CN111548919A - Magnetic control type tissue crushing bacteria extraction device and extraction method - Google Patents

Abstract

The invention discloses a magnetically controlled tissue smashing bacteria extraction device and an extraction method, belonging to the field of medical testing equipment, comprising a container, wherein a magnetic rotary drive device is arranged at the bottom of the container; A rotating impeller; the magnetic rotation driving device is composed of a rotating motor connected with a rotating base; the rotating base is provided with a magnet for attracting the impeller. Its main advantages are that it can significantly improve the discovery rate of acid-fast bacilli, and the product is low in cost, easy to use, and can prevent cross-contamination of test results.

Description

Magnetic control type tissue crushing bacteria extraction device and extraction method

technical field

The invention belongs to the field of medical testing equipment, in particular to a magnetic control type tissue crushing bacteria extraction device and an extraction method.

Background technique

Pathological tissue biopsy is a very important and frequently used diagnostic method in the medical field. Since it is used in a medical environment, in order to avoid cross-infection, all current pathological detection products must use disposable products, generally after tissue biopsy. The way. However, the pathological diagnosis of acid-fast bacilli in tissue sections is very difficult, because the bacteria are embedded in the tissue. Even after ultrathin sectioning, the distribution of bacteria is scattered and difficult to find. Therefore, the detection results of acid-fast bacilli in the tissue are inaccurate. It is often necessary to eliminate the error rate by increasing the detection amount.

SUMMARY OF THE INVENTION

In view of the above problems, the present invention provides a magnetically controlled tissue crushing bacteria extraction device and an extraction method. Its main advantages are that the discovery rate of acid-fast bacilli can be significantly improved, the product cost is low, the use is convenient, and the detection test results can be prevented. Pollution.

For achieving the above purpose, the technical scheme adopted in the present invention is:

A magnetic control type tissue crushing bacteria extraction device, comprising a container, a magnetic rotary drive device is arranged at the bottom of the container; an impeller driven and rotated by the magnetic rotary drive device is placed inside the container; the magnetic force rotary drive device is driven by a rotary motor It is formed by connecting with a rotating seat; the rotating seat is provided with a magnet for sucking the impeller.

As a further improvement of the present invention, the rotation axis of the rotating seat is perpendicular to the horizontal plane; the upper end surface of the rotating seat is arranged in parallel with the bottom surface of the container.

As a further improvement of the present invention, two magnets are arranged on the rotating seat, which are respectively located at two ends of the top surface of the rotating seat.

As a further improvement of the present invention, the magnetic rotary drive device is arranged below the table of the rack; the container is placed on the table.

As a further improvement of the present invention, the impeller is an iron component; the center bottom of the impeller is provided with a downwardly protruding rotating fulcrum.

As a further improvement of the present invention, the impeller is provided with at least one pair of main blades and at least one pair of auxiliary blades; the length of the main blades is longer than that of the auxiliary blades.

As a further improvement of the present invention, upper inclined surfaces are provided on both sides of the upper end surface of the main blade; and lower inclined surfaces are provided on the bottom surface of the auxiliary blade.

As a further improvement of the present invention, a filter screen is provided at the outlet of the container.

As a further improvement of the present invention, the mesh size of the filter screen is 100-120 meshes.

A magnetic control type tissue crushing bacteria extraction method, which is composed of the following operation steps:

Step 1. Put the tissue block to be inspected and the tissue lysate into the container;

Step 2. Place the container on the table, just above the rotating seat;

Step 3. Start the rotating motor, the rotating seat rotates through the magnetic attraction to drive the impeller to rotate at a high speed, and the impeller smashes the tissue into a paste;

Step 4: Pour out the liquid containing acid-fast bacilli in the container, filter out tissue fragments, and obtain a liquid specimen containing acid-fast bacilli.

Beneficial effects of the present invention:

1. The present invention uses magnetic force to control the high-speed rotation of the impeller, so that the device can not only isolate bacteria and viruses, prevent cross-infection, but also make the rotation of the impeller cut the tissue, which can pulverize the tissue into a paste, and promote the anti-acid in the tissue. Bacillus into the lysate, thereby improving the accuracy of the detection results of acid-resistant bacilli.

2. In the present invention, the impeller is freely placed in the container, and the resistance received is small. At the same time, auxiliary blades are arranged, and the length of the auxiliary blades is shorter than that of the main blade, which can play a balancing role, so that the impeller is more stable during the rotation process, and the rotating speed is at the same time. faster.

3. In the present invention, upper slopes are arranged on both sides of the upper end surface of the main blade, and lower slopes are arranged on the lower end surface of the auxiliary vanes, which cooperate with each other to further reduce the friction force of the impeller during high-speed rotation, form a floating rotation, and greatly improve the rotation speed of the impeller, Improve cutting effect.

4. The device of the present invention can not only realize the function of cutting tissue, but also realize the effect of rotation and oscillation because the blade can reach a very high speed, which further promotes the discharge of bacteria in the tissue and improves the extraction effect.

Description of drawings

Fig. 1 is the overall structure schematic diagram of the present invention;

Fig. 2 is the top-view structure schematic diagram of the rotating seat in the present invention;

Figure 3 is a side view of the impeller;

Figure 4 is a top view of the impeller;

5 is a structural diagram of an impeller in another embodiment;

In the figure: 1. Container; 2. Cover; 3. Drainage port; 4. Filter screen; 5. Impeller; 6. Rotary seat; 7. Magnet; 8. Table top; 9. Rotary motor; 52, the main blade; 53, the auxiliary blade; 521, the upper slope; 531, the lower slope.

Detailed ways

In order to make those skilled in the art better understand the technical solutions of the present invention, the present invention will be described in detail below with reference to the accompanying drawings. The description in this part is only exemplary and explanatory, and should not have any limiting effect on the protection scope of the present invention. .

As shown in FIG. 1-FIG. 5, a specific embodiment of the present invention has a structure: a magnetic control type tissue crushing bacteria extraction device, including a container 1, and a magnetic rotation drive device is provided at the bottom of the container 1; the interior of the container 1 is An impeller 5 driven to rotate by a magnetic rotation driving device is placed; the magnetic rotation driving device is composed of a rotating motor 9 connected with a rotating base 6 ; the rotating base 6 is provided with a magnet 7 for attracting the impeller 5 . The magnetic rotary drive device can be fixedly arranged under the bottom surface of the container 1; it can also be separated from the container 1 into an independent part; the container 1 can be set as a cup body structure, generally in the shape of a cylinder, and the upper port is provided with a sealing cover. , the container 1 should be in a sealed state. After the operation is completed, the outlet is opened, and the liquid is filtered and collected for testing. In the present invention, since the impeller and the magnetic rotary drive device are connected at a distance, the cleaning of the container is more convenient after the operation is completed, and the risk of cross infection can be reduced.

In a preferred embodiment, as shown in FIG. 1 , the rotation axis of the rotating base 6 is perpendicular to the horizontal plane; the upper end surface of the rotating base 6 is arranged in parallel with the bottom surface of the container 1 . The rotating seat 6 is located below the bottom surface of the container 1 , the magnet on the rotating seat 6 attracts the impeller, and when the rotating seat 6 rotates, the impeller rotates synchronously.

In a preferred embodiment, as shown in FIG. 2 , the rotating seat 6 is provided with two magnets 7 , which are respectively located at both ends of the top surface of the rotating seat 6 , and each magnet attracts both ends of the impeller respectively.

In a preferred embodiment, as shown in FIG. 1 , the magnetic rotary drive device is arranged below the table top 8 of the rack; the container 1 is placed on the table top 8 .

In a preferred embodiment, in order to enable the impeller 5 to be attracted by the magnet, the impeller 5 is an iron member; the center bottom of the impeller 5 is provided with a downwardly protruding rotating fulcrum 51 . The impeller 5 is placed freely at the bottom of the container, and is supported and rotated by the rotating fulcrum 51. Since the impeller is not rotated and fixed, the resistance to its rotation is very small. In order to improve the rotational balance of the impeller, the rotating fulcrum 51 is preferably a hemispherical type.

In a preferred embodiment, as shown in FIG. 4 , the impeller 5 is provided with at least one pair of main blades 52 and at least one pair of auxiliary blades 53 ; the length of the main blades 52 is longer than that of the auxiliary blades 53 . The main blade 52 is mainly used to realize rotary oscillation and rotary cutting, and the auxiliary blade is used to balance the rotation of the impeller, so that the impeller can form a more stable rotation with the help of the solution.

In a preferred embodiment, as shown in FIG. 5 , upper inclined surfaces 521 are provided on both sides of the upper end surface of the main blade 52 ; and lower inclined surfaces 531 are provided on the bottom surface of the auxiliary blade 53 . The two upper slopes 521 on the main blade 52, the upper slope on one side can promote the lysate to flow up and down while rotating, so that the tissue in the lysis solution can be cut by the main blade, and the upper slope on the other side When the impeller rotates at a high speed, the lysate can flow down along the upper slope, thereby entering the bottom of the auxiliary blade, and at the same time, the lower slope 531 at the bottom of the auxiliary blade generates a lifting force, and the lifting force generated by the auxiliary blade is still the same as that of the main impeller. The descending effect cancels each other out, making the impeller rotating at high speed with less resistance, increasing the impeller rotation speed, and also improving the impeller cutting effect.

In a preferred embodiment, in order to filter out the tissue in the lysate, a filter screen 4 is provided at the outlet of the container 1 .

In a preferred embodiment, in order to ensure the passage of acid-fast bacilli, the mesh size of the filter screen 4 to block most of the tissue fragments is 100-120 meshes.

A magnetic control type tissue crushing bacteria extraction method, which is composed of the following operation steps:

Step 1. Put the tissue block to be inspected and the tissue lysis solution into the container 1;

Step 2, placing the container 1 on the table top 8, just above the rotating seat 6;

Step 3. Start the rotating motor 9, the rotating base 6 rotates and drives the impeller 5 to rotate at a high speed through the magnetic attraction, and the impeller 5 pulverizes the tissue into a paste;

Step 4: Pour out the liquid containing the acid-fast bacilli in the container 1, filter out the tissue fragments, and obtain a liquid sample containing the acid-fast bacilli.

The core of the present invention is: a rotatable iron blade, a tissue block to be tested, and a tissue lysing solution are placed in a closed bacterial container. The high-speed rotation is driven by a strong magnetic device. During the rotation of the blade, the tissue is crushed into a paste, and the tissue lysate can quickly fluff the paste tissue. With the stirring effect produced by the rotation of the blade, the acid-fast bacilli are easily removed from the tissue. separated into solution. The lid of the container is designed with a cup mouth with a filter screen. When the liquid containing acid-fast bacilli is poured out, the tissue fragments are blocked on the filter screen to obtain a liquid sample containing acid-fast bacilli for further testing.

1. The blade is installed on the elongated iron member. The iron member and the blade can be driven to rotate by a strong magnet. The two sides of the central part of the iron member are raised to form a rotation fulcrum with the bottom of the cup. Strong magnets are mounted on the motor that can adjust the speed.

2. There are various formulations of tissue lysis solution, such as 20% sodium hypochlorite solution or trypsin.

3. The mesh size of the cup mouth filter is 100-120 mesh to ensure the passage of acid-fast bacilli and block most of the tissue fragments.

It should be noted that, herein, the terms "comprising", "comprising" or any other variation thereof are intended to encompass non-exclusive inclusion, such that a process, method, article or device comprising a series of elements includes not only those elements, It also includes other elements not expressly listed or inherent to such a process, method, article or apparatus.

Specific examples are used herein to illustrate the principles and implementations of the present invention, and the descriptions of the above examples are only used to help understand the method and the core idea of the present invention. The above are only the preferred embodiments of the present invention. It should be pointed out that due to the limited expression of words, there are objectively unlimited specific structures. For those of ordinary skill in the art, without departing from the principles of the present invention However, some improvements, modifications or changes can also be made, and the above-mentioned technical features can also be combined in an appropriate manner; these improvements, modifications, or combinations, or the concept and technical solutions of the invention are directly applied to other occasions without improvement. should be regarded as the protection scope of the present invention.

Claims (10)

1. A magnetic control type tissue crushing bacteria extraction device comprises a container (1), and is characterized in that a magnetic force rotation driving device is arranged at the bottom of the container (1); an impeller (5) driven to rotate by a magnetic rotation driving device is arranged in the container (1); the magnetic rotation driving device is formed by connecting a rotating motor (9) and a rotating seat (6); and a magnet (7) for attracting the impeller (5) is arranged on the rotating seat (6).

2. The magnetically controlled tissue pulverizing bacteria extracting apparatus as claimed in claim 1, wherein the rotation axis of the rotating base (6) is perpendicular to the horizontal plane; the upper end surface of the rotating seat (6) is parallel to the bottom surface of the container (1).

3. The magnetic-control tissue-pulverizing bacteria extraction device as claimed in claim 2, wherein the rotary seat (6) is provided with two magnets (7) respectively located at two ends of the top surface of the rotary seat (6).

4. The magnetically controlled tissue pulverizing bacteria extraction device of claim 1, wherein the magnetic rotary drive is disposed below the table (8) of the frame; the container (1) is placed on a table top (8).

5. A magnetically controlled tissue-disrupting bacteria extraction device as claimed in claim 1, in which the impeller (5) is a ferrous member; and a rotating fulcrum (51) protruding downwards is arranged at the bottom of the center of the impeller (5).

6. The magnetic-control tissue-pulverizing bacteria extraction device as claimed in claim 5, characterized in that the impeller (5) is provided with at least one pair of main blades (52) and at least one pair of auxiliary blades (53); the length of the main blade (52) is larger than that of the auxiliary blade (53).

7. The magnetic-control tissue-pulverizing bacteria extraction device as claimed in claim 6, wherein both sides of the upper end surface of the main blade (52) are provided with upper inclined surfaces (521); the bottom surface of the auxiliary blade (53) is provided with a lower inclined surface (531).

8. The magnetic-controlled tissue-pulverizing bacteria extraction device according to claim 1, characterized in that a filter screen (4) is provided at the outlet of the container (1).

9. The device for extracting bacteria by magnetic tissue disruption as claimed in claim 8, wherein the mesh size of said filter screen (4) is 100-120 meshes.

10. The method for extracting bacteria through magnetic control type tissue crushing according to the claims 1 to 9, characterized by comprising the following operation steps:

step one, placing a tissue block to be detected and a tissue lysate into the container (1);

secondly, the container (1) is placed on the table top (8) and is positioned right above the rotating seat (6);

step three, starting a rotating motor (9), driving an impeller (5) to rotate at a high speed by rotating a rotating seat (6) through magnetic attraction, and crushing the tissues into paste by the impeller (5);

and step four, pouring out the liquid containing the acid-fast bacillus in the container (1), and filtering out tissue fragments to obtain a liquid specimen containing the acid-fast bacillus.

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