CN111575332A - Preparation method of cerebroprotein hydrolysate - Google Patents

Abstract

The present invention relates to a preparation method of cerebroprotein hydrolysate, comprising the following steps: homogenizing brain tissue to obtain a homogenate solution; subjecting the homogenate solution to enzymolysis treatment to obtain an enzymatic solution; Add a sedimentation agent, remove the oil layer on the surface after standing for stratification, and collect the sedimentation supernatant; add a flocculant to the sedimentation supernatant, centrifuge, and collect the centrifugal supernatant; in the centrifugal supernatant Activated carbon is added, the temperature is kept at 50 DEG C to 70 DEG C for 10 minutes to 30 minutes, and the filtrate is collected after filtration to obtain the cerebroprotein hydrolyzate. The preparation method of the invention does not use an organic solvent, the process is simple and feasible, the energy consumption is low, and waste residue treatment is also considered, which is very environmentally friendly, conforms to the concept of green production, and is convenient for workers to operate and popularized for large-scale industrial production.

Description

Preparation method of cerebral protein hydrolyzate

technical field

The invention relates to the technical field of medicine, in particular to a preparation method of a cerebroprotein hydrolysate.

Background technique

Cerebroprotein hydrolysate is a mixture of peptides, various amino acids and trace elements such as selenium, potassium, phosphorus and magnesium obtained by hydrolysis of animal brain tissue. It can act on human nerve tissue in various ways, can provide amino acids for neuron repair, nourish nerve cells, regulate and improve neuron metabolism, promote synapse formation, induce neuron differentiation, and further protect nerve cells from Various ischemia and neurotoxin damage. Clinically, it can be used in acute cerebral infarction, brain atrophy, neonatal hypoxic-ischemic encephalopathy, diffuse axonal injury, senile dementia, Japanese encephalitis, diabetic peripheral neuropathy, sudden deafness etc. treatment.

The preparation of cerebroprotein hydrolyzate generally goes through the steps of animal brain tissue pretreatment, cerebroprotein hydrolysis, separation and purification, raw material molding, etc. Each step has different processing methods. Therefore, a method for preparing cerebroprotein hydrolysate is often formed by combining various steps of different treatment methods. For the separation and purification steps, traditional separation and purification methods such as filtration, centrifugation, ultrafiltration, column chromatography, and electrodialysis are generally used. Raw material molding refers to the final preparation of cerebroprotein hydrolyzate into a usable product form, such as cerebroprotein hydrolyzate powder made by spray drying and heat drying, or directly prepared into further oral liquids, freeze-dried preparations, injections, etc. The current preparation method of cerebroprotein hydrolysate has the disadvantages of cumbersome steps, heavy pollution and high energy consumption.

SUMMARY OF THE INVENTION

Based on this, it is necessary to provide a preparation method of cerebroprotein hydrolysate with simple process, no pollution and low energy consumption.

A preparation method of cerebroprotein hydrolysate, comprising the following steps:

The brain tissue is homogenized to obtain a homogenate;

The homogenate is subjected to enzymolysis treatment to obtain an enzymolysis solution;

Add a sedimentation agent to the enzymatic hydrolyzate, and after standing for stratification, remove the oil layer on the surface, and collect the sedimentation supernatant;

A flocculant is added to the sedimentation supernatant, centrifuged, and the centrifugal supernatant is collected;

Activated carbon is added to the centrifugal supernatant, the temperature is kept at 50° C. to 70° C. for 10 min to 30 min, and the filtrate is collected after filtration to obtain the cerebroprotein hydrolysate.

In one embodiment, before adding the precipitation agent, the enzymatic hydrolysis solution is heated to boiling, and after adding the precipitation agent, the solution is continuously boiled for 10-20 min before standing for stratification.

In one embodiment, the settling agent is selected from one or more of talc, diatomaceous earth and calcium carbonate.

In one embodiment, the mass ratio of the homogenate to the sedimentation agent is 100:(0.5-4).

In one embodiment, the flocculant is selected from one or more of gelatin, chitosan, egg white powder and 101 fruit juice clarifying agent.

In one embodiment, the mass ratio of the sedimentation supernatant to the flocculant is 100:(0.06-0.09), and the mass ratio of the centrifugal supernatant to the activated carbon is 100:(1.5-5 ).

In one embodiment, the enzymatic hydrolysis treatment includes the following steps: mixing the homogenate with water and protease, and performing a hydrolysis reaction at 40° C.˜45° C. for 8˜24 hours.

In one embodiment, the proteases include pepsin, papain, and trypsin.

In one embodiment, the amount of pepsin added is 3% to 5% of the mass of the brain tissue, and the amount of trypsin added is 4% to 6% of the mass of the brain tissue.

In one embodiment, the brain tissue is one or more of porcine brain tissue, sheep brain tissue and bovine brain tissue.

The preparation method of cerebroprotein hydrolysate of the present invention has the following advantages:

The preparation method of the present invention is treated by adding a sedimentation agent after enzymatic hydrolysis: 1) the sedimentation time can be shortened; because the animal brain residue is particularly sticky, it is easy to filter after thorough layering, so it usually takes several hours to settle after the enzymatic hydrolysis, and the layering is to be performed. It is easy to carry out filtration and other operations after that, and the stratification can be completed within half an hour after adding the sedimentation agent; 2) The stratification can be made more thorough; It can completely replace the filtration process; and if no sedimentation agent is added, after a long time of standing, due to the light weight of pig brain residue, it will gradually aggregate and suspend in the liquid. The liquid interface is not smooth, and it is easy to become turbid by shaking and shaking a little, so the general preparation method has to choose the method of filtration or centrifugation; 3) The addition of sedimentation agent can change the texture of animal brain residue, from sticky to sticky. It is dispersed, easy to pour out, easy to handle, and can be used for harmless treatment such as composting; 4) After testing, the use of precipitation agent can improve the yield of cerebroprotein hydrolyzate, which may be because the precipitation agent reduces the concentration of animal brain residues. The viscosity makes the active ingredient easier to dissolve, that is, the precipitation agent reduces the viscosity of the animal brain residue, and it is not easy to wrap the active ingredient, thereby realizing the effect of improving the content of the active ingredient; 5) After the precipitation agent is processed, the supernatant obtained is clear and translucent , the subsequent processing procedures can be directly carried out, avoiding tedious and energy-consuming operations such as filtration and centrifugation.

The preparation method of the present invention further uses a flocculant to further treat the supernatant after treatment with a precipitation agent, so that macromolecular substances can be flocculated and precipitated out, the treatment speed is fast, and ultrafiltration and other treatments are not required. The flocculation treatment is combined with the sedimentation treatment in the previous step to further remove excess macromolecular substances, and achieve a similar effect to the traditional ultrafiltration process, but the time required is shorter and the operation is simpler. Compared with the filtration-ultrafiltration process, the sedimentation-flocculation process is used process, the total nitrogen content of the product is higher, that is, the effective ingredient retention of the substance is better.

The preparation method of the present invention does not need to perform an additional degreasing treatment step in the process of brain tissue processing. After the brain tissue is treated with a sedimentation agent, the oil layer (grease-like floating matter) on the surface can be skimmed off.

The preparation method of the present invention does not use an organic solvent, the process is simple and feasible, the energy consumption is low, and waste residue treatment is also considered, which is very environmentally friendly and conforms to the concept of green production.

Detailed ways

In order to facilitate understanding of the present invention, the present invention will be described more fully below, and preferred embodiments of the present invention will be given. However, the present invention may be embodied in many different forms and is not limited to the embodiments described herein. Rather, these embodiments are provided so that a thorough and complete understanding of the present disclosure is provided.

Unless otherwise defined, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terms used herein in the description of the present invention are for the purpose of describing specific embodiments only, and are not intended to limit the present invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

The preparation method of cerebroprotein hydrolysate according to an embodiment of the present invention includes the following steps S1-S5:

S1. The brain tissue is homogenized to obtain a homogenate.

S2. The homogenate is subjected to enzymolysis treatment to obtain an enzymolysis solution.

S3. Add a sedimentation agent to the enzymatic hydrolysis solution, and after standing for stratification, remove the oil layer on the surface, and collect the sedimentation supernatant.

S4, adding a flocculant to the sedimentation supernatant, centrifuging, and collecting the centrifuging supernatant.

S5. Add activated carbon to the centrifugal supernatant, keep the temperature at 50°C to 70°C for 10min to 30min, filter and collect the filtrate to obtain a cerebroprotein hydrolysate.

The preparation method of cerebroprotein hydrolysate of the present invention has the following advantages:

The preparation method of the present invention is treated by adding a sedimentation agent after enzymatic hydrolysis: 1) the sedimentation time can be shortened; because the animal brain residue is particularly sticky, it is easy to filter after thorough layering, so it usually takes several hours to settle after the enzymatic hydrolysis, and the layering is to be performed. It is easy to carry out filtration and other operations after that, and the stratification can be completed within half an hour after adding the sedimentation agent; 2) The stratification can be made more thorough; It can completely replace the filtration process; and if no sedimentation agent is added, after a long time of standing, due to the light weight of pig brain residue, it will gradually aggregate and suspend in the liquid. The liquid interface is not smooth, and it is easy to become turbid by shaking and shaking a little, so the general preparation method has to choose the method of filtration or centrifugation; 3) The addition of sedimentation agent can change the texture of animal brain residue, from sticky to sticky. It is dispersed, easy to pour out, easy to handle, and can be used for harmless treatment such as composting; 4) After testing, the use of precipitation agent can improve the yield of cerebroprotein hydrolyzate, which may be because the precipitation agent reduces the concentration of animal brain residues. The viscosity makes the active ingredient easier to dissolve, that is, the precipitation agent reduces the viscosity of the animal brain residue, and it is not easy to wrap the active ingredient, thereby realizing the effect of improving the content of the active ingredient; 5) After the precipitation agent is processed, the supernatant obtained is clear and translucent , the subsequent processing procedures can be directly carried out, avoiding tedious and energy-consuming operations such as filtration and centrifugation.

The preparation method of the present invention further uses a flocculant to further treat the supernatant after treatment with a precipitation agent, so that macromolecular substances can be flocculated and precipitated out, the treatment speed is fast, and ultrafiltration and other treatments are not required. The flocculation treatment is combined with the sedimentation treatment in the previous step to further remove excess macromolecular substances, and achieve a similar effect to the traditional ultrafiltration process, but the time required is shorter and the operation is simpler. Compared with the filtration-ultrafiltration process, the sedimentation-flocculation process is used process, the total nitrogen content of the product is higher, that is, the effective ingredient retention of the substance is better.

The preparation method of the present invention does not need to perform an additional degreasing treatment step in the process of brain tissue processing. After the brain tissue is treated with a sedimentation agent, the oil layer (grease-like floating matter) on the surface can be skimmed off.

The preparation method of the present invention does not use an organic solvent, the process is simple and feasible, the energy consumption is low, and waste residue treatment is also considered, which is very environmentally friendly and conforms to the concept of green production.

In a specific example, before adding the precipitation agent, the enzymatic hydrolysis solution is heated to boiling, and after the precipitation agent is added, the solution is continuously boiled for 10-20 min before standing for stratification. After heating and boiling, adding a precipitation agent and continuing to boil for a period of time can not only inactivate the enzymes added during enzymatic hydrolysis treatment, but also make the precipitation agent and brain residue better combine, reduce the viscosity of animal brain residue, and improve active ingredients. At the same time, the sedimentation agent can be better dispersed evenly in the boiling state, which is convenient for better combination with animal brain residues, and helps to achieve the purpose of rapid sedimentation. If the supernatant is too late for the next flocculation treatment immediately, the supernatant can be heated and boiled again for about 10min, and left overnight for the next day treatment.

In a specific example, the settling agent is selected from one or more of talc, diatomaceous earth and calcium carbonate. Preferably, the settling agent is diatomaceous earth.

In a specific example, the mass ratio of the homogenate to the sedimentation agent is 100:(0.5-4), and a better sedimentation effect can be obtained. Optionally, when the brain residue is separated and the supernatant is collected, the brain residue is washed with water once, which can improve the yield of the cerebroprotein hydrolysate.

In a specific example, the flocculant is selected from one or more of gelatin, chitosan, egg white powder and 101 juice clarifying agent. Preferably, the flocculant is chitosan. By comparing the effects of flocculants such as egg white (powder), gelatin, chitosan, and 101 fruit juice clarifying agent, it is found that chitosan has the best effect, which is mainly reflected in two aspects, one is the clarity of the solution after treatment, Whether there will be precipitation in the subsequent treatment, on the other hand, whether the loss of active ingredient content can be minimized.

In a specific example, the mass ratio of the sedimentation supernatant to the flocculant is 100:(0.06～0.09), which can obtain a better flocculation effect, and the mass ratio of the centrifugal supernatant to the activated carbon is 100:(1.5～5) .

In a specific example, the enzymatic hydrolysis treatment includes the following steps: mixing the homogenate with water and protease, and performing a hydrolysis reaction at 40°C to 45°C for 8 to 24 hours. In this way, the hydrolysis can be effectively carried out without adjusting the pH of the enzymatic hydrolysis. If the hydrolysis is carried out in an acidic or alkaline environment, multi-step treatments such as centrifugation and filtration are required after the hydrolysis, and the acid-base neutralization during the hydrolysis will generate a large amount of salt. Not only will it affect the taste of the product, but high salt is also very detrimental to human health, and it takes energy to remove salt (such as desalination by electrodialysis, etc.).

In a specific example, proteases include pepsin and trypsin. It can be understood that the types of enzymes are not limited thereto, such as papain, hydroxylamine, serine protease and the like.

In a specific example, the amount of pepsin added is 3% to 5% of the mass of the brain tissue, and the amount of trypsin added is 4% to 6% of the mass of the brain tissue, so that better enzymes can be obtained. solution effect.

In a specific example, the brain tissue is one or more of porcine brain tissue, sheep brain tissue, and bovine brain tissue. It can be understood that the type of brain tissue is not limited to this, and the brain tissue of various other animals can be used.

In a specific example, before the homogenization treatment, the following steps are further included: washing the brain tissue to remove blood water and impurities.

In a specific example, the preparation method further includes the following steps: spray-drying the cerebroprotein hydrolysate to prepare a cerebroprotein hydrolysate powder or directly prepare a liquid preparation.

The embodiments of the present invention will be described in detail below with reference to specific examples.

Example 1

Take 5kg of pig brain, clean it, homogenize it, and place it in a hydrolysis tank.

Add 5kg of purified water to the hydrolysis tank, turn on the heating and stirring functions, control the temperature at 40-45°C, add 200g of pepsin and 250g of trypsin, stir evenly, and keep hydrolysis for 8h.

Heat the enzymatic hydrolysis solution to boil, add 100 g of diatomaceous earth when it is slightly boiled, and continue to boil for 20 min. After sedimentation and stratification, the pig brain residue at the bottom of the tank was released, and the supernatant was collected for use.

Chitosan was added to the supernatant, and the mass ratio of the supernatant to the chitosan was 100:0.075, and the mixture was stirred while adding. Centrifuge and collect supernatant.

Activated carbon was added to the supernatant at a mass ratio of 100:2.5, heated with stirring at 60° C. for 20 min, and the filtrate was collected by filtration to obtain a cerebroprotein hydrolysate.

The obtained cerebroprotein hydrolyzate is spray-dried to prepare cerebroprotein hydrolyzate powder.

Example 2

Take 50kg of pig brain, clean it, homogenize it, and place it in a hydrolysis tank.

Add 50kg of purified water to the hydrolysis tank, turn on the heating and stirring functions, control the temperature at 40-45°C, add 2000 g of pepsin and 2500 g of trypsin, stir evenly, and keep hydrolysis for 8 hours.

Heat the enzymatic hydrolysis solution to boil, add 1000g of diatomaceous earth when it is slightly boiled, and continue to boil for 20min. After sedimentation and stratification, the pig brain residue at the bottom of the tank was released, and the supernatant was collected for use.

Chitosan was added to the supernatant, and the mass ratio of the supernatant to the chitosan was 100:0.075, and the mixture was stirred while adding. Centrifuge and collect supernatant.

Activated carbon was added to the supernatant at a mass ratio of 100:2.5, heated with stirring at 60° C. for 20 min, and the filtrate was collected by filtration to obtain a cerebroprotein hydrolysate.

The obtained cerebroprotein hydrolyzate was spray-dried to prepare cerebroprotein hydrolyzate powder.

Example 3

Take 5kg of pig brain, clean it, homogenize it, and place it in a hydrolysis tank.

Add 5kg of purified water to the hydrolysis tank, turn on the heating and stirring functions, control the temperature at 40-45°C, add 200g of pepsin and 250g of trypsin, stir evenly, and keep hydrolysis for 8h.

The enzymatic hydrolysis solution was heated to 80°C, 100 g of diatomaceous earth was added, and the temperature was kept at 80°C for 20 min. After sedimentation and stratification, the pig brain residue at the bottom of the tank was released, and the supernatant was collected for use.

Chitosan was added to the supernatant, and the mass ratio of the supernatant to the chitosan was 100:0.075, and the mixture was stirred while adding. Centrifuge and collect supernatant.

Activated carbon was added to the supernatant at a mass ratio of 100:2.5, heated with stirring at 60° C. for 20 min, and the filtrate was collected by filtration to obtain a cerebroprotein hydrolysate.

The obtained cerebroprotein hydrolyzate was spray-dried to prepare cerebroprotein hydrolyzate powder.

Example 4

Take 5kg of pig brain, clean it, homogenize it, and place it in a hydrolysis tank.

Add 5kg of purified water to the hydrolysis tank, turn on the heating and stirring functions, control the temperature at 40-45°C, add 200g of pepsin and 250g of trypsin, stir evenly, and keep hydrolysis for 8h.

The enzymatic hydrolysis solution was heated and boiled, 100 g of talc was added when it was slightly boiled, and the boil was continued for 20 min. After sedimentation and stratification, the pig brain residue at the bottom of the tank was released, and the supernatant was collected for use.

Chitosan was added to the supernatant, and the mass ratio of the supernatant to the chitosan was 100:0.075, and the mixture was stirred while adding. Centrifuge and collect supernatant.

Activated carbon was added to the supernatant at a mass ratio of 100:2.5, heated with stirring at 60° C. for 20 min, and the filtrate was collected by filtration to obtain a cerebroprotein hydrolysate.

The obtained cerebroprotein hydrolyzate was spray-dried to prepare cerebroprotein hydrolyzate powder.

Example 5

Take 5kg of pig brain, clean it, homogenize it, and place it in a hydrolysis tank.

Add 5kg of purified water to the hydrolysis tank, turn on the heating and stirring functions, control the temperature at 40-45°C, add 200g of pepsin and 250g of trypsin, stir evenly, and keep hydrolysis for 8h.

Heat the enzymatic hydrolysate to a boil, add 100 g of calcium carbonate when it is slightly boiled, and continue to boil for 20 minutes. After sedimentation and stratification, the pig brain residue at the bottom of the tank was released, and the supernatant was collected for use.

Chitosan was added to the supernatant, and the mass ratio of the supernatant to the chitosan was 100:0.075, and the mixture was stirred while adding. Centrifuge and collect supernatant.

Activated carbon was added to the supernatant at a mass ratio of 100:2.5, heated with stirring at 60° C. for 20 min, and the filtrate was collected by filtration to obtain a cerebroprotein hydrolysate.

The obtained cerebroprotein hydrolyzate was spray-dried to prepare cerebroprotein hydrolyzate powder.

Example 6

Take 5kg of pig brain, clean it, homogenize it, and place it in a hydrolysis tank.

Add 5kg of purified water to the hydrolysis tank, turn on the heating and stirring functions, control the temperature at 40-45°C, add 200g of pepsin and 250g of trypsin, stir evenly, and keep hydrolysis for 8h.

Heat the enzymatic hydrolysis solution to boil, add 100 g of diatomaceous earth when it is slightly boiled, and continue to boil for 20 min. After sedimentation and stratification, the pig brain residue at the bottom of the tank was released, and the supernatant was collected for use.

Add gelatin to the supernatant, and the mass ratio of the supernatant to gelatin is 100:0.075, and the mixture is added and stirred. Centrifuge and collect supernatant.

Activated carbon was added to the supernatant at a mass ratio of 100:2.5, heated with stirring at 60° C. for 20 min, and the filtrate was collected by filtration to obtain a cerebroprotein hydrolysate.

The obtained cerebroprotein hydrolyzate was spray-dried to prepare cerebroprotein hydrolyzate powder.

Example 7

Take 5kg of pig brain, clean it, homogenize it, and place it in a hydrolysis tank.

Add 5kg of purified water to the hydrolysis tank, turn on the heating and stirring functions, control the temperature at 40-45°C, add 200g of pepsin and 250g of trypsin, stir evenly, and keep hydrolysis for 8h.

Heat the enzymatic hydrolysis solution to boil, add 100 g of diatomaceous earth when it is slightly boiled, and continue to boil for 20 min. After sedimentation and stratification, the pig brain residue at the bottom of the tank was released, and the supernatant was collected for use.

The egg white powder is added to the supernatant, and the mass ratio of the supernatant to the egg white powder is 100:0.075, and the mixture is stirred and heated while adding. Centrifuge and collect supernatant.

Activated carbon was added to the supernatant at a mass ratio of 100:2.5, heated with stirring at 60° C. for 20 min, and the filtrate was collected by filtration to obtain a cerebroprotein hydrolysate.

The obtained cerebroprotein hydrolyzate was spray-dried to prepare cerebroprotein hydrolyzate powder.

Example 8

Take 5kg of pig brain, clean it, homogenize it, and place it in a hydrolysis tank.

Add 5kg of purified water to the hydrolysis tank, turn on the heating and stirring functions, control the temperature at 40-45°C, add 200g of pepsin and 250g of trypsin, stir evenly, and keep hydrolysis for 8h.

Heat the enzymatic hydrolysis solution to boil, add 100 g of diatomaceous earth when it is slightly boiled, and continue to boil for 20 min. After sedimentation and stratification, the pig brain residue at the bottom of the tank was released, and the supernatant was collected for use.

Add 101 fruit juice clarifying agent to the supernatant liquid, the mass ratio of the supernatant liquid and 101 fruit juice clarifying agent is 100:0.075, and stir while adding. Centrifuge and collect supernatant.

Activated carbon was added to the supernatant at a mass ratio of 100:2.5, heated with stirring at 60° C. for 20 min, and the filtrate was collected by filtration to obtain a cerebroprotein hydrolysate.

The obtained cerebroprotein hydrolyzate was spray-dried to prepare cerebroprotein hydrolyzate powder.

Comparative Example 1

Take 5kg of pig brain, clean it, homogenize it, and place it in a hydrolysis tank.

Add 5kg of purified water to the hydrolysis tank, turn on the heating and stirring functions, control the temperature at 40-45°C, add 200g of pepsin and 250g of trypsin, stir evenly, and keep hydrolysis for 8h.

Add 100 g of diatomaceous earth to the enzymatic hydrolysis solution to aid in filtration, stir for 30 min, filter, and collect the filtrate.

The filtrate is subjected to ultrafiltration to collect components with a molecular weight of less than 5000 Daltons, and then nanofiltration and concentration are carried out with a nanofiltration machine with a molecular weight cut-off of 90 to 150 Daltons to obtain a cerebroprotein hydrolysate.

The obtained cerebroprotein hydrolyzate was spray-dried to prepare cerebroprotein hydrolyzate powder.

Comparative Example 2

5kg pig brain was taken, washed, and homogenized to obtain a homogenate.

Add 3 times the volume of acetone to the homogenate, stir and stand still, filter and collect the filter residue, and dry to obtain brain protein powder.

Put the brain protein powder in a hydrolysis tank, add 5kg of purified water, turn on the heating and stirring functions, control the temperature at 40-45°C, add 200g of pepsin and 250g of trypsin, stir evenly, and keep hydrolysis for 8h.

The enzymatic hydrolysate was centrifuged for 20 min, and the supernatant was collected to obtain cerebroprotein hydrolysate.

The obtained cerebroprotein hydrolyzate was spray-dried to prepare cerebroprotein hydrolyzate powder.

Comparative Example 3

Take 5kg of pig brain, clean it, homogenize it, and place it in a hydrolysis tank.

Add 5kg of purified water to the hydrolysis tank, turn on the heating and stirring functions, control the temperature at 40-45°C, add 200g of pepsin and 250g of trypsin, stir evenly, and keep hydrolysis for 8h.

Heat the enzymatic hydrolysis solution to boil, add 100 g of diatomaceous earth when it is slightly boiled, and continue to boil for 20 min. After sedimentation and stratification, the pig brain residue at the bottom of the tank was released, and the supernatant was collected for use.

Activated carbon was added to the supernatant in a mass ratio of 100:2.5 without flocculant, and the mixture was heated at 60°C for 20 minutes with stirring. The filtrate was collected by filtration, centrifuged and spray-dried to prepare cerebroprotein hydrolysate powder.

Comparative Example 4

Take 5kg of pig brain, clean it, homogenize it, and place it in a hydrolysis tank.

Add 5kg of purified water to the hydrolysis tank, turn on the heating and stirring functions, control the temperature at 40-45°C, add 200g of pepsin and 250g of trypsin, stir evenly, and keep hydrolysis for 8h.

Chitosan was added to the enzymatic hydrolysis solution, the mass ratio of the enzymatic hydrolysis solution and chitosan was 100:7.5, and the mixture was stirred while adding. The layers were left to stand, the precipitate was released from the bottom, and the supernatant was collected.

100 g of diatomaceous earth was added to the supernatant, boiled for 20 minutes and then left to stand for stratification, the precipitate at the bottom of the tank was released, and the supernatant was collected.

Activated carbon was added to the supernatant at a mass ratio of 100:2.5, heated at 60° C. for 20 min with stirring, filtered to collect the filtrate, and spray-dried to prepare cerebroprotein hydrolysate powder.

The product quality of the cerebroprotein hydrolyzate powder prepared by each embodiment and the comparative example is tested, as follows:

The technical features of the above-described embodiments can be combined arbitrarily. For the sake of brevity, all possible combinations of the technical features in the above-described embodiments are not described. However, as long as there is no contradiction between the combinations of these technical features, All should be regarded as the scope described in this specification.

The above-mentioned embodiments only represent several embodiments of the present invention, and the descriptions thereof are specific and detailed, but should not be construed as a limitation on the scope of the invention patent. It should be pointed out that for those of ordinary skill in the art, without departing from the concept of the present invention, several modifications and improvements can also be made, which all belong to the protection scope of the present invention. Therefore, the protection scope of the patent of the present invention should be subject to the appended claims.

Claims (10)

1. a preparation method of cerebroprotein hydrolysate, is characterized in that, comprises the following steps: The brain tissue is homogenized to obtain a homogenate; The homogenate is subjected to enzymolysis treatment to obtain an enzymolysis solution; Add a sedimentation agent to the enzymatic hydrolyzate, and after standing for stratification, remove the oil layer on the surface, and collect the sedimentation supernatant; A flocculant is added to the sedimentation supernatant, centrifuged, and the centrifugal supernatant is collected; Activated carbon is added to the centrifugal supernatant, the temperature is kept at 50° C. to 70° C. for 10 min to 30 min, and the filtrate is collected after filtration to obtain the cerebroprotein hydrolysate. 2. preparation method according to claim 1, is characterized in that, before adding described settling agent, described enzymolysis solution is heated to boiling, and after adding described settling agent, continues to boil before standing stratification 10min～20min. 3. preparation method according to claim 1 is characterized in that, described settling agent is selected from one or more in talc, diatomaceous earth and calcium carbonate. 4. The preparation method according to claim 3, wherein the mass ratio of the homogenate to the sedimentation agent is 100:(0.5～4). 5. The preparation method according to claim 1, wherein the flocculant is selected from one or more of gelatin, chitosan, egg white powder and 101 fruit juice clarifying agent. 6. preparation method according to claim 5 is characterized in that, the mass ratio of described sedimentation supernatant and described flocculant is 100:(0.06～0.09), described centrifugal supernatant and described gac The mass ratio is 100:(1.5～5). 7 . The preparation method according to claim 1 , wherein the enzymatic hydrolysis treatment comprises the following steps: mixing the homogenate with water and protease, and performing a hydrolysis reaction at 40° C. to 45° C. for 8 . ~24 hours. 8. The preparation method according to claim 7, wherein the protease comprises pepsin, papain and trypsin. 9 . The preparation method according to claim 8 , wherein the amount of the pepsin added is 3% to 5% of the mass of the brain tissue, and the amount of the trypsin added is the mass of the brain tissue. 10 . 4% to 6%. 10 . The preparation method according to claim 1 , wherein the brain tissue is one or more of porcine brain tissue, sheep brain tissue and bovine brain tissue. 11 .