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CN111004710A - Sterile plant tissue extractor based on transgenic technology - Google Patents

Sterile plant tissue extractor based on transgenic technology Download PDF

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CN111004710A
CN111004710A CN201911361246.0A CN201911361246A CN111004710A CN 111004710 A CN111004710 A CN 111004710A CN 201911361246 A CN201911361246 A CN 201911361246A CN 111004710 A CN111004710 A CN 111004710A
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extraction kettle
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CN111004710B (en
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郭俊娥
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Luliang University
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Abstract

本发明公开了一种基于转基因技术的植物组织无菌提取器，本发明通过设置支撑移动机构，这样，支撑移动机构能够驱动组织液提取釜在组织取料机构和蒸馏水供应罐之间移动，以分别对接所述组织取料机构和蒸馏水供应罐，可以有效的实现提取的流水式操作，提高简便性，并可以简化程序，以便降低外界细菌的影响可能性，本发明的组织取料机构能够自动将植物组织取块并直接置于组织液提取釜内，有效提高对组织提取的方便性，降低采用切刀切取时产生的一系列安全问题，此外，本发明的蒸馏水供应罐能够对所述组织液提取釜提供无菌蒸馏水，可以方便对组织液提取釜进行清洁消毒以及提取液的供给。

Figure 201911361246

The invention discloses a plant tissue aseptic extractor based on transgenic technology. In the present invention, a support moving mechanism is provided, so that the support moving mechanism can drive the tissue fluid extraction kettle to move between the tissue reclaiming mechanism and the distilled water supply tank to separate the Docking the tissue reclaiming mechanism and the distilled water supply tank can effectively realize the flow-through operation of extraction, improve the simplicity, and simplify the procedure, so as to reduce the possibility of the influence of external bacteria, and the tissue reclaiming mechanism of the present invention can automatically The plant tissue is taken and directly placed in the tissue fluid extraction kettle, which effectively improves the convenience of tissue extraction and reduces a series of safety problems when cutting with a cutter. In addition, the distilled water supply tank of the present invention can extract the tissue fluid Provide sterile distilled water, which can facilitate the cleaning and disinfection of the tissue fluid extraction kettle and the supply of the extraction solution.

Figure 201911361246

Description

Sterile plant tissue extractor based on transgenic technology
Technical Field
The invention relates to an aseptic extractor, in particular to a plant tissue aseptic extractor based on a transgenic technology.
Background
At present, in order to realize the analysis and research of transgenic plants, the plant tissues need to be aseptically extracted and analyzed. At present, aseptic extraction generally utilizes the extraction knife to incise plant tissues, then places in an aseptic bottle, utilizes stirring crushing equipment and the like to crush, and utilizes the extracting solution to extract, the mode is troublesome, and when extracting, the extraction knife is in contact with the outside and the problem of errors such as manual operation is solved, the aseptic property is difficult to really guarantee, and simultaneously, when cutting the tissues, the size of tissue blocks is difficult to grasp, the operation is difficult, quick running water type operation cannot be realized, and the efficiency of experiment and extraction is influenced.
Accordingly, the present invention provides a plant tissue sterile extractor based on transgenic technology to solve the problems set forth in the background art.
Disclosure of Invention
The invention aims to provide a plant tissue sterile extractor based on a transgenic technology, so as to solve the problems in the background technology.
In order to achieve the purpose, the invention provides the following technical scheme:
a plant tissue sterile extractor based on a transgenic technology comprises a tissue fluid extraction kettle, a supporting and moving mechanism, a tissue material taking mechanism and a distilled water supply tank, and is characterized in that the tissue fluid extraction kettle is transversely movably arranged on the supporting and moving mechanism, the tissue material taking mechanism and the distilled water supply tank which are transversely arranged on a transverse frame at intervals are arranged above the tissue fluid extraction kettle, and the supporting and moving mechanism can drive the tissue fluid extraction kettle to move between the tissue material taking mechanism and the distilled water supply tank so as to be respectively butted with the tissue material taking mechanism and the distilled water supply tank; the tissue taking mechanism can automatically take plant tissues into blocks and directly arrange the plant tissues into the tissue fluid extraction kettle, and the distilled water supply tank can provide sterile distilled water for the tissue fluid extraction kettle.
Further, as preferred, tissue feeding agencies holds seat and lift actuating mechanism including getting material knife tackle spare, plant tissue, wherein, plant tissue holds the seat and fixes on horizontal frame, just be provided with on the horizontal frame with plant tissue that plant tissue held the seat holds the through-hole that the chamber communicates, plant tissue holds and is provided with on the seat and stretches into plant tissue holds the material knife tackle spare of the intracavity, get material knife tackle spare by lift actuating mechanism drives and goes up and down, just plant tissue holds and lies in be provided with the plant tissue that supplies to get the material on the seat and falls into the passageway that the tissue liquid extracted the cauldron.
Further, as preferred, be provided with the big little toper cover in vertical upper end on the plant tissue holds the seat, get material sword subassembly and include blade holder and preceding tool bit, the upper end fixed connection of blade holder to lift actuating mechanism, the bottom fixed connection of blade holder preceding tool bit, blade holder and preceding tool bit are located in the toper cover, just the lower extreme of preceding tool bit is provided with the slotted hole of indent.
Further, preferably, the slot hole is in an inverted U-shaped structure.
Further, as preferred, lift actuating mechanism includes drive seat, interior piston, push rod and guide bar, wherein, the drive seat is fixed on the horizontal frame, the sealed slip is provided with interior piston in the drive seat, the lower extreme of interior piston is connected the push rod, the upper end of interior piston is provided with stretches out the guide bar outside the drive seat.
Further, as preferred, be provided with in the tissue fluid draws the cauldron and smash and rabbling mechanism, smash and rabbling mechanism including crushing leaf and circulation flow injection mechanism, the rotatable fixing of crushing leaf the center of tissue fluid draws the cauldron, the bottom of tissue fluid draws the cauldron is provided with circulation flow injection mechanism, circulation flow injection mechanism can make the interior liquid of tissue fluid extraction cauldron spout to crushing leaf.
Further, as preferred, the top that the tissue fluid drawed the cauldron is provided with connects the material mouth, smash and rabbling mechanism include circulating pump, drain pipe, injection cover and a plurality of injector head, wherein, the circulating pump is located on the support of tissue fluid drawed the cauldron bottom, the circulating pump input is connected to through the drain pipe in the tissue fluid draws the cauldron, the circulating pump output is connected to the injection cover through the injection pipe, the injection cover is fixed the interior diapire of tissue fluid drawed the cauldron, a plurality of injector heads of intercommunication are sheathe in to the injection, and each injector head all towards crushing leaf.
Further, as preferred, support moving mechanism includes base, lead screw, sliding seat, driving motor, support fixed connection through the bearing frame on the base the lead screw, the lead screw by driving motor drive rotates, slidable is provided with the sliding seat on the lead screw, the tissue fluid draws the support of cauldron through the bottom to be fixed on the sliding seat.
Further, preferably, a sliding groove is formed in the middle of the base, and the sliding seat slides along the sliding groove.
Further, as preferred, be connected with out water control flexible pipe on the distilled water supply jar, and the bottom of organizing liquid extraction cauldron still is provided with the disinfection heater that is used for heating disinfection clean.
Compared with the prior art, the invention has the beneficial effects that:
the tissue fluid extraction kettle is provided with the supporting and moving mechanism, so that the supporting and moving mechanism can drive the tissue fluid extraction kettle to move between the tissue material taking mechanism and the distilled water supply tank so as to be respectively butted with the tissue material taking mechanism and the distilled water supply tank, the continuous-flow operation of extraction can be effectively realized, the simplicity is improved, the procedure can be simplified, and the possibility of influence of external bacteria is reduced.
Drawings
FIG. 1 is a schematic diagram of the overall structure of a plant tissue sterile extractor based on transgenic technology;
fig. 2 is a schematic structural diagram of a tissue taking mechanism in a plant tissue sterile extractor based on a transgenic technology.
FIG. 3 is a schematic diagram of the internal structure of a tissue fluid extraction kettle of a plant tissue sterile extractor based on transgenic technology;
Detailed Description
Referring to fig. 1 to 3, in the embodiment of the present invention, a plant tissue sterile extractor based on a transgenic technology includes a tissue fluid extraction kettle 8, a supporting and moving mechanism, a tissue material taking mechanism and a distilled water supply tank 16, wherein the tissue fluid extraction kettle 8 is transversely movably disposed on the supporting and moving mechanism, the tissue material taking mechanism and the distilled water supply tank 16 are transversely disposed above the tissue fluid extraction kettle at intervals on a transverse frame 15, and the supporting and moving mechanism can drive the tissue fluid extraction kettle 8 to move between the tissue material taking mechanism and the distilled water supply tank 16 so as to respectively butt the tissue material taking mechanism and the distilled water supply tank 16; the tissue extracting mechanism can automatically extract plant tissue blocks and directly arrange the plant tissue blocks in the tissue fluid extraction kettle 8, and the distilled water supply tank 16 can provide sterile distilled water for the tissue fluid extraction kettle 8.
In this embodiment, tissue feeding agencies holds seat 10 and lifting drive mechanism including getting material knife tackle spare 12, plant tissue, wherein, plant tissue holds seat 10 and fixes on horizontal frame 15, just be provided with on the horizontal frame with plant tissue that plant tissue held seat 10 holds the through-hole 11 of chamber 14 intercommunication, plant tissue holds and is provided with on the seat 10 and stretches into plant tissue holds getting material knife tackle spare 12 in the chamber 14, get material knife tackle spare 12 by lifting drive mechanism drive goes up and down, just plant tissue holds and lies in on the seat 10 it falls into the passageway that tissue liquid extracted cauldron 8 to be provided with the plant tissue that supplies to get the material under material knife tackle spare 12.
The plant tissue accommodating seat 10 is provided with a vertical conical sleeve 21 with a large upper end and a small lower end, the material taking cutter assembly 12 comprises a cutter holder 22 and a front cutter head 24, the upper end of the cutter holder 22 is fixedly connected to the lifting driving mechanism, the bottom of the cutter holder is fixedly connected with the front cutter head 24, the cutter holder 22 and the front cutter head 24 are located in the conical sleeve 21, the lower end of the front cutter head 24 is provided with an inwards concave groove hole 23, and the groove hole 23 is of an inverted U-shaped structure.
The lifting driving mechanism comprises a driving seat 17, an inner piston 19, a push rod 20 and a guide rod 18, wherein the driving seat is fixed on the transverse frame, the inner piston is arranged in the driving seat in a sealing and sliding mode, the lower end of the inner piston is connected with the push rod, and the guide rod extending out of the driving seat is arranged at the upper end of the inner piston.
The tissue fluid extraction kettle 8 is internally provided with a crushing and stirring mechanism, the crushing and stirring mechanism comprises a crushing blade 7 and a circulating flow injection mechanism, the crushing blade is rotatably fixed at the center of the tissue fluid extraction kettle 8, the bottom of the tissue fluid extraction kettle 8 is provided with the circulating flow injection mechanism, and the circulating flow injection mechanism can enable liquid in the tissue fluid extraction kettle 8 to be injected to the crushing blade 7.
Tissue fluid draws cauldron 8's top is provided with and connects the material mouth, smash and rabbling mechanism include circulating pump 6, drain pipe 27, injection pipe 26, spray cover 25 and a plurality of injector head 9, wherein, the circulating pump is located tissue fluid draws on the support 4 of cauldron 8 bottom, the circulating pump input is connected to through the drain pipe in the tissue fluid draws cauldron 8, the circulating pump output is connected to through injection pipe 26 and sprays cover 25, it fixes to spray the cover tissue fluid draws cauldron 8's inner diapire, a plurality of injector heads 9 of intercommunication are sheathe in to the injection, and each injector head 9 is all towards crushing leaf 7.
The supporting and moving mechanism comprises a base 1, a screw rod 3, a sliding seat 2 and a driving motor 5, the base is fixedly connected with the screw rod through a bearing seat, the screw rod is driven to rotate by the driving motor, the sliding seat is slidably arranged on the screw rod, and a tissue fluid extraction kettle 8 is fixed on the sliding seat through a support 4 at the bottom. The middle part of base is provided with the sliding tray, the sliding seat along the sliding tray slides.
In the invention, a distilled water supply tank 16 is connected with a water outlet control extension tube 1, and the bottom of the tissue fluid extraction kettle 8 is also provided with a disinfection heater for heating, disinfection and cleaning.
The tissue fluid extraction kettle is provided with the supporting and moving mechanism, so that the supporting and moving mechanism can drive the tissue fluid extraction kettle to move between the tissue material taking mechanism and the distilled water supply tank so as to be respectively butted with the tissue material taking mechanism and the distilled water supply tank, the continuous-flow operation of extraction can be effectively realized, the simplicity is improved, the procedure can be simplified, and the possibility of influence of external bacteria is reduced.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention are equivalent to or changed within the technical scope of the present invention.

Claims (10)

1.一种基于转基因技术的植物组织无菌提取器，包括组织液提取釜(8)、支撑移动机构、组织取料机构和蒸馏水供应罐(16)，其特征在于，所述组织液提取釜(8)可横向移动的设置在所述支撑移动机构上，所述组织液提取釜的上方设置有横向间隔布置在横向架(15)上的所述组织取料机构和蒸馏水供应罐(16)，所述支撑移动机构能够驱动所述组织液提取釜(8)在所述组织取料机构和蒸馏水供应罐(16)之间移动，以便分别对接所述组织取料机构和蒸馏水供应罐(16)；所述组织取料机构能够自动将植物组织取块并直接置于所述组织液提取釜(8)内，所述蒸馏水供应罐(16)能够对所述组织液提取釜(8)提供无菌蒸馏水。1. a plant tissue aseptic extractor based on transgenic technology, comprises tissue fluid extraction still (8), support moving mechanism, tissue reclaiming mechanism and distilled water supply tank (16), it is characterized in that, described tissue fluid extraction still (8) ) can be laterally moved on the support moving mechanism, the top of the tissue fluid extraction kettle is provided with the tissue reclaiming mechanism and the distilled water supply tank (16) which are arranged at horizontal intervals on the transverse frame (15), the The supporting moving mechanism can drive the tissue fluid extraction kettle (8) to move between the tissue reclaiming mechanism and the distilled water supply tank (16), so as to dock the tissue reclaiming mechanism and the distilled water supply tank (16) respectively; the The tissue reclaiming mechanism can automatically take pieces of plant tissue and directly place them in the tissue fluid extraction kettle (8), and the distilled water supply tank (16) can provide sterile distilled water to the tissue fluid extraction kettle (8). 2.根据权利要求1所述的一种基于转基因技术的植物组织无菌提取器，其特征在于，所述组织取料机构包括取料刀组件(12)、植物组织容纳座(10)和升降驱动机构，其中，所述植物组织容纳座(10)固定在横向架(15)上，且所述横向架上设置有与所述植物组织容纳座(10)的植物组织容纳腔(14)连通的通孔(11)，所述植物组织容纳座(10)上设置有伸入所述植物组织容纳腔(14)内的取料刀组件(12)，所述取料刀组件(12)由所述升降驱动机构驱动升降，且所述植物组织容纳座(10)上位于所述取料刀组件(12)的正下方设置有供取料的植物组织落入组织液提取釜(8)内的通道。2. a kind of plant tissue aseptic extractor based on transgenic technology according to claim 1, is characterized in that, described tissue reclaiming mechanism comprises reclaiming knife assembly (12), plant tissue accommodating seat (10) and lift A driving mechanism, wherein the plant tissue accommodating seat (10) is fixed on a transverse frame (15), and the transverse frame is provided with a plant tissue accommodating cavity (14) communicating with the plant tissue accommodating seat (10) The through hole (11) of the plant tissue accommodating seat (10) is provided with a reclaiming knife assembly (12) extending into the plant tissue accommodating cavity (14), and the reclaiming knife assembly (12) consists of The lifting and lowering drive mechanism drives the lifting and lowering, and the plant tissue accommodating seat (10) is located directly below the reclaiming knife assembly (12). aisle. 3.根据权利要求2所述的一种基于转基因技术的植物组织无菌提取器，其特征在于，所述植物组织容纳座(10)上设置有竖直的上端大下端小的锥形套(21)，所述取料刀组件(12)包括刀座(22)和前刀头(24)，所述刀座(22)的上端固定连接至所述升降驱动机构，所述刀座的底部固定连接所述前刀头(24)，所述刀座(22)和前刀头(24)位于所述锥形套(21)内，且所述前刀头(24)的下端设置有内凹的槽孔(23)。3. a kind of plant tissue aseptic extractor based on transgenic technology according to claim 2, is characterized in that, described plant tissue holding seat (10) is provided with vertical upper end large lower end small conical sleeve ( 21), the reclaiming knife assembly (12) comprises a knife seat (22) and a front knife head (24), the upper end of the knife seat (22) is fixedly connected to the lift drive mechanism, and the bottom of the knife seat The front cutter head (24) is fixedly connected, the cutter seat (22) and the front cutter head (24) are located in the tapered sleeve (21), and the lower end of the front cutter head (24) is provided with an inner Recessed slot hole (23). 4.根据权利要求2所述的一种基于转基因技术的植物组织无菌提取器，其特征在于，所述槽孔(23)为倒U字型结构。4. The aseptic plant tissue extractor based on transgenic technology according to claim 2, wherein the slot (23) is an inverted U-shaped structure. 5.根据权利要求2所述的一种基于转基因技术的植物组织无菌提取器，其特征在于，所述升降驱动机构包括驱动座(17)、内活塞(19)、推杆(20)和导向杆(18)，其中，所述驱动座固定在所述横向架上，所述驱动座内密封滑动设置有内活塞，所述内活塞的下端连接所述推杆，所述内活塞的上端设置有伸出所述驱动座外的导向杆。5. a kind of plant tissue aseptic extractor based on transgenic technology according to claim 2, is characterized in that, described lift drive mechanism comprises drive seat (17), inner piston (19), push rod (20) and A guide rod (18), wherein the driving seat is fixed on the transverse frame, an inner piston is provided in the driving seat in a sealing and sliding manner, the lower end of the inner piston is connected to the push rod, and the upper end of the inner piston is A guide rod extending out of the drive seat is provided. 6.根据权利要求2所述的一种基于转基因技术的植物组织无菌提取器，其特征在于，所述组织液提取釜(8)内设置有粉碎与搅拌机构，所述粉碎与搅拌机构包括粉碎叶(7)和循环流动喷射机构，所述粉碎叶可转动的固定在所述组织液提取釜(8)的中心，所述组织液提取釜(8)的底部设置有循环流动喷射机构，所述循环流动喷射机构能够使得组织液提取釜(8)内的液体喷向所述粉碎叶(7)。6. a kind of plant tissue aseptic extractor based on transgenic technology according to claim 2, is characterized in that, described tissue fluid extraction kettle (8) is provided with pulverizing and stirring mechanism, and described pulverizing and stirring mechanism comprises pulverizing Leaf (7) and a circulating flow jet mechanism, the crushing leaves are rotatably fixed in the center of the tissue fluid extraction kettle (8), and a circulating flow jet mechanism is arranged at the bottom of the tissue fluid extraction kettle (8). The flow spray mechanism can make the liquid in the tissue fluid extraction kettle (8) spray toward the crushing blade (7). 7.根据权利要求6所述的一种基于转基因技术的植物组织无菌提取器，其特征在于，所述组织液提取釜(8)的顶部设置有接料口，所述粉碎与搅拌机构包括循环泵(6)、出液管(27)、喷射管(26)、喷射套(25)和多个喷射头(9)，其中，所述循环泵位于所述组织液提取釜(8)底部的支架(4)上，所述循环泵输入端通过出液管连接至所述组织液提取釜(8)内，所述循环泵输出端通过喷射管(26)连接至喷射套(25)，所述喷射套固定在所述组织液提取釜(8)的内底壁，所述喷射套上连通多个喷射头(9)，且各个喷射头(9)均朝向粉碎叶(7)。7. a kind of plant tissue aseptic extractor based on transgenic technology according to claim 6, is characterized in that, the top of described tissue fluid extraction kettle (8) is provided with receiving port, and described pulverizing and stirring mechanism comprises circulation A pump (6), a liquid outlet pipe (27), a jetting pipe (26), a jetting sleeve (25) and a plurality of jetting heads (9), wherein the circulating pump is located on a bracket at the bottom of the tissue fluid extraction kettle (8) (4) Above, the input end of the circulating pump is connected to the tissue fluid extraction kettle (8) through a liquid outlet pipe, and the output end of the circulating pump is connected to the spray sleeve (25) through a spray pipe (26), and the spray The sleeve is fixed on the inner bottom wall of the tissue fluid extraction kettle (8), and a plurality of ejection heads (9) are communicated with the ejection sleeve, and each ejection head (9) faces the crushing blade (7). 8.根据权利要求2所述的一种基于转基因技术的植物组织无菌提取器，其特征在于，所述支撑移动机构包括底座(1)、丝杆(3)、滑动座(2)、驱动电机(5)，所述底座上通过轴承座支撑固定连接所述丝杆，所述丝杆由所述驱动电机驱动转动，所述丝杆上可滑动的设置有滑动座，所述组织液提取釜(8)通过底部的支架(4)固定在所述滑动座上。8. a kind of plant tissue aseptic extractor based on transgenic technology according to claim 2, is characterized in that, described support moving mechanism comprises base (1), screw rod (3), sliding seat (2), drive A motor (5), the base is supported and fixedly connected to the screw rod, the screw rod is driven and rotated by the driving motor, a sliding seat is slidably provided on the screw rod, and the tissue fluid extraction kettle (8) is fixed on the sliding seat through the bracket (4) at the bottom. 9.根据权利要求8所述的一种基于转基因技术的植物组织无菌提取器，其特征在于，所述底座的中部设置有滑动槽，所述滑动座沿着所述滑动槽滑动。9 . The aseptic plant tissue extractor based on transgenic technology according to claim 8 , wherein a sliding groove is provided in the middle of the base, and the sliding seat slides along the sliding groove. 10 . 10.根据权利要求1所述的一种基于转基因技术的植物组织无菌提取器，其特征在于，蒸馏水供应罐(16)上连接有出水控制伸缩管(1)，且所述组织液提取釜(8)的底部还设置有用于加热消毒清洁的消毒加热器。10. a kind of plant tissue aseptic extractor based on transgenic technology according to claim 1, is characterized in that, on distilled water supply tank (16), is connected with water outlet control telescopic tube (1), and described tissue fluid extraction kettle ( 8) The bottom is also provided with a sterilizing heater for heating, sterilizing and cleaning.
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