CN111466397A - Composition for preventing and treating silvery spodoptera and application thereof - Google Patents

Abstract

The invention discloses a composition for controlling Spodoptera frugiperda and its application. The composition of the present invention includes Spodoptera frugiperda sex pheromone and sex pheromone analogs, and the sex pheromone components are cis-7-dodecene acetate and cis-9-tetradecene acetate and cis-11-hexadecene-1-alcohol, the sex pheromone analogs are cis-7-dodecene-N-methyl carbamate, cis-7-dodecene-N, One or more of N-dimethyl carbamate and cis-7-dodecene-N-methyl-N-ethyl carbamate. The mass ratio of the sex pheromone of Spodoptera frugiperda to sex pheromone analogues is 1:0.01-0.1. The composition of the invention can more effectively trap and kill the male Spodoptera frugiperda, reduce the mating frequency and reduce the reproduction rate, thereby achieving the purpose of efficiently preventing and controlling Spodoptera frugiperda. The invention is not only low in dosage, but also environmentally friendly, and can also be accurately applied to insect situation forecasting, trapping and interfering with mating, and is of great significance for comprehensive control of Spodoptera frugiperda.

Description

A kind of composition for preventing and controlling Spodoptera frugiperda and application thereof

technical field

The invention belongs to the technical field of biological control, in particular to a composition for preventing and controlling Spodoptera frugiperda and application thereof.

Background technique

Macdunnoughia crassisigna Warren is a leaf-eating pest belonging to the order Lepidoptera and Noctuidae. In my country, it poses a serious threat to cotton, peanuts, corn, soybeans and cruciferous vegetables, and is one of the most destructive agricultural pests in my country. Spodoptera frugiperda occurs in 2-4 generations in my country, and its larvae harm different hosts in different periods. It mainly damages crops such as cotton and beans in spring, corn and soybeans in summer, and cruciferous vegetables in autumn. The worm occurs in the cruciferous vegetables, and the common pests such as diamondback moth and cabbage worm occur at the same time. The newly hatched larvae gather on the back of the leaf to eat the mesophyll, leaving the upper epidermis, affecting the growth of the plant, and excreting feces to contaminate the vegetable plant. , which has a great impact on yield and quality. The female Spodoptera frugiperda has strong fecundity, and the eggs are scattered on the back of the leaves. Each female moth can lay 200 to 400 eggs in her lifetime. At present, the control of Spodoptera frugiperda mainly relies on chemical pesticides. However, due to the huge pollution of chemical pesticides to humans and the environment, excessive use can easily make Spodoptera frugiperda resistant. New prevention methods and new technologies are urgent needs to solve the current environmental pollution and food safety crisis.

The insect sex pheromone, known as the third-generation green pesticide, has attracted the attention of scientific researchers because of its high efficiency, non-toxicity, harmless to natural enemies, high sensitivity, and environmental protection. Insect sex pheromone is usually produced by mature female or male. Microchemicals secreted by insects and released into the body to induce mating in insects of the opposite sex. At present, more than 2,000 insect sex pheromones and their analogs have been identified and synthesized in the world, which are mainly used in pest prediction, interference mating, pest quarantine, and mass trapping.

SUMMARY OF THE INVENTION

The object of the present invention is to provide a composition for controlling Spodoptera frugiperda. Through structural modification of the sex pheromone components of Spodoptera frugiperda, corresponding pheromone analogs are synthesized, and the trapping quantity of Spodoptera frugiperda is further increased by adding a trace amount of sex pheromone analogs to the sex pheromone.

Based on the above purpose, the present invention provides a sex pheromone composition for controlling Spodoptera frugiperda, including sex pheromone and sex pheromone analogs, and the sex pheromone is cis-7-dodecene acetate, cis- -9-Tetradecene acetate and cis-11-hexadecen-1-ol, the sex pheromone analog is cis-7-dodecene-N-methylcarbamate, cis-7 -One or more of dodecene-N,N-dimethyl carbamate and cis-7-dodecene-N-methyl-N-ethyl carbamate.

The mass ratio of the sex pheromone to the sex pheromone analog is 1:(0.01-0.1).

The mass ratio of cis-7-dodecene acetate, cis-9-tetradecene acetate and cis-11-hexadecen-1-ol in the sex pheromone is 3:1 :2.

Optionally, the mass percentage of each component in the sex pheromone analog is: cis-7-dodecene-N-methylcarbamate 20%-40%, cis-7-dodecyl Alkene-N,N-dimethylcarbamate 32%-75%, cis-7-dodecene-N-methyl-N-ethylcarbamate 0%-35%.

Preferably, the mass percentage of each component in the sex pheromone analog is: cis-7-dodecene-N-methylcarbamate 25%-40%, cis-7-dodecyl Alkene-N,N-dimethylcarbamate 40%-55%, cis-7-dodecene-N-methyl-N-ethylcarbamate 10%-30%.

Preferably, the mass percentage of each component in the sex pheromone analog is: cis-7-dodecene-N-methylcarbamate 30%, cis-7-dodecene-N, N-Dimethylcarbamate 50%, cis-7-dodecene-N-methyl-N-ethylcarbamate 20%.

The present invention also provides a lure core of the composition for controlling Spodoptera frugiperda, the lure core containing sex pheromone and sex pheromone analogs.

The invention also provides the application of the composition for preventing and controlling Spodoptera frugiperda in controlling Spodoptera frugiperda, and preparing lure cores containing sex pheromone and sex pheromone analogs, each lure core containing 600-800 μg of the composition, The above-mentioned lure cores are placed in a barrel trap and placed in an area where Spodoptera frugiperda occurs seriously.

Optionally, the amount of the lure core is 400m ² /piece.

Preferably, the amount of the lure core is 200m ² /piece.

The beneficial effects of the present invention are as follows: the composition for controlling Spodoptera frugiperda of the present invention can more effectively trap and kill Spodoptera frugiperda males, reduce the frequency of mating, and reduce the reproduction rate. The composition is highly efficient in trace amounts, has strong specificity, is non-toxic to humans and animals, is environmentally friendly, and is harmless to natural enemies. The sex pheromone composition of Spodoptera frugiperda provided by the invention can be accurately applied to trap and interfere with mating of Spodoptera frugiperda, and is of great significance for comprehensively controlling Spodoptera frugiperda.

Description of drawings

Figure 1 is a schematic diagram of the chemical structure of cis-7-dodecene-N-methylcarbamate.

Figure 2 is a schematic diagram of the chemical structure of cis-7-dodecene-N,N-dimethylcarbamate.

Figure 3 is a schematic diagram of the chemical structure of cis-7-dodecene-N-methyl-N-ethyl carbamate.

Figure 4 is a synthetic route diagram of cis-7-dodecene-N,N-dimethylcarbamate.

Detailed ways

The specific embodiments of the present invention will be described in detail below with reference to the accompanying drawings, but it should be understood that the protection scope of the present invention is not limited by the specific embodiments.

Example 1 Preparation of cis-7-dodecene-N,N-dimethylcarbamate:

The synthetic route is shown in Figure 4. 0.5g Z7-12:OH (2.71mmol) was placed in a 100mL round-bottomed single-neck flask, and 15mL of re-distilled N,N-dimethylformamide (DMF) was added, 0.56g (4.07 mmol) acid binding agent K ₂ CO ₃ . A solution of DMF in dimethylcarbamoyl chloride (4.07 mmol) was slowly added dropwise. After dripping, the reaction was carried out at room temperature overnight, and the reaction process was monitored by TLC. The reaction was quenched with deionized water, extracted with ethyl acetate (10 mL×3), the extract was washed with saturated brine (15 mL×3), dried over anhydrous magnesium sulfate, concentrated under reduced pressure, and the crude product was purified by silica gel column chromatography to obtain cis-7-dodecene-N,N-dimethylcarbamate.

cis-7-dodecene-N,N-dimethylcarbamate (Z)-dodec-7-en-1-yldimethylcarbamate, pale yellow oily liquid, yield 62%; ¹ H NMR (300MHz, CDCl ₃ ) δ: 5.34-5.30(m, 2H), 3.91-3.89(t, J=17.4Hz, 2H), 2.89(s, 6H), 2.06-2.01(m, 4H), 1.61-1.54(m, 2H) ), 1.47-1.36 (m, 10H), 0.90 (t, J=9.3Hz, 3H); ¹³ C NMR (75MHz, CDCl ₃ )δ: 154.6, 130.3 (2C), 67.9, 37.4-37.2 (2C), 32.0, 29.4-28.7 (3C), 25.7, 22.3, 14.0; HR-MS (ESI) m/z: Calcd for _{C15H30NO2 {} [M+H] ⁺ } _256.2231 , found 256.2219.

Example 2 Field Biological Activity Determination Test

Preparation of lure:

The n-hexane solution containing sex pheromone was added to the anti-mouth rubber head according to the proportion to obtain the sex pheromone lure core (SP) containing Spodoptera frugiperda. Then add different sex pheromone analog components to the lure core, and after the solution completely penetrates into the trans rubber head, add 100 μL of n-hexane, and after the solvent is completely evaporated, it is made to contain sex pheromone and sex pheromone analogs 's lure. Each of the above-mentioned bait treatments was set up with 5 repetitions, and the bait blank treatment (CK) as a control and the barrel traps containing three live female Spodoptera frugiperda (PE) that had not been mated for 3 to 5 days after eclosion were 5 each. repetitions.

The prepared lure core was placed in a barrel trap, and placed in the area where Spodoptera frugiperda occurred seriously. In order to further illustrate the feasibility of the present embodiment, 22 specific formulas are provided in Table 1:

Table 1 Treatment numbers for different formulations

Select the area seriously harmed by Spodoptera frugiperda, with an area of more than 20 mu, the height of the lure core is about 120cm, and the interval between traps is about 20m. Three days after the placement, the number of trapped Spodoptera frugiperda was investigated once, and the trapping location was changed randomly for 30 days. The survey results are shown in Table 2:

Table 2 The trapping test results of different formulations on Spodoptera frugiperda

Different lowercase letters indicate significant difference at P<0.05 level in Duncan's test of significance

As can be seen from the results in Table 2, the composition of the present invention has a good trapping effect on Spodoptera frugiperda, containing cis-7-dodecene-N-methyl carbamate, cis-7-dodecene- The trapping amount of N,N-dimethylcarbamate, cis-7-dodecene-N-methyl-N-ethylcarbamate treatment compared with the trapping amount of Spodoptera frugiperda sex pheromone There is a significant difference (P<0.05), indicating that cis-7-dodecene-N-methylcarbamate, cis-7-dodecene-N,N-dimethylcarbamate, Cis-7-dodecene-N-methyl-N-ethylcarbamate has a synergistic effect on the sex pheromone activity of Spodoptera frugiperda, among which X11 has the most obvious synergistic effect, and the average trapping amount is 385.67± 7.86 heads/trap.

The sex pheromone analogue in the sex pheromone composition of Spodoptera frugiperda provided by the invention is simple to prepare, and the raw materials are cheap and easy to obtain; after adding the pheromone analogue to the pheromone, it has obvious synergistic effect on the sex pheromone components , harmless to humans and animals, environmentally friendly; strong resistance to stress, easy to use. The invention can be accurately applied to the insect condition forecasting of Spodoptera frugiperda, interfering with mating and trapping in large quantities, and is of great significance for comprehensively controlling Spodoptera frugiperda.

The above disclosures are only specific embodiments of the present invention, but the present invention is not limited thereto, and any changes that can be conceived by those skilled in the art should fall within the protection scope of the present invention.

Claims (9)

1. a composition for the prevention and treatment of Spodoptera frugiperda, is characterized in that, comprises sex pheromone and sex pheromone analogue, and described sex pheromone is cis-7-dodecene acetate, cis-9 -Tetradecene acetate and cis-11-hexadecen-1-ol, the sex pheromone analog is cis-7-dodecene-N-methylcarbamate, cis-7 -One or more of dodecene-N,N-dimethyl carbamate and cis-7-dodecene-N-methyl-N-ethyl carbamate. 2. the composition for preventing and treating Spodoptera frugiperda according to claim 1, is characterized in that, the mass ratio of described sex pheromone and sex pheromone analogue is 1:(0.01～0.1). 3. the composition for preventing and treating Spodoptera frugiperda according to claim 1, is characterized in that, the mass ratio of described sex pheromone and sex pheromone analogue is 1:(0.01～0.1); Described sex pheromone The mass ratio of cis-7-dodecene acetate, cis-9-tetradecene acetate and cis-11-hexadecene-1-ol is 3: 1: 2; the described The mass percentage of each component in the sex pheromone analog is: cis-7-dodecene-N-methyl carbamate 20%-40%, cis-7-dodecene-N,N- Dimethyl carbamate 32%-75%, cis-7-dodecene-N-methyl-N-ethyl carbamate 0%-35%. 4. the composition for preventing and treating Spodoptera frugiperda according to claim 1, is characterized in that, the mass ratio of described sex pheromone and sex pheromone analogue is 1:(0.01～0.1); Described sex pheromone The mass ratio of cis-7-dodecene acetate, cis-9-tetradecene acetate and cis-11-hexadecene-1-ol is 3: 1: 2; the described The mass percentage of each component in the sex pheromone analog is: cis-7-dodecene-N-methyl carbamate 25%-40%, cis-7-dodecene-N,N- Dimethyl carbamate 40%-55%, cis-7-dodecene-N-methyl-N-ethyl carbamate 10%-30%. 5. the composition for preventing and treating Spodoptera frugiperda according to claim 1, is characterized in that, the mass ratio of described sex pheromone and sex pheromone analogue is 1:(0.01～0.1); Described sex pheromone The mass ratio of cis-7-dodecene acetate, cis-9-tetradecene acetate and cis-11-hexadecene-1-ol is 3: 1: 2; the described The mass percentage of each component in the sex pheromone analog is: cis-7-dodecene-N-methyl carbamate 30%, cis-7-dodecene-N,N-dimethyl Carbamate 50%, cis-7-dodecene-N-methyl-N-ethylcarbamate 20%. 6 . The lure of the composition for controlling Spodoptera frugiperda according to claim 1 , wherein the lure contains sex pheromone and sex pheromone analogs. 7 . 7. the application of the composition for controlling Spodoptera frugiperda described in any one of claims 1-5 in controlling Spodoptera frugiperda. 8. according to the application of the composition for preventing and treating Spodoptera frugiperda according to claim 7, it is characterized in that, make the described lure core of claim 6, and each lure core contains 600-800 μg Composition, the above-mentioned lure core is placed in a barrel trap, and placed in an area where Spodoptera frugiperda occurs seriously. 9. the application of the composition for controlling Spodoptera frugiperda according to claim 8 in controlling Spodoptera frugiperda, it is characterized in that, the consumption of described lure core is 200 m ² /.

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