CN1111735C - Colour point test method for discriminating quality of biochip - Google Patents
Colour point test method for discriminating quality of biochip Download PDFInfo
- Publication number
- CN1111735C CN1111735C CN 00110899 CN00110899A CN1111735C CN 1111735 C CN1111735 C CN 1111735C CN 00110899 CN00110899 CN 00110899 CN 00110899 A CN00110899 A CN 00110899A CN 1111735 C CN1111735 C CN 1111735C
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- sample
- color
- quality
- chips
- pigment
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- 238000000018 DNA microarray Methods 0.000 title claims description 10
- 238000010998 test method Methods 0.000 title 1
- 239000000523 sample Substances 0.000 claims abstract description 36
- 238000000034 method Methods 0.000 claims abstract description 14
- 239000000049 pigment Substances 0.000 claims abstract description 14
- 238000009826 distribution Methods 0.000 claims abstract description 12
- 239000012472 biological sample Substances 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims abstract description 6
- 238000007689 inspection Methods 0.000 claims description 9
- 108020004707 nucleic acids Proteins 0.000 claims description 4
- 102000039446 nucleic acids Human genes 0.000 claims description 4
- 150000007523 nucleic acids Chemical class 0.000 claims description 4
- 230000000052 comparative effect Effects 0.000 claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims description 3
- 150000001720 carbohydrates Chemical class 0.000 claims description 2
- 238000004040 coloring Methods 0.000 claims description 2
- 235000002864 food coloring agent Nutrition 0.000 claims description 2
- 150000002632 lipids Chemical class 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 238000001514 detection method Methods 0.000 abstract 1
- 238000009776 industrial production Methods 0.000 abstract 1
- 230000008033 biological extinction Effects 0.000 description 4
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 2
- 239000002853 nucleic acid probe Substances 0.000 description 2
- 208000028782 Hereditary disease Diseases 0.000 description 1
- 208000026350 Inborn Genetic disease Diseases 0.000 description 1
- 208000024556 Mendelian disease Diseases 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 238000002425 crystallisation Methods 0.000 description 1
- 230000008025 crystallization Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
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- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The present invention relates to a color point examination method for identifying the quality of biological chips, which is characterized in that pigments are added to liquid biological samples for manufacturing chips; then the distribution sample of color points is compiled according to the array characteristics of the samples on the chips and the set color characteristics; then the chips after the samples are arrayed are observed by a video camera; the collected image signals are compared with the distribution sample of the color points by a computer, and signals relevant to the quality of the biological chips to be examined are emitted by a detection instrument. The present invention can examine the quality in time, decrease the possibility of large loss and examine every chip, and thereby, the quality is guaranteed. The present invention has the advantage of easy automation, enhances the production efficiency and is suitable for industrial production.
Description
The present invention relates to a kind of color dot inspection technique that is used for the biochip Quality Identification.
Biological (gene) chip can be used for the hereditary disease of diagnosing human, make one of biochip method and be biological sample for example the nucleic acid samples point be placed on the medium face for example on the glass sheet.Each biochip constitutes by thousands of and even up to ten thousand biological samples, and biological sample such as nucleic acid samples are colourless transparent liquid, therefore biological sample be aligned on the chip medium face formed be colourless highdensity little point (below the 0.2mm,＞1000 points/cm
2), be difficult to identify its quality with conventional instrument.Requirement to chip quality comprises following several respects: a sample can not lack; The b sample spot puts in order and can not misplace; C sample spot size is consistent; Molecular distribution unanimity in the d sample spot; E sample spot marshalling.Whether lack for sample, whether sample spot big or small unanimity and sample spot quality problems such as marshalling whether, check by the way of observing the salt crystallization that chip sample selects at present, but this way must could be used after chip manufacturing is finished a few hours.Whether molecular distribution is consistent in order that sample spot is arranged and the sample spot, then needs to test with complicated molecular biology method.After product checks comprehensively with sampling and to test manufacture by these methods, can not guarantee the quality of the product producing in batches out.At present, the method that also can both not make comprehensive inspection in time to each chip.
The purpose of this invention is to provide a kind of color dot inspection technique that is used for the biochip Quality Identification, it can overcome the above-mentioned shortcoming of existing method.
A kind of color dot inspection technique that is used for the biochip Quality Identification, it is characterized in that at first in every kind of liquid biological sample that the coremaking sheet is used, adding pigment, arrangement characteristics on chip are worked out the color point distribution sample with the color characteristic that sets per sample again, chip behind the stock layout is observed, and the picture intelligence that collects compared by computer and color point distribution sample, send signal according to comparative result about tested biochip quality.
Advantage of the present invention is (stock layout) in time to check in the chip manufacturing process, reduces the possibility of big loss, can all test to each chip, ensures the quality of products, and is easy to robotization, enhances productivity, and is applicable to industrialization production.
Below by embodiment the present invention is described.
Make a kind of genetic chip, have 1,000 nucleic acid probe points.Prepare ten kinds of pigments before making, the extinction spike length of these pigments is between 400nm-700nm.The long difference of extinction spike between per two pigments is not less than 20nm, and every kind of pigment is all accessed three kinds of variable concentrations, constitutes 30 kinds of color characteristics altogether.With computer to these thousand sample spot random arrangement one color characteristics, and constitute a color dot distribution sample and be stored in computer, arrange a sample color characteristic table simultaneously, add the tinctorial pattern sample of appointment according to this color characteristic table respectively to 1,000 nucleic acid probe samples, the probe sample of additive color is aligned on the dieelctric sheet by design sequence with the stock layout machine.Absorb chip image with the CCD camera after finishing stock layout, this image and color point distribution sample are compared.Relatively content comprises the 2. 3. 4. 6. color distribution in the color dot etc. of the 5. arrangement specification of color dot (neat degree) that puts in order of color dot of size of color dot of shade (being decided by concentration) of the 1. color of probe points (being decided by that the extinction spike is long), according to above comparative result, whether computer can draw probe points disappearance, dislocation-free is arranged, whether meet rule (size, regularity, molecular distribution consistance) conclusion.If all indexs conform to, computer can send " qualified " signal, if any inconsistent, then can send " defective " signal.
Liquid biological sample described in the present embodiment is nucleic acid (DNA, RNA) sample, protein example, sample carbohydrate, a lipid sample or come from the sample of biosome, or the potpourri of above-mentioned sample.Described pigment is artificial coloring, natural colouring matter or artificial or natural fluorchrome, or the potpourri of multiple pigment; The extinction spike length of these pigments is at visible-range (400-700nm), the fluorescence peak wavelength of fluorchrome at visible light to the infrared light spectrum scope.Described color characteristic comprises the kind and the concentration thereof of pigment.
Claims (4)
1. color dot inspection technique that is used for the biochip Quality Identification, it is characterized in that at first in every kind of liquid biological sample that the coremaking sheet is used, adding pigment, arrangement characteristics on chip are worked out the color point distribution sample with the color characteristic that sets per sample again, chip behind the stock layout is observed, and the picture intelligence that collects compared by computer and color point distribution sample, send signal according to comparative result about tested biochip quality.
2. color dot inspection technique as claimed in claim 1 is characterized in that described liquid biological sample is the potpourri of nucleic acid samples, protein example, sample carbohydrate, lipid sample or above-mentioned sample.
3. color dot inspection technique as claimed in claim 1 is characterized in that described pigment is artificial coloring, natural colouring matter or fluorchrome, or the potpourri of multiple pigment.
4. color dot inspection technique as claimed in claim 1 is characterized in that described color characteristic comprises the kind and the concentration thereof of pigment.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00110899 CN1111735C (en) | 2000-02-19 | 2000-02-19 | Colour point test method for discriminating quality of biochip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 00110899 CN1111735C (en) | 2000-02-19 | 2000-02-19 | Colour point test method for discriminating quality of biochip |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN1281142A CN1281142A (en) | 2001-01-24 |
| CN1111735C true CN1111735C (en) | 2003-06-18 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 00110899 Expired - Fee Related CN1111735C (en) | 2000-02-19 | 2000-02-19 | Colour point test method for discriminating quality of biochip |
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| Country | Link |
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| CN (1) | CN1111735C (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1297819C (en) * | 2004-09-29 | 2007-01-31 | 南京大渊生物技术工程有限责任公司 | Biological chip quantitative detecting method |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP4009311B2 (en) * | 2005-10-28 | 2007-11-14 | 三菱レイヨン株式会社 | Method for confirming probe mounting position in nucleic acid array |
| CN100419794C (en) * | 2005-12-16 | 2008-09-17 | 北京中星微电子有限公司 | A method for detecting image problems |
| WO2014025415A2 (en) * | 2012-08-08 | 2014-02-13 | Scanadu Incorporated | Method and apparatus for performing and quantifying color changes induced by specific concentrations of biological analytes in an automatically calibrated environment |
| CN103543274B (en) * | 2013-11-07 | 2015-09-09 | 南京祥中生物科技有限公司 | A kind of visual biochip |
| CN103575894B (en) * | 2013-11-07 | 2015-08-05 | 南京祥中生物科技有限公司 | A kind of detection method of visible biological chip |
| CN103869059A (en) * | 2014-03-27 | 2014-06-18 | 上海奥普生物医药有限公司 | Sample applying method of diagnostic kit |
| CN103983793A (en) * | 2014-05-29 | 2014-08-13 | 上海理工大学 | Protein chip spotting buffer liquid containing ponceau and preparation method thereof |
-
2000
- 2000-02-19 CN CN 00110899 patent/CN1111735C/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1297819C (en) * | 2004-09-29 | 2007-01-31 | 南京大渊生物技术工程有限责任公司 | Biological chip quantitative detecting method |
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| Publication number | Publication date |
|---|---|
| CN1281142A (en) | 2001-01-24 |
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