CN111781197A - Sepsis rapid detection kit and process - Google Patents

Abstract

The invention discloses a rapid sepsis detection kit which can realize multi-group detection and can avoid the influence of external environment on a detection result. The sepsis rapid detection kit comprises a box body, wherein vertical detection cavities which are uniformly distributed along the transverse direction are arranged on the box body; a discharge channel communicated with the transverse main channel is arranged on the vertical detection cavity; one end of the main channel is provided with a micro fan, and the other end of the main channel is provided with a micro circulating pump; a cleaning solution storage tube, a diluent storage tube, an injector and a dilution test tube are arranged in the box body; the box body is provided with a detection reagent injector and a detection probe. Adopt this sepsis rapid detection kit to include the box body, can realize that the multiunit detects, be convenient for improve and detect the accuracy, can realize the washing to detecting the chamber, be convenient for realize used repeatedly.

Description

Sepsis rapid detection kit and process

technical field

The invention relates to the field of disease detection, in particular to a sepsis rapid detection kit.

Background technique

Well-known: sepsis refers to the systemic inflammatory response syndrome systemic inflammatory response syndrome, SIRS caused by infection, clinically confirmed to have bacteria or highly suspicious infection foci. Although sepsis is caused by infection, once it occurs, its occurrence and development follow its own pathological process and laws, so in essence, sepsis is the body's response to infectious factors.

There are multiple ways to detect sepsis, such as kits, methods and devices for analyzing biological samples from animals to predict (before symptoms appear) and monitor the development of sepsis disclosed in Chinese patent application 201580013411.7. Utilize biomarker features, especially those that can provide an average predictive accuracy of at least 92% to distinguish between sepsis and non-sepsis development. Although this method has high detection accuracy, it is all completed in the laboratory, so the operation is complicated and time-consuming, and the efficiency is low.

Another detection method, such as a kit for rapid detection of sepsis disclosed in Chinese Patent Application No. 201910547140.3, includes a plastic box body, and a pull-out plate is slidably connected to the inside of the plastic box body, and the front surface of the pull-out plate is The wall is provided with a handle, and the pull-out plate is provided with an installation slot, a first slot and a second slot, the installation slot is located at one end of the first slot and the second slot, and the second slot is located at the first slot. One side of a card slot, a chip slot and an external temperature detector are arranged inside the installation slot, two symmetrical airbags are arranged on the bottom side plate of the chip box, and the bottoms of the two airbags are pasted on the bottom side On the board, a plane that can place the chip box and the processor is formed, and the external temperature detector is convenient to detect the indoor temperature and display it through the display. It is convenient for users to watch the indoor temperature, and prevent the indoor temperature from being too high or too low, which affects the accuracy of the kit detection.

Although this detection method can overcome the influence of the external environment on the detection results, it can only perform a single monitoring, and cannot form an effective comparative detection, so that the accuracy of the detection results cannot be guaranteed.

SUMMARY OF THE INVENTION

The technical problem to be solved by the present invention is to provide a sepsis rapid detection kit that can realize multi-group detection and can avoid the external environment from affecting the detection results.

The technical solution adopted by the present invention to solve the technical problem is as follows: a sepsis rapid detection kit includes a box body, and the box body is provided with vertical detection chambers uniformly distributed along the lateral direction; the upper end of the vertical detection chamber is provided with With rubber plug;

The lower end of the vertical detection cavity is provided with a discharge channel; the discharge channel is provided with a regulating valve;

The middle of the box body is provided with a transverse main channel running through both sides of the box body; one end of the main channel is provided with a micro fan, and the other end is provided with a micro circulation pump; the discharge channel is communicated with the transverse main channel; A battery is arranged; a second valve is arranged between the micro-fan and the main channel; a first valve is arranged between the micro-circulation pump and the main channel;

The lower end of the box body is provided with a first storage circular cavity with an opening on one side of the box body; the end of the first storage circular cavity with an opening is provided with a first plug board; the first storage circular cavity There are at least two; the first plug-in board is inserted into the box to seal the first storage circular cavity;

The lower end of the box body is provided with a second storage circular cavity and a third storage circular cavity with openings on the other side of the box body; one end of the second storage circular cavity shown is provided with a second plug board; The plug-in board is inserted into the box to seal the second storage circular cavity and the third storage circular cavity;

The middle position of the lower end of the box body is provided with a vertical circular storage cavity; the lower end of the vertical circular storage cavity is provided with a third plug board; the third plug board is inserted into the box body to seal the vertical circular storage cavity cavity;

The first storage circular cavity is provided with a cleaning liquid storage tube; the vertical storage circular cavity is provided with a diluent storage tube; the second storage circular cavity is provided with a syringe and the third storage A dilution test tube is arranged in the circular cavity;

The back of the box body is provided with a processor and a detection probe inserted into the vertical detection cavity and used to detect temperature and pressure; the detection probe is electrically connected to the processor;

The front side of the box body is provided with a transparent observation window for observing the vertical detection cavity; one side of the transparent observation window is provided with a colorimetric card;

The front of the box body is provided with a detection reagent injector inserted into the upper part of the vertical detection cavity; one side of the vertical detection cavity in the box body is provided with a small heating resistor; the front of the box body is provided with a touch display screen;

The micro-fan, the micro-circulating pump, the small heating resistance, the touch display screen and the detection probe are respectively electrically connected with the processor;

The storage battery provides electric power for the micro fan, the micro circulation pump, the small heating resistance, the touch display screen, the detection probe and the processor.

Specifically, the detection reagent injector adopts a micro-syringe; the detection reagent injector has a tube body, a push head, an injection needle and a connecting head; the connecting head is screwed with the box body.

Specifically, the detection probe is provided with an induction probe; the induction probe is threadedly matched with the box body;

The induction probe is provided with a temperature sensor and a pressure sensor; the temperature sensor and the pressure sensor are located in a vertical detection cavity; a detection chip is arranged at the front end of the induction probe.

Specifically, one side of the box body is provided with a charging interface for the battery.

Specifically, the front side of the box body is provided with transparent observation windows for the first storage circular cavity, the vertical storage circular cavity, the second storage circular cavity and the third storage circular cavity.

The beneficial effects of the present invention are as follows: the sepsis rapid detection kit of the present invention is provided with multiple detection reagent chambers, so multiple groups of detections can be realized at the same time, and the detection results can be compared, thereby ensuring and improving the detection efficiency. accuracy.

Secondly, since a micro-fan and a reagent injector in the form of a syringe are provided, the air pressure in the detection chamber can be adjusted through the micro-fan and the reagent injector; at the same time, the temperature in the detection chamber can be detected by a temperature sensor, and a heating resistor is provided , so as to facilitate the adjustment of the reaction temperature. Therefore, it can be seen that the external environment has an influence on the detection result.

Thirdly, by arranging the cleaning liquid and the circulating pump, the detection chamber can be cleaned, which is convenient for repeated use.

Description of drawings

Fig. 1 is the perspective view of sepsis rapid detection kit in the embodiment of the present invention;

Fig. 2 is the front view of the sepsis rapid detection kit in the embodiment of the present invention;

Fig. 3 is the rear view of the sepsis rapid detection kit in the embodiment of the present invention;

Fig. 4 is the top view of the sepsis rapid detection kit in the embodiment of the present invention;

Fig. 5 is A-A sectional view in Fig. 4;

6 is a perspective view of a detection reagent injector in an embodiment of the present invention;

7 is a schematic structural diagram of a detection reagent injector in an embodiment of the present invention;

FIG. 8 is a perspective view of a detection probe in an embodiment of the present invention.

Detailed ways

The present invention will be further described below in conjunction with the accompanying drawings and embodiments.

As shown in FIG. 1 to FIG. 8 , the sepsis rapid detection kit according to the present invention includes a box body 1, and the box body 1 is provided with vertical detection chambers 2 uniformly distributed in the lateral direction; The upper end of the detection chamber 2 is provided with a rubber plug 7;

The lower end of the vertical detection chamber 2 is provided with a discharge channel 3; the discharge channel 3 is provided with a regulating valve 4;

The middle position of the box body 1 is provided with a transverse main channel 8 running through both sides of the box body 1; one end of the main channel 8 is provided with a micro fan 13, and the other end is provided with a micro circulation pump 11; 8 connected;

A battery 15 is arranged in the box body 1; a second valve 10 is arranged between the micro-fan 13 and the main channel 8; a first valve 9 is arranged between the micro-circulation pump 11 and the main channel 8;

The lower end of the box body 1 is provided with a first storage circular cavity 17 with an opening on one side of the box body 1; A storage circular cavity 17 has at least two; the first plug-in board 18 is inserted into the box body 1 to seal the first storage circular cavity 17;

The lower end of the box body 1 is provided with a second storage circular cavity 24 and a third storage circular cavity 23 with openings on the other side of the box body 1; one end of the second storage circular cavity 24 shown is provided with a second plug board 26; the second plug board 26 is inserted into the box body 1 to seal the second storage circular cavity 24 and the third storage circular cavity 23;

The middle position of the lower end of the box body 1 is provided with a vertical circular storage cavity 20; the lower end of the vertical circular storage cavity 20 is provided with a third plug board 21; the third plug board 21 is inserted into the box body 1. A vertical storage circular cavity 20 is sealed inside;

The first storage circular cavity 17 is provided with a cleaning liquid storage tube 19; the vertical storage circular cavity 20 is provided with a diluent storage tube 22; the second storage circular cavity 24 is provided with a syringe 27 And the third storage circular cavity 23 is provided with a dilution test tube 25;

The back of the box body 1 is provided with a processor 29 and a detection probe 5 inserted into the vertical detection cavity 2 and used to detect temperature and pressure; the detection probe 5 is electrically connected to the processor 29;

The front of the box body 1 is provided with a transparent observation window 31 for observing the vertical detection cavity 2; one side of the transparent observation window 31 is provided with a colorimetric card 30;

The front of the box body 1 is provided with a detection reagent injector 6 inserted into the upper part of the vertical detection cavity 2; the side of the vertical detection cavity 2 in the box body 1 is provided with a small heating resistor 33; A touch screen 32 is provided;

The micro-fan 13, the micro-circulation pump 11, the small heating resistor 33, the touch screen 32 and the detection probe 5 are respectively electrically connected to the processor 29;

The battery 15 provides electrical energy for the micro fan 13 , the micro circulation pump 11 , the small heating resistor 33 , the touch screen 32 , the detection probe 5 and the processor 29 .

Specifically, in the application process, first open the regulating valve 4 and the second valve 10, remove the rubber plug 7; make the vertical detection chamber 2 communicate with the micro-fan 13; then start the micro-fan 13 to make the micro-fan pump air, Make the vertical detection chamber 2 and the pipeline cool, and judge whether the temperature at this time is lower than the detection reaction temperature through the temperature displayed on the touch screen 32; if the temperature is lower than the detection reaction temperature, the micro-fan 13 is braked, and then the regulating valve 4 and the first temperature are closed. Valve 10.

Then remove the syringe 27, the dilution test tube 25 and the diluent storage tube 22 to dilute the blood sample; then inject the diluted blood sample into the vertical detection chamber 2; and plug the rubber plug 7; then pass the detection reagent The injector 6 injects all the detection reagents into the vertical detection chamber 2; at this time, by touching the air pressure displayed on the display screen 32, it is judged whether the air pressure meets the detection air pressure. Since the vertical detection chamber 2 is in a sealed state when the detection reagent is injected, At this time, the gas pressure of the injected detection reagent will increase, and part of the gas in the vertical detection chamber 2 is drawn out through the detection reagent injector 6, thereby reducing the gas pressure to meet the requirements of the detection reaction.

Then, by adding a small resistor 33, the vertical detection chamber 2 is heated, so that the temperature rise meets the detection requirements. At this time, the blood sample reacts with the detection reagent, and the reacted color is observed through the transparent observation window 31, and then compared with the colorimetric card 30, and sepsis is detected according to the color change, so as to realize the detection. After the detection is completed, the results of multiple groups of detections are compared and analyzed to obtain the final monitoring results.

After the detection is completed, the regulating valve 4 and the first valve 9 are opened and the rubber plug 7 is removed; the circulating pump 11 is started to extract the detected blood sample.

Then, take out the cleaning liquid storage tube 19; and block the vertical detection cavity 2 with the rubber plug 7, remove the rubber plug 7 on a vertical detection cavity 2; and then send the cleaning liquid into the vertical detection cavity 2; and the circulating pump 11 is communicated with the upper part of the vertical detection chamber 2 through a conduit, and the vertical detection chamber 2 is circulated and cleaned by the cleaning liquid. After the cleaning is completed, remove the catheter and discharge the cleaning liquid; then plug the rubber plug 7 on the vertical detection chamber 2, remove the rubber plug 7 of the other vertical detection chamber 2, repeat the above steps, and so on. , and finally complete the initial cleaning of the vertical detection chamber 2, and finally add a new cleaning solution to perform the second cleaning to complete the cleaning of the detection chamber.

After cleaning, remove the rubber plug 7, open the second valve 10, close the first valve 9, start the micro fan 13, and dry the detection chamber through the micro fan 13; then it can be used for the next detection.

To sum up, the sepsis rapid detection kit of the present invention is provided with multiple detection reagent chambers, so multiple groups of detections can be realized at the same time, and the detection results can be compared, thereby ensuring and improving the detection accuracy .

Secondly, since a micro-fan and a reagent injector in the form of a syringe are provided, the air pressure in the detection chamber can be adjusted through the micro-fan and the reagent injector; at the same time, the temperature in the detection chamber can be detected by a temperature sensor, and a heating resistor is provided , so as to facilitate the adjustment of the reaction temperature. Therefore, it can be seen that the external environment has an influence on the detection result.

Thirdly, by arranging the cleaning liquid and the circulating pump, the detection chamber can be cleaned, which is convenient for repeated use.

In order to facilitate installation, ensure the airtightness of the vertical detection cavity 2, and facilitate the adjustment of the air pressure in the vertical detection cavity 2, further, the detection reagent injector 6 adopts a micro-syringe; the detection reagent injector 6 has The pipe body 61 , the push head 62 , the injection needle 63 and the connecting head 64 ; the connecting head 64 is screwed with the box body 1 .

In order to facilitate installation and ensure the airtightness of the vertical detection cavity 2, the detection probe 5 is provided with an induction probe 51; the induction probe 51 is screwed with the box body 1;

The sensing probe 51 is provided with a temperature sensor 52 and a pressure sensor 53; the temperature sensor 52 and the pressure sensor 53 are located in the vertical detection cavity 2; the sensing probe 51 is provided with a detection chip 54 at the front end.

The detection chip 54 can integrate a pH value detection sensor and a corresponding ion detection sensor, so as to facilitate a more comprehensive analysis of the detection results.

In order to facilitate charging, preferably, a charging port 16 for the battery 15 is provided on one side of the box body 1 .

In order to facilitate the observation of the status of items stored in each storage cavity, further the front of the box body is provided with a first storage circular cavity, a vertical storage circular cavity, a second storage circular cavity and a third storage circular cavity transparent viewing window.

Claims (5)

1. Sepsis rapid detection kit, characterized in that: it comprises a box body (1), and the box body (1) is provided with a vertical detection cavity (2) uniformly distributed along the lateral direction; the vertical detection cavity (2) The upper end is provided with a rubber plug (7); A discharge channel (3) is arranged at the lower end of the vertical detection chamber (2); a regulating valve (4) is arranged on the discharge channel (3); The box body (1) is provided with a transverse main channel (8) running through both sides of the box body (1) in the middle position; one end of the main channel (8) is provided with a micro fan (13), and the other end is provided with a micro circulation pump (11); the discharge channel (3) communicates with the transverse main channel (8); a battery (15) is arranged in the box body (1); the micro-fan (13) and the main channel (8) are arranged between A second valve (10) is provided; a first valve (9) is provided between the micro-circulation pump (11) and the main channel (8); The lower end of the box body (1) is provided with a first storage circular cavity (17) with an opening on one side of the box body (1); a plug-in board (18); the first storage circular cavity (17) has at least two; the first plug-in board (18) is inserted into the box body (1) to seal the first storage circular cavity (17); The lower end of the box body (1) is provided with a second storage circular cavity (24) and a third storage circular cavity (23) with openings on the other side of the box body (1); the second storage circular cavity is shown (24) A second plug board (26) is provided at one end; the second plug board (26) is inserted into the box body (1) to seal the second storage circular cavity (24) and the third storage circular cavity (23) ; A vertical circular storage cavity (20) is provided at the middle position of the lower end of the box body (1); a third plug board (21) is provided at the lower end of the vertical circular storage cavity (20); The third plug board (21) is inserted into the box body (1) to seal the vertical circular storage cavity (20); The first storage circular cavity (17) is provided with a cleaning liquid storage tube (19); the vertical storage circular cavity (20) is provided with a dilution liquid storage tube (22); the second storage circular A syringe (27) is arranged in the cavity (24) and a dilution test tube (25) is arranged in the third storage circular cavity (23); The back of the box body (1) is provided with a processor (29) and a detection probe (5) inserted into the vertical detection cavity (2) and used to detect temperature and pressure; the detection probe (5) and the processing The device (29) is electrically connected; A transparent observation window (31) for observing the vertical detection cavity (2) is provided on the front of the box body (1); a colorimetric card (30) is provided on one side of the transparent observation window (31); The front of the box body (1) is provided with a detection reagent injector (6) inserted into the upper part of the vertical detection cavity (2); a heating device is provided on one side of the vertical detection cavity (2) in the box body (1). A small resistor (33); a touch display screen (32) is provided on the front of the box body (1); the micro-fan (13), the micro-circulation pump (11), the small heating resistor (33), the touch screen (32) and the detection probe (5) are respectively electrically connected to the processor (29); The storage battery (15) provides electric power for the micro fan (13), the micro circulation pump (11), the small heating resistor (33), the touch display screen (32), the detection probe (5) and the processor (29). 2. The sepsis rapid detection kit according to claim 1, characterized in that: the detection reagent injector (6) adopts a micro-syringe; the detection reagent injector (6) has a tube body (61), A push head (62), an injection needle (63) and a connecting head (64); the connecting head (64) is screwed with the box body (1). 3. The sepsis rapid detection kit according to claim 2, characterized in that: the detection probe (5) is provided with an induction probe (51); the induction probe (51) and the box body (1) thread fit; The sensing probe (51) is provided with a temperature sensor (52) and a pressure sensor (53); the temperature sensor (52) and the pressure sensor (53) are located in the vertical detection cavity (2); the sensing probe ( 51) The front end is provided with a detection chip (54). 4. The sepsis rapid detection kit according to claim 2, characterized in that: one side of the box body (1) is provided with a charging port (16) for the battery (15). 5. The sepsis rapid detection kit according to claim 4, characterized in that: the front of the box body (1) is provided with a first storage circular cavity (17), a vertical storage circular cavity (20) ), the second storage circular cavity (24) and the transparent viewing window of the third storage circular cavity (23).

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