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CN113574173A - 突变的羟基苯丙酮酸双加氧酶多肽、其编码基因及用途 - Google Patents

突变的羟基苯丙酮酸双加氧酶多肽、其编码基因及用途 Download PDF

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CN113574173A
CN113574173A CN201980094222.5A CN201980094222A CN113574173A CN 113574173 A CN113574173 A CN 113574173A CN 201980094222 A CN201980094222 A CN 201980094222A CN 113574173 A CN113574173 A CN 113574173A
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nucleotide sequence
seq
plant
amino acid
arabidopsis thaliana
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肖翔
陶青
于彩虹
宋庆芳
鲍晓明
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Beijing Dabeinong Biotechnology Co Ltd
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Abstract

提供了一种突变的羟基苯丙酮酸双加氧酶多肽、其编码基因及用途，所述突变的羟基苯丙酮酸双加氧酶多肽保留催化对羟基苯丙酮酸转化为尿黑酸或尿黑酸盐的活性，且对HPPD抑制剂除草剂的敏感性低于原始的未突变HPPD，其在对应于SEQ ID NO:1所示氨基酸序列的372位点上包含如下突变：F372A、F372G、F372V、F372P、F372S、F372T、F372C、F372M、F372Q、F372D或F372缺失。所述突变体可以赋予植物对HPPD抑制剂除草剂表现出较高的耐受性，可用于培育耐受HPPD抑制剂除草剂的植物。

Description

PCT国内申请，说明书已公开。

Claims (16)

  1. PCT国内申请，权利要求书已公开。
CN201980094222.5A 2019-09-17 2019-09-17 突变的羟基苯丙酮酸双加氧酶多肽、其编码基因及用途 Pending CN113574173A (zh)

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AR (1) AR119996A1 (zh)
AU (1) AU2019466501B2 (zh)
BR (1) BR112021024656A2 (zh)
CA (1) CA3154740A1 (zh)
IL (1) IL291089A (zh)
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Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111334484A (zh) * 2020-03-19 2020-06-26 北京大北农生物技术有限公司 除草剂耐受性蛋白质、其编码基因及用途
CN114181956A (zh) * 2022-01-26 2022-03-15 西南大学 小麦条锈病抗性相关代谢物及其合成相关基因与应用
CN114585731A (zh) * 2021-12-15 2022-06-03 北京大北农生物技术有限公司 突变的羟基苯丙酮酸双加氧酶多肽、其编码基因及用途
WO2022127894A1 (zh) * 2020-12-17 2022-06-23 上海蓝十字医学科学研究所 除草剂抗性植物
CN116064430A (zh) * 2021-09-14 2023-05-05 山东舜丰生物科技有限公司 突变的hppd多肽及其应用

Families Citing this family (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114317481B (zh) * 2022-02-08 2023-10-27 宁夏农林科学院农作物研究所（宁夏回族自治区农作物育种中心） 水稻als突变型基因及其蛋白在抗除草剂方面的应用

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101998993A (zh) * 2008-04-14 2011-03-30 拜耳生物科学股份有限公司 新的突变羟基苯基丙酮酸双加氧酶，dna序列和耐受hppd抑制剂除草剂的植物分离
CN102348802A (zh) * 2009-01-22 2012-02-08 先正达参股股份有限公司 突变体羟基苯丙酮酸双加氧酶多肽及其使用方法
CN106459986A (zh) * 2014-03-11 2017-02-22 拜耳作物科学有限合伙公司 Hppd变体和使用方法
CN107075488A (zh) * 2014-03-11 2017-08-18 拜耳作物科学股份公司 Hppd变体及使用方法

Family Cites Families (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU590597B2 (en) 1985-08-07 1989-11-09 Monsanto Technology Llc Glyphosate-resistant plants
ATE57390T1 (de) 1986-03-11 1990-10-15 Plant Genetic Systems Nv Durch gentechnologie erhaltene und gegen glutaminsynthetase-inhibitoren resistente pflanzenzellen.
US5276268A (en) 1986-08-23 1994-01-04 Hoechst Aktiengesellschaft Phosphinothricin-resistance gene, and its use
US4873192A (en) 1987-02-17 1989-10-10 The United States Of America As Represented By The Department Of Health And Human Services Process for site specific mutagenesis without phenotypic selection
US4940935A (en) 1989-08-28 1990-07-10 Ried Ashman Manufacturing Automatic SMD tester
US5994629A (en) 1991-08-28 1999-11-30 Novartis Ag Positive selection
GB9304200D0 (en) 1993-03-02 1993-04-21 Sandoz Ltd Improvements in or relating to organic compounds
US20020058249A1 (en) 1998-08-12 2002-05-16 Venkiteswaran Subramanian Dna shuffling to produce herbicide selective crops
US7462481B2 (en) 2000-10-30 2008-12-09 Verdia, Inc. Glyphosate N-acetyltransferase (GAT) genes
US7405074B2 (en) 2004-04-29 2008-07-29 Pioneer Hi-Bred International, Inc. Glyphosate-N-acetyltransferase (GAT) genes
CL2008001592A1 (es) * 2007-05-30 2009-03-06 Syngenta Participations Ag Celula vegetal que comprende una construccion de acido nucleico que contine una secuencia nucleotidica que codifica un citocromo p450, y metodo para conferir resistencia a un herbicida en la planta basado en la transformacion de la construccion descrita anteriormente.
US9347046B2 (en) * 2009-01-22 2016-05-24 Syngenta Participations Ag Hydroxyphenylpyruvate dioxygenase polypeptides and methods of use
EP2669369A1 (en) * 2010-11-10 2013-12-04 Bayer CropScience AG HPPD variants and methods of use
WO2012080975A1 (en) * 2010-12-16 2012-06-21 Basf Se Plants having increased tolerance to herbicides
CA2808152C (en) * 2013-03-05 2023-10-24 Bayer Cropscience Ag Hppd variants and methods of use
WO2014177992A2 (en) * 2013-04-30 2014-11-06 Basf Se Plants having increased tolerance to herbicides
BR112015027484A2 (pt) * 2013-04-30 2017-12-05 Basf Se método para controlar vegetação indesejada, método para identificar uma bicicloarilcarboxamida, método para identificar uma sequência de nucleotídeos, ácidos nucleicos, célula vegetal, plantas, semente, método para produzir uma célula vegetal, método para produzir uma planta e método para identificar ou selecionar uma célula
WO2014177999A2 (en) * 2013-04-30 2014-11-06 Basf Se Plants having increased tolerance to herbicides
CA2947032A1 (en) * 2014-05-02 2015-11-05 Pioneer Hi-Bred International, Inc. Compositions and methods comprising sequences having hydroxyphenylpyruvate dioxygenase (hppd) activity

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101998993A (zh) * 2008-04-14 2011-03-30 拜耳生物科学股份有限公司 新的突变羟基苯基丙酮酸双加氧酶，dna序列和耐受hppd抑制剂除草剂的植物分离
CN102348802A (zh) * 2009-01-22 2012-02-08 先正达参股股份有限公司 突变体羟基苯丙酮酸双加氧酶多肽及其使用方法
CN106459986A (zh) * 2014-03-11 2017-02-22 拜耳作物科学有限合伙公司 Hppd变体和使用方法
CN107075488A (zh) * 2014-03-11 2017-08-18 拜耳作物科学股份公司 Hppd变体及使用方法

Cited By (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111334484A (zh) * 2020-03-19 2020-06-26 北京大北农生物技术有限公司 除草剂耐受性蛋白质、其编码基因及用途
CN111334484B (zh) * 2020-03-19 2022-06-03 北京大北农生物技术有限公司 除草剂耐受性蛋白质、其编码基因及用途
WO2022127894A1 (zh) * 2020-12-17 2022-06-23 上海蓝十字医学科学研究所 除草剂抗性植物
CN116064430A (zh) * 2021-09-14 2023-05-05 山东舜丰生物科技有限公司 突变的hppd多肽及其应用
CN116064430B (zh) * 2021-09-14 2023-08-11 山东舜丰生物科技有限公司 突变的hppd多肽及其应用
CN114585731A (zh) * 2021-12-15 2022-06-03 北京大北农生物技术有限公司 突变的羟基苯丙酮酸双加氧酶多肽、其编码基因及用途
WO2023108495A1 (zh) * 2021-12-15 2023-06-22 北京大北农生物技术有限公司 突变的羟基苯丙酮酸双加氧酶多肽、其编码基因及用途
CN114181956A (zh) * 2022-01-26 2022-03-15 西南大学 小麦条锈病抗性相关代谢物及其合成相关基因与应用
CN114181956B (zh) * 2022-01-26 2023-11-28 西南大学 小麦条锈病抗性相关代谢物及其合成相关基因与应用

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IL291089A (en) 2022-07-01
EP4032976A1 (en) 2022-07-27
BR112021024656A2 (pt) 2022-04-12
AU2019466501B2 (en) 2024-07-25
EP4032976A4 (en) 2023-09-27
US20230212587A1 (en) 2023-07-06
AR119996A1 (es) 2022-01-26
UY38884A (es) 2020-11-30
CA3154740A1 (en) 2021-03-25
AU2019466501A1 (en) 2022-02-17
AU2019466501A9 (en) 2022-05-05
WO2021051265A1 (zh) 2021-03-25

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