CN111330001B - Suspending agent and suspension of rabbit coccidiosis attenuated live vaccine - Google Patents

Abstract

The invention relates to veterinary parasitology and immunobiology, in particular to a suspension agent and a suspension of a rabbit coccidiosis live attenuated vaccine. The rabbit coccidiosis live attenuated vaccine suspending agent contains a suspending compound with a mass fraction of 0.15-0.3‰, and the suspending compound is composed of pectin and yellow with a mass ratio of 1:(0.8-1.2). Original gum composition. The live attenuated rabbit coccidiosis vaccine suspending agent in the present invention can realize the immunization of rabbits by drinking water. In addition, the suspending agent has a fast dissolving speed at normal temperature, so that the rabbit coccidiosis oocysts can be uniformly dispersed in water. The system is maintained for more than 6 hours without sinking, which ensures the uniformity of the immunization of the live rabbit coccidiosis vaccine, and provides strong technical support for the promotion and application of the live attenuated rabbit coccidiosis vaccine in actual production.

Description

A kind of rabbit coccidiosis live attenuated vaccine suspending agent and suspension

technical field

The invention relates to veterinary parasitology and immunobiology, in particular to a suspension agent and a suspension of a rabbit coccidiosis live attenuated vaccine.

Background technique

Rabbit coccidiosis is a parasitic disease caused by Eimeria coccidia parasitizing the bile duct or intestinal tract. Among them, rabbit coccidiosis is the most common and most serious parasitic disease. There are many species of Eimeria in rabbits, and there are currently 11 species recognized internationally. The main popular species in my country are Eimeria magna, Eimeria media, and Eimeria intestinalis. Etc., all kinds of rabbits are susceptible to Eimeria, and rabbits aged 1-4 months are the most susceptible. The mortality rate of young rabbits is high after infection, and they become long-term carriers after they are resistant. They continuously discharge oocysts into the environment, contaminate equipment such as cages, feeding tanks and drinking water, and infect young rabbits. This cycle is an important reason why rabbit coccidiosis cannot be effectively controlled.

Animal diseases will restrict the development of the rabbit industry. As mentioned above, rabbit coccidiosis is one of the major problems to be solved urgently. Since there is currently no relevant vaccine on the market, only anti-coccidial drugs are used for prevention. However, the use of drugs has brought many negative effects, such as causing drug residues in animal food and causing the emergence of drug-resistant insect strains. Therefore, there is an urgent need for safer and more effective methods for the prevention and control of rabbit coccidiosis in actual breeding.

The great success of coccidiosis vaccines in chicken coccidiosis control demonstrates that the use of live oocyst vaccines is the best way to prevent coccidiosis. At present, there are some live attenuated coccidia vaccines with good safety and efficacy in the art, and a good immune effect can be obtained by inoculating the live attenuated coccidia vaccine. However, when this immunization method of vaccination is applied to actual farming, it takes a lot of time and human resources, which is not conducive to controlling production costs.

In contrast, drinking water immunization method is the best method for immunization of large-scale farms at this stage, and is suitable for complex breeding environment and production conditions. However, because the density of oocysts is greater than that of water, the oocysts in the vaccine tend to sink after standing for a long time, which affects the uniform distribution of oocysts in the water, resulting in uneven vaccine immunity and failure of immunity.

Existing chicken coccidiosis vaccines are mainly composed of one or more of Eimeria tenella, Eimeria pilosula, Eimeria giant, and Eimeria poisoning early maturing strains. The average size of oocysts was 22 μm×19 μm, 18 μm×15 μm, 31 μm×21 μm, and 20 μm×17 μm, respectively. The life history of rabbit coccidia is similar to that of chicken coccidia, but there are some differences in the size of oocysts. For example, in the live attenuated rabbit coccidiosis vaccine, the average size of the oocysts of Eimeria large is 37.3 μm×24.1 μm, that of Eimeria medium is 25.4 μm×15.3 μm, and that of Eimeria intestinalis is 27 μm× 18 μm, therefore when the coccidial vaccine suspension agent applied to chickens in the prior art is applied to the live attenuated coccidiosis vaccine in rabbits, due to the different sizes and densities of the oocysts, there will be certain differences in suspension stability, Therefore, it is necessary to prepare a suspending agent suitable for a live attenuated rabbit coccidiosis vaccine according to the oocyst characteristics of different species of rabbit coccidiosis. At the same time, the coccidia vaccine suspending agent currently used in chickens is relatively complex in components, and the required production cost is relatively high. In addition, the existing suspending agent applied to chicken also has the problem of poor solubility of the suspending agent. Since most colloids are relatively insoluble, a lot of time is wasted for dissolving the suspending agent in production and application.

SUMMARY OF THE INVENTION

(1) Technical problems to be solved

In order to find a suitable suspending agent, the coccidiosis vaccine was made into a stable suspension, the rabbit's drinking time was prolonged, and the rabbit's stress response was reduced, so that it could obtain uniform and effective coccidiosis immunity protection.

(2) Technical solutions

In the first aspect, the present invention provides a suspending agent for a live attenuated rabbit coccidiosis vaccine, which contains a suspending compound with a mass fraction of 0.15-0.3‰ (more preferably 0.25‰), and the suspending compound is composed of The mass ratio is 1:(0.8-1.2) pectin and xanthan gum.

It is found in the present invention that the above-mentioned suspending agent can not only maintain the suspended state of vaccine oocysts in the vaccine solution, but also not adversely affect the yield of oocysts and sporozoites, and at the same time, it is non-toxic to rabbits, which is an ideal Rabbit coccidiosis live attenuated vaccine suspension.

Preferably, the mass ratio of the pectin and xanthan gum is 1:1.

The present invention further finds that a certain concentration of KCl can increase the dissolution rate without affecting the effect of the above-mentioned suspending compound. 1.2% (more preferably 1.0%) KCl.

In addition, in the process of research and development, the present invention has also tried to use the material drying powder in CN200510036004.6 and the heating method in CN201210231082.1, etc., all of which cannot make pectin and xanthan gum dissolve quickly.

Preferably, the pectin, xanthan gum and KCl are all food grade.

Of course, other raw materials (such as water) used in the suspension of the live attenuated rabbit coccidiosis vaccine should also be food grade or pharmaceutical grade.

In some embodiments, the preparation method of the rabbit coccidiosis live attenuated vaccine suspension preparation is as follows: take pectin, xanthan gum and KCl respectively by mass, mix with water, stir and mix well.

In a second aspect, the present invention provides a suspension of live attenuated rabbit coccidiosis vaccine, comprising the live attenuated rabbit coccidiosis vaccine and the said live attenuated rabbit coccidiosis vaccine suspending agent.

Preferably, when the preparation raw material of the live attenuated rabbit coccidiosis vaccine is Eimeria large, Eimeria medium and Eimeria enterica precocious line, it is the same as the rabbit ball of the present invention. The live attenuated vaccine suspending agent has better compounding effect, and has good safety and immune effect when applied to rabbits.

More preferably, the raw materials for the preparation of the live attenuated rabbit coccidiosis vaccine include sporulated oocysts of the precocious strains of Eimeria, Eimeria medium and Eimeria enterica.

Preferably, the live attenuated rabbit coccidiosis vaccine is prepared by separating, purifying, and sporulating coccidial oocysts.

As a preferred solution, the preparation process of the live attenuated rabbit coccidiosis vaccine is as follows: the precocious strains of Eimeria large, Eimeria medium and Eimeria intestinalis are prepared through coccidial eggs. The sacs are separated and purified, sporulated and mixed in a certain proportion.

Preferably, in the rabbit coccidiosis live attenuated vaccine, the precocious attenuated strain of Eimeria maxima has 100-3000 oocysts per head, and the precocious attenuated strain of Eimeria enterica is 500-5000 oocysts per head. Oocysts/head, the precocious attenuated strain of Eimeria medium-sized 500-6000 oocysts/head.

Preferably, in the suspension of the live attenuated rabbit coccidiosis vaccine, the consumption of the live attenuated rabbit coccidiosis vaccine is 50-80ml suspension/head vaccine (immunized by 6h, without causing stress in the rabbit population) Under the circumstance, the immunization time can be adjusted appropriately, and the corresponding water intake also needs to be adjusted).

For example, there are 2000 rabbits in a rabbit house in a farm (assuming that the rabbit is 30 days old, the water intake of each rabbit is about 10ml/h), and the water intake is about 120L according to the 6h immunization. After adding the suspending agent of the present invention according to the drinking water amount, add 2000 vaccines and stir evenly in the suspension.

In a third aspect, the present invention provides a live attenuated rabbit coccidiosis vaccine product that can be ingested orally, comprising the live attenuated rabbit coccidiosis vaccine suspension and a pharmaceutically acceptable adjuvant.

Since the young rabbits are generally fed by breast milk, the method of immunizing the young rabbits by drinking water has limitations, and the suspending agent of the present invention can make the oocysts evenly dispersed in the system, so it can not only be used for drinking water immunity but also can be used for spraying immune solution. Prevention and immunization of coccidiosis in rabbits of different ages. Since the modern large-scale rabbit farming industry has some differences in the actual time of slaughtering and weaning, it is recommended that if the rabbit group adopts the active pipeline drinking water for drinking water immunity, it is not possible to use the pipeline drinking water for spray immunization.

In the fourth aspect, the present invention provides the suspension of the live attenuated rabbit coccidiosis vaccine or the suspension of the live attenuated rabbit coccidiosis vaccine or the orally ingested rabbit coccidiosis attenuated vaccine. Application of live virus vaccine products in the breeding of rabbits.

Those skilled in the art can combine the preferred solutions to obtain preferred embodiments of the present invention.

(3) Beneficial effects

The live attenuated rabbit coccidiosis vaccine suspending agent in the present invention can realize the immunization of rabbits by drinking water. In addition, the suspending agent has a fast dissolving speed at normal temperature, so that the rabbit coccidiosis oocysts can be uniformly dispersed in water. The system is maintained for more than 6 hours without sinking, which ensures the uniformity of the immunization of the live rabbit coccidiosis vaccine, and provides strong technical support for the promotion and application of the live attenuated rabbit coccidiosis vaccine in actual production.

Description of drawings

Fig. 1 is the test result of oocyst distribution uniformity of the live attenuated rabbit coccidiosis vaccine in Test Example 1; wherein, Figs a to f correspond to the results of Examples 1 to 3 and Comparative Examples 1 to 3 in turn;

Fig. 2 is the oocyst yield statistics result and the weight gain result after the rabbit coccidiosis live attenuated vaccine immunization in Test Example 3; wherein, the left picture is the oocyst yield statistics result, and the right picture is the weight gain result;

Figure 3 shows the statistical results of oocyst yield before and after challenge with live attenuated rabbit coccidiosis vaccine in Test Example 4; the left picture is the oocyst yield before challenge; the right picture is the egg after challenge capsule yield;

Figure 4 shows the immune uniformity results of the live attenuated rabbit coccidiosis vaccine in Test Example 4 before and after the challenge; the left picture shows the immune uniformity before the challenge; the right picture shows the immune uniformity after the challenge sex;

Figure 5 shows the results of body weight changes before and after challenge with the live attenuated rabbit coccidiosis vaccine in Test Example 4; the left picture shows the body weight changes before the challenge; the right picture shows the body weight changes after the challenge ;

Figure 6 shows the results of feed conversion ratio before and after challenge with the live attenuated rabbit coccidiosis vaccine in Test Example 4; the left picture is the feed conversion ratio result before the challenge; the right picture is the feed after the challenge conversion rate results;

Figure 7 shows the statistical results of the oocyst yield before and after the challenge with the live attenuated rabbit coccidiosis vaccine in Test Example 5; the left picture is the oocyst yield before the challenge; the right picture is the egg after the challenge capsule yield;

Figure 8 shows the results of body weight changes before and after challenge with the live attenuated rabbit coccidiosis vaccine in Test Example 5; the left picture shows the body weight changes before the challenge; the right picture shows the body weight changes after the challenge .

Detailed ways

The following examples are intended to illustrate the present invention, but not to limit the scope of the present invention.

In the following embodiments, the suspending agent raw materials used are all food grade, obtained through commercial channels.

The preparation process of the used rabbit coccidiosis live attenuated vaccine is as follows: Eimeria large, Eimeria medium, and Eimeria intestinalis precocious lines are separated and purified by coccidial oocysts, and sporulated. 150 oocysts/head for the early-maturing attenuated strain of Eimeria large, 800 oocysts/head for the early-maturing attenuated strain of Eimeria enterica, and 3000 eggs for the medium-sized attenuated strain of Eimeria The capsule/head portion ratio is mixed.

Wherein, the described Eimeria large, Eimeria medium, and Eimeria intestinalis precocious lines are consistent with the corresponding coccidia used in the specific embodiments of CN110694058A.

The experimental animals were 30-day-old rabbits, purchased from Zhuolu Guangyi Rabbit Cooperative, Zhangjiakou City, Hebei Province, and raised in an isolator free of coccidial contamination.

If no specific technique or condition is indicated in the examples, the technique or condition described in the literature in the field or the product specification is used. The reagents or instruments used without the manufacturer's indication are conventional products that can be purchased through regular channels.

Example 1

The present embodiment provides a suspending agent for a live attenuated rabbit coccidiosis vaccine, which contains a suspending compound with a mass fraction of 0.2‰ and KCl with a mass fraction of 1%, and the suspending compound has a mass ratio of 1: 1. Composition of pectin and xanthan gum.

The preparation method is as follows: respectively taking pectin, xanthan gum and KCl in parts by mass, and mixing with water, stirring and evenly mixing.

Example 2

The present embodiment provides a suspension preparation of a live attenuated rabbit coccidiosis vaccine, which is different from the embodiment 1 in that the mass fraction of KCl is 0.8%.

Example 3

The present embodiment provides a suspension of live attenuated rabbit coccidiosis vaccine, which is different from embodiment 1 in that it does not contain KCl.

Comparative Example 1

This comparative example provides a suspending agent for a rabbit coccidiosis live attenuated vaccine, which is different from Example 1 in that it does not contain pectin and KCl.

Comparative Example 2

This comparative example provides a suspending agent for a live attenuated rabbit coccidiosis vaccine, which is different from Example 1 in that it does not contain xanthan gum and KCl.

Comparative Example 3

This comparative example provides a live attenuated rabbit coccidiosis vaccine suspending agent, which is different from Example 1 in that pectin and xanthan gum are replaced with the same amount of carboxymethyl cellulose.

Test Example 1 Suspending agent maintains the suspension of vaccine oocysts in vaccine solution

Test procedure: Take 1000ml of the suspending agent in Examples 1-3 and Comparative Examples 1-3, put it into a 2L beaker, and use a pipette to take the live attenuated rabbit coccidiosis vaccine (4 million oocysts) and add it to the beaker , after fully stirring, use a pipette at 1000-8000, 6000-4000, 2000-0ml of the beaker at 0, 2, 4, 6, and 8 hours after the prepared coccidioid vaccine mixture (ie The upper, middle and lower layers of the vaccine suspension) respectively pipet 100 μL of the coccidial vaccine mixture into a clean 1.5 ml centrifuge tube, add 900 μL of saturated saline to each centrifuge tube, and then pipette and mix with a 1 ml pipette to fill the liquid. Put them into a McMaster counting plate, count the number of oocysts under a 10x microscope, and take 10 samples from each layer. Taking 0 hours as the base, the suspending effect of the oocysts after standing for different times was calculated and counted, and the results are shown in Table 1.

Table 1. Oocyst distribution uniformity test results

The results (see Fig. 1; wherein, the figures a to f correspond to the results of Examples 1 to 3 and Comparative Examples 1 to 3 in turn) show that within 6 hours of Examples 1 to 3 of the present invention, the ratio of the number of oocysts is within the range Inside, the vaccine oocyst neither floats nor sinks, which meets the suspension requirements, and the addition of KCl will not affect the suspension stability of the vaccine suspension. However, the dissolution time of the suspending agent in Example 3 is much longer than that in Example 1 and Example 2. In contrast, the suspension stability of Comparative Examples 1, 2, and 3 was poor and did not meet the suspension requirements.

Test Example 2 Speed test of suspending agent dissolved in water

In the modern intensive rabbit breeding industry, the breeding scale of rabbit farms is generally large, and the work efficiency in any link can have an impact on the benefits of the breeding farm. It must be noted that in the large-scale rabbit raising industry, there are as many as 2,000 rabbits in each rabbit house. The corresponding rabbits drink a lot of water and add too much suspending agent, which will waste a lot of time in dissolving the suspending agent. , which affects the application of live attenuated rabbit coccidiosis vaccines in actual production. Therefore, the suspending agent of the live coccidial oocyst vaccine must dissolve rapidly in the process of using the rabbit coccidial vaccine to meet the requirements of high-efficiency modern rabbit production.

In this experimental example, the dissolution rates of the suspending agents of Examples 1 to 3 were tested respectively, and the specific process was as follows: according to the proportions in Examples 1 to 3, the raw materials were respectively weighed, and 1000 ml of water was added at the same stirring speed. In a 2L beaker, let stand and record the dissolution time, the results are shown in Table 2 below.

Table 2

The results show that adding KCl to the suspending agent can speed up the dissolution rate, and it can be dissolved immediately when the concentration of KCl is 1%.

Test example 3 Toxicity test of suspending agent on rabbit coccidioid sporozoites and on rabbit body itself

Rabbit coccidia oocysts have thick oocyst walls, which can resist strong external pressure and harmful effects. But when the oocyst enters the rabbit, the sporozoites in the oocyst are released by the mechanical action of the stomach and the action of digestive enzymes in the gut. The sporozoites are very fragile after losing the protection of the oocyst wall and the sporocyst wall, and may be in direct contact with the suspending agent ingested by the rabbit body. Therefore, it is very necessary to verify whether the suspending agent component is toxic to the rabbit coccidioid sporozoites in the vaccine component.

Test procedure: 30-day-old rabbits were randomly divided into four groups according to their body weight. The first group of 3 rabbits was not inoculated as a blank control group; the second group of 3 rabbits was orally vaccinated; the third group of 3 rabbits were given water for 6 hours. Immunization: In the fourth group, 3 rabbits were vaccinated by drinking water for 24 hours, and the rabbits in the four groups were isolated and raised under the same conditions, and were vaccinated after a 5-day adaptation period. The water consumption of rabbits around 30 days old was monitored under the condition of laboratory isolator as shown in Table 3:

Table 3. Water consumption of rabbits

That is, the water intake of each rabbit about 30 days old is about 10ml/h. The water consumption of rabbits varies greatly with the influence of air temperature, feed intake and other conditions. This data is measured when the temperature is 26 degrees Celsius under the condition of the isolator, the ventilation is good, and the food intake is normal.

Groups 3 and 4 were prepared into suspensions of corresponding concentrations according to Example 1 of the present invention, and 181 ml and 726 ml of the suspensions were added to 3 vaccines for 6-hour and 24-hour drinking water immunization, respectively. The incubation periods of the precocious strains were: E.magna 96h (4d), E.media72h (3d), E.intestinal132h (5.5d). According to the incubation period of the precocious strains, the feces from 3-14d after immunization were collected to count the oocysts to determine The effective inoculation dose, and the rabbits were weighed before inoculation and 14 days after inoculation to determine the weight gain after immunization. The immunization method is as follows in Table 4:

Table 4. Immunization modalities

Figure 2 shows the statistical results of oocyst production and weight gain after immunization with live attenuated rabbit coccidiosis vaccine; the left figure is the statistical result of oocyst production, and the right figure is the weight gain result. The results show that the addition of the suspending agent in the present invention will not affect the yield of oocysts, that is, the suspending agent will not have a toxic effect on the sporozoites; the weight gain of the rabbit has no obvious change, that is, the intake of the suspending agent will not affect the rabbits. Toxic effects on the body. Therefore, the use of suspending agent for drinking water immunization of live attenuated rabbit coccidiosis vaccine will not affect the immune effect of the vaccine and the growth and health of rabbits.

Test Example 4 Immunization by drinking water

In order to explore the application effect of the vaccine suspending agent in actual production, the rabbits were immunized with drinking water.

Test procedure: 150 30-day-old rabbits were randomly divided into 5 groups according to their body weight. The 30 rabbits in the first group were not inoculated as a blank control group; the 30 rabbits in the second group received oral inoculation; 6 hours of drinking water immunization (groups 3 and 4 have separate water lines, and the immunization status of rabbits at 5 locations were randomly checked.); 30 rabbits in group 4 were immunized with drinking water for 6 hours (this group did not add assistance). Suspension), 30 rabbits in group 5 were not immunized but challenged. Vaccination was performed after a 5-day acclimation period. 14 days after immunization, each rabbit was orally inoculated with 10,000 oocysts of Eimeria large, Eimeria medium, and Eimeria intestinalis wild strains.

After the third group was prepared into a suspension of the corresponding concentration according to Example 1 of the present invention, 1.8L of the suspension was added to 30 vaccines for 6h small drinking water immunization. 30 doses of the vaccine were directly added to the vaccine for 6h small drinking water immunization. The incubation period of wild strains was: E.magna 120h (5d); E.media108h (4.5d); E.intestinal204h (8.5d). According to the incubation period of the precocious strain, collect the oocysts 3-14 days after vaccination to determine the effective inoculation dose; according to the incubation period of the wild strain, collect the number of oocysts 4-14 days after the challenge to determine the protective effect of the vaccine. The feasibility of drinking water immunization was determined by comparing oocyst production, immune homogeneity, body weight change, feed conversion rate, survival rate and mortality rate after vaccination and challenge. The immunization method is as follows in Table 5:

Table 5 Drinking water immunization

The statistical results of oocyst yield before and after challenge with live attenuated rabbit coccidiosis vaccine are shown in Figure 3, the results of immune uniformity are shown in Figure 4, the results of body weight changes are shown in Figure 5, and the results of feed conversion rate are shown in Figure 6; In Figures 3-6, the left picture is the result before the challenge, and the right picture is the result after the challenge; the survival rate and the death rate are shown in Table 6. The results showed that, compared with the drinking water immunity without the suspending agent group, the drinking water immunization effect of the suspending agent group was better, and the immune uniformity was better. Since the drinking water immunization experiment was not in an isolator, but in an open environment, the blank group also had coccidial infection.

Table 6 Survival rate and mortality rate

Test Example 5 Spray immunization

Since the mother rabbit cannot be immunized with maternal antibodies after immunization, the pups also need to be immunized. Experiments have shown that the pups before the age of 20 days are not susceptible to coccidia, while the rabbits at the age of 30 days are completely Therefore, the preventive immunization of young rabbits should be between 20 and 30 days of age. Considering that the young rabbits are too young and their susceptibility will affect the immunization of the vaccine, 25-day-old rabbits were selected for immunization in this experiment.

Test procedure: 4 litters of 25-day-old young rabbits (8 in each litter), each litter is one group. Group 1 was taken as blank group, group 2 was immunized by spray, group 3 was immunized by direct oral administration, and group 4 was not immunized but only challenged. The rabbits were raised in a coccidio-free isolator and challenged 14 days after immunization. Each rabbit was orally inoculated with 10,000 oocysts of Eimeria large, Eimeria medium, and Eimeria intestinalis wild strains. The feces of the rabbits in each group were collected 3-14 days after immunization and 4-14 days after the challenge to check the oocyst production, as well as the body weight changes of the rabbits in each group during the immunization and challenge. The effect of spray immunization was assessed according to oocyst production and body weight changes. The immunization methods are shown in Table 7, wherein, spray immunization refers to adding 8 doses of vaccine to 10 ml of the auxiliary suspension, evenly mixing, and then spraying it on the surface of the litter for immunization.

Table 7 Spray Immunization

The results are shown in Figures 7 to 8, wherein Figure 7 is the statistical result of oocyst yield before and after the challenge, and Figure 8 is the weight change results before and after the challenge; in Figures 7 to 8, the left panel The results are before the challenge, and the picture on the right is the result after the challenge. The results show that spray immunization can produce a certain immune protection effect, and the method is simple to operate, and has certain advantages for young rabbits who cannot be immunized by drinking water.

Although the present invention has been described in detail above with general description, specific embodiments and tests, some modifications or improvements can be made on the basis of the present invention, which is obvious to those skilled in the art . Therefore, these modifications or improvements made without departing from the spirit of the present invention fall within the scope of the claimed protection of the present invention.

Claims (12)

1. The rabbit coccidiosis attenuated live vaccine suspending agent is characterized by comprising 0.2 per mill of suspending complex agent, 0.8-1.0% of KCl and water in mass percentage, wherein the suspending complex agent comprises the following components in percentage by mass of 1: (0.8-1.2) pectin and xanthan gum.

2. The rabbit coccidiosis attenuated live vaccine suspending agent according to claim 1, wherein the pectin, xanthan gum and KCl are all food grade.

3. A suspension of live attenuated rabbit coccidiosis vaccine comprising live attenuated rabbit coccidiosis vaccine and the suspending agent of live attenuated rabbit coccidiosis vaccine as claimed in any one of claims 1-2.

4. The rabbit coccidiosis attenuated live vaccine suspension of claim 3, wherein the rabbit coccidiosis attenuated live vaccine is prepared from sporolated oocysts of precocious strains of Eimeria macrotype, Eimeria mesotype and Eimeria intestinalis.

5. The rabbit coccidiosis attenuated live vaccine suspension according to claim 3 or 4, wherein the rabbit coccidiosis attenuated live vaccine is prepared by separation, purification and sporulation of coccidian oocysts.

6. The rabbit coccidiosis attenuated live vaccine suspension according to claim 3 or 4, wherein in the rabbit coccidiosis attenuated live vaccine, 100-3000 oocysts per head of Eimeria macrolepis strain, 500-5000 oocysts per head of Eimeria enterocolitica strain, and 500-6000 oocysts per head of Eimeria mesomicturi strain.

7. The rabbit coccidiosis attenuated live vaccine suspension as claimed in claim 5, wherein in the rabbit coccidiosis attenuated live vaccine, 100-3000 oocysts per head of Eimeria macrolepis strain, 500-5000 oocysts per head of Eimeria enterocolis strain, and 500-6000 oocysts per head of Eimeria mesomicta strain.

8. The rabbit coccidiosis attenuated live vaccine suspension according to any one of claims 3, 4 and 7, wherein the rabbit coccidiosis attenuated live vaccine is used in an amount of 50-80ml per head vaccine.

9. The rabbit coccidiosis attenuated live vaccine suspension of claim 5, wherein the rabbit coccidiosis attenuated live vaccine is administered in an amount of 50-80ml suspension per head vaccine.

10. The rabbit coccidiosis attenuated live vaccine suspension of claim 6, wherein the rabbit coccidiosis attenuated live vaccine is administered in an amount of 50-80ml suspension per head vaccine.

11. An orally ingestible live rabbit coccidiosis attenuated vaccine product comprising the live rabbit coccidiosis attenuated vaccine suspension according to any one of claims 3 to 10 and a pharmaceutically acceptable adjuvant thereof.

12. Use of the rabbit coccidiosis attenuated live vaccine suspending agent of claim 1 or 2, or the rabbit coccidiosis attenuated live vaccine suspension of any one of claims 3 to 10, or the orally ingestible rabbit coccidiosis attenuated live vaccine product of claim 11, for the preparation of a medicament for breeding rabbits.

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