CN111323362A - Circulating tumor cell detection system and method based on fluorescence section - Google Patents
Circulating tumor cell detection system and method based on fluorescence section Download PDFInfo
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Abstract
The invention discloses a circulating tumor cell detection system and a circulating tumor cell detection method based on a fluorescent section, and relates to the field of computer image recognition. The method comprises the following steps: the fluorescence scanning module is used for scanning the fluorescence slices to obtain a fluorescence fragment image of each fluorescence channel, and performing image fusion on the fluorescence fragment image to obtain a fluorescence scanning fusion image; the image processing module specifically comprises: the characteristic extraction unit is used for respectively carrying out cell characteristic extraction on each fluorescence scanning fusion image to obtain corresponding cell image characteristics; and the characteristic matching unit is used for respectively matching the cell image characteristics with the inherent characteristics of the circulating tumor cells, outputting the corresponding fluorescence scanning fusion image as a suspected image when the cell image characteristics have the inherent characteristics, and further examining the suspected image by a doctor to confirm whether the circulating tumor cells are contained in the fluorescence section. Has the following beneficial effects: the identification accuracy and efficiency of the circulating tumor cells are improved.
Description
Technical Field
The invention relates to the field of computer image recognition, in particular to a circulating tumor cell detection system and a circulating tumor cell detection method based on a fluorescent section.
Background
Circulating Tumor Cells (CTC) belong to rare cells in peripheral blood circulation and are harmful cells in the process of Tumor metastasis and recurrence. These tumor cells from the primary tumor focus invade the blood vessels (active infiltration), shed and enter the blood circulation system to become circulating tumor cells. Some circulating tumor cells become apoptotic cells (dying CTCs) as they enter the blood circulation system, some become non-metastatic circulating tumor cells (non-metastatic CTCs) as the blood continues to circulate, while other metastatic circulating tumor cells (metastatic CTCs) can adhere, infiltrate and exit the blood vessels at a specific organ, eventually forming new tumor metastases. Therefore, the early detection of the circulating tumor cells in the blood plays an important guiding role in the prognosis judgment, the curative effect evaluation and the individualized treatment of patients.
Emerging fluorescent circulating tumor cell detection techniques and diagnostics have become a research hotspot and enter clinical applications, and the general conventional flow system is as follows:
1. collecting blood, and separating rare cells of circulating rare cells by a differential phase enrichment (SE) method or an ultra-pure SE method;
2. performing a plurality of immunostaining on circulating rare cells;
3. doctors generally use a fluorescence microscope to look and traverse all slice positions under a 20X objective lens and a 10X eyepiece, continuously switch fluorescence channels, locate complete or necrotic circulating tumor cells and CK, EpCAM, HER2, Vimentin, CD133 or other proteins in subclasses thereof and non-diploid chromosomes for analysis through abnormal expression of cells, and analyze vascular endothelial cells CEC and other rare cells;
4. and (4) the doctor checks again by the method in the same step (2) to judge whether the missed diagnosis exists or not, and the misdiagnosis manual screenshot is used for obtaining a diagnosis report.
Aiming at the detection technology, doctors are required to manually switch fluorescence channels, subjective judgment factors of the doctors exist, and a plurality of doctors are required to perform recheck; manual screenshot is needed, the electronic image cannot be scanned at any time, the electronic image is inconvenient to check, and the accuracy is low; in order to solve the problems, the invention changes the defect of manually switching the fluorescence channel in the past and improves the identification efficiency of the circulating tumor cells; the problem of misjudgment and misjudgment caused by subjective factors of doctors when doctors manually identify the circulating tumor cells in the prior art is solved, the accuracy of identifying the circulating tumor cells is improved, the identification of the circulating tumor cells can be effectively controlled to be completed within the optimal observation time of fluorescent substances in a fluorescent slice, and the identification rate of the circulating tumor cells is further improved.
Disclosure of Invention
In order to solve the above problems, the present invention provides a circulating tumor cell detection system based on fluorescence slice, comprising:
the fluorescence scanning module is used for scanning pre-made fluorescence slices of a tumor patient, scanning each scanning view field of each fluorescence slice to obtain a fluorescence fragmentation graph related to each fluorescence channel of the fluorescence scanning device in the scanning process, and carrying out image fusion on the corresponding fluorescence fragmentation graphs to obtain a fluorescence scanning fusion graph corresponding to each scanning view field;
the image processing module is connected with the fluorescence scanning module, and the image processing module specifically comprises:
the characteristic extraction unit is used for respectively carrying out cell characteristic extraction on each fluorescence scanning fusion image to obtain corresponding cell image characteristics;
and the feature matching unit is connected with the feature extraction unit and is used for respectively matching the cell image features with the inherent features of the circulating tumor cells, and outputting the corresponding fluorescence scanning fusion image as a suspected image suspected of containing the circulating tumor cells when the cell image features have the inherent features, so that a doctor can further examine the suspected image to confirm whether the fluorescent section contains the circulating tumor cells.
Preferably, the fluorescence slice is provided with a white fixed frame, and a doctor places the slice to be detected of the tumor patient in the white fixed frame.
Preferably, the fluorescence scanning module comprises:
a scanning platform for placing the pre-fabricated fluorescence section of the tumor patient;
the scanning module is used for carrying out automatic focusing on the fluorescence slices so as to take a slice area contained in the white fixed frame as a scanning plane of the fluorescence slices;
a control module respectively connected with the scanning platform and the scanning module, the control module comprising:
the first control unit is used for controlling the scanning module to switch the fluorescence channels according to a preset rule in the process of scanning the current field of view according to the current field of view of the scanning plane so as to obtain and output a fluorescence broken graph of the current field of view corresponding to each fluorescence channel;
the second control unit is connected with the first control unit and used for controlling the scanning platform to act to drive the fluorescence section to act after the current view scanning is finished so as to scan the next view of the scanning plane as the current view until all views of the scanning plane are scanned;
the image storage unit is connected with the first control unit and used for storing the fluorescence fragmentation image;
and the image processing unit is connected with the image storage unit and is used for fusing the fluorescence fragmentation images corresponding to all the fields of view to obtain a fluorescence scanning fusion image after the scanning of all the fields of view of the scanning plane is finished, and sending the fluorescence scanning fusion image to the image storage unit for storage.
Preferably, the image processing system further comprises an image reading platform connected to the image processing module, and the image reading platform comprises:
the image display unit is used for receiving and displaying each suspected image so that a doctor can further check each suspected image to obtain a check result of whether the suspected image contains the circulating tumor cells;
and the report output unit is connected with the image display unit and used for generating and outputting a corresponding detection report according to the audit result.
Preferably, the fluorescent channel comprises a red fluorescent channel, and/or a green fluorescent channel, and/or a blue fluorescent channel.
A circulating tumor cell detection method based on a fluorescence slice is applied to a circulating tumor cell detection system based on the fluorescence slice, and comprises the following steps:
step S1, the circulating tumor cell detection system based on the fluorescence slice scans the fluorescence slice of the tumor patient which is prepared in advance, and in the scanning process, aiming at each scanning visual field of each fluorescence slice, the fluorescence broken image of each fluorescence channel which is related to the fluorescence scanning device is obtained by scanning, and the corresponding fluorescence broken images are subjected to image fusion to obtain the fluorescence scanning fusion image which is corresponding to each scanning visual field;
step S2, the circulating tumor cell detection system based on the fluorescence slice respectively extracts the cell characteristics of each fluorescence scanning fusion image to obtain corresponding cell image characteristics;
step S3, the circulating tumor cell detection system based on the fluorescence slice respectively matches the cell image features with intrinsic features of circulating tumor cells, and outputs the corresponding fluorescence scan fusion map as a suspected image suspected of containing the circulating tumor cells when the cell image features have the intrinsic features, so that a doctor can further review the suspected image to confirm whether the fluorescence slice contains the circulating tumor cells.
Preferably, the circulating tumor cell detection system based on the fluorescence slice comprises a scanning platform, the fluorescence slice is provided with a white fixed frame, and a doctor places the to-be-detected slice of the tumor patient in the white fixed frame.
The step S1 includes:
step S11, placing the pre-made fluorescence section of the tumor patient on the scanning platform;
step S12, the circulating tumor cell detection system based on the fluorescence slice carries out automatic focusing on the fluorescence slice so as to take the slice area contained in the white fixed frame as the scanning plane of the fluorescence slice;
step S13, the circulating tumor cell detection system based on the fluorescence slice controls the scanning module to switch fluorescence channels according to a preset rule in the process of scanning the current field of view according to the current field of view of the scanning plane, so as to obtain and output a fluorescence fragmentation image of the current field of view corresponding to each fluorescence channel;
step S14, after the current field of view is scanned, the circulating tumor cell detection system based on the fluorescence slice controls the scanning platform to move to drive the fluorescence slice to move, so that the next field of view of the scanning plane is used as the current field of view to be scanned until all fields of view of the scanning plane are scanned;
and step S15, after the circulating tumor cell detection system based on the fluorescence slice finishes scanning all the fields of view of the scanning plane, fusing all the fluorescence fragments corresponding to all the fields of view to obtain a fluorescence scanning fusion image, and storing the fluorescence scanning fusion image.
Preferably, the method further comprises an interpretation method, and the steps of the interpretation method comprise:
step A, the circulating tumor cell detection system based on the fluorescence slice receives and displays each suspected image, so that a doctor can further check each suspected image to obtain a check result of whether the suspected image contains the circulating tumor cells;
and B, generating a corresponding detection report by the circulating tumor cell detection system based on the fluorescence slice according to the auditing result and outputting the detection report.
Has the following beneficial effects:
in order to improve the identification accuracy and identification efficiency of the circulating tumor cells, the defect of manual switching of a fluorescence channel in the prior art is overcome, and the identification efficiency of the circulating tumor cells is improved; the problem of misjudgment and missed judgment caused by subjective factors of doctors when the circulating tumor cells are manually identified by the doctors in the prior art is solved, and the accuracy of identifying the circulating tumor cells is improved; meanwhile, the recognition of the circulating tumor cells can be effectively controlled within the optimal observation time of the fluorescent substance in the fluorescent slice, and the recognition rate of the circulating tumor cells is further improved.
Drawings
FIG. 1 is a schematic diagram of a system for detecting circulating tumor cells based on fluorescence sectioning according to a preferred embodiment of the present invention;
FIG. 2 is a schematic flow chart of a method for detecting circulating tumor cells based on fluorescence sectioning according to a preferred embodiment of the present invention;
FIG. 3 is a flow chart illustrating a process for obtaining a fluorescence scan fusion map according to a preferred embodiment of the present invention;
fig. 4 is a flowchart illustrating a reading method according to a preferred embodiment of the invention.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
It should be noted that the embodiments and features of the embodiments may be combined with each other without conflict.
The invention is further described with reference to the following drawings and specific examples, which are not intended to be limiting.
In order to solve the above problems, the present invention provides a circulating tumor cell detection system based on fluorescence slice, as shown in fig. 1, comprising:
the fluorescence scanning module 1 is used for scanning pre-made fluorescence slices of a tumor patient, scanning each scanning view field of each fluorescence slice to obtain a fluorescence fragmentation image related to each fluorescence channel of the fluorescence scanning device in the scanning process, and performing image fusion on the corresponding fluorescence fragmentation images to obtain a fluorescence scanning fusion image corresponding to each scanning view field;
the image processing module 2 is connected with the fluorescence scanning module 1, and the image processing module 2 specifically comprises:
the feature extraction unit 21 is configured to perform cell feature extraction on each fluorescence scanning fusion image to obtain corresponding cell image features;
and the feature matching unit 22 is connected to the feature extraction unit 21, and is configured to match the cell image features with the intrinsic features of the circulating tumor cells, and output the corresponding fluorescence scanning fusion map as a suspected image suspected of containing the circulating tumor cells when the cell image features have the intrinsic features, so that a doctor can further review the suspected image to determine whether the fluorescence slice contains the circulating tumor cells.
Specifically, in the embodiment, in order to improve the identification accuracy and the identification efficiency of the circulating tumor cells, the defect that a fluorescence channel needs to be manually switched in the past is overcome, the identification efficiency of the circulating tumor cells is improved, the fluorescent scanning fusion map is automatically identified through the circulating tumor cell detection system based on the fluorescent section, misjudgment and misjudgment caused by the existence of subjective factors of doctors when the circulating tumor cells are manually identified by the doctors in the past are solved, the identification accuracy of the circulating tumor cells is improved, the identification of the circulating tumor cells and the final judgment of the doctors can be effectively controlled within the optimal observation time of fluorescent substances in the fluorescent section, and the identification rate of the circulating tumor cells is further improved. This circulating tumor cell detecting system based on fluorescence section includes: the system comprises a fluorescence scanning module 1 and a fluorescence scanning module 1, wherein the fluorescence scanning module 1 is used for scanning a fluorescence slice, each cell tissue in a scanned image obtained by scanning needs to be clear enough to identify circulating tumor cells, in order to improve the scanning definition, the fluorescence slice is subjected to multiple block scanning to obtain a plurality of fluorescence broken pictures, preferably, the fluorescence broken pictures are obtained by driving a scanning platform for placing the fluorescence slice by a stepping motor according to a certain movement displacement, and then the obtained fluorescence broken pictures are fused to obtain a fluorescence scanning fusion picture containing all substance areas to be detected on the fluorescence slice; the image processing module 2 is configured to identify the obtained fluorescence scanning fusion image, the feature extraction unit 21 extracts a cell image feature in the fluorescence scanning fusion image, matches the identified cell image feature with an intrinsic feature of a circulating tumor cell through the feature matching unit 22, screens and obtains a suspected image suspected of containing the circulating tumor cell through the matching screening, and outputs the suspected image for further examining and verifying the suspected image to determine whether the fluorescence slice contains the circulating tumor cell, thereby completing the determination of whether the fluorescence slice contains the circulating tumor cell.
In the preferred embodiment of the invention, the fluorescence section has a white fixed frame, and the doctor places the section to be detected of the tumor patient in the white fixed frame.
Specifically, in this embodiment, after the slice to be detected is placed in the white fixed frame, the substance to be detected in the slice to be detected is conveniently searched, and the substance to be detected is completely scanned and fused to obtain the fluorescence scanning fusion map.
In a preferred embodiment of the present invention, the fluorescence scanning module 1 comprises:
a scanning platform 11 for placing a pre-made fluorescence slice of a tumor patient;
the scanning module 12 is used for automatically focusing the fluorescence slices so as to take the slice area contained in the white fixed frame as a scanning plane of the fluorescence slices;
a control module 13, respectively connected to the scanning platform 11 and the scanning module 12, the control module including:
the first control unit 131 is configured to control the scanning module to switch the fluorescence channels according to a preset rule in a process of scanning a current field of view of the scanning plane to obtain and output a fluorescence histogram of the current field of view corresponding to each fluorescence channel;
the second control unit 132 is connected to the first control unit 131, and configured to control the scanning platform to move to drive the fluorescence slice to move after the current field of view is scanned, so that the next field of view of the scanning plane is used as the current field of view to be scanned until all fields of view of the scanning plane are scanned;
an image storage unit 133 connected to the first control unit 131 for storing the fluoroscopic images;
and the image processing unit 134 is connected to the image storage unit 133, and is configured to fuse all the fluorescence fragments corresponding to all the fields of view to obtain a fluorescence scanning fusion map after the scanning of all the fields of view of the scanning plane is completed, and send the fluorescence scanning fusion map to the image storage unit for storage.
Specifically, in this embodiment, first, the prepared fluorescence slice is placed on the scanning platform 11 dedicated for placing the fluorescence slice; then, automatically focusing the fluorescence slice, wherein the focusing takes a scanning plane where a slice area contained in the white fixed frame is located as a focus to obtain the clearest image of the slice area; then, the first control unit 131 switches and scans the fluorescence channels required for detection in the current visual field to obtain and output fluorescence fragmentation images under multiple fluorescence channels required for detection; then the second control unit 132 controls the scanning platform to move to scan the next field of view, preferably, the stepping motor regularly performs precise movement to obtain the next field of view, and then the first control unit 131 performs the same method to obtain the fluorescence fragmentation image of the next field of view, and so on until all fields of view of the scanning plane are scanned to obtain a plurality of fluorescence fragmentation images; all the obtained fluoroscopic images are saved by the image storage unit 133; finally, the image processing unit 134 fuses the fluorescence fragments obtained under each fluorescence channel to obtain a fluorescence scanning fusion image.
In a preferred embodiment of the present invention, the present invention further includes a reading platform 3 connected to the image processing module 2, wherein the reading platform 3 includes:
the image display unit 31 is configured to receive and display each suspected image, so that a doctor further examines each suspected image to obtain an examination result of whether the suspected image contains circulating tumor cells;
and the report output unit 32 is connected with the image display unit 31, and is used for generating and outputting a corresponding detection report according to the audit result.
Specifically, in this embodiment, the radiographing platform 3 is mainly provided for the doctor to perform radiographing and examination, and the image display unit 31 is configured to display a suspected image including circulating tumor cells for the doctor to further review, obtain a review result according to the review, and output the review result through the report output unit 32.
In a preferred embodiment of the present invention, the fluorescent channels comprise a red fluorescent channel, and/or a green fluorescent channel, and/or a blue fluorescent channel.
Specifically, in this embodiment, the fluorescence channel includes, but is not limited to, a red fluorescence channel, and/or a green fluorescence channel, and/or a blue fluorescence channel, and different fluorescence channels are selected according to the detection requirement.
A circulating tumor cell detection method based on a fluorescence slice is applied to a circulating tumor cell detection system based on a fluorescence slice, as shown in FIG. 2, and comprises the following steps:
step S1, scanning the pre-made fluorescence slices of the tumor patient by the circulating tumor cell detection system based on the fluorescence slices, scanning each scanning field of view of each fluorescence slice to obtain a fluorescence fragmentation image related to each fluorescence channel of the fluorescence scanning device in the scanning process, and carrying out image fusion on the corresponding fluorescence fragmentation images to obtain a fluorescence scanning fusion image corresponding to each scanning field of view;
step S2, respectively carrying out cell feature extraction on each fluorescence scanning fusion image by the circulating tumor cell detection system based on the fluorescence slice to obtain corresponding cell image features;
step S3, the circulating tumor cell detection system based on the fluorescence slice matches the cell image features with the intrinsic features of the circulating tumor cells, and outputs the corresponding fluorescence scan fusion map as a suspected image suspected of containing the circulating tumor cells when the cell image features have the intrinsic features, so that a doctor can further review the suspected image to confirm whether the fluorescence slice contains the circulating tumor cells.
In a preferred embodiment of the present invention, the system for detecting circulating tumor cells based on fluorescence slices comprises a scanning platform, wherein the fluorescence slices have a white fixed frame, and a doctor places the slices to be detected of a tumor patient in the white fixed frame.
As shown in fig. 3, step S1 includes:
step S11, placing the prepared fluorescent section of the tumor patient on a scanning platform;
step S12, the circulating tumor cell detection system based on the fluorescence slice carries out automatic focusing on the fluorescence slice so as to take the slice area contained in the white fixed frame as the scanning plane of the fluorescence slice;
step S13, aiming at the current visual field of the scanning plane, the circulating tumor cell detection system based on the fluorescence slice controls the scanning module to switch the fluorescence channels according to a preset rule in the process of scanning the current visual field to obtain and output a fluorescence fragmentation image of the current visual field corresponding to each fluorescence channel;
step S14, after the scanning of the current visual field is finished, the circulating tumor cell detection system based on the fluorescence slice controls the scanning platform to act to drive the fluorescence slice to act, so that the next visual field of the scanning plane is used as the current visual field to be scanned until all the visual fields of the scanning plane are scanned;
and step S15, after the circulating tumor cell detection system based on the fluorescence slice finishes scanning all the fields of view of the scanning plane, fusing all the fluorescence fragments corresponding to all the fields of view to obtain a fluorescence scanning fusion image, and storing the fluorescence scanning fusion image.
In a preferred embodiment of the present invention, the present invention further includes a reading method, as shown in fig. 4, the reading method includes the steps of:
a, receiving and displaying each suspected image by a circulating tumor cell detection system based on a fluorescent slice so that a doctor can further examine each suspected image to obtain an examination result of whether the suspected image contains circulating tumor cells;
and B, generating a corresponding detection report by the circulating tumor cell detection system based on the fluorescence section according to the auditing result and outputting the detection report.
While the invention has been described with reference to a preferred embodiment, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the spirit and scope of the invention.
Claims (8)
1. A circulating tumor cell detection system based on fluorescence sectioning, comprising:
the fluorescence scanning module is used for scanning pre-made fluorescence slices of a tumor patient, scanning each scanning view field of each fluorescence slice to obtain a fluorescence fragmentation graph related to each fluorescence channel of the fluorescence scanning device in the scanning process, and carrying out image fusion on the corresponding fluorescence fragmentation graphs to obtain a fluorescence scanning fusion graph corresponding to each scanning view field;
the image processing module is connected with the fluorescence scanning module, and the image processing module specifically comprises:
the characteristic extraction unit is used for respectively carrying out cell characteristic extraction on each fluorescence scanning fusion image to obtain corresponding cell image characteristics;
and the feature matching unit is connected with the feature extraction unit and is used for respectively matching the cell image features with the inherent features of the circulating tumor cells, and outputting the corresponding fluorescence scanning fusion image as a suspected image suspected of containing the circulating tumor cells when the cell image features have the inherent features, so that a doctor can further examine the suspected image to confirm whether the fluorescent section contains the circulating tumor cells.
2. The system as claimed in claim 1, wherein the fluorescence section has a white frame, and the doctor places the section to be detected of the tumor patient in the white frame.
3. The fluorescence slice-based circulating tumor cell detection system of claim 2, wherein the fluorescence scanning module comprises:
a scanning platform for placing the pre-fabricated fluorescence section of the tumor patient;
the scanning module is used for carrying out automatic focusing on the fluorescence slices so as to take a slice area contained in the white fixed frame as a scanning plane of the fluorescence slices;
a control module respectively connected with the scanning platform and the scanning module, the control module comprising:
the first control unit is used for controlling the scanning module to switch the fluorescence channels according to a preset rule in the process of scanning the current field of view according to the current field of view of the scanning plane so as to obtain and output a fluorescence broken graph of the current field of view corresponding to each fluorescence channel;
the second control unit is connected with the first control unit and used for controlling the scanning platform to act to drive the fluorescence section to act after the current view scanning is finished so as to scan the next view of the scanning plane as the current view until all views of the scanning plane are scanned;
the image storage unit is connected with the first control unit and used for storing the fluorescence fragmentation image;
and the image processing unit is connected with the image storage unit and is used for fusing the fluorescence fragmentation images corresponding to all the fields of view to obtain a fluorescence scanning fusion image after the scanning of all the fields of view of the scanning plane is finished, and sending the fluorescence scanning fusion image to the image storage unit for storage.
4. The circulating tumor cell detection system based on fluorescence section as claimed in claim 1, further comprising a reading platform connected to the image processing module, the reading platform comprising:
the image display unit is used for receiving and displaying each suspected image so that a doctor can further check each suspected image to obtain a check result of whether the suspected image contains the circulating tumor cells;
and the report output unit is connected with the image display unit and used for generating and outputting a corresponding detection report according to the audit result.
5. The fluorescence slice-based circulating tumor cell detection system of claim 1, wherein the fluorescence channel comprises a red fluorescence channel, and/or a green fluorescence channel, and/or a blue fluorescence channel.
6. A circulating tumor cell detection method based on fluorescence section, which is applied to the circulating tumor cell detection system based on fluorescence section as claimed in any one of claims 1 to 5, and comprises:
step S1, the circulating tumor cell detection system based on the fluorescence slice scans the fluorescence slice of the tumor patient which is prepared in advance, and in the scanning process, aiming at each scanning visual field of each fluorescence slice, the fluorescence broken image of each fluorescence channel which is related to the fluorescence scanning device is obtained by scanning, and the corresponding fluorescence broken images are subjected to image fusion to obtain the fluorescence scanning fusion image which is corresponding to each scanning visual field;
step S2, the circulating tumor cell detection system based on the fluorescence slice respectively extracts the cell characteristics of each fluorescence scanning fusion image to obtain corresponding cell image characteristics;
step S3, the circulating tumor cell detection system based on the fluorescence slice respectively matches the cell image features with intrinsic features of circulating tumor cells, and outputs the corresponding fluorescence scan fusion map as a suspected image suspected of containing the circulating tumor cells when the cell image features have the intrinsic features, so that a doctor can further review the suspected image to confirm whether the fluorescence slice contains the circulating tumor cells.
7. The method as claimed in claim 6, wherein the system comprises a scanning platform, the fluorescence section has a white fixed frame, and the doctor places the section to be detected of the tumor patient in the white fixed frame.
The step S1 includes:
step S11, placing the pre-made fluorescence section of the tumor patient on the scanning platform;
step S12, the circulating tumor cell detection system based on the fluorescence slice carries out automatic focusing on the fluorescence slice so as to take the slice area contained in the white fixed frame as the scanning plane of the fluorescence slice;
step S13, the circulating tumor cell detection system based on the fluorescence slice controls the scanning module to switch fluorescence channels according to a preset rule in the process of scanning the current field of view according to the current field of view of the scanning plane, so as to obtain and output a fluorescence fragmentation image of the current field of view corresponding to each fluorescence channel;
step S14, after the current field of view is scanned, the circulating tumor cell detection system based on the fluorescence slice controls the scanning platform to move to drive the fluorescence slice to move, so that the next field of view of the scanning plane is used as the current field of view to be scanned until all fields of view of the scanning plane are scanned;
and step S15, after the circulating tumor cell detection system based on the fluorescence slice finishes scanning all the fields of view of the scanning plane, fusing all the fluorescence fragments corresponding to all the fields of view to obtain a fluorescence scanning fusion image, and storing the fluorescence scanning fusion image.
8. The method for circulating tumor cell detection based on fluorescence section as claimed in claim 6, further comprising a scoring method, wherein the scoring method comprises the steps of:
step A, the circulating tumor cell detection system based on the fluorescence slice receives and displays each suspected image, so that a doctor can further check each suspected image to obtain a check result of whether the suspected image contains the circulating tumor cells;
and B, generating a corresponding detection report by the circulating tumor cell detection system based on the fluorescence slice according to the auditing result and outputting the detection report.
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