CN111903849A - Feed additive for replacing antibiotics and preparation method thereof - Google Patents
Feed additive for replacing antibiotics and preparation method thereof Download PDFInfo
- Publication number
- CN111903849A CN111903849A CN202010624870.1A CN202010624870A CN111903849A CN 111903849 A CN111903849 A CN 111903849A CN 202010624870 A CN202010624870 A CN 202010624870A CN 111903849 A CN111903849 A CN 111903849A
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- acid
- essential oil
- feed additive
- oil
- chain fatty
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Images
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
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- Life Sciences & Earth Sciences (AREA)
- Polymers & Plastics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Botany (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Physiology (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a feed additive for replacing antibiotics, which comprises plant essential oil, medium-chain fatty acid, organic acid and a carrier; wherein, the medium-chain fatty acid is a fatty acid which can be melted at a higher temperature and forms a solid at normal temperature, thereby forming an envelope of adopted plant essential oil droplets, the carrier is a porous inorganic carrier which can be used in animal feed, and the pores of the carrier can contain the droplets of the plant essential oil; the organic acid comprises benzoic acid and/or fumaric acid. The invention combines the essential oil and medium-chain fatty acid coating by a cold spraying or hot spraying processing technology, improves the stability of the plant essential oil and also improves the palatability of the feed additive. The feed additive can completely replace the conventional feed drug additives of quinocetone, aureomycin and colistin, and can achieve the purpose of inhibiting and killing escherichia coli.
Description
Technical Field
The present invention relates to a formula of feed additive for animal to substitute antibiotic and its preparation method. In particular to a stable and high-efficiency antibiotic-replacing feed additive with strong antibacterial performance, which is prepared by preparing essential oil with antibacterial performance, medium-chain fatty acid and a carrier into compound essential oil through a cold spraying or spraying process, and then mixing and adsorbing the compound essential oil with an organic acid preparation with the antibacterial and antiseptic functions according to a certain proportion.
Background
In 2020, the feed is forbidden to resist the excessive weight and fall to the ground, and the drug feed additive with the growth promoting effect can not be added into the animal feed, so that the development and search of novel, green, safe, efficient and economic antibiotic substitutes with the functions of bacteriostasis and sterilization are in the trend.
The plant essential oil is a secondary metabolite in the plant body and is a substance recognized as safe in the world. Many researches prove that the plant essential oil has various biological activities of resisting bacteria, diminishing inflammation, resisting oxidation, delaying aging and the like, and is widely used in the fields of medical care, food industry, daily chemical products and the like. The cinnamon oil, the fennel oil, the peppermint oil and the like are approved by the rural part of agriculture in China as feed additives which can be added for a long time, and the essential oil products have the characteristics of being natural, free of residues, antibacterial, difficult to generate drug resistance and the like, and can be used as raw materials for replacing antibiotics. The intestinal flora is a complex microecological system consisting of probiotics, conditional pathogenic bacteria and pathogenic bacteria, and the microbial species of the intestinal flora exceed 1000. Wherein the probiotics mainly comprise lactobacillus, bifidobacterium and the like, and the escherichia coli is a common pathogenic bacterium causing diarrhea in the intestinal tract. The medicinal feed additive such as aureomycin, quinocetone, antibiotics, and kitasamycin can kill pathogenic bacteria in intestinal tract, improve feed digestibility, and thus prevent diseases and promote growth. According to the bacteriostasis mechanism of the plant essential oil, the plant essential oil can inhibit any microorganism, but the inhibition strength of the plant essential oil with different active ingredients to the microorganism is different. According to the research of eight plant essential oils on the in-vitro antibacterial effect of common microorganisms in the intestinal tract, such as Wukegang and the like, the eight plant essential oils have certain inhibition and killing capabilities on intestinal pathogenic bacteria and probiotics, wherein cinnamon and oregano have the best comprehensive antibacterial effect. Peppermint oil has strong permeability and can enhance the ability of other essential oil products to pass through cell walls.
Lauric acid, caprylic acid, capric acid, etc. belong to medium-chain fatty acids, and are mainly derived from coconut oil. Research proves that the medium-chain fatty acid is a molecule with antibacterial effect. The bacteriostatic action mechanism is generally considered as follows: 1. directly enter the lipid layer of the bacterial cell membrane to destroy the structure of the cell membrane, so that the content of the bacteria is lost and the transport mechanism is destroyed; 2. the medium-chain fatty acid molecules can easily enter cells to be dissociated, so that H is released, the pH value in the cells is reduced, or the H is transported to the outside of the bacteria by energy consumption, and the bacteria die; 3. inhibiting the production of bacterial lipase, so that bacteria can not attach to intestinal villi; 4. gram positive bacteria are inhibited by uncoupling. A great deal of research of companies such as Provimi and Vitamex Belgium in the Netherlands shows that the addition of the medium-chain fatty acid mixture into the feed can obviously reduce the number of gram-negative pathogenic bacteria in the digestive tracts of pigs and chickens, purify infection of salmonella and the like by long-term feeding, and play a role in reducing stress, reducing the morbidity of mastitis, metritis and agalactia syndrome (MMA) and the like on sows. Dirick et al, in vitro tests simulating the gastric environment of swine, have significant (greater than 10-fold) inhibition of the growth of the bacterial flora in the stomach by complex medium-chain fatty acids (derived from coconut oil, MCTAGL and MCTAG2) at minimum concentrations of 0.35g or 0.025mol per 100g of gastric content, which can be a valuable neurotrophic antibiotic. Clinical trials in vivo have shown that the daily ration containing 2.5% MCTAG2 increased 10% daily and the feed weight ratio decreased 3% compared to the daily ration containing 2.5% soybean oil or 2.5% soybean oil plus 1.5% organic acids, while medium-chain fatty acids reduced the colonies (including total number, e.g., escherichia coli) in the stomach and duodenum by about 10 times as measured after slaughter. Some researches show that the medium-chain fatty acid has the function of changing the intestinal structure of animals, and has the functions of increasing the ratio V/C of the length of villus (V) in the small intestine to the length of crypt (C) in the intestinal wall, thereby increasing the absorption of the villus and shortening the distance of nutrients from the intestinal wall. Meanwhile, the medium-chain fatty acid has the function of adjusting and stabilizing the balance of intestinal microbial flora, and is beneficial to the improvement of the functions of a digestive system, thereby improving the weight gain of livestock and poultry and the feed conversion rate. Research shows that the medium-chain fatty acid can slightly stimulate insulin secretion, improve the glucose content, reduce the decomposition of skeletal muscle protein, increase the absorption of calcium, magnesium and amino acid, promote protein synthesis and have obvious nitrogen-saving effect.
The organic acid preparation, especially benzoic acid, formic acid, fumaric acid and the like, has the important physiological effects of extremely strong bacteriostasis and corrosion prevention, feed palatability improvement, maintenance of gastrointestinal tract health of piglets, feed reward improvement, reduction of piglet nutritional diarrhea and the like. The European Union is the area where antibiotics are forbidden in feed at first, and a whole set of antibiotic-free feed scheme is available at present, wherein benzoic acid and formic acid products are widely applied and are considered as the most effective anti-substitution products.
However, plant essential oils, benzoic acid, formic acid, and medium-chain fatty acids have many disadvantages during use. Cinnamon oil (cinnamaldehyde serving as a main component) is extremely unstable, is easily oxidized into cinnamic acid in the air, and emits a large amount of heat, so that the feed is discolored and deteriorated; meanwhile, cinnamon substances have strong pungent taste at high concentration, the palatability of the feed is seriously influenced, the feed intake is reduced, the production performance of animals is influenced, and the essential oil substances are very easy to be absorbed in the stomach and duodenum of the animals and cannot reach the hindgut segment to play the antibiotic-like role. Benzoic acid, formic acid, caprylic acid, capric acid and lauric acid have strong pungent taste and poor palatability, and the feed intake of the feed is reduced to influence the production performance.
Therefore, it is necessary to find out the essential oil, medium-chain fatty acid and organic acid suitable for the required leading components, the proper adding proportion, the proper preparation method and the proper product form according to the physiological structure of the intestinal tract of animals and the characteristics of various products such as essential oil, medium-chain fatty acid, organic acid, drug feed additive and the like.
Disclosure of Invention
The invention aims to provide a feed additive for replacing antibiotics and a preparation method thereof, and the feed additive can inhibit and sterilize bacteria, improve the stability of plant essential oil and improve the palatability of the feed additive.
In one aspect, to achieve the object of the present invention, the present invention provides a feed additive for replacing antibiotics, which comprises plant essential oil, medium-chain fatty acid, organic acid and a carrier; wherein, the adopted medium-chain fatty acid is fatty acid which can be melted at higher temperature and forms solid at normal temperature so as to form an envelope of plant essential oil droplets, the adopted carrier is a porous inorganic carrier which can be used in animal feed, and the holes of the carrier can contain the plant essential oil droplets, particularly the enveloped plant essential oil droplets; the organic acid used comprises benzoic acid and/or fumaric acid.
In the present invention, medium-chain fatty acids that are meltable at higher temperatures preferably mean medium-chain fatty acids that are meltable at higher temperatures below 100 ℃, for example medium-chain fatty acids that are meltable at 40-90 ℃.
Preferably, in the feed additive of the present invention, the medium-chain fatty acid used is lauric acid. Because the characteristic of low melting point (44 ℃) of lauric acid can be utilized as a coating material, the volatilization of essential oil is prevented, and the pungent smell of plant essential oil, such as the smell of cinnamon oil, is covered.
More preferably, in the feed additive of the present invention, the medium-chain fatty acid further comprises caprylic acid and/or capric acid, wherein the ratio of lauric acid: octanoic acid: the weight ratio of the capric acid can be controlled to be 15: 1: 1 to 3: 1: 1; in the medium-chain fatty acid, if only caprylic acid or capric acid is added in addition to lauric acid, lauric acid: caprylic acid or lauric acid: the weight ratio of the capric acid can be controlled to be 15: 1 to 3: 1.
In the feed additive of the present invention, the porous inorganic carrier used may be silica, such as ultrafine powder of silica, diatomaceous earth, opal powder, etc., or other materials, such as kaolin powder, mica powder, montmorillonite powder, etc.
In the feed additive of the invention, the adopted plant essential oil can be cinnamon oil. More preferably, the plant essential oil further comprises anise oil and/or peppermint oil, wherein the ratio of cinnamon oil: anise oil: the weight ratio of the peppermint oil can be controlled between 160: 2: 1 to 16: 2: 1. In the plant essential oil, if only anise oil is added in addition to cinnamon oil, the cinnamon oil: the weight ratio of the anise oil can be controlled to be 80: 1 to 8: 1; if only peppermint oil is added in addition to cinnamon oil, then cinnamon oil: the weight ratio of the peppermint oil can be controlled between 160: 1 to 16: 1.
In the feed additive of the present invention, the organic acid used may further include formic acid, wherein the ratio of benzoic acid: fumaric acid: the weight ratio of formic acid can be controlled to be 60: 50: 1 to 9: 10: 1. If the organic acids employed in the present invention are benzoic acid and formic acid, the ratio of benzoic acid: the weight ratio of formic acid can be controlled to be 60: 1 to 9: 1; if the organic acids used in the present invention are fumaric acid and formic acid, the ratio of fumaric acid: the weight ratio of formic acid can be controlled between 50: 1 to 10: 1.
The feed additive of the present invention may further comprise other auxiliary ingredients such as an antioxidant, a flavoring agent, etc.
As a specific embodiment of the invention, the content ratio (by weight) of the feed additive replacing antibiotics is as follows:
1-8% of cinnamon oil, 0.1-1% of anise oil, 0.05-0.5% of peppermint oil, 0.05% of antioxidant TBHQ, 5-15% of lauric acid, 1-5% of caprylic acid, 1-5% of capric acid, 1-5% of carrier silicon dioxide, 45-60% of benzoic acid, 40-50% of fumaric acid and 1-5% of formic acid.
In another aspect, to achieve the object of the present invention, the present invention also provides a method for preparing a feed additive for replacing antibiotics, the method comprising the steps of:
(1) adding medium-chain fatty acid weighed according to the formula proportion into a reaction tank, and melting at a higher temperature lower than 100 ℃;
(2) adding the plant essential oil weighed according to the formula proportion into another reaction tank, adding the plant essential oil into the melted material obtained in the step (1), and uniformly mixing;
(3) adding the weighed carrier into the mixture obtained in the step (2), uniformly stirring, and performing cold spraying to obtain the compound essential oil;
(4) and (4) uniformly mixing the compound essential oil obtained in the step (3) and organic acid to obtain a finished product.
Or the method for preparing the feed additive for replacing antibiotics comprises the following steps:
(1) adding medium-chain fatty acid weighed according to the formula proportion into a reaction tank, and melting at a higher temperature lower than 100 ℃;
(2) adding the plant essential oil weighed according to the formula proportion into another reaction tank, adding the plant essential oil into the melted material obtained in the step (1), and uniformly mixing;
(3) spraying the mixture obtained in the step (2) into a weighed carrier while the mixture is hot, uniformly mixing, cooling and sieving to obtain the compound essential oil;
(4) and (4) uniformly mixing the compound essential oil obtained in the step (3) and organic acid to obtain a finished product.
As a specific embodiment of the method of the present invention, the preparation method of the present invention may comprise the steps of:
1) weighing according to the proportion of the formula, mixing lauric acid, caprylic acid and capric acid, putting into a reaction tank with heat preservation, and melting at 75-85 ℃;
2) weighing the cinnamon oil, the fennel oil, the peppermint oil and the antioxidant according to the formula proportion, putting the weighed cinnamon oil, the weighed fennel oil, the weighed peppermint oil and the antioxidant into another reaction tank, adding the melted material 1) and uniformly mixing;
3) weighing silicon dioxide;
4) adding the 3) materials into the 2) tank, uniformly stirring, and carrying out cold spraying operation; or spraying the hot materials in the tank 2) into the materials 3), mixing uniformly, cooling and sieving; obtaining the compound essential oil;
5) mixing the compound essential oil with organic acids such as benzoic acid, fumaric acid, formic acid, etc. to obtain the final product.
The detection of the applicant laboratory and the detection center of microorganisms in Guangdong province proves that the feed additive product replacing antibiotics has the bacteriostatic action similar to antibiotics, and can inhibit and kill Escherichia coli:
A. according to the invention, essential oil and medium-chain fatty acid envelopes are fused by a cold spraying or hot spraying processing technology, so that the stability of the plant essential oil is improved, and the palatability of the feed additive is also improved;
B. clinical tests of the product show that the product can completely replace the traditional antibiotic additive in the feed production and breeding processes, is more suitable for the intestinal structure of animals, improves intestinal microbiota, adjusts the pH value of the intestinal tract, reduces animal diarrhea and improves the production performance;
C. the comprehensive test results show that: the benzoic acid, the formic acid and the essential oil can play a good role in synergism and antibiosis, reduce animal diarrhea, improve the healthy environment of intestinal tracts, reduce the emission of ammonia gas in a pigsty, are safe and environment-friendly, can effectively prevent feed from going moldy and going bad, reduce the use of a mildew inhibitor, and are economical, safe and reliable.
The present invention will be further described with reference to the accompanying drawings and specific embodiments, which are illustrative of certain specific embodiments of the present invention and are not to be construed as limiting the invention. Any changes or modifications on the basis of the present invention are within the protection scope of the present invention.
Drawings
FIG. 1 is a graph comparing the bacteriostatic effect of the feed additive of the present invention;
FIG. 2 is a graph comparing the bacteriostatic effect of the feed additive of the present invention and quinocetone;
FIG. 3 is a comparison of the bacteriostatic effect of the feed additive of the present invention and aureomycin;
FIG. 4 is a graph comparing the bacteriostatic effect of the feed additive of the present invention and the anti-enemy element.
Detailed Description
Preparation examples
The feed additive for replacing antibiotics in the embodiment comprises the following components in percentage by weight:
1-8% of cinnamon oil, 0.1-1% of anise oil, 0.05-0.5% of peppermint oil, 0.05% of antioxidant TBHQ, 5-15% of lauric acid, 1-5% of caprylic acid, 1-5% of capric acid, 1-5% of carrier silicon dioxide, 45-60% of benzoic acid, 40-50% of fumaric acid and 1-5% of formic acid;
the preparation method comprises the following steps:
1) weighing according to the proportion of the formula, mixing lauric acid, caprylic acid and capric acid, putting into a reaction tank with heat preservation, and melting at 75-85 ℃;
2) weighing the cinnamon oil, the fennel oil, the peppermint oil and the antioxidant according to the formula proportion, putting the weighed cinnamon oil, the weighed fennel oil, the weighed peppermint oil and the antioxidant into another reaction tank, adding the melted material 1) and uniformly mixing;
3) weighing silicon dioxide;
4) adding the 3) materials into the 2) tank, uniformly stirring, and carrying out cold spraying operation; or spraying the hot materials in the tank 2) into the materials 3), mixing uniformly, cooling and sieving; obtaining the compound essential oil;
5) mixing the compound essential oil with organic acids such as benzoic acid, fumaric acid, formic acid, etc. to obtain the final product.
Laboratory bacteriostasis test
The feed additive (named as sperm acid guard) sample prepared in the above example is subjected to bacteriostasis comparative test with the conventional medicinal feed additive:
1. the strain is as follows: escherichia coli strain ATCC25922
2. The method comprises the following operation steps:
(1) preparing a culture medium: preparing LB broth and nutrient agar culture medium according to the required amount, boiling on an electronic oven, cooling LB broth to room temperature, accurately transferring 9ml into a test tube, and subpackaging nutrient agar culture medium in triangles.
(2) Physiological saline (0.85%) preparation: 0.85g of sodium chloride is weighed into a 100ml beaker, 100ml of purified water is added, the mixture is dissolved and shaken evenly, and 10ml of normal saline is accurately transferred into a test tube.
(3) Putting 1ml graduated pipette, 10ml graduated pipette, culture dish, culture medium and physiological saline into a sterilization pot, and sterilizing at 121 deg.C for 20min for use.
(4) Preparing a mixed solution containing bacteria and raw materials to be detected: cooling the Escherichia coli strain to room temperature, selecting Escherichia coli in 10ml of sterile physiological saline with inoculating loop on superclean bench, and shaking in vortex mixer to obtain Escherichia coli amount of about 106cfu/ml, transferring 1ml to 9ml of sterile LB broth by using a sterile scale pipette, adding the raw materials to be detected, shaking uniformly by using a mixer to prepare a mixed solution, and placing the mixed solution in a shaking table at 37 ℃ for 200r/min for 0h, 1h, 2h and 3 h.
(5) Transferring 1ml of the mixed solution cultured for 0h, 1h, 2h and 3h into a sterile culture dish by using a sterile scale pipette, pouring 30ml of nutrient agar culture medium, shaking uniformly, condensing, inverting, and simultaneously preparing a nutrient agar culture medium blank and a test control group containing the bacterial liquid and the LB broth. Placing into incubator at 37 deg.C for 24 h.
(6) The growth of colonies was observed in the petri dishes containing 0h, 1h, 2h, 3h and the control group.
3. As a result:
(1) the antibacterial effect of the invention is compared with 0.1%, 0.2%, 0.3% and 0.5% of the antibacterial feed additive
FIG. 1 shows the comparison of the bacteriostatic effects of the feed additive of the present invention; as can be seen from the figure, the growth of the escherichia coli can be completely inhibited only by more than 1h by adding 0.2% of the substitute antibiotic feed additive; the addition of 0.3% of the replacement anti-feed additive completely inhibited the growth of E.coli from the time of contact with E.coli, i.e., 0 h. The anti-colibacillus feed additive is added in an amount of 0.3% to completely inhibit the growth of colibacillus.
(2) Compared with the conventional feed medicament additive, the anti-bacteria feed additive of the invention has the antibacterial effect
The conventional feed pharmaceutical additive for breeding in recent years is mainly quinocetone, aureomycin and enemin, and can achieve the effects of killing animal intestinal harmful bacteria, improving intestinal flora, improving feed digestion and utilization rate, reducing diarrhea and promoting growth according to the law and regulation and suitable addition amount in breeding, wherein the general addition amount is 50ppm of quinocetone, 75ppm of aureomycin and 20ppm of enemin. The three drug feed additives were used in comparative tests with the present alternative feed additive.
A. Comparison with Quinocetone
FIG. 2 shows the comparison of the bacteriostatic effect of the feed additive of the present invention and quinocetone; as can be seen from FIG. 2, the group of quinocetone 50ppm failed to completely inhibit the growth of E.coli, whereas the 0.3% alternative feed additive completely inhibited the growth of E.coli from the beginning.
B. Comparison with aureomycin
FIG. 3 shows the comparison of the bacteriostatic effect of the feed additive of the present invention and aureomycin; as can be seen from FIG. 3, the 75ppm aureomycin group also failed to completely inhibit the growth of Escherichia coli, but a small amount of Escherichia coli was still present, and 0.3% of the anti-feed additive completely inhibited the growth of Escherichia coli from the beginning.
C. Comparison with an antitoxin
FIG. 4 shows the comparison of the bacteriostatic effect of the feed additive of the present invention with that of an anti-enemy agent; as can be seen from FIG. 4, the 20ppm group of colistin-resistant agent completely inhibited the growth of E.coli and from the beginning, the growth of E.coli was inhibited by contact with E.coli, and the 0.3% feed supplement completely inhibited the growth of E.coli from the beginning.
In conclusion, the addition of 0.3% of the alternative anti-feed additive of the invention completely inhibited the growth of E.coli from the beginning when it was brought into contact with it. The experiment shows that the anti-feed additive can completely replace the conventional feed medicament additives of quinocetone, aureomycin and enemin under the experimental condition, and can achieve the purpose of inhibiting and killing escherichia coli.
Examples of clinical trials
The invention replaces the application effect evaluation of the antibiotic feed additive of the sanitary ware and the sourness in the daily ration of the weaned piglets:
test time: 10 months in 2019
Test site: shandong Linyi Junuo county Bainuo farm
The 72 weaned piglets of 24 days old are randomly divided into 3 groups according to male parents, varieties and weights, each group has 3 repetitions, and each repetition has 8 piglets. Three groups were fed with different diets for 15 days.
Control group: feeding basic ration.
Antibiotic group: basal diet + 15% aureomycin (500g/T), 50% quinocetone (100 g/T).
The anti-feed additive group: basic ration plus anti-feed additive (3000 g/T).
The test results are as follows:
the test result shows that: 1) compared with a control group, the antibiotic-substituting feed additive group and the antibiotic group can obviously improve the feed intake and daily gain of piglets, but the antibiotic-substituting feed additive has better performance; 2) compared with a control group, the antibiotic group and the anti-substitution feed additive group can obviously reduce the diarrhea rate of piglets, but the anti-substitution feed additive group is better.
Therefore, the antibiotic replacement feed additive can effectively replace antibiotics, reduce the diarrhea rate of piglets, and improve the feed intake and growth speed of the piglets.
MIC and MBC testing of the feed additive of the invention
The applicant sends the alternative antibiotic feed additive (product name: sourner defensis) to a microbiological analysis detection center in Guangdong province, and effective values appear when the minimum inhibitory concentration MIC and the minimum bactericidal concentration MBC are detected, so that the sourner defensis have the inhibiting and killing effects on escherichia coli, salmonella and staphylococcus aureus causing diarrhea in intestinal tracts, and can well replace antibiotics. Wherein the minimum inhibitory concentrations of the compounds against Escherichia coli8099 (Escherichia coli8099) GDMCC1.180, Salmonella (Salmonella) GDMCC1.163 and Staphylococcus aureus (Staphylococcus aureus) ATCC6358 are respectively 25.00, 25.00 and 12.50mg/ml, and the minimum inhibitory concentrations are respectively 25.00, 25.00 and 25.00 mg/ml.
Claims (10)
1. A feed additive for replacing antibiotics comprises plant essential oil, medium-chain fatty acid, organic acid and carrier; wherein the medium-chain fatty acid is a fatty acid which can be melted at a higher temperature and forms a solid at normal temperature, so that the medium-chain fatty acid can form an envelope of the plant essential oil droplets, the carrier is a porous inorganic carrier which can be used in animal feed, and the plant essential oil droplets can be accommodated in the pores of the carrier; the organic acid comprises benzoic acid and/or fumaric acid.
2. The feed additive of claim 1 wherein the medium chain fatty acid is lauric acid.
3. The feed additive of claim 2, wherein the medium chain fatty acids further comprise caprylic acid and/or capric acid, wherein the ratio of lauric acid: octanoic acid: the weight ratio of the capric acid is 15: 1: 1 to 3: 1: 1.
4. The feed additive of claim 1, wherein the plant essential oil is cinnamon oil.
5. The feed additive of claim 4, wherein the plant essential oil further comprises anise oil and/or peppermint oil, wherein the ratio of cinnamon oil: anise oil: the weight ratio of the peppermint oil is 160: 2: 1 to 16: 2: 1.
6. The feed additive of claim 1 wherein the organic acid further comprises formic acid, wherein the ratio of benzoic acid: fumaric acid: the weight ratio of formic acid is 60: 50: 1 to 9: 10: 1.
7. A feed additive according to claim 1 wherein the medium chain fatty acids are meltable at an elevated temperature below 100 ℃.
8. The feed additive according to any one of claims 1 to 7, wherein the feed additive comprises cinnamon oil 1-8%, anise oil 0.1-1%, peppermint oil 0.05-0.5%, antioxidant TBHQ 0.05%, lauric acid 5-15%, caprylic acid 1-5%, capric acid 1-5%, carrier 1-5%, benzoic acid 45-60%, fumaric acid 40-50% and formic acid 1-5%.
9. A process for preparing a feed additive according to any one of claims 1 to 8, which process comprises the steps of:
(1) adding medium-chain fatty acid weighed according to the formula proportion into a reaction tank, and melting at a higher temperature lower than 100 ℃;
(2) adding the plant essential oil weighed according to the formula proportion into another reaction tank, adding the plant essential oil into the melted material obtained in the step (1), and uniformly mixing;
(3) adding the weighed carrier into the mixture obtained in the step (2), uniformly stirring, and performing cold spraying to obtain the compound essential oil;
(4) and (4) uniformly mixing the compound essential oil obtained in the step (3) and organic acid to obtain a finished product.
10. A process for preparing a feed additive according to any one of claims 1 to 8, which process comprises the steps of:
(1) adding medium-chain fatty acid weighed according to the formula proportion into a reaction tank, and melting at a higher temperature lower than 100 ℃;
(2) adding the plant essential oil weighed according to the formula proportion into another reaction tank, adding the plant essential oil into the melted material obtained in the step (1), and uniformly mixing;
(3) spraying the mixture obtained in the step (2) into a weighed carrier while the mixture is hot, uniformly mixing, cooling and sieving to obtain the compound essential oil;
(4) and (4) uniformly mixing the compound essential oil obtained in the step (3) and organic acid to obtain a finished product.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114601027A (en) * | 2022-04-26 | 2022-06-10 | 驻马店华中正大有限公司 | Acid, essential oil, enzyme and probiotic compound preparation and preparation method thereof |
| CN115349577A (en) * | 2022-08-22 | 2022-11-18 | 华中农业大学 | Feed additive containing organic acid and medium-chain fatty acid and application thereof |
| CN115736108A (en) * | 2022-11-02 | 2023-03-07 | 江苏奥迈生物科技有限公司 | Composite medium-chain fatty acid feed additive and preparation method and application thereof |
| CN116098240A (en) * | 2022-12-02 | 2023-05-12 | 安佑生物科技集团股份有限公司 | Feed additive for improving pig's ability to resist enveloped virus and preparation method thereof |
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| CN107950792A (en) * | 2017-12-12 | 2018-04-24 | 生物源生物技术（深圳）股份有限公司 | A kind of New Type Complex Fead Additives and its preparation method and application |
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| CN106942472A (en) * | 2016-11-14 | 2017-07-14 | 江苏隆达生物科技有限公司 | A kind of feed addictive containing the fragrant phenol plants essential oil of coating wild marjoram and production method |
| CN107950792A (en) * | 2017-12-12 | 2018-04-24 | 生物源生物技术（深圳）股份有限公司 | A kind of New Type Complex Fead Additives and its preparation method and application |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114601027A (en) * | 2022-04-26 | 2022-06-10 | 驻马店华中正大有限公司 | Acid, essential oil, enzyme and probiotic compound preparation and preparation method thereof |
| CN115349577A (en) * | 2022-08-22 | 2022-11-18 | 华中农业大学 | Feed additive containing organic acid and medium-chain fatty acid and application thereof |
| CN115736108A (en) * | 2022-11-02 | 2023-03-07 | 江苏奥迈生物科技有限公司 | Composite medium-chain fatty acid feed additive and preparation method and application thereof |
| CN116098240A (en) * | 2022-12-02 | 2023-05-12 | 安佑生物科技集团股份有限公司 | Feed additive for improving pig's ability to resist enveloped virus and preparation method thereof |
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