CN1113665C - Inflammation diminishing and stone dissolving and exhausting liquid and its production process - Google Patents

Abstract

The present invention provides inflammation diminishing and stone dissolving and exhausting liquid, which solves the defects that the preparation progress of the traditional medicine is complicated; the cost is high, and ordinary patients are difficult to bear. The present invention is composed of emodin and turpentine oil. The present invention has the advantages of reasonable formula, advanced extraction process, high therapeutic effects, small toxicity and side effects, wide medicinal herbs resources and low price.

Description

Antiinflammatory, stone dissolving and exhausting liquid and preparation method thereof

The present invention relates to treat Iithiasic antiinflammatory, stone dissolving and exhausting liquid and preparation method thereof.

Calculus is a kind of commonly encountered diseases, as cholelithiasis, renal calculus, traditional therapy has operation and two kinds of oral drugs, because the inconvenience of performing the operation, the patient selects oral medicine usually, existing oral medicine, complicated process of preparation, cost height, often several thousand yuan of courses of treatment, general patient is difficult to bear, and its therapeutic effect is not good.

The objective of the invention is provides a kind of reasonable recipe, extraction process advanced person, curative effect height, toxic and side effects is little, the medicine source is wide, cheap antiinflammatory, stone dissolving and exhausting liquid in order to overcome the shortcoming of existing medicine bottle.

For achieving the above object, the present invention is by the following technical solutions:

A kind of antiinflammatory, stone dissolving and exhausting liquid, its prescription consists of: emodin, Oleum Terebinthinae.

The preparation method of this antiinflammatory, stone dissolving and exhausting liquid, concrete processing step is:

(1) Radix Et Rhizoma Rhei extracts: (1) Radix Et Rhizoma Rhei decoction piece soaks after 24 hours with 5 times of amounts of 2% potassium nitrate, 70% ethanol, 50 ℃ of reflux, extract, of low temperature, each 10-15 minute; Continuous dipping extracts 3 times with method, merges 3 times extracting solution, reclaims ethanol, and concentrate under reduced pressure at low temperature becomes cream; (2) add 3 times of amounts of 70% ethanol again, smash all, leave standstill 12-14 hour precipitated impurities, extract supernatant liquid filtering; Precipitated liquid is with 45% washing with alcohol 2-3 time, and the solubility composition washes out in will precipitating, and filtrate and washing liquid merge, and reclaim ethanol, the cold true one-tenth cream of concentrating under reduced pressure.

(2) Oleum Terebinthinae extraction process: common medical Oleum Terebinthinae redeposition is filtered, and filtrate is contained in the bottle, and the bottle sealing is preserved.

(3) antiinflammatory, stone dissolving and exhausting liquid preparation process: emodin 80mg/kg/ day, Oleum Terebinthinae 0.4ml/kg/ day, mix and add in antiseptic Tween 80, sweetener, two 10ml oral liquids of the packing coloured glass bottle sealing preservation.

The present invention has following characteristics and effect:

One, the medicine characteristics of antiinflammatory stone dissolving and exhausting liquid:

1, this medical instrument has novelty, reasonable recipe: be made up of Radix Et Rhizoma Rhei, Oleum Terebinthinae: have high-efficiency low-toxicity, characteristics that toxic and side effects is little; We increase IBP with antiinflammatory relieving spasm to stop pain, lithodialysis, calculus, increase bile secretion, function of gallbladder promoting, expansion Ao Dishi sphincter and discharge cholelithiasis.Antiinflammatory relieving spasm to stop pain, lithodialysis, calculus increase the urine flow and discharge kidney, ureter, vesical calculus.(1) efficient: as to control cholelithiasis 236 examples altogether, cure 169 examples and account for 71.61%; Produce effects 42 examples account for 17.80%; Effective 21 examples account for 8.90%; Invalid 4 examples account for 1.70%; Total effective rate 98.30%.(2) quick-acting: as after taking medicine 2 days, calculus 121 people to occur and account for 51.27%.(3) nontoxic: to the heart, liver, kidney, the equal avirulence infringement of brain.(4) side effect: it is the calculus reaction that part patient has diarrhoea, slight biliary colic, ureteral colic.

2, our extraction process advanced person: Radix Et Rhizoma Rhei with 2% potassium nitrate, 5 times the amount 70% ethanol, soak after 24 hours, low temperature reflux extracts, the response rate reaches 42%, the purity height, and curative effect improves 46% than similar medicine, serum potassium increases, increased nervous irritability, increase bile secretion, potassium nitrate itself just has the lithodialysis laxative action, and potassium is removed inhibition and the infringement of Radix Et Rhizoma Rhei Senna fruit glycoside to cardiac muscle again.

3, this side has very strong litholytic effect, and Oleum Terebinthinae all has very strong litholytic effect to cholesterol calculus, bilirubin calculus etc.Especially to pigmented calculus 100% dissolving in 60 hours.Radix Et Rhizoma Rhei extracts potassium salt laxative in the solution, and very strong calculus dissolving and excreting effect is also arranged.Reduce bile cholesterol saturation effect, make bile cholesterol be in dissolved state and be difficult for separating out crystallization, the cholesterol calculus dissolving.Adjust normal lipid metabolism in the bile, the control cholesterol calculus forms.Reduce cholesterol and from intestinal, absorbs, reduce HMG-COA reductase activity in the hepatocyte, thereby the synthesizing and from bile, discharge of inhibition cholesterol.Increase the secretion of bile acid and lecithin, the control cholesterol calculus forms.

4, cholagogic and lithagogue effect: promote bile secretion, increase IBP, bilirubin and bile acid content are increased, bile flow obviously increases, and the expansion of Ao Dishi sphincter helps cholagogic and lithagogue.

5, antiinflammatory relieving spasm to stop pain, diuresis and expelling stone effect: dissolving urinary system calculus (calcium oxalate stone, calcium phosphate stone, magnesium ammonium phosphate calculus etc.), kill the urinary system bacterial infection, diminish inflammation, remove the painful contraction of ureter, increase the urine flow, reach diuresis and expelling stone.Control urinary system calculus 257 examples altogether.Ureteral calculus 176 examples wherein, renal calculus 27 examples, multiple calculus 18 examples, vesical calculus 12 examples, calculus of urethra 2 examples, 4 examples are failed to understand at the position.Cure calculus and calculus shadow 186 examples that disappear and account for 72.37%; Produce effects 38 examples account for 14.79% and account for; Effective 25 examples 9.73%; Invalid 8 examples account for 3.11%, total effective rate 96.89%.

6, very strong antibiotic, antifungal, antiviral, parasiticide effect are arranged: remove mucus piece, antibacterial, the epithelial cell that comes off, inflammation exudate in the gallbladder, parasite, foreign body etc. prevents that calculus from forming.

7, very strong anti-inflammatory analgesic effect is arranged: elimination antibacterial, virus, pathogenic microorganism cause ulcer hyperemia, edema inflammatory reaction to gallbladder, biliary pore mucosa, and granulation tissue hyperplasia, and the congestion and edema inflammatory reaction of inhibition and tissue repair effect are arranged.

8, hepatoprotective effect: get rid of hepatic calculus, hepatic injury is had protective effect.

9, anti-tumor of biliary tract and urological cancer effect, immune dual regulation.

10, the effect of stopping blooding, invigorate blood circulation: control gallbladder system and urinary system is hemorrhage, the thrombus dissolving effect.Blood vessel dilating and hypotensive effect.

11, promote pancreatic secretion: prevent acute edema type or downright bad pancreatitic generation of type of acute hemorrhage and development that trypsin or ethanol bring out; And trypsin, pancreatic lipase, pancreatic amylase activity there is obvious inhibitory action.Suppressing three kinds of enzymes is the pancreatitic bases of treatment, and pepsin activity is not had influence.Helping biliary tract is that inflammation is eliminated, and calculus is discharged, the reparation as early as possible of tissue.

The pharmacological action of two, antiinflammatory, lithodialysis, calculus removal liquid:

The Radix Et Rhizoma Rhei pharmacological action

(1) choleretic effect: Radix Et Rhizoma Rhei can promote the bile secretion of Canis familiaris L., and the content of bilirubin and bile acid is increased.Rat experiment proves that big yellow fluid, alcohol extract, Radix Et Rhizoma Rhei decoct have all made bile flow obviously increase, and its peak is in administration 30 minutes.Observe to find to take with B ultrasonic give birth to, behind the Radix et Rhizoma Rhei (processed) 0.5 and 1 hour gallbladder obviously greater than before the clothes Radix Et Rhizoma Rhei, also can promote to test the biliary secretion of acute suppurative cholecystitis, the composition that the prompting Radix Et Rhizoma Rhei is significantly increased bile secretion also has it to suppress composition, and this preparation research and clinical practice for Radix Et Rhizoma Rhei provides certain clue.

(2) discharge function: Radix Et Rhizoma Rhei has discharge function, is used for the treatment of constipation due to dry stool, constipation with heat retention, generally can discharge loose stool in 6-19 hour after medication.The Senna fruit glycoside is the effective ingredient of Radix Et Rhizoma Rhei diarrhea inducing, and Radix Et Rhizoma Rhei has excited and suppresses the gastrointestinal dual function, and the material base of this effect is exactly Senna fruit glycoside and tannin class.

(3) to the influence of gastric and duodenal ulcers: Radix Et Rhizoma Rhei can be treated and be prevented the hemorrhage of stress gastric ulcer, shows as extent of hemorrhage and obviously alleviates, and hemorrhagic focus, the duty of hemorrhagic focus face obviously reduce, and its effect is similar to cimetidine.

(4) to the influence of intestinal tube electrical activity and contraction movement: Radix Et Rhizoma Rhei has tangible excitation to the electrical activity of whole colon, characteristics are the gregariousness discharge, peak electricity frequency obviously speeds, amplitude obviously increases, this explanation Radix Et Rhizoma Rhei hinders the colonic moisture absorption by excited intestinal electrical activity, accelerates the discharge of colonic contents and brings into play its discharge function, and low dose makes the inhibition of colon electrical activity, heavy dose makes the excitement of intestinal electrical activity, then occurs excited earlier back sometimes and suppresses phenomenon.Bring into play its discharge function by the m receptor that acts in the intestinal tube.

(5) to the influence of liver: Radix Et Rhizoma Rhei has significant protective effect to experimental hepatic injury.Radix Et Rhizoma Rhei can reverse significantly that the fat that occurs in the hepatic tissue that CCL4 causes drips and fibrosis, microsome swelling, ridge obviously descend, rough endoplasmic reticulum destroys, ribosome significantly comes off etc.The hepatic injury that Radix Et Rhizoma Rhei causes CCL4 truly has prevention and therapeutical effect.

(6) promote the pancreatic secretion effect: experimental results show that acute edema type or downright bad pancreatitic generation of type of acute hemorrhage and development that Radix Et Rhizoma Rhei can prevent that trypsin or ethanol from bringing out.Tissue hyper-microstructure confirms that it also has the obvious treatment effect to the pancreas in rat inflammation that D-two methyllanthionines cause.In 30 minutes, pancreatic juice all obviously increases after administration for Radix Et Rhizoma Rhei decoct and preparation, and dosage strengthens, and effect is strengthened, and it is more of a specified duration to hold time.The Radix Et Rhizoma Rhei decoct in vitro reaches under the nearly gastrointestinal tract environment of simulation, and the activity of trypsin, pancreatic lipase and pancreatic amylase is had the obvious suppression effect, and to the not influence of pepsic activity.

Radix Et Rhizoma Rhei and extract thereof have the effect of significant promotion pancreatic secretion, may be relevant with the lax Ao Dishi sphincter tone of Radix Et Rhizoma Rhei, and Radix Et Rhizoma Rhei may be the pancreatitic basis of rhubarb treatment to the influence of three kinds of digestive enzyme of pancreatic secretion.

2, to the influence of pathogenic microorganism:

(1) antibacterial action: experimental results show that Radix Et Rhizoma Rhei all has in various degree inhibitory action to staphylococcus, Hemolytic streptococcus, diphtheria corynebacterium, bacillus subtilis, Mycobacterium phlei, brucella, bacillus pestis, typhoid fever, Salmonella paratyphi, dysentery bacterium, deep shape bacillus, bacillus smegmatis, gonorrhea diplococcus etc., the most responsive with staphylococcus, gonorrhea diplococcus especially.3-carboxyl rhein, hydroxyl aloe-emodin, hydroxyemodin three antibacterial action are the strongest.Chrysophanic acid, emodin, aloe-emodin are 1.5-25mcg/ml to the inhibition concentration of staphylococcus, streptococcus, diphtheria corynebacterium, bacillus subtilis, anthrax bacillus, Bacillus typhi etc.Emodin, aloe-emodin are respectively 15mg/l, 480mg/l, 120mg/l and 7.5mg/l, 600mg/l, 60mg/l to staphylococcus aureus 209P, escherichia coli, shigella flexneri at external minimum inhibitory concentration.To clinical isolating 119 strain staphylococcus aureus minimum inhibitory concentrations, emodin is 20-160mg/l, and aloe-emodin is 10-80mg/l.After the inducible resistance test, aloe-emodin produces drug resistance, and emodin does not produce drug resistance.Radix Et Rhizoma Rhei antibacterial action mechanism mainly is to bacterial cell nucleic acid and protein synthesis and glycometabolic inhibitory action.

(2) antifungic action: the extractum of Radix Et Rhizoma Rhei decoct and water, alcohol, ether has inhibitory action to some common pathogenic funguses in vitro, and responsive strain has oidium schoenleinii and Mongolian mutation thereof, Trichophyton concentricunm, trichophyton, star nocardia, sufficient sole of the foot trichophyton, acrothesium floccosum, trichophyton gypseum, trichophyton interdigitale, sporotrichum schenckii etc.

(3) antivirus action: the susceptible poison of Radix Et Rhizoma Rhei decoct convection current has stronger inhibitory action.

(4) parasiticide effect: the Radix Et Rhizoma Rhei leachate can kill histolytica distortion protozoon respectively in the concentration of 1: 5000 and 1: 1000, can kill Trichomonas hominis at 1: 5000, and a little less than the inhibition to intestinal infusorian and Wan Shi lip whipworm.Emodin has 40% suppression ratio to the mouse infection schistosomicide.The concentration of wild Radix Et Rhizoma Rhei 1% can make cercaria dead in 90 minutes.5% Radix Et Rhizoma Rhei leachate can make culex pipiens pallens larvae dead 50% in 48 hours.

3, to the influence of cardiovascular system:

(1) to the influence of heart: the emodin low dose is strengthened the isolated heart contractility, and heavy dose then suppresses.Radix Et Rhizoma Rhei can make the MAP amplitude of heart increase, and climbing speed speeds, and myocardial contraction obviously strengthens, and proves that Radix Et Rhizoma Rhei has significantly cardiotonic.Increase that K+ concentration can weaken the toxic action of heavy dose of Radix Et Rhizoma Rhei to heart in the extracellular fluid, the cardiotonic of Radix Et Rhizoma Rhei may with the Na+ that suppresses on the cell membrane, the K+-ATP enzyme is relevant.Rhubarb polyoses can make the normal rat blood pressure drops, and decreased heart rate makes normal and depleted Bufo siccus cardiac contractile force and the cardiac output of exsomatizing increases, the prompting cardiotonic may be by suppress Myocardial Na+, the K+-ATP enzyme is realized.

(2) to the influence of blood vessel: Radix Et Rhizoma Rhei preserved material, tincture and emodin all have the effect that brings high blood pressure down.The Radix Et Rhizoma Rhei tincture can make rabbit ear vasodilation.

(3) stop blooding and the effect of invigorating blood circulation: the hemostasis effective ingredient α-catechin of Radix Et Rhizoma Rhei and gallic acid can reduce the activity of antithrombase II (AT II), the content of rising α 2-MG (alpha2 Macroglobulin), the vigor that suppresses fibrinolysin and plasminogen activin competitively, the fibrinolytic vigor is descended, and the adhesiveness of platelet increasing and ability of aggregation, thereby quicken hemostasis.

(4) blood fat reducing and antiobesity action: rhubarb polyoses can make egg yolk and the inductive hyperlipidemia mice serum of high lipid food and total cholesterol of liver (TC), triglyceride (TG) obviously reduce, may be relevant with anorexia and cathartic.There is the effect of tangible reduction serum total cholesterol at the alcohol extraction position of Radix Et Rhizoma Rhei.Living, Radix Et Rhizoma Rhei has tangible antiobesity action, and its blood fat reducing and fat-reducing composition may be anthraquinone class, catechin compounds, and polysaccharide also has these effects.

4, the chrysophanic acid of antitumor action: 5mg/kg and emodin are respectively 76% and 73% to the suppression ratio of murine melanoma.Emodin has significant competitive inhibition to cheese ammonia enzyme, and this inhibitory action may be one of mechanism of action of Radix Et Rhizoma Rhei melanoma.

5, to Immune Effects: emodin and aloe-emodin lumbar injection 70mg/kg7d have in various degree inhibitory action to normal mouse immune system, as alleviate the weight of immune organ, reduce production of antibodies, suppress carbon clearance function and peritoneal macrophage phagocytic function, reduce leukocyte count, delayed hypersensitivity due to inhibition 2, the 4 dinitro phenalgins (DNCB).

6, to the influence of kidney: Radix Et Rhizoma Rhei has the effect of non-protein nitrogen in the tangible reduction blood, this effect may be owing to reduced the absorption of intestinal to amino nitrogen (raw material of urea synthesis), and essential amino acids concentration is raise, utilize the catabolite of vivo acid---ammonia synthesis protein, thus liver, nephridial tissue urea synthesis are reduced; Radix Et Rhizoma Rhei also suppresses the decomposition of body protein, with the content of minimizing blood urea nitrogen and creatinine, and the drainage of promotion carbamide and creatinine.

7, antiinflammatory action: Radix Et Rhizoma Rhei has the obvious suppression effect to multiple zoopery inflammation, and oral decoct can significantly suppress Oleum Tiglii and cause mice ear inflammatory edema, rat formaldehyde, Ovum Gallus domesticus album pedal swelling, the granuloma induced by implantation of cotton pellets hypertrophy of mice and rat.Rheum hotaoense C. Y. Cheng et C. T. Kao extractum all has significant inhibitory effect to rat Ovum Gallus domesticus album and formaldehyde pedal swelling and mouse experiment peritonitis.

8, analgesic cooling effect: after gavaging the Radix Et Rhizoma Rhei water decoction for the rabbit of normal and Diplococcus pneumoniae infectious fever, observe pathologic fever in rabbit body temperature (anus temperature) and obviously descend, simultaneously as seen in the ventriculus tertius perfusate PGE content obviously reduce.Radix Et Rhizoma Rhei may be brought into play cooling effect by influencing PGE.

9, anti-aging effects: the rheum rhabarbarum polysaccharide that extracts in the rheum rhabarbarum can obviously prolong average life and the maximum life span of fruit bat.The rheum rhabarbarum polysaccharide also can increase swimming time and the hypoxia-bearing capability of 2-3 monthly age mice, shows that the rheum rhabarbarum polysaccharide has multiple effects such as strong.

10, to the influence of diabetes: the type i diabetes rat drug resistance to insulin disappearance that Radix Et Rhizoma Rhei can make streptozotocin cause, ERYTHROCYTE INSULIN maximum combined power raises, and serum insulin levels reduces.Radix Et Rhizoma Rhei has similar effect to alloxan diabetes rats.Radix Et Rhizoma Rhei can improve the Insulin receptor INSR adhesion, improves sugar, lipoidosis and hyperinsulinemia.Illustrating that acceptor binding force reduces and the information conductive impairment is the basis of development of insulin resistance, show that Radix Et Rhizoma Rhei is at acceptor levels treatment NIDDM, is the atherosclerotic ideal medicament of prevent diabetes.

11, estrogen-like effects: emodin has estrogen-like effects, can make the rapid restorative cycle of castration female rats, and clinic trial also has the effect of estrone sample.

12, diuresis: Radix Et Rhizoma Rhei crude drug and chrysophanic acid, emodin all have diuresis.

13, physiological disposition:

(1) absorb: adopt the absorption of isotope-labelling method research emodin, the single oral dose emodin, radioactivity increases sharply in the PBF, reaches maximum in 2 hours, then drops to peakedly 30% in 24 hours, then slowly descends later on.(2) distribute: after rhubarb anthraquinone absorbs, serve as maximum with liver, kidney, gallbladder in vivo, isotope-labelling method proves that emodin mainly is distributed in kidney, mesentery and the fat, in muscle, brain, small intestinal and the colon seldom.(3) drain: rhubarb anthraquinone is discharged by excrement and urine, accounts for 23.4% and 22.8% of intake respectively, and it serves as maximum discharging with 2-4 hour through urine, discharges about 61% in 8 hours.

14, toxic and side effects

(1) acute toxicity: the injection of Radix Et Rhizoma Rhei ether extract mouse peritoneal, dosage reaches 40g (crude drug)/kg, observes 72 hours, does not see dead and unusual.Rhubarb preparation mouse subcutaneous injection LD50 is 4.052g/kg and 2g/kg.With age, CPC was every group of 6 lumbar injections of mice all around, the LD50 of the Diluted Alcohol extractum of Radixet Rhizoma Rhei, Rheum tanguticum, second-class Rheum tanguticum is 0.25-0.5g/kg; The Rheum tanguticum decoction pieces, etc. the about 0.25g/kg of LD50 of outer Radix Et Rhizoma Rhei; The Radix Et Rhizoma Rhei oral toxicity is injected little.The LD50 of Radix Et Rhizoma Rhei anthraquinone analog derivative mouse stomach, emodin are that 0.56g/kg, physcione are 1.15g/kg, and chrysophanol is 10g/kg.

(2) long term toxicity:

1. gastrointestinal is influenced: irritate the stomach rat with 200% Radix Et Rhizoma Rheidecocted solution, every day 3-4.5ml, scanning electron microscopic observation has the different irritable inflammation of degree to its harmonization of the stomach large intestine after 4,5 days, thinks that this is to cause indigestion and loss of appetite, diarrheal reason.2. to the immunologic function inhibitory action: have the report Radix Et Rhizoma Rhei to irritate " deficiency syndrome " rat due to the stomach, immune organs such as thymus, spleen, mesenteric lymph node diminish, alleviate, and also cause the obvious atrophy of thymus in mice, Golden Hamster.3. to the influence of reproductive system: Radix Et Rhizoma Rhei was irritated the stomach rat 7.5g/kg14 days, and visible female Mus period of sexual maturity obviously delays, and uterus, ovary weight alleviate.

The Oleum Terebinthinae pharmacological action

(1) effect of Oleum Terebinthinae character and usage: be colourless or faint yellow clear liquid.Smelly special, store with the passing of time or be exposed at that foul smell strengthens, is dissolved in ethanol, can mixes arbitrarily with glacial acetic acid in the air, skin irritation, sterilize, draw red effect, preparation kentish liniment, be used for myalgia, rheumatic arthritis, peripheral neuritis, sprain, erythema nodosum, anaphylactoid purpura etc. are to 60 hours lithodialysis rates 100% of bile pigments type calculus.

(2) Oleum Terebinthinae litholytic effect:

Experiment in vitro confirms that Oleum Terebinthinae Emulsion can make the cholelithiasis dissolving of taking from the cholelithiasis patient, make bile pigments type calculus add all dissolvings in back 60 hours at Emulsion, so Oleum Terebinthinae Emulsion has good litholytic effect.

(3) antibiosis and antiviral functions:

1, antibacterial action

The ammonia extraction thing of Pinus koraiensis is to mouse peritoneal or intravenously administrable, the animal dead that escherichia coli, pneumobacillus, staphylococcus aureus are caused has tangible antagonism, and different alkaline extracts also have significant antibacterial activity to the mouse infection that staphylococcus aureus, escherichia coli, Pseudomonas aeruginosa, pneumobacillus, Candida albicans cause.The generation that antibacterial activity thing intraperitoneal administration can be induced differentiation inducing factor (DIF), after the administration 1-2 hour, peak, and can make polymorphonuclear cell (PMN) accumulation that the generation of peritoneum emigrated cell and adherent cell superoxides is increased, the activation of PMN plays an important role in the pine extract antibacterial action.

2, anti-AIDS-virus effect

The extract PC6 of Pimus parviflora Sieb et Zucc is through experiment in vitro, can suppress duplicating of HIV, virus breeding is suppressed, reaching 80% Cmin that suppresses effect is 3mg/ml, HIV-1 in U937 people's lymphocytic cancer cell is also shown obvious inhibitory action, PC6 can suppress the proteic expression of HIV-1 in the cem cell, and PC6 can suppress HIV-1 transcribing and translating in cem cell, and gene expression also has inhibitory action to LTR guiding DNA at transcriptional level.PC6 can produce a kind of solable matter to the pepsin sensitivity by inducing T cell (CEM), this material is that PC6 produces the reason that inhibition HIV-1 duplicates effect, this PC6 inductive HIV-1 inhibitive factor effect and known granulocyte/macrophage activating factor (MAF) (GM-CSF), cytokine 3 (IL-3), β-transforming factor (TNF-β) is irrelevant with the effect of cachectin (TNF-α), so the inductive this anti-HIV-1 material of PC6 may be a kind of new immunologic active material.

3, resisiting influenza virus and other effect

Antibacterial activity composition lignin has the effect of resisiting influenza virus too.Can obviously suppress the growth of influenza virus, the plaque due to the influenza virus is reduced, can significantly suppress the synthetic of virus protein in the infection cell, reduce the activity of the RNA polymerase that viral RNA relies on.

(4) antitumor action:

Its anti-tumor activity size is relevant with acid power, the acid polysaccharide of NaOH extract wherein, and anti-tumor activity is the strongest, can obviously suppress the growth of sarcoma cell, even causes the degeneration and the necrosis of tumor.

(5) to the immune system effect:

Polysaccharose substance can activate mouse macrophage, and the hot water extract can improve the Fc receptor active, and acidic extraction thing energy significant stimulation mice and isolated rat splenocyte DNA's is synthetic, and this acidic extraction thing has immunologic enhancement.

(6) toxic and side effects:

Siberian dwarf pine leaf volatile oil mice LD50 is 0.577 ± 0.056ml/kg; The fruit of Pinus pinea is oral to cause systemic allergic report.

Below in conjunction with embodiment the present invention is further described.

The prescription of this antiinflammatory, stone dissolving and exhausting liquid consists of: emodin, Oleum Terebinthinae.

Its preparation method is realized according to following processing step:

(1) Radix Et Rhizoma Rhei extraction process: (1) Radix Et Rhizoma Rhei decoction piece soaks after 24 hours with 5 times of amounts of 2% potassium nitrate, 70% ethanol, 50 ℃ of reflux, extract, of low temperature, each 10-15 minute; Continuous dipping extracts 3 times with method, merges 3 times extracting solution, reclaims ethanol, and concentrate under reduced pressure at low temperature becomes cream.The response rate reaches 42%.(2) for purity is higher, add 3 times of amounts of 70% ethanol again, smash all, leave standstill 12-14 hour precipitated impurities, extract supernatant liquid filtering; Precipitated liquid is with 45% washing with alcohol 2-3 time, and the solubility composition washes out in will precipitating, and filtrate and washing liquid merge, and reclaim ethanol, the cold true one-tenth cream of concentrating under reduced pressure.

(2) Oleum Terebinthinae extraction process: commercially available medical Oleum Terebinthinae redeposition is filtered, and filtrate is packed in the bottle, preserves the bottle sealing anti-oxidation to prevent.

(3) antiinflammatory stone dissolving and exhausting liquid preparation process

Use according to clinical repetition test and checking, select the best safety consumption for use: emodin 80mg/kg/ day, Oleum Terebinthinae 0.4ml/kg/ day, mix in adding antiseptic Tween 80, sweetener, two 10ml oral liquids of the packing coloured glass bottle, sealing is preserved.

Claims (2)

1. an antiinflammatory, stone dissolving and exhausting liquid, its component is: emodin, Oleum Terebinthinae, its composition are emodin 80mg, Oleum Terebinthinae 0.4ml.

2. the preparation method of an antiinflammatory, stone dissolving and exhausting liquid, concrete processing step is:

(1) Radix Et Rhizoma Rhei extracts: (1) Radix Et Rhizoma Rhei decoction piece soaks after 24 hours with 5 times of amounts of 2% potassium nitrate, 70% ethanol, 50 ℃ of reflux, extract, of low temperature, each 10-15 minute; Continuous dipping extracts 3 times with method, merges 3 times extracting solution, reclaims ethanol, and concentrate under reduced pressure at low temperature becomes cream; (2) add 3 times of amounts of 70% ethanol again, smash all, leave standstill 12-14 hour precipitated impurities, extract supernatant liquid filtering; Precipitated liquid is with 45% washing with alcohol 2-3 time, and the solubility composition washes out in will precipitating, and filtrate and washing liquid merge, and reclaim ethanol, the cold true one-tenth cream of concentrating under reduced pressure.

(2) Oleum Terebinthinae extraction process: common medical Oleum Terebinthinae redeposition is filtered, and filtrate is contained in the bottle, and the bottle sealing is preserved.

(3) antiinflammatory, stone dissolving and exhausting liquid preparation process:, mix and add in antiseptic Tween 80, sweetener, two 10ml oral liquids of the packing coloured glass bottle sealing preservation with the ratio of emodin 80mg, Oleum Terebinthinae 0.4ml.