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CN113166756A - 用于三代测序建库的融合引物、建库方法、测序方法和建库试剂盒 - Google Patents

用于三代测序建库的融合引物、建库方法、测序方法和建库试剂盒 Download PDF

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CN113166756A
CN113166756A CN201980082604.6A CN201980082604A CN113166756A CN 113166756 A CN113166756 A CN 113166756A CN 201980082604 A CN201980082604 A CN 201980082604A CN 113166756 A CN113166756 A CN 113166756A
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sequence
primer
homologous recombination
sequencing
kit
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CN113166756B (zh
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黄标
黄金
丁芬
朱欢文
田志坚
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Wuhan Bgi Medical Laboratory Co ltd
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    • C—CHEMISTRY; METALLURGY
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
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    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms

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Abstract

一种用于三代测序建库的融合引物、建库方法、测序方法和建库试剂盒。融合引物从5’端至3’端依次包括同源重组序列、标签序列和特异性扩增引物序列，其中同源重组序列用于将融合引物的扩增产物进行同源重组拼接，标签序列用于区分不同的扩增产物，特异性扩增引物序列用于与靶标序列结合以进行引物延伸。使用融合引物扩增靶标序列，对扩增产物进行同源重组拼接，增加测序芯片上样本数量，降低测序成本。

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PCT国内申请，说明书已公开。

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  1. PCT国内申请，权利要求书已公开。
CN201980082604.6A 2019-02-13 2019-02-13 用于三代测序建库的融合引物、建库方法、测序方法和建库试剂盒 Active CN113166756B (zh)

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CN114842909A (zh) * 2022-04-08 2022-08-02 武汉明德生物科技股份有限公司 基于三代靶向测序数据的多靶标病原微生物分析方法

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WO2013191400A1 (ko) * 2012-06-19 2013-12-27 (주)지노첵 차세대 염기서열 분석법을 위한 융합 프라이머의 설계방법 그리고 이러한 융합 프라이머 및 차세대 염기서열 분석법을 이용한 표적 유전자의 유전자형 분석방법
CN104293783A (zh) * 2014-09-30 2015-01-21 天津诺禾致源生物信息科技有限公司 适用于扩增子测序文库构建的引物、构建方法、扩增子文库及包含其的试剂盒
CN106754870A (zh) * 2016-11-30 2017-05-31 武汉菲沙基因信息有限公司 一种构建多样品全长转录组混合文库的方法
CN108070643A (zh) * 2017-10-31 2018-05-25 南京格致基因生物科技有限公司 微生物16S rDNA单分子水平测序文库的构建方法

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CN107829146B (zh) * 2017-11-29 2021-07-09 广州赛哲生物科技股份有限公司 一种用于构建16SrRNA基因扩增子测序文库的引物组及构建方法
CN109136222A (zh) * 2018-09-13 2019-01-04 武汉菲沙基因信息有限公司 PacBio测序平台多样品混合测序文库构建的带标签接头及应用

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CN101415838A (zh) * 2003-09-26 2009-04-22 首科安普有限公司 用滚环扩增法扩增多核苷酸
WO2013191400A1 (ko) * 2012-06-19 2013-12-27 (주)지노첵 차세대 염기서열 분석법을 위한 융합 프라이머의 설계방법 그리고 이러한 융합 프라이머 및 차세대 염기서열 분석법을 이용한 표적 유전자의 유전자형 분석방법
CN104293783A (zh) * 2014-09-30 2015-01-21 天津诺禾致源生物信息科技有限公司 适用于扩增子测序文库构建的引物、构建方法、扩增子文库及包含其的试剂盒
CN106754870A (zh) * 2016-11-30 2017-05-31 武汉菲沙基因信息有限公司 一种构建多样品全长转录组混合文库的方法
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Title
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WO2020164015A1 (zh) 2020-08-20

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