CN113980810A

CN113980810A - A cell culture dish unit with liquid supply and discharge pipelines

Info

Publication number: CN113980810A
Application number: CN202111406570.7A
Authority: CN
Inventors: 宋志兵; 张锴; 吴定; 张舒乐; 冯振雄
Original assignee: Hunan Kaiqi Shidai Biotechnology Co ltd
Current assignee: Hunan Kaiqi Shidai Biotechnology Co ltd
Priority date: 2021-11-24
Filing date: 2021-11-24
Publication date: 2022-01-28

Abstract

The invention relates to a cell culture dish unit with a liquid supply and drainage pipeline, which comprises: the device comprises a feeding unit, a collecting unit, a cell culture dish, a main pipeline, a first pipe pressure valve and a peristaltic pump; the first pipe pressure valve and the peristaltic pump are arranged on the main pipeline at intervals; the feeding unit is connected with the main pipeline, and the connection position of the feeding unit and the main pipeline is positioned at the upstream of the first pipe pressure valve; the collecting unit is connected with the main pipeline, and the connecting position of the collecting unit and the main pipeline is positioned between the first pipe pressure valve and the peristaltic pump; the end part of the main pipeline, which is positioned at the downstream of the peristaltic pump, is integrally arranged with an end cover of the cell culture dish, and the end surface of the end part does not exceed the inner side surface of the end cover. The invention has excellent integral sealing performance and is beneficial to ensuring the stability and cleanness of the inside of the whole culture dish.

Description

Cell culture dish unit with liquid supply and drainage pipeline

Technical Field

The invention relates to a cell culture dish unit with a liquid supply and discharge pipeline.

Background

Cell culture is a method for researching animal cell behaviors, which is established in the beginning of the twentieth century, and is characterized in that cells are taken out from tissues in vivo, the in vivo growth environment is simulated, the cells are grown and propagated under the conditions of sterility, proper temperature, pH value and nutrition, and the structure and the functions of the cells are maintained. The cell preparation refers to a process that scientific researchers culture a high-purity target cell final product by using a relatively mature cell culture scheme, and the high-purity target cell final product can be directly applied to the scientific research and clinical fields after function and quality detection.

The operations of feeding, draining and the like in the cell preparation process are very important, the feeding and draining operations are usually realized in a manual operation mode in the cell preparation process at present, and the cell culture dish is usually required to be opened in the operation process, so that the environment in the culture dish is easily polluted. Moreover, the culture dish for different cells is affected by the shape of the culture dish, so that the manual operation is difficult, and the problems of incomplete liquid updating and the like often occur; for example, the culture dish, which is generally used for adherent cell culture, is mainly flat in shape and laterally placed, and has a lot of inconvenience for manual operation.

Disclosure of Invention

The invention aims to provide a cell culture dish unit with a liquid supply and discharge pipeline.

To achieve the above object, the present invention provides a cell culture dish unit having a liquid supply and discharge line, comprising: the device comprises a feeding unit, a collecting unit, a cell culture dish, a main pipeline, a first pipe pressure valve and a peristaltic pump;

the first pipe pressure valve and the peristaltic pump are arranged on the main pipeline at intervals;

the feeding unit is connected with the main pipeline, and the connection position of the feeding unit and the main pipeline is positioned at the upstream of the first pipe pressure valve;

the collecting unit is connected with the main pipeline, and the connecting position of the collecting unit and the main pipeline is positioned between the first pipe pressure valve and the peristaltic pump;

the end part of the main pipeline, which is positioned at the downstream of the peristaltic pump, is integrally arranged with an end cover of the cell culture dish, and the end surface of the end part does not exceed the inner side surface of the end cover.

According to one aspect of the invention, the feed unit comprises: the device comprises a plurality of first containers for storing nutrient solution, a plurality of second containers for storing enzyme, a first branch pipe connected with the first containers, a second branch pipe connected with the second containers, and branch pipe pressure valves respectively arranged on the first branch pipe and the second branch pipe;

the first and second legs are in a spaced, staggered arrangement on the main conduit.

According to an aspect of the invention, the collecting unit comprises: the collecting device comprises a plurality of collecting containers, collecting branch pipes connected with the collecting containers, collecting pipes connected with the main pipeline, and collecting pipe pressure valves respectively arranged on the collecting branch pipes;

one end of the collecting branch pipe is connected with the collecting container, and the other end of the collecting branch pipe is connected with the collecting pipe;

the collection container comprises a collection container cover and a collection container body detachably connected with the collection container cover;

the end of the collecting branch pipe is integrally arranged with the collecting container cover.

According to an aspect of the invention, the cell culture dish further comprises: a bottle body detachably connected with the end cap;

the bottle body includes: a body portion and a neck portion provided at one side of the body portion;

the bottom part of one end of the bottle body part, which is connected with the bottle neck part, is an inclined plane, and the rest part is a plane;

support legs are arranged at the edges of the inclined surfaces;

the bottom ends of the supporting legs are flush with the plane of the bottom of the bottle body part;

the end cover or the bottle body is provided with a medical breathable filter membrane for ventilating and blocking liquid.

According to an aspect of the invention, further comprising: a culture dish bearing table;

the culture dish plummer includes: the device comprises a base, a lifting seat, a driving device and a detection device for detecting the internal state of a culture dish arranged on the lifting seat;

the driving device is supported on the base;

one end of the lifting seat is a rotating connecting end which is rotatably connected with the base, and the other end of the lifting seat is a lifting connecting end which is connected with the driving device;

under the driving action of the driving device, the lifting connecting end can do lifting motion relative to the base;

the bottle body of the cell culture dish is detachably arranged on the lifting seat.

According to one aspect of the invention, the base comprises: the device comprises a frame body and a bottom support arranged on the lower side of the frame body;

the lifting seat is positioned on the inner side of the frame body and above the bottom support;

and rotating shafts which are used for being rotationally connected with the frame body are respectively arranged on two opposite sides of the rotating connecting end of the lifting seat.

According to one aspect of the invention, the lifting seat is a frame structure which is provided with a mounting position for mounting the cell culture dish;

the installation position includes: a first hollow portion and a second hollow portion communicating with each other;

the second hollow part is provided with an opening at the end part of the rotating connecting end of the lifting seat;

the bottle body of the cell culture dish is positioned in the first hollow part, and the bottle neck part of the cell culture dish is positioned in the second hollow part;

in the width direction of the lifting seat, the lifting seat is provided with light-transmitting channels which penetrate through the lifting seat on two opposite sides of the mounting position;

the light-transmitting channel is arranged at the position of the first hollow part close to the lifting connecting end of the lifting seat.

According to one aspect of the invention, the bottom of the lifting seat is provided with a support structure;

the support structure includes: a first support structure and a second support structure;

the first support structure is arranged along the inner side edge of the bottom of the first hollow part;

the second support structure is connected to portions of the inside edges of the second hollow portion on opposite sides of the bottom thereof.

According to one aspect of the present invention, the first support structure is an elongated structure, which is continuously or intermittently disposed at an inner side edge of a bottom of the first hollow portion;

the second supporting structure is a plate-shaped body, and two opposite ends of the second supporting structure are fixedly connected with the inner side edges of the parts at two opposite sides of the bottom of the second hollow part respectively;

the second support structure is spaced from the end opening of the rotating link end.

According to an aspect of the present invention, the driving device includes: the driver is used for outputting linear displacement, and the fork arm is connected with the driver;

the yoke comprises: a driver connecting arm and a lifting seat connecting arm;

the connecting arm of the lifting seat is perpendicular to the connecting arm of the driver;

a linear channel is arranged on the bottom support;

one end of the lifting seat connecting arm, which is far away from the driver connecting arm, penetrates through the linear channel to be hinged with the lifting connecting end of the lifting seat;

the lifting seat connecting arm is positioned at the lower side of the bottom support, and one side, which is far away from the driver connecting arm, is hinged with the movable end of the driver;

a rubber damping structure is arranged at the hinged position of the driver connecting arm and the driver and is used for silent rotation of the hinged position;

the driver connecting arm is embedded with a position sensor for detecting the moving position of the driver connecting arm and a limiting structure for limiting the maximum displacement of the driver connecting arm.

According to one embodiment of the invention, the whole sealing performance is excellent, and the invention is beneficial to ensuring the stable and clean inside of the whole culture dish.

According to one scheme of the invention, the cell culture dish realizes the closed construction of the cell culture dish unit by combining the structures of the feeding unit, the collecting unit, the cell culture dish, the pipeline and the like, so that the whole cell culture process can be effectively realized as long as the cleanliness of the whole culture system is ensured, no extra manual work is needed, and the risk of the pollution of the culture dish caused by the manual work is effectively avoided. In addition, the structure of the invention also effectively realizes the automation in the cell culture process, and further effectively improves the culture efficiency.

According to one scheme of the invention, the cover body and the pipeline of the cell culture dish are directly and integrally arranged, so that the problem that the sealing property between the pipeline and the cover body is difficult to solve during system assembly is effectively solved, and the risk that the joint position of the pipeline and the cover body is easily corroded by pollutants is also effectively avoided. In addition, the structure that the pipeline and the cover body are integrally arranged further facilitates the connection with the bottle body of the culture dish, effectively eliminates the contact to the inside of the culture dish in the connection process, greatly ensures the cleanliness of the whole system and is beneficial to ensuring the normal operation of the whole period of the cell culture process.

Drawings

FIG. 1 is a block diagram schematically showing a cell culture dish unit according to an embodiment of the present invention;

FIG. 2 is a view schematically showing the structure of a cell culture dish according to an embodiment of the present invention;

FIG. 3 is a diagram schematically illustrating the connection of a culture dish support platform to a cell culture dish according to one embodiment of the present invention;

FIG. 4 is a block diagram schematically illustrating a culture dish carrier platform according to one embodiment of the invention;

FIG. 5 is a bottom view schematically illustrating a culture dish carrier platform according to one embodiment of the invention;

FIG. 6 is a block diagram schematically illustrating a base according to an embodiment of the present invention;

FIG. 7 is a block diagram schematically illustrating a lift seat according to one embodiment of the present invention;

FIG. 8 is a block diagram schematically illustrating a yoke according to an embodiment of the present invention;

FIG. 9 is a side view schematically showing a culture dish carrier platform according to an embodiment of the invention.

Detailed Description

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the embodiments will be briefly described below. It is obvious that the drawings in the following description are only some embodiments of the invention, and that for a person skilled in the art, other drawings can be derived from them without inventive effort.

In describing embodiments of the present invention, the terms "longitudinal," "lateral," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in an orientation or positional relationship that is based on the orientation or positional relationship shown in the associated drawings, which is for convenience and simplicity of description only, and does not indicate or imply that the referenced device or element must have a particular orientation, be constructed and operated in a particular orientation, and thus, the above-described terms should not be construed as limiting the present invention.

The present invention is described in detail below with reference to the drawings and the specific embodiments, which are not repeated herein, but the embodiments of the present invention are not limited to the following embodiments.

As shown in FIG. 1, according to one embodiment of the present invention, a cell culture dish unit having a liquid supply and discharge line of the present invention comprises: the device comprises a feeding unit 1, a collecting unit 2, a cell culture dish 3, a main pipeline 4, a first pipe pressure valve 5 and a peristaltic pump 6. In the present embodiment, the first pipe pressure valve 5 and the peristaltic pump 6 are provided on the main line 4 at intervals. The feeding unit 1 is connected with the main pipeline 4, and the connection position of the feeding unit 1 and the main pipeline 4 is positioned at the upstream of the first pipe pressure valve 5; the collecting unit 2 is connected with the main pipeline 4, the position where the collecting unit 2 is connected with the main pipeline 4 is located at the downstream of the first pipe pressure valve 5, and the connecting position of the collecting unit 2 and the main pipeline 4 is located between the first pipe pressure valve 5 and the peristaltic pump 6. In this embodiment, the end of the main line 4 downstream of the peristaltic pump 6 is integrally provided with the end cap 31 of the cell culture dish 3.

According to an embodiment of the present invention, a through hole is opened on the end cap 31, an end portion of one end of the main pipeline 4 connected to the end cap 31 is integrally disposed with an edge of the through hole located at an inner side (i.e., a side connected to a mouth of the bottle body 32) or an outer side of the end cap 31, and an end surface of the end portion of the main pipeline 4 does not exceed an inner side surface of the end cap 31.

Through the arrangement, the cell culture dish realizes the airtight construction of the cell culture dish unit by combining the structures of the feeding unit 1, the collecting unit 2, the cell culture dish 3, the pipeline and the like, so that the whole cell culture process can be effectively realized as long as the cleanliness of the whole culture system is ensured, no extra manual work is needed, and the risk that the culture dish is polluted due to manual work is effectively avoided. In addition, the structure of the invention also effectively realizes the automation in the cell culture process, and further effectively improves the culture efficiency.

Through the arrangement, the cover body and the pipeline of the cell culture dish are directly and integrally arranged, so that the problem that the sealing property between the pipeline and the cover body is difficult to solve during system assembly is effectively solved, and the risk that the joint position of the pipeline and the cover body is easily corroded by pollutants is also effectively avoided. In addition, the structure that the pipeline and the cover body are integrally arranged further facilitates the connection with the bottle body of the culture dish, effectively eliminates the contact to the inside of the culture dish in the connection process, greatly ensures the cleanliness of the whole system and is beneficial to ensuring the normal operation of the whole period of the cell culture process.

Through the arrangement, the whole sealing performance is excellent, and the culture dish is beneficial to ensuring the stability and cleanness of the inside of the whole culture dish.

As shown in fig. 1, according to one embodiment of the present invention, a supply unit 1 includes: a plurality of first containers 11 for storing nutrient solution, a plurality of second containers 12 for storing enzymes, a first branch pipe 13 connected with the first containers 11, a second branch pipe 14 connected with the second containers 12, and branch pipe pressure valves 15 respectively arranged on the first branch pipe 13 and the second branch pipe 14. In the present embodiment, on the main pipeline 4, the first branch pipes 13 and the second branch pipes 14 have a spaced staggered arrangement. In the present embodiment, the number of the first container 11 and the second container 12 can be adjusted and matched according to the type of cells to be cultured, so as to meet the culture requirement. In the present embodiment, the type of the enzyme stored in the second container may be set as needed, and for example, the e-enzyme for collection may be selectively stored in the second container 12.

Through the setting, the accurate control of supplying liquid to each container can be realized through setting up the pipe pressure valve on every branch pipe to make the accurate configuration of the nutrient solution of cell culture in-process, guaranteed the normal clear of whole cultivation process.

Through the setting, arrange on the main pipeline with the mode of staggered arrangement through the branch pipe with different kinds of containers to make the liquid in different kinds of containers add the time through the main pipeline simultaneously and can realize the mixture in the pipeline, and then can effectively guarantee that the distribution of different kinds of liquid when getting into the cell culture dish is more even.

As shown in fig. 1, according to one embodiment of the present invention, the collecting unit 2 includes: a plurality of collecting containers 21, collecting branch pipes 22 connected to the collecting containers 21, collecting pipes 23 connected to the main line 4, and collecting pipe pressure valves 24 provided in the collecting branch pipes 22, respectively. In the present embodiment, the collection branch pipe 22 is connected to the collection container 21 at one end and to the collection pipe 23 at the other end. In the present embodiment, the collecting branch pipes 22 are arranged at intervals on the collecting pipe 23. In this embodiment, the number of the collection containers 21 can be set according to the requirement, so as to ensure the flexibility of the present invention in practical application.

According to one embodiment of the present invention, the collection container 21 includes a collection container cover and a collection container body 211 detachably coupled to the collection container cover. In the present embodiment, the end of the collection manifold 22 is provided integrally with the collection container lid. In this embodiment, the end of the collection manifold 22 may be disposed to extend into the collection container lid or may be flush with the inside surface of the collection container lid.

In the present embodiment, the collection container 21 is provided with two containers, one for collecting the culture medium liquid after the culture in the culture dish and the other for collecting the supernatant in the culture dish.

Referring to fig. 1 and 2, according to an embodiment of the present invention, the cell culture dish 3 further includes: a bottle body 32 detachably connected to the end cap 31. In the present embodiment, the bottle body 32 includes: a body portion 321, and a neck portion 322 provided at one side of the body portion 321. In this embodiment, a bottom portion of an end of the body portion 321 connected to the neck portion 322 is formed as an inclined surface 3211, and the remaining portion is a flat surface 3212.

Through the arrangement, the position where the bottleneck portion 322 is connected with the bottle body portion 321 is set to be an inclined plane through the inclined bottle bottom portion, so that the liquid feeding is smooth and beneficial, the liquid is effectively guided to the rear portion of the bottle body 32, and the normal growth of cells at the rear portion in the culture dish is guaranteed.

In this embodiment, the edge of the inclined surface 3211 of the bottle body 32 is provided with a leg 3211 a; the bottom ends of the legs 3211a are flush with the flat surface 3212 of the bottom of the body portion 321.

Through the arrangement, the smoothness of the bottom of the bottle body 32 part is effectively guaranteed, the stable placement of the bottom of the bottle body 32 is facilitated, and the stability of the culture environment is guaranteed.

According to one embodiment of the invention, the body 32 of the cell culture dish 3 is partially transparent. In the present embodiment, a convex lens is provided on the inclined surface 3211 of the vial 32. In the present embodiment, the convex lens is provided integrally with the inclined surface 3211. Through set up convex lens on the inclined plane, the effectual realization is counted and is observed the quantity of cell on the inclined plane, and the cell growth state of the non-horizontal bottom surface position of judgement that can be accurate has guaranteed the accurate grasp to whole culture dish internal culture environment.

According to an embodiment of the invention, a convex lens device may also be provided on the non-inclined surface portion of the bottom and/or the top of the cell culture dish 3. In this embodiment, the convex lens device includes a turntable, and a plurality of convex lenses provided at intervals in a circumferential direction on the turntable. In the present embodiment, the magnification of each convex lens is different. In this embodiment, the carousel detachable installs on cell culture dish 3, can realize that different multiplying powers convex lens monitor statistics to culture dish same position cell growth state or culture dish internal environment through rotatory carousel.

Through set up convex lens in different positions on the culture dish, effectual realization is to the direct observation of inside cell normal growth state. Especially, the variable-magnification convex lens device can further realize the detection of different magnifications in different positions in the circumferential direction, further ensures the comprehensive control of the environment inside the culture dish, and is beneficial to ensuring the final cell culture effect.

According to an embodiment of the present invention, the rotary plate of the convex lens device may be configured as an electric rotary plate, and the rotation may be realized by the control of the control signal, so as to ensure the accuracy of the rotation position.

According to one embodiment of the invention, the end cap 31 or the bottle body 32 of the cell culture dish 3 is provided with a medical air-permeable filter membrane for air permeation and liquid blocking. In this embodiment, the medical gas permeable filter membrane can only allow gas to pass through, so as to ensure the exchange between the gas in the culture dish and the gas outside the culture dish. The medical air-permeable filter membrane can prevent other substances such as moisture, bacteria and the like from passing through so as to ensure the maintenance of culture solution in the culture dish and play a role of cleaning air.

As shown in fig. 2, according to one embodiment of the present invention, a light sensing calibration device a is disposed on a sidewall of the bottle body 32. Whether the calibration device that whether cell pollutes in the detection culture dish as the culture process through set up light sense calibration device A on the lateral wall at the culture dish can cooperate the sensor to use, improves the promptness to the internal culture environment detects.

Referring to fig. 1, 3, 4 and 5, according to an embodiment of the present invention, a cell culture dish with a liquid supply and drainage pipeline of the present invention further includes: a culture dish carrier 7 for carrying the cell culture dish 3. In the present embodiment, the culture dish holder 7 includes: a base 71, a lifting seat 72 and a driving device 73. In the present embodiment, the driving device 73 is supported by the base 71. One end of the lifting base 72 is a rotation connection end rotatably connected to the base 71, and the other end thereof is a lifting connection end connected to the driving device 73. In the present embodiment, the elevating connection end can move up and down relative to the base 71 under the driving action of the driving device 73.

Through the arrangement, the adherent cell culture dish is borne by the lifting seat 72, and the lifting connecting end of the lifting seat can be rotated to vertically lift through the action of the driving device, so that the lifting seat 72 generates inclined motion relative to the base 71, liquid in the culture dish smoothly flows out when needing to be replaced, and residues in the culture dish are avoided.

Through the arrangement, the lifting operation of the lifting seat can be automatically realized, manual participation is not required, and the updating efficiency of the liquid in the culture dish can be effectively improved.

Referring to fig. 3 and 6, according to an embodiment of the present invention, the base 71 includes: a frame 711, and a bottom support 712 provided on the lower side of the frame 711. In the present embodiment, the frame 711 is a continuous rectangular frame, and the size thereof can be adjusted adaptively according to the installation position. In the present embodiment, the bottom support 712 is a plate-like body, and is fixedly attached to the lower side of the frame body 711. In the present embodiment, the bottom support 712 and the frame 711 may be integrally provided, or may be fixed by means of adhesion, screwing, or the like.

In the present embodiment, a plurality of legs are provided at intervals on the lower side of the housing 711.

Referring to fig. 4 and 7, according to an embodiment of the present invention, the elevating base 72 is located inside the frame 711. And above the bottom support 712, in the present embodiment, the rotation shafts 72a for rotatably connecting with the frame 711 are provided on opposite sides of the rotation connection end of the elevating base 72. In the present embodiment, the rotation shaft 72a is connected to the elevating base 72 and the housing 711 via a bearing, and the elevating base 72 rotates with respect to the housing 711. In the present embodiment, in order to connect the elevating base 72 to the inside of the frame 711, the shape of the elevating base 72 is matched with the shape of the inside of the frame 711, and if the inside of the frame 711 is rectangular, the shape of the elevating base 72 is rectangular.

Referring to fig. 4 and 7, according to an embodiment of the present invention, the lifting base 72 is a frame structure having a mounting position 72b for mounting a culture dish. In the present embodiment, the elevation base 72 is a hollow frame structure, and the mounting position 72b for the petri dish thereon is formed by the hollow structure of the elevation base 72. Specifically, the mounting position 72b includes: a first hollow portion 72b1 and a second hollow portion 72b2 communicating with each other. In the present embodiment, the second hollow part 72b2 and the first hollow part 72b1 are sequentially arranged in a direction from the rotation connection end to the elevation connection end of the elevation base 72 (i.e., the length direction), wherein the second hollow part 72b2 has an opening at the end of the rotation connection end of the elevation base 72. In this embodiment, the widths of the first hollow part 72b1 and the second hollow part 72b2 are different, so that a step structure is formed on the mounting position 72b, which is favorable for mounting and positioning the culture dish in the first hollow part 72b1, and ensures the mounting stability of the culture dish, and the second hollow part 72b2 corresponds to the bottle neck and the bottle cap on the culture dish, so that the whole culture dish is stably mounted.

In this embodiment, the second hollow portion 72b2 forms an opening at the end of the rotation connection end, which can further avoid the interference with the structure of the culture dish, such as the bottle neck, the bottle cap, etc., and can adapt to the installation of the bottle neck and the bottle cap with different sizes, and provide sufficient space for avoiding the interference of the whole culture dish bearing platform 7 and the culture dish during the lifting process of the lifting seat 72.

Through the setting, the installation position 72b is set to be of a hollow structure, so that the position of interference with the culture dish can be effectively reduced, and the stable installation of the culture dish is facilitated. In addition, the installation position 72b is hollow, so that the quality of the lifting seat can be greatly reduced, the structure of the lifting seat is simplified, and the long-time stable operation of the lifting seat is ensured. In addition, the hollow mounting position 72b is also beneficial to mounting other optical equipment on the culture dish bearing platform 7 of the invention, and is used for observing the state in the culture dish in real time, thereby effectively improving the use flexibility of the invention.

In this embodiment, the lower side of the end of the rotation connection end of the lifting base 72 is provided with an arc surface for connecting the end surface and the lower side of the rotation connection end, thereby forming a transition portion at the end position.

Through the arrangement, the arc surface is arranged on the lower side of the end part of the rotating connecting end of the lifting seat 72, so that the smooth rotation of the lifting seat 72 is favorably ensured, and the use stability of the lifting seat is further ensured.

Referring to fig. 1 and 3, according to an embodiment of the present invention, the elevating base 72 is provided with light-transmitting passages 721 penetrating through at opposite sides of the mounting position 72b in the width direction of the elevating base 72; in the present embodiment, the light transmitting passage 721 is provided at a position of the first hollow portion 72b1 near the elevating connection end of the elevating base 72. In this embodiment, the light transmission channel 721 is coaxially arranged on the frame of the lifting seat 72, and then, when the culture dish is fixed, the light transmission channel 721 can be coaxially arranged on two opposite sides of the culture dish, and then, the light transmission channel 721 on one side is polished, and the light transmission channel 721 on the other side can be reached after passing through the culture dish and transmitted out, so that the internal state (such as internal environment, cell growth state, pollution condition, nutrient solution turbidity degree, and the like) of the culture dish can be collected, and the subsequent detection and analysis can be facilitated.

In the present embodiment, the light transmission channel 721 is disposed near the lower side of the elevating base 72 in the thickness direction of the elevating base 72, i.e., the distance between the light transmission channel 721 and the lower side is smaller than the distance between the connection channel and the upper side.

Through the above arrangement, the light transmission channel 721 is arranged at the position close to the lifting connecting end of the lifting seat 72, so that the internal environment at the rear part of the culture dish can be detected in real time, and the accurate detection of the internal environment of the culture dish can be mastered.

Referring to fig. 4 and 7, a support structure 722 is provided at the bottom of the lift seat 72 according to an embodiment of the present invention. In this embodiment, the support structure 722 includes: a first support structure 7221 and a second support structure 7222. The first support structure 7221 is disposed along the inside edge of the bottom of the first hollow portion 72b 1; the second support structure 7222 is connected to portions of the inside edges of the bottom of the second hollow portion 72b2 on opposite sides thereof. In the present embodiment, the first support structure 7221 is an elongated structure that is continuously or intermittently provided at the inner side edge of the bottom of the first hollow portion 72b 1. In this embodiment, the second support structure 7222 is a plate-like body having opposite ends fixedly connected to the inner side edges of the portions of the second hollow portion 72b2 on the opposite sides of the bottom thereof, respectively.

With the above arrangement, the support structure 722 is disposed at the bottom edge of the mounting position 72b to effectively support the edge of the culture dish and the neck portion.

In this embodiment, the second support structure 7222 is spaced from the end opening of the rotary connection end.

With the above arrangement, the space between the second support structure 7222 and the end opening of the rotary connection end can play a role of avoiding, avoiding interference with other structures, and being beneficial to ensuring normal and stable operation of the present invention.

Referring to fig. 3, 4, 5 and 8, according to an embodiment of the present invention, the driving device 73 includes: a driver 731 for outputting a linear displacement, and a yoke 732 connected to the driver 731. In the present embodiment, the yoke 732 includes: a driver connecting arm 7321 and a lifting base connecting arm 7322; the lifting base connecting arm 7322 is disposed perpendicular to the driver connecting arm 7321. In this embodiment, the actuator connecting arm 7321 and the lifter base connecting arm 7322 may be integrally provided, or may be connected by a screw, an adhesive, a caulking, or the like. In this embodiment, two lifter connecting arms 7322 are provided, and are connected to the driver connecting arm 7321 in parallel.

Referring to fig. 4, 5, 6 and 9, according to one embodiment of the invention, a linear channel 7121 is provided in the base support 712. In this embodiment, the end of the lifting base connecting arm 7322 away from the driver connecting arm 7321 is hinged to the lifting connecting end of the lifting base 72 through the linear channel 7121. In this embodiment, a hinge seat for connecting with the driver connecting arm 7321 is disposed below the elevating connecting end, the hinge seat is formed by two parallel plate-like bodies disposed at an interval, and the connection can be completed by sequentially passing through the hinge seat and the corresponding mounting positions on the driver connecting arm 7321 through the hinge shaft.

In this embodiment, the lifting base connecting arm 7322 is located at the lower side of the bottom support 712, and the side away from the driver connecting arm 7321 is hinged with the movable end of the driver 731. In this embodiment, a first notch is formed in the bottom support 712 below the rotation connection end of the elevating base 72, and a second notch is formed in the frame 711 of the base 71 at a position corresponding to the first notch, the second notch is communicated with the first notch, and the driving device 73 can be installed at the positions of the first notch and the second notch.

In this embodiment, the driver 731 is a linear driving device, and the telescopic end of the driver 731 can move in a telescopic manner along a linear direction, so that the free end of the driver 731 is hinged to the driver connecting arm 7321, thereby pushing the lifting seat 72.

According to an embodiment of the present invention, the actuator 731 is implemented by an electric cylinder, which can precisely control the displacement of the telescopic end thereof through an electric signal, and can automatically and precisely control the inclination angle of the lifting seat through the control of the electric signal, so as to achieve the micro-flow of the nutrient solution in the culture dish and the flexible adjustment of the horizontal position of the nutrient solution.

Through the above arrangement, the driver connecting arm 7321 is arranged at the lower side of the bottom support 712, and the lifting seat 72 is located at the upper side of the bottom support 712, and in the process of lifting and lowering the lifting seat 72 by the connection between the driver 731 and the driver connecting arm 7321, the bottom support 712 can support and limit the driving arm 7321, thereby facilitating the stable operation of the driver 731. In addition, through the above arrangement, the accurate control of the inclination angle of the lifting seat 72 can be flexibly realized by controlling the telescopic length of the free end of the driver 731 without other complex structures, and the advantages of high operation precision, good stability, simple structure and the like are realized.

In this embodiment, the hinge position of the driver connecting arm 7321 and the driver 731 is provided with a rubber damping structure for silent rotation of the hinge position. In this embodiment, the accessible sets up the damping structure that rubber was done on articulated position's both sides or articulated shaft to the realization is to articulated position's amortization, but also the effectual smooth and easy stability of having guaranteed the operation and be useful to guaranteeing the accuracy of operating position.

In the present embodiment, a position sensor for detecting the movement position of the driver connecting arm 7321 is embedded. The position sensor can detect the position of the driver connecting arm 7321 in real time, so that the position of the yoke 732 can be accurately controlled, and the inclination angle of the lifting seat can be accurately controlled.

In the present embodiment, the actuator connecting arm 7321 is further provided with a stopper structure for limiting the maximum displacement of the actuator connecting arm 7321. Excessive movement of the driver connecting arm 7321 can be effectively avoided by changing the limiting structure, so that the maximum rising height of the lifting seat 72 can be effectively suppressed, and further, stable operation of the lifting seat is ensured, and interference with other structures is eliminated.

Referring to fig. 3 and 9, according to an embodiment of the present invention, a detection device 74 for detecting an internal state of the culture dish mounted on the lifting seat 72 is provided on the frame 711. In the present embodiment, the detection device is a correlation detection device. Specifically, the detection device 74 includes: laser light source 741, optical fiber 742, optical receiver 743. In this embodiment, mounting through holes penetrating the housing are provided on both sides of the housing 711 facing each other, and the laser light source 741 and the optical receiver 743 can be mounted in the mounting through holes facing each other. The optical fibers 742 are respectively embedded in the light-transmitting channels 721 of the lifting base 72; when the lifting seat 72 is horizontally positioned in the frame 711, the laser light source 741, the optical fiber 742 and the optical receiver 743 are aligned, light emitted by the laser light source 741 can be transmitted to the optical receiver 743 through the optical fiber 742, so that the environment in the culture dish can be detected, and when the lifting seat 72 is inclined, the lifting connection end is separated from the frame 711, so that the detection device 74 can output a lifting signal of the lifting seat 72, and the environment in the culture dish cannot be detected. In this embodiment, the optical receiver 743 can process and analyze the received signal by itself, or transmit the signal to another device for processing and analysis, and the processing and analysis can be set according to practical applications.

Through the arrangement, the automatic detection of the interior of the culture dish can be realized by adopting the detection device to detect the culture dish. In addition, automatic control of the lifting seat can be realized through on-off of the detection device, and the control accuracy of the lifting seat is improved.

In addition, the optical fiber 742 can be abutted against the culture dish according to the requirement, so that a certain pre-tightening effect can be provided for the culture dish, the reliable fixation of the culture dish is beneficial, the arrangement mode is more beneficial when the lifting seat is in an inclined state, and the problem that the culture dish is turned over due to the accumulation of liquid in the culture dish at the bottleneck end can be effectively solved through the abutting pre-tightening effect on the rear part of the culture dish, so that the operation of the invention is more reliable. In addition, the optical fiber 742 may be integrated with the culture dish to further fix the culture dish while eliminating the influence of the contact position on the detection result.

As shown in fig. 6, according to an embodiment of the present invention, a first mounting position 712a penetrating through the bottom support 712 is provided on the bottom support 712, and a focusing device, a lens, etc. for an imaging device can be mounted through the first mounting position 712a, so as to achieve image capture of the imaging device. In the present embodiment, the first mounting location 712a is located below the first hollow portion 72b1 of the mounting location 72 b.

In order to further explain the invention, the working flow of the invention is further explained with reference to the attached drawings.

S1, respectively filling nutrient solution and cells to be cultured into a first container 11 and a second container 12 according to preset settings, and connecting the first container 11, the second container 12, a cell culture dish 3 and a collection container 21; wherein, the first pipe pressure valve 5, the branch pipe pressure valve 15 and the collecting pipe pressure valve 24 are all in a state of pressing the pipeline;

s2, respectively controlling the first pipe pressure valve 5 and the branch pipe pressure valve 15 to be opened, starting the peristaltic pump 6 to act so as to realize the addition of the cells to be cultured and the nutrient solution in the cell culture dish 3, and closing the first pipe pressure valve 5, the branch pipe pressure valve 15 and the peristaltic pump 6 after the addition is finished;

s3, after the cell culture in the cell culture dish 3 is finished, the lifting platform in the culture dish bearing platform 7 is lifted, the pipe pressure valve 24 of the collecting pipe is opened, and the peristaltic pump 6 acts to discharge the waste liquid in the cell culture dish 3 to the collecting container 21.

According to an embodiment of the present invention, in step S3, if the cells in the cell culture dish 3 need to be refreshed during the culture process, the method comprises the following steps:

the lifting platform in the culture dish bearing platform 7 is lifted, the pipe pressure valve 24 of the collecting pipe is opened, and the peristaltic pump 6 acts to discharge the waste liquid in the cell culture dish 3 into the collecting container 21;

the lifting platform returns to the original position, and the pipe pressure valve 24 of the collecting pipe and the peristaltic pump 6 stop running;

control first pipe pressure valve 5, branch pipe pressure valve 15 respectively and open, peristaltic pump 6 action of restarting to realize waiting to cultivate cell and nutrient solution and add in cell culture dish 3, and close first pipe pressure valve 5, branch pipe pressure valve 15 and peristaltic pump 6 after accomplishing to add.

According to an embodiment of the present invention, during the cell culture in the cell culture dish 3, the slow flow of the liquid in the culture dish can be realized by controlling the slow lifting of the lifting platform in the culture dish bearing platform 7, so as to remix the nutrient solution, thereby eliminating the imbalance phenomenon of cell growth.

The foregoing is merely exemplary of particular aspects of the present invention and devices and structures not specifically described herein are understood to be those of ordinary skill in the art and are intended to be implemented in such conventional ways.

The above description is only one embodiment of the present invention, and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.

Claims

1. A cell culture dish unit with a liquid supply and discharge pipeline, characterized in that it comprises: a feeding unit (1), a collection unit (2), a cell culture dish (3), a main pipeline (4), a first Pipe pressure valve (5) and peristaltic pump (6);

The first pipe pressure valve (5) and the peristaltic pump (6) are arranged on the main pipeline (4) at intervals;

The feeding unit (1) is connected to the main pipeline (4), and the connection position of the feeding unit (1) and the main pipeline (4) is located at the first pipe pressure valve (5) upstream;

The collection unit (2) is connected with the main pipeline (4), and the connection position of the collection unit (2) and the main pipeline (4) is located at the first pipe pressure valve (5) and the main pipeline (4). between the peristaltic pumps (6);

The end of the main pipeline (4) located downstream of the peristaltic pump (6) is integrally provided with the end cover (31) of the cell culture dish (3), and the end face of the end does not exceed the end cover (31) inner side.

2. The cell culture dish unit according to claim 1, wherein the feeding unit (1) comprises: a plurality of first containers (11) for storing nutrient solution, a plurality of first containers (11) for storing enzymes The second container (12), the first branch pipe (13) connected with the first container (11), and the second branch pipe (14) connected with the second container (12), are respectively in the a branch pipe (13) and a branch pipe pressure valve (15) provided on the second branch pipe (14);

On the main pipeline (4), the first branch pipes (13) and the second branch pipes (14) have a spaced staggered arrangement.

3. The cell culture dish unit according to claim 2, wherein the collection unit (2) comprises: a plurality of collection containers (21), a collection branch pipe (22) connected to the collection container (21) ) for the collecting pipe (23) connected to the main pipeline (4), and the collecting pipe pipe pressure valve (24) respectively provided on the collecting branch pipe (22);

One end of the collection branch pipe (22) is connected with the collection container (21), and the other end is connected with the collection pipe (23);

The collection container (21) comprises: a collection container cover and a collection container body (211) detachably connected with the collection container cover;

The end of the collecting branch pipe (22) is integrally arranged with the collecting container cover.

4. The cell culture dish unit according to claim 3, wherein the cell culture dish (3) further comprises: a bottle body (32) detachably connected to the end cap (31);

The bottle body (32) comprises: a bottle body part (321) and a neck part (322) provided on one side of the bottle body part (321);

The bottom part of one end of the bottle body part (321) connected with the neck part (322) is set as an inclined surface (3211), and the rest part is a flat surface (3212);

The edge of the inclined surface (3211) is provided with feet (3211a);

The bottom end of the foot (3211a) is flush with the plane (3212) of the bottom of the bottle body part (321);

The end cap (31) or the bottle body (32) is provided with a breathable and liquid-resistant medical breathable filter membrane.

5. The cell culture dish unit according to any one of claims 1 to 4, characterized in that, further comprising: a culture dish bearing platform (7);

The culture dish carrying platform (7) comprises: a base (71), a lift seat (72), a driving device (73) and a detection device (74) for detecting the internal state of the culture dish installed on the lift seat (72). );

the drive device (73) is supported on the base (71);

One end of the lifting seat (72) is a rotating connection end rotatably connected with the base (71), and the other end is a lifting connection end connected with the driving device (73);

Under the driving action of the driving device (73), the lifting connecting end can move up and down relative to the base (71);

The bottle body (32) of the cell culture dish (3) is detachably mounted on the lifting seat (72).

6. The cell culture dish unit according to claim 5, wherein the base (71) comprises: a frame body (711) and a bottom support (712) disposed on the lower side of the frame body (711);

The lift seat (72) is located inside the frame body (711) and above the bottom support (712);

Rotating shafts (72a) for rotational connection with the frame body (711) are respectively provided on opposite sides of the rotating connection end of the lifting seat (72).

7. The cell culture dish unit according to claim 6, wherein the lifting seat (72) is a frame structure, and has an installation position (72b) for installing the cell culture dish (3);

The installation position (72b) includes: a first hollow part (72b1) and a second hollow part (72b2) that communicate with each other;

The second hollow portion (72b2) has an opening at the end of the rotating connection end of the lift seat (72);

The bottle body (32) of the cell culture dish (3) is located in the first hollow part (72b1), and the neck part (322) of the cell culture dish (3) is located in the second hollow part (72b2) middle;

In the width direction of the lifting seat (72), the lifting seat (72) is provided with light-transmitting channels (721) passing through on opposite sides of the installation position (72b);

The light transmission channel (721) is arranged at a position of the first hollow portion (42b1) close to the lift connection end of the lift seat (72).

8. The cell culture dish unit according to claim 7, wherein a support structure (723) is provided at the bottom of the lift seat (72);

The support structure (723) includes: a first support structure (7231) and a second support structure (7232);

The first support structure (7231) is arranged along the inner edge of the bottom of the first hollow portion (72b1);

The second support structure (7232) is connected to part of the inner side edges on opposite sides of the bottom of the second hollow portion (72b2).

9. The cell culture dish unit according to claim 8, characterized in that, the first support structure (7231) is an elongated structure, which is continuous at the inner edge of the bottom of the first hollow portion (72b1) or Intermittent settings;

The second support structure (7232) is a plate-shaped body, and its opposite ends are respectively fixedly connected to part of the inner side edges of the opposite sides of the bottom of the second hollow portion (72b2);

The second support structure (7232) is spaced apart from the end opening of the rotational connection end.

10. The cell culture dish unit according to claim 9, wherein the driving device (73) comprises: a driver (731) for outputting linear displacement, a fork arm connected with the driver (731) (732);

The fork arm (732) includes: a driver connecting arm (7321) and a lifting seat connecting arm (7322);

The lifting seat connecting arm (7322) is perpendicular to the driver connecting arm (7321);

A linear channel (7121) is arranged on the bottom support (712);

One end of the lifting seat connecting arm (7322) away from the driver connecting arm (7321) is hinged with the lifting connecting end of the lifting seat (72) through the linear channel (7121);

The lifting seat connecting arm (7322) is located on the lower side of the bottom support (712), and the side away from the driver connecting arm (7321) is hinged with the movable end of the driver (731);

A rubber damping structure is provided at the hinged position of the driver connecting arm (7321) and the driver (731) for silent rotation of the hinged position;

The driver connecting arm (7321) is embedded with a position sensor for detecting its moving position, and a limiting structure for limiting the maximum displacement of the driver connecting arm (7321).