CN111686167A

CN111686167A - Preparation method and application of antibacterial and anti-inflammatory ointment

Info

Publication number: CN111686167A
Application number: CN202010684409.5A
Authority: CN
Inventors: 张晓君
Original assignee: Chengdu Yangtian Wanying Pharmaceutical Co ltd
Current assignee: Chengdu Yangtian Wanying Pharmaceutical Co ltd
Priority date: 2020-07-16
Filing date: 2020-07-16
Publication date: 2020-09-22

Abstract

The invention relates to the technical field of skin disinfection preparations, and provides a preparation method of a bacteriostatic anti-inflammatory ointment, which at least comprises the following steps: (1) mixing and stirring the humectant and the thickener, adding the mixture into the solvent, stirring and heating the mixture to 60-90 ℃, adding the preservative, the first skin conditioner, the first bactericide and the second skin conditioner, and mixing and stirring the mixture to obtain a phase A; (2) separately mixing emulsifier, emollient, and antioxidant, stirring, heating to 60-90 deg.C, and adding to obtain phase B; (3) keeping the temperature at 60-90 ℃, mixing the phase B and the phase A, homogenizing for 1-60 minutes, and cooling to obtain a premix; (4) adding the second bactericide and the aromatic into the premix, mixing and filtering to obtain the bactericide. Can be used for treating skin problems such as allergic skin, erythra skin, acne skin, scalp itch, etc., and has the effects of promoting wound healing, killing bacteria, and relieving inflammation.

Description

Preparation method and application of antibacterial and anti-inflammatory ointment

Technical Field

The invention relates to the technical field of skin disinfection preparations, in particular to a preparation method and application of an antibacterial and anti-inflammatory ointment.

Background

The rash is wheal and hot flush, has different sizes and shapes, often occurs suddenly and appears in batches, can be rapidly subsided after hours, does not leave traces after subsidence, but often attacks repeatedly, has subjective itching, can be accompanied by abdominal pain, nausea, vomiting, chest distress, palpitation and dyspnea, and has few symptoms of fever, arthrocele, hypotension, shock, edema of larynx and suffocation; acne is a chronic inflammatory skin disease of a pilosebaceous unit, mainly occurs to teenagers, has great influence on the psychology and the social interaction of the teenagers, and can be naturally relieved or healed after adolescence. The clinical manifestation is characterized by polymorphic skin lesions such as pimples, pustules, nodules and the like which are easy to be found on the face, a plurality of microorganisms in hair follicles, particularly propionibacterium acnes, are propagated in a large quantity, lipase produced by the propionibacterium acnes decomposes sebum to generate free fatty acid, and simultaneously chemotaxis inflammatory cells and mediators, and finally, the inflammatory reaction is induced and aggravated.

The ointment is a uniform semisolid external preparation prepared by mixing the medicine with an oleaginous or water-soluble matrix. The ointment is mainly used for skin and mucous membrane surface in medical treatment, and has local protection and treatment effects. The ointment can be tightly adhered and adhered for a long time, is spread on the surface of the skin, and has the functions of local treatment and systemic treatment. However, the soft plaster for diminishing inflammation and relieving itching prepared by the technical method adopted by the prior art has the disadvantages that the dosage of the effective components and the stability of the ointment are in great contradiction due to simple preparation process, the skin is stimulated by too many effective medicines, the stability is poor, the effect cannot be well played if too few effective medicines are used, and the use effect is poor.

Disclosure of Invention

In order to solve the above technical problems, a first aspect of the present invention provides a method for preparing a bacteriostatic anti-inflammatory ointment, comprising at least the steps of: (1) mixing and stirring the humectant and the thickener, adding the mixture into the solvent, stirring and heating the mixture to 60-90 ℃, adding the preservative, the first skin conditioner, the first bactericide and the second skin conditioner, and mixing and stirring the mixture to obtain a phase A; (2) separately mixing emulsifier, emollient, and antioxidant, stirring, heating to 60-90 deg.C, and adding to obtain phase B; (3) keeping the temperature at 60-90 ℃, mixing the phase B and the phase A, homogenizing for 1-60 minutes, and cooling to obtain a premix; (4) adding the second bactericide and the aromatic into the premix, mixing and filtering to obtain the bactericide.

As a preferable technical scheme, in the step (3) of the invention, the vacuum degree in the homogenizing process is-0.03-0.01 Mpa.

In a preferred embodiment, the first skin conditioning agent is at least one selected from the group consisting of allantoin, caprylic acid glyceride, hexyldecanol, bisabolol, cetyl N-palmitoyl hydroxyproline, and stearic acid; the second skin conditioner is selected from at least one or more of radix Angelicae sinensis, rhizoma Ligustici Chuanxiong, Saviae Miltiorrhizae radix, rhizoma picrorhizae, Borneolum Syntheticum, Catechu, berberine hydrochloride, Chinese ink, artificial Moschus, artificial calculus bovis, fel Ursi powder, and bilis Bovina.

In a preferred embodiment, the first skin conditioning agent is allantoin; the second skin conditioner is selected from at least one or more of rhizoma picrorhizae, Borneolum Syntheticum, Catechu, berberine hydrochloride, XIANGMO, artificial Moschus, artificial calculus bovis, fel Ursi powder, and bilis Bovina; the weight ratio of the first skin conditioning agent to the second skin conditioning agent is (0.01-0.1): 1.

in a preferred embodiment, the emulsifier in the present invention is selected from one or more of fatty acid ester series, polyglycerol-3 methyl glucose distearate, cetostearyl alcohol, glycerol, polyoxyl stearate, polyoxyethylene lauryl ether, alkyl glycoside, and caprylic/capric triglyceride.

In a preferred embodiment, the fatty acid ester series in the present invention includes at least one of polyethylene glycol stearate, glycerin fatty acid ester, and propylene glycol fatty acid ester; the saponification value of the polyethylene glycol stearate is 85-120 mgKOH/g; the saponification value of the glycerol fatty acid ester is 160-220 mgKOH/g.

As a preferred embodiment, the emollient is selected from one or more of cyclosiloxane complex, isooctyl palmitate, ethylhexyl palmitate, petrolatum, beeswax, caprylic/capric triglyceride.

As a preferable technical scheme, the frequency of the step (1) in the invention during mixing and stirring is 15-45 Hz; the frequency of the homogenization in the step (3) is 30-120 Hz.

As a preferable technical scheme, the pore diameter of the filtration in the step (4) in the invention is 50-200 meshes.

The second aspect of the invention provides an application of the antibacterial and anti-inflammatory ointment, wherein the antibacterial and anti-inflammatory ointment is prepared by the preparation method of the antibacterial and anti-inflammatory ointment; the antibacterial and anti-inflammatory ointment is applied to at least one of allergic skin, erythra skin, acne skin and scalp itch.

Compared with the prior art, the invention has the following excellent beneficial effects:

the antibacterial and anti-inflammatory ointment prepared by the preparation method can be applied to treating skin problems such as allergic skin, erythra skin, acne skin, scalp itch and the like, is quick in effect taking speed, and has the effects of promoting wound healing, sterilizing and diminishing inflammation on wounds. In addition, although the content of the effective components adopted in the disinfectant ointment prepared by the invention is not high, the therapeutic effect after application is very obvious, particularly, the disinfectant ointment has the most obvious effect of relieving itching and sterilizing, and has very excellent temperature stability under high and low temperature environments.

Detailed Description

The technical features of the technical solutions provided by the present invention will be further clearly and completely described below with reference to the specific embodiments, and it should be apparent that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

It will be understood by those skilled in the art that, unless otherwise defined, all terms (including technical and scientific terms) used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. It will be further understood that terms, such as those defined in commonly used dictionaries, should be interpreted as having a meaning that is consistent with their meaning in the context of the prior art and will not be interpreted in an idealized or overly formal sense unless expressly so defined herein.

The words "preferred", "preferably", "more preferred", and the like, in the present invention, refer to embodiments of the invention that may provide certain benefits, under certain circumstances. However, other embodiments may be preferred, under the same or other circumstances. Furthermore, the recitation of one or more preferred embodiments does not imply that other embodiments are not useful, nor is it intended to exclude other embodiments from the scope of the invention.

The invention provides a preparation method of a bacteriostatic and anti-inflammatory ointment, which at least comprises the following steps:

(1) mixing and stirring the humectant and the thickener, adding the mixture into the solvent, stirring and heating the mixture to 60-90 ℃, adding the preservative, the first skin conditioner, the first bactericide and the second skin conditioner, and mixing and stirring the mixture to obtain a phase A;

(2) separately mixing emulsifier, emollient, and antioxidant, stirring, heating to 60-90 deg.C, and adding to obtain phase B;

(3) keeping the temperature at 60-90 ℃, mixing the phase B and the phase A, homogenizing for 1-60 minutes, and cooling to obtain a premix;

(4) adding the second bactericide and the aromatic into the premix, mixing and filtering to obtain the bactericide.

In some preferred embodiments, the method for preparing the antibacterial and anti-inflammatory ointment at least comprises the following steps:

(1) mixing and stirring the humectant and the thickener, adding the mixture into the solvent, stirring and heating the mixture to 80 ℃, adding the preservative, the first skin conditioner, the first bactericide and the second skin conditioner, and mixing and stirring the mixture to obtain a phase A;

(2) separately mixing emulsifier, emollient, and antioxidant, stirring, heating to 80 deg.C, and adding to obtain phase B;

(3) keeping the temperature at 80 ℃, mixing the phase B and the phase A, homogenizing for 5 minutes, and cooling to obtain a premix;

In some embodiments, in the step (3), the vacuum degree in the homogenizing process is-0.03-0.01 Mpa; preferably, in the step (3), the degree of vacuum in the homogenizing process is-0.03 MPa.

The antibacterial and anti-inflammatory ointment prepared by the preparation method of the antibacterial and anti-inflammatory ointment can be applied to treating skin problems such as allergic skin, erythra skin, acne skin, scalp itch and the like, is quick in effect taking speed, and has the effects of promoting wound healing, sterilizing and diminishing inflammation on wounds. In addition, although the content of the effective components adopted in the disinfectant ointment prepared by the invention is not high, the therapeutic effect after application is very obvious, particularly, the disinfectant ointment has the most obvious effect of relieving itching and sterilizing, and has very excellent temperature stability under high and low temperature environments.

The inventor thinks that because the use amount of the coptis chinensis is high, the use cost of the disinfection ointment is increased, the use cost of the disinfection ointment is greatly reduced by replacing the coptis chinensis with the berberine hydrochloride, the berberine hydrochloride has extremely obvious inhibiting effect on dysentery bacillus, escherichia coli, pneumococcus pneumoniae, staphylococcus aureus, streptococcus, typhoid bacillus, amoeba protozoon and the like, but the inventor finds that the effective time of relieving itching and diminishing inflammation is prolonged after the berberine hydrochloride is added, the effect of diminishing inflammation and relieving itching is difficult to be quickly achieved, but the inventor finds that the effective time of the disinfection ointment can be increased and the effect is enhanced after a small amount of allantoin is added, the inventor thinks that the catechu content is more, the catechu tannic acid is more, the interaction is easy to occur between the catechu tannic acid after the berberine hydrochloride is added, and the sterilization effect of the catechu and the, firstly, adding allantoin into high-temperature hot water for mixing, then adding a second skin conditioner consisting of traditional Chinese medicines, mixing the allantoin with components such as cyclosiloxane compound in an emollient, fatty acid ester series in an emulsifier, polyglycerol-3-methylglucdistearate and the like in the same high-temperature hot water, reducing the interaction between berberine hydrochloride and catechu tannic acid, carrying nitrogen-containing effective active groups on the surface of the allantoin, wherein the fatty acid ester series contains more free fatty acids, and promoting polar interaction between nitrogen-containing elements on the surface of the allantoin and functional groups of free fatty acids in the fatty acids under high-temperature environmental conditions, so that the dispersing power of oil-in-water emulsion particles in ointment in water is increased, and the stability of a system is improved.

In some embodiments, the first skin conditioning agent, the second skin conditioning agent, together, are present in an amount of 1-5% by weight of the solvent; preferably, the total amount of the first skin conditioning agent and the second skin conditioning agent is 2.5% by weight of the solvent.

In some embodiments, the first skin conditioning agent is selected from at least one of allantoin, glyceryl caprylate, hexyldecanol, bisabolol, cetyl N-palmitoyl hydroxyproline, stearic acid; the second skin conditioner is selected from at least one or more of radix Angelicae sinensis, rhizoma Ligustici Chuanxiong, Saviae Miltiorrhizae radix, rhizoma picrorhizae, Borneolum Syntheticum, Catechu, berberine hydrochloride, Chinese ink, artificial Moschus, artificial calculus bovis, fel Ursi powder, and bilis Bovina.

In some preferred embodiments, the first skin conditioning agent is allantoin and the second skin conditioning agent is a combination of picrorhiza rhizome, borneol, catechu, berberine hydrochloride, balsam, artificial musk, artificial bezoar, bear gall powder, ox bile; the weight ratio of the first skin conditioning agent to the second skin conditioning agent is (0.01-0.1): 1; more preferably, the weight ratio between the first skin conditioning agent and the second skin conditioning agent is 0.05: 1.

in some embodiments, the weight ratio of picrorhiza rhizome, borneol, catechu, berberine hydrochloride, balsam, artificial musk, artificial bezoar, bear gall powder and oxgall is 1: (0.01-0.1): (0.5-1.5): (0.01-0.1): (1.5-2.5): (0.01-0.1): (0.01-0.1): (0.1-0.5): (1-2); more preferably, the weight ratio of picrorhiza rhizome, borneol, catechu, berberine hydrochloride, fragrant ink, artificial musk, artificial bezoar, bear gall powder and ox bile is 1: 0.06: 1: 0.065: 2: 0.05: 0.05: 0.2: 1.6.

the components of the traditional Chinese medicine composition are introduced in sequence as follows:

rhizoma picrorhizae: the English name is RHIZOMA PICRORHIZAE, pseudorhizoma Coptidis, and is dried rhizome of Picrorhiza scrophulariiflora Pennell of Scrophulariaceae. The rhizoma picrorhizae water diffusion agent (1:4) has different degrees of inhibition effects on dermatophytes such as blue trichophyton seedlings and the like in a test tube. The root extract has choleretic and antibacterial effects, and can be used for treating hepatitis and urinary tract infection.

Borneol: the English name BORNEOLUM SYNTHETIC is also called Borneolum, folium Artemisiae Argyi powder, and Borneolum, which are processed products of Borneolum resin of Dipterocarpaceae, or processed products of Camphora, oleum Terebinthinae, etc. synthesized by chemical method. The functions are mainly to dredge orifices, dispel stagnated fire, remove nebula, improve eyesight, reduce swelling and alleviate pain. It is indicated for wind stroke, vomiting, coma due to febrile disease, epilepsy due to convulsion, phlegm-phlegm, qi block, deafness, sore throat, aphtha, otitis media, carbuncle, swelling, hemorrhoid, nebula, and enterobiasis.

Catechu: the product is dried soft extract of peeled branch and dried stem of Acacia catechu (L.f.) of Albizia of Leguminosae. The Catechu decoction has inhibitory effect on Staphylococcus aureus, Bacillus diphtheriae, Bacillus proteus, Bacillus dysenteriae and Bacillus typhi; it also has inhibitory effect on common pathogenic dermatophytes. The extract of the leaf has inhibitory effect on Staphylococcus aureus and Escherichia coli.

Berberine hydrochloride: it has inhibitory effect on dysentery bacillus, Escherichia coli, Diplococcus pneumoniae, Staphylococcus aureus, Streptococcus, typhoid bacillus and amoeba protozoon. The medicine is mainly used for treating intestinal infection, bacillary dysentery and the like in clinic.

Fragrant ink: the Chinese medicinal herb Xiangmo is a pungent and mild Chinese medicinal material, has the main effects of stopping bleeding and reducing swelling of heart channels, liver channels and kidney channels, can be clinically used for treating various common diseases such as hematemesis, metrorrhagia, metrostaxis, bloody flux and the like of human beings, and has particularly excellent treatment effect. Besides, the fragrant ink can also reduce swelling and relieve pain, and has excellent treatment effect on human back carbuncle and swelling. The Chinese medicinal composition has hemostatic and repercussive effects, and can be used for treating hematemesis, epistaxis, metrorrhagia, dysentery, carbuncle, swelling, and backache.

Artificial musk: the artificial musk is used as a substitute of artificial musk of endangered animal medicinal materials, is identical to a natural artificial musk formula, is a national important scientific research result and a confidential variety, nearly 400 Chinese patent medicine varieties are produced by using the artificial musk, covers common dosage forms of the Chinese patent medicines, is a great breakthrough in the research of rare animal medicinal material substitutes, opens up an important new way for the application of other rare animal medicinal materials, creates a referential mode for the popularization of other scientific research results, and promotes the sustainable and healthy development of the traditional Chinese medicine industry.

Artificial bezoar: artificial bezoar, name of traditional Chinese medicine. The product is prepared from ox gall powder, cholic acid, hyodeoxycholic acid, taurine, bilirubin, cholesterol, trace elements, etc. Is yellow loose powder. Has the effects of clearing away heat and toxic material, eliminating phlegm and arresting convulsion. It is often used for treating phlegm-heat, delirium, coma, acute infantile convulsion, sore throat, aphtha of the mouth and tongue, carbuncle, furuncle, etc.

Bear gall powder: bear gall powder has the functions of clearing heat, calming liver and improving eyesight. It can be used for treating conjunctival congestion, swelling and pain of throat. The main treatment functions of clearing away heat and toxic material, improving eyesight and relieving spasm. Can be used for treating infantile convulsion due to excessive heat, epilepsy, convulsion, and jaundice; it is externally used for abscess, hemorrhoid, conjunctival congestion and nebula.

And (3) ox bile: is prepared from gallbladder or bile of cattle or buffalo, wherein the bile mainly contains sodium cholate, bile pigment, mucin, small amount of fat, soap, cholesterol, lecithin, choline, urea, and inorganic salts such as sodium chloride, calcium phosphate, and iron phosphate.

In the invention, picrorhiza rhizome, borneol, catechu, berberine hydrochloride, fragrant ink, artificial musk, artificial bezoar, bear gall powder and ox bile are mainly adopted as main functional components, and the prepared disinfection ointment for skin mucosa has good curative effects of relieving itching and diminishing inflammation, particularly has the characteristics of quick response, no side effect and no recurrence, and improves the safety and the usability of medication.

In some embodiments, the emulsifier is present in an amount of 10 to 20% by weight of the total solvent; preferably, the emulsifier is used in an amount of 15% by weight based on the total weight of the solvent.

In some embodiments, the emulsifier is selected from the group consisting of a combination of one or more of the fatty acid ester series, polyglycerol-3 methyl glucose distearate, cetearyl alcohol, glycerol, polyoxyl stearate, polyoxyethylene lauryl ether, alkyl glycosides, caprylic/capric triglycerides; preferably, the emulsifier is selected from the group consisting of fatty acid esters, polyglyceryl-3-methylglucdistearate, cetearyl alcohol.

In some embodiments, the weight ratio between the series of fatty esters, polyglycerol-3 methylglucose distearate, cetearyl alcohol is 1: (0.5-1.5): (1.5-2.5); preferably, the weight ratio between the series of fatty esters, polyglycerol-3 methylglucose distearate and cetearyl alcohol is 1: 1: 2.

in some embodiments, the series of fatty acid esters includes at least one of polyethylene glycol stearate, glycerin fatty acid ester, propylene glycol fatty acid ester; the saponification value of the polyethylene glycol stearate is 85-120 mgKOH/g; the saponification value of the glycerol fatty acid ester is 160-220 mgKOH/g.

In some embodiments, the series of fatty acid esters includes a combination of polyethylene glycol stearates, glycerin fatty acid esters; the weight ratio of the polyethylene glycol stearate to the glycerin fatty acid ester is 1: (0.5-1.5); preferably, the weight ratio of the polyethylene glycol stearate to the glycerin fatty acid ester is 1: 1.

in some preferred embodiments, the polyethylene glycol stearate has a saponification value of 102-118 mgKOH/g; the saponification value of the glycerol fatty acid ester is 163-170 mgKOH/g.

In some embodiments, the glycerol fatty acid ester is selected from glycerol monostearate, glycerol monooleate, glycerol monolaurate; preferably, the glycerol fatty acid ester is glycerol monostearate.

In some embodiments, the emollient is present in an amount of 10 to 15% by weight of the total solvent; preferably, the emollient is present in an amount of 12% by weight of the total solvent.

In some embodiments, the emollient is selected from the group consisting of a combination of one or more of cyclosiloxane complex, isooctyl palmitate, ethylhexyl cetyl alcohol, petrolatum, beeswax, caprylic/capric triglyceride; preferably, the emollient is selected from the group consisting of cyclosiloxane complex, isooctyl palmitate, ethylhexyl cetyl alcohol, petrolatum.

In some embodiments, the weight ratio of the cyclosiloxane complex, isooctyl palmitate, ethylhexyl cetyl ester, petrolatum is 1: (1-1.5): (0.5-1.5): (0.5-1); preferably, the weight ratio of the cyclosiloxane complex, the isooctyl palmitate, the ethylhexyl cetyl alcohol and the petrolatum is 1: 1.3: 1: 0.8.

in some embodiments, the cyclosiloxane complex is cyclopentadimethylsiloxane and/or cyclohexasiloxane; preferably, the weight ratio of the cyclopentadimethylsiloxane to the cyclohexasiloxane is 1: (0.01-5); preferably, the weight ratio of the cyclopentadimethylsiloxane to the cyclohexasiloxane is 1: (0.5-3); more preferably, the weight ratio of the cyclopentadimethylsiloxane to the cyclohexasiloxane is 1: 2.

the inventor finds that the phenomena of layering and precipitation can occur in the disinfection ointment prepared by the invention, but the inventor surprisingly finds that the stability of the disinfection ointment can be remarkably improved by compounding the proper cyclosiloxane complex and the emulsifier of the fatty acid ester series in the system, the disinfection ointment can be kept stable in high-temperature and low-temperature environments for a long time, and the skin is also remarkably improved to be fine and smooth, the inventor considers that the synergy effect can occur between the allantoin, the cyclosiloxane complex and the emulsifier of the fatty acid ester series, the surface of the allantoin carries effective active groups containing nitrogen, the fatty acid ester series contains more free fatty acids, the nitrogen-containing elements on the surface of the allantoin and the functional groups of the free fatty acids in the allantoin generate polar interaction, and the dispersion force of oil-in-water emulsion particles in the ointment in water is increased, the stability of the system is improved, after the disinfection ointment is applied to the skin of a human body, nitrogen active groups contained in the surface of allantoin can soften keratin, the permeation of skin mucosa to a medicinal composition is increased, the medicinal effect is improved, a proper amount of cyclosiloxane compound is added, the cohesive energy in the system can be reduced, the application effect of the anti-inflammatory ointment is facilitated, the ointment can be finely and uniformly dispersed on the surface of the skin during application, the active permeation of the skin mucosa to effective components is more rapidly facilitated, a film can be formed on the surface of the skin, the water-locking effect of the skin surface layer can be increased, and the fineness and the smoothness of the skin are improved.

In some embodiments, the humectant is added in an amount of 0.5 to 1% of the solvent; preferably, the addition amount of the humectant is 0.85% of the dosage of the solvent.

In some embodiments, the humectant is selected from the group consisting of one or more of C2-C4 small molecule alcohols, sodium hyaluronate, sorbitol, amino acid humectants, sodium alginate, hyaluronic acid; the C2-C4 small molecular alcohol is at least one selected from 1, 3-propylene glycol, glycerol, butanediol and glycerol.

In some preferred embodiments, the humectant is a small molecule alcohol of C2-C4; the C2-C4 small molecular alcohol is a combination of 1, 3-propylene glycol and glycerol; preferably, the weight ratio of the 1, 3-propylene glycol to the glycerol is 1: (0.5-1); more preferably, the weight ratio of the 1, 3-propylene glycol to the glycerol is 1: 0.75.

in some embodiments, the thickener is used in an amount of 0.1 to 1% by weight based on the total weight of the solvent; preferably, the thickener is used in an amount of 0.2% by weight based on the total weight of the solvent.

In some embodiments, the thickening agent is selected from at least one of hydroxyethylcellulose, carbomer, sodium alginate; preferably, the thickener is hydroxyethyl cellulose.

In some embodiments, the preservative is present in an amount of 0.1 to 1% by weight of the total solvent; preferably, the thickener is used in an amount of 0.2% by weight based on the total weight of the solvent.

The type of the preservative in the invention is not particularly limited, and preferably, the preservative is chlorphenesin.

In some embodiments, the solvent is at least one of water, ethanol, glycerol; preferably, the solvent is water.

In some embodiments, the total amount of the first bactericide and the second bactericide in the present invention is 1-5% of the total weight of the solvent; preferably, the total amount of the first bactericide and the second bactericide is 2.5% of the total weight of the solvent.

The types of the first bactericide and the second bactericide are not limited respectively, and the bactericides used in the general field can be used; preferably, the first bactericide is cetylpyridinium chloride; the second bactericide is polyaminopropyl biguanide.

In some embodiments, the frequency of the mixing agitation in step (1) is 15 to 45 Hz; the frequency of the homogenization in the step (3) is 30-120 Hz.

In some preferred embodiments, the frequency of the mixing and stirring in step (1) is 30 Hz; and (3) the frequency during homogenizing in the step (3) is 60 Hz.

In some embodiments, the pore size upon filtration in step (4) is 50-200 mesh; preferably, the pore size in the filtration in step (4) is 100 mesh.

In addition, the proper amount of fragrant ink is added in the invention, the recovery of the antibacterial and anti-inflammatory ointment on the skin of a focus can be obviously improved, the swelling and pain can be relieved, and the antibacterial and anti-inflammatory ointment can generate excellent synergistic effect with other components of the traditional Chinese medicine composition in a compounding way, but if the fragrant ink is used, the color of the ointment becomes dark, and the use experience of a user is influenced. However, the inventor unexpectedly finds that when the allantoin and cyclosiloxane compound, the fatty acid ester series emulsifier and the traditional Chinese medicine composition added with the fragrant ink are compounded for use, the hemostatic and bactericidal effects of the ointment can be improved, the delicate feeling of the disinfection ointment can be improved, the ointment can be applied to a thin layer when in use, the effect can be rapidly exerted, a user can use the ointment for a short time, and the unattractive user experience brought by the fragrant ink is avoided.

The second aspect of the invention provides application of the antibacterial and anti-inflammatory ointment, wherein the antibacterial and anti-inflammatory ointment is prepared by the preparation method of the antibacterial and anti-inflammatory ointment; the antibacterial and anti-inflammatory ointment is applied to at least one of allergic skin, erythra skin, acne skin and scalp itch.

The present invention will be specifically described below by way of examples. It should be noted that the following examples are only for illustrating the present invention and should not be construed as limiting the scope of the present invention, and that the insubstantial modifications and adaptations of the present invention by those skilled in the art based on the above disclosure are still within the scope of the present invention.

Example 1

A preparation method of antibacterial and anti-inflammatory ointment at least comprises the following steps:

In the step (3), the vacuum degree in the homogenizing process is-0.03 Mpa.

The total amount of the first skin conditioning agent and the second skin conditioning agent is 2.5% by weight of the solvent.

The first skin conditioner is allantoin, and the second skin conditioner is the combination of picrorhiza rhizome, borneol, catechu, berberine hydrochloride, fragrant ink, artificial musk, artificial bezoar, bear gall powder and ox bile; the weight ratio of the first skin conditioning agent to the second skin conditioning agent is 0.05: 1. the weight ratio of rhizoma picrorhizae, borneol, catechu, berberine hydrochloride, fragrant ink, artificial musk, artificial bezoar, bear gall powder and ox bile is 1: 0.06: 1: 0.065: 2: 0.05: 0.05: 0.2: 1.6.

the dosage of the emulsifier is 15 percent of the total weight of the solvent. The emulsifier is selected from the group consisting of fatty acid esters, polyglyceryl-3-methylglucidistearate, cetearyl alcohol. The weight ratio of the fatty acid ester series, the polyglycerol-3 methyl glucose distearate and the cetearyl alcohol is 1: 1: 2. polyglyceryl-3 methylglucose distearate brand as TEGOCARE 450; the fatty acid ester series comprise polyethylene glycol stearate and glycerol monostearate, wherein the weight ratio of the polyethylene glycol stearate to the glycerol fatty acid ester is 1: 1. the polyethylene glycol stearate is PEG-100 stearate with model of SG-6, and is purchased from Ghan Hua Zhixiang scientific biotechnology, Inc.; the glycerol monostearate was purchased from south china jiuze chemical company under the code GMS.

The amount of the emollient is 12% of the total weight of the solvent. The emollient is selected from the group consisting of cyclosiloxane complex, isooctyl palmitate, ethylhexyl cetyl, petrolatum. The weight ratio of the cyclosiloxane compound to the isooctyl palmitate to the ethylhexyl cetyl alcohol to the petrolatum is 1: 1.3: 1: 0.8. the cyclosiloxane compound is cyclopentadimethylsiloxane and cyclohexasiloxane; the weight ratio of the cyclopentadimethylsiloxane to the cyclohexasiloxane is 1: 2.

the addition amount of the humectant is 0.85 percent of the dosage of the solvent. The humectant is a combination of 1, 3-propylene glycol and glycerol; the weight ratio of the 1, 3-propylene glycol to the glycerol is 1: 0.75.

the amount of the thickener is 0.2 percent of the total weight of the solvent. The thickening agent is hydroxyethyl cellulose. The preservative is chlorphenesin.

The solvent is water.

The frequency of the step (1) during mixing and stirring is 30 Hz; and (3) the frequency during homogenizing in the step (3) is 60 Hz.

The aperture of the filter in the step (4) is 100 meshes.

Example 2

(1) mixing and stirring the humectant and the thickener, adding the mixture into the solvent, stirring and heating the mixture to 70 ℃, adding the preservative, the first skin conditioner, the first bactericide and the second skin conditioner, and mixing and stirring the mixture to obtain a phase A;

(2) separately mixing emulsifier, emollient, and antioxidant, stirring, heating to 70 deg.C, and adding to obtain phase B;

(3) keeping the temperature at 70 ℃, mixing the phase B and the phase A, homogenizing for 60 minutes, and cooling to obtain a premix;

In the step (3), the vacuum degree in the homogenizing process is-0.03 Mpa.

The total amount of the first skin conditioning agent and the second skin conditioning agent is 1 percent of the weight of the solvent.

The first skin conditioner is allantoin, and the second skin conditioner is the combination of picrorhiza rhizome, borneol, catechu, berberine hydrochloride, fragrant ink, artificial musk, artificial bezoar, bear gall powder and ox bile; the weight ratio of the first skin conditioning agent to the second skin conditioning agent is 0.01: 1. the weight ratio of rhizoma picrorhizae, borneol, catechu, berberine hydrochloride, fragrant ink, artificial musk, artificial bezoar, bear gall powder and ox bile is 1: 0.01: 0.5: 0.01: 1.5: 0.01: 0.01: 0.1: 1.

the dosage of the emulsifier is 10 percent of the total weight of the solvent. The emulsifier is selected from the group consisting of fatty acid esters, polyglyceryl-3-methylglucidistearate, cetearyl alcohol. The weight ratio of the fatty acid ester series, the polyglycerol-3 methyl glucose distearate and the cetearyl alcohol is 1: 0.5: 1.5. polyglyceryl-3 methylglucose distearate brand as TEGOCARE 450; the fatty acid ester series was purchased from emulsifier model a 170.

The amount of the emollient is 10% of the total weight of the solvent. The emollient is selected from the group consisting of cyclosiloxane complex, isooctyl palmitate, ethylhexyl cetyl, petrolatum. The weight ratio of the cyclosiloxane compound to the isooctyl palmitate to the ethylhexyl cetyl alcohol to the petrolatum is 1: 1: 0.5: 0.5. the cyclosiloxane compound is cyclopentadimethylsiloxane and cyclohexasiloxane; the weight ratio of the cyclopentadimethylsiloxane to the cyclohexasiloxane is 1: 0.5.

the addition amount of the humectant is 0.5 percent of the dosage of the solvent. The humectant is a combination of 1, 3-propylene glycol and glycerol; the weight ratio of the 1, 3-propylene glycol to the glycerol is 1: 0.5.

the amount of the thickener is 0.1 percent of the total weight of the solvent. The thickening agent is hydroxyethyl cellulose. The preservative is chlorphenesin.

The solvent is water.

The aperture of the filter in the step (4) is 100 meshes.

Example 3

(1) mixing and stirring the humectant and the thickener, adding the mixture into the solvent, stirring and heating the mixture to 90 ℃, adding the preservative, the first skin conditioner, the first bactericide and the second skin conditioner, and mixing and stirring the mixture to obtain a phase A;

(2) separately mixing emulsifier, emollient, and antioxidant, stirring, heating to 90 deg.C, and adding to obtain phase B;

(3) keeping the temperature at 90 ℃, mixing the phase B and the phase A, homogenizing for 30 minutes, and cooling to obtain a premix;

In the step (3), the vacuum degree in the homogenizing process is-0.03 Mpa.

The total amount of the first skin conditioning agent and the second skin conditioning agent is 5% of the weight of the solvent.

The first skin conditioner is allantoin, and the second skin conditioner is the combination of picrorhiza rhizome, borneol, catechu, berberine hydrochloride, fragrant ink, artificial musk, artificial bezoar, bear gall powder and ox bile; the weight ratio of the first skin conditioning agent to the second skin conditioning agent is 0.1: 1. the weight ratio of rhizoma picrorhizae, borneol, catechu, berberine hydrochloride, fragrant ink, artificial musk, artificial bezoar, bear gall powder and ox bile is 1: 0.1: 1.5: 0.1: 2.5: 0.1: 0.1: 0.5: 2.

the dosage of the emulsifier is 20 percent of the total weight of the solvent. The emulsifier is selected from the group consisting of fatty acid esters, polyglyceryl-3-methylglucidistearate, cetearyl alcohol. The weight ratio of the fatty acid ester series, the polyglycerol-3 methyl glucose distearate and the cetearyl alcohol is 1: 1.5: 2.5. polyglyceryl-3 methylglucose distearate brand as TEGOCARE 450; the fatty acid ester series comprise polyethylene glycol stearate and glycerol monostearate, wherein the weight ratio of the polyethylene glycol stearate to the glycerol fatty acid ester is 1: 1. the polyethylene glycol stearate is PEG-100 stearate with model of SG-6, and is purchased from Ghan Hua Zhixiang scientific biotechnology, Inc.; the glycerol monostearate was purchased from south china jiuze chemical company under the code GMS.

The amount of the emollient is 15% of the total weight of the solvent. The emollient is selected from the group consisting of cyclosiloxane complex, isooctyl palmitate, ethylhexyl cetyl, petrolatum. The weight ratio of the cyclosiloxane compound to the isooctyl palmitate to the ethylhexyl cetyl alcohol to the petrolatum is 1: 1.5: 1.5: 1. the cyclosiloxane compound is cyclopentadimethylsiloxane and cyclohexasiloxane; the weight ratio of the cyclopentadimethylsiloxane to the cyclohexasiloxane is 1: 3.

the addition amount of the humectant is 1 percent of the dosage of the solvent. The humectant is a combination of 1, 3-propylene glycol and glycerol; the weight ratio of the 1, 3-propylene glycol to the glycerol is 1: 1.

the amount of the thickener is 1 percent of the total weight of the solvent. The thickening agent is hydroxyethyl cellulose. The preservative is chlorphenesin.

The solvent is water.

The aperture of the filter in the step (4) is 100 meshes.

Example 4

(1) mixing and stirring the humectant and the thickener, adding the mixture into the solvent, stirring and heating the mixture to 60 ℃, adding the preservative, the first skin conditioner, the first bactericide and the second skin conditioner, and mixing and stirring the mixture to obtain a phase A;

(2) separately mixing emulsifier, emollient, and antioxidant, stirring, heating to 60 deg.C, and adding to obtain phase B;

(3) keeping the temperature at 60 ℃, mixing the phase B and the phase A, homogenizing for 5 minutes, and cooling to obtain a premix;

In the step (3), the vacuum degree in the homogenizing process is-0.03 Mpa.

The solvent is water.

The aperture of the filter in the step (4) is 100 meshes.

Example 5

(1) mixing and stirring the humectant and the thickener, adding the mixture into the solvent, stirring and heating the mixture to 50 ℃, adding the preservative, the first skin conditioner, the first bactericide and the second skin conditioner, and mixing and stirring the mixture to obtain a phase A;

(2) separately mixing emulsifier, emollient, and antioxidant, stirring, heating to 50 deg.C, and adding to obtain phase B;

(3) keeping the temperature at 50 ℃, mixing the phase B and the phase A, homogenizing for 5 minutes, and cooling to obtain a premix;

In the step (3), the vacuum degree in the homogenizing process is-0.03 Mpa.

The solvent is water.

The aperture of the filter in the step (4) is 100 meshes.

Example 6

(3) keeping the temperature at 40 ℃, mixing the phase B and the phase A, homogenizing for 5 minutes, and cooling to obtain a premix;

In the step (3), the vacuum degree in the homogenizing process is-0.03 Mpa.

The solvent is water.

The aperture of the filter in the step (4) is 100 meshes.

Example 7

(1) mixing and stirring the humectant and the thickener, adding the mixture into the solvent, stirring and heating the mixture to 80 ℃, adding the preservative, the first bactericide and the second skin conditioner, and mixing and stirring the mixture to obtain a phase A;

In the step (3), the vacuum degree in the homogenizing process is-0.03 Mpa.

The total amount of the second skin conditioning agent was 2.5% by weight of the solvent.

The second skin conditioner is a combination of rhizoma picrorhizae, Borneolum Syntheticum, Catechu, berberine hydrochloride, XIANGMO, artificial Moschus, artificial calculus bovis, fel Ursi powder, and bilis Bovina; the weight ratio of rhizoma picrorhizae, borneol, catechu, berberine hydrochloride, fragrant ink, artificial musk, artificial bezoar, bear gall powder and ox bile is 1: 0.06: 1: 0.065: 2: 0.05: 0.05: 0.2: 1.6.

The solvent is water.

The aperture of the filter in the step (4) is 100 meshes.

Example 8

In the step (3), the vacuum degree in the homogenizing process is-0.03 Mpa.

The first skin conditioner is allantoin, and the second skin conditioner is the combination of picrorhiza rhizome, borneol, catechu, berberine hydrochloride, fragrant ink, artificial musk, artificial bezoar, bear gall powder and ox bile; the weight ratio of the first skin conditioning agent to the second skin conditioning agent is 0.005: 1. the weight ratio of rhizoma picrorhizae, borneol, catechu, berberine hydrochloride, fragrant ink, artificial musk, artificial bezoar, bear gall powder and ox bile is 1: 0.06: 1: 0.065: 2: 0.05: 0.05: 0.2: 1.6.

The solvent is water.

The aperture of the filter in the step (4) is 100 meshes.

Example 9

In the step (3), the vacuum degree in the homogenizing process is-0.03 Mpa.

the dosage of the emulsifier is 15 percent of the total weight of the solvent. The emulsifier is selected from the group consisting of polyglyceryl-3 methyl glucose distearate, cetostearyl alcohol. The weight ratio of the polyglycerol-3-methylglucose distearate to the cetearyl alcohol is 1: 2. polyglyceryl-3 methylglucose distearate brand as TEGOCARE 450;

The solvent is water.

The aperture of the filter in the step (4) is 100 meshes.

Example 10

In the step (3), the vacuum degree in the homogenizing process is-0.03 Mpa.

The amount of the emollient is 12% of the total weight of the solvent. The emollient is selected from the group consisting of isooctyl palmitate, ethylhexyl cetyl, petrolatum. The weight ratio of isooctyl palmitate, ethylhexyl cetyl alcohol and petrolatum is 1.3: 1: 0.8.

The solvent is water.

The aperture of the filter in the step (4) is 100 meshes.

Performance testing

1. And (3) testing the itching relieving effect: the test was carried out by taking 50 mice each half of which was divided into 10 groups on a random average, and testing 5 mice per group while using physiological saline as a control group 1 and without any treatment as a control group 2, and applying the product obtained in example. The right hind instep of the mouse was dehaired and the instep shaved evenly with coarse sandpaper, all mice were treated the same way, and control group 2 was shaved without the instep shaved. The antibacterial and anti-inflammatory ointment and the control group of the embodiment are respectively smeared on the dorsum of foot wound of the mouse, and the film is uniformly and thinly coated, and each small white water is smeared for the same time in the morning and at night. And are recorded as follows, and table 1 is recorded:

on the coating day: the time for the first round of the mice to lick the right paw after rub was recorded for each group and the average time was calculated.

After three days, observation: the frequency of right paw licking in the mice was recorded every two hours.

TABLE 1 antipruritic Effect test results

2. And (3) testing heat resistance stability: keeping the temperature at 40 +/-1 ℃ for 24 hours, after the temperature is recovered to the room temperature, observing whether the oil-water separation phenomenon exists, and recording as qualified, otherwise, recording as unqualified; and record table 2.

3. Cold resistance stability test: keeping at-10 ℃ for 24 hours, and after the room temperature is recovered, observing the appearance difference between the test sample and the test sample; if no change exists, marking as qualified, otherwise marking as unqualified; and record table 2.

Table 2 stability test results

4. Now, a plurality of typical cases are listed, which illustrate that the antibacterial and anti-inflammatory ointment prepared according to the preparation method of the invention has very excellent and rapid effect of relieving itching.

Case 1: wangzhi, 28 years old and with folliculitis on the back, the antibacterial and anti-inflammatory ointment prepared by the preparation method of example 2 is uniformly applied to the area with folliculitis, and the area is cured in the morning and evening and after 3 days without scars.

Case 2: liu Yi, 40 years old, neck with allergic dermatitis and accompanied with itching and red spots, and the antibacterial and anti-inflammatory ointment prepared by the preparation method of example 2 is uniformly applied to the areas with itching and red spots, and is cured once in the morning and at night after one day without leaving any scars.

Case 3: in Sunzhi, the area of 21 years old, such as neck, chest, abdomen and arms, suffers from severe allergic dermatitis accompanied by red spots of pruritus, the antibacterial and anti-inflammatory ointment prepared by the preparation method described in example 6 is uniformly applied to the area where the pruritus and the red spots appear, and is cured once in the morning and at night and after 7 days.

The foregoing examples are merely illustrative and serve to explain some of the features of the method of the present invention. The appended claims are intended to claim as broad a scope as is contemplated, and the examples presented herein are merely illustrative of selected implementations in accordance with all possible combinations of examples. Accordingly, it is applicants' intention that the appended claims are not to be limited by the choice of examples illustrating features of the invention. Also, where numerical ranges are used in the claims, subranges therein are included, and variations in these ranges are also to be construed as possible being covered by the appended claims.

Claims

1. The preparation method of the antibacterial and anti-inflammatory ointment is characterized by at least comprising the following steps:

2. The method for preparing antibacterial and anti-inflammatory ointment according to claim 1, wherein in the step (3), the vacuum degree in the homogenization process is-0.03 to 0.01 MPa.

3. A method for preparing a bacteriostatic and anti-inflammatory ointment according to claim 1, wherein the first skin conditioning agent is at least one selected from allantoin, glyceryl caprylate, hexyldecanol, bisabolol, cetyl N-palmitoyl hydroxyproline, stearic acid; the second skin conditioner is selected from at least one or more of radix Angelicae sinensis, rhizoma Ligustici Chuanxiong, Saviae Miltiorrhizae radix, rhizoma picrorhizae, Borneolum Syntheticum, Catechu, berberine hydrochloride, Chinese ink, artificial Moschus, artificial calculus bovis, fel Ursi powder, and bilis Bovina.

4. A method of preparing a bacteriostatic and anti-inflammatory ointment according to any one of claims 1 to 3 wherein the first skin conditioning agent is allantoin; the second skin conditioner is selected from at least one or more of rhizoma picrorhizae, Borneolum Syntheticum, Catechu, berberine hydrochloride, XIANGMO, artificial Moschus, artificial calculus bovis, fel Ursi powder, and bilis Bovina; the weight ratio of the first skin conditioning agent to the second skin conditioning agent is (0.01-0.1): 1.

5. a method for preparing a bacteriostatic and anti-inflammatory ointment according to claim 1 or 2, wherein the emulsifier is selected from one or more of fatty acid ester series, polyglycerol-3-methylglucdistearate, cetearyl alcohol, glycerol, polyoxyl stearate, polyoxyethylene lauryl ether, alkyl glycoside, caprylic/capric triglyceride.

6. The method for preparing antibacterial and anti-inflammatory ointment according to claim 5, wherein the fatty acid ester series comprises at least one of polyethylene glycol stearate, glycerin fatty acid ester, and propylene glycol fatty acid ester; the saponification value of the polyethylene glycol stearate is 85-120 mgKOH/g; the saponification value of the glycerol fatty acid ester is 160-220 mgKOH/g.

7. A method of preparing a bacteriostatic and anti-inflammatory ointment according to claim 1 or 2 wherein the emollient is selected from one or more of cyclosiloxane complex, isooctyl palmitate, ethylhexyl cetyl ester, petrolatum, beeswax, caprylic/capric triglyceride.

8. The method for preparing antibacterial and anti-inflammatory ointment according to claim 1 or 2, wherein the frequency of mixing and stirring in step (1) is 15-45 Hz; the frequency of the homogenization in the step (3) is 30-120 Hz.

9. The method for preparing antibacterial and anti-inflammatory ointment according to claim 1 or 2, wherein the pore size during filtration in step (4) is 50-200 mesh.

10. The application of the antibacterial and anti-inflammatory ointment is characterized in that the antibacterial and anti-inflammatory ointment is prepared by the preparation method of any one of claims 1 to 9; the antibacterial and anti-inflammatory ointment is applied to at least one of allergic skin, erythra skin, acne skin and scalp itch.