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CN111631132A - Breeding method of herbicide-resistant dry-planting rice variety - Google Patents

Breeding method of herbicide-resistant dry-planting rice variety Download PDF

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Publication number
CN111631132A
CN111631132A CN202010685547.5A CN202010685547A CN111631132A CN 111631132 A CN111631132 A CN 111631132A CN 202010685547 A CN202010685547 A CN 202010685547A CN 111631132 A CN111631132 A CN 111631132A
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China
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herbicide
rice
breeding
dry
resistant
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Inventor
谢树鹏
刘立超
门龙楠
魏中华
孙中华
宗天鹏
张广彬
景玉良
刘宝海
聂守军
高世伟
刘晴
刘宇强
马成
常汇琳
符强
冯鑫
张起昌
米刚
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Suihua Branch Of Heilongjiang Academy Of Agricultural Sciences
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Suihua Branch Of Heilongjiang Academy Of Agricultural Sciences
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/02Methods or apparatus for hybridisation; Artificial pollination ; Fertility
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01HNEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
    • A01H1/00Processes for modifying genotypes ; Plants characterised by associated natural traits
    • A01H1/04Processes of selection involving genotypic or phenotypic markers; Methods of using phenotypic markers for selection

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Botany (AREA)
  • Developmental Biology & Embryology (AREA)
  • Environmental Sciences (AREA)
  • Pretreatment Of Seeds And Plants (AREA)

Abstract

A method for breeding herbicide-resistant dry-breeding rice varieties relates to a method for breeding rice varieties. The invention aims to solve the problem that weeding is difficult in field management of the existing drought-enduring rice. The breeding method comprises the following steps: firstly, hybridizing drought-enduring rice A serving as a female parent and Jie tian rice serving as a male parent; secondly, spraying and applying a herbicide for screening; third, herbicide resistant line F1Hybridizing the female parent B and the male parent A; fourthly, spraying and applying herbicide for screening; five, herbicide resistant line F2Hybridizing the female parent B and the male parent A; sixthly, spraying and applying a herbicide for screening; seventhly, drought tolerance treatment; eight, drought-enduring strain F with herbicide resistance3‑BC1And carrying out drought-enduring treatment and planting on the initial generation to obtain the herbicide-resistant dry-planting rice variety. The advantages are that: not only provides a foundation for selecting dry-planted rice varieties, but also solves the problem of weed control after dry planting of rice. The invention is mainly used for breeding herbicide-resistant dry seedsA rice variety.

Description

Breeding method of herbicide-resistant dry-planting rice variety
Technical Field
The invention relates to a breeding method of rice varieties.
Background
With the increasing shortage of water resources and the increasing severity of drought, the shortage of water resources is becoming an important factor for restricting the agricultural development in China. The water consumption of the dry planting of the rice is only 20 percent of that of the rice transplanting and cultivating, and the threat of water resource shortage in China can be relieved by the dry planting of the rice. The drought-resistant cultivated rice variety is cultivated and the drought-resistant cultivation of the rice is realized, so that water resources can be saved to a greater extent, and the production is stabilized, the energy is saved and the environmental pollution is reduced. And compared with rice transplanting, the rice dry planting has some new problems. The more outstanding problems are: the seedlings are difficult to be sowed at one time to be full, neat and strong; easily breeding weeds, and having various types and serious harm. The selection of a suitable variety is a prerequisite for success or failure of dry-breeding of rice.
Disclosure of Invention
The invention aims to solve the problem of difficulty in weeding in field management of the conventional drought-resistant rice, and provides a method for breeding a herbicide-resistant drought-planted rice variety.
A method for breeding herbicide-resistant dry-breeding rice varieties is completed by the following steps:
firstly, selecting drought-resistant rice A as female parent and Jie Tian rice as male parent, and adopting glume cutting method to make hybridization under the condition of dry seed selection and breeding to obtain F1Seed generation;
II, mixing F1Sowing seeds under the condition of dry seed breeding, spraying herbicide at the 2.5 leaf age of seedling stage for screening, and eliminating the strain F with gradually withered seedlings after spraying herbicide1-A, obtaining a herbicide-resistant strain F1-B;
Thirdly, selecting herbicide resistant strain F1Hybridizing the-B serving as a female parent and the drought-resistant rice A serving as a male parent by adopting a glume shearing method under the condition of dry seed breeding to obtain F2Seed generation;
fourthly, F is mixed2Sowing seeds under the condition of dry seed breeding, spraying herbicide at the 2.5 leaf age of seedling stage for screening, and eliminating the strain F with gradually withered seedlings after spraying herbicide2-A, obtaining a herbicide-resistant strain F2-B;
Fifthly, selecting herbicide-resistant strain F2Hybridizing the-B serving as a female parent and the drought-resistant rice A serving as a male parent by adopting a glume shearing method under the condition of dry seed breeding to obtain F3Seed generation;
sixthly, F3The seeds are sown under the condition of dry seed selection and in the seedling stageSpraying herbicide at the age of 2.5 leaves for screening, and eliminating the strain F with gradually withered seedlings after spraying herbicide3-A, obtaining a herbicide-resistant strain F3-B;
Seventh, the herbicide resistant strain F3Sowing the seeds under the condition of dry seed breeding, spraying herbicide for screening at the 2.5 leaf age of seedling stage, and carrying out drought-enduring treatment at the booting stage and the grouting stage to obtain a herbicide-resistant drought-enduring strain F3-BC1
Eight, drought-enduring strain F with herbicide resistance3-BC1And (5) planting the rice for the first generation according to the selection and breeding conditions in the step seven until the consistency of the population in the line is stable, thereby obtaining the herbicide-resistant dry-planting rice variety.
The principle and the advantages of the invention are as follows: the tolerance of the Jieshan rice to herbicide is nearly ten thousand times higher than that of common rice, and the method utilizes the Jieshan rice breeding to obtain the herbicide-resistant dry-planting rice variety, thereby not only providing a foundation for selecting the dry-planting rice variety, but also solving the problem of weed control after the dry-planting of the rice.
Detailed Description
The first embodiment is as follows: the embodiment is a breeding method of herbicide-resistant dry-breeding rice varieties, which is completed by the following steps:
firstly, selecting drought-resistant rice A as female parent and Jie Tian rice as male parent, and adopting glume cutting method to make hybridization under the condition of dry seed selection and breeding to obtain F1Seed generation;
II, mixing F1Sowing seeds under the condition of dry seed breeding, spraying herbicide at the 2.5 leaf age of seedling stage for screening, and eliminating the strain F with gradually withered seedlings after spraying herbicide1-A, obtaining a herbicide-resistant strain F1-B;
Thirdly, selecting herbicide resistant strain F1Hybridizing the-B serving as a female parent and the drought-resistant rice A serving as a male parent by adopting a glume shearing method under the condition of dry seed breeding to obtain F2Seed generation;
fourthly, F is mixed2Sowing seeds under the condition of dry seed breeding, spraying herbicide at the 2.5 leaf age of seedling stage for screening, and eliminating the strain F with gradually withered seedlings after spraying herbicide2-ATo obtain a herbicide-resistant strain F2-B;
Fifthly, selecting herbicide-resistant strain F2Hybridizing the-B serving as a female parent and the drought-resistant rice A serving as a male parent by adopting a glume shearing method under the condition of dry seed breeding to obtain F3Seed generation;
sixthly, F3Sowing seeds under the condition of dry seed breeding, spraying herbicide at the 2.5 leaf age of seedling stage for screening, and eliminating the strain F with gradually withered seedlings after spraying herbicide3-A, obtaining a herbicide-resistant strain F3-B;
Seventh, the herbicide resistant strain F3Sowing the seeds under the condition of dry seed breeding, spraying herbicide for screening at the 2.5 leaf age of seedling stage, and carrying out drought-enduring treatment at the booting stage and the grouting stage to obtain a herbicide-resistant drought-enduring strain F3-BC1
Eight, drought-enduring strain F with herbicide resistance3-BC1And (5) planting the rice for the first generation according to the selection and breeding conditions in the step seven until the consistency of the population in the line is stable, thereby obtaining the herbicide-resistant dry-planting rice variety.
Shenzhen, a research institute for molecular design and breeding of crops, Daizhibo team, in 2010, 12 tons of seeds subjected to chemical mutagenesis were collected from 60 mu of test field, 2 tons of seeds were taken out and planted in the field, herbicide (mebendazole hydrochloride) was sprayed at the 3-leaf stage of seedlings, and almost all seedlings were killed by the herbicide, but only one plant was naturally safe. The seedling is protected in emphasis, blossoms and fruits, and has herbicide resistance after detection and field test. The tolerance of the seeds (Jie tian rice) of the rice to herbicide (methyl imidazole hydrochloride) is nearly ten thousand times higher than that of common rice. The method for breeding the new herbicide-resistant dry-planting rice variety by utilizing the clean-field rice not only provides a basis for selecting the dry-planting rice variety, but also can solve the problem of weed control after the dry-planting of the rice.
The second embodiment is as follows: the present embodiment differs from the first embodiment in that: the drought-enduring rice A in the first, third and fifth steps is selected according to the following steps:
collecting the existing high-quality drought-enduring rice seeds and high-quality drought-enduring rice seeds, sowing under the condition of drought seed breeding, carrying out drought-enduring treatment, and finally selecting the variety with the maturity period of the clean field rice of +/-10 days, strong stalk and highest average single plant yield as drought-enduring rice A.
The rest is the same as the first embodiment.
The high-quality drought-enduring rice seeds of the embodiment are the Amblyseius japonica 304, the Amblyseius japonica 26, the Amblyseius rice No. 3 and the Longyang 11; the high-quality dry rice seeds are original dry rice No. 3, dry rice No. 297 and dry rice No. 9.
The Suizhijing 304, the Suizhijing 26 and the dry rice 297 are determined to be taken as drought-resistant rice A by screening.
The third concrete implementation mode: the present embodiment differs from the first or second embodiment in that: the dry breeding method specifically comprises the following operations: firstly, applying a chemical fertilizer in one step before rotary tillage, wherein the chemical fertilizer comprises diammonium phosphate, urea and potassium sulfate, the application amount of the diammonium phosphate is 15 kg/mu, the application amount of the urea is 10 kg/mu, and the application amount of the potassium sulfate is 5 kg/mu; secondly, rotating the ground in the middle ten days of 4 months every year, wherein the depth is 15cm, fishing out, and compacting for 2 times before sowing; thirdly, sowing dry seeds in rows at the beginning of 5 months every year, wherein the sowing depth is 2 cm-3 cm, the row spacing is 25cm, the sowing quantity is 150 kg/hectare, and the seeds are compacted for 1 time after sowing. The others are the same as in the first or second embodiment.
The fourth concrete implementation mode: the difference between this embodiment and one of the first to third embodiments is as follows: the herbicide is imazapic. The others are the same as the first to third embodiments.
The mebendazole hydrochloride is a novel, broad-spectrum and efficient herbicide special for peanut fields, has strong internal absorption, has good control effect on monocotyledonous and dicotyledonous weeds, can well kill overground parts of the weeds, and has strong control effect on roots and underground stems of the weeds.
The fifth concrete implementation mode: the difference between this embodiment and one of the first to fourth embodiments is: the spraying amount of the herbicide is 20 mL/mu to 30 mL/mu. The rest is the same as the first to fourth embodiments.
The spraying amount of the herbicide is strictly controlled, and the conditions that the herbicide is too little, the weeding effect cannot be achieved, and the herbicide is too much, so that all the rice dies are avoided.
The sixth specific implementation mode: the difference between this embodiment and one of the first to fifth embodiments is as follows: the drought tolerance treatment specifically comprises the following operations: the growth period of the rice is mainly natural rainfall, no additional artificial irrigation is needed, drought stress is carried out in the booting period and the grouting period until the leaves of the rice wither, rehydration is carried out after 3 days, and the water content of the soil accounting for 20cm is guaranteed to be 40% -45% after rehydration. The rest is the same as the first to fifth embodiments.
The seventh embodiment: the present embodiment differs from the sixth embodiment in that: the drought stress is the control of rainfall by covering a white plastic rain-proof film. The rest is the same as the sixth embodiment.
The specific implementation mode is eight: the seventh embodiment is different from the seventh embodiment in that: the light transmittance of the white plastic rainproof film is more than 95%. The rest is the same as the seventh embodiment.
The invention is not limited to the above embodiments, and one or a combination of several embodiments may also achieve the object of the invention.
The following tests were carried out to confirm the effects of the present invention
Example 1: a method for breeding herbicide-resistant dry-breeding rice varieties is completed by the following steps:
firstly, selecting drought-resistant rice A: collecting the existing high-quality drought-enduring rice seeds and high-quality drought-enduring rice seeds, sowing under the condition of drought seed breeding, carrying out drought-enduring treatment, and finally selecting the variety with the maturity period of the clean field rice of +/-10 d, strong stalk and highest average single plant yield as drought-enduring rice A; the drought-resistant rice A is dry rice 297; the high-quality drought-enduring rice seeds are the Amblyseius japonica 304, the Amblyseius japonica 26, the Amblyseius rice No. 3 and the Longyang 11; the high-quality dry rice seeds are original dry rice No. 3, dry rice No. 297 and dry rice No. 9;
secondly, selecting dry rice 297 as a female parent and Jie tian rice as a male parent, and hybridizing under the condition of dry seed breeding by adopting a glume shearing method to obtain F1Seed generation;
thirdly, mixing F1The seeds are sown under the condition of dry seed selection and breedingSpraying herbicide for screening at the 2.5 leaf age of the seedling stage, and eliminating the strain F with gradually withered seedlings after spraying herbicide1-A, obtaining a herbicide-resistant strain F1-B; the herbicide is imazapic; the spraying amount of the herbicide is 25 mL/mu;
fourthly, selecting herbicide-resistant strain F1Hybridizing the-B serving as a female parent and the dry rice 297 serving as a male parent by adopting a glume shearing method under the condition of dry seed breeding to obtain F2Seed generation;
fifthly, F is2Sowing seeds under the condition of dry seed breeding, spraying herbicide at the 2.5 leaf age of seedling stage for screening, and eliminating the strain F with gradually withered seedlings after spraying herbicide2-A, obtaining a herbicide-resistant strain F2-B; the herbicide is imazapic; the spraying amount of the herbicide is 25 mL/mu;
sixthly, selecting herbicide-resistant strain F2Hybridizing the-B serving as a female parent and the dry rice 297 serving as a male parent by adopting a glume shearing method under the condition of dry seed breeding to obtain F3Seed generation;
seventhly, F3Sowing seeds under the condition of dry seed breeding, spraying herbicide at the 2.5 leaf age of seedling stage for screening, and eliminating the strain F with gradually withered seedlings after spraying herbicide3-A, obtaining a herbicide-resistant strain F3-B; the herbicide is imazapic; the spraying amount of the herbicide is 25 mL/mu;
eighthly, herbicide-resistant strain F3Sowing the seeds under the condition of dry seed breeding, spraying herbicide for screening at the 2.5 leaf age of seedling stage, and carrying out drought-enduring treatment at the booting stage and the grouting stage to obtain a herbicide-resistant drought-enduring strain F3-BC1(ii) a The herbicide is imazapic; the spraying amount of the herbicide is 25 mL/mu;
ninthly, drought-enduring strain F with herbicide resistance3-BC1Planting 3 generations for the first generation according to the selection and breeding conditions in the step eight, namely a 4 th generation herbicide-resistant drought-enduring strain F3-BC4Herbicide resistant drought tolerant lines F3-BC4The consistency of the population in the strain is stable, and the herbicide-resistant dry-planting rice variety is obtained.
The dry breeding operation described in example 1 is as follows: firstly, applying a chemical fertilizer in one step before rotary tillage, wherein the chemical fertilizer comprises diammonium phosphate, urea and potassium sulfate, the application amount of the diammonium phosphate is 15 kg/mu, the application amount of the urea is 10 kg/mu, and the application amount of the potassium sulfate is 5 kg/mu; secondly, rotating the ground in the middle ten days of 4 months every year, wherein the depth is 15cm, fishing out, and compacting for 2 times before sowing; thirdly, sowing dry seeds in rows at the beginning of 5 months every year, wherein the sowing depth is 2 cm-3 cm, the row spacing is 25cm, the sowing quantity is 150 kg/hectare, and the seeds are compacted for 1 time after sowing.
The drought tolerance treatment described in example 1 was carried out as follows: the growth period of the rice is mainly natural rainfall without additional artificial irrigation, drought stress is carried out in the booting period and the grouting period until the leaves of the rice wither, rehydration is carried out after 3 days, and the water content of the soil accounting for 20cm is ensured to be 43% after rehydration; the drought stress is that the rainfall is controlled by covering a white plastic rainproof film; the light transmittance of the white plastic rainproof film is 98%.
The 4 th generation herbicide-resistant drought-enduring strain F can be found by statistics3-BC4The average yield per mu of the dry rice is 366.50kg, and the average yield per mu of the dry rice 297 is 360.44 kg; the average acre yield of the herbicide-resistant dry-planting rice variety screened by the invention is slightly higher than that of dry-resistant rice A (dry rice 297).

Claims (8)

1. A method for breeding herbicide-resistant dry-breeding rice varieties is characterized by comprising the following steps:
firstly, selecting drought-resistant rice A as female parent and Jie Tian rice as male parent, and adopting glume cutting method to make hybridization under the condition of dry seed selection and breeding to obtain F1Seed generation;
II, mixing F1Sowing seeds under the condition of dry seed breeding, spraying herbicide at the 2.5 leaf age of seedling stage for screening, and eliminating the strain F with gradually withered seedlings after spraying herbicide1-A, obtaining a herbicide-resistant strain F1-B;
Thirdly, selecting herbicide resistant strain F1Hybridizing the-B serving as a female parent and the drought-resistant rice A serving as a male parent by adopting a glume shearing method under the condition of dry seed breeding to obtain F2Seed generation;
fourthly, F is mixed2Sowing seeds under the condition of dry seed breeding, spraying herbicide at the 2.5 leaf age of seedling stage for screening, and eliminating the strain F with gradually withered seedlings after spraying herbicide2-A, obtaining a herbicide-resistant strain F2-B;
Fifthly, selecting herbicide-resistant strain F2Hybridizing the-B serving as a female parent and the drought-resistant rice A serving as a male parent by adopting a glume shearing method under the condition of dry seed breeding to obtain F3Seed generation;
sixthly, F3Sowing seeds under the condition of dry seed breeding, spraying herbicide at the 2.5 leaf age of seedling stage for screening, and eliminating the strain F with gradually withered seedlings after spraying herbicide3-A, obtaining a herbicide-resistant strain F3-B;
Seventh, the herbicide resistant strain F3Sowing the seeds under the condition of dry seed breeding, spraying herbicide for screening at the 2.5 leaf age of seedling stage, and carrying out drought-enduring treatment at the booting stage and the grouting stage to obtain a herbicide-resistant drought-enduring strain F3-BC1
Eight, drought-enduring strain F with herbicide resistance3-BC1And (5) planting the rice for the first generation according to the selection and breeding conditions in the step seven until the consistency of the population in the line is stable, thereby obtaining the herbicide-resistant dry-planting rice variety.
2. The method for breeding the herbicide-resistant dry-breeding rice variety according to claim 1, wherein the drought-tolerant rice A in the first, third and fifth steps is selected according to the following steps:
collecting the existing high-quality drought-enduring rice seeds and high-quality drought-enduring rice seeds, sowing under the condition of drought seed breeding, carrying out drought-enduring treatment, and finally selecting the variety with the maturity period of the clean field rice of +/-10 days, strong stalk and highest average single plant yield as drought-enduring rice A.
3. The method for breeding the herbicide-resistant dry-breeding rice variety according to claim 1 or 2, wherein the dry-breeding is specifically performed as follows: firstly, applying a chemical fertilizer in one step before rotary tillage, wherein the chemical fertilizer comprises diammonium phosphate, urea and potassium sulfate, the application amount of the diammonium phosphate is 15 kg/mu, the application amount of the urea is 10 kg/mu, and the application amount of the potassium sulfate is 5 kg/mu; secondly, rotating the ground in the middle ten days of 4 months every year, wherein the depth is 15cm, fishing out, and compacting for 2 times before sowing; thirdly, sowing dry seeds in rows at the beginning of 5 months every year, wherein the sowing depth is 2 cm-3 cm, the row spacing is 25cm, the sowing quantity is 150 kg/hectare, and the seeds are compacted for 1 time after sowing.
4. The method for breeding the herbicide-resistant dry-land rice variety as claimed in claim 1, wherein the herbicide is imazapic.
5. The method for breeding the herbicide-resistant dry-land rice variety as claimed in claim 1 or 4, wherein the spraying amount of the herbicide is 20-30 mL/acre.
6. The method for breeding the herbicide-resistant dry-land rice variety according to claim 1 or 2, wherein the drought-enduring treatment is specifically performed as follows: the growth period of the rice is mainly natural rainfall, no additional artificial irrigation is needed, drought stress is carried out in the booting period and the grouting period until the leaves of the rice wither, rehydration is carried out after 3 days, and the water content of the soil accounting for 20cm is guaranteed to be 40% -45% after rehydration.
7. The method for breeding the herbicide-resistant dry-breeding rice variety as claimed in claim 6, wherein the drought stress is controlling the rainfall by covering a white plastic rain-proof film.
8. The method for breeding the herbicide-resistant dry-planting rice variety as claimed in claim 7, wherein the light transmittance of the white plastic rain-proof film is more than 95%.
CN202010685547.5A 2020-07-16 2020-07-16 Breeding method of herbicide-resistant dry-planting rice variety Pending CN111631132A (en)

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Application publication date: 20200908