidney, nourishes blood, and dispels wind; Poria cocos calms the heart and calms the nerves; wolfberry nourishes kidney and essence, nourishes the liver and improves eyesight, nourishes blood and calms the nerves; Stomach, dampness, and neutralization. The prescription combines warm and cool, without cold and heat bias, enters the meridian and takes into account the five internal organs, comprehensively regulates, and is targeted for multiple organ dysfunction, physiological function disorder, and decreased body vitality caused by qi and blood damage and yin and fluid consumption. Regulatory effect.

2. Compared with other similar products, the traditional Chinese medicine preparation of the present invention has less medicinal taste but more refined taste, and has more targeted health care and therapeutic effects on improving body immunity and anti-fatigue.

3. The method for extracting and purifying the traditional Chinese medicine formula of the present invention is more scientific, and the prepared dosage form is advanced and modern, convenient to take, and has good clinical curative effect.

Description of drawings

Fig. 1 is the preparation process flow chart of the traditional Chinese medicine preparation for improving immunity of the present invention.

Detailed ways

Example 1 Preparation example

Daily Prescription Compatibility

Prescription: 2 grams of American ginseng, 3 grams of Polygonum multiflorum, 10 grams of Atractylodes macrocephala, 3 grams of Poria cocos, 2 grams of medlar

Preparation method: take American ginseng and crush it into the coarsest powder, add 40% ethanol to soak for 72 hours, then percolate with ethanol, recover ethanol from the percolation solution under reduced pressure, and obtain extract (1) for later use.

Separately take Atractylodes Rhizoma Atractylodes Rhizome and Polygonum Polygoni Multiflori, add 5 times the weight of medicinal materials to soak in water, wash with water until the color is very light, then use ethanol to percolate, and depressurize the percolation liquid to recover ethanol to obtain extract (2) for later use.

Then take the two flavors of Poria cocos and medlar, add 5 times the weight of the medicinal materials and decoct them twice, 1 hour each time, combine the decoction liquid, concentrate under reduced pressure, and precipitate with 60% ethanol for three times, discard the supernatant, and take the precipitate Wude extract (3). The above three kinds of extracts are combined and dried, powdered into fine powder, and supplementary materials are added to prepare oral preparations.

Embodiment 2 Preparation example

Daily Prescription Compatibility

Prescription: 3 grams of American ginseng, 6 grams of Polygonum multiflorum, 2 grams of Atractylodes macrocephala, 5 grams of Poria cocos, 10 grams of medlar

Preparation method: take American ginseng and crush it into the coarsest powder, add 60% ethanol to soak for 72 hours, then percolate with ethanol, and recover ethanol from the percolation solution under reduced pressure to obtain extract (1) for later use.

Separately take Atractylodes Rhizoma Atractylodes Rhizome and Polygonum Polygoni Multiflori, add 8 times the amount of medicinal materials to soak in water, wash with water until the color is very light, then use ethanol for percolation, and the percolation solution is decompressed to recover ethanol to obtain the extract (2) for later use.

Then take the two flavors of Poria cocos and medlar, add 8 times the amount of medicinal materials to decoct twice, 2 hours each time, combine the decoction liquid, concentrate under reduced pressure, and precipitate with 60% to 80% ethanol three times, discard the supernatant, Take the precipitate to obtain the extract (3). The above-mentioned extracts are combined and dried, powdered into fine powder, and a little auxiliary material is added to make granules.

Embodiment 3 Preparation example

Daily Prescription Compatibility

Prescription: 5 grams of American ginseng, 11 grams of Polygonum multiflorum, 5 grams of Atractylodes macrocephala, 2 grams of Poria cocos, 4 grams of medlar

Preparation method: take American ginseng and crush it into the coarsest powder, add 70% ethanol to soak for 4 days, then percolate with ethanol, and recover ethanol from the percolation solution under reduced pressure to obtain extract (1) for later use.

Separately take Atractylodes Rhizoma Atractylodes Rhizome and Polygonum Polygoni Multiflori, add 8 times the amount of medicinal materials to soak in water, wash with water until the color is very light, then use ethanol for percolation, and the percolation solution is decompressed to recover ethanol to obtain the extract (2) for later use.

Then take the two flavors of Poria cocos and medlar, add 8 times the amount of medicinal materials to decoct twice, combine the decoction liquid, concentrate under reduced pressure, and precipitate with 60% to 80% ethanol three times, discard the supernatant, and take the precipitate ointment (3). The above-mentioned extracts are combined and dried, powdered into fine powder, and a little auxiliary material is added to make capsules.

Embodiment 4 Preparation example

Daily Prescription Compatibility

Prescription: 2 grams of American ginseng, 4 grams of Polygonum multiflorum, 5 grams of Atractylodes macrocephala, 12 grams of Poria cocos, 4 grams of medlar

Preparation method: take American ginseng and crush it into the coarsest powder, add 80% ethanol to soak for 72 hours, then percolate with ethanol, and recover ethanol from the percolation solution under reduced pressure to obtain extract (1) for later use.

Separately take Atractylodes Rhizoma Atractylodes Rhizoma Atractylodes Rhizome and Polygonum Polygoni Multiflori, add 10 times the amount of medicinal materials to soak in water, wash with water until the color is very light, then use ethanol to percolate, and depressurize the ethanol to recover ethanol to obtain the extract (2) for later use.

Then take Poria cocos and Lycium barbarum two flavors, add 10 times the amount of medicinal materials to decoct twice, combine the decoction liquid, concentrate under reduced pressure, precipitate three times with 90% ethanol, discard the supernatant, and take the precipitate to obtain the extract ( 3). The above three extracts are combined and dried, powdered into fine powder, added a little auxiliary material, and pressed into tablets.

Example 5 pilot test process

Get the medicinal material of 500 times embodiment 1 daily prescription quantity

For the above five flavors, American ginseng is crushed into the coarsest powder, soaked in 60% ethanol for 72 hours, then percolated with ethanol, and the ethanol is recovered from the percolated solution under reduced pressure to obtain the extract (1) for later use. Separately take Atractylodes Rhizoma Atractylodes Rhizome and Polygonum Polygoni Multiflori, add 8 times the amount of medicinal materials to soak in water, wash with water until the color is very light, then use ethanol for percolation, and the percolation solution is decompressed to recover ethanol to obtain the extract (2) for later use. Then take the two flavors of Poria cocos and medlar, add 8 times the amount of medicinal materials to decoct twice, combine the decoction liquid, concentrate under reduced pressure, and precipitate with 60% to 80% ethanol three times, discard the supernatant, and take the precipitate ointment (3). The above-mentioned extracts are combined and dried, powdered into fine powder, added appropriate amount of auxiliary materials, mixed evenly, and made into oral preparations (granules, capsules, tablets, etc.).

Produce 3 batches according to above preparation process, the result is as follows: batch number Feeding amount (kg) Amount of extract (kg) Total saponin content% Crude polysaccharide content (g) 20021121 65 3.25 10.6 2.2 20021122 65 3.32 9.8 2.0 20021123 65 3.21 11.2 2.3

According to the above preparation process, the pilot test is carried out, the yield of the extract is about 5%, the total saponin content (calculated as ginsenoside Re) is not less than 8%, and the crude polysaccharide (calculated as dextran) is not less than 2%.

Embodiment 6-9 is the pharmacological research test embodiment of medicine of the present invention

Sample: the fine powder prepared in Example 5 of the present invention.

Experimental animals: 220 second-class Kunming male mice, weighing 18-22 g, purchased from the Experimental Animal Center of China Institute for the Control of Pharmaceutical and Biological Products, certificate number: Yidongzi No. 017.

Sample dose; the doses of the test substance given to mice are: medium dose 0.13g/kg.bw, high dose 0.40g/kg.bw, low dose 0.03g/kg.bw, high, medium and low dose They are respectively equivalent to 30 times, 10 times and 2 times the recommended daily intake per kilogram of body weight.

Sample dose preparation method: take by weighing an appropriate amount of medicine fine powder of the present invention, prepare the sample solution of the high-dose group with 0.25% sodium carboxymethylcellulose (CMC) solution, the sample solution of the middle-dose group and the low-dose group is then obtained by the high-dose group The sample solution was prepared by diluting with 0.25% CMC solution in proportion.

The mice in each experimental group were given the sample solution of each dose group of this product by oral gavage continuously, and the mice in the normal control group were given 0.25% CMC solution.

Instruments and reagents:

Instruments: YSI 1500 Lactic Acid Analyzer (USA), Hitachi 7060 Automatic Biochemical Analyzer (Japan), Fisher M-300DR Electronic Balance (Germany), TP-1000 Animal Balance (Xiangyi Balance Instrument Factory), TGL-16G Centrifuge (Shanghai Anting Scientific Instrument Factory), LD5-2A centrifuge (Beijing Medical Centrifuge Factory), 721 spectrophotometer (Shanghai Third Analytical Instrument Factory), timer, 120L swimming bucket, lead skin, 20μl quantitative blood collection tube, 0.5ml and 1.5ml centrifuge tubes.

Reagents: hemolytic agent for lactic acid determination, enzyme membrane, 5mmol/L lactic acid standard solution, buffer solution (YSI Company of the United States), urea nitrogen determination kit (Beijing Zhongsheng Biological Engineering Technology Company), 5% trichloroacetic acid (TCA), Glucose standard solution (100mg/dL), 72% sulfuric acid, anthrone reagent (containing 0.05% anthrone and 1% thiourea, prepared with 72% sulfuric acid).

experiment method:

Screening: After the mice entered the laboratory for 2 days, they were subjected to a swimming screening test with a lead skin bearing 4% of their body weight, and the survivors were taken out after 10 minutes.

Animal grouping: 60 mice screened by swimming were dried, weighed and randomly grouped according to body weight, and entered into the weight-bearing swimming test; 120 unscreened mice were randomly grouped according to body weight, and biochemical assay tests were performed. Each experiment was divided into 4 groups, namely normal control group, low dose group, middle dose group and high dose group. The way and time of giving the test substance: give each dose of the experimental group mice continuous oral gavage of the corresponding sample solution for 28 days, gavage once a day, each mouse gavage amount of 0.5ml, and weigh every week. Adjust the concentration of the test substance.

Embodiment 6 mouse weight-bearing swimming test

The mice were continuously orally gavaged with the test substance for 28 days, and weighed. After 0.5 hours of gavage for the last time, a lead skin with 5% body weight was placed on the base of the tail, and swimming was carried out in a swimming bucket with a water depth of 30 cm and a temperature of 25 degrees. Record the swimming time of the mice from entering the water to exhaustion and death, as the swimming time of the mice.

The effect of drugs on the swimming endurance of mice (using SigmaStat statistical program to carry out analysis of variance to compare the differences between variables, if the homogeneity of variance or normality cannot meet the requirements of analysis of variance, then perform after data conversion).

Table 1 shows the effect of the drug fine powder of the present invention on the body weight of the mice in the endurance group. Compared with the normal control group, there was no significant difference in the actual body weight before swimming of mice in each dose group, p>0.05.

Table 1. The effect of drug fine powder on the body weight of endurance group mice group Dose (g/kg.bw) Group weight (g) ^{1, 2} Weight before swimming (g) ^{1, 3} Normal control group, low dose group, middle dose group, high dose group -0.030.130.40 18.6±2.0(15)18.8±1.9(15)18.8±2.0(15)18.6±1.9(15) 34.3±28.7(15)37.2±2.6(15)37.7±2.2(15)37.0±2.6(15)

¹ X±SD The number of animals in brackets

² analysis of variance: F=0.044, P=0.99

³ Analysis of variance: F=0.58, P=0.63

Table 2 shows the effect of the drug fine powder of the present invention on the swimming endurance of weight-bearing mice. Compared with the normal control group, the swimming time of the mice in the high dose group was significantly prolonged, p<0.05.

Table 2. The effect of drug fine powder on the swimming time of mice group Dose (g/kg.bw) Number of animals (only) Swimming time (min) ^{1, 2} Normal control group, low dose group, middle dose group, high dose group -0.030.130.40 15151515 34.3±28.756.6±40.465.5±43.673.3±37.3 ^*

¹ X±SD; ² Analysis of variance: F=3.00, P=0.038; ^* Compared with the control group, P<0.05

Example 7 Blood lactic acid content (mmol/L)

Give the mouse continuous oral gavage of the test substance for 28 days, weigh it, and 0.5 hours after the last gavage, use a 20 μl quantitative blood collection tube to collect blood from the orbit, and transfer the collected blood sample quantitatively to a mixed solution containing 40 μl enzymolysis inhibitor (hemolytic agent). ) in a centrifuge tube, mix well, and the second is the blood lactic acid of the mouse in a quiet state. Then place the mouse in a swimming bucket with a water depth of 30 cm and a temperature of 30 degrees to swim for 10 minutes, take out the mouse, and immediately collect blood from the orbit with a 20 μl quantitative blood collection tube, and quantitatively transfer the collected blood sample to a mixed solution containing 40 μl of enzyme hydrolysis inhibitor (hemolytic agent). ) in a centrifuge tube, and mix well, this is the blood lactic acid of the mouse after exercise; the mouse swims or recovers for 20 minutes, and then collects blood from the orbit with a 20 μl quantitative blood collection tube, and quantitatively transfers the collected blood sample to a mixture containing 40 μl of enzyme hydrolysis inhibitor In the centrifuge tube of solution (hemolytic agent), mix well, this is the blood lactic acid of mouse recovery period after swimming.

Table 3 shows the effect of the fine powder of the drug of the present invention on the body weight of mice in the lactic acid group. Compared with the normal control group, there was no significant difference in the actual body weight before swimming of mice in each dose group, p>0.05.

Table 3. Effects of drugs on body weight of mice in lactic acid group group Dose (g/kg.bw) Group weight (g) ^{1, 2} Weight before swimming (g) ^{1, 3} Normal control group, low dose group, middle dose group, high dose group -0.030.130.40 19.9±1.8(15)19.8±1.6(15)20.0±1.2(15)19.7±1.4(15) 39.7±2.9(15)38.6±2.6(15)39.0±2.4(15)39.4±3.2(15)

^1X ±SD

² Analysis of variance: F=0.060, P=0.98

³ Analysis of variance: F=0.040, P=0.75

Table 4. Effects of drugs on blood lactate levels in mice. Compared with the normal control group, there was no significant difference in the blood lactic acid levels of the mice in the swimming dose experimental groups, p>0.05; immediately after swimming, the blood lactic acid levels of the mice in the high-dose group were significantly reduced, p<0.05; 20 minutes after swimming , there was no significant difference in blood lactic acid levels of mice in each dose group, p>0.05.

Table 4. Effects of Drugs on Blood Lactate Levels in Mice group Dose (g/kg.bw) Blood lactic acid content before swimming (mmol/L) ^1,2 Blood lactic acid content after swimming (mmol/L) ^1,3 Blood lactic acid content after 20 minutes (mmol/L) ^{1, 4} Normal control group, low dose group, middle dose group, high dose group -0.030.130.40 2.03±0.562.03±0.351.90±0.461.92±0.42 5.60±1.7845.26±1.574.72±0.723.95±0.69 ^* 3.05±1.212.60±0.622.78±0.552.30±0.50

^1X ±SD

² ANOVA (mean root transformation): F=0.35, P=0.79

³ Analysis of variance: F=4.13, P=0.011, ^* Compared with the normal control group, p<0.05

⁴ analysis of variance (self-log transformation): F=1.84, P=0.15

Table 5 shows the effects of the drug fine powder of the present invention on the growth and elimination of blood lactic acid in mice. Compared with the normal control group, after swimming, the increase of blood lactic acid in mice in the high-dose group was significantly reduced, p<0.05; 20 minutes after swimming, there was no significant difference in the elimination of blood lactic acid in mice in each dose group, p>0.05.

Table 5. Effects of Drugs on Increase and Elimination of Blood Lactate in Mice group Dose (g/kg.bw) Blood lactic acid content before swimming (mmol/L) ^1,2 Blood lactic acid content after swimming (mmol/L) ^1,3 Normal control group, low dose group, middle dose group, high dose group -0.030.130.40 3.57±1.873.22±1.5428.2±0.792.03±0.88 ^* 2.55±1.332.66±1.581.94±0.781.65±0.84

^1X ±SD

² Analysis of variance: F=3.25, P=0.029, ^* Compared with the normal control group, p<0.05

³ ANOVA (square root transformation): F=1.64, P=0.19

Embodiment 8 blood urea nitrogen content (mmol/L)

The mice were continuously orally gavaged with the test substance for 28 days, weighed, and 0.5 hours after the last gavage, the mice were placed in a swimming bucket with a water depth of 30 cm and a temperature of 30 degrees for swimming for 90 minutes, and the mice were taken out. After 1 hour, the eyeballs were picked for blood collection, and the serum was prepared by centrifugation. Determination of serum urea nitrogen levels.

Table 6 shows the effect of the drug fine powder of the present invention on the body weight of mice in the urea nitrogen group. Compared with the normal control group, there was no significant difference in grouping and body weight before swimming, p>0.05.

Table 6. The influence of medicine fine powder of the present invention on urea nitrogen body weight group Dose (g/kg.bw) Group weight (g) ^{1, 2} Weight before swimming (g) ^{1, 3} Normal control group, low dose group, middle dose group, high dose group -0.030.130.40 19.8±1.4(15)20.0±1.5(15)19.9±1.7(15)19.5±1.4(15) 40.5±2.4(15)39.6±2.6(15)40.2±3.9(15)38.5±2.6(15)

^1X ±SD

² ANOVA: F=0.29, P=0.83

³ Analysis of variance (square root transformation): F=1.40, P=0.25

Table 7 shows the effect of the drug fine powder of the present invention on the serum urea nitrogen content of mice after swimming. Compared with the normal control group, after swimming, the blood urea nitrogen of mice in each dose group decreased significantly, p<0.05.

Table 7. Effect of fine powder of medicine of the present invention on mouse serum urea nitrogen content group Dose (g/kg.bw) Number of animals (only) Serum urea nitrogen content (mmol/L) ^1,2 Normal control group, low dose group, middle dose group, high dose group -0.030.130.40 14141414 10.09±1.008.12±0.98 ^* 8.08±0.68 ^* 8.38±1.80 ^*

^1X ±SD

² Analysis of variance: F=9.73, P<0.0001, ^* Compared with normal control group, p<0.05

Embodiment 9 liver glycogen content (mg/00g)-anthrone method

The above-mentioned mice whose blood lactic acid was measured continued to be gavaged for 3 days, and the last gavage was performed for 0.5 hours.

Table 8 shows the effect of the fine powder of the drug of the present invention on the glycogen content of mice. Compared with the normal control group, the glycogen content of mice in the high dose group increased significantly, p<0.05.

Table 8 The influence of drug fine powder of the present invention on mouse glycogen content group Dose (g/kg.bw) Number of animals (only) Liver glycogen content (mg/100g) ^1,2 Normal control group, low dose group, middle dose group, high dose group -0.030.130.40 14141414 2284.4±1052.72376.9±877.52581.1±1171.64294.8±676.6 ^*

^1X ±SD

² Analysis of variance: F=12.7, P<0.0001, ^* Compared with normal control group, p<0.05

The experimental results show that: after 28 days of continuous oral gavage of the fine powder of the present invention to mice, compared with the normal control group, after swimming, the swimming time of the high-dose group mice was significantly prolonged (p＜0.05); , the level of lactic acid in the high-dose group mice was significantly reduced (p<0.05), and the increase in blood lactic acid in the mice of the dose group was significantly less (p<0.05) (before swimming, the blood lactic acid levels of the mice in each group had no significant difference); after swimming, the serum urea nitrogen levels of the mice in each dose group were significantly reduced (p<0.05); the liver glycogen content of the mice in the high dose group was significantly increased, p<0.05. According to the judging criteria in "Functional Evaluation Procedures and Testing Methods of Health Foods", the hot powder of the drug of the present invention has an anti-fatigue effect on mice.

Claims (3)

1. Chinese medicine that improves immunity, make the raw materials of effective components medicine and consist of by weight:

1～5 part of Radix Panacis Quinquefolii, 2～12 parts of Radix Polygoni Multiflori, 2～12 parts of the Rhizoma Atractylodis Macrocephalaes, 2～12 parts in Poria, 2～12 parts of Fructus Lycii.

2. the Chinese medicine of the described raising immunity of claim 1 is characterized in that, this medicine is a peroral dosage form.

3. the preparation method of Chinese medicine of the described raising immunity of claim 1 comprises the steps:

(1) get Radix Panacis Quinquefolii and be ground into coarse powder for 1～5 part, add 40%～80% soak with ethanol more than 72 hours, the reuse ethanol percolation, the percolate decompression recycling ethanol, extractum (1) is standby;

(2) get 2～12 parts of 2～12 parts of the Rhizoma Atractylodis Macrocephalaes and Radix Polygoni Multiflori in addition, add the water logging bubble of 5～10 times of medical material weight, wash with water near colourless after, use ethanol percolation instead, the percolate decompression recycling ethanol, extractum (2) is standby;

(3) get 2～12 parts of 2～12 parts in Poria and Fructus Lycii again, the decocting that adds 5～10 times of medical material weight boils secondary, and each 1-2 hour, merge decoction liquor, concentrating under reduced pressure, the ethanol precipitate with ethanol with 60%～90% three times is abandoned supernatant, and taking precipitate gets extractum (3);

(4) above-mentioned three kinds of extractum merge drying, are ground into fine powder, have just made the active component of medicine of the present invention.