CN111838168A

CN111838168A - Bactericidal composition

Info

Publication number: CN111838168A
Application number: CN201910366013.3A
Authority: CN
Inventors: 罗昌炎; 詹姆斯.T.布里斯托
Original assignee: Jiangsu Rotam Chemical Co Ltd
Current assignee: Jiangsu Rotam Chemical Co Ltd
Priority date: 2019-04-30
Filing date: 2019-04-30
Publication date: 2020-10-30

Abstract

The invention provides a bactericidal composition which contains active ingredients of isopyrazam and metiram, wherein the weight percentage of the isopyrazam to the metiram is 10:1-1: 50. The invention also relates to the application of the bactericidal composition in controlling harmful fungi and bacteria on cereals, vegetables, fruits, ornamental plants and grapevines. The invention also relates to the use of the fungicidal compositions for controlling pathogenic or saprophytic fungi and bacteria in soils or cultivation media, by application to the locus where control is desired. The invention also relates to the use of said fungicidal compositions for the treatment of seeds in order to protect them from attack by phytopathogens carried.

Description

Bactericidal composition

Technical Field

The present invention relates to a germicidal composition; the invention also relates to application of the bactericidal composition in preventing or preventing plant pathogenic fungi from infecting plants in the agricultural or horticultural field.

Background

At present, for disease control which is easy to generate resistance in agriculture, the pesticide varieties with different action mechanisms are optimally mixed, and if the mixture ratio is reasonable, the obvious synergistic effect can be generated, so that the field control effect is obviously better than the effect of each single agent. The bactericide containing a single active ingredient has certain defects in agricultural disease control, not only is pathogenic bacteria easy to generate drug resistance, but also is easy to cause pollution to food and environment after being continuously used for multiple times, and the defects can be overcome by reasonably mixing the active ingredients of the bactericide. The reasonable compounding ensures that the effective components generate synergistic action, can improve the control effect, reduce the using amount of the effective components, save the cost, delay the generation of drug resistance of pathogenic bacteria, and further can lighten or even avoid the pollution of pesticides to food and environment.

With regard to the activity of pesticides, in particular with regard to crop protection, one of the core problems of the research carried out in this technical field is the improvement of the properties, in particular in terms of biological activity, and the maintenance of this activity over a certain period of time.

Isopyrazam, chemical name 3-difluoromethyl-1-methyl-1H-pyrazole-4-carboxylic acid (9-isopropyl-1, 2,3, 4-tetrahydro-1, 4-methylene-naphthalen-5-yl) amide. Pyrazolonaphthalein compounds are dehydropythium succinate inhibitor fungicides developed by shindak corporation. Isopyrazam has been disclosed in CN 100448876C. The isopyrazam has higher inhibition activity on the growth of hyphae of cucumber brown spot fungus, cucumber anthracnose pathogen, pepper anthracnose pathogen, tomato early blight pathogen, apple spot pathogen, pepper phytophthora capsici, potato late blight pathogen, tomato gray mold pathogen, tomato sclerotinia sclerotiorum, rice blast pathogen and apple ring rot pathogen.

Since the environmental and economic requirements for fungicides are now constantly increasing, for example with regard to the spectrum of activity, toxicity, selectivity, application rate, residue composition and advantageous production feasibility, and also since problems can occur, for example, with regard to resistance, the development of new fungicides which are superior in some respects to existing fungicides is a constant task.

Disclosure of Invention

The invention aims to provide a bactericidal composition, which has a gain effect on the prevention and treatment effect by combining isopyrazam and metiram.

The long-term single application of an active compound to prevent and treat diseases can cause the generation of the drug resistance of the diseases, and the prevention effect of the compound is reduced or even completely lost. In order to reduce the risk of resistance of phytopathogens, the application of synergistic compositions for controlling harmful phytopathogenic fungi is one of the methods commonly used at present.

It has now surprisingly been found that the simultaneous, i.e. combined or separate, application of isopyrazam and metiram, or the sequential application of isopyrazam and metiram, leads to better control of phytopathogenic fungi than the application of the individual compounds alone.

The combination of isopyrazam and metiram not only further increases the spectrum of action on the phytopathogens which are normally expected to be controlled, but also achieves a synergistic effect.

The invention provides a bactericidal composition, which combines the isopyrazam and the metiram, so that the obtained composition has a gain effect on the prevention and treatment effect, widens the bactericidal spectrum, plays a role in multiple purposes, and effectively slows down or avoids the generation of drug resistance of pathogenic bacteria. Surprisingly, the fungicidal activity of the fungicidal compositions according to the invention is significantly higher than the sum of the activities of the individual active compounds. In other words, there is an unpredictable, truly present synergistic effect, not just a supplementation of activity.

The invention discloses a sterilization composition, which is realized by adopting the following technical scheme:

a germicidal composition, characterized by: the bactericidal composition contains active ingredients of isopyrazam and metiram, wherein the weight percentage of the isopyrazam to the metiram is 10:1-1:50, more preferably 5:1-1:40, still more preferably 1:1-1:30, more preferably 1:1-1:25, more preferably 1:1-1:20, more preferably 1:1-1:15, more preferably 1:5-1:12, and more preferably 1:8-1: 12.

In the invention, the weight percentage of the isopyrazam to the metiram can be 10:1,9:1,8:1,7:1,6:1,5:1,4:1,3:1,2:1,1:1,1:2,1:3,1:4,1:5,1:6,1:7,1:8,1:9,1:10,1:11,1:12,1:13, 1: 14,1:15,1:16,1:17,1:18,1:19,1:20,1:21,1:22,1:23,1:24,1:25,1:26,1:27,1:28,1:29,1:30,1:31, 1: 32,1:33,1:34,1:35,1:36,1:37,1:38,1:39,1:40,1:41, 43,1: 42,1: 44,1: 46,1: 48,1:49, 1:50.

The isopyrazam represents an epimeric mixture of the racemic compound of formula Ia (cis) and the racemic compound of formula Ib (trans), the ratio of the racemic compound of formula Ia (cis) to the racemic compound of formula Ib (trans) being from 1000:1 to 1: 1000.

Preferably, the isopyrazam represents an epimeric mixture of the racemic compound of the formula Ia (cis) and the racemic compound of the formula Ib (trans), wherein the content of the racemic compound of the formula Ia (cis) is 80 to 99% by weight, preferably 85 to 90% by weight.

Preferably, the isopyrazam represents an epimeric mixture of the racemic compound of the formula Ia (cis) and the racemic compound of the formula Ib (trans), wherein the content of the racemic compound of the formula Ib (trans) is 60 to 99% by weight, preferably 64 to 70% by weight.

A sterilization composition comprises active ingredients of isopyrazam and metiram, and also comprises a filling agent and/or a surfactant.

The bactericidal composition is in the dosage form of aerosol, capsule suspension, cold atomization concentrate, hot atomization concentrate, capsule granule, fine granule, ready-to-use solution, sprayable powder, emulsifiable concentrate, oil-in-water emulsion, water-in-oil emulsion, large granule, micro granule, oil dispersible powder, oil miscible flowable concentrate, oil miscible liquid, foam, paste, suspension concentrate, soluble concentrate, suspension, seed coating agent, wettable powder, water dispersible granule, soluble powder, micro-capsule suspension, coated granule, extruded granule, missible oil, microemulsion, aqueous emulsion, effervescent tablet, ultra-low volume liquid, suspension emulsion, ultra-low volume cold atomization preparation, ultra-low volume hot atomization preparation, twin pack (twin pack), seed treatment dry powder, seed treatment emulsion, seed treatment suspension, micro-emulsion, Seed treatment liquid, seed treatment dispersible powder, seed treatment microcapsule suspending agent, seed treatment gel, suspoemulsion and emulsion granules.

The bactericidal composition comprises 5% -90% of the isopyrazam and the metiram active ingredients, preferably 10% -80%, and more preferably 20% -70%.

In the bactericidal composition of the present invention, the amount of the isopyrazam and metiram may be, for example, 5%, 6%, 7%, 8%, 9%, 10%, 11%, 12%, 13%, 14%, 15%, 16%, 17%, 18%, 19%, 20%, 21%, 22%, 23%, 24%, 25%, 26%, 27%, 28%, 29%, 30%, 31%, 32%, 33%, 34%, 35%, 36%, 37%, 38%, 39%, 40%, 41%, 42%, 43%, 44%, 45%, 46%, 47%, 48%, 49%, 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, 60%, 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, 70%, 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, 90%.

The fungicidal composition of the present invention has a strong activity against various plant pathogenic bacteria, and can exert a strong control effect on the prevention and treatment of plant diseases caused by plant pathogenic bacteria.

The bactericidal composition is used for preventing and treating plant pathogenic fungi on cereals, vegetables, fruits, ornamental plants and grapevines.

The fungicidal compositions are useful for controlling cereal, vegetable, fruit, ornamental and vine ascomycetes (e.g., Anabaena, Podosphaera, Erysiphe, Candida, Mycosphaera, Sphaerotheca, Helminthosporium); basidiomycetes (e.g., camelina, rhizoctonia, puccinia, smut, tilletia); deuteromycetes (e.g., Botrytis, Helminthosporium, Trichosporon, Fusarium, Septoria, Cercospora, Alternaria, Pseudocercospora, Verticillium); oomycetes (e.g., Phytophthora, Peronospora, Pseudoperonospora, Puccinia, Aureobasidium, Pythium, Plasmopara), etc.

The plants of more importance in the present invention are wheat, barley, wheat, potato, rice, cucumber, melon, cabbage, grape, red pepper, green pepper, watermelon, pumpkin, tobacco, citrus, apple, tomato, banana, corn, asparagus, endive, calla, cauliflower, cabbage, konjac, rape, celery, japanese radish, tobacco, onion, green stem vegetable, tomato, eggplant, carrot, green onion, chinese cabbage, potato, lettuce, oilseed rape.

The bactericidal composition is used for controlling wheat leaf rust, apple tip powdery mildew, apple scab, barley powdery mildew, grape gray mold, tomato gray mold, wheat leaf blight, barley net blotch, tomato early blight, grape powdery mildew, barley powdery mildew, wheat tip first rot, wheat take-all, wheat leaf rust, rice sheath blight, wheat glume blight, wheat leaf blight, rice blast, potato early blight, potato late blight, cucumber damping-off, corn smut, grape downy mildew, bean gray mold, wheat brown rust, apple powdery mildew, grape powdery mildew, cucumber powdery mildew and cereal eye spot.

The use of the fungicidal compositions for the protection of plants, plant propagation material and plant organs that grow at a later time.

The application of the bactericidal composition to the site needing control to control pathogenic or saprophytic harmful fungi in soil or culture medium.

The use of the fungicidal composition for the treatment of seeds to protect the seeds from attack by carried phytopathogens.

The use of the germicidal composition for protecting stored objects.

The use of the fungicidal composition for protecting stored goods against fungal or bacterial infestation during storage.

A method for controlling or preventing infestation of cultivated plants by phytopathogenic fungi, which method comprises applying the fungicidal composition according to the invention to the phytopathogenic fungi and/or their environment or to the plants, to plant propagation material and to plant organs, to the soil or to the cultivation medium, to the material or to the space which develops subsequently.

A method of controlling or preventing infestation of cultivated plants by phytopathogenic fungi which comprises applying a fungicidal composition according to the invention to the foliage of the plants.

A method of controlling or preventing infestation of cultivated plants by phytopathogenic fungi which comprises applying a fungicidal composition according to the invention to plant propagation material and to the plant organs which grow thereafter.

A method of controlling or preventing infestation of cultivated plants by phytopathogenic fungi, which method comprises applying a fungicidal composition according to the invention to the soil or to a cultivation medium.

A method of controlling or preventing infestation of a cultivated plant by phytopathogenic fungi, which method comprises applying said fungicidal composition to the phytopathogenic fungi of the plant and/or to its environment, or to the plant, to plant propagation material and to plant organs, soil or cultivation media, materials or spaces which grow at a time before or after the cultivated plant is infested.

A method for controlling or preventing infestation of cultivated plants by phytopathogenic fungi, which comprises applying the fungicidal compositions of the invention in seed treatment, foliar application, stem application, drenching, instillation, pouring, spraying, dusting, scattering or smoking, or the like, to the phytopathogenic fungi and/or to the environment thereof, or to the plants, to plant propagation material and to the organs, the soil or to cultivation media, materials or spaces to be grown subsequently, at an agronomically effective and substantially non-phytotoxic application rate.

A method of controlling or preventing infestation of cultivated plants by phytopathogenic fungi which comprises the simultaneous or sequential application of isopyrazam and metiram.

The fungicidal composition of the present invention has excellent activity against a wide range of phytopathogenic fungi such as basidiomycetes, ascomycetes, oomycetes and deuteromycetes.

Oomycetes, including Phytophthora (Phytophthora), such as Phytophthora infestans (Phytophthora sojae), Phytophthora sojae (Phytophthora sojae. gaspierma), Phytophthora citrus (Phytophthora parasitica), Phytophthora cinnamomi (Phytophthora cinnamomi), and Phytophthora cucurbitae (Phytophthora capsici); diseases of Pythium species (Pythium), such as Pythium turfgrasum fusarium (Pythium aphanidermatum); and diseases of the downy mildew family (Peronosporaceae) such as plasmopara viticola (plasmopara viticola), Peronospora (Peronospora) including Peronospora tabacum (peronosporaabacina) and Peronospora parasitica (Peronospora paradiata), Pseudoperonospora (Pseudoperonospora) including Peronospora cucumerina (Pseudoperonospora cubensis) and bremia (bremia lactucae), Pythium (Pythium) such as Pythium aphanidermatum (Pythium aphanidermatum);

Ascomycetes, including Alternaria (Alternaria) diseases such as tomato early blight (Alternaria) and cabbage black spot (Alternaria) and Mycoplasma brassicae (Alternaria), Mycoplasma (Guignardia) diseases such as grape black rot (Guignardia bidwellii), Venturia (Venturia) diseases such as apple scaber (Venturia nigrella), Septoria (Sepia) diseases such as Septoria nodorum (Septoria nodorum) and leaf blight (Septoria), aleurona (Encelinuria) diseases such as Erysiphe (Erysiphe) including wheat powdery mildew (Erysiphe graminis) and gloiophyllum (Erysiphe), grape powdery mildew (Uncinula cucumerides), powdery mildew (Sphaerothecium) and powdery mildew (Potentilla graminis), Sphaerothecium (Sphaerothecoides), Gracilaria solanacearum (Bochybotrytis), Gracilaria solanacearum (Botrytrium), Gracilaria) diseases such as Botrytrium gramineararia (Botrytis), Gracilaria) and Botrytis (Botrytis) diseases, Grape branch blight (phomopsis viticola) disease, Helminthosporium (Helminthosporium) species disease such as corn northern leaf blight (Helminthosporium), hill scratch pattern (pyrenophores) species, anthrax disease such as black fruit rot (glomeriella) or anthracnose (Colletotrichum) disease (such as sorghum anthracnose (collecticola) and watermelon anthracnose (collectirium), and wheat holothrin (gaeumannomyces graminis);

Basidiomycetes, including rust diseases caused by rusts (Puccinia genus) (e.g., Pucciniarecondita (Pucciniarecondita), Pucciniastriiformis (Pucciniastriiformis), Puccinia (Pucciniahordei), Puccinia (Pucciniagraminis), and Pucciniarachidia (Pucciniaarachidis)), coffee rusts (Hemilaviastatrix) and soybean rusts (Phakopsorapaphyrizi);

deuteromycetes, including Rhizoctonia species (Rhizoctonia species) (e.g., Rhizoctonia solani (Rhizoctonia) and Rhizoctonia erythrorhizogenes (Rhizoctonia oryzae)); fusarium (Fusarium) diseases, for example Fusarium graminearum (Fusarium graminearum), Fusarium candidum (Fusarium moniliforme), Fusarium oxysporum (Fusarium oxysporum, Fusarium moniliforme (Fusarium proliferatum), Fusarium solani (Fusarium solani), Verticillium dahliae (Verticillium dahliae), Rhizoctonia alba (Sclerotiumrolfsii), Phyllomyces maculans (Rynchyosporium), Rhizoctonia nigra (Cercosporium personatum), Fusarium nigrum (Cercosporium nigrum), and Fusarium oxysporum (Cercosporium nigrum), and Fusarium argenteum (Cercosporium purpureum).

The fungicidal compositions of the present invention are particularly effective against the following classes of phytopathogenic fungi: ascomycetes (e.g., anabaena, puccinia, erysiphe, candida, mycosphaerella, leptospora, helminthosporium); basidiomycetes (e.g., camelina, rhizoctonia, puccinia, smut, tilletia); deuteromycetes (e.g., Botrytis, Helminthosporium, Trichosporon, Fusarium, Septoria, Cercospora, Alternaria, Pseudocercospora, Verticillium); oomycetes (e.g., Phytophthora, Peronospora, Pseudoperonospora, Puccinia, Aureobasidium, Pythium, Plasmopara), etc.

The fungicidal compositions of the present invention are suitable for crop plants consisting essentially of: cereal crops such as wheat, barley, oats, rye, triticale, rice, corn, sorghum and millet; vining crops such as fresh grapes and wine grapes; field crops, such as oilseed rape (canola), sunflower; sugar beet, sugar cane, soybean, peanut (groundnut), tobacco, alfalfa, clover, lespedeza, clover, and vetch; pome fruits such as apple, pear, wild apple, loquat, hawthorn and quince; stone fruit, such as peach, cherry, plum, apricot, nectarine; citrus fruits such as lemon, lime, orange, grapefruit, mandarin orange (tangerine), and kumquat; rhizome plants and field crops (and their leaves), such as artichoke, beet and sugar beet, carrot, cassava, ginger, ginseng, horseradish, parsnip, potato, radish, turnip cabbage, sweet potato, turnip and yam; bulb plants such as garlic, leek, onion and shallot; leafy vegetable plants, such as mustard (sesamol), celery, cress, chicory (cogongrass), fennel, lettuce and loose-leaf lettuce, parsley, red chicory (red chicory), rhubarb, spinach and swiss chard; brassica (korean) leafy vegetables such as broccoli, cauliflower (broccoli), brussels sprouts, cabbage, bok choy, cauliflower, cabbage, kale, kohlrabi, mustard and broccoli; bean plants (juicy or juicless) such as lupins, beans) (including fava beans, kidney beans, garden beans, safflower beans, snap beans, broad bean beans), beans (including adzuki beans, asparagus beans, eyebrow beans, cowpea pods, cowpea beans, mung beans, cowpea beans, black mung beans and ultralong cowpea beans), fava beans, chickpeas, guar ears, sword beans, lentils and peas (including kidney beans, snap peas, purple peas, green peas, snow beans, sweet beans, pigeon peas and soybeans); fruit vegetables such as eggplant, cherries, muskmelon eggplant, and hot pepper (including bell pepper, hot pepper, cooking hot pepper, sweet pepper; small tomato, and tomato); cucurbitaceous vegetables such as chayote (fruit), wax gourd, citrullus lanatus, cucumber, squash, edible cucurbits (including cucurbits, cucurbits), cucurbits, okra, gummy balsam pear, momordica charantia, and cucurbits and watermelons; berries, such as blackberry, red berry, dewberry, purple blueberry, cranberry, blackcurrant, wild berry, loganberry, raspberry and strawberry; tree nuts such as almonds, beech nuts, brazil nuts, white walnuts, cashews, chestnuts, hazelnuts (hazelnuts), pecans, macadamia nuts, pecans, and walnuts; tropical fruits and other crops, such as bananas, plantains, mangos, coconut, papaya, avocados, lychees, agave, coffee, cocoa, sugar cane, oil palm, sesame, gums and spices; fiber crops, such as cotton, flax and industrial hemp; turf grass (including warm-season and cool-season turf grass).

Preferred fungicidal compositions of the present invention suitable crop plants generally include plants of the following genera: cereals (wheat, barley, rye, oats, rice, maize, sorghum and related varieties); sugar beet (sugar beet and fodder beet); pomes, stone fruits and berries (apples, pears, plums, peaches, apricots, cherries, strawberries, raspberries and blackberries); leguminous plants (beans, squash beans, peas, soybeans); oil plants (rape, mustard, poppy, olive, sunflower, coconut, castor, cocoa bean, groundnut); cucurbits (squash, cucumber, melon); fiber plants (cotton, flax, industrial hemp, jute); citrus fruit (orange, lemon, grape, citrus); vegetables (spinach, lettuce, asparagus, cabbage, carrot, onion, tomato, potato, red pepper); lauraceae (avocado, cinnamon, camphor) or plants such as tobacco, nuts, coffee, eggplant, sugar cane, tea, pepper, grapes, bananas and natural rubber plants, and ornamental plants.

Crop plants to which the fungicidal compositions of the present invention are particularly applicable include rice, cucumber, melon, cabbage, grape, red pepper, green pepper, watermelon, pumpkin, tobacco, citrus, apple, tomato, banana, corn, asparagus, lettuce, calla, cauliflower, cabbage, konjac, rape, celery, japanese radish, tobacco, onion, green stem, tomato, eggplant, carrot, green onion, chinese cabbage, potato, lettuce, oilseed rape.

The bactericidal composition is particularly suitable for controlling wheat leaf rust, apple tip powdery mildew, apple scab, barley powdery mildew, grape gray mold, tomato gray mold, wheat leaf blight, barley net blotch, tomato early blight, grape powdery mildew, barley powdery mildew, wheat tip first rot, wheat take-all, wheat leaf rust, rice sheath blight, wheat glume blight, wheat leaf blight, rice blast, potato early blight, potato late blight, cucumber damping-off, corn smut, eye spot, grape downy mildew, bean gray mold, wheat brown rust, apple powdery mildew, grape powdery mildew, cucumber powdery mildew and cereal eye spot.

The bactericidal composition can be used as a foliar bactericide in crop protection, and also can be used as a bactericide for seed dressing and a soil bactericide.

The use of the fungicidal compositions of the invention for the protection of plants, plant propagation material and plant organs that grow at a later time.

Use of the fungicidal composition of the invention for the treatment of seeds to protect the seeds from attack by the phytopathogenic fungi carried.

The application of the bactericidal composition to the site needing control to control pathogenic or saprophytic plant pathogens in soil or culture media.

The fungicidal compositions of the present invention are also particularly effective in the prevention or control of seed-borne or soil-borne diseases. Examples of fungal pathogens of the species or soil-borne include Alternaria (Alternaria spp.), Septoria (Ascochyta spp.), Botrytis cinerea (Botrytis cinerea, Cercospora spp.), Claviceps (Claviceps purpurea), Cochlospora graminis (Cochliobolus sativus), Colletotrichum (Colletotrichum spp.), Fusarium graminearum (Fusarium graminearum), Fusarium oxysporum (Fusarium moniliforme), Fusarium moniliforme (Fusarium moniliforme), Fusarium septorium (Fusarium protuberum), Fusarium solanum (Fusarium solanum), Fusarium collodion (Fusarium moniliforme), Fusarium solanum (Fusarium moniliforme), Penicillium (Pyrenophora), Penicillium (Rhizoctonia), Penicillium nivale (Rhizoctonia), Rhizoctonia solani (Rhizoctonia), Fusarium solani (Rhizoctonia), Rhizoctonia (Rhizoctonia), Rhizoctonia solani (Rhizoctonia), Rhizoctonia solani), Rhizoctonia (Rhizoctonia solani), Rhizoctonia solani (Rhizoctonia), Rhizoctonia (Rhizoctonia solani), Rhizoctonia (Rhizoctonia solani), Rhizoctonia (Rhizoctonia solani), Rhizoctonia (Rhizoctonia solani), Rhizoctonia (Rhizoctonia), ustilago virescens (Urocystisoccula), Ustilago spp, Verticillium spp, Phytophthora, Pythium, Peronospora, Pseudoperonospora.

The use of the germicidal composition for protecting stored objects.

The fungicidal compositions of the present invention are also useful for the prevention or control of post-harvest and storage diseases. According to the invention, post-harvest and storage-period diseases can be caused, for example, by the following fungi: colletotrichum species, such as banana Colletotrichum (Colletotrichum musae), Colletotrichum disclinae (Colletotrichum gloeosporioides), capsicum Colletotrichum (Colletotrichum coccodes); fusarium species, such as Fusarium semitectum (Fusarium semitectum), Fusarium moniliforme (Fusarium moniliforme), Fusarium solani (Fusarium solani), Fusarium oxysporum (Fusarium oxysporum); verticillium species, such as Verticillium theobrome (Verticillium theobromae); a species of the genus Neurospora; botrytis species, such as Botrytis cinerea; geotrichum species, such as Geotrichum candidum (Geotrichum candidum); phomopsis species, Phomopisisnatalosis (Phomopisisnalalensis); species of the genus Lasiosphaera, such as, for example, Dichloropsis citrifolia (Diplodia citri); alternaria species, such as, for example, Alternaria citri (Alternaria citri), Alternaria alternata (Alternaria alternata); phytophthora species, such as Phytophthora citri (Phytophthora citrophthora), Phytophthora fragrans (Phytophthora fragaria), Phytophthora infestans (Phytophthora cacorum), Phytophthora nicotianae (Phytophthora parasitica); septoria (Septoria spp.), for example Septoria depressa; mucor spp, such as Mucor piriformis (Mucorpiriformis); streptomyces (Monilinia spp.), such as, for example, Streptomyces fructicola (Monilinia fructicola), Streptomyces drupes (Monilinia laxa); venturia spp, such as Venturia inaequalis, Venturia pyrifera; rhizopus sp, such as Rhizopus stolonifer, Rhizopus oryzae (Rhizopus oryzae); genus Microtheca (Glomeellaspp.), e.g., pericarp (Glomeellacirata); sclerotinia spp, such as Sclerotinia fructicola (Sclerotinia fructicola); the genus longbeak (Ceratocystis spp.), such as the Kiwi long beak (Ceratocystis paradoxa); penicillium spp, such as Penicillium funiculosum (Penicillium funiculosum), Penicillium expansum (Penicillium expandasum), Penicillium digitatum (Penicillium digitatum), Penicillium italicum (Penicillium italicum); pediophora sp, e.g., Pediophora albuginea (Gloeosporium album), Gloeosporium perennans, Pediophora fructicola (Gloeosporium fructigenum), Gloeosporium singulata; the genus chitin of the genus chitin spora (Phlyctaenana spp.), such as Phlyctaenavagabunda; cylindrocarpon spp, such as, for example, Cylindrocarpon mali; stemphylium spp, such as stemphylium citrinum (stemphylium venelicanum); aschersonia (Phacydiopanis spp.), e.g., Phacydiopani malirum; rhizopus (Thielavissis pp.), for example, Rhizopus mirabilis (Thielavissis paradoxy); aspergillus spp, such as Aspergillus niger, Aspergillus carbonarius; genus Lepidium (Nectriaspp.), such as, for example, Lepidium drynarium (Nectria galligena); amycolatopsis (Pezicula spp.).

The bactericidal composition can also be used for preventing and controlling diseases of fruits and vegetables in the storage period, and obtains an unexpected synergistic effect. For example fruit decay caused by the following pathogens:

phytophthora (Phytophthora), such as Phytophthora infestans (Phytophthora infestans), Phytophthora sojae (Phytophthora sojae), Phytophthora citrus foot rot (Phytophthora parasitica);

peronosporaceae (Peronosporae) diseases such as Plasmopara viticola (Plasmoparaviticola), Peronospora (Peronospora);

pythium species (Pythium) such as Pythium aphanidermatum (Pythium aphanidermatum).

The fungicidal compositions according to the invention can also be applied during the growth of plants or plant parts to protect the harvested stock.

The bactericidal composition of the invention is particularly effective in controlling the following plant diseases:

alternaria in fruits and vegetables;

ascochyta in legume crops;

botrytis cinerea (grey mould) in strawberries, tomatoes, sunflowers, legumes, vegetables and grapes;

ananas arachidicola in peanuts;

phyllospora graminis in cereals;

colletotrichum in legume crops;

powdery mildew species in cereals;

erysiphe cichoracearum and Xanthium sibiricum in cucurbits;

Fusarium in cereals and maize;

gaeumannomyces graminis in cereals and lawns; ,

helminthosporium in corn, rice and potatoes;

rust bacteria camelina coffea on coffee;

micronozoies in wheat and rye;

phakopsora species in soybean;

puccinia in cereals, broadleaf crops and perennial plants;

pseudocercospora species in cereals;

short tip rust in roses;

podosphaera species in fruits;

pyrenophora in barley;

rice blast fungus in rice;

rhizoctonia species in cotton, soybean, corn, maize, potato, rice and turf;

zuelan in barley and rye;

sclerotinia in lawn, lettuce, vegetables and oilseed rape;

septoria in cereals, soybeans and vegetables;

ustilago sorghum from maize;

tilletia species in cereals;

leptospermum, globisporus rini and phomopsis viticola in vine plants;

ustilago occulta in rye;

smut in cereals and maize;

venturia in fruit;

candida on fruit;

penicillium on citrus and apple.

The fungicidal compositions of the present invention are particularly effective against post-harvest and shelf-life disease diseases as follows: such as Botrytis cinerea, banana anthracnose, Curvularia lunata, Fusarium semitectum, Geotrichum candidum, Monilinia fructicola, Sclerotinia fructicola, Rhizoctonia fructicola, Mucor pyriformis, Penicillium italicum, Penicillium ionogen.

The bactericidal composition can treat all plants and plant parts. "plant" means all plants and plant populations, such as desirable and undesirable wild plants, cultivars, and plant varieties (whether protected by a plant variety or plant cultivar rights). Cultivated plants and plant varieties may be plants obtained by conventional propagation and cultivation methods, which may be supplemented or supplemented by one or more biotechnological methods, for example using dihaploids, protoplast fusion, random and directed mutations, molecular or genetic markers, or using bioengineering and genetic engineering methods. Plant parts are understood to mean all above-and underground parts and organs of plants, such as shoots, leaves, flowers and roots, for example leaves, needles, stems, branches, flowers, fruit bodies, fruits and seeds, and also roots, bulbs and rhizomes. Also plants and vegetative and generative propagation material, for example cuttings, bulbs, rhizomes, runners and seeds, belong to the plant part.

The term "plant propagation material" is understood to mean all plant parts capable of reproduction, such as seeds, which can be used after reproduction, and also plant material, such as cuttings or tubers (e.g. potatoes). Thus, plant parts as used herein include plant propagation material. Mention may be made, for example, of seeds, roots, fruits, tubers, bulbs, rhizomes and plant parts. Germinated plants and useful plants to be inhibited after germination or after emergence from the soil. The young plants can be protected prior to transplantation by a total or partial treatment by dipping.

A method for controlling or preventing infestation of cultivated plants by phytopathogenic fungi, which comprises applying the fungicidal compositions of the invention in seed treatment, foliar application, stem application, drenching, instillation, pouring, spraying, dusting, scattering or smoking, to the phytopathogenic fungi and/or to the environment thereof, or to the plants, parts of plants, plant propagation material and to the organs of plants, the soil or the cultivation media, the materials or the spaces which grow at a rate which is agronomically effective and substantially non-phytotoxic.

A method of controlling or preventing infestation of cultivated plants by phytopathogenic fungi which comprises the simultaneous application, or the sequential application, of isopyrazam and metiram.

A method for controlling or preventing infestation of cultivated plants by phytopathogenic fungi by application of said fungicidal composition to plant propagation material and to plant organs which grow subsequently.

Preferred plant propagation material of the present invention is a seed. The fungicidal compositions of the present invention are also particularly suitable for the treatment of seeds. Most of the crop damage caused by harmful fungi is caused by the attack of the seeds during storage or after sowing and during or after germination of the plants. Plants are particularly sensitive to roots and shoots during the growing period and can cause death of the plant even if there is little damage.

Another aspect of the present invention provides a method for protecting seeds and germinating plants, which method makes it unnecessary or at least significant additional application of crop protection agents after sowing or after the emergence of the plants. On the other hand, the amount of active compound used is optimized with the fungicidal composition according to the invention in order to provide maximum protection of the seeds and germinating plants from attack by phytopathogenic fungi, without the plants themselves being harmed by the active compound used.

The present invention therefore also relates in particular to a method for protecting seeds and germinating plants from attack by phytopathogenic fungi by treating the seeds with a fungicidal composition according to the present invention. The invention also relates to the use of the fungicidal compositions according to the invention for the treatment of seeds to protect the seeds and the germinating plants from phytopathogenic fungi.

The control of phytopathogenic fungi which damage the post-emergent plants is effected primarily by treating the soil and the aerial parts of the plants with crop protection agents. In view of the possible effects of crop protection agents on the environment and on the health of humans and animals, it is therefore necessary to minimize the application rates of the active compounds.

The fungicidal compositions according to the invention are suitable for protecting the seed of any plant variety applied in agriculture, in greenhouses, in forestry or in horticulture-or grape cultivars. In particular, it takes the form of seeds of cereals (such as wheat, barley, rye, triticale, millet, oats), soya, sorghum, peas, lentils, maize, cotton, soya, rice, potatoes, sunflowers, beans, coffee, sugar beet, peanuts, rape, olives, cocoa, sugar cane, tobacco, vegetables (such as tomatoes, cucumbers, onions and lettuce), turf grasses and decorative plants. The treatment of cereal and vegetable seeds is of vital importance.

The active ingredients of the isopyrazam and metiram in the bactericidal composition of the invention are applied to seeds alone or in a suitable formulation. It is preferably treated in a sufficiently stable state that the treatment does not cause any damage to the seed. In general, the treatment of the seeds can be carried out at any point in time between picking and sowing. The seeds commonly used are isolated from the plant and from the cob, husk, stem, cuticle, hair or pulp. Thus, for example, seeds that have been picked, cleaned and dried to a moisture content of less than 15% may be used. Alternatively, seeds may be used which are dried, for example by treatment with water, and then dried again.

Examples of the method of seed treatment include a method of diluting a liquid or solid chemical, a method of immersing seeds in a liquid solution without dilution to allow the chemical to permeate the seeds, a method of mixing a solid chemical or liquid chemical with seeds and coating the seeds to allow the chemical to adhere to the surfaces of the seeds, and a method of spraying the chemical to the vicinity of the seeds while planting.

A plant part and plant organ that subsequently grows is any part of a plant produced from plant propagation material, such as seeds. Plant parts, plant organs and plants may also benefit from the pathogenic damage protection obtained by applying the fungicidal composition to plant propagation material. Certain plant parts and plant organs that grow after certain locations may also be considered plant propagation material, which itself may be applied (or treated) with the fungicidal composition; thus plants, other plant parts and other plant organs produced from the treated plant parts and treated plant organs may also benefit from the application of the germicidal composition.

The application of the bactericidal composition to the site needing control to control pathogenic or saprophytic fungi and bacteria in soil or culture medium.

A method for preventing or controlling the infection of cultivated plants by phytopathogenic fungi by applying said fungicidal composition to the soil or to the cultivation medium.

Under general conditions, soil pathogenic bacteria can generate a large amount of bacteria, as long as conditions are favorable for growth and development of the pathogenic bacteria and hosts are susceptible to diseases, the pathogenic bacteria can propagate in a large amount and infect the hosts, under the host infected with the diseases, the pathogenic bacteria can enter a continuous pathogenic period, propagate and diffuse in a large amount along with continuous cropping of crops, but then nutrients are consumed, or when soil conditions such as temperature, humidity and the like are unfavorable for the pathogenic bacteria, the pathogenic bacteria can enter a dormant period. When the host with disease does not exist, soil-borne disease bacteria can survive in soil, and the soil-borne disease bacteria can survive on the root surface or the fallen leaves of the non-host except the soil-borne disease bacteria with wide host range and have the saprophytic competitive ability. However, different germs are different, and like fusarium can almost survive in soil indefinitely.

The culture medium of the present invention refers to a support capable of rooting and growing crops, such as: examples of the raw material include sand, pumice, vermiculite, diatomaceous earth, agar, gel, polymer, asbestos, wood chips, and bark.

Examples of methods for applying a chemical to soil include a method in which a liquid chemical is diluted in water or applied without dilution directly to the roots of a plant or a seedling bed for raising seedlings, a method in which granules are sown to the roots of a plant or a seedling bed for raising seedlings by spraying a powder, a water dispersible granule or the like to soil and mixing with the whole soil before sowing, and a method in which a powder, a water dispersible granule or the like is diluted and sprayed to planting holes or planting furrows before sowing or before planting a plant, and sowing is performed.

The treatment method according to the invention can also be used to protect stored products from fungal and microbial attack. According to the invention, the term "stock" is understood to mean natural substances and processed forms thereof of plant or animal origin which have been derived from the natural life cycle and are intended to be preserved for a long period of time. Storage products of plant origin, for example plants or parts thereof, such as stems, leaves, tubers, seeds, fruits or grains, can be protected in the freshly harvested state or in processed form, such as (pre) drying, wetting, comminuting, grinding, pressing or baking. Or wood, in the form of coarse wood such as construction timber, utility poles and fences; or in finished form, such as furniture or articles made of wood. The animal-derived stock is hide, leather, hair, etc. The compositions according to the invention can prevent fungal or bacterial attack such as corrosion, discoloration or mildew on storage. "stock" is preferably understood as meaning natural substances of plant origin and processed forms thereof, more preferably fruits and processed forms thereof, such as pomes, stone fruits, stone-free small fruits and citrus fruits and processed forms thereof.

The present invention provides a method for controlling or preventing infestation of cultivated plants by phytopathogenic fungi, which comprises applying the fungicidal compositions of the invention in an agronomically effective and substantially non-phytotoxic application rate to the phytopathogenic fungi and/or to the environment thereof, or to the plants, plant propagation material and to the organs, soil or cultivation media, materials or spaces of the plants to be grown subsequently, in a method of seed treatment, foliar application, stem application, drenching, drip, pouring, spraying, misting, dusting, scattering or fuming.

The germicidal compositions of the present invention may be applied by various treatment methods, such as:

-spraying a liquid comprising the fungicidal composition onto the above-ground parts of the plant;

-dusting, incorporating granules or powders in the soil, spraying around said plants and, in the case of tree injection or painting;

-coating or film coating the seeds of the plants.

When the preservative is used for preservation and fresh-keeping of picked fruits and vegetables, the preservative is usually diluted by water by 200 times and 2000 times, and the fruits are leached out after being soaked.

The present invention provides a method of controlling or preventing infestation of cultivated plants by phytopathogenic fungi, either by treatment, prevention or root removal.

A method for controlling or preventing infestation of cultivated plants by phytopathogenic fungi by application of the fungicidal composition to the phytopathogenic fungi and/or their environment, or to the plants, to propagation material of plants and to plant organs, to the soil or to cultivation media, to materials or to spaces which grow at a later time, before or after infestation of the plants.

Treatment according to the invention may produce superadditive ("synergistic") effects. For example, according to the application rate of the germicidal composition used in the present invention and/or widening the activity range and/or increasing the activity thereof, it is possible to obtain the following effects: better plant growth, increased tolerance to high or low temperatures, increased tolerance to drought or to water or soil salt content, improved flowering performance, easier harvesting, accelerated ripening, higher harvest yields, larger fruits, higher plant height, greener leaf color, earlier flowering, higher quality or nutritional value of the harvested product, higher sugar concentration in the fruit, better storage stability and/or processability of the harvested product, which exceeds the actually predicted effects.

The treatment method of the invention may also be used to treat propagation material such as tubers or rhizomes, and may be used to treat seeds, seedlings or transplanted (packing out) seedlings and plants or transplanted plants. This processing method can also be used to process roots. The treatment method of the present invention can also be used for treating the above-ground parts of plants such as the stems, stems or stalks, leaves, flowers and fruits of the plants concerned.

The amount of the germicidal composition of the present invention used depends on various factors, such as the compound used; objects of treatment, such as plants, soil or seeds; the type of treatment, e.g. spraying, dusting or dressing; for treatment purposes, e.g., prophylaxis or therapy; the type of fungus to be controlled or the application time.

Typically for leaf treatment: 5 to 2000g/ha, preferably 10 to 1000g/ha, more preferably 10 to 300 g/ha; for irrigation or drip application, the dosage may even be reduced, particularly when applying inert substrates such as asbestos or perlite;

-for seed treatment: 2-2500g per 100kg of seeds, preferably 3-1000g per 100kg of seeds, more preferably 5-500g per 100kg of seeds, even more preferably 5-250g per 100kg of seeds.

-applying a treatment to the soil or water surface: 0.1 to 10000g/ha, preferably 1 to 5000 g/ha.

For the preservation of picked fruits and vegetables, 200 times and 2000 times of liquid can be diluted, and the fruits can be drained after being soaked.

The isopyrazam and metiram combination/co-administration of the invention. Comprising separate, sequential or simultaneous administration of isopyrazam and metiram. Preferably, the isopyrazam and metiram combination is in the form of a composition comprising isopyrazam and metiram.

The compositions of the present invention may be presented primarily in the form of a preparation, i.e., the components of the composition are already mixed, or the components of the composition may be provided in a single dose, mixed in a tank or container prior to use, and then diluted to the desired concentration. The preparation form provided by the invention is preferably the main form.

The fungicidal compositions of the present invention may be used in any conventional form, including aerosols, capsule suspensions, cold fogging concentrates, hot fogging concentrates, capsule granules, fine granules, ready-to-use solutions, sprayable powders, emulsifiable concentrates, oil-in-water emulsions, water-in-oil emulsions, macro granules, micro granules, oil dispersible powders, oil miscible flowable concentrates, oil miscible liquids, foams, pastes, suspension concentrates, soluble concentrates, suspensions, seed coatings, wettable powders, water dispersible granules, soluble powders, microcapsule suspensions, coated granules, extruded granules, emulsifiable concentrates, microemulsions, aqueous emulsions, effervescent tablets, ultra low volume liquids, suspoemulsions, ultra low volume cold fogging formulations, ultra low volume hot fogging formulations, twin packs (twin packs), dry powders for seed treatments, emulsion for seed treatments, dry powders for seed treatments, Seed treatment suspending agent, seed treatment liquid agent, seed treatment dispersible powder, seed treatment microcapsule suspending agent, seed treatment gel, suspoemulsion and emulsion granules.

The sterilization composition comprises isopyrazam, metiram, a filling agent and/or a surfactant.

The content of the isopyrazam and the metiram in the bactericidal composition accounts for 5% -90% of the bactericidal composition.

The content of the isopyrazam and the metiram in the bactericidal composition accounts for 10% -80% of the bactericidal composition.

The content of the isopyrazam and the metiram in the bactericidal composition accounts for 20-70% of the bactericidal composition.

According to the present invention, the term "filler" refers to a natural or synthetic organic or inorganic compound that can be combined or associated with an active compound to make it easier to apply to a subject (e.g. plants, crops or grasses). Thus, the filler is preferably inert, at least should be agriculturally acceptable. The filler may be solid or liquid.

The inactive medium that can be used in the present invention may be either solid or liquid, and examples of the solid medium material include: examples of the inorganic filler include plant powders (for example, particles of soybean powder, starch, cereal powder, wood powder, bark powder, saw dust, walnut shell powder, bran, cellulose powder, coconut shell, corn cob, and tobacco stalk, and residues after extraction of plant essence), paper, saw dust, synthetic polymers such as ground synthetic resins, clays (for example, kaolin, bentonite, and acid china clay), and talc powders. Silica (for example, diatomaceous earth, silica sand, mica, hydrous silicic acid, calcium silicate), activated carbon, natural minerals (for example, pumice, attapulgite, zeolite, etc.), calcined diatomaceous earth, sand, plastic media (for example, polyethylene, polypropylene, polyvinylidene chloride, etc.), inorganic mineral powders such as potassium chloride, calcium carbonate, calcium phosphate, etc., chemical fertilizers such as ammonium sulfate, ammonium phosphate, urea, ammonium chloride, etc., and soil fertilizers, and these may be used alone or in combination of 2 or more.

Examples of the liquid vehicle material that can be used include water, alcohols (e.g., methanol, ethanol, isopropanol, butanol, and ethylene glycol), ketones (e.g., acetone, methyl ethyl ketone, diisobutyl ketone, and cyclohexanone), ethers (e.g., diethyl ether, dioxane, methyl cellulose, and tetrahydrofuran), aliphatic hydrocarbons (e.g., kerosene, and mineral oil), aromatic hydrocarbons (e.g., benzene, toluene, xylene, mineral spirits, alkyl naphthalenes, chlorinated aromatic hydrocarbons, chlorinated aliphatic hydrocarbons, and chlorobenzene), halogenated hydrocarbons, amides, sulfones, dimethyl sulfoxide, mineral and vegetable oils, and animal oils.

Examples of the surfactant that can be used for emulsifying, dispersing, and/or wetting the active ingredient compound include polyacrylates such as fatty alcohol polyoxyethylene ether, polyoxyethylene alkylaryl ether, polyoxyethylene higher fatty acid ester, phosphoric acid ester of polyoxyethylene alcohol or phenol, fatty acid ester of polyhydric alcohol, alkaryl sulfonic acid, naphthalene sulfonic acid polymer, lignosulfonate, branched polymer of high molecular comb, butyl naphthalene sulfonate, alkylaryl sulfonate, sodium alkylsulfosuccinate, fats and oils, condensates of fatty alcohol and ethylene oxide, and alkyltaurate, and protein hydrolysates. Suitable oligosaccharides or polymers are based, for example, on ethylene monomers, acrylic acid, polyoxyethylene and/or polyoxypropylene alone or in combination with, for example, (poly) alcohols or (poly) amines.

For dispersion stabilization, attachment and/or binding of the active ingredient compounds, auxiliaries such as xanthan gum, magnesium aluminum silicate, gelatin, starch, cellulose methyl ether, polyvinyl alcohol, polyvinyl acetate and natural phospholipids (such as cephalin and lecithin) as well as synthetic phospholipids, bentonite, sodium lignosulfonate and the like can be used.

Wherein the antifreezing agent can be selected from ethylene glycol, propylene glycol, glycerol, and sorbitol. As the deflocculant for the suspendable product, an auxiliary such as a naphthalenesulfonic acid polymer, a polymeric phosphate, or the like can be used.

As the defoaming agent, a silicone defoaming agent can be used.

Colorants which may be used, for example, inorganic pigments such as iron oxide, titanium oxide and prussian blue; and organic pigments/dyes: alizarin dyes, azo dyes, and metal phthalocyanine dyes; and trace elements such as iron, manganese, boron, copper, cobalt, molybdenum and zinc salts.

Optionally, further additional components, such as protective colloids, binders, thickeners, thixotropic agents, penetrating agents, stabilizers, masking agents, can also be included.

The formulations of the invention can be prepared by mixing the active compounds with the customary additives in a known manner. Such as conventional extenders as well as solvents or diluents, emulsifiers, dispersants, and/or binders or fixatives, wetting agents, water repellents, if desired siccatives and colorants, stabilizers, pigments, defoamers, preservatives, thickeners, water and other processing aids.

These compositions include not only compositions that are immediately applicable to the subject to be treated by means of a suitable device, such as a spraying or dusting device, but also concentrated commercial compositions that require dilution prior to application to the subject.

The isopyrazam-and metiram-containing formulations of the invention can also be administered in combination with other active ingredients, for example for the purpose of broadening the spectrum of activity or preventing the development of resistance. Such as fungicides, bactericides, attractants, insecticides, acaricides, nematicides, growth regulators, herbicides, safeners, fertilizers or semiochemicals and the like.

The active ingredients in the fungicidal compositions of the invention can also be used as such or in their formulations in admixture with known fungicides, bactericides, acaricides, nematicides or insecticides, in order, for example, to broaden their spectrum of action or to prevent the development of resistance.

Such as azoxystrobin, mepanipyrim, fluoxastrobin, kresoxim-methyl, metominostrobin, orysastrobin, pyribenzoxastrobin, pyraclostrobin, trifloxystrobin, epoxiconazole, bromuconazole, cyproconazole, difenoconazole, diniconazole-M, epoxiconazole, fenbuconazole, fluquinconazole, flusilazole, flutriafol, hexaconazole, imazalil, ipconazole, metconazole, myclobutanil, imidazole, pefurazoate, penconazole, prochloraz, propiconazole, prothioconazole, simeconazole, tebuconazole, tetraconazole, triadimefon, triadimenol, triflumizole, imazole, benzytriazole, hexaconazole, cyproconazole, furconazole, and carzolidone, fenpiclonitrile, fludioxonil, cyprodinil, pyrimethanil, dodecamorph, fenpropimorph, tridemorph, fenpropimorph, fenpropiconazole, spirotetramine, fenflurazole, cyhalothrin, mepiquat, tridylate, cyhalothrin, tridyn, cyhalothrin, chlorothalonil, famoxadone, fenamidone, benomyl, carbendazim, thiabendazole, thiophanate-methyl, iprodione, procymidone, vinclozolin, bitertanol, fluroxypyr, metalaxyl-M, furamide, oxadixyl, Iprobenfos (IBP), isoprothiolane, carboxin, methylfuroamide, flutolanil, furazolidone, mepronil, carboxin, thifluzamide, bupirimate, ethirimol, diethofencarb, quinoxyfen, dimethomorph, cyam, tetrachlorophthalide, pyroquilon, tricyclazole, fenhexamid, polyoxin, pencycuron, cyazofamid, zoxamide, blasticidin, kasugamycin, cyazofamid, propamocarb, thiobencarb, fluazinam, fentin chloride, oxolinic acid, octocrythrone, captan, capton, copper caprylate, copper hydroxide, benfluanid, dithianon, folpet, guazate, iminoctadine, mancozeb, maneb, metiram, thiram, zineb, iprovalicarb, fluopyram, pyraclostrobin, sulfcarb, silthiopham, bacillus subtilis, dichloropropene, iprovalicarb, polyoxin, boscalid, hexaconazole, penthiopyrad, paclobutrazol, abamectin, beta-cypermethrin, amitraz, benomyl, bifenazate, bifenothrin, brommethrin, bromophos, bromopropylate, pyridaben, tiazamide, imazamox, acaricidal ether, chlorfenapyr, fenapyr, mitic, miticide, chlorpyrifos, cypermethrin, diafenthiuron, dicofol, dinitrocresol, fenpropathrin, fenvalerate, flufenvalerate, flufenpyrazox, flufenpyroximate, fenvalerate fluoride, flufenoxuron, flumethrin, thiacloprid, ivermectin; lufenuron, methomyl, methyl bromide, metolcarb, tolfenphos, milbemectin, oxamyl, parathion, profenofos, pyridaben, pyridaphethione, rotenone, spirodiclofen, spiromesifen, flubendiamide, tebufenpyrad, triazophos, abamectin, emamectin benzoate, ivermectin, copper hydroxide, kasugamycin, cue lure, eugenol, abamectin, acetamiprid, chlorfenapyr, chlorpyrifos-methyl, tebufenozide, cyhalothrin; clothianidin, imidacloprid; indoxacarb, isoprothiolane, ivermectin, lufenuron, fosthiazate, methoxyfenozide, milbemectin, nitenpyram, nithiazide, oxamyl, parathion; methyl parathion, chlorfluazuron, pentachlorophenol, phenothrin, phenthoate and phorate; profenofos, fluthrin, pymetrozine, pyridaben, pyridaphenthion, spinosad, spiromesifen, sulfluramid, thiamethoxam, thiocyclam, thiodicarb, dimehypo, tolfenpyrad, triflumuron, abamectin, benomyl, pyridaben, carbofuran, chlorpyrifos and metam.

The active compounds isopyrazam and metiram can be applied simultaneously, or separately, or sequentially, the order of separate application usually having no effect on the results of the control.

The germicidal composition of the present invention may be formulated primarily, i.e., the materials in the composition are already mixed, or the components of the composition may be provided in a single dose, mixed in a tank or tank prior to use, and then diluted to the desired concentration. The preparation form provided by the invention is preferably the main form.

The fungicidal compositions of the invention have improved activity against harmful fungi at a reduced total amount of active compound applied (synergistic). The bactericidal composition of the present invention also has an excellent bactericidal effect against bacteria that exhibit resistance to existing bactericides.

According to the bactericidal composition, the isopyrazam and the metiram are combined, so that the obtained composition has a gain effect on the prevention and treatment effect, the bactericidal spectrum is expanded, the multi-purpose effect of one medicine is achieved, and the drug resistance of germs is effectively slowed down or avoided. Surprisingly, the fungicidal activity of the fungicidal compositions according to the invention is significantly higher than the sum of the activities of the individual active compounds, there being an unpredictable, truly occurring synergistic effect, not merely a supplementation of the activities.

The bactericidal composition has the surprising effect of plant physiological effect besides the fungicidal synergistic effect. The plant physiology comprises:

abiotic stress resistance, including temperature resistance, drought tolerance and recovery after drought stress, water use efficiency (associated with reduced water consumption), flood resistance, ozone stress and UV resistance, resistance to chemical substances such as heavy metals, salts, pesticides (safeners) and the like.

Biotic stress resistance, including increased fungal resistance and increased resistance to nematodes, viruses and bacteria.

Increased plant vigor, including plant health/plant mass and seed vigor, reduced toppling, improved appearance, increased recovery, improved greening effect, and improved photosynthetic efficiency.

The bactericidal composition expands the action range of the isopyrazam and the metiram in at least two aspects. Firstly, the application rates of isopyrazam and metiram are reduced, and the effect is still kept good. Secondly, the combination of isopyrazam and metiram still achieves a high degree of phytopathogen control even if the individual compounds become completely ineffective at low application rates. On one hand, the spectrum of the plant pathogens which can be prevented and controlled is expanded, and on the other hand, the use safety is improved.

Detailed Description

The invention will be further illustrated with reference to the following examples:

formulation examples

Example 112% isopyrazam + 18% metiram wettable powder

Mixing the active ingredients, various auxiliary agents, fillers and the like according to the proportion of the formula, and crushing the mixture by a superfine crusher to obtain the 12 percent isopyrazam and 18 percent metiram wettable powder.

Example 22% isopyrazam + 3% Thiazolidone emulsion

The components are prepared according to the proportion and are stirred uniformly to obtain a uniform phase.

Example 315% isopyrazam + 20% metiram water dispersible granule

Uniformly mixing the isopyrazam, the metiram active ingredient, the dispersing agent, the wetting agent, the disintegrating agent and the filler according to the proportion of the formula, and crushing the mixture into wettable powder by air flow; adding a certain amount of water, mixing and extruding to obtain the finished product. After drying and screening, 15% isopyrazam and 20% metiram water dispersible granules are obtained.

Example 46% isopyrazam + 18% Thiazolidine suspoemulsion

Oil phase:

metiram 18%

SOLVESSO^TM200 10％

Ethoxylated castor oil 5%

Water phase:

isopyrazam 6%

Sodium salt of sulfonated naphthalene sulfonic acid-formaldehyde condensation product 1%

The water is complemented to 100 percent

Dissolving isopyrazam in SOLVESSO^TM200, adding ethoxylated castor oil to obtain an oil phase; grinding and/or high-speed shearing sodium salt and water of a condensation product of metiram and sulfonated naphthalene sulfonic acid-formaldehyde according to a formula to obtain a metiram aqueous suspension agent; adding the oil phase to the aqueous phase under stirring to obtain a suspoemulsion.

Example 52% isopyrazam + 8% metiram coated granules

The finely ground active ingredient is uniformly coated onto the carrier moistened with polyethylene glycol in a mixer. In this way coated granules are obtained.

Example 620% isopyrazam + 10% metiram extruded granules

The active ingredient is mixed with the adjuvant and milled, and the composition is moistened with water. The composition is extruded and then dried in an air stream.

Example 730% isopyrazam + 10% metiram suspension seed coating

The seed coating agent is obtained by mixing the components in proportion and grinding and/or high-speed shearing.

Example 825% isopyrazam + 5% metiram dispersible oil suspension

The dispersible oil suspending agent is obtained by mixing the components in proportion and grinding and/or shearing at a high speed.

Example 95% isopyrazam + 5% metiram microcapsule suspension-suspension

Capsule core:

5 percent of isopyrazam

Plurafac LF1312(from BASF) 3％

Emulsogen 3510(from Clariant) 1％

Capsule wall:

M20S(BASF Elastogran) 3.5％

DETA(BASF SE) 1.5％

water phase:

will be provided with

Adding oil phase formed by M20S, isopyrazam, Plurafac LF1312 and Emulsogen 3510 into oil phase containing DETA, beta-cyclodextrin, and beta-cyclodextrin,

4913 in an aqueous solution, an emulsion is formed. Then the catalyst is added and reacted for 2 hours at 50 ℃ while heating and maintaining the temperature. Cooling to obtain the microcapsule of the isopyrazam.

Metiram, Synperonic PE/64(from Croda), a defoaming agent, urea and water are mixed uniformly according to a certain proportion and are sanded to prepare an aqueous suspension.

Adding the microcapsule containing the isopyrazam into the water suspending agent containing metiram to obtain the microcapsule suspension-suspending agent.

Example 1011.2% isopyrazam + 17.8% metiram suspension

11.2 percent of isopyrazam

17.8 percent of metiram

The active components, the dispersant, the wetting agent, the water and the like are uniformly mixed according to the proportion of the formula, and the mixture is ground and/or sheared at high speed, and the particle size is controlled to be below 5 mu m, so that the 11.2 percent isopyrazam and 17.8 percent metiram suspending agent are obtained.

Example 110.5% isopyrazam + 1.5% metiram Electrostatic oil

Mixing the above components, and stirring to obtain transparent homogeneous phase.

Example 1230% isopyrazam + 50% metiram Water dispersible granules

Uniformly mixing the isopyrazam, the metiram active ingredient, the dispersing agent, the wetting agent, the disintegrating agent and the filler according to the proportion of the formula, and crushing the mixture into wettable powder by air flow; adding a certain amount of water, mixing and extruding to obtain the finished product. And drying and screening to obtain 30% isopyrazam and 50% metiram water dispersible granules.

Example 1313% isopyrazam + 17% Thiazolam in Water emulsion

Oil phase:

water phase:

dissolving isopyrazam and metiram in methyl oleate, and adding polystyrene to obtain an oil phase; uniformly mixing the components in the formula to obtain a water phase; and adding the oil phase into the water phase under stirring to obtain the aqueous emulsion.

Example 145% isopyrazam + 10% metiram microemulsion

Example 1540% isopyrazam + 60% metiram

Isopyrazam 40%

Metiram 60%

Mixing isopyrazam and metiram uniformly according to a proportion.

Example 1660% isopyrazam + 40% metiram

Isopyrazam 60%

Metiram 40%

Mixing isopyrazam and metiram uniformly according to a proportion.

The proportion in the above examples is weight percent.

Biological test example

Firstly, toxicity test:

calculating the virulence index of each medicament and the cotoxicity coefficient (CTC value) of the mixture by a Sun Yunpei method, wherein when the CTC is less than or equal to 80, the composition shows antagonism, when the CTC is less than 80 and less than 120, the composition shows additivity, and when the CTC is more than or equal to 120, the composition shows synergism.

The observed virulence index (ATI) ═ 100 (standard agent EC 50/test agent EC 50).)

Theoretical virulence index (TTI) ═ A agent virulence index: (percentage of A in the mixture + B agent virulence index: (percentage of B in the mixture)

Co-toxicity coefficient (CTC) ═ mix measured toxicity index (ATI)/mix Theoretical Toxicity Index (TTI) × 100

Plant pathogens and host plants for use in greenhouse virulence tests

Host plant	Disease name	Pathogenic organisms
			Grape	Gray mold of grape	Botrytis cinerea (Botrytis cinerea)
Wheat (Triticum aestivum L.)	Wheat leaf blight	Septoria tritici (Miq.) Kuntze
			Rice (Oryza sativa L.) with improved resistance to stress	Blast of rice	Magnaporthe grisea
Potato	Late blight of potato	Phytophthora infestans
			Barley	Net blotch of barley	Pyrenophora teres (Fr.) karst
Apple (Malus pumila)	Scab of apple	Venturia apple (Fr.) Pilat
			Wheat (Triticum aestivum L.)	Bacterial glume of wheat	Mycosphaerella graminicola (Lem.) Kuntze
Corn (corn)	Smut of corn	Pinctada yushuensis
			Cucumber (Cucumis sativus)	Damping-off of cucumber	Pythium ultimum
Grape	Downy mildew of grape	Plasmopara viticola
			Barley	Powdery mildew of barley	Powdery mildew of barley
Wheat (Triticum aestivum L.)	Brown rust of wheat	Puccinia recondita (Leptochles tritici)
			Tomato	Early blight of tomato	Alternaria solani
Grape	Powdery mildew of grape	Uncaria rhynchophylla (pers.) hand. -Mazz
			Cucumber (Cucumis sativus)	Powdery mildew of cucumber	Podosphaera leucotricha (wall.) Kuntze

Test one: toxicity assay for botrytis cinerea

Adopting a method for inhibiting the growth rate of hypha:

dissolving isopyrazam and metiram respectively with acetone, diluting with 0.1% Tween-80 aqueous solution to prepare liquid medicines with series concentrations, respectively sucking 6mL into sterilized triangular flasks in a super clean bench, adding 54mL of potato glucose agar (PDA) culture medium at about 50 ℃, shaking uniformly, pouring into 4 dishes with diameters of 9cm, and making into 4 toxic culture media with corresponding concentrations; the compound liquid medicine with different proportions of isopyrazam and metiram series concentration is prepared into a toxic culture medium by the same method. The botrytis cinerea cultured for 2 days is beaten into bacterial blocks at the edges of bacterial colonies by a puncher with the diameter of 5mm, the bacterial blocks are transferred to the center of a prepared toxic PDA culture medium by an inoculation needle, and then the bacterial blocks are cultured in an incubator at 25 ℃, and each treatment is repeated for 4 times. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was determined by correction. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:

Then calculating the concentration EC in the inhibition by using a least square method₅₀Then, the cotoxicity coefficient (CTC) was calculated by the Sun Yunpei method.

Table 1: toxicity test results on Botrytis cinerea

As can be seen from Table 1, when the combination of isopyrazam and metiram is in the range of the mixture ratio of 10:1-1:50, the number of co-toxicity coefficients to Botrytis cinerea is more than 120, and the synergistic effect is shown.

And (2) test II: toxicity assay for septoria tritici

Adopting a method for inhibiting the growth rate of hypha:

dissolving isopyrazam and metiram respectively with acetone, diluting with 0.1% Tween-80 aqueous solution to prepare liquid medicines with series concentrations, respectively sucking 6mL into sterilized triangular flasks in a super clean bench, adding 54mL of potato glucose agar (PDA) culture medium at about 50 ℃, shaking uniformly, pouring into 4 dishes with diameters of 9cm, and making into 4 toxic culture media with corresponding concentrations; the compound liquid medicine with different proportions of isopyrazam and metiram series concentration is prepared into a toxic culture medium by the same method. The wheat septoria tritici cultured for 2 days is beaten into fungus blocks at the edges of the colonies by a puncher with the diameter of 5mm, the fungus blocks are transferred to the center of a prepared toxic PDA culture medium by an inoculation needle, and then the fungus blocks are placed in an incubator at 25 ℃ for culture, and the treatment is repeated for 4 times. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was determined by correction. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:

Table 2: toxicity test result on septoria tritici

As can be seen from Table 2, the combination of isopyrazam and metiram shows a synergistic effect when the number of co-toxicity coefficients to septoria tritici is greater than 120 in the range of the ratio of 10:1 to 1: 50.

Experiment three: indoor toxicity determination of isopyrazam and metiram on rice blast

Adopting a method for inhibiting the growth rate of hypha:

dissolving isopyrazam and metiram respectively with acetone, diluting with 0.1% Tween-80 aqueous solution to prepare liquid medicines with series concentrations, respectively sucking 6mL into sterilized triangular flasks in a super clean bench, adding 54mL of potato glucose agar (PDA) culture medium at about 50 ℃, shaking uniformly, pouring into 4 dishes with diameters of 9cm, and making into 4 toxic culture media with corresponding concentrations; the compound liquid medicine with different proportions of isopyrazam and metiram series concentration is prepared into a toxic culture medium by the same method. The rice blast cultured for 2 days was beaten into a block at the edge of the colony with a punch having a diameter of 5mm, the block was transferred to the center of a previously prepared PDA medium containing a toxin with an inoculating needle, and then cultured in an incubator at 25 ℃ for 4 times per treatment. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was determined by correction. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:

Table 3: test results on the virulence of rice blast

As can be seen from Table 3, when the combination of isopyrazam and metiram is in the range of the mixture ratio of 10:1 to 1:50, the co-toxicity coefficient to rice blast fungus is more than 120, and the synergistic effect is shown.

Experiment four: indoor toxicity determination of isophthora infestans by isopyrazam and metiram

Adopting a method for inhibiting the growth rate of hypha:

dissolving isopyrazam and metiram respectively with acetone, diluting with 0.1% Tween-80 aqueous solution to prepare liquid medicines with series concentrations, respectively sucking 6mL into sterilized triangular flasks in a super clean bench, adding 54mL of potato glucose agar (PDA) culture medium at about 50 ℃, shaking uniformly, pouring into 4 dishes with diameters of 9cm, and making into 4 toxic culture media with corresponding concentrations; the compound liquid medicine with different proportions of isopyrazam and metiram series concentration is prepared into a toxic culture medium by the same method. Phytophthora infestans cultured for 2 days was punched into a block at the edge of the colony using a punch having a diameter of 5mm, the block was transferred to the center of a previously prepared PDA medium containing a toxin using an inoculating needle, and then cultured in an incubator at 25 ℃ with 4 repetitions of each treatment. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was corrected. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:

Table 4: virulence test results against Phytophthora infestans

As can be seen from Table 4, when the combination of isopyrazam and metiram is in the range of the mixture ratio of 10:1-1:50, the co-toxicity coefficient to Phytophthora infestans is greater than 120, and the synergistic effect is shown.

Experiment five: indoor toxicity determination of isopyrazam and metiram on pyrenophora teres

Adopting a method for inhibiting the growth rate of hypha:

dissolving isopyrazam and metiram respectively with acetone, diluting with 0.1% Tween-80 aqueous solution to prepare liquid medicines with series concentrations, respectively sucking 6mL into sterilized triangular flasks in a super clean bench, adding 54mL of potato glucose agar (PDA) culture medium at about 50 ℃, shaking uniformly, pouring into 4 dishes with diameters of 9cm, and making into 4 toxic culture media with corresponding concentrations; the compound liquid medicine with different proportions of isopyrazam and metiram series concentration is prepared into a toxic culture medium by the same method. 2 days of cultured Pyrenophora teres, forming a bacterial block at the edge of the bacterial colony by using a puncher with the diameter of 5mm, transferring the bacterial block to the center of a prepared toxic PDA culture medium by using an inoculation needle, and culturing in an incubator at 25 ℃ for 4 times. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was corrected. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:

Table 5: toxicity test results for Pyrenophora teres

As can be seen from Table 5, when the combination of isopyrazam and metiram is in the range of the mixture ratio of 10:1 to 1:50, the co-toxicity coefficients to Pyrenophora teres are all larger than 120, and the synergistic effect is shown.

Experiment six: indoor toxicity determination of isopyrazam and metiram on venturia inaequalis

Adopting a method for inhibiting the growth rate of hypha:

dissolving isopyrazam and metiram respectively with acetone, diluting with 0.1% Tween-80 aqueous solution to prepare liquid medicines with series concentrations, respectively sucking 6mL into sterilized triangular flasks in a super clean bench, adding 54mL of potato glucose agar (PDA) culture medium at about 50 ℃, shaking uniformly, pouring into 4 dishes with diameters of 9cm, and making into 4 toxic culture media with corresponding concentrations; the compound liquid medicine with different proportions of isopyrazam and metiram series concentration is prepared into a toxic culture medium by the same method. The Venturia inaequalis cultured for 2 days is beaten into bacterium blocks at the edges of the bacterial colonies by a puncher with the diameter of 5mm, the bacterium blocks are transferred to the center of a prepared toxic PDA culture medium by an inoculation needle, and then the obtained product is cultured in an incubator at 25 ℃ and the treatment is repeated for 4 times. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was determined by correction. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:

Table 6: toxicity test result for Venturia inaequalis

As can be seen from Table 6, when the combination of isopyrazam and metiram is in the range of the mixture ratio of 10:1 to 1:50, the number of co-toxicity coefficients to Venturia inaequalis more than 120, and a synergistic effect is shown.

Experiment seven: indoor toxicity determination of isopyrazam and metiram on wheat glume microspherical fungi

Adopting a method for inhibiting the growth rate of hypha:

dissolving isopyrazam and metiram respectively with acetone, diluting with 0.1% Tween-80 aqueous solution to prepare liquid medicines with series concentrations, respectively sucking 6mL into sterilized triangular flasks in a super clean bench, adding 54mL of potato glucose agar (PDA) culture medium at about 50 ℃, shaking uniformly, pouring into 4 dishes with diameters of 9cm, and making into 4 toxic culture media with corresponding concentrations; the compound liquid medicine with different proportions of isopyrazam and metiram series concentration is prepared into a toxic culture medium by the same method. The wheat glume small coccoid cultured for 2 days is beaten into bacterium blocks at the edges of the bacterium colony by a puncher with the diameter of 5mm, the bacterium blocks are moved to the center of a prepared toxic PDA culture medium by an inoculation needle, and then the bacterium blocks are placed in an incubator at 25 ℃ for culture, and the treatment is repeated for 4 times. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was determined by correction. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:

Table 7: toxicity test result for wheat glume small coccoid

As can be seen from Table 7, when the combination of isopyrazam and metiram is in the range of the mixture ratio of 10:1-1:50, the co-toxicity coefficient to wheat glume blight coccobacillus is greater than 120, and the synergistic effect is shown.

Experiment eight: indoor toxicity determination of dinieria parviflora by isopyrazam and metiram

Adopting a method for inhibiting the growth rate of hypha:

dissolving isopyrazam and metiram respectively with acetone, diluting with 0.1% Tween-80 aqueous solution to prepare liquid medicines with series concentrations, respectively sucking 6mL into sterilized triangular flasks in a super clean bench, adding 54mL of potato glucose agar (PDA) culture medium at about 50 ℃, shaking uniformly, pouring into 4 dishes with diameters of 9cm, and making into 4 toxic culture media with corresponding concentrations; the compound liquid medicine with different proportions of isopyrazam and metiram series concentration is prepared into a toxic culture medium by the same method. Pulverizing 2-day-cultured Pinctada yushuensis into blocks at the edge of colony with a punch with diameter of 5mm, transferring the blocks to the center of prepared toxic PDA culture medium with inoculating needle, culturing in 25 deg.C incubator, and repeating for 4 times. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was determined by correction. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:

Table 8: toxicity test result for Erysia peltata

From table 8, when the combination of isopyrazam and metiram is in the range of the mixture ratio of 10:1-1:50, the co-toxicity coefficient to the dahlia rosea is greater than 120, and the synergistic effect is shown.

Experiment nine: indoor toxicity determination of isopyrazam and metiram on pythium ultimum

Adopting a method for inhibiting the growth rate of hypha:

dissolving isopyrazam and metiram respectively with acetone, diluting with 0.1% Tween-80 aqueous solution to prepare liquid medicines with series concentrations, respectively sucking 6mL into sterilized triangular flasks in a super clean bench, adding 54mL of potato glucose agar (PDA) culture medium at about 50 ℃, shaking uniformly, pouring into 4 dishes with diameters of 9cm, and making into 4 toxic culture media with corresponding concentrations; the compound liquid medicine with different proportions of isopyrazam and metiram series concentration is prepared into a toxic culture medium by the same method. Pythium ultimum cultured for 2 days is punched into bacterial blocks at the edges of bacterial colonies by a puncher with the diameter of 5mm, the bacterial blocks are transferred to the center of a prepared toxic PDA culture medium by an inoculation needle, and then the bacterial blocks are placed in an incubator at 25 ℃ for culture, and each treatment is repeated for 4 times. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was corrected. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:

Table 9: toxicity test results for Pythium ultimum

As can be seen from Table 9, when the combination of isopyrazam and metiram is in the range of the mixture ratio of 10:1 to 1:50, the co-toxicity coefficient to pythium ultimum is greater than 120, and the synergistic effect is shown.

Experiment ten: indoor toxicity determination of isopyrazam and metiram on plasmopara viticola

Adopting a method for inhibiting the growth rate of hypha:

dissolving isopyrazam and metiram respectively with acetone, diluting with 0.1% Tween-80 aqueous solution to prepare liquid medicines with series concentrations, respectively sucking 6mL into sterilized triangular flasks in a super clean bench, adding 54mL of potato glucose agar (PDA) culture medium at about 50 ℃, shaking uniformly, pouring into 4 dishes with diameters of 9cm, and making into 4 toxic culture media with corresponding concentrations; the compound liquid medicine with different proportions of isopyrazam and metiram series concentration is prepared into a toxic culture medium by the same method. The 2-day-old Plasmopara viticola was inoculated into a block at the edge of a colony using a punch having a diameter of 5mm, the block was transferred to the center of a previously prepared PDA medium containing a toxin using an inoculating needle, and the block was cultured in an incubator at 25 ℃ 4 times per treatment. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was determined by correction. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:

Table 10: toxicity test results on Plasmopara viticola

As can be seen from Table 10, when the combination of isopyrazam and metiram is in the range of the mixture ratio of 10:1 to 1:50, the co-toxicity coefficient to Plasmopara viticola is more than 120, and the synergistic effect is shown.

Test eleven: toxicity assay for barley powdery mildew

Adopting a method for inhibiting the growth rate of hypha:

dissolving isopyrazam and metiram respectively with acetone, diluting with 0.1% Tween-80 aqueous solution to prepare liquid medicines with series concentrations, respectively sucking 6mL into sterilized triangular flasks in a super clean bench, adding 54mL of potato glucose agar (PDA) culture medium at about 50 ℃, shaking uniformly, pouring into 4 dishes with diameters of 9cm, and making into 4 toxic culture media with corresponding concentrations; the compound liquid medicine with different proportions of isopyrazam and metiram series concentration is prepared into a toxic culture medium by the same method. The barley powdery mildew which is cultured for 2 days is beaten into fungus blocks at the edges of the colonies by a puncher with the diameter of 5mm, the fungus blocks are transferred to the center of a prepared toxic PDA culture medium by an inoculation needle, and then the barley powdery mildew is cultured in an incubator at 25 ℃ and each treatment is repeated for 4 times. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was determined by correction. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:

Table 11: toxicity test result for barley powdery mildew

As can be seen from Table 11, when the combination of isopyrazam and metiram is in the range of the mixture ratio of 10:1 to 1:50, the co-toxicity coefficient to barley powdery mildew is more than 120, and the synergistic effect is shown.

Test twelve: toxicity determination of puccinia recondita

Adopting a method for inhibiting the growth rate of hypha:

dissolving isopyrazam and metiram respectively with acetone, diluting with 0.1% Tween-80 aqueous solution to prepare liquid medicines with series concentrations, respectively sucking 6mL into sterilized triangular flasks in a super clean bench, adding 54mL of potato glucose agar (PDA) culture medium at about 50 ℃, shaking uniformly, pouring into 4 dishes with diameters of 9cm, and making into 4 toxic culture media with corresponding concentrations; the compound liquid medicine with different proportions of isopyrazam and metiram series concentration is prepared into a toxic culture medium by the same method. The puccinia recondita of wheat cultured for 2 days is punched into blocks at the edge of the colony by a puncher with the diameter of 5mm, the blocks are transferred to the center of a prepared toxic PDA culture medium by an inoculation needle, and then the blocks are cultured in an incubator at 25 ℃ and the treatment is repeated for 4 times. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was determined by correction. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:

Table 12: toxicity test result for puccinia recondita of wheat

As can be seen from table 12, when the combination of isopyrazam and metiram is in the range of the ratio of 10:1 to 1:50, the co-toxicity coefficient to puccinia recondita is greater than 120, showing a synergistic effect.

Test thirteen: virulence determination of alternaria solani

Adopting a method for inhibiting the growth rate of hypha:

dissolving isopyrazam and metiram respectively with acetone, diluting with 0.1% Tween-80 aqueous solution to prepare liquid medicines with series concentrations, respectively sucking 6mL into sterilized triangular flasks in a super clean bench, adding 54mL of potato glucose agar (PDA) culture medium at about 50 ℃, shaking uniformly, pouring into 4 dishes with diameters of 9cm, and making into 4 toxic culture media with corresponding concentrations; the compound liquid medicine with different proportions of isopyrazam and metiram series concentration is prepared into a toxic culture medium by the same method. The alternaria solani cultured for 2 days is beaten into bacterial blocks at the edges of bacterial colonies by a puncher with the diameter of 5mm, the bacterial blocks are transferred to the center of a prepared toxic PDA culture medium by an inoculation needle, and then the bacterial blocks are cultured in an incubator at 25 ℃, and the treatment is repeated for 4 times. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was determined by correction. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:

Table 13: results of virulence test on alternaria solani

As can be seen from Table 13, when the combination of isopyrazam and metiram is in the range of the mixture ratio of 10:1 to 1:50, the co-toxicity coefficient to Alternaria solani is greater than 120, and the synergistic effect is shown.

Fourteen experiments: toxicity determination of leptospirillum viticola

Adopting a method for inhibiting the growth rate of hypha:

dissolving isopyrazam and metiram respectively with acetone, diluting with 0.1% Tween-80 aqueous solution to prepare liquid medicines with series concentrations, respectively sucking 6mL into sterilized triangular flasks in a super clean bench, adding 54mL of potato glucose agar (PDA) culture medium at about 50 ℃, shaking uniformly, pouring into 4 dishes with diameters of 9cm, and making into 4 toxic culture media with corresponding concentrations; the compound liquid medicine with different proportions of isopyrazam and metiram series concentration is prepared into a toxic culture medium by the same method. Culturing 2 days old Uncaria rhynchophylla, punching into bacterial blocks at the edges of bacterial colony with a punch with diameter of 5mm, transferring the bacterial blocks to the center of a prepared toxic PDA culture medium with an inoculating needle, culturing in an incubator at 25 deg.C, and repeating for 4 times each treatment. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was determined by correction. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:

Table 14: toxicity test result for leptospirillum vitis

As can be seen from table 14, when the combination of isopyrazam and metiram is in the range of the ratio of 10:1 to 1:50, the co-toxicity coefficient to leptospirillum viticola is greater than 120, and the synergistic effect is shown.

Test fifteen: toxicity determination of cucurbits erysiphe necator

Adopting a method for inhibiting the growth rate of hypha:

dissolving isopyrazam and metiram respectively with acetone, diluting with 0.1% Tween-80 aqueous solution to prepare liquid medicines with series concentrations, respectively sucking 6mL into sterilized triangular flasks in a super clean bench, adding 54mL of potato glucose agar (PDA) culture medium at about 50 ℃, shaking uniformly, pouring into 4 dishes with diameters of 9cm, and making into 4 toxic culture media with corresponding concentrations; the compound liquid medicine with different proportions of isopyrazam and metiram series concentration is prepared into a toxic culture medium by the same method. Culturing for 2 days, beating the melon pericarp powdery mildew with a punch with diameter of 5mm into blocks at the edge of the colony, transferring the blocks to the center of a prepared toxic PDA culture medium with an inoculating needle, culturing in an incubator at 25 deg.C, and repeating for 4 times each treatment. After 3 days, the diameter cm of each treated colony was measured with a caliper by the cross method, and the percent inhibition was determined by correction. Two diameters were cross-measured per colony, and the average was used to represent colony size. Then, the colony growth inhibition rate was determined according to the following formula:

Table 15: toxicity test result for cucurbits erysiphe cichoracearum

As can be seen from Table 15, when the combination of isopyrazam and metiram is in the range of the mixture ratio of 10:1 to 1:50, the co-toxicity coefficient to the cucurbit erysiphe cichoracearum is more than 120, and the synergistic effect is shown.

Claims

1. A germicidal composition, characterized by: the bactericidal composition contains active ingredients of isopyrazam and metiram, wherein the weight percentage of the isopyrazam to the metiram is 10:1-1:50, more preferably 5:1-1:40, still more preferably 1:1-1:30, more preferably 1:1-1:25, more preferably 1:1-1:20, more preferably 1:1-1:15, more preferably 1:5-1:12, and more preferably 1:8-1: 12.

2. The germicidal composition of claim 1, wherein: the sum of the mass of the isopyrazam and the mass of the metiram accounts for 5-90%, preferably 10-80% and more preferably 20-70% of the mass of the sterilization composition.

3. The germicidal composition of claim 1, wherein: the dosage form of the bactericidal composition is capsule suspending agent, capsule granule, fine granule, large granule, micro granule, oil dispersible powder, suspending agent, seed coating agent, wettable powder, water dispersible granule, soluble powder, microcapsule suspending agent, coated granule, extruded granule, missible oil, microemulsion, emulsion in water, effervescent tablet, ultra-low volume liquid, suspoemulsion, ultra-low volume cold atomization preparation, ultra-low volume hot atomization preparation, couple package (twinpack), seed treatment dry powder, seed treatment emulsion, seed treatment suspending agent, seed treatment liquid, seed treatment dispersible powder, seed treatment microcapsule suspending agent, seed treatment gel, suspoemulsion and emulsion granule.

4. The germicidal composition of claim 1, wherein: also comprises a filler and/or a surfactant.

5. A method of controlling or preventing infestation of cultivated plants by phytopathogenic fungi, characterized in that: the fungicidal composition of claim 1 applied to a plant pathogen and/or its environment, or to a plant, plant propagation material and plant organs, soil or cultivation media, materials or spaces which grow at a later time.

6. A method of controlling or preventing infestation of cultivated plants by phytopathogenic fungi, characterized in that: the isopyrazam and metiram of claim 1 are administered simultaneously or separately.

7. A method of controlling or preventing infestation of cultivated plants by phytopathogenic fungi, characterized in that: the fungicidal composition according to claim 1 is applied to the phytopathogenic fungi and/or their environment, or to plants, plant propagation material and plant organs, soil or cultivation media, materials or spaces which grow out later, before or after the infestation of the plants.

8. A method of controlling or preventing infestation of cultivated plants by phytopathogenic fungi, characterized in that: the fungicidal composition of claim 1 is applied to the phytopathogenic fungi and/or their environment, or to plants, plant propagation material and subsequently growing plant organs, soil or cultivation media, materials or spaces in an agronomically effective and substantially non-phytotoxic application rate by seed treatment, foliar application, stem application, drenching, instillation, pouring, spraying, misting, dusting, scattering or fuming.

9. A method of controlling or preventing infestation of cultivated plants by phytopathogenic fungi, characterized in that: comprising applying the fungicidal composition of claim 1 to foliage of a plant.

10. A method of controlling or preventing infestation of cultivated plants by phytopathogenic fungi, characterized in that: comprising applying the fungicidal composition of claim 1 to plant propagation material and subsequently emerging plant organs.

11. A method of controlling or preventing infestation of cultivated plants by phytopathogenic fungi, characterized in that: comprising applying the fungicidal composition of claim 1 to soil or a cultivation medium.

12. Use of the fungicidal composition according to claim 1 for controlling fungi and bacteria on cereals, vegetables, fruits, ornamentals and vines.

13. Use of the fungicidal composition according to claim 1 for controlling harmful fungi of the genera Anabaena, Podosphaera, Erysiphe, Candida, Mycosphaera, Sphaerotheca, Puccinia, Rhizoctonia, Puccinia, Tinospora, Tilletia, Botrytis, Helminthosporium, Trichosporon, Fusarium, Septoria, Cercospora, Alternaria, Pseudocercospora, Phytophthora, Peronospora, Pseudoperonospora, Puccinia, Aureobasidium, Pythium, Plasmopara on graminum.

14. Use of the fungicidal composition of claim 1 for controlling wheat leaf rust, apple tip powdery mildew, apple scab, barley powdery mildew, grape gray mold, tomato gray mold, wheat leaf blight, barley net blotch, tomato early blight, grape powdery mildew, barley powdery mildew, wheat tip head rot, wheat take-all, wheat leaf rust, rice sheath blight, wheat glume blight, wheat leaf blight, rice blast, potato early blight, potato late blight, cucumber damping-off, maize smut, eye spot, grape downy mildew, bean gray mold, wheat brown rust, apple powdery mildew, grape powdery mildew, cucumber powdery mildew, cereal eye spot.

15. Use of the fungicidal composition according to claim 1 for the protection of plants, plant propagation material and plant organs that grow at a later time.

16. Use of the fungicidal composition of claim 1 for controlling pathogenic or saprophytic fungi and bacteria in soil or cultivation media, applied to the locus where control is desired.

17. Use of the fungicidal composition according to claim 1 for the treatment of seeds to protect the seeds from attack by carried phytopathogens.

18. Use of the fungicidal composition of claim 1 for the protection of stored goods.

19. Use of the fungicidal composition of claim 1 for protecting stored products from fungal or bacterial infestation during storage.