CN1115335A - Method for prodn. of gallic acid by fermentation process - Google Patents
Method for prodn. of gallic acid by fermentation process Download PDFInfo
- Publication number
- CN1115335A CN1115335A CN 94107730 CN94107730A CN1115335A CN 1115335 A CN1115335 A CN 1115335A CN 94107730 CN94107730 CN 94107730 CN 94107730 A CN94107730 A CN 94107730A CN 1115335 A CN1115335 A CN 1115335A
- Authority
- CN
- China
- Prior art keywords
- gallic acid
- raw material
- hours
- tannin
- fermentative production
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- LNTHITQWFMADLM-UHFFFAOYSA-N gallic acid Chemical compound OC(=O)C1=CC(O)=C(O)C(O)=C1 LNTHITQWFMADLM-UHFFFAOYSA-N 0.000 title claims abstract description 30
- 235000004515 gallic acid Nutrition 0.000 title claims abstract description 15
- 229940074391 gallic acid Drugs 0.000 title claims abstract description 15
- 238000000034 method Methods 0.000 title claims description 14
- 238000000855 fermentation Methods 0.000 title abstract description 5
- 230000004151 fermentation Effects 0.000 title abstract description 5
- 239000002994 raw material Substances 0.000 claims abstract description 16
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- TUSDEZXZIZRFGC-UHFFFAOYSA-N 1-O-galloyl-3,6-(R)-HHDP-beta-D-glucose Natural products OC1C(O2)COC(=O)C3=CC(O)=C(O)C(O)=C3C3=C(O)C(O)=C(O)C=C3C(=O)OC1C(O)C2OC(=O)C1=CC(O)=C(O)C(O)=C1 TUSDEZXZIZRFGC-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000001263 FEMA 3042 Substances 0.000 claims abstract description 4
- LRBQNJMCXXYXIU-PPKXGCFTSA-N Penta-digallate-beta-D-glucose Natural products OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-PPKXGCFTSA-N 0.000 claims abstract description 4
- 239000008234 soft water Substances 0.000 claims abstract description 4
- 235000015523 tannic acid Nutrition 0.000 claims abstract description 4
- LRBQNJMCXXYXIU-NRMVVENXSA-N tannic acid Chemical compound OC1=C(O)C(O)=CC(C(=O)OC=2C(=C(O)C=C(C=2)C(=O)OC[C@@H]2[C@H]([C@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)[C@@H](OC(=O)C=3C=C(OC(=O)C=4C=C(O)C(O)=C(O)C=4)C(O)=C(O)C=3)O2)OC(=O)C=2C=C(OC(=O)C=3C=C(O)C(O)=C(O)C=3)C(O)=C(O)C=2)O)=C1 LRBQNJMCXXYXIU-NRMVVENXSA-N 0.000 claims abstract description 4
- 229940033123 tannic acid Drugs 0.000 claims abstract description 4
- 229920002258 tannic acid Polymers 0.000 claims abstract description 4
- 238000005406 washing Methods 0.000 claims abstract description 4
- 229920001864 tannin Polymers 0.000 claims description 10
- 235000018553 tannin Nutrition 0.000 claims description 10
- 239000001648 tannin Substances 0.000 claims description 10
- 230000007062 hydrolysis Effects 0.000 claims description 8
- 238000006460 hydrolysis reaction Methods 0.000 claims description 8
- 244000005700 microbiome Species 0.000 claims description 8
- 108010038851 tannase Proteins 0.000 claims description 7
- 238000012262 fermentative production Methods 0.000 claims description 6
- 238000002425 crystallisation Methods 0.000 claims description 5
- 230000008025 crystallization Effects 0.000 claims description 5
- 239000000463 material Substances 0.000 claims description 3
- 239000002893 slag Substances 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims description 3
- 239000012141 concentrate Substances 0.000 claims description 2
- 238000001035 drying Methods 0.000 claims description 2
- 238000001914 filtration Methods 0.000 claims description 2
- 238000011084 recovery Methods 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 238000003912 environmental pollution Methods 0.000 abstract description 3
- 230000001939 inductive effect Effects 0.000 abstract description 2
- 238000005516 engineering process Methods 0.000 description 4
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- 239000003513 alkali Substances 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 230000009466 transformation Effects 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 238000012258 culturing Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 239000002351 wastewater Substances 0.000 description 1
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The fermentation process for production of gallic acid includes immersing microbes after slant culture in solution of tannic acid for inducing for a while, immersing and washing spores with sterile soft water, breaking raw material and controlling its granularity, immersing the raw material in water and fermentation, and features, no environmental pollution and high recovery rate.
Description
The present invention relates to a kind of method of fermentative Production gallic acid.
At present, containing hydrolysis tannin plant material with Turkey-galls, Ta La or other produces the method for gallic acid and mainly contains hydrolysis method and microbe fermentation method, the shortcoming of hydrolysis method is that the transformation efficiency and the rate of recovery are all low, and the waste water that its production is produced also can produce certain environmental pollution; Microbe fermentation method of the prior art, as number of patent application is 93107994.2, name is called the application for a patent for invention of the technology of preparation of gallic acid by using enzyme, its disclosed processing method, though its transformation efficiency and the rate of recovery are all than hydrolysis method height, but owing to require to add alkali control pH value in its technology, so just increased the complicacy of production cost and technology, in addition in the prior art for enlarging when producing tannase inducing with seed with the microorganism behind the slant culture, owing to do not note adopting at a certain temperature pure tannase solution soaking microorganism in its technology, therefore generate other enzyme easily, do not note controlling raw material granularity in addition in the prior art, if raw material granularity too slightly is unfavorable for the leaching of tannin molecule, if raw material granularity too detailed rules and regulations can make tannin molecular diffusion condition destroyed and produce the powder conglomeration again, cause fluid flow resistance to strengthen, insolubles is increased, and these shortcomings of the prior art have all reduced the output of tannase and have reduced the rate of recovery of gallic acid.
The purpose of this invention is to provide and a kind ofly need not add alkali, rationally soak microorganism and control raw material granularity method with the fermentative Production gallic acid that improves gallic acid output.
The object of the present invention is achieved like this: it comprises that the microorganism behind the employing slant culture makes tannase, make Turkey-galls, Ta La or other contain tannin hydrolysis in the hydrolysis tannin plant material with tannase, with the tannin that is hydrolyzed after filtration, concentrate, crystallization, decolouring, drying and make gallic acid, microorganism behind the slant culture is soaked through 2%～8% pure tannic acid solution, under 28 ℃～34 ℃ temperature, induced the spore after soaking with aseptic soft water washing again 2～5 times 12 hours～24 hours; With raw material crushing, its granularity is controlled at 2～8mm, sterilizes then, also soaks raw material 8 hours～12 hours with the water yield that accounts for 7～10 times of raw material weights under 60 ℃～85 ℃ temperature; With 80 ℃～95 ℃ hot water washery slags and wash-out look gac, with the frozen water wash crystallization product below 4 ℃, to reduce the loss in the aftertreatment.
Compared with prior art, production cost of the present invention is low, and is free from environmental pollution, the rate of recovery height of raw material.
Accompanying drawing 1 is a process flow sheet of the present invention.
Go on foot the explanation embodiments of the invention below in conjunction with accompanying drawing nearly:
The proportioning of slant medium is as follows:
Distilled water 1000ml
NaNO
3 3g
K
2HPO
4 1g
MgSO
47H
2O 0.5g
Kd 0.5g
TeSO
4?7H
2O 0.01g
Sucrose 30g
Agar 15g
Pressurize 15 pounds sterilization in 30 minutes.
On the inclined-plane after the sterilization, carry out microbial inoculant.
Microorganism behind the slant culture is soaked through 2%～8% pure tannic acid solution, under 28 ℃～34 ℃ temperature, induced 12 hours～24 hours, again with the spore after the aseptic soft water washing immersion 2～5 times; Put it into afterwards and carry out the seed enlarged culturing in the following prescription:
NaNO
3 2.5g
K
2HPO
4 0.18g
μgSO
4?7H
2O 0.25g
Sucrose 15g
Tannin 50g
Yeast 0.5g
Water 1000ml
With raw material crushing, its granularity is controlled at about 5mm, uses the water yield of 7～10 times of raw material weights then, under 60 ℃～85 ℃ temperature, soak raw material 8 little right～12 hours; With 80 ℃～95 ℃ hot water washery slags and wash-out look gac, with the frozen water wash crystallization product below 4 ℃, to reduce the gallic acid loss in the aftertreatment.
Claims (3)
1, a kind of method of fermentative Production gallic acid, it comprises that the microorganism behind the employing slant culture makes tannase, make Turkey-galls, Ta La or other contain tannin hydrolysis in the hydrolysis tannin plant material with tannase, with the tannin that is hydrolyzed after filtration, concentrate, crystallization, decolouring, drying and make gallic acid, it is characterized in that: the microorganism behind the slant culture is soaked through 2%～8% pure tannic acid solution, under 28 ℃～34 ℃ temperature, induced the spore after soaking with aseptic soft water washing again 2～5 times 12 hours～24 hours.
2, the method for fermentative Production gallic acid according to claim 1, it is characterized in that: with raw material crushing, its granularity is controlled at 2～8mm, sterilizes then, also soaks raw material 8 hours～12 hours with the water yield that accounts for 7～10 times of raw material weights under 60 ℃～85 ℃ temperature.
3, the method for fermentative Production gallic acid according to claim 1 is characterized in that: with 80 ℃～95 ℃ hot water washery slags and wash-out look gac; With 4 ° of frozen water wash crystallization products below the C.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 94107730 CN1115335A (en) | 1994-07-16 | 1994-07-16 | Method for prodn. of gallic acid by fermentation process |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 94107730 CN1115335A (en) | 1994-07-16 | 1994-07-16 | Method for prodn. of gallic acid by fermentation process |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1115335A true CN1115335A (en) | 1996-01-24 |
Family
ID=5033191
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 94107730 Pending CN1115335A (en) | 1994-07-16 | 1994-07-16 | Method for prodn. of gallic acid by fermentation process |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1115335A (en) |
Cited By (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100436589C (en) * | 2001-06-22 | 2008-11-26 | 印度技术研究院 | A method for preparing gallic acid by co-cultivation |
| CN101280279B (en) * | 2008-04-09 | 2010-06-02 | 东北林业大学 | A Phomopsis strain capable of producing gallic acid |
| CN101864459A (en) * | 2010-05-07 | 2010-10-20 | 遵义市倍缘化工有限责任公司 | Method for preparing gallic acid |
| CN102443614A (en) * | 2011-11-10 | 2012-05-09 | 湖南农业大学 | Method for separating and purifying epigallocatechin (EGC) monomer |
| CN102643871A (en) * | 2012-04-17 | 2012-08-22 | 昆明怡普康植物化学有限公司 | Preparation method of high-purity gallic acid |
| CN103589758A (en) * | 2013-11-12 | 2014-02-19 | 遵义市倍缘化工有限责任公司 | Method for preparing gallic acid by using gallnut tannin as raw material and utilizing fermentation separation coupling technology |
| CN103893392A (en) * | 2012-12-25 | 2014-07-02 | 杭州桐君堂生物科技有限公司 | Method for improving gallic acid content of Chinese gall leaven |
| CN114250253A (en) * | 2020-09-22 | 2022-03-29 | 湖南利农五倍子产业发展有限公司 | Preparation process for processing propyl gallate from gallnut |
| WO2022268194A1 (en) * | 2021-06-24 | 2022-12-29 | 南京百斯杰生物工程有限公司 | Application of tannase in preparation of gallic acid |
-
1994
- 1994-07-16 CN CN 94107730 patent/CN1115335A/en active Pending
Cited By (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN100436589C (en) * | 2001-06-22 | 2008-11-26 | 印度技术研究院 | A method for preparing gallic acid by co-cultivation |
| CN101280279B (en) * | 2008-04-09 | 2010-06-02 | 东北林业大学 | A Phomopsis strain capable of producing gallic acid |
| CN101864459A (en) * | 2010-05-07 | 2010-10-20 | 遵义市倍缘化工有限责任公司 | Method for preparing gallic acid |
| CN102443614A (en) * | 2011-11-10 | 2012-05-09 | 湖南农业大学 | Method for separating and purifying epigallocatechin (EGC) monomer |
| CN102643871A (en) * | 2012-04-17 | 2012-08-22 | 昆明怡普康植物化学有限公司 | Preparation method of high-purity gallic acid |
| CN102643871B (en) * | 2012-04-17 | 2014-02-26 | 昆明怡普康植物化学有限公司 | Preparation method of high-purity gallic acid |
| CN103893392A (en) * | 2012-12-25 | 2014-07-02 | 杭州桐君堂生物科技有限公司 | Method for improving gallic acid content of Chinese gall leaven |
| CN103589758A (en) * | 2013-11-12 | 2014-02-19 | 遵义市倍缘化工有限责任公司 | Method for preparing gallic acid by using gallnut tannin as raw material and utilizing fermentation separation coupling technology |
| CN103589758B (en) * | 2013-11-12 | 2015-07-22 | 遵义市倍缘化工有限责任公司 | Method for preparing gallic acid by using gallnut tannin as raw material and utilizing fermentation separation coupling technology |
| CN114250253A (en) * | 2020-09-22 | 2022-03-29 | 湖南利农五倍子产业发展有限公司 | Preparation process for processing propyl gallate from gallnut |
| WO2022268194A1 (en) * | 2021-06-24 | 2022-12-29 | 南京百斯杰生物工程有限公司 | Application of tannase in preparation of gallic acid |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |