CN111999517A - Reagent adding device and method - Google Patents

Abstract

The invention discloses a reagent adding device and method. A reagent adding device for processing slices, comprising: a slide stage, the slide stage defines a accommodating cavity, the accommodating cavity is used for accommodating the slices; a liquid inlet pipe, the liquid inlet pipe is connected with the The accommodating cavity is in communication, and the liquid inlet pipe is used for delivering reagents to the accommodating cavity to submerge the slices; The reagent in the accommodating cavity is discharged. The reagent adding method includes the following steps: placing a plurality of slices in an accommodating cavity of a slide table; delivering reagents into the accommodating cavity and submerging the slices; and discharging the reagents in the accommodating cavity. When using the device and method of the present invention to process slices, the experiment time can be shortened to a certain extent, and the experiment efficiency can be improved.

Description

Reagent adding device and method

technical field

The present invention relates to the biological field, in particular to a reagent adding device and method.

Background technique

In biological experiments, the steps are usually cumbersome. For example, in immunohistochemical experiments, including slide loading, deparaffinization and rehydration, antigen retrieval, blocking, blocking, adding primary antibody, washing, adding secondary antibody, displaying Multiple steps such as coloring, counterstaining, transparency, cover slipping and unloading. In the related art, in each step, a sample adding needle is used to drop corresponding reagents to each slice in turn, which leads to long experiment time and low efficiency.

SUMMARY OF THE INVENTION

The present invention aims to solve at least one of the technical problems existing in the prior art. Therefore, the present invention proposes a reagent adding device, which can shorten the experiment time to a certain extent and improve the experiment efficiency when using the device to process slices.

The invention also provides a reagent adding method, which can shorten the experiment time to a certain extent and improve the experiment efficiency when the slice is processed by the method.

In a first aspect, an embodiment of the present invention provides a reagent adding device for processing slices, including:

a slide stage, the slide stage defines an accommodating cavity, the accommodating cavity is used for accommodating the slice;

a liquid inlet pipe, the liquid inlet pipe is communicated with the accommodating cavity, and the liquid inlet pipe is used to transport reagents to the accommodating cavity to submerge the slice;

and a drain pipe, which is communicated with the accommodating cavity, and is used for discharging the reagent in the accommodating cavity.

The reagent adding device according to the embodiment of the present invention has at least the following beneficial effects: the reagent can flow into the accommodating cavity of the slide stage through the liquid inlet pipe, and the slices on the slide stage can be immersed to participate in the reaction; after the reaction, the reagent can pass through the liquid discharge tube For some universal reagents, using this device can make the reagents reach multiple slices in a short time, and can be quickly discharged in a short time, which can shorten the experimental time and improve the experimental efficiency to a certain extent.

According to the reagent adding device according to other embodiments of the present invention, the slide table includes a carrying platform and a fence, the carrying platform is used to place the slices, the fence surrounds a circumference of the carrying platform, and the carrying platform The platform and the fence define the receiving cavity.

According to the reagent adding device according to other embodiments of the present invention, the liquid inlet pipe and the liquid discharge pipe are respectively located on two sides of the slide table, and extend from the outlet of the liquid inlet pipe to the liquid discharge pipe In the direction of the entrance, the height of the placement surface on the slide table for placing the slices gradually decreases.

The reagent adding device according to other embodiments of the present invention further comprises a liquid inlet pump and a liquid discharge pump, the liquid inlet pump is connected with the liquid inlet pipe, the liquid discharge pump is connected with the liquid discharge pipe, so The liquid inlet pump is used to draw the reagent into the accommodating cavity, and the liquid discharge pump is used to draw the reagent out of the accommodating cavity.

The reagent adding device according to other embodiments of the present invention further includes a sample adding driver and a sample adding member, the sample adding member is connected to the sample adding driver, and the sample adding driver is used to drive the sample adding driver. The sample piece moves, and the sample application piece is used for dripping the reagent onto the slice.

The reagent adding device according to other embodiments of the present invention further includes a first loading member, a second loading member and a loading driving member, wherein the first loading member and the second loading member are all connected to the loading driving member , the first loading member can move toward the slide stage under the driving of the loading driving member to load the slides, and the second loading member can move towards the loading stage under the driving of the loading driving member The slide table is moved to load the coverslip.

According to the reagent adding device according to other embodiments of the present invention, the first loading member includes a first suction cup, the first suction cup is provided with a hollow portion, and the first suction cup is used for sucking the glass slide, so The hollow part is used to avoid the slide area of the slide glass.

The reagent adding device according to other embodiments of the present invention further comprises a cover plate and a cover plate driver, the cover plate is connected to the cover plate driver, and the cover plate can be driven by the cover plate driver Move down to the top of the slide table, and the cover plate is provided with at least two of the following functional parts: a heating part, a glue injection part and a code scanning part.

The reagent adding device according to other embodiments of the present invention further includes a plurality of reagent tanks and catheters, each of the catheters is communicated with one of the reagent tanks, and each of the catheters can be connected to The liquid inlet pipe is communicated.

In the second aspect, an embodiment of the present invention provides a reagent addition method, comprising the following steps:

S10 placing a plurality of slices in the accommodating cavity of the slide table;

S20, delivering reagents into the accommodating cavity and submerging the slices;

S30 discharges the reagent in the accommodating cavity.

The reagent adding method of the embodiment of the present invention has at least the following beneficial effects: the reagent flows into the accommodating cavity of the slide stage, and the slices on the slide stage are immersed to participate in the reaction. After the reaction is completed, the reagent flows out of the accommodating cavity. Using this method can make the reagent reach multiple slices in a short time, and can be quickly discharged in a short time, which can shorten the experimental time and improve the experimental efficiency to a certain extent.

Description of drawings

1 is a schematic diagram of the overall structure of the reagent adding device in the first embodiment;

Fig. 2 is the internal structure schematic diagram of the reagent adding device in Fig. 1;

Fig. 3 is the structural representation of components such as the slide stage and the cover plate in Fig. 1;

4 is a schematic structural diagram of components such as the liquid inlet pipe and the first reagent box in FIG. 1;

FIG. 5 is a flowchart of a reagent addition method.

Reference number:

Slide stage 100, groove 110, first pole 120, liquid inlet pipe 200, outlet 210, liquid conduit 230, liquid discharge pipe 300, first sample loading member 410, second sample loading member 420, first loading member 510, first suction cup 511, second loading member 520, second suction cup 521, slide loading slot 610, cover glass storage slot 620, slide unloading slot 630, cover plate 700, plastic injection member 710, scan code 720 , the second pole 730 , the first reagent tank 810 , the second reagent tank 820 , the waste liquid tank 830 , the first cleaning tank 910 , the second cleaning tank 920 , and the housing 1000 .

Detailed ways

The concept of the present invention and the technical effects produced will be clearly and completely described below with reference to the embodiments, so as to fully understand the purpose, characteristics and effects of the present invention. Obviously, the described embodiments are only a part of the embodiments of the present invention, rather than all the embodiments. Based on the embodiments of the present invention, other embodiments obtained by those skilled in the art without creative efforts are all within the scope of The scope of protection of the present invention.

In the description of the embodiments of the present invention, if the orientation description is involved, for example, the orientation or positional relationship indicated by "up", "down", "front", "rear", "left", "right", etc. is based on the accompanying drawings The orientation or positional relationship shown is only for the convenience of describing the present invention and simplifying the description, rather than indicating or implying that the indicated device or element must have a specific orientation, be constructed and operated in a specific orientation, and therefore should not be construed as a reference to the present invention. limits.

In the description of the embodiments of the present invention, if a feature is referred to as "set", "fixed", "connected", "installed" on another feature, it can be directly set, fixed, or connected on another feature, It can also be indirectly disposed, fixed, connected, mounted on another feature. In the description of the embodiments of the present invention, if "several" is involved, it means more than one; if it involves "plurality", it means more than two; ", should be understood as not including this number, if it involves "above", "below", "within", it should be understood as including this number. If it refers to "first" and "second", it should be understood to be used to distinguish technical features, but not to indicate or imply relative importance, or to imply indicate the number of indicated technical features or to imply indicate the indicated The sequence of technical features.

Referring to FIG. 1 and FIG. 2 , the reagent adding device in this embodiment includes components such as a slide stage 100 , a liquid inlet pipe 200 , and a liquid discharge pipe 300 . There is also a heating wire inside the slide table 100 for use in baking the slices. The slide table 100 defines a receiving cavity into which the slides can be placed. The first reagent box 810 is located below the slide stage 100 and contains reagents. The inlet of the liquid inlet pipe 200 is communicated with the first reagent box 810, and the outlet is communicated with the accommodating cavity. The reagents in the first reagent box 810 can flow into the accommodating cavity through the liquid inlet pipe 200, and submerge the slices to participate in the reaction. The waste liquid tank 830 is located on one side of the slide stage 100 and is used for collecting the waste liquid after the reaction. The remaining reagents after the reaction can flow out of the holding cavity through the drain pipe 300 and flow into the waste liquid tank 830 . In addition, the slide table 100 is provided with a plurality of grooves 110, the size of which is matched with the slide glass, and the slide glass is clamped in the grooves 110 for fixing. However, it should be noted that after the slide table is inserted into the groove 110, its top surface cannot be lower than the top surface of the groove 110, so as to prevent the residual liquid after the reaction from being unable to be drained. The casing 1000 is provided outside each of the above-mentioned components, and protects the above-mentioned components. An exhaust gas sensor may also be provided on the inner wall of the casing 1000 to detect the amount of exhaust gas in the casing 1000, so as to adjust the air volume of the exhaust fan in time.

In this embodiment, the reagent reaches the slide table 100 through the liquid inlet pipe 200 and submerges the slide glass, so that the reagent can reach each slice as soon as possible in a short time. After the reaction is completed, the reagent flows into the waste liquid tank 830 through the drain pipe, The drainage speed is also faster. When the same reagent needs to be added dropwise to all the slides, the device in this embodiment can shorten the reaction time and improve the reaction efficiency compared with the conventional dropwise addition of the sample pen to each slide.

In some embodiments, the slide table 100 includes a carrying platform and a fence, the slices are placed on the carrying platform, the fence is arranged around the circumference of the carrying platform, and the carrying platform and the fence define the above-mentioned accommodating cavity. Two openings may be provided on the fence, which are respectively communicated with the outlet 210 of the liquid inlet pipe 200 and the inlet of the liquid discharge pipe 300 . Alternatively, the outlet of the liquid inlet pipe 200 can also be arranged above the fence. Setting fences can block the reagents to ensure that they can only reach the slide table 100 from the liquid inlet pipe 200 and submerge the slices. A valve can also be set at the inlet of the drain pipe 300, and when the reaction is over, the valve is opened again to prevent some reagents from being discharged before the reaction is over.

Alternatively, in some embodiments, a larger concave portion is directly disposed on the top surface of the slide table 100 without a fence, and the slices are placed in the concave portion, and the concave portion forms the above-mentioned accommodating cavity. Two openings that communicate with the lower concave portion may be provided on the slide table 100 , and the two openings are respectively communicated with the outlet 210 of the liquid inlet pipe 200 and the inlet of the liquid discharge pipe 300 . Alternatively, the outlet of the liquid inlet pipe 200 may be arranged above the lower concave portion.

In some embodiments, a liquid inlet pump and a liquid discharge pump (omitted in the figure) are further provided, the liquid inlet pump is connected with the liquid inlet pipe 200 , and the liquid discharge pump is connected with the liquid discharge pipe 300 . The reagent in the first reagent box 810 is drawn into the liquid inlet pipe 200 by the liquid inlet pump, and flows into the accommodating cavity. The reacted reagent in the containing chamber is drawn into the drain pipe 300 by the drain pump, and flows into the waste liquid tank 830 . Setting up the inlet and outlet pumps can make the inflow and outflow of reagents smoother.

Referring to FIGS. 2 and 3 , in some embodiments, the liquid inlet pipe 200 and the liquid discharge pipe 300 are located on both sides of the slide table 100 respectively, and in the direction from the outlet of the liquid inlet pipe 200 to the inlet of the liquid discharge pipe 300 , the height of the placement surface on the stage 100 for placing the slices (ie, the top surface of the stage 100 ) gradually decreases. In this way, when the reagent flows onto the slide table 100, it can flow toward the direction of the inlet of the drain pipe 300 under the action of gravity, so that the reagent can cover the slides more quickly. After the reaction is completed, the valve of the drain pipe 300 is opened, and after the drain pump is turned on, the reagent can flow into the drain pipe 300 more easily, which is beneficial to quickly discharge the residual reagent. Specifically, the top surface of the slide table 100 is a rectangle, and the outlet of the liquid inlet pipe 200 and the inlet of the liquid discharge pipe 300 can be respectively arranged at both ends of the diagonal of the rectangle. The height of the stage 100 gradually decreases in the direction from the outlet of the liquid pipe 200 to the inlet of the liquid discharge pipe 300 .

It should be noted that, in the above embodiment, since the top surface of the slide table 100 has a certain inclination, when adding reagents, it is necessary to ensure that after the reagents are filled with the slide table 100, at least the slice at the highest position can be submerged to avoid The amount of reagent on the slice near the outlet of the liquid inlet tube 200 is insufficient, and the reaction cannot be performed normally.

In some embodiments, the top surface of the slide table 100 is located within the height range of the inlet of the drain tube 300 , that is, the bottom surface of the inlet of the drain tube 300 is located below the top surface of the slide table 100 , and the top surface of the inlet of the drain tube 300 is located below the top surface of the slide table 100 . The surface is above the top surface of the stage 100 . In this way, after the drain pump is turned on, the reagent can flow into the drain pipe 300 more smoothly.

In some embodiments, a sample application member (for example, the first sample application member 410 and the second sample application member 420) and a sample application driver (omitted from the figure) are further provided, the sample application member is connected to the sample application driver, and the sample application member is connected to the sample application driver. The sample driving part is used to drive the movement of the sample adding part to complete the sample adding. The sample adding part can use a conventional sample adding pen, and the sample adding part can choose a three-axis moving module or a manipulator and other components. A second reagent box 820 is also provided on one side of the slide table 100. The sample adding driver drives the sample adding member to move to the second reagent box 820 to absorb the required reagent, and then moves to the target slice for dripping. When the reagents required for each section are different, you can choose a sample adding piece to add them in sequence. For some more expensive reagents, sample additions can also be used for addition to reduce costs and waste. In this embodiment, if the reagents required for all slices are the same, or the price of the reagents is not high, the reagents can be added through the liquid inlet pipe 200 and the liquid discharge pipe 300; if the reagents required for each slice are different, or the reagents If the price is higher, it can be added by adding sample parts, and the two methods of adding samples can be used together to provide more flexibility.

A cleaning tank (eg, the first cleaning tank 910 and the second cleaning tank 920 ) is also provided on one side of the slide table 100 , and the cleaning tank is filled with cleaning liquid for cleaning the sample application. If the types of reagents that need to be dripped each time are different, it needs to be cleaned before the next dripping to avoid contamination of the reagents.

In some embodiments, a slide loading slot 610 , a cover glass storage slot 620 and a slide unloading slot 630 are further provided on one side of the slide stage 100 , and the slide loading slot 610 contains the slides. The cover glass storage tank 620 contains the cover glass, and the slide glass unloading tank 630 contains the combined body of the slide glass and the cover glass after being sealed. A first loading member 510, a second loading member 520 and a loading driving member (omitted in the figure) are also provided, and the loading driving member can be selected from components such as a three-axis moving module or a manipulator. The first loading member 510 is driven by the loading driving member to take out the slide glass from the slide loading groove 610 and send it to the groove 110 on the slide stage 100; after the reaction is completed, the first loading member 510 is driven by the loading driving member The second loading member 520 takes out the cover glass from the cover glass storage tank 620 and sends it to the glass slide to complete the sealing; after the sealing is completed, the second loading member 520 is driven by the loading driving member to move the groove 110 The finished product is placed in the slide unloading slot 630 . Since the slides are placed on the slides, in order to prevent contamination of the slides, it is necessary to avoid touching the slide area (the area where the slides are placed) when taking them out, while the cover glass does not need to set a specific contact position. For slides and coverslips, use their respective matching loading parts, which can have a better matching degree.

In some embodiments, the first loading member 510 includes a first suction cup 511 , and the second loading member 520 includes a second suction cup 521 , and the slide glass and the cover glass are taken out by suction, which can be handled with care and lowered. Chances of damage to slides and coverslips. The first suction cup 511 is provided with a hollow portion to avoid the slide area of the slide glass, and the second suction cup 521 is not limited. Specifically, the first suction cup 511 can be set in a ring shape, the hollow part in the middle of the ring shape is a hollow part, and the ring part is a position where the slide glass is sucked and fixed. Alternatively, it can also be set to other shapes, such as oval, polygonal or other irregular shapes excavated on the suction cup, which can be selected according to the shape of the slice.

In some embodiments, a cover plate 700 and a cover plate driver (omitted from the figure) are further provided, and the cover plate driver can be selected from components such as a three-axis moving module or a manipulator. The cover plate 700 is provided with at least two of the following functional parts: a heating part, a glue injection part and a code scanning part. For example, the cover plate 700 is provided with a heating element and a plastic injection element 710 (not shown in the figure). A heating wire can be arranged at the bottom of the cover plate 700 to form a heating element, and the cover plate 700 can be driven to move to the top of the slide table 100 by the cover plate driver to heat the reagent. The distance between the cover plate 700 and the slide table 100 The range is 0.1mm-0.3mm. During the movement of the cover plate 700 toward the slide table 100, the glue injection member 710 passes through each row of glass slides in sequence, and injects glue onto the slide glass. After the glue injection, the cover glass is then covered on the slide glass for sealing. piece. In this embodiment, not only heating can be completed through the cover plate 700, but also glue injection can be completed, and the components are integrated to make the structure more compact and occupy less space.

Further, the cover plate 700 is provided with a heating element, a plastic injection element 710 and a code scanning element 720. When the cover plate 700 passes through each row of glass slides in sequence, the code scanning element 720 completes the scanning of the two-dimensional code on each glass slide. In order to facilitate subsequent tracking, the present embodiment further integrates components to make the structure more compact and occupy less space.

In some embodiments, a power supply is further included, a first pole 120 is arranged on the stage 100, a second pole 730 is arranged on the cover plate 700, the first pole 120 and the second pole 730 can be platinum wires, and the two are respectively Electrically connected to the two poles of the power supply. After the cover plate 700 is moved above the wafer stage 100 , and the power is turned on, a working electric field is formed between the wafer stage 100 and the cover plate 700 . The working electric field needs to be a non-uniform electric field, and the field strength in the direction from the cover plate 700 to the stage 100 increases gradually. In a non-uniform electric field, the reagent will produce a dielectrophoresis effect and move in the direction of a strong field, thereby accelerating the reaction with the slice and shortening the reaction time. For example, in immunohistochemical experiments, after the drop of the antibody, the binding of the antibody to the antigen can be accelerated.

Referring to FIGS. 1 and 3 , in some embodiments, there are multiple first reagent boxes 810 and catheters 230 , each first reagent box 810 contains different reagents, and each first reagent box 810 passes through a guide tube 230 . The liquid pipe 230 communicates with the liquid inlet pipe 200 . Each catheter 230 is provided with a valve. When adding a reagent, the valve of the catheter 230 connected to the first reagent box 810 containing the reagent is opened, and the valves of other catheters 230 are closed, and then Turn on the infusion pump to add the required reagents. One of the first reagent boxes 810 contains a cleaning solution. Before adding a different reagent each time, the cleaning solution needs to be added for cleaning, so as not to affect the accuracy of subsequent reactions.

Referring to FIG. 5 , in some embodiments, the method of adding reagents to the slices is as follows: firstly placing a plurality of slices in the holding cavity of the slide table, transporting the reagents into the holding cavity, and submerging the slices for reaction, after the reaction is completed , to discharge the reagent in the holding chamber. The reagent adding method can be implemented by the reagent adding device in each of the above embodiments.

In combination with the above embodiments, when using the device in the embodiment of the present invention to carry out immunohistochemical reaction, slide loading, baking, dewaxing and rehydration, antigen retrieval, blocking, blocking, adding primary antibody, washing, Steps such as adding secondary antibody, developing color, counterstaining, clearing, mounting and unloading slides. During operation, the first loading member 510 is used to place the slide glass with the slice attached on the groove 110 , and if baking the slice is required, the heating wire inside the slide table 100 can be used for baking. After baking the slices, the liquid inlet pipe 200 cooperates with the liquid discharge pipe 300 to transport dewaxing liquid, ethanol (for rehydration), antigen retrieval liquid, blocking agent, blocking liquid and the like to the slide table 100 in sequence. Between each two steps, cleaning fluid needs to be delivered for cleaning. Then, the cover plate 700 is moved to the top of the stage 100 to heat the blocking liquid. After the heating is completed, during the reset process of the cover plate 700, the code scanning component 720 scans the two-dimensional code on each glass slide. Then, the primary antibody is added dropwise through the first sample application member 410. After the dropwise addition, the first sample application member 410 is cleaned in the first cleaning tank 910. At the same time, the cover plate 700 is moved to the top of the slide stage 100 again. Heat to the primary. After the heating is completed, the cleaning liquid is transported to the slide table 100 through the liquid inlet pipe 200 for cleaning, and after the cleaning is completed, the cleaning liquid is discharged from the liquid discharge pipe 300 . Next, the secondary antibody and the color developing agent are added dropwise through the first sample adding member 410, and the process is similar to that of the dropwise addition of the primary antibody. After the color development is completed, the counterstaining solution is added dropwise through the second sample adding member 420 , and after the counterstaining is completed, the second sample adding member 420 is cleaned in the second cleaning tank 920 . A separate sample loading part is set for counterstaining because the counterstaining solution is not easy to clean completely. If the sample loading part is shared with other reagents, the performance of other reagents may be affected. Set a separate cleaning tank for the second sample loading part 420 , in order to prevent the first sample application member 410 from being contaminated by the counterstaining solution during cleaning. After the counterstaining is completed, the transparent liquid is transported to the slide table 100 through the liquid inlet pipe 200. After the transparency corresponds to the time period, the cover plate 700 is moved to the top of the slide stage 100 again. At the same time, the glue injection part 710 drops the mounting medium on a glass slide. The second loading member 520 then places a cover glass on top of the slide to complete the cover slip. After drying the mounting medium, remove the mounted section.

The embodiments of the present invention have been described in detail above in conjunction with the accompanying drawings, but the present invention is not limited to the above-mentioned embodiments, and within the scope of knowledge possessed by those of ordinary skill in the art, various kind of change. Furthermore, the embodiments of the present invention and features in the embodiments may be combined with each other without conflict.

Claims (10)

1. Reagent adds device for handling section, its characterized in that includes:

the slide holder defines a containing cavity, and the containing cavity is used for containing the slices;

the liquid inlet pipe is communicated with the accommodating cavity and is used for conveying a reagent to the accommodating cavity so as to submerge the slices;

and the liquid discharge pipe is communicated with the accommodating cavity and is used for discharging the reagent in the accommodating cavity.

2. The reagent adding device of claim 1, wherein the slide holder comprises a carrying platform and a fence, the carrying platform is used for placing the slide, the fence surrounds the carrying platform, and the carrying platform and the fence define the accommodating cavity.

3. The reagent adding apparatus according to claim 1, wherein the liquid inlet pipe and the liquid outlet pipe are respectively located on both sides of the stage, and a height of a placement surface on the stage on which the slide is placed is gradually reduced in a direction from an outlet of the liquid inlet pipe to an inlet of the liquid outlet pipe.

4. The reagent adding device according to claim 1, further comprising a liquid inlet pump and a liquid outlet pump, wherein the liquid inlet pump is connected to the liquid inlet pipe, the liquid outlet pump is connected to the liquid outlet pipe, the liquid inlet pump is configured to pump the reagent into the accommodating cavity, and the liquid outlet pump is configured to pump the reagent out of the accommodating cavity.

5. The reagent adding device according to claim 1, further comprising a sample adding driving member and a sample adding member, wherein the sample adding member is connected to the sample adding driving member, the sample adding driving member is configured to drive the sample adding member to move, and the sample adding member is configured to drop the reagent onto the slice.

6. The reagent adding device of claim 1, further comprising a first loading member, a second loading member and a loading driving member, wherein the first loading member and the second loading member are both connected with the loading driving member, the first loading member is capable of moving towards the slide stage to load slides under the driving of the loading driving member, and the second loading member is capable of moving towards the slide stage to load coverslips under the driving of the loading driving member.

7. The reagent adding device according to claim 6, wherein the first loading element comprises a first suction cup, a hollow portion is arranged on the first suction cup, the first suction cup is used for sucking the slide, and the hollow portion is used for avoiding a slide area of the slide.

8. The reagent adding device of claim 1, further comprising a cover plate and a cover plate driving member, wherein the cover plate is connected to the cover plate driving member, the cover plate can be driven by the cover plate driving member to move above the slide holder, and at least two of the following functions are provided on the cover plate: heating member, injecting glue piece and sweep the sign indicating number piece.

9. The reagent adding apparatus according to claim 1, further comprising a plurality of reagent tanks and liquid guide tubes, each of the liquid guide tubes being in communication with one of the reagent tanks, and each of the liquid guide tubes being capable of being in communication with the liquid inlet tube.

10. The reagent adding method is characterized by comprising the following steps:

s10, placing a plurality of slices in the accommodating cavity of the slide holder;

s20, conveying the reagent to the accommodating cavity and submerging the slice;

s30 discharging the reagent within the containment cavity.

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