CN1111635A - Sphingomyelin extracting and application in cosmetics and foods - Google Patents
Sphingomyelin extracting and application in cosmetics and foods Download PDFInfo
- Publication number
- CN1111635A CN1111635A CN94111318A CN94111318A CN1111635A CN 1111635 A CN1111635 A CN 1111635A CN 94111318 A CN94111318 A CN 94111318A CN 94111318 A CN94111318 A CN 94111318A CN 1111635 A CN1111635 A CN 1111635A
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- Prior art keywords
- sheath
- phosphatide
- nerve
- liposome
- extracting
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- 235000013305 food Nutrition 0.000 title claims abstract description 9
- 239000002537 cosmetic Substances 0.000 title abstract description 4
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 claims abstract description 20
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 claims abstract description 19
- CSCPPACGZOOCGX-UHFFFAOYSA-N Acetone Chemical compound CC(C)=O CSCPPACGZOOCGX-UHFFFAOYSA-N 0.000 claims abstract description 18
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 10
- 235000012000 cholesterol Nutrition 0.000 claims abstract description 10
- 210000001835 viscera Anatomy 0.000 claims abstract description 6
- 238000005406 washing Methods 0.000 claims abstract description 5
- 241001465754 Metazoa Species 0.000 claims abstract description 4
- 210000005036 nerve Anatomy 0.000 claims description 17
- 239000002502 liposome Substances 0.000 claims description 14
- PORPENFLTBBHSG-MGBGTMOVSA-N 1,2-dihexadecanoyl-sn-glycerol-3-phosphate Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP(O)(O)=O)OC(=O)CCCCCCCCCCCCCCC PORPENFLTBBHSG-MGBGTMOVSA-N 0.000 claims description 6
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 6
- 235000013599 spices Nutrition 0.000 claims description 4
- 241000270295 Serpentes Species 0.000 claims description 3
- 238000000605 extraction Methods 0.000 claims description 3
- 239000012530 fluid Substances 0.000 claims description 3
- 235000011187 glycerol Nutrition 0.000 claims description 3
- 239000004166 Lanolin Substances 0.000 claims description 2
- 235000021355 Stearic acid Nutrition 0.000 claims description 2
- 229930006000 Sucrose Natural products 0.000 claims description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 2
- 230000003544 deproteinization Effects 0.000 claims description 2
- 235000012907 honey Nutrition 0.000 claims description 2
- 229940039717 lanolin Drugs 0.000 claims description 2
- 235000019388 lanolin Nutrition 0.000 claims description 2
- 239000006210 lotion Substances 0.000 claims description 2
- 238000002156 mixing Methods 0.000 claims description 2
- QIQXTHQIDYTFRH-UHFFFAOYSA-N octadecanoic acid Chemical compound CCCCCCCCCCCCCCCCCC(O)=O QIQXTHQIDYTFRH-UHFFFAOYSA-N 0.000 claims description 2
- OQCDKBAXFALNLD-UHFFFAOYSA-N octadecanoic acid Natural products CCCCCCCC(C)CCCCCCCCC(O)=O OQCDKBAXFALNLD-UHFFFAOYSA-N 0.000 claims description 2
- 102000004169 proteins and genes Human genes 0.000 claims description 2
- 108090000623 proteins and genes Proteins 0.000 claims description 2
- 235000020712 soy bean extract Nutrition 0.000 claims description 2
- 239000008117 stearic acid Substances 0.000 claims description 2
- -1 Solsperse 2000 Substances 0.000 claims 1
- IIZPXYDJLKNOIY-JXPKJXOSSA-N 1-palmitoyl-2-arachidonoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCC(=O)OC[C@H](COP([O-])(=O)OCC[N+](C)(C)C)OC(=O)CCC\C=C/C\C=C/C\C=C/C\C=C/CCCCC IIZPXYDJLKNOIY-JXPKJXOSSA-N 0.000 abstract description 3
- 235000010445 lecithin Nutrition 0.000 abstract description 3
- 229940067606 lecithin Drugs 0.000 abstract description 3
- 239000000787 lecithin Substances 0.000 abstract description 3
- 238000000034 method Methods 0.000 abstract description 3
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 abstract description 2
- 239000000741 silica gel Substances 0.000 abstract description 2
- 229910002027 silica gel Inorganic materials 0.000 abstract description 2
- 238000004809 thin layer chromatography Methods 0.000 abstract description 2
- BHEPBYXIRTUNPN-UHFFFAOYSA-N hydridophosphorus(.) (triplet) Chemical compound [PH] BHEPBYXIRTUNPN-UHFFFAOYSA-N 0.000 abstract 1
- 230000001537 neural effect Effects 0.000 description 13
- 238000003756 stirring Methods 0.000 description 5
- 229960004756 ethanol Drugs 0.000 description 3
- 239000000706 filtrate Substances 0.000 description 3
- 150000003904 phospholipids Chemical class 0.000 description 3
- 239000002994 raw material Substances 0.000 description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 3
- 239000000654 additive Substances 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 210000004556 brain Anatomy 0.000 description 2
- 239000012043 crude product Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 210000003743 erythrocyte Anatomy 0.000 description 2
- 150000002632 lipids Chemical class 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000003960 organic solvent Substances 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- LRYZPFWEZHSTHD-HEFFAWAOSA-O 2-[[(e,2s,3r)-2-formamido-3-hydroxyoctadec-4-enoxy]-hydroxyphosphoryl]oxyethyl-trimethylazanium Chemical class CCCCCCCCCCCCC\C=C\[C@@H](O)[C@@H](NC=O)COP(O)(=O)OCC[N+](C)(C)C LRYZPFWEZHSTHD-HEFFAWAOSA-O 0.000 description 1
- 241000283690 Bos taurus Species 0.000 description 1
- YDNKGFDKKRUKPY-JHOUSYSJSA-N C16 ceramide Natural products CCCCCCCCCCCCCCCC(=O)N[C@@H](CO)[C@H](O)C=CCCCCCCCCCCCCC YDNKGFDKKRUKPY-JHOUSYSJSA-N 0.000 description 1
- 206010008570 Chloasma Diseases 0.000 description 1
- 206010021718 Induced labour Diseases 0.000 description 1
- 208000003351 Melanosis Diseases 0.000 description 1
- CRJGESKKUOMBCT-VQTJNVASSA-N N-acetylsphinganine Chemical compound CCCCCCCCCCCCCCC[C@@H](O)[C@H](CO)NC(C)=O CRJGESKKUOMBCT-VQTJNVASSA-N 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 206010039792 Seborrhoea Diseases 0.000 description 1
- 210000000709 aorta Anatomy 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 229940106189 ceramide Drugs 0.000 description 1
- ZVEQCJWYRWKARO-UHFFFAOYSA-N ceramide Natural products CCCCCCCCCCCCCCC(O)C(=O)NC(CO)C(O)C=CCCC=C(C)CCCCCCCCC ZVEQCJWYRWKARO-UHFFFAOYSA-N 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 230000006837 decompression Effects 0.000 description 1
- 229960000935 dehydrated alcohol Drugs 0.000 description 1
- 230000006866 deterioration Effects 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- XTLNYNMNUCLWEZ-UHFFFAOYSA-N ethanol;propan-2-one Chemical compound CCO.CC(C)=O XTLNYNMNUCLWEZ-UHFFFAOYSA-N 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000012065 filter cake Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000010191 image analysis Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 238000002386 leaching Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- VVGIYYKRAMHVLU-UHFFFAOYSA-N newbouldiamide Natural products CCCCCCCCCCCCCCCCCCCC(O)C(O)C(O)C(CO)NC(=O)CCCCCCCCCCCCCCCCC VVGIYYKRAMHVLU-UHFFFAOYSA-N 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 230000037312 oily skin Effects 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 210000000578 peripheral nerve Anatomy 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 239000002504 physiological saline solution Substances 0.000 description 1
- 210000002706 plastid Anatomy 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000003252 repetitive effect Effects 0.000 description 1
- 210000003497 sciatic nerve Anatomy 0.000 description 1
- 238000004062 sedimentation Methods 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 238000001291 vacuum drying Methods 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
Landscapes
- Cosmetics (AREA)
Abstract
The sphingomyelin capable of using in cosmetics and food can be obtained from the following processes: crushing the animal's viscera, extracting the cholesterol with acetone, extracting the sphingomyelin (containing lecithin and albumen) with hot alcohol, removing the lecithin by ether and washing out the albumen with acetone. Through the silica gel bilaterial thin-layer chromatography and phosphorous quantity test, the content of cholesterol is less than 3%, and moisture is less than 1%.
Description
The present invention relates to the extraction of nerve (sheath) phospholipid (Sphingomyelins) and make liposome (Liposomes) film material be used for daily cosmetics and foodstuff additive with neural (sheath) phosphatide.
Neural (sheath) phospholipid is non-glycerine phosphatide, and widely distributed in vivo, function is important, but content is lower.The report (1961) that extracts neural (sheath) phosphatide from red corpuscle was once arranged, but the techniqueflow complexity yields poorly, the cost height is only as biochemical reagents.（Hanahan D.J.，Biochem.Prep 1961;8：121）
Hanahan method of reporting main contents are: anti-freezing ox blood 10L, and 4 ℃ of following centrifuging and taking red blood corpuscle, physiological saline is centrifugal again after cleaning, and gets red blood corpuscle 3.9L.The gained red corpuscle adds 0.25 volume distilled water mixed room temperature and leaves standstill 3～5 ', add 3 volumes, 95% ethanol, stir the back room temperature and place 2h.After adding the stirring of 1 volume diethyl ether, put 4h, cross leaching filtrate and preserve (4 ℃), repeat above-mentioned steps, merging filtrate, after organic solvent is removed in the placement decompression, chromatography column separates, collecting precipitation thing behind the elutriant deepfreeze, vacuum-drying, get 2.2g nerve (sheath) phosphatide, wherein lecithin content is not more than 5%.Output/raw material is 0.022%, uses ethanol 13L, diethyl ether 5L and other medicine in the production.
The objective of the invention is to avoid above-mentioned weak point of the prior art and provide a kind of, and technology is simple, cost is low, output is high, can be widely used among makeup and the food with the extraction raw material of animal viscera as neural (sheath) phosphatide.
Purpose of the present invention can reach by following measure:
1, rationale:
A, animal viscera contain nerve (sheath) phosphatide of higher proportion, and for example: aorta 38.1%(accounts for the per-cent of lipid, down together), brain 5.6%, peripheral nerve 30.2%, lung 11.1%.
B, different lipid different solubility in different organic solvents.
The acetone ethanol ether
Cholesterol is broad molten
Kephalin is insoluble not broad
Yelkin TTS is insoluble broad
Neural (sheath) phosphatide not broad (heat) is insoluble
2, technical process,
Internal organs are pulverized, acetone extracting cholesterol, hot ethanol extracting nerve (sheath) phosphatide (containing Yelkin TTS and soluble protein), Yelkin TTS is removed in the ether extracting, washing with acetone removes Deproteinization, must can be used for nerve (sheath) phosphatide (can get cholesterol, Yelkin TTS simultaneously) of makeup and food.
As use the induced labor foetus internal organs to be raw material, can get humanized's nerve (sheath) phosphatide.
The present invention has following advantage compared to existing technology:
Now the phosphatide as the liposome coating materials is mainly Yelkin TTS and kephalin.The shortcoming of their maximums is a unstable, and deterioration by oxidation very easily under the normal temperature loses effect in air.Compare with Yelkin TTS, kephalin, neural (sheath) phosphatide not only in air, stable under the illumination, and contain matter important component ceramide between skin.So the liposome that makes with it adds in the makeup, obtains peculiar effect.In addition, because its stable and many-sided function also will come into one's own as food liposome additive.
This product is nontoxicity after testing, nonirritant, and no supersensitivity, all indexs of health meet the provisions of the relevant regulations issued by the State.
With the liposome that neural (sheath) phosphatide is made, particle carefully less, be evenly distributed, it is spherical that form mostly is, minority is thruster shape (electromicroscopic photograph image analysis, the average 124.25 ± 44.33nm of diameter, (50～300nm)).
Crowd's experimental result was good after simple nerve (sheath) the phosphatide plastid of making and nerve (sheath) phosphatide-snake oil liposome added makeup to.Addition 10
-4The time, skin is moist, comfortable.Once a day, attenuate tender, flexible, wrinkle of a week back skin reduces.The skin chloasma is thin out after January.While is not sticking, oiliness, air permeability is good.
Embodiment 1,
Pig brain homogenate 200g+ acetone 300ml stirs 4h, filters.
Filter cake adds the qdx dehydrated alcohol, and 60 ℃ water-soluble.Stir 4h, filter repetitive operation 3 times.
Merging filtrate is separated out precipitation under-15 ℃.Cold filtration, drying under reduced pressure gets crude product.
Crude product adds the qdx ether, cap seal in Erlenmeyer flask or beaker, and vibration 4h, taking precipitate repeats the ether washing once.
Ether sedimentation thing washing with acetone 2 times, lyophilize gets neural (sheath) phosphatidase 10 .8g.
Embodiment 2,
Cattle sciatic nerve homogenate 100g+ acetone 200ml stirs 4h, filters.
Following steps with embodiment 1,
Get neural (sheath) phosphatidase 12 .5g.
Gained nerve (sheath) phosphatide is through two-way thin-layer chromatography of silica gel and phosphorus quantitative testing, neural (sheath) phospholipids content 〉=96%, cholesterol≤3%, moisture content＜1%.
Embodiment 3, liposome-snake gall frost
Stearic acid 12%, Solsperse 2000 8%, lanolin 4%, glycerine 10%, spices 1%, sanitas is an amount of, and smart water adds to 100%, phosphatidic acid 1.5%, cholesterol 0.5%, neural (sheath) phosphatidase 10 .01%, snake gall 0.1%.
Phosphatidic acid, cholesterol, nerve (sheath) phosphatide, snake gall are made liposome, rest part is made lotion, then thorough mixing.
Liposome-snake gall frost is specially adapted to oily skin.
Embodiment 4, liposome oral fluid
Marine alga concentrated solution 3%, soybean extract 3%, white sugar 1%, honey 1%, spices is an amount of, and sanitas is an amount of, NaHCO
30.2%, cerebrolysin 0.1%, Vitc0.1%, neural (sheath) phosphatidase 10 .01%, phosphatidic acid 1%, smart water adds to 100%.
With cerebrolysin, VitC, neural (sheath) phosphatide, phosphatidic acid is prepared into liposome, is made into liposome oral fluid jointly with other compositions then.
This kind oral liquid activeconstituents is subjected to the phosphatide protection, has obvious brain-invigorating.
Claims (3)
1, the extraction process of a kind of nerve (sheath) phosphatide and in makeup and Application in Food, it is characterized in that animal viscera is pulverized, acetone extracting cholesterol, hot ethanol extracting nerve (sheath) phosphatide (containing Yelkin TTS and soluble protein), Yelkin TTS is removed in the ether extracting, washing with acetone removes Deproteinization, must can be used for nerve (sheath) phosphatide of makeup and food.
2, the application of nerve according to claim 1 (sheath) phosphatide in makeup, it is characterized in that phosphatidic acid, cholesterol, nerve (sheath) phosphatide, snake gall are made liposome, stearic acid, Solsperse 2000, lanolin, glycerine, spices, sanitas are made lotion, then thorough mixing liposome-snake gall frost.
3, nerve according to claim 1 (sheath) phosphatide is in Application in Food, it is characterized in that cerebrolysin, Vitc, nerve (sheath) phosphatide, phosphatidic acid are prepared into liposome, then with marine alga concentrated solution, soybean extract, white sugar, honey, spices, sanitas, NaHCO
3Be made into liposome oral fluid jointly.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN94111318A CN1111635A (en) | 1994-05-10 | 1994-05-10 | Sphingomyelin extracting and application in cosmetics and foods |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN94111318A CN1111635A (en) | 1994-05-10 | 1994-05-10 | Sphingomyelin extracting and application in cosmetics and foods |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1111635A true CN1111635A (en) | 1995-11-15 |
Family
ID=5035201
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN94111318A Pending CN1111635A (en) | 1994-05-10 | 1994-05-10 | Sphingomyelin extracting and application in cosmetics and foods |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN1111635A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0887070A1 (en) * | 1997-05-30 | 1998-12-30 | Kibun Food ChemiFA Co., Ltd. | External composition for skin comprising sphingoglycolipid |
| CN101272793B (en) * | 2005-09-22 | 2011-02-09 | 雪印乳业株式会社 | Drugs, food or feed containing sphingomyelin |
| CN1997376B (en) * | 2004-03-30 | 2011-05-11 | 雪印乳业株式会社 | Skin beauty agent |
-
1994
- 1994-05-10 CN CN94111318A patent/CN1111635A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0887070A1 (en) * | 1997-05-30 | 1998-12-30 | Kibun Food ChemiFA Co., Ltd. | External composition for skin comprising sphingoglycolipid |
| CN1997376B (en) * | 2004-03-30 | 2011-05-11 | 雪印乳业株式会社 | Skin beauty agent |
| CN101272793B (en) * | 2005-09-22 | 2011-02-09 | 雪印乳业株式会社 | Drugs, food or feed containing sphingomyelin |
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| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |