CN113528386A - Preparation method of aquatic product composite microecological preparation - Google Patents
Preparation method of aquatic product composite microecological preparation Download PDFInfo
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- CN113528386A CN113528386A CN202110796918.1A CN202110796918A CN113528386A CN 113528386 A CN113528386 A CN 113528386A CN 202110796918 A CN202110796918 A CN 202110796918A CN 113528386 A CN113528386 A CN 113528386A
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- 238000002360 preparation method Methods 0.000 title claims abstract description 42
- 239000002131 composite material Substances 0.000 title claims abstract description 24
- 241000194108 Bacillus licheniformis Species 0.000 claims abstract description 15
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 15
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 15
- 241000190950 Rhodopseudomonas palustris Species 0.000 claims abstract description 15
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 11
- 238000000034 method Methods 0.000 claims abstract description 7
- 239000002994 raw material Substances 0.000 claims abstract description 5
- 241000894006 Bacteria Species 0.000 claims description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 13
- 229910001868 water Inorganic materials 0.000 claims description 13
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 12
- 239000002054 inoculum Substances 0.000 claims description 10
- 238000003756 stirring Methods 0.000 claims description 10
- 238000012258 culturing Methods 0.000 claims description 8
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 6
- 239000008103 glucose Substances 0.000 claims description 6
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 6
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 6
- 239000011780 sodium chloride Substances 0.000 claims description 6
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 6
- 229960001763 zinc sulfate Drugs 0.000 claims description 6
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 6
- 238000012371 Aseptic Filling Methods 0.000 claims description 4
- 241000235070 Saccharomyces Species 0.000 claims description 4
- 238000011049 filling Methods 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 150000001875 compounds Chemical class 0.000 claims description 3
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 3
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 claims description 2
- IPCXNCATNBAPKW-UHFFFAOYSA-N zinc;hydrate Chemical compound O.[Zn] IPCXNCATNBAPKW-UHFFFAOYSA-N 0.000 claims description 2
- 241001465754 Metazoa Species 0.000 description 6
- 238000009360 aquaculture Methods 0.000 description 5
- 244000144974 aquaculture Species 0.000 description 5
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 3
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 3
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 229940024606 amino acid Drugs 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 235000020958 biotin Nutrition 0.000 description 1
- 239000011616 biotin Substances 0.000 description 1
- 229960002685 biotin Drugs 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
- A23K10/18—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions of live microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Virology (AREA)
- Food Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Tropical Medicine & Parasitology (AREA)
- Mycology (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Animal Husbandry (AREA)
- Physiology (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Insects & Arthropods (AREA)
- Marine Sciences & Fisheries (AREA)
- Birds (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Purification Treatments By Anaerobic Or Anaerobic And Aerobic Bacteria Or Animals (AREA)
Abstract
The invention discloses a preparation method of an aquatic product composite microecological preparation, which relates to the technical field of microecological preparations, and the aquatic product composite microecological preparation comprises the following raw materials: the mass ratio of the bacillus subtilis to the bacillus licheniformis to the rhodopseudomonas palustris to the spherical yeast is 2:2:1:1, and the method for preparing the aquatic product composite microecological preparation comprises the steps of preparing a culture solution, and preparing the aquatic product composite microecological preparation by using the technical scheme of the invention.
Description
Technical Field
The invention relates to the technical field of microecologics, in particular to a preparation method of an aquatic product composite microecologics.
Background
The aquaculture area of China reaches more than 430 million hectares, and due to high-density aquaculture, a large amount of feed needs to be added into a water body in the aquaculture process, feed residues and metabolites of aquaculture animals can cause turbidity of the water body and increase of COD; the decomposition of organic matters such as a large amount of feed residues, excrement and urine in the water body can cause the oxygen content of the water body to reduce, lead to the aquaculture water environment to worsen, influence the healthy growth of aquatic organisms, from this need the microecological preparation to improve the water body, current microecological preparation product type is too single, can not improve the water body to lead to who's breed the reduction of survival rate of aquatic animals.
Disclosure of Invention
The invention aims to solve the problems and designs a preparation method of an aquatic product composite microecological preparation.
The technical scheme of the invention is that the aquatic product composite microecological preparation method comprises the following raw materials: the aquatic product composite micro-ecological preparation comprises bacillus subtilis, bacillus licheniformis, rhodopseudomonas palustris, spherical yeast and a culture solution, wherein the mass ratio of the bacillus subtilis to the bacillus licheniformis to the rhodopseudomonas palustris to the spherical yeast is 2:2:1:1, and the preparation method of the aquatic product composite micro-ecological preparation comprises the following steps:
the culture solution comprises glucose, sodium chloride, potassium dihydrogen phosphate, zinc sulfate and water.
Step 1: respectively inoculating inclined planes of 4 bacteria in a small shake flask containing a culture solution by using inoculating loops under the aseptic operation condition, and culturing for 48 hours at the temperature of 27-29 ℃ under the stirring frequency of 46 Hz;
step 2: transferring 4 qualified bacteria cultured in the small shake flask into a large shake flask containing a culture solution under aseptic conditions, and culturing at 27-29 deg.C under stirring frequency of 35Hz for 72-90 hr;
and step 3: transferring the seed bacteria in the large shake flask in the step 2 into a mixing flask under the aseptic operation condition, wherein the temperature is 27-37 ℃, the rotating speed of a stirrer is 110-;
and 4, step 4: and (4) detecting the mixed inoculum obtained in the step (3), and filling the detected inoculum in an aseptic filling room.
Preferably, the glucose is 3-11g, the sodium chloride is 0.2-1.4g, the monopotassium phosphate is 0.4-1.6g, the zinc sulfate is 0.3-1.1g and the water is 1-1.2L.
Preferably, the tank pressure in the step 1 is controlled to be between 0.03 and 0.05Mpa, and the air flow rate is 2.4m 3/h.
Preferably, the tank pressure in the step 2 is controlled to be between 0.03 and 0.05Mpa, and the air flow rate is 48m 3/h.
Preferably, the air flow rate in the step 3 is 4m3/h, and the tank pressure is kept in the range of 0.02-0.18 MPa.
Preferably, the viable count of each of the bacillus subtilis, the bacillus licheniformis, the rhodopseudomonas palustris and the saccharomyces sphaericus is not less than 100/mL.
Advantageous effects
According to the preparation method of the aquatic product composite microecological preparation prepared by the technical scheme, 4 bacteria of bacillus subtilis, bacillus licheniformis, rhodopseudomonas palustris and saccharomyces sphaeroides are all live bacteria, so that the bacillus subtilis, bacillus licheniformis, rhodopseudomonas palustris and saccharomyces sphaeroides can secrete generated other beneficial factors, such as enzymes, amino acids, biotin, vitamins and the like, and the 4 bacteria and the beneficial factors secreted by the bacteria can decompose substances harmful to cultured animals, serve as plankton and culture materials of the cultured animals, promote the growth of the plankton and the cultured animals, improve the immunity of the cultured animals and prevent diseases.
Detailed Description
The present invention will be further described with reference to the following examples.
Example 1: the preparation method of the aquatic compound microecological preparation comprises the following raw materials: the aquatic product composite micro-ecological preparation comprises bacillus subtilis, bacillus licheniformis, rhodopseudomonas palustris, spherical yeast and a culture solution, wherein the mass ratio of the bacillus subtilis to the bacillus licheniformis to the rhodopseudomonas palustris to the spherical yeast is 2:2:1:1, the viable count of each bacterium of the bacillus subtilis, the bacillus licheniformis to the rhodopseudomonas palustris and the spherical yeast is not less than 100/mL, and the preparation method of the aquatic product composite micro-ecological preparation comprises the following steps:
the culture solution comprises 3g of glucose, 0.2g of sodium chloride, 0.4g of monopotassium phosphate, 0.3g of zinc sulfate and 1L of water.
Step 1: inoculating slant of 4 kinds of bacteria respectively with inoculating loop under aseptic condition to small shake flask containing culture solution, at 27 deg.C, under 0.03Mpa, and at 2.4m air flow rate3H, culturing for 48 hours under the condition of stirring frequency of 46 Hz;
step 2: transferring 4 qualified bacteria cultured in small shake flask into large shake flask containing culture solution under aseptic condition, at culture temperature of 27 deg.C, under 0.03MPa, and with air flow rate of 48m3H, stirring frequency is 35Hz, and culturing is carried out for 72 hours;
and step 3: transferring the inoculum in the large shake flask in the step 2 into a mixing flask under the aseptic operation condition, wherein the temperature is 27 ℃, and the air flow rate is 4m3H, the tank pressure maintaining range is 0.02MPa, the stirring speed is 110r/min, and the culture time is 17 h;
and 4, step 4: and (4) detecting the mixed inoculum obtained in the step (3), and filling the detected inoculum in an aseptic filling room.
Example 2: the preparation method of the aquatic compound microecological preparation comprises the following raw materials: the aquatic product composite micro-ecological preparation comprises bacillus subtilis, bacillus licheniformis, rhodopseudomonas palustris, spherical yeast and a culture solution, wherein the mass ratio of the bacillus subtilis to the bacillus licheniformis to the rhodopseudomonas palustris to the spherical yeast is 2:2:1:1, the viable count of each bacterium of the bacillus subtilis, the bacillus licheniformis to the rhodopseudomonas palustris and the spherical yeast is not less than 100/mL, and the preparation method of the aquatic product composite micro-ecological preparation comprises the following steps:
the culture solution comprises 11g of glucose, 1.4g of sodium chloride, 1.6g of monopotassium phosphate, 1.1g of zinc sulfate and 1.2L of water.
Step 1: inoculating slant of 4 kinds of bacteria respectively with inoculating loop under aseptic condition to small shake flask containing culture solution, at 29 deg.C, under 0.05Mpa, and at 2.4m air flow rate3H, culturing for 48 hours under the condition of stirring frequency of 46 Hz;
step 2: transferring 4 qualified bacteria cultured in small shake flask into large shake flask containing culture solution under aseptic condition, at culture temperature of 29 deg.C, under 0.05Mpa, and air flow rate of 48m3H, stirring frequency is 35Hz, and culturing is carried out for 90 hours;
and step 3: transferring the inoculum in the large shake flask in the step 2 into a mixing flask under the aseptic operation condition, wherein the temperature is 37 ℃, and the air flow rate is 4m3H, the tank pressure maintaining range is 0.18MPa, the stirring speed is 220r/min, and the culture time is 24 h;
and 4, step 4: and (4) detecting the mixed inoculum obtained in the step (3), and filling the detected inoculum in an aseptic filling room.
The technical solutions described above only represent the preferred technical solutions of the present invention, and some possible modifications to some parts of the technical solutions by those skilled in the art all represent the principles of the present invention, and fall within the protection scope of the present invention.
Claims (6)
1. The preparation method of the aquatic product composite microecological preparation is characterized in that the raw materials of the aquatic product composite microecological preparation comprise the following components: the aquatic product composite micro-ecological preparation comprises bacillus subtilis, bacillus licheniformis, rhodopseudomonas palustris, spherical yeast and a culture solution, wherein the mass ratio of the bacillus subtilis to the bacillus licheniformis to the rhodopseudomonas palustris to the spherical yeast is 2:2:1:1, and the preparation method of the aquatic product composite micro-ecological preparation comprises the following steps:
the culture solution comprises glucose, sodium chloride, potassium dihydrogen phosphate, zinc sulfate and water.
Step 1: respectively inoculating inclined planes of 4 bacteria in a small shake flask containing a culture solution by using inoculating loops under the aseptic operation condition, and culturing for 48 hours at the temperature of 27-29 ℃ under the stirring frequency of 46 Hz;
step 2: transferring 4 qualified bacteria cultured in the small shake flask into a large shake flask containing a culture solution under aseptic conditions, and culturing at 27-29 deg.C under stirring frequency of 35Hz for 72-90 hr;
and step 3: transferring the seed bacteria in the large shake flask in the step 2 into a mixing flask under the aseptic operation condition, wherein the temperature is 27-37 ℃, the rotating speed of a stirrer is 110-;
and 4, step 4: and (4) detecting the mixed inoculum obtained in the step (3), and filling the detected inoculum in an aseptic filling room.
2. The method for preparing an aquatic compound microecological preparation according to claim 1, wherein the amount of glucose is 3-11g, the amount of sodium chloride is 0.2-1.4g, the amount of potassium dihydrogen phosphate is 0.4-1.6g, the amount of zinc sulfate is 0.3-1.1g, and the amount of water is 1-1.2L.
3. The method for preparing composite microecological preparation for aquatic products according to claim 1, wherein the tank pressure in step 1 is controlled to 0.03-0.05MPa, and the air flow rate is 2.4m3/h。
4. The method for preparing composite microecological preparation for aquatic products according to claim 3, wherein the tank pressure in step 2 is controlled to 0.03-0.05MPa, and the air flow rate is 48m3/h。
5. The method for preparing aquatic product composite microecological preparation according to claim 1, wherein the air flow rate in step 3 is 4m3H, pot pressure maintenanceThe circumference is 0.02-0.18 MPa.
6. The method for preparing an aquatic composite microecological preparation according to claim 1, wherein the viable count of each of the bacillus subtilis, bacillus licheniformis, rhodopseudomonas palustris and saccharomyces sphaericus is not less than 100/mL.
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| CN202110796918.1A CN113528386A (en) | 2021-07-14 | 2021-07-14 | Preparation method of aquatic product composite microecological preparation |
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| CN202110796918.1A Pending CN113528386A (en) | 2021-07-14 | 2021-07-14 | Preparation method of aquatic product composite microecological preparation |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115184264A (en) * | 2022-06-22 | 2022-10-14 | 广州市致合生物科技有限公司 | Device and method for producing aquatic product microecologics |
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