CN111869779A - Grifola frondosa active polysaccharide composite tablet candy as well as preparation method and application thereof - Google Patents

Abstract

The invention relates to a tableted candy, in particular to a composite tableted candy of Grifola frondosa active polysaccharide and a preparation method and application thereof, comprising the components in parts by weight: 40-70 parts of Grifola frondosa active polysaccharide extract , 10-30 parts of bitter gourd extract, 10-30 parts of isomalt, 5-20 parts of microcrystalline cellulose, 0-5 parts of silicon dioxide, 0-5 parts of magnesium stearate, 0.5 parts of film coating agent -3 copies. Beneficial effects of the present invention: The present invention uses Grifola frondosa and bitter melon as main raw materials, and the active ingredients of the tablet candy prepared by the compatibility of the two are high, and they assist each other. Significant hypoglycemic effect. The addition of isomalt in the present invention not only helps to control blood sugar, but also further regulates the mouthfeel of the tableted candy, and the prepared tableted candy has a mellow mouthfeel and is easy to be accepted by consumers; the product dosage form of the present invention is a tableted candy , which is convenient for consumers to carry and eat.

Description

A kind of Grifola frondosa active polysaccharide composite tableting candy and preparation method and use

technical field

The invention relates to a tableted candy, in particular to a composite tableted candy of Grifola frondosa active polysaccharide and a preparation method and application thereof.

Background technique

Diabetes is a chronic metabolic disease characterized by hyperglycemia. It occurs because the body cannot form or effectively use insulin, resulting in absolute or relative insulin deficiency. The symptoms are weight loss, body weight loss, and fatigue. Lack of energy. The more severe the diabetes, the more significant the body weight loss; the longer the course of the disease, the continuous increase in blood sugar, which is called "diabetes syndrome" in traditional Chinese medicine. Long-term untreated or improper treatment may lead to chronic complications of diabetes, including diabetic nephropathy, diabetic cardiovascular disease, etc. These chronic complications are an important cause of diabetes death and disability. At present, diabetes has become one of the global public health problems. The eighth edition of the Global Diabetes Map released by the International Diabetes Federation (IDF) in 2017 shows that there are currently 425 million diabetic patients in the world, and it is estimated that by 2045, there will be nearly 7 million people with diabetes. With the continuous advancement of the aging process in our country, coupled with the continuous change of public life concepts, the incidence of diabetes continues to increase. China is the hardest hit area for diabetes. IDF statistics show that the number of people with diabetes in China ranks first in the world, reaching 114 million. In stark contrast to the huge diabetic population, the current situation of diabetes treatment in my country is worrying. Diabetes awareness rate is low, treatment rate is low, and complication rate is high.

Diabetes is mainly divided into two categories, including type I diabetes and type II diabetes. Type I diabetes mellitus, also known as insulin-dependent diabetes mellitus, is a type of disease in which the body produces abnormal autoimmune responses that cause damage to islet β cells and reduce insulin secretion. The proportion of patients with type 1 diabetes in the overall diabetic population in my country is about 5%. Type 2 diabetes, also known as non-insulin-dependent diabetes mellitus, is a type of disease with great heterogeneity that changes from the main manifestation of insulin resistance with relative insulin insufficiency to the main manifestation of insulin insufficiency and insulin resistance. Totally clear. Type 2 diabetes accounts for about 95% of the total diabetes population and is the most common type of diabetes in clinical practice. Currently, there are more than 500 million type 2 diabetes patients in the world, and China accounts for about 1/3 of the patients. In addition, there is a group of pre-diabetic populations, that is, those who have elevated fasting plasma glucose or (and) 2h oral glucose tolerance test plasma glucose but do not meet the diagnostic criteria for diabetes. National survey data in 2010 showed that the prevalence of diabetes in adults was 11.6%, and 50.1% of adults were in pre-diabetes, that is, more than 700 million people need effective intervention on blood sugar.

Although insulin injections and oral drugs have achieved obvious therapeutic effects on diabetes, different side effects have also occurred, especially damage to large blood vessels, retina, and nerve tissue. Epidemiological surveys have shown that various dietary patterns have a good preventive effect on the occurrence of type 2 diabetes, which means that nutritional regulation and dietary treatment are effective in preventing the occurrence of diabetes and its complications. Therefore, for those who have been diagnosed with diabetes, hypoglycemic drugs are the first choice, and at the same time, they can be supplemented with health food or functional food that is safe, has no side effects, and has clear efficacy to assist in lowering blood sugar. For people with pre-diabetes, the "China Guidelines for the Prevention and Treatment of Type 2 Diabetes" recommends that pre-diabetic patients should reduce the risk of diabetes through diet control and exercise, and drug intervention is not recommended to prevent diabetes. Pre-diabetes is a reversible process, and early intervention for pre-diabetic people can reduce the incidence of type 2 diabetes. Therefore, eating health food or functional food with hypoglycemic effect can be the first choice for such people to prevent diabetes.

At present, most of the supplementary hypoglycemic health foods and functional foods sold in the Chinese market are mainly based on Chinese herbal medicines containing flavonoids and saponins and chromium elements, that is, they are still in the first generation (general nutritional products) and the second generation ( However, Japan, Europe and the United States and other countries have developed into the third generation of new functional foods extracted from natural substances, which will dominate the market in the future. Therefore, the development of safe hypoglycemic functional foods with no side effects and clear efficacy, and the use of natural products with hypoglycemic activity as supplementary foods and adjuvant treatments for diabetic patients have attracted increasing attention, and are also future research directions.

In addition, most of the currently commercially available hypoglycemic functional foods are mainly granules or tea bags, which are inconvenient to take and have a poor taste, which easily reduces the enthusiasm of people with diabetes to take them.

SUMMARY OF THE INVENTION

In view of the above deficiencies, the present invention provides a compound tableting candy with Grifola frondosa active polysaccharide. The tableting candy uses common food Grifola frondosa and bitter gourd as main raw materials, and has been verified by an alloxan-induced hyperglycemia rat model. Hypoglycemic function, easy to eat, safe without side effects, good taste, suitable for long-term use by diabetics.

The present invention achieves the above object through the following schemes:

In the first aspect, the present invention provides a Grifola frondosa active polysaccharide composite tablet candy, comprising the following components in parts by weight: 40-70 parts of Grifola frondosa active polysaccharide extract, 10-30 parts of bitter gourd extract, 10-30 parts of isomalt, 5-20 parts of microcrystalline cellulose, 0-5 parts of silicon dioxide, 0-5 parts of magnesium stearate, and 0.5-3 parts of film coating agent.

Preferably, the preparation method of the Grifola frondosa polysaccharide extract is obtained by water extraction, concentration, centrifugation, microfiltration, ultrafiltration and spray drying of the Grifola frondosa raw material.

Preferably, the preparation method of the bitter melon extract is obtained by water extraction, concentration and spray drying of the dry raw materials of bitter melon.

Grifola frondosa, also known as polypore, chestnut mushroom, cloud mushroom, lotus mushroom, etc., belongs to Basidiomycetes, Polypore, Polyporeaceae, Polypore. The fruit body of Grifola frondosa has soft flesh, crisp like magnolia, taste like chicken shreds, delicious taste, unique fragrance, and rich nutrition. It is shown that the polysaccharides of Grifola frondosa extracted from its fruiting bodies or mycelium have significant anti-tumor, hypoglycemic and immune system functions.

Bitter melon is a traditional Chinese medicinal and edible plant. It is rich in various active ingredients such as balsam pear polysaccharides, saponins, polypeptides, flavonoids, etc. health function.

Isomalt is a carbohydrate derived from sucrose. Isomalt has the characteristics of low calorie and low GI value, and will not be digested by saliva, gastric acid and pancreatic juice after ingestion, and will not be hydrolyzed into glucose and fructose until the small intestine is absorbed. Abdominal sensation, non-acidity in the mouth, digestive permeability, production of short-chain fatty acids (especially butyrate) and probiotics (especially bifidobacteria), etc., are suitable for people with diabetes and obesity who need to control blood sugar and calories Very beneficial, isomalt has a mild taste and also acts as a functional sweetener to sweeten foods.

In particular, the present invention makes the tableted candy of the present invention have a targeted hypoglycemic effect through the combination of the above-mentioned components and the compounding effect between the components, which will not lead to hypoglycemia caused by excessive or wrong taking, and also It is safe and effective to lower the blood sugar of hyperglycemic people or people with diabetes in a targeted manner.

In the second aspect, the preparation method of the above-mentioned Grifola frondosa active polysaccharide composite tableting candy is provided, comprising the following steps:

(1) Weighing: Weigh the raw material components according to the dosage;

(2) Sieve: pass the weighed material through a 40-60 mesh screen;

(3) Mixing: mixing the sieved raw materials for 10-20min;

Optionally (4) granulation: the mixed material is granulated to make uniform granules;

(5) Tablet compression: put the mixed material or the granulated material into the hopper of the tableting machine for tableting;

(6) coating: the compressed confectionery tablet extruded is coated;

Preferably, the granulation includes dry granulation or wet granulation.

In a third aspect, the composite tablet candy prepared by the above preparation method is provided.

The fourth aspect provides the use of the above-mentioned Grifola frondosa active polysaccharide composite tablet candy, including lowering blood sugar, especially reducing the blood sugar of diabetic patients or people with high blood sugar.

Compared with the existing invention, the beneficial effects of the present invention are:

(1) the present invention adopts Grifola frondosa and balsam pear as the main raw materials, the Grifola frondosa is extracted and the ultrafiltration membrane separation technology is used to intercept and enrich the Grifola frondosa active polysaccharide to obtain the Grifola frondosa polysaccharide extract, and the bitter melon is extracted with water to obtain a The balsam pear saponin with a clear hypoglycemic effect was used as a landmark component to guide the preparation, and the balsam pear saponin-rich balsam pear saponin extract was prepared. The tablet candy prepared by the combination of the two has a high content of active ingredients and is mutually supportive, and has a significant hypoglycemic effect as evaluated by an alloxan-induced hyperglycemia rat animal model.

(2) the present invention adds isomalt, which not only helps to control blood sugar but also further regulates the mouthfeel of the tablet candy, and the prepared tablet candy has a mellow mouthfeel and is easy to be accepted by consumers;

(3) The product dosage form of the present invention is a tablet candy, which is convenient for consumers to carry and eat.

Detailed ways

The present invention will be further described below in conjunction with specific embodiments.

Example 1:

A Grifola frondosa active polysaccharide composite tableting candy is composed of the following components in a ratio of parts by weight: 40 parts of Grifola frondosa polysaccharide extract, 20 parts of bitter gourd extract, 21 parts of isomalt, microcrystalline 15 parts of cellulose, 2 parts of silicon dioxide, 2 parts of magnesium stearate, 2 parts of film coating agent (hydroxypropyl methylcellulose and pullulan).

Wherein, the preparation method of the Grifola frondosa polysaccharide extract is obtained by water extraction, concentration, centrifugation, microfiltration, ultrafiltration and spray drying of Grifola frondosa raw materials.

Wherein, the preparation method of the bitter gourd extract is obtained by water extraction, concentration and spray drying of dry raw materials of bitter gourd. .

The preparation method of above-mentioned Grifola frondosa active polysaccharide composite tableting candy, the method comprises:

Weighing → sieving → mixing → tableting → coating → packaging

A. Weighing: According to the formula, weigh the raw and auxiliary materials according to the dosage;

B. Sieve: Pass the weighed material through a 40-60 mesh sieve;

C. Mixing: Pour the sieved raw and auxiliary materials into the three-dimensional mixing tank and mix for 10-20min;

D. Tablet compression: take out the evenly mixed raw and auxiliary materials and put them into the hopper of the tableting machine for tableting, the tablet weight is 0.5g/tablet;

E, coating: the pressed confectionery tablet is coated in the coating pan;

F. Packaging: After drying and cooling the coated tablets, pack them according to the packaging requirements to obtain the finished product.

Example 2:

A Grifola frondosa active polysaccharide composite tableting candy is composed of the following materials according to the ratio of parts by weight: 50 parts of Grifola frondosa polysaccharide extract, 20 parts of bitter gourd extract, 15 parts of isomalt, microcrystalline fiber 10 parts of vegetarian, 3 parts of magnesium stearate, 2 parts of film coating agent (hydroxypropyl methylcellulose and pullulan).

Wherein, the preparation method of the Grifola frondosa polysaccharide extract is obtained by water extraction, concentration, centrifugation, microfiltration, ultrafiltration and spray drying of Grifola frondosa raw materials.

Wherein, the preparation method of the bitter gourd extract is obtained by water extraction, concentration and spray drying of dry raw materials of bitter gourd.

The preparation method of above-mentioned Grifola frondosa active polysaccharide tableting candy, the method comprises:

Weighing → sieving → mixing → granulation → tableting → coating → packaging

A. Weighing: According to the formula, weigh the raw and auxiliary materials according to the dosage;

B. Sieve: Pass the weighed material through a 40-60 mesh sieve;

C. Mixing: Pour the sieved raw and auxiliary materials into the three-dimensional mixing tank and mix for 10-20min;

D. Granulation: the mixed material is dry granulated to make uniform granules;

E. Tabletting: put the granulated material into the hopper of the tablet press for tableting, and the tablet weight is 0.5g/tablet;

F. Coating: the pressed confectionery tablets are coated in a coating pan;

G. Packaging: After drying and cooling the coated tablet, pack it according to the packaging requirements to obtain the finished product.

Test Example 1:

Using the Grifola frondosa active polysaccharide tablet candy in Example 1 as a sample, animal experiments were conducted to evaluate the hypoglycemic effect of the present invention.

1. Method:

SPF grade SD rats, body weight 130-150g, provided by Guangdong Medical Laboratory Animal Center, production license number: SCXK (Guangdong) 2013-0002, relative humidity (50±5)%, room temperature (22±2) °C, with free access to food and water.

Maintenance feed: formula of common feed; high-calorie feed: maintenance feed for rats and mice 52.6%, sucrose 15.0%, egg yolk powder 15.0%, lard 10.0%, casein 5.0%, cholesterol 1.2%, calcium bicarbonate 0.6%, stone powder 0.4% %, sodium cholate 0.2%.

The normal maintenance diet was adapted to feeding for 7 days, and 8 rats were randomly selected as the normal control group. The rats were fasted for 24 hours (drinking water freely), except for the normal control group, the other rats were intraperitoneally injected with alloxan at 105 mg/kg. After the injection, the normal control group was given ordinary feed, and the model group was given high-fat feed. After 5-7 days, the fasting blood glucose was detected, and the model rats with the blood glucose value of 10-25 mmol/L were selected to continue the experiment. The rats were randomly divided into 5 groups with 8 rats in each group: model control group, metformin group, low-dose group of Grifola frondosa active polysaccharide tablet candy, middle-dose group of Grifola frondosa active polysaccharide tablet candy, and Grifola frondosa active polysaccharide tablet candy group. Tablet candy high-dose group.

At the same time, 20 SD rats were taken, fasted for 3-5 hours, blood was taken from the tail vein to measure the blood glucose level using a blood glucose meter, and they were randomly divided into the blank control group and the Grifola frondosa active polysaccharide compound tablet candy group and the high-dose group. 10 per group.

Each group was given the corresponding test substance by gavage, once a day, after 30 consecutive days, the fasting blood glucose was detected. The specific experimental groups and dosing schedules are shown in Table 1.

Table 1 Experimental grouping and dosing schedule

2. Results and Analysis

(1) Effects of Grifola frondosa active polysaccharide tablet candy on fasting blood sugar in normal rats

Table 2 shows the experimental results of the effect of samples on fasting blood glucose in normal rats.

The effect of table 2 samples on fasting blood glucose in normal rats (X±S) (n=10)

Compared with the blank control group, the Grifola frondosa active polysaccharide compound tablet candy group had no significant difference in fasting blood glucose values before the experiment and at the end of the experiment, and there was no significant difference with the normal control group. This shows that the Grifola frondosa active polysaccharide compound tablet candy has no effect on the fasting blood sugar of normal rats, and will not cause hypoglycemia.

(2) Effects of Grifola frondosa active polysaccharide tablet candy on fasting blood sugar in model animals

The experimental results are shown in Table 3.

The influence of table 3 samples on fasting blood glucose of diabetic rats (n=8)

group Fasting blood glucose (mmol/L) normal control group 6.25±0.24 model control group 11.02±6.21^△△ positive control group 9.71±3.10^△ Grifola frondosa active polysaccharide compound tablet candy-low dose group 8.80±1.78 Grifola frondosa active polysaccharide compound tablet candy-medium dose group 7.85±0.85* Grifola frondosa active polysaccharide compound tablet candy-high dose group 7.75±1.03*

Note: Compared with the normal control group, △p<0.05, △△p<0.01; compared with the model control group, *p<0.05, **p<0.01.

The results showed that the fasting blood glucose value of the rats after modeling was significantly higher than that of the normal control group. After 30 days of administration, the blood glucose value of each administration group decreased to varying degrees. Compared with the model control group, the fasting blood glucose value of the group decreased significantly, and the difference between the groups was statistically significant. Moreover, the effect of Grifola frondosa active polysaccharide compound tableting candy in low, medium and high dose groups was better than that of the positive control, which indicated that the Grifola frondosa active polysaccharide compound tableting candy had a significant hypoglycemic effect on hyperglycemic or diabetic populations.

On this basis, the inventors also tested the synergistic effect of the combination of Grifola frondosa polysaccharide extract and bitter melon extract in hypoglycemic and other aspects, and the test results confirmed that the synergistic effect of the combination was significant.

The above are only preferred specific embodiments of the present invention, but the protection scope of the present invention is not limited thereto. The equivalent replacement or change of the solution and its concept shall be included within the protection scope of the present invention.

Claims (8)

1. A Grifola frondosa active polysaccharide composite tableting candy is characterized in that, comprises the following components in parts by weight: 10-30 parts of maltitol, 5-20 parts of microcrystalline cellulose, 0-5 parts of silicon dioxide, 0-5 parts of magnesium stearate, and 0.5-3 parts of film coating agent. 2. a kind of Grifola frondosa active polysaccharide composite tableting candy according to claim 1, is characterized in that, the preparation method of described Grifola frondosa polysaccharide extract is that Grifola frondosa raw material is extracted by water, concentrated, centrifuged, slightly Filtration, ultrafiltration and spray drying. 3. A kind of Grifola frondosa active polysaccharide composite tableting candy according to claim 1, is characterized in that, the preparation method of described bitter gourd extract is that bitter gourd dried raw material is obtained through water extraction, concentration and spray drying. 4. a method for preparing the Grifola frondosa active polysaccharide composite tableting candy described in any one of claims 1-3, is characterized in that, comprises the following steps: (1) Weighing: Weigh the raw material components according to the dosage; (2) Sieve: pass the weighed material through a 40-60 mesh screen; (3) Mixing: mixing the sieved raw materials for 10-20min; Optionally (4) granulation: the mixed material is granulated to make uniform granules; (5) Tablet compression: put the mixed material or the granulated material into the hopper of the tableting machine for tableting; (6) Coating: The extruded compressed confectionery tablet is coated. 5. The preparation method according to claim 4, wherein the granulation comprises dry granulation or wet granulation. 6. The composite tablet candy prepared by the preparation method according to claim 4 or 5. 7. The use of the Grifola frondosa active polysaccharide composite tablet candy according to any one of claims 1-3 or claim 6, characterized in that it includes hypoglycemic. 8. The use according to claim 7, characterized in that it is used to lower blood sugar in diabetic patients or people with hyperglycemia.