CN111549386A - Vegetable and berry fruit fermentation product rayon fiber mask for moisturizing and resisting aging - Google Patents

Abstract

The invention provides a vegetable berry fruit fermentation rayon fiber facial mask for moisturizing and aging resistance, which comprises a piece of rayon fiber cloth for increasing liquid carrying capacity and improving the stability of a cloth structure, and a vegetable berry fruit fermentation substance, and has the effects of increasing the water content and elasticity of skin, improving skin texture and smoothing wrinkles.

Description

A kind of fermented rayon fiber mask for moisturizing and anti-aging

technical field

The present invention relates to a facial mask, in particular to a rayon fiber facial mask used for moisturizing and anti-aging of vegetables and fruits (or vegetables and berries) fermented.

Background technique

At present, the largest amount of raw materials used in the production of non-woven fabrics made of man-made synthetic fibers are polypropylene (PP), polyester (PET), polyethylene (PE) and nylon (Nylon), which together account for the total use of When these large amounts of man-made synthetic fiber non-woven fabrics become waste after use, they cannot be decomposed by the natural environment and cause great harm to the environment. Therefore, wood pulp (pulp) is used as raw material and a solvent method is used. The production of natural cellulose non-woven fabrics has gradually become the mainstream, and its product waste is called green environmental protection fiber because it can be naturally degraded.

Rayon, also known as rayon, is made from natural plant cellulose, so it is a regenerated fiber. Rayon has the properties of easy dyeing, washing and acid and alkali resistance. It is often used in clothing and interior decoration (such as bedspreads and blankets). In industry, rayon is also commonly used to manufacture surgical instruments, non-woven products, feminine hygiene products, and tire cords. Wait.

However, the general rayon fabric will be loose due to swelling, and the water absorption, tensile strength and mechanical strength still need to be strengthened. In order to solve the above-mentioned problems, those skilled in the art are in urgent need of developing a novel method for preparing rayon cloth, and the tensile strength, mechanical strength, density and water absorption of the cloth, cloth film or mask thus obtained are all required. Upgrading to benefit the majority of ethnic groups in need.

SUMMARY OF THE INVENTION

In view of the problems faced by the prior art, an object of the present invention is to provide a rayon fiber yarn, which comprises a plurality of supporting fibers, the plurality of supporting fibers are obtained by alkalizing the fiber raw material, and the fiber raw material is obtained. Regenerated fibers made of plant cellulose; and nano-filled fibers, which are embedded between a plurality of supporting fibers.

In an embodiment of the present invention, the plant cellulose is a regenerated fiber made from cellulose raw materials such as wood pulp, wood chips or cotton chips, also known as rayon.

In an embodiment of the present invention, the alkalization treatment is performed with sodium hydroxide.

In an embodiment of the present invention, the nano-filled fibers are slender fibrous structures prepared by grinding bacterial cellulose, the average diameter of which is less than 50 nanometers. Preferably, bacterial cellulose is milled to prepare nano-filled fibers with a diameter of 1-30 nanometers and a length of about 1-20 micrometers (preferably the length is below 2 micrometers).

In an embodiment of the present invention, the diameter of the nano-filled fibers is 1-4 nanometers.

In an embodiment of the present invention, nano-filled fibers are emerging industrial materials, mainly used in mask materials, high-strength materials (automobile parts, home appliance shells), high-functional materials (residential building materials, interior materials), tackifiers ( food, medical supplies), and special materials (special paper, filter material).

Another object of the present invention is to provide a nano rayon fiber cloth, comprising: a plurality of the aforementioned rayon fiber filaments, which are interlaced with each other to form a piece of cotton web.

Another object of the present invention is to provide a facial mask for moisturizing and anti-aging, comprising: the aforementioned nano-rayon fiber cloth; and a fermented product adsorbed in the nano-rayon fiber cloth.

In an embodiment of the present invention, the fermented product is obtained by fermenting vegetable and berry juice, and the vegetable and berry juice is selected from blueberry juice, cranberry juice, white radish juice, carrot juice, bayberry juice, At least one of the group consisting of mulberry juice, grape juice, apple juice, sugar cane juice, passion fruit juice, pineapple juice, lemon juice, broccoli juice, celery juice and asparagus juice.

In an embodiment of the present invention, the fermentation reaction comprises: mixing vegetable juice and water in a weight ratio of 1:1-5, sterilizing at 50-100° C. for 0.5-1.5 hours; then cooling it to room temperature; Saccharomyces cerevisiae BCRC20271 was sequentially implanted in the cooled vegetable juice, the yeast accounted for 0.01-0.5wt% of the whole vegetable juice, and then fermented for 1-3 days; lactic acid bacteria (Streptococcus thermophilus TCI633) were implanted in the previous step The obtained vegetable juice, the lactic acid bacteria accounts for 0.01-0.25wt% of the whole vegetable juice at this time, and then fermented for 1-5 days; then concentrated under reduced pressure at 45-70 DEG C; finally, sieve with 200-400 mesh (mesh) The fermented product can be obtained by filtration.

Another object of the present invention is to provide a method for preparing nano-rayon fiber cloth, comprising the following steps: (a) alkalizing the fiber raw material to obtain a plurality of supporting fibers; (b) mixing nano-filled fibers with A plurality of supporting fibers are thoroughly mixed to obtain a fiber slurry mixture, wherein the nano-filled fibers comprise bacterial cellulose, and the diameter of the nano-filled fibers is 1-30 nanometers; (c) the fiber slurry mixture is formed into a plurality of rayon fibers by a forming step silk, wherein the nano-filled fibers are embedded between the supporting fibers; and (d) through a spinning step, a plurality of rayon fiber filaments are formed into a nano-rayon fiber cloth, so as to increase the liquid carrying capacity and improve the stability of the cloth structure .

In an embodiment of the present invention, in step (b), the ratio of the weight of the nano-filled fibers to the weight of the supporting fibers is 1:10 to 1:20.

In an embodiment of the present invention, in step (a), the fiber raw material comprises one or a combination of wood pulp, wood chips, or cotton chips.

In an embodiment of the present invention, in step (a), the alkalization treatment is performed with sodium hydroxide.

In an embodiment of the present invention, the diameter of the nano-filled fibers is 1-4 nanometers.

Another object of the present invention is to provide a nano rayon fiber cloth, which is obtained by the method as described above.

In an embodiment of the present invention, the stability of the cloth structure of the nano rayon fiber cloth is that the tensile strength is greater than 20N.

In an embodiment of the present invention, the stability of the cloth structure of the nano-rayon fiber cloth is that the elongation ratio is greater than 120%.

Another object of the present invention is to provide a facial mask made of the aforementioned nano-rayon fiber cloth, the liquid carrying capacity of which is greater than 10 grams of water.

To sum up, the effect of the nano rayon fiber cloth of the present invention is that the skin feels much better than that of man-made fibers, it is suitable for the development of improved mask materials, it is easier to make the nano-filled fibers evenly distributed in liquid state, and it can improve the original The tensile strength, ductility, mechanical strength, density and water absorption of rayon cloth are an improved cloth film material. Therefore, the mask made of the nano-rayon fiber cloth can absorb more essences containing fermented fruits and vegetables, so that the mask can be more compliant when applied to the skin, and has better importability, thereby enabling The antioxidant and skin beautifying functions of the fermented vegetables and berries are more effectively introduced into the skin, resulting in a multiplier effect.

The embodiments of the present invention will be further described below. The following examples are used to illustrate the present invention, but not to limit the scope of the present invention. Anyone skilled in the art, without departing from the spirit and scope of the present invention, While some changes and modifications may be made, the scope of protection of the present invention should be determined by the scope defined by the appended claims.

Description of drawings

Fig. 1 is the structural representation of nano rayon fiber cloth in one embodiment of the present invention;

Fig. 2 is the density comparison diagram of nanometer rayon fiber cloth in one embodiment of the present invention;

Fig. 3 is the electron microscope structure observation diagram of nano rayon fiber cloth in one embodiment of the present invention;

Fig. 4 is the function data diagram of nano rayon fiber cloth in improving the mechanical strength of rayon cloth in an embodiment of the present invention;

5 is a graph of the efficacy data of nano rayon fiber cloth in improving the elongation rate of rayon cloth in an embodiment of the present invention;

Fig. 6 is the efficacy data diagram of nano rayon fiber cloth in improving the water absorption of rayon cloth in an embodiment of the present invention;

7 is a comparison diagram of the oxidative radical absorption capacity (ORAC) of vegetable and berry fermented product and vegetable and berry juice in an embodiment of the present invention;

FIG. 8 is a graph showing the comparison of the activity of superoxide dismutase (superoxide dismutase, SOD) of fruit and vegetable fermented product and fruit and vegetable juice in an embodiment of the present invention;

FIG. 9 is a comparison diagram of the collagen production and synthesis gene of skin fibroblasts by fermented vegetables and berries in an embodiment of the present invention;

Figure 10 is a comparison diagram of the elastin production and synthesis gene of skin fibroblasts by fermented vegetables and berries in an embodiment of the present invention;

Fig. 11 is the result diagram of skin water content after using vegetable berry fermented product for 15 minutes in an embodiment of the present invention;

12 is a graph showing the results of a skin elasticity test after 15 minutes of using the fermented fruit and vegetables in an embodiment of the present invention;

FIG. 13 is a graph showing the results of skin texture test after using the fermented fruit and vegetables for 15 minutes in an embodiment of the present invention.

FIG. 14 is a graph showing the results of skin wrinkle test after using the fermented fruit and vegetables for 15 minutes in an embodiment of the present invention.

Among them, reference numerals:

Support Fiber 11

Nano-Filled Fibers 12

rayon filament 13

Nano rayon fiber cloth 14

Detailed ways

Various embodiments of the present invention will be disclosed in the drawings below, and for the sake of clarity, many practical details will be described in the following description. However, these practical details should not be used to limit the invention. That is, in some embodiments of the invention, these practical details are unnecessary. Numerical values used herein are approximations and all experimental data are expressed within 20%, preferably within 10%, and most preferably within 5%.

As used herein, unless the context specifically defines the article, "a" and "the" can refer to a single one or a plurality. It can be further understood that the words "comprising", "including", "having" and similar words used herein indicate the features, regions, integers, steps, operations, components and/or components described therein, but do not exclude one or more other features, regions, integers, steps, operations, components, components, and/or groups thereof, described or additional thereto.

From the foregoing, it can be known that the general rayon cloth will be loose due to swelling, and the water absorption, tensile strength and mechanical strength still need to be strengthened. In view of this, the present invention provides a nano-rayon fiber cloth, which has good tensile strength, mechanical strength, density and water absorption. It can be further combined with the "fruit and vegetable fermented essence containing vegetable and berry (hereinafter referred to as "vegetable and berry fermented essence"), which has good moisturizing and anti-aging functions provided by the present invention, and then becomes a vegetable and berry fermented product. Essence's nano-rayon fiber mask. This mask can not only absorb more fermented fruit and vegetable essence with moisturizing and anti-aging functions, but also use the good tensile strength, mechanical strength and density of the mask to better introduce the fermented fruit and vegetable essence into the skin , so that the skin has better moisturizing and anti-aging ability.

The preparation method and efficacy test of the nano-rayon fiber cloth provided by the present invention and the nano-rayon fiber mask of vegetable, berry and fruit fermented essence will be described in detail below to confirm that the mask has good absorption, tensile strength, The mechanical strength and density are improved, so that the cells can more easily absorb the essence; and it is further confirmed that the vegetable, berry and fruit fermented essence provided by the present invention has the functions of moisturizing and anti-aging.

Example 1. Preparation of supporting fibers, preparation of nano-filled fibers and test of fabric structure stability

Please refer to FIG. 1 , which is a schematic structural diagram of the nano-rayon fiber cloth 14 . First, the supporting fibers 11 are prepared first, wherein the fiber raw materials of the supporting fibers 11 are regenerated fibers made from cellulose raw materials such as wood pulp, wood chips, or cotton chips, also known as rayon. The fiber raw material is alkalized with sodium hydroxide to obtain alkalized and homogeneous supporting fibers 11, which may also be referred to as supporting rayon fibers in the present invention. Next, nano-filling fibers 12 are prepared, wherein the nano-filling fibers 12 are selected from bacterial cellulose, and are prepared into nano-filling fibers 12 with a diameter of about 1-30 nanometers and a length of about 1-20 microns by grinding, and the preferred length is 2 below microns.

The nano-filled fibers 12 are added to the alkali-treated supporting fibers 11 and mixed thoroughly to obtain a fiber slurry mixture, wherein the weight ratio of the nano-filled fibers 12 to the supporting fibers 11 is 1:10 to 1:20. After that, the liquid fiber slurry mixture is subjected to a molding step: the fiber slurry mixture is sprayed out through a spinneret, and coagulated into filaments in an aqueous sulfuric acid solution to form rayon fiber filaments 13 .

Please refer to FIG. 1 , the supporting fibers 11 are long fibers without being ground. Therefore, after being ejected from the spinneret, a plurality of long supporting fibers 11 form a single rayon fiber filament, wherein there are gaps between the long supporting fibers 11 , and the nano-filled fibers 12 will fit into the gaps between the plurality of long supporting fibers 11 , that is, at least part of the nano-filled fibers 12 are located in a single rayon fiber, so that a denser rayon fiber is formed. Next, after the weaving step, the rayon fiber filaments 13 can be further loosened into smaller cotton bundles and cotton clumps by, for example, a cotton opener, and then evenly placed in, for example, a carding machine to form a cotton web. After that, stack the cotton web to a desired thickness of about 0.01-0.5 cm, and then put it into a needle-tie machine, such as a needle-bonding machine, to form a cloth, so as to form a nano-rayon fiber cloth 14 .

The manufacturing process of the above nano-rayon fiber cloth 14 can be commissioned by FORMOSACHEMICALS & FIBRE CORPORATION.

In another embodiment of the present invention, the nano-filled fibers have a diameter in the range of 1-30 nanometers, and a length of about 1-20 micrometers, and a preferred length is less than 2 micrometers.

In another embodiment of the present invention, the nanofilled fibers have a diameter in the range of 1-4 nanometers.

In the embodiment of the present invention, the filling fiber is preferably made of bacterial cellulose, which is also called biological fiber.

Next, the fabric structure stability (density) test was performed on the nano rayon fiber cloth 14, and compared with the general rayon cloth film (without filler fibers). The manufacturing method of the "general rayon cloth film" (or "general rayon cloth") described in the present invention is roughly the same as that of the nano-rayon fiber cloth 14, and the difference is only that the nano-filling fibers 12 are not added. The comparison results are shown in FIG. 2 . Figure 2 is a comparison chart of the density of nano rayon fiber cloth. As can be seen from Figure 2, compared with the general rayon cloth film, the nanometer rayon fiber cloth can maintain the original structure in water, while the general rayon cloth film will become loose due to swelling. The results of this example show that the appearance of the nano rayon fiber cloth is denser than that of the general rayon cloth film, and it can maintain a stable and compact rayon fiber silk structure even when placed in a liquid. Therefore, the nano rayon fiber cloth of the present invention can effectively Improve the density of rayon cloth.

In an embodiment of the present invention, when the ratio of nano-filled fibers to support fibers is increased, the overall smooth touch of the cloth body is also increased. For example, when the weight ratio of nano-filled fibers: supporting fibers in the cloth body or in the slurry before forming the cloth body is adjusted from 1:20 to 1:10, it can be found that when the weight ratio of nano-filled fibers: supporting fibers is 1: 10 o'clock, compared with 1:20, it has better tactile feeling.

Example 2. Electron microscope structure observation of nano rayon fiber cloth

Conductive SilverPaint,30g#16062)粘贴于金属载台。接着，将载台放入Hitachi Tabletop Microscope TM-1000桌上型电子显微镜内部，于真空环境下观测不同倍率下纤维的外观型态。图3是纳米嫘萦纤维布的电子显微镜结构观察图。由图3可见，以6000倍电子显微镜观察纳米嫘萦纤维布膜的结构型态，可发现纳米填充纤维嵌于嫘萦的纤维束中(如箭头所示)，一般嫘萦布膜则无。In an embodiment of the present invention, the general rayon cloth film and the nano-rayon fiber cloth in Example 1 were cut into test sheets with a length of 1 cm and a width of 1 cm, respectively, and carbon glue (purchased from TED PELLA,

Conductive SilverPaint, 30g#16062) is attached to the metal stage. Next, the stage was put into the Hitachi Tabletop Microscope TM-1000 desktop electron microscope, and the appearance of the fibers at different magnifications was observed in a vacuum environment. Fig. 3 is the electron microscope structure observation diagram of the nano-rayon fiber cloth. It can be seen from Figure 3 that the structure of the nano-rayon fiber cloth film was observed with a 6000x electron microscope, and it can be found that the nano-filled fibers are embedded in the fiber bundles of rayon (as shown by the arrow), and the general rayon cloth film is not.

Example 3. Mechanical strength and material ductility test of nano rayon fiber cloth

In this example, the mechanical properties of the nano-rayon fiber cloth and the general rayon cloth in Example 1 were tested, including mechanical strength (ie, tensile strength) and elongation ( elongation). First, the film-forming nano-rayon fiber cloth and the general rayon cloth are stacked in three layers, and are respectively cut into tensile test pieces with a length of 5 cm and a width of 1 cm, and the thickness of each tensile test piece is measured. Next, the tensile strength of the film was tested with a Shimadzu EZ Tester 500N strength testing machine. The distance between the upper and lower clips was 1.5 cm, and the test was carried out at a tensile rate of 10 mm/min. Both sides were clamped on the fixture. After that, the stress-strain curve and the maximum load value of the stretching process were recorded, and the maximum stress of the nano-rayon fiber cloth and the general rayon cloth under the same basis weight was compared. When the fiber is stretched to complete breakage, its maximum extensible length is recorded, and the tensile strength and elongation rate can be obtained from the stress-strain diagram to compare the physical strength of each material. The results of this example are shown in FIGS. 4 and 5 .

Figure 4 is a data graph of the efficacy of nano-rayon fiber cloth in enhancing the mechanical strength of rayon cloth. It can be seen from Figure 4 that compared with the general rayon cloth, the maximum stress of the nano-rayon fiber cloth is increased by 90%, which confirms that the nano-rayon fiber cloth has higher mechanical strength. Figure 5 is a data graph of the efficacy of nano-rayon fiber cloth in increasing the elongation of rayon cloth. As can be seen from Figure 5, compared with the general rayon cloth, the elongation rate of the nano-rayon fiber cloth is increased by 20%, which proves that the nano-rayon fiber cloth has excellent ductility.

Example 4. Material liquid loading capacity test of nano rayon fiber cloth

First, the general rayon cloth film and the nano rayon fiber cloth in Example 1 were cut into test sheets with a length of 10 cm and a width of 10 cm, and the weights were recorded, and then the two test sheets were immersed in 50 ml of pure water 30 cm minute. The results of this example are shown in FIG. 6 . Figure 6 is a graph of the efficacy data of nano rayon fiber cloth in improving the water absorption of rayon cloth. As can be seen from Figure 6, since the rayon fiber filaments embedded with nano-filled fibers can be filled with nano-filled fibers, the water absorption of the nano-rayon fiber cloth formed by the rayon fiber filaments embedded with nano-filled fibers is: 1.9 times that of general rayon cloth. The results of this example show that the nano-rayon fiber cloth can effectively improve the water absorption of the rayon cloth.

In an embodiment of the present invention, when the ratio of nano-filled fibers to support fibers is increased, the hygroscopic force is also increased. For example, when the weight ratio of nano-filled fibers: supporting fibers in the cloth body or in the slurry before forming the cloth body is adjusted from 1:20 to 1:10, its hygroscopic force will also increase. This is because the nano-rayon fiber cloth is embedded with nano-filled fibers, so the overall surface area is increased, so it has better hygroscopicity than the general rayon cloth.

To sum up, the nano-rayon fiber cloth of the present invention has a much better skin feel than general rayon cloth and other man-made fibers, is suitable for developing improved mask materials, and is easier to make the nano-filled fibers evenly distributed in liquid state, and can be It can effectively improve the tensile strength, ductility, mechanical strength, density and water absorption of the original rayon cloth, which is an improved cloth film material.

Embodiment 5. Vegetable and berry fermented product preparation process in rayon fiber mask

According to an embodiment of the present invention, the steps of the preparation process of the vegetable, berry and fruit fermented product are as follows:

1. Mix the comprehensive berry juice (MX-AC130-FC, purchased from Yang Ya Marketing Co., Ltd.) and the comprehensive vegetable berry juice (MX-AC134-FL, purchased from Yang Ya Marketing Co., Ltd.) at a weight ratio of 1:1 Then obtain a vegetable and berry juice (or can be called vegetable juice), then mix the vegetable and berry juice with water in a weight ratio of 1:1-5, and sterilize at 50-100 ° C for 0.5-1.5 hours;

2. The vegetable and berry juice obtained in step 1 is cooled to room temperature (25±5°C) for subsequent fermentation;

3. Implant yeast (Saccharomyces cerevisiae BCRC20271) in the vegetable and berry juice obtained in step 2 (after implantation, the yeast accounts for 0.01-0.5wt% of the whole vegetable and berry juice), and ferment for 1-3 days;

4. Implant lactic acid bacteria (Streptococcus thermophilusTCI633) in the vegetable and berry juice obtained in step 3 (after implantation, lactic acid bacteria account for 0.01-0.25wt% of the whole vegetable and berry juice), and ferment for 1-5 days;

5. Concentrate under reduced pressure at 45-70°C;

6. Filter with 200-400 mesh (mesh) mesh screen to obtain a vegetable and berry fermented product, and the total polyphenol content of the vegetable and berry fermented product is more than 40 micrograms/ml (μg/mL), and its SOD is Above 700U/mL.

In some embodiments, the vegetable and berry juice (or can be called vegetable juice) used in the aforementioned step 1 can be selected from blueberry juice, cranberry juice, white radish juice, carrot juice, bayberry juice, mulberry juice, At least one selected from the group consisting of grape juice, apple juice, sugar cane juice, passion fruit juice, pineapple juice, lemon juice, broccoli juice, celery juice, and asparagus juice.

Example 6. Analysis of Oxidative Radical Absorption Capacity (ORAC) of Vegetable and Berry Fermented Products and Vegetable and Berry Juice

In an embodiment of the present invention, two experimental samples of equal weight are prepared, one of which is the "vegetable and berry juice" obtained in step 1 of Example 5, and the other is obtained from steps 1-6 in Example 5. The "vegetable and berry fermented product" obtained by fermentation; the aforementioned "vegetable and berry fruit juice" and "vegetable and berry fermented product" were sent to Tendums Technical Consultants Co., Ltd. for ORAC analysis. The results are shown in Figure 7. As can be found in Figure 7, the fermented vegetable and berry fruit provided by the present invention has an oxidative radical absorption capacity of 1.4 times that of vegetable berry fruit juice. It can be seen that after the vegetable berry fruit juice is fermented into vegetable berry fruit juice fermented product, it can improve the original Oxidative radical absorption capacity.

Example 7. Analysis of SOD activity of vegetable and berry fermented product and vegetable and berry juice

An embodiment of the present invention is a test experiment of the activity of superoxide dismutase (SOD) in the fermented vegetables, berries and fruits of the present invention, which refers to the determination of the activity of superoxide dismutase in GB/T5009.171-2003 health food. Criteria for testing, and also compare the fruit and vegetable juice described in Example 6 with the fruit and vegetable fermented product, in which SOD Quantification of activity. SOD is an enzyme that can catalyze the disproportionation reaction of superoxide and convert it into oxygen and hydrogen peroxide. It exists widely in various animals, plants, and microorganisms. It is an important antioxidant in living organisms. Protects cells exposed to oxygen.

First, the A solution and the B solution for the measurement were prepared, wherein the A solution was a mixture of 1.2114 g of Tris(hydroxymethyl)aminomethane (Tris) and 37.2 mg (mg) of ethylenediaminetetraacetic acid (EDTA). -2Na) was dissolved in 62.4 milliliters (mL) of hydrochloric acid (HCl) solution with a concentration of 0.1 mole/L, and quantified to 100 milliliters with pure water after dissolving, and solution B was 56.7 mg of pyrogallol (pyrogallol) ) was dissolved in a small amount of hydrochloric acid with a concentration of 10 mmole/L and after being dissolved, quantified to 100 ml with pure water.

Treat the sample to be tested, wherein the solid sample is prepared by the following method: weigh 1 gram of the solid sample and place it in a mortar, add 9 ml of pure water to grind and dissolve it, transfer the whole liquid into a centrifuge tube, and rinse the grind with a small amount of pure water. After mixing, the remaining liquid was put into a centrifuge tube and quantified with pure water to a total volume of 10 ml. After centrifugation at 4000 rpm for 15 minutes, the supernatant was taken for measurement. Liquid samples are prepared in the following ways: clear liquid samples can be measured directly in the stock solution; turbid liquid samples are centrifuged at 4000 rpm for 5 minutes, and the supernatant is taken for measurement. Liquid samples can be appropriately diluted with pure water, and finally multiplied by the dilution factor to calculate the original concentration.

Next, please refer to the following table 1 to carry out the blank measurement of the control group: add 2.35 ml of A solution and 2 ml of pure water to the centrifuge tube in sequence, after shaking and mixing evenly, add 0.15 ml of B solution, add B solution and mix Then, shake and mix evenly, and immediately pour it into a colorimetric tube to measure its absorbance at 325 nm wavelength. After 1 minute of reaction, measure its absorbance at 325 nm wavelength again. The difference between the two values The value is ΔA ₃₂₅ ; and the measurement of the sample to be tested is to add 2.35 ml of A solution, 1.8 ml of pure water, and 0.2 ml of the sample to be tested in the centrifuge tube. fruit juice, vegetable, berry and fruit ferment) and 0.15 ml of B solution, after adding B solution and mixing, shake quickly and gently to mix evenly, and immediately pour it into a colorimetric tube to measure its absorbance at 325 nm wavelength, react for 1 minute The absorbance value at the wavelength of 325 nm is detected again, and the difference between the two values is ΔA' ₃₂₅ .

Table 1. Scale for SOD activity determination

reagent blank sample A solution 2.35ml 2.35ml pure water 2 ml 1.8ml Sample to be tested - 0.2ml B solution 0.15ml 0.15ml

Finally, the SOD activity in the fruit and vegetable juice and the fermented fruit and vegetables was calculated by the following formula, where V was 4.5 and D was 0.2.

ΔA ₃₂₅ : pyrogallol auto-oxidation rate

ΔA′ ₃₂₅ : The sample inhibits the auto-oxidation rate of pyrogallol

V: the volume of the sample

D: the dilution factor of the sample

The test results of the efficacy of the superoxide dismutase activity in the fermented vegetable and berry fruit of the present invention are shown in Figure 8. From Figure 8, it can be found that the superoxide dismutase activity in the vegetable berry fruit fermented product is 1.7 times that of the vegetable berry fruit juice. The results show that the fermented vegetable, berry and fruit of the present invention can enhance the activity of superoxide dismutase, so as to achieve the purpose of enhancing the health-care antioxidant effect.

Example 8. Analysis of collagen and elastin biosynthesis genes in skin fibroblasts of fermented fruits and vegetables

The present invention uses human skin fibroblasts (CCD-966sk) to conduct cell experiments on the fermented fruit and vegetables of the present invention. The human dermal fibroblasts were purchased from the American Type Culture Collection, number CRL-1881. The cells were cultured in MEM (Minimum essential medium, purchased from Gibco, USA, 12100-046) containing 10% Fetal Bovine Serum, in which 1 mM sodium pyruvate and 1 % Penicillin/Streptomycin.

In this example, the aforementioned human dermal fibroblasts were cultured in a 24-well culture dish containing the aforementioned MEM medium, 2×10 ⁴ cells were implanted in each well, and the cells were cultured at 37° C. for 24 hours. The cells are then divided into two groups: the first group is a control group (Control); the second group is an experimental group, and in the experimental group, the vegetable, berry and fruit fermented product of Example 5 is added, and its final concentration of the vegetable, berry and fruit fermented product is 1 mg/mL. Next, the following steps were performed after culturing at 37°C for 24 hours.

III Reverse Transcriptase(Invitrogen)将2000ng RNA反转录为cDNA。其后，通过目标基因与作为内部对照的甘油醛3-磷酸脱氢酶(Glyceraldehyde 3-phosphate dehydrogenase，GAPDH)基因的引子对(表二)，利用qPCR套组(KAPA CYBR FAST qPCR Kit(2X)；KAPA Biosystems)在PCR反应仪(Step One PlusReal-Time PCR system；Applied Biosystems)对前述cDNA进行qPCR，以取得解链曲线(melting curve)。Based on quantitative polymerase chain reaction (quantitative polymerase chain reaction, referred to as qPCR), the expression of genes (target genes) related to collagen and elastin biosynthesis in cells is determined. The steps are briefly described as follows. According to the manufacturer's instructions, use RNA Extraction Kit (Geneaid) to isolate RNA from cells, and at 37°C with reverse transcriptase

III Reverse Transcriptase (Invitrogen) reverse transcribed 2000 ng of RNA to cDNA. Then, through the primer pair of the target gene and the Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) gene as an internal control (Table 2), using the qPCR kit (KAPA CYBR FAST qPCR Kit (2X) ; KAPA Biosystems) in a PCR reactor (Step One Plus Real-Time PCR system; Applied Biosystems) to perform qPCR on the aforementioned cDNA to obtain a melting curve.

Table 2. Primer sequence list

Finally, the relative expression amount of the target gene was determined using the 2 ^-ΔΔCT method. In this method, the cycle threshold ( _CT ) of the GAPDH gene is used as the cycle threshold of the reference gene of the internal control, and the relative fold change is calculated according to the following formula:

ΔC _T = _CT of target gene in experimental or control group - _CT of internal control

ΔΔCT = ΔC _T of the experimental group - ΔC _T of the control group

Fold change = 2 ^{- ΔΔCT mean}

Statistical analysis is to use the STDEV function in Excel software to calculate the standard deviation of the relative expression of each gene, and to calculate the statistical difference by one-tailed Student's t test (TTEST).

In this example, the genes COL1A1 and α2-type I collagen (collagen, type I, alpha 1) were detected in the control group and the experimental group (fermented vegetables, berries and fruits). alpha 2) gene COL1A2 and elastin (Elastin) gene ELN expression level, to prove whether the vegetable berry fermented product provided by the present invention has the function of promoting the synthesis of collagen and elastin, please refer to Figure 9, 10. From Figure 9, it can be found that the expression levels of COL1A1 and COL1A2 genes are 1.22 and 1.26 times that of the control group, respectively (* means p<0.05). It can also be seen from Figure 10 that the expression level of ELN is 1.29 times that of the control group (* ** means p<0.001), it can be seen that the vegetable, berry and fruit fermented product provided by the present invention can indeed increase the expression of collagen and elastin genes, thereby improving the synthesis of collagen and elastin.

Embodiment 9. Human body efficacy test of vegetable and berry fermented mask

The experimental samples of this embodiment are vegetable and berry fermented facial masks and placebo facial masks, wherein the cloths used in the vegetable berry fermented facial mask and the placebo facial mask are the same, and the vegetable berry fermented facial mask and the placebo facial mask contain For liquid substances, the composition ratios are shown in Table 3:

Table 3. Composition ratio of liquid substances contained in the mask

Fruit and vegetable fermented mask (%) Placebo Mask (%) Fruit and vegetable fermented product of Example 5 80 - Antioxidants 0.6 0.6 moisturizer 5 5 thickener 0.3 0.3 Solubilizers 0.6 0.6 pH balancer 0.1 0.1 water 13.4 93.4

Wherein, for example, the antioxidant can be Hydroxyacetophenone; the humectant can be 1,3-butanediol; the thickener can be Xanthan gum or/and acrylate/C10-30 Alkyl acrylate cross-linked polymer (Acrylates/C10-30 Alkyl Acrylate Crosspolymer), solubilizer can be 1,2-Hexanediol (1,2-Hexanediol); pH balance agent can be Triethanolamine (Triethanolamine).

In the experiment of this embodiment, there are 8 subjects, and each of them applied the aforementioned vegetable, berry and fruit fermented mask and placebo mask to the left and right half of the face respectively, removed it after 15 minutes, and massaged with finger pulp to promote absorption. , and use the VISIA Complexion Analysis System (purchased from Canfield scientific, USA) to test before and after use. The test items are: skin moisture content, elasticity, texture state and wrinkle state. "Texture state detection" is to use the instrument to calculate the smoothness of the skin based on the height difference of the cheeks, and "wrinkle state detection" is to use the instrument to evaluate the depth and quantity of wrinkles around the eyes. The results are shown in Figure 11-14 shown.

It can be seen from FIG. 11 that after the subject uses the vegetable, berry and fruit fermented mask of the present invention, the skin water content can be significantly increased by 13.7% compared with before use (** means p<0.01), while the placebo mask only It can be increased by 3.2%, which is not significant; it can be seen from Figure 12 that the skin elasticity of the subjects after using the vegetable, berry and fruit fermented mask of the present invention is increased by 4.4% compared with before use, while the placebo mask only There is a 0.9% change; it can be seen from Figure 13 that the skin texture state of the subjects after using the vegetable berry fermented mask of the present invention is improved by 2.9% compared with before use, while after using the placebo mask, the skin texture state is improved by 2.9%. There is no significant difference from before use; finally, it can be seen from Figure 14 that after the subject uses the fermented vegetable, berry and fruit facial mask of the present invention, the skin wrinkle state is greatly improved by 50% compared with before use (* means p< 0.05), compared with a 29.4% improvement with the placebo mask.

It can be seen from the above that the fermented vegetable, berry and fruit facial mask of the present invention can effectively increase the water content and elasticity of the skin, and has the skin-beautifying effects of improving texture and smoothing wrinkles.

It can be seen from the above-mentioned embodiments or examples of the present invention that the nano-rayon fiber cloth provided by the present invention is an improved mask material, which has a much better skin feel than man-made fibers, and can improve the tensile strength and elongation of the original rayon cloth. capacity, mechanical strength, density and water absorption; in addition, the fermented vegetables and berries provided by the present invention can improve the absorption capacity of cellular oxidative free radicals and the anti-oxidative effect, and can also improve the production and synthesis of collagen and elastin. The efficacy test further proved that the fermented vegetable and berry mask can effectively increase the moisture content and elasticity of the skin, and has the effect of improving texture and smoothing wrinkles. The essence can more effectively introduce the antioxidant and skin-beautifying functions of the fermented vegetables and berries into the skin, resulting in a multiplier effect.

The above description is exemplary only, not limiting. Any equivalent modifications or changes without departing from the spirit and scope of the present invention should be included in the scope defined by the appended claims.

sequence listing

<110> Dajiang Biomedical Co., Ltd.

<120> A fermented rayon fiber mask for moisturizing and anti-aging

<150> US62/804,228, US 62/871,216

<151> 2019-02-12, 2019-07-08

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Claims (15)

1. A rayon filament characterized by comprising:

a plurality of supporting fibers obtained by alkalizing a fiber raw material, which is regenerated fibers made of plant cellulose; and

and the nano filling fibers are embedded among the plurality of supporting fibers.

2. The rayon fiber filament according to claim 1, characterized in that said plant cellulose is derived from wood pulp, wood chips or cotton chips.

3. The rayon fiber filament according to claim 1, characterized in that said alkalizing treatment is carried out with sodium hydroxide.

4. The rayon filament according to claim 1, characterized in that said nano-filler fiber is made of bacterial cellulose by grinding.

5. The rayon fiber filament according to claim 1 or 4, characterized in that said nano-filler fiber has a diameter of 1 to 30 nanometers.

6. The rayon fiber filament according to claim 1 or 4, characterized in that said nano-filler fiber has a diameter of 1 to 4 nanometers.

7. A nano rayon fiber cloth characterized by comprising:

a plurality of rayon fiber filaments according to any one of claims 1 to 6, interlaced with one another into a web.

8. A facial mask for moisturizing and anti-aging, comprising:

the nano rayon fiber cloth according to claim 7; and

and the ferment adsorbed in the nano rayon fiber cloth.

9. The mask of claim 8, wherein the fermented product is obtained by fermentation of a vegetable juice, and the vegetable juice is at least one selected from the group consisting of blueberry juice, cranberry juice, white radish juice, red bayberry juice, mulberry juice, grape juice, apple juice, sugarcane juice, passion fruit juice, pineapple juice, lemon juice, broccoli juice, celery juice, and asparagus juice.

10. The mask of claim 9, wherein the fermentation reaction comprises:

mixing the vegetable juice with water at a weight ratio of 1:1-5 to obtain a mixed solution of the vegetable juice and the water, and sterilizing and extracting at 50-100 ℃ for 0.5-1.5 hours;

cooling to room temperature;

implanting yeast (Saccharomyces cerevisiae BCRC20271) in the cooled mixed solution of the vegetable and fruit juice and water, wherein the yeast accounts for 0.01-0.5 wt% of the whole mixed solution of the vegetable and fruit juice and then fermenting for 1-3 days;

implanting lactobacillus (Streptococcus thermophilus TCI633) in the mixed solution of the vegetable and fruit juice and water obtained in the previous step, wherein the lactobacillus accounts for 0.01-0.25 wt% of the whole mixed solution of the vegetable and fruit juice and water, and fermenting for 1-5 days;

concentrating under reduced pressure at 45-70 deg.C; and

filtering with 200-400 mesh sieve to obtain the fermented product.

11. A method for preparing a nano rayon fiber cloth, characterized by comprising the steps of:

(a) performing alkalization treatment on a fiber raw material to obtain a plurality of supporting fibers, wherein the fiber raw material is regenerated fibers made of plant cellulose;

(b) fully mixing nano filling fibers with the plurality of supporting fibers to obtain a fiber slurry mixture, wherein the nano filling fibers comprise bacterial cellulose, and the diameter of the nano filling fibers is 1-30 nanometers;

(c) forming the fiber slurry mixture into a plurality of rayon fibers by a forming step, wherein the nano-filler fibers are embedded between the plurality of supporting fibers; and

(d) the plurality of rayon filaments are formed into the nano rayon fiber cloth through the weaving step.

12. The method of claim 11, wherein in step (b), the ratio of the weight of the nano-filler fibers to the weight of the plurality of support fibers is from 1:10 to 1: 20.

13. The method of claim 11, wherein in step (a), the fibrous feedstock comprises one or a combination of wood pulp, wood chips, or cotton chips.

14. The method according to claim 11, wherein in step (a), the alkalization treatment is performed with sodium hydroxide.

15. The method of claim 11, wherein the diameter of the nano-filler fiber is 1-4 nanometers.

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