CN111701718A - A kind of granular fat screening and purification device and method - Google Patents

Abstract

The invention belongs to the technical field of medicine, and in particular relates to a particle fat screening and purification device and method. The device includes an upper tank body, a ring-shaped intermediate connecting piece and a lower tank body. The upper and lower tank bodies are respectively provided with pipeline interfaces, and the upper and lower tank bodies are respectively detachably connected with the intermediate connecting piece. A jet ring is disassembled and connected, a screen is arranged above the jet ring, the screen is detachably connected with the intermediate connecting piece, and a middle pipe interface leading to the jetting ring is also arranged on the outer surface of the intermediate connecting piece. The device further comprises a bracket, the bracket and the intermediate connecting piece are connected horizontally for rotation, and the bracket is provided with a locking mechanism for restricting the rotation of the intermediate connecting piece. The invention uses the buoyancy principle to sieve and collect the granular fat, does not use direct mechanical force, adopts shaking and jet flow to separate the fat particles and ensures the dredging of the screen, and can efficiently screen the fat particles of the specified size.

Description

A kind of granular fat screening and purification device and method

technical field

The invention relates to techniques of fat transplantation, adipose tissue component extraction, adipose stem cell extraction and the like, belonging to the technical fields of tissue engineering, regenerative medicine and clinical medicine, and in particular to a particle fat screening and purification device and method.

Background technique

Tissue defects caused by trauma, tumors, congenital deformities and aging are the focus of plastic surgery work. Autologous fat particle transplantation is an important technical means currently used in tissue regeneration and repair and medical beauty, and has been more and more widely used in clinical practice. . However, the effect of fat grafts is still very limited at present, and problems such as absorption and necrosis in fat grafting are still unavoidable. The main reasons are: first, the fat purification method commonly used in fat transplantation has low efficiency, large damage and poor effect; second, the size of the optimal fat particles for different parts and amounts of fat filling is different. There is still a lack of effective methods to separate fat particles of different sizes.

The purification of granular fat is a very critical process in fat transplantation. Through this process, a large amount of water, oil, blood components, fibrous strands and other harmful components that are not conducive to the survival of adipose tissue can be removed from the granular fat graft. At present, the commonly used methods for the purification of granular fat in the world include static method, centrifugation method, cotton pad method, etc. A large number of studies have confirmed that the transplantation effect of granular fat graft obtained by cotton pad method is obviously better than centrifugal method and static method. However, due to adsorption, extrusion, etc., the loss of fat mass caused by the cotton pad method is also the largest. So far, no fat purification method has been found that can not only damage fat but also effectively remove impurities.

The size of the fat particles is another important factor for survival after transplantation. After a large number of studies, it is believed that fat cells within 2mm of the vascularized tissue can survive through the mechanism of plasma absorption and early revascularization. Yoshi imura proposed the "three-zone" concept of transplantation substitution theory, and believes that the fat graft within 300um of the periphery is transplanted. Adipocytes can survive, and beyond 300um from the inside to the outside are the regeneration area and the necrosis area, and Eto et al. further proved that the adipose tissue in the latter two areas is mainly differentiated and regenerated from activated adipose stem cells. Therefore, large-diameter granular fat may develop marginal necrosis after transplantation resulting in low fat graft volume retention. However, when large-volume fat is used to fill defects in clinical practice, the effect of granular fat grafting obtained with smaller diameter liposuction needles is not good, and the incidence of complications increases significantly. In our previous experiments, it was found that obvious lipid droplets were generated when screening fat granules of 0.5 mm and below, which indicated that small-diameter fat granules were less mechanically resistant and more prone to rupture when processing adipose tissue. Therefore, we believe that the appropriate diameter of granular fat can not only improve the survival rate of fat grafts, but also reduce the loss of granular fat.

The existing granular fat filtration devices on the market are all mechanical pressure types. By applying positive or negative pressure to force the granular fat to pass through the screen, there are serious deficiencies: first, the pressure itself causes great damage to fat cells; second, Granular fat is forcibly squeezed through the screen and is subjected to huge shearing force, which destroys the integrity and vitality of fat cells; third, larger particles quickly block the screen, a large number of small particles cannot pass through the screen holes, and the efficiency is extremely low; Fourth, the use of mechanical scraping to clear the screen further aggravates the destruction of fat cells.

In conclusion, how to effectively remove impurities in lipoaspirate and effectively screen fat particles of the required size is a key issue to improve the survival and efficiency of fat transplantation. The existing technology and equipment cannot obtain fat particles with a set diameter and a uniform size under the premise of lossless and high efficiency. It is necessary to provide a new particle fat screening and purification device and method to solve the deficiencies of the existing technology.

SUMMARY OF THE INVENTION

In order to solve the deficiencies of the prior art, the present invention provides a particle fat screening and purification device and method. The outstanding features of the particle fat screening and purification device and method of the present invention are non-destructive and high-efficiency: sieving and collection are carried out using the principle of buoyancy, no direct mechanical force is used, and the fat particles are separated by shaking and jet flow, and the dredging of the screen can be ensured. Efficient screening of fat particles of specified size. Its advantages are: firstly, it greatly protects the structure of fat particles and reduces the damage to fat cells; secondly, the obtained fat particles are of uniform size, precise and controllable; thirdly, it improves filtration efficiency and reduces waste and loss; Fourth, the whole process of airtight aseptic operation reduces the risk of contamination; fifth, the process can be repeated to obtain fat particles of various sizes.

The technical scheme provided by the present invention is as follows:

A particle fat screening and purification device, comprising an upper tank body, a ring-shaped intermediate connecting piece and a lower tank body which are connected up and down in sequence, the upper part of the upper tank body is provided with an upper pipe interface, and the lower part of the lower tank body is provided with There is a lower pipe interface, the lower part of the upper tank body is detachably connected with the upper part of the intermediate connecting piece, and the upper part of the lower tank body is detachably sealingly connected with the lower part of the intermediate connecting piece. A jet ring is detachably connected to the inner ring of the intermediate connecting piece, a screen is arranged above the jetting ring, the screen is detachably connected to the intermediate connecting piece, and the outer surface of the intermediate connecting piece is also detachably connected. A middle pipe interface leading to the jet ring is provided, and the particle fat screening and purification device further comprises a bracket, the bracket is connected with the intermediate connecting piece in a horizontal rotation, and the bracket is provided with a limiter of the intermediate connecting piece. A locking mechanism that rotates about its horizontal axis every half-turn.

The particle fat screening and purification device provided by the above technical solution utilizes the physical principle that the screen only allows objects smaller than the aperture size to pass through to filter and separate fat particles of different sizes; For filtration, first use a screen with a larger aperture, and then use a screen with a smaller aperture, so as to obtain fat particles with any size between the two apertures, and at the same time automatically filter out the grease smaller than the two apertures; use the adipose tissue and the specific gravity of the oil It is lower than water, and the principle of buoyancy is applied to make the fat particles automatically float in the liquid and pass through the screen, and concentrate on the uppermost layer of the liquid.

Specifically, the jet ring includes a ring-shaped body, and the body is provided with a plurality of guide grooves on the upper end face of the body in the circumferential direction, which communicate with the inner wall and the outer wall; The normal angle of the outer wall of the body is 0-45°.

Further, the body includes an upper ring part and a lower ring part that are fixedly connected up and down coaxially, the inner diameter of the upper ring part and the lower ring part are the same, and the outer diameter of the upper ring part is smaller than that of the lower ring part The outer diameter of each of the diversion grooves is provided on the upper end face of the upper ring portion, a gap is provided between the outer peripheral wall of the upper ring portion and the intermediate connecting piece, and the outer periphery of the lower ring portion is connected to the The inner wall of the intermediate connecting piece is threadedly connected, and the intermediate pipe interface communicates with the gap.

The above technical scheme utilizes the flushing of the jet water flow to remove the fat particles adhering to the sieve, thereby avoiding the blockage of the sieve holes; using the function of the jet water flow, the fat particles and the liquid in the chamber are continuously mixed and moved, which plays an important role in preventing the fat. The "stirring" effect of cells without damage loosens the adhesion between large and small fat particles, which is conducive to the separation and filtration of fat particles.

Specifically, the apertures of the screen can be set to different specifications.

Specifically, the locating pin mechanism is a locating pin, the locating pin penetrates through the bracket and is inserted into it, and the intermediate connecting piece is provided with a pair of limit insertion holes which are respectively matched with the locating pins, The upper tank body, the intermediate connecting piece and the lower tank body can be rotated horizontally together until the positioning pin is inserted into any one of the limiting holes for locking. A latch is arranged between the tank body and the bracket. When the latch is in a locked state, the position of the tank body can be fixed and its overturning can be restricted. The bracket is made of metal, including a base and two pillars, and the top of the pillars has a groove corresponding to the rotation axis of the tank, which can not only rotate the tank, but also separate the two.

Specifically, the upper tank is in the shape of an inverted funnel; the lower tank is in the shape of a funnel.

The above technical scheme utilizes the unique funnel-shaped shape of the tank, so that the filtered granular fat automatically floats up under the action of buoyancy and is concentrated at the top opening of the funnel-shaped chamber, and is drawn out through a pipeline, so as to realize the automatic collection of the granular fat after sorting. ; Use gentle shaking to generate relative movement between fat particles, thereby reducing the adhesion between fat particles and the screen, and providing floating space for smaller fat particles.

Specifically, the upper tank body is screwed or snap-connected with the intermediate connecting piece; the lower tank body is screwed or snap-connected with the intermediate connecting piece.

Specifically, the lower part of the upper tank body and the upper part of the intermediate connecting piece are sealed and connected by a sealing ring.

Specifically, the lower tank body is sealedly connected between the upper part and the lower part of the intermediate connecting piece through a sealing ring.

Specifically, the upper pipeline interface is provided with an upper valve; the upper valve is connected with a collection bottle.

Specifically, the lower pipeline interface is provided with a lower valve.

Specifically, the middle pipe interface is provided with a middle valve; the middle valve is connected with a flushing liquid infusion bottle.

Specifically, the upper inner periphery of the intermediate connecting piece is provided with a protruding portion, and the screen is provided between the protruding portion and the jet ring.

Specifically, the intermediate connecting piece is provided with a rotating shaft, the rotating shaft is fixedly connected with a rocker arm, and the rocker arm is connected by a connecting rod to a motor that drives it to rotate horizontally around the axis of the rotating shaft. The rotating shaft set in the middle allows the whole device to swing and flip around the rotating shaft.

The present invention also provides a method for screening and purifying granular fat, using the device for screening and purifying granular fat provided by the present invention for screening, comprising the following steps:

1) After coarse filtration, obtain fresh adipose tissue particles from which large fibers are filtered;

2) Install the screen mesh with the first aperture on the described particle fat screening and purification device, then open the lower valve and the upper valve, and inject the fresh adipose tissue obtained in step 1) from the lower pipeline interface particles, then close the lower valve, open the middle valve to inject flushing liquid until the upper pipeline interface has liquid flowing out, and then close the middle valve;

3) Fixing the particle fat screening and purification device on a shaking device to shake, and after starting to shake, open the middle valve continuously or intermittently to rinse with a rinsing liquid;

4) Shake to the upper pipeline interface until no fat flows out, close the shaking device, and collect the granular fat and grease after the primary sieving in the collection bottle, and then the granular fat after the primary sieving is collected. Suck it out, let it stand for a sufficient time, remove the lower layer of water and the upper layer of grease, and use it later;

5) Close all valves, turn the granular fat screening and purification device upside down, that is, rotate 180° horizontally, lock the locking device, open the upper valve, and then open the middle valve to inject the flushing liquid, from the upper one. The pipeline interface obtains primary purified granular fat, and the particle size of the primary purified granular fat is larger than the first pore size;

6) Clean the inside of the granular fat screening and purification device, and repeat steps 2), 3), 4), and 5) in turn to obtain secondary purified granular fat, wherein, in the process of repeating step 2): the The particulate fat screening and purification device is replaced and installed with a screen with a second aperture, and then the first-pass sieved particulate fat obtained in step 4) is injected from the lower pipe interface, and the second aperture is smaller than the first aperture. The pore size of the obtained secondary purified granular fat is between the first pore size and the second pore size.

In the above technical scheme: coarse filtration can remove coarse fibers; in the initial filtration of steps 2), 3), 4), and 5), a screen (pore size 1) is installed according to the set aperture, and the granular fat after the coarse filtration is processed. The tissue is injected into the lower chamber of the tank body from the lower interface, the two chambers of the tank body are filled with flushing fluid, the whole device is placed on the shaking device, and the flushing fluid is injected through the middle pipe interface. The fat particles and grease smaller than the pore size of the screen mesh automatically float up into the collector under the action of buoyancy through the screen mesh; in the secondary filtration of step 6), the screening and purification device is emptied, and the set screen mesh (pore size 2) is replaced. It is smaller than the primary filter screen, and the fat particles that have been sieved once are injected into the lower chamber of the tank body from the lower interface, the two chambers of the tank body are filled with flushing liquid, the whole device is placed on the shaker, and the flushing fluid is injected through the middle interface. , Under the auxiliary action of the shaking table and the built-in jet device, the fat particles and grease smaller than the aperture of the screen will automatically float up through the screen under the action of buoyancy and move out of the screening device; Granular fat in the diameter range.

Further, it also includes step 7): repeating step 6) several times, replacing and installing screens with different apertures each time, and passing the sieved granular fat obtained by the previous repetition into the screening and purification of the granular fat. This purification is carried out in the device, wherein the apertures of the screens installed in each purification are successively reduced; the particle size of the purified granular fat obtained by repeating step 6) is between the sieves used in the adjacent two repetitions. between the apertures.

In the above technical solution, after repeated repetitions, the granular fat within the specified particle size range can be obtained step by step and accurately.

Specifically, the shaking device is a shaking table, and the shaking device is horizontal shaking. Amplitude and frequency can be adjusted according to needs, for example, the frequency is 60-120 times/min.

Specifically, the flushing solution is PBS buffer, balanced solution or physiological saline.

Specifically, in step 3), the middle valve is continuously or intermittently opened for flushing with a flushing solution, for example, the central valve is opened every 1 minute for flushing with a buffer solution.

The shaking time from step 3) to step 4) can be set as required, for example, it can be 10-20 minutes.

On the basis of the above technical solutions, the present invention can also be improved as follows.

Further, the upper and lower tanks can be made of metal and alloy materials, organic chemical materials, glass, etc., and can be directly processed by casting, machining, 3D printing, etc., or pouring, pouring, extrusion molding, firing Manufactured by other processes.

The further beneficial effect of using the above is that devices with different materials and different processes can be applied according to different usage requirements and different costs, such as a one-time-use screening and purification device.

Further, the coarse filtering device and method are as follows: using an object with pores or multiple protrusions on the surface, placing it in the fat suspension for stirring.

A further benefit of using the above is that larger fibrous tissue can be adhered and separated from granular fat.

Further, the coarse filtering device and method are: making the granular fat pass through a grid or mesh with a large gap.

A further beneficial effect of using the above is that the larger fibrous tissue is kept out of the grid or mesh, thereby separating it from the granular fat.

Further, the valve device is a mechanical valve, an electronic valve, and a hydraulic valve.

The further beneficial effects of adopting the above are: improving the use efficiency of the valve and reducing the pollution risk in the operation process.

Further, the directions of the several holes of the jet ring are at a certain angle with the normal direction and face the screen.

The further beneficial effects of the above are: by controlling the diameter, direction and flow rate of the holes, the flushing effect of the water flow on the screen mesh and the intensity of the stirring effect on the granular fat suspension can be precisely controlled.

Further, the material of the sealing ring can be an elastomer of chemical material, and its position can be located on the side and end surfaces where the upper and lower parts of the tank contact the middle, and the cross-sectional shape can be any geometric shape.

The further beneficial effect of adopting the above is that the sealing effect of the tank body is increased.

Further, the screen can be made by one-time molding, 3D printing, laser drilling, mechanical stamping, welding and other processes.

The further beneficial effects of adopting the above are: improving the precision and mechanical strength of the screen.

Further, the shaking device and method are: using any mechanical equipment that can generate shaking, including a shaking table.

The above-mentioned further beneficial effects are: relative motion between fat particles is generated, thereby reducing the adhesion between the fat particles and the screen, and providing a floating space for the smaller fat particles to avoid clogging of the mesh.

Further, the rocking device and method are as follows: the rocker arm is directly connected to the rotating shaft of the tank body, the crankshaft is connected through the connecting rod, and the electric device is connected to the reduction device to drive the tank body to swing.

The further beneficial effect of adopting the above is that the device is integrated and the dependence on other equipment is reduced.

The beneficial effects of the particle fat screening and purification device and method of the present invention are:

The outstanding advantages of the particle fat screening and purification device and method of the present invention are non-destructive and high-efficiency, which are embodied in:

1. Greatly protect the structure of fat particles and reduce the damage to fat cells;

2. The obtained fat particles are of uniform size, precise and controllable;

3. Improve the filtration efficiency and reduce the waste and loss of granular fat;

4. The whole process of closed aseptic operation reduces the risk of contamination;

5. This process can be repeated many times to obtain fat particles of various sizes.

Description of drawings

FIG. 1 is a schematic structural diagram of the front view of the particle fat screening and purification device provided by the present invention.

FIG. 2 is a schematic top-view structural diagram of the particle fat screening and purification device provided by the present invention.

Figure 3 is a schematic side view of the structure of the device for screening and purifying particulate fat provided by the present invention.

4 is a front view of the jet ring in the particle fat screening and purification device provided by the present invention.

FIG. 5 is a top view of the jet ring in the particle fat screening and purification device provided by the present invention.

FIG. 6 is a schematic diagram of the external structure of the particle fat screening and purification device provided by the present invention.

FIG. 7 is an effect diagram of the method for screening and purifying granular fat provided by the present invention.

In the accompanying drawings 1, 2, 3, 4, 5, 6, the structure list represented by each label is as follows:

1. Upper tank, 2. Intermediate connector, 3. Lower tank, 4. Jet ring, 401, diversion groove, 402, upper ring, 403, lower ring, 5, screen, 6, bracket , 7, locating pin, 8, shaft.

Detailed ways

The principles and features of the present invention are described below, and the examples are only used to explain the present invention, but not to limit the scope of the present invention.

In a specific embodiment, as shown in FIGS. 1 , 2 and 3 , the particle fat screening and purification device includes an upper tank body 1 , an annular intermediate connector 2 and a lower tank body 3 which are connected up and down in sequence. The upper part of the upper tank body 1 is provided with an upper pipeline interface, the lower part of the lower tank body 3 is provided with a lower pipeline interface, and the upper tank body 1 is detachably connected to the upper part of the intermediate connector 2 at the lower part, and the lower part of the upper tank body 1 and the upper part of the intermediate connector 2 are detachably connected. The upper part of the intermediate connecting piece 2 is sealedly connected, the lower tank body 3 is detachably connected with the lower part of the intermediate connecting piece 2 at the upper part, and the lower tank body 3 is sealedly connected with the lower part of the intermediate connecting piece 2 at the upper part. A jet ring 4 is detachably connected to the inner ring of the intermediate connecting piece 2 , and a screen 5 is arranged above the jet ring 4 , and the aperture of the screen 5 can be selected from different sizes. The screen 5 is detachably connected to the intermediate connecting piece 2 , and a middle pipe interface leading to the jet flow ring 4 is also provided on the outer surface of the intermediate connecting piece 2 . The particle fat screening and purification device further includes a bracket 6, which is connected with the intermediate connecting piece 2 for horizontal rotation. The bracket 6 is provided with a locking mechanism that restricts the rotation of the intermediate connecting piece 2 around its horizontal axis every half-turn. Based on the particle fat screening and purification device provided by this technical solution, the physical principle that the screen only allows objects smaller than the pore size to pass through can be used to filter and separate fat particles of different sizes; The particles are filtered separately, first use the larger aperture screen, and then use the smaller aperture screen, so as to obtain fat particles with any size between the two apertures, and automatically filter out the grease smaller than the two apertures; using adipose tissue and The specific gravity of oil is lower than that of water, and the principle of buoyancy is applied to make the fat particles automatically float in the liquid and pass through the screen, and concentrate on the top layer of the liquid.

In one embodiment, as shown in FIGS. 4 and 5 , the jet ring 4 includes an annular body, and the body is provided with a plurality of guide grooves 401 on the upper end face of the body in the circumferential direction that communicate with the inner wall and the outer wall; the guide grooves 401 are The straight-line structure has a normal angle of 45° to the outer wall of the body. The body includes a coaxial upper ring portion 402 and a lower ring portion 403. The inner diameters of the upper ring portion 402 and the lower ring portion 403 are the same, the outer diameter of the upper ring portion 402 is smaller than the outer diameter of the lower ring portion 403, and each guide groove 401 is provided with On the upper end face of the upper ring part 402, a gap is provided between the peripheral wall of the upper ring part 402 and the intermediate connecting piece 2, the lower ring part 403 is screwed with the inner wall of the intermediate connecting piece 2 at the outer circumference, and the middle pipe interface communicates with the gap. Based on this technical solution, the fat particles adhering to the sieve can be detached by the flushing of the jet water flow, so as to avoid the blockage of the sieve holes; the function of the jet water flow can make the fat particles and the liquid in the chamber continuously mix and move, which plays a role in The "stirring" effect without damage to fat cells loosens the adhesion between large and small fat particles, which is conducive to the separation and filtration of fat particles.

In one embodiment, the locking mechanism is a positioning pin 7, the positioning pin 7 penetrates through the bracket 6 and is inserted into it, and the middle connecting piece 2 is provided with a pair of limit sockets respectively matching with the positioning pin 7. The body 1 , the intermediate connector 2 and the lower tank body 3 can be rotated horizontally together until the positioning pin 7 is inserted into any one of the limiting holes for locking. Based on this technical solution, the device can be turned over and positioned.

In one embodiment, the upper tank body 1 is in the shape of an inverted funnel; the lower tank body 3 is in the shape of a funnel. This technical solution utilizes the unique funnel-shaped shape of the tank, so that the filtered granular fat will automatically float up under the action of buoyancy and be concentrated at the top opening of the funnel-shaped chamber, and be drawn out through the pipeline, so as to realize the automatic collection of the granular fat after sorting. ; Use gentle shaking to generate relative movement between fat particles, thereby reducing the adhesion between fat particles and the screen, and providing floating space for smaller fat particles.

In one embodiment, the upper pipeline interface is provided with an upper valve; the upper valve communicates with a collection bottle. The lower pipeline interface is provided with a lower valve. The middle pipe interface is provided with a middle valve; the middle valve is connected with a flushing liquid infusion bottle. The intermediate connecting piece 2 is provided with a rotating shaft 8, and the rotating shaft 8 is fixedly connected with a rocker arm and is connected with a motor that drives it to rotate horizontally around the axis of the rotating shaft. Based on this technical solution, the control of the operation process of the equipment can be conveniently realized.

In one embodiment, the upper inner periphery of the intermediate connecting piece 2 is provided with a protruding portion, and the screen 5 is provided between the protruding portion and the jet ring 4 . Based on this technical solution, the installation and replacement of the screen 5 can be conveniently realized.

In one embodiment, the upper tank body 1 is threadedly connected with the intermediate connecting piece 2 ; the lower tank body 3 is threadedly connected with the intermediate connecting piece 2 . Based on this technical solution, the disassembly and assembly of the tank body and the intermediate connecting piece can be realized in an aspect.

In one embodiment, the particle fat screening and purification method specifically includes the following steps:

1) Take 50ml of fresh granular adipose tissue, let it stand for 15 minutes, gently stir several times with two or more porous liposuction needles, discard the fibrous strands adhering to it, add rinsing solution to wash 3 times, and obtain Granular adipose tissue after removal of crude fibers;

2) Install a sieve with an aperture of 3 mm on the particle fat screening and purification device, and tighten the tank body. Connect the upper port of the tank to the collection bottle with an infusion tube, and the middle port of the tank with an infusion tube to connect a flushing liquid infusion bottle. Then inject fresh adipose tissue particles from the lower pipeline interface, then close the lower valve, open the middle valve to inject flushing fluid until the upper pipeline interface flows out, and then close the middle valve;

3) Fix the particle fat screening and purification device on the shaker, turn on the shaker, run at a speed of 100 times/min, and open the valve of the middle interface every 1 minute to rinse for 10 seconds, and continue for 10 minutes until no fat flows out of the infusion tube of the upper interface. ;

4) Shake to the upper pipeline interface until no fat flows out, close the shaking device, and collect the granular fat after sieving once in the collection bottle, then suck out the granular fat after sieving once, and let it stand for 15 minutes to remove the lower layer of water and moisture. After the upper layer of grease is used;

5) Turn the granular fat screening and purification device upside down and fix it, then clean the device with a flushing liquid from the central valve, and collect the primary purified granular fat at the pipe interface located above, and its particle size is greater than 3 mm;

6) Clean the inside of the granular fat screening and purification device, and repeat steps 2), 3), 4), and 5) in turn to obtain secondary purified granular fat. The diameter of the secondary purified granular fat is greater than 2 mm and less than 3 mm. Wherein, in the process of repeating step 2): replace the particle fat screening and purification device with a 2mm sieve with an aperture, and then inject the sieved granular fat from the interface of the lower pipeline.

7) Repeat steps 5) and 6) several times, and replace and install screens with different apertures in each repetition. The fat is passed into the granular fat screening and purification device to carry out this purification, wherein the pore size of the screen installed in each time is successively reduced. Finally, granular fats of different sizes with diameters of 1-2 mm, 0.5-1 mm, 0.2-0.5 mm, 0.1-0.2 mm were obtained.

The effect of the purified granular fat obtained in each step is shown in Figure 3. In each test tube, the part with a white color is the purified granular fat obtained.

The above are only preferred embodiments of the present invention and are not intended to limit the present invention. Any modifications, equivalent replacements, improvements, etc. made within the spirit and principles of the present invention shall be included in the protection of the present invention. within the range.

Claims (9)

1. a particle fat screening and purification device, is characterized in that, comprises upper tank body (1), annular intermediate connector (2) and lower tank body (3) that are connected up and down successively, and the upper tank body (3). The upper part of 1) is provided with an upper pipeline interface, the lower part of the lower tank body (3) is provided with a lower pipeline interface, and the lower part of the upper tank body (1) is detachable from the upper part of the intermediate connecting piece (2). Sealing connection, the upper part of the lower tank body (3) is detachably sealed connection with the lower part of the intermediate connecting piece (2), and a jet ring (4) is detachably connected to the inner ring of the intermediate connecting piece (2). ), a screen (5) is provided above the jet ring (4), the screen (5) is detachably connected to the intermediate connecting piece (2), and the intermediate connecting piece (2) The outer surface of the device is also provided with a middle pipe interface leading to the jet ring (4). For the horizontal rotation connection, the bracket (6) is provided with a locking mechanism that restricts the rotation of the intermediate connecting piece (2) around its horizontal axis every half-turn. 2 . The particle fat screening and purification device according to claim 1 , wherein the jet ring ( 4 ) comprises a ring-shaped body, and the body is provided with a plurality of inner and outer walls in a circumferential direction on the upper end face. 3 . The guide groove (401); the guide groove (401) is a linear structure, and the normal angle between it and the outer wall of the body is 0-45°. 3. The particle fat screening and purification device according to claim 2, characterized in that: the body comprises an upper ring portion (402) and a lower ring portion (403) that are fixedly connected up and down coaxially, and the upper ring portion (402) ) and the inner diameter of the lower ring part (403) are the same, the outer diameter of the upper ring part (402) is smaller than the outer diameter of the lower ring part (403), and each of the guide grooves (401) is provided in the On the upper end surface of the upper ring portion (402), a gap is provided between the outer peripheral wall of the upper ring portion (402) and the intermediate connecting piece (2), and the outer periphery of the lower ring portion (403) is connected to the The inner wall of the middle connecting piece (2) is screwed, and the middle pipe interface communicates with the gap. 4. The particle fat screening and purification device according to claim 1, characterized in that: the locking mechanism is a positioning pin (7), and the positioning pin (7) penetrates the bracket (6) and is inserted into it, The middle connecting piece (2) is provided with a pair of limit insertion holes which are respectively matched with the positioning pins (7), and the upper tank body (1), the middle connecting piece (2) and the The lower tank body (3) can be rotated horizontally together until the positioning pin (7) is inserted into any one of the limiting holes for locking. 5. The particle fat screening and purification device according to any one of claims 1 to 4, characterized in that: Described upper tank body (1) is inverted funnel shape; The lower tank body (3) is funnel-shaped; The upper tank body (1) is screwed or snap-connected with the intermediate connecting piece (2); the lower tank body (3) is screwed or snap-connected with the intermediate connecting piece (2); The lower part of the upper tank body (1) and the upper part of the intermediate connecting piece (2) are sealed and connected by a sealing ring (8); The upper part of the lower tank body (3) and the lower part of the intermediate connecting piece (2) are sealedly connected by a sealing ring (8); The upper pipeline interface is provided with an upper valve; the upper valve is communicated with a collection bottle; The lower pipeline interface is provided with a lower valve; The middle pipe interface is provided with a middle valve; the middle valve is connected with a flushing liquid infusion bottle; The upper inner periphery of the intermediate connecting piece (2) is provided with a protruding portion, and the screen (5) is arranged between the protruding portion and the jet ring (4); The apertures of the screen (5) can be set to different specifications. 6 . The particle fat screening and purification device according to claim 5 , wherein the intermediate connecting piece ( 2 ) is provided with a rotating shaft, the rotating shaft is fixedly connected with a rocker arm, and the rocker arm is connected by a connecting rod to drive it. 7 . A motor that rotates horizontally about the axis of the shaft. 7. A method for screening and purifying particulate fat, using the device for screening and purifying particulate fat according to claim 5 or 6 for screening, wherein the method comprises the following steps: 1) After coarse filtration, obtain fresh adipose tissue particles from which large fibers are filtered; 2) Install the screen mesh (5) with the first aperture on the particle fat screening and purification device, open the upper valve and the lower valve, and inject the fresh water obtained in step 1) from the lower pipeline interface. Adipose tissue particles, close the lower valve, then open the middle valve to inject flushing fluid until the upper pipeline interface flows out, and then close the middle valve; 3) Fixing the particle fat screening and purification device on a shaking device to shake, and after starting to shake, open the middle valve continuously or intermittently to rinse with a rinsing liquid; 4) Shake to the upper pipeline interface until no fat flows out, close the shaking device, and collect the granular fat and grease after sieving once in the collection bottle, and then suck out the granular fat after sieving once, Let it stand, remove the lower layer of moisture and upper layer of grease, and then use it; 5) Close all valves, turn the particle fat screening and purification device upside down, lock the locking device, open the upper valve, then open the middle valve to inject the flushing liquid, and obtain a purified one from the upper pipeline interface. Granular fat, the particle size of the primary purified granular fat is larger than the first pore size; 6) Clean the inside of the granular fat screening and purification device, and repeat steps 2), 3), 4), and 5) in turn to obtain secondary purified granular fat, wherein, in the process of repeating step 2), the The particle fat screening and purification device is replaced and installed with a screen mesh (5) with a second aperture, and then the once-screened particle fat obtained in step 4) is injected from the lower pipe interface, and the second aperture is smaller than the second aperture. the first pore size; the particle size of the secondary purified granular fat is between the first pore size and the second pore size. 8. The method for screening and purifying granular fat according to claim 7, further comprising step 7): repeating step 6) several times, replacing and installing screens (5) with different apertures each time, and the above The sieved granular fat obtained by one repetition is passed into the granular fat screening and purification device to carry out this purification, wherein the pore size of the screen (5) installed in each purification is successively reduced; the steps are repeated each time. 6) The particle size of the obtained purified granular fat is between the apertures of the sieves (5) used for two adjacent repetitions respectively. 9. The method for screening and purifying particulate fat according to any one of claims 7 to 8, characterized in that: The shaking device is a shaking table; the method of the shaking device is horizontal shaking; The flushing solution is PBS buffer, balance solution or physiological saline.

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