CN110384725A - A kind of preparation of Chinese traditional medicine composition and its activity extract for adjusting oral cavity flora - Google Patents

Abstract

The invention relates to the technical field of traditional Chinese medicine oral care, and specifically discloses a preparation method of a traditional Chinese medicine combination for adjusting oral flora and an active extract thereof. The traditional Chinese medicine combination includes 20-30 parts of Jiangxiangru, 20-25 parts of Radix Astragali, 10-20 parts of Hangchrysanthemum, and 5-10 parts of Honeysuckle in terms of parts by mass. The present invention selects the medicinal and edible dual-purpose Chinese herbal medicines Jiangxiang, Hangchrysanthemum, and honeysuckle, and is compatible with Astragalus as a Chinese medicine composition for preventing and treating oral odor, treating oral ulcers or alleviating periodontitis, and according to the dialectical theory of etiology in traditional Chinese medicine, Jiangxiang Diuresis reduces swelling, harmonizes dampness, and reduces inflammation; Astragalus diuresis reduces swelling, detoxifies and promotes muscle growth; Chrysanthemum clears heat, detoxifies and antibacterial; Honeysuckle dispels wind-heat, clears blood toxins, and its aroma can expel evil spirits. The ingredients of various flavors work together to prevent and adjust the oral flora through compatibility. Using the traditional Chinese medicine combination or traditional Chinese medicine compound can effectively prevent and treat oral diseases caused by oral bacteria imbalance or certain pathogenic bacteria, and the oral care effect is remarkable.

Description

Preparation of a traditional Chinese medicine combination for adjusting oral flora and its active extract

technical field

The invention belongs to the technical field of traditional Chinese medicine oral care, and in particular relates to a traditional Chinese medicine combination for adjusting oral flora and preparation of active extracts thereof.

Background technique

Many people feel that they or the other party have a bad breath, which creates a certain barrier and distance between the two parties in communication, which has a great impact on life and work, and hinders normal social communication. Microorganisms colonized in the oral cavity coexist, compete and antagonize in different parts, and are closely related to the health and disease of the oral cavity of the host. In the oral cavity, the oral cavity can maintain a healthy state only if a dynamic ecological balance is maintained between the microorganisms and between the microorganisms and the oral cavity of the host. If there are foreign organisms in the oral cavity or the proportion of individual or several microorganisms inherent in the oral microecology is significantly increased and becomes the dominant bacteria in the colonization site, the balance of the oral microecological system will be broken, and flora imbalance will occur, leading to oral diseases. The distribution and total number of oral flora in patients with bad breath are significantly different from those in normal people. The number of pathogenic bacteria related to oral diseases is significantly higher than that in normal people. The pathogenic dominant bacteria decompose the substances in the oral cavity and produce volatile sulfide and Fatty acids, volatile sulfides in abnormal breath increased significantly, and bad breath appeared.

For the bad breath caused by the imbalance of oral microecology, the current solution is to solve the imbalance of bacterial flora. It is common to treat oral diseases, eliminate the production of harmful gases in the mouth, adjust diet structure, improve hygiene habits, and treat diseases with antibiotics, while using chewing gum and other substances, strengthening oral hygiene, brushing teeth every day, and using oral rinses and other measures also have certain effects of. However, the use of antibiotics is now limited, and many types of mouthwashes often have single or poor effects. The existing oral care products with traditional Chinese medicine ingredients have the function of masking oral odor, but do not have the effect of adjusting oral microorganisms, or have a certain killing effect on oral harmful bacteria, but cannot provide the effect of refreshing the mouth.

Oral ulcer is a common oral disease. As the pace of life and study becomes faster, many people tend to be in a state of tension and mental stress; or due to bad living habits, oral ulcers, a "civilized disease", occur from time to time. Oral ulcers have been associated with Colds are as common. It usually occurs on the lips, tongue, or other parts of the mouth, and it recurs frequently, with good and bad days, in both men and women. The flora in the oral cavity is symbiotic with the host. Generally, it is in a state of balance. If this balanced oral flora is destroyed, oral diseases including oral ulcers will appear. Streptococcus, Veillonella and Changes in the content of these three bacteria will directly affect the oral microecological balance.

Contents of the invention

The technical problem to be solved by the present invention is: aiming at the defects and deficiencies of the existing products that adjust the oral flora to prevent and treat oral odor, treat oral ulcers or relieve periodontitis, the effect is not satisfactory, and the effect is not satisfactory. A traditional Chinese medicine combination for adjusting oral flora and a preparation method of its active extract. In the present invention, the Chinese herbal medicines of both medicine and food, chrysanthemum, honeysuckle, and chrysanthemum, are selected for compatibility with Astragalus as a Chinese medicine composition for preventing and treating bad breath, and Chinese medicines are combined and compatible according to the dialectical theory of etiology of Chinese medicine and the law of compatibility of Chinese medicines, so as to strengthen the oral cavity. Oral care effect, through the compatibility theory of monarch, minister and assistant, all kinds of raw materials work together to prevent and adjust the oral flora, so as to improve the oral condition. Using the traditional Chinese medicine combination or traditional Chinese medicine compound can effectively prevent and treat oral diseases such as oral ulcers and periodontal diseases caused by oral bacterial imbalance or certain pathogenic bacteria, and the oral care effect is remarkable.

The present invention adopts the following technical solutions to achieve the purpose of the invention.

Firstly, the invention discloses a traditional Chinese medicine combination for adjusting oral flora, which is a direct mixture or active extract of traditional Chinese medicine raw materials.

The traditional Chinese medicine combination comprises, in terms of parts by mass: 20-30 parts of Jiangxiangru, 20-25 parts of Radix Astragali, 10-20 parts of Hangchrysanthemum, and 5-10 parts of Honeysuckle.

Lemongrass is a Lamiaceae plant, MoslachinensisMax-imak or Jiangxiangru M.chinensis- jiangxiangru. dry aboveground parts. It was first recorded in "Bielu of Famous Doctors" and listed as a middle-grade product. It has a pungent and slightly warm taste, and has the effects of sweating and relieving the surface, diuresis and swelling, and neutralizing dampness. As the authentic medicinal material of Rhizoma Rhizoma, Rhizoma Rhizoma has great clinical medicinal value. Modern research shows that Rhizoma Rhizoma contains a variety of chemical components, which have pharmacological effects such as antipyretic and anti-inflammatory. Jiangxiangzi is an important component of this traditional Chinese medicine composition. Jiangxiangzi contains aromatic substances. This aromatic substance not only has a direct killing effect on bacteria, anaerobic bacteria, viruses and acid-producing enzymes in the mouth, but also inhibits bacteria and viruses. , anaerobic bacteria and acid-producing enzyme activities, in line with the current theory of adjusting the micro-ecological balance of dental plaque (Zhou Xuedong, Hu Tao, Li Jiyao. New concept of ecological prevention and control of dental plaque [J]. Foreign Medicine. Dental Medicine Volume, 1997 (06):323-326; Hu Tao. Dental plaque microecology and its significance in oral diseases [J]. Foreign Medicine. Stomatology Volume, 1996(02): 104-105.), and has a lot of Good oral hygiene care.

Astragalus, also known as Wangsun, Baiyao cotton, and arrow Qi, is the dried root of the leguminous plant Astragalus or Astragalus in Inner Mongolia. Sweet in nature and slightly warm in flavor, it has the effects of invigorating the middle and replenishing qi, antiperspirant, diuresis and detumescence, detoxification and muscle regeneration. Astragalus is widely used clinically in traditional Chinese medicine.

Chrysanthemum chrysanthemum, pungent, sweet, bitter, slightly cold, disperses wind-heat, calms the liver and improves eyesight, clears away heat and detoxifies, and is effective against bacteria such as E. It has complete inhibitory effect, and also has some inhibitory effect on human tuberculosis.

Honeysuckle, sweet and cold in nature and fragrant, sweet and cold to clear away heat without hurting the stomach, and the fragrance is thorough and can dispel pathogens. Honeysuckle can not only disperse wind-heat, but also clear blood toxin. It is used for various febrile diseases, such as body heat, rash, spots, heat-toxin sores, sore throat, etc., and the effect is remarkable.

In the present invention, Chinese herbal medicines of both medicinal and edible uses, chrysanthemum chrysanthemum, chrysanthemum, and honeysuckle, are compatible with astragalus as a traditional Chinese medicine composition for adjusting oral flora and preventing oral odor. , is the monarch drug; Astragalus diuresis, detumescence, detoxification and muscle growth, is a drug that assists the monarch drug Jiangxiangru to strengthen the treatment of the main disease and the main syndrome, and it is a drug that has a therapeutic effect on concurrent diseases or syndromes. For example, it can improve the symptoms caused by The problem of excessive alveolar bone absorption caused by inflammation; Hangju clears heat, detoxifies and antibacterial, assists the monarch drug Jiangxiangru, ministerial drug Astragalus to strengthen the therapeutic effect and directly treat the secondary symptoms; honeysuckle dispels wind-heat, clears and relieves blood The poison and aroma are penetrating and can dispel pathogens. It is a medicine that introduces classic medicines in this prescription, and all the medicines in the prescription can directly reach the disease, and can reconcile all medicines to make them work together to dispel pathogens. Through compatibility, all kinds of raw materials play a role in preventing and adjusting the oral flora, thereby improving the oral condition. Using the traditional Chinese medicine combination or traditional Chinese medicine compound can effectively prevent and treat oral diseases such as oral ulcers and periodontal diseases caused by oral bacterial imbalance or certain pathogenic bacteria, and the oral care effect is remarkable.

Further, the adjustment of oral flora is: eliminating oral odor caused by harmful bacteria in the oral cavity, preventing oral ulcers or relieving periodontitis.

Further, the direct mixture of the traditional Chinese medicine combination is used to prepare medical preparations for adjusting oral flora, preventing and treating oral odor, preventing and treating oral ulcers or alleviating periodontitis.

Further, the active extract of the traditional Chinese medicine combination is used to prepare medical preparations, medical supplies, dietary products, and daily chemical products for adjusting oral flora and improving oral hygiene.

Furthermore, the medical preparations include powders, powders, granules, capsules and the like.

Furthermore, the medical supplies include mouthwash, chewable tablets, lozenges, effervescent tablets, popping candy, etc.

Furthermore, the dietary products include beverages, biscuits, yogurt, jelly and the like.

Furthermore, the daily chemical products include medicated toothpaste and the like.

Secondly, the invention also discloses a preparation method of the active extract of traditional Chinese medicine combination for adjusting oral flora.

The preparation method comprises the following steps: S1, pulverizing each Chinese medicine raw material in the Chinese medicine combination; S2, uniformly mixing the pulverized Chinese medicine raw materials according to the ratio of the Chinese medicine combination; S3, soaking the mixed Chinese medicine raw materials in water; S4, Chinese medicine The raw materials are decocted; S5, filtering to obtain the decoction filtrate; S6, concentrating the decoction filtrate; S7, sterilizing, subpackaging, and storing for later use.

Further, the time for soaking in water in step S3 is 0.5-2 h; the decoction of the traditional Chinese medicine raw materials in step S4 is 2-3 times, each time for 0.5-1 h; the concentration in step S6 is under the condition of vacuum<-0.08MPa Concentrate under reduced pressure.

Beneficial effect:

(1) According to the dialectical theory of etiology in traditional Chinese medicine, the present invention can reduce swelling, harmonize dampness, and reduce inflammation in Jiangxiang; Astragalus can reduce swelling, detoxify and promote granulation; , the fragrance is thorough and can dispel evil spirits. Jiangxiangzi is an important component of this traditional Chinese medicine composition. Jiangxiangzi contains aromatic substances. This aromatic substance not only has a direct killing effect on bacteria, anaerobic bacteria, viruses and acid-producing enzymes in the mouth, but also inhibits bacteria and viruses. , anaerobic bacteria and acid-producing enzyme activities, in line with the current theory of regulating the micro-ecological balance of dental plaque, and has a good role in oral hygiene care. The compatibility of Jiang Xiangrui with astragalus, honeysuckle and hangchrysanthemum can better clear away heat and detoxify, anti-inflammation, inhibit dental plaque and improve periodontitis, thereby slowing down the excessive and rapid absorption of alveolar bone caused by periodontitis. Through compatibility, all kinds of raw materials play a role in preventing and adjusting the oral flora, thereby improving the oral condition.

(2) The present invention continuously optimizes and screens product formulas and formula ratios by selecting highly safe medicinal and edible Chinese herbal medicines, and conducting comparative analysis through a large number of safety and drug efficacy tests; the product has undergone multiple testing and analysis And experiments, the effect of adjusting the oral flora and inhibiting bacteria is obvious, the safety is high, there is no obvious side effect, and the market promotion value is extremely high.

Description of drawings

Figure 1 is a photomicrograph of the effect of the active extract A of the traditional Chinese medicine combination on the alveolar bone of rats with periodontitis.

Detailed ways

The present invention will be further described below in conjunction with specific examples, but the present invention is not limited to the following examples. The methods are conventional methods unless otherwise specified. The raw materials can be obtained from open commercial channels unless otherwise specified.

Embodiment 1: the preparation of Chinese medicine combination active extract A

According to the following mass ratio: 25 parts of Jiangxiang, 22.5 parts of Radix Astragali, 15 parts of Hangchrysanthemum, and 7.5 parts of Honeysuckle, firstly, the raw materials of Chinese medicine (dry products) are crushed; secondly, the raw materials of Chinese medicine after crushing are mixed evenly and soaked in water 1 h; add water to the soaked Chinese medicine raw materials and decoct 3 times, the three decoction times are 1h, 0.5h, 0.5h respectively, and filter to obtain the decoction filtrate; then combine the decoction filtrates, and decoct under the condition of vacuum <-0.08MPa Concentrate under reduced pressure, and stop concentrating when the medicinal liquid contains 10 g/ml of crude drug; finally, sterilize and subpackage, store for later use, and obtain the active extract A of the traditional Chinese medicine combination.

Embodiment 2: the preparation of Chinese medicine combination active extract B

According to the following mass ratio: 30 parts of Jiangxiang, 20 parts of Radix Astragali, 20 parts of Chrysanthemum, and 5 parts of Honeysuckle, first, the raw materials of Chinese medicine (dry products) are crushed; secondly, the raw materials of Chinese medicine after crushing are mixed evenly and soaked in water 2h; add water to the soaked Chinese medicine raw materials and decoct twice, the second decoction time is 1h, 1h respectively, and filter to obtain the decoction filtrate; then combine the decoction filtrates, and carry out reduced pressure concentration under the condition of vacuum <-0.08MPa , stop concentrating when the medicinal solution contains crude drug 10 g/ml; finally sterilize, subpackage and store for later use to obtain the active extract B of the traditional Chinese medicine combination.

Embodiment 3: the preparation of active extract C of Chinese medicine combination

According to the following mass ratio: 20 parts of Jiangxiang, 25 parts of Radix Astragali, 10 parts of Chrysanthemum, and 10 parts of Honeysuckle, firstly, the raw materials of Chinese medicine (dry products) are crushed; secondly, the raw materials of Chinese medicine after crushing are mixed evenly and soaked in water 0.5 h; add water to the soaked Chinese medicine raw materials and decoct twice, the second decoction time is 1h, 0.5h respectively, and filter to obtain the decoction filtrate; then combine the decoction filtrates, and reduce them under the condition of vacuum <-0.08MPa Concentrate under pressure, and stop concentrating when the medicinal liquid contains 10 g/ml of crude drug; finally, sterilize and subpackage, store for later use, and obtain the active extract C of the traditional Chinese medicine combination.

Example 4: Bacteriostatic experiment of Chinese medicine combination active extract A on 9 kinds of standard strains

Dilute the active extract A of the Chinese medicine combination obtained in Example 1 to the required concentration with sodium chloride injection, and use the test tube double dilution method to investigate the effect of the extracts of different concentrations of compositions on 9 standard bacterial strains (golden yellow Staphylococcus, Escherichia coli, Candida albicans, Pseudomonas aeruginosa, Klebsiella pneumoniae, Fusobacterium nucleatum, Streptococcus mutans, Actinomyces viscosus, Prevotella intermedia) conditions and determine their minimum inhibitory concentrations. At the same time, metronidazole injection and stomatitis spray (Heilongjiang Tianlong Pharmaceutical Co., Ltd.) were used as controls. The results are shown in Table 1:

Table 1: Comparison of antibacterial effects on aerobic bacteria

From the experimental results in Table 1, it can be seen that the active extract A of the combination of traditional Chinese medicines has a significant antibacterial effect on both aerobic and anaerobic bacteria. Among them, the antibacterial effect on Escherichia coli, Candida albicans, and Klebsiella pneumoniae was significantly better than stomatitis spray; the antibacterial effect on Streptococcus mutans and Prevotella intermedia was significantly better than that Nitazole injection; the antibacterial effect on Staphylococcus aureus and Pseudomonas aeruginosa is basically the same as stomatitis spray; only the antibacterial effect on Fusobacterium nucleatum and Actinomyces viscosus is slightly worse than metronidrone azole injection.

Example 5: Healing Experiment of Chinese Medicine Combination Active Extract A on Experimental Oral Ulcer

Dilute the active extract A of the traditional Chinese medicine combination obtained in Example 1 to the required concentration with sodium chloride injection, and conduct experiments on the healing of experimental oral ulcers. The experimental design, process implementation and experimental results are as follows:

40 rats were randomly divided into 4 groups: model control group, Baiyunshan Tangyin Dongtai Pharmaceutical Co., Ltd. ice boron buccal tablet group (positive control group), high-dose group of this combination, and low-dose group of this combination. Each group 10 only. Rats in each group were anesthetized with ether, and fixed on the mouse board in supine position. Take a glass tube with a diameter of 4 mm; place a small cotton ball at one end to make it flush with the mouth of the tube; immerse the cotton ball end of the glass tube in a phenol solution with a mass fraction of 900 g/L, Soak the cotton ball with the drug solution; place it on the buccal mucosa of the rat and burn it for about 60 s. When the glass tube was removed, a white lesion with a diameter of 4-6 mm could be seen in this area; the next day, the rat's lips were wet, drooling, and the buccal mucosa was red and swollen, which meant that the oral ulcer model was successfully established.

The large-dose group of this combination: give this combination 1250μL/100g body weight (equivalent to 15 times the daily clinical dosage of humans), and instill it into the buccal mucosa ulcer of rats in an average of 5 times, with an interval of 2 hours.

The low-dose group of this combination: give this combination 415μL/100g body weight (equivalent to 5 times the daily clinical dosage of humans), and instill it into the buccal mucosa ulcer of rats in 2 times on average, with an interval of 4 hours.

Bingboron buccal tablet group: give Bingboron buccal tablet powder 1.5 mg/g, and put it on the buccal mucosa ulcer of rats in 5 times on average, with an interval of 2 hours.

The model control group received no administration.

For the above-mentioned high-dose group, low-dose group, and ice boron buccal tablet group, food and water were fasted for 30 min after each administration, and the administration was continued for 6 days. The healing of oral ulcers in rats was observed before administration every day, and the diameter of oral ulcers (expressed in mm) and the healing time and number of oral ulcers in rats were recorded 6 days after administration. Healing criteria: Ulcer diameter <1 mm was considered healed, and ulcer diameter ≥1 mm was considered non-healed. The results are shown in Table 2. Compared with the model control group, after continuous administration for 6 days, the diameter of the oral ulcer surface of rats in the large and small dose groups of this combination decreased, and the number of rats with oral ulcer healing increased, and the differences were statistically significant (P<0.01 or P<0.05 ), the results are shown in Table 2:

Table 2 Comparison of diameters of oral ulcers in rats in each group x ± s

^※ Compared with the model group, the difference is significant (P<0.05), ^﹟ Compared with the positive control group, the difference is significant (P<0.05)

From the experimental results in Table 2, it can be seen that after the successful establishment of the experimental rat oral ulcer model and the application of drugs, the active extract A of the Chinese medicine combination and the positive drug group both reduced the ulcer area, and the difference was significant (P<0.05). On the 6th day, the high dose of the Chinese medicine combination group reached a very significant difference (P<0.01), which was significantly different from the positive drug (P<0.05), indicating that the Chinese medicine combination can significantly promote the healing of experimental oral ulcers in rats.

Embodiment 6: the antibacterial test in vitro of active extract A of Chinese medicine combination

The active extract A of the Chinese medicine combination obtained in Example 1 is diluted to the required concentration with sodium chloride injection, and the method of perforating the medicine in a flat plate is used to treat Staphylococcus aureus, Escherichia coli and Candida albicans. In vitro antibacterial test. The experimental design, process implementation and experimental results are as follows:

Take 7 sterilized test tubes, number them and arrange them on the test tube rack. According to the aseptic technique, add 3 mL of 1000 g/L traditional Chinese medicine composition A to the first tube, add 3 mL of sterile normal saline to the second to fourth tubes, Then suck 3 mL of 1000 g/L traditional Chinese medicine composition A into the second tube and mix well, then suck out 3 mL and add it to the third tube and mix well, suck out 3 mL from the third tube and add it to the fourth tube, and mix well. Add 2 mg/mL norfloxacin tablet aqueous solution to the fifth tube, add 1 mg/mL ketoconazole tablet aqueous solution to the sixth tube, and add sterile normal saline to the seventh tube. Take 20 mL of beef extract soup agar medium (Sabouraud medium for Candida albicans) that is cooled to 40 ℃ after heating and melting the labeled sterilized plate, shake well, and after solidification, use a sterile puncher with a diameter of 6 mm to culture Holes were uniformly punched on the base (3-4 holes/dish), each well was added with different mass fractions of the above-prepared medicinal solution and normal saline (0.2mL/well), and then cultured Staphylococcus aureus, large intestine Escherichia and Candida albicans were evenly inoculated around each hole, incubated at 37°C for 24 hours, and the diameter of the inhibition zone was observed and measured. Repeat the above operation twice, that is, make 3 parallels for each tube, and finally take the average value of the three antibacterial zone diameters. The results are shown in Table 3:

Table 3 In vitro antibacterial diameter results of active extract A of traditional Chinese medicine combination (mm)

From the experimental results in Table 3, it can be seen that the active extract A of the traditional Chinese medicine combination has obvious in vitro antibacterial effects on Staphylococcus aureus, Escherichia coli and Candida albicans.

Example 7: Stimulation test of active extract A of Chinese medicine combination on rat oral mucosa

The active extract A of the Chinese medicine combination obtained in Example 1 was diluted to the required concentration with sodium chloride injection, and the irritation test was carried out to the oral mucosa of rats, and the irritation of the oral mucosa of rats was observed after repeated administration. reaction. The experimental design, process implementation and experimental results are as follows:

The 20 rats were randomly divided into two groups, the traditional Chinese medicine combination group and the normal control group, with 10 rats in each group. The traditional Chinese medicine combination group was given this traditional Chinese medicine combination 2500μL/100g body weight (equivalent to 30 times the clinical human daily dose), and it was instilled into the buccal mucosa of rats on both sides in 5 times within 4 hours. The normal control group did not receive any treatment. After 24 hours of administration, the hyperemia and edema at the administration site were observed and recorded, and scored; the animals were observed for systemic adverse reactions. Daily administration and observation, a total of 7d; continue to observe 7d after drug withdrawal. The scoring criteria for mucosal hyperemia and edema are shown in Table 4 and Table 5, and the results are shown in Table 6;

Table 4 Mucosa irritation and scoring criteria

Table 5 Evaluation criteria for mucosal irritation intensity

Table 6 The average score of oral mucosal irritation after multiple administrations in each group n =10

From the experimental results in Table 6, it can be seen that the active extract A of the Chinese medicine combination has no irritation to the oral mucosa of rats, and is a safe Chinese medicine combination.

Example 8: The effect of active extract A of Chinese medicine combination on the curative effect and microbial flora of patients with oral ulcers

1. Research objects and screening principles

Taking 50 patients with oral ulcers treated from October 2018 to April 2019 as the research objects, they were randomly divided into observation group and control group, with 25 cases in each group. The control group consisted of 15 males and 10 females; aged 27-58 years, with an average age of (41.8±15.4) years; the duration of the disease was 20-47 days, with an average duration of (31.2±16.7) days. The traditional Chinese medicine combination observation group included 13 males and 12 females; aged 25-54 years, with an average age of (42.9±13.2) years; the duration of the disease was 23-50 days, with an average duration of (32.1±18.2) days. There was no significant difference in the general data of the two groups of patients (P>0.05), which were comparable. (1) All patients meet the diagnostic criteria for oral ulcers in "Oral Mucosal Diseases"; (2) The TCM diagnosis meets the oral ulcers in the "Guiding Principles for Clinical Research of New Chinese Medicines" issued by the Ministry of Health; (3) Have not used it in the past 6 months Traditional Chinese medicine; (4) Those who have not used hormone drugs in the past 6 months; (5) Obtained the informed consent of the patients and their families.

2 treatment methods

2.1 Control group

Apply levamisole liniment (Fuzhou Chenxing Pharmaceutical Co., Ltd., Zhunzi H20003344) every other day, 500 mg/time, for 2 consecutive weeks; vitamin C tablets (Northeast Pharmaceutical Group Shenyang No. 1 Pharmaceutical Co., Ltd., Zhunzi H21022862) Oral, 0.2g/time, 3 times a day. Vitamin B 12 (Shanghai Xinyi Jiufu Pharmaceutical Co., Ltd., Zhunzi H31022370) was taken orally, 10mg/time, 3 times a day.

2.2 Observation group

Use a combination of traditional Chinese medicines for treatment, dry the extract, grind it into powder and put it into a plastic vial with a nozzle, spray the ulcer wound lightly, 3 times a day, and do not drink or eat for at least 1 hour after spraying the medicine.

The curative effect was evaluated after 14 days of treatment in both groups.

3 Treatment Outcomes

3.1 Criteria for judging curative effect

Healed: all clinical symptoms disappeared; markedly effective: the ulcer wound healed and the pain disappeared; effective: the ulcer wound was reduced by ≥ 1/2, and the pain was not obvious; ineffective: the symptoms did not improve as before or the ulcer area reduced by < 1/3, Still can't eat well.

3.2 Observation indicators

The curative effect of the two groups was evaluated before and after treatment, and the duration of ulcer was counted. Before and after treatment, the number of oral Streptococcus, Neisseria and Veillonella in the two groups of patients was determined by PCR fluorescence technique.

3.3 Results

3.3.1 Comparison of curative effect between the two groups: see Table 7.

Table 7 Comparison of curative effects between the two groups (cases)

*P < 0.05 compared with the control group.

3.3.2 Comparison of ulcer duration between the two groups: see Table 8.

Table 8 Comparison of ulcer duration between the two groups (d, x ± s)

*P < 0.05 compared with the control group.

3.3.3 Comparison of oral microbial flora between the two groups: see Table 9.

Table 9 Comparison of oral microbial flora between the two groups (number, x ± s)

*Compared with this group before treatment, P<0.05; #Compared with the control group after treatment, P<0.05.

From the experimental observation of the crowd, it can be seen that the active extract A of the traditional Chinese medicine combination has a better effect on oral ulcers, which is manifested in that the total effective effect on ulcers is significantly higher than that of the control, and shortens the healing time of ulcers, and improves the beneficial bacteria related to oral ulcers. group imbalance.

Embodiment 9: The effect of Chinese medicine combination active extract A on the alveolar bone of rats with periodontitis

Select 48 5-week-old SD rats with complete tooth body and dentition, no caries and periodontal disease, half male and half female, body weight 200g±20g. They were randomly divided into normal control group, model group, Koutai group (positive treatment group, the drug used was compound chlorhexidine gargle, Jiangsu Chenpai Bangde Pharmaceutical Co., Ltd., national drug approval H20058018), Chinese medicine composition group A ( Divided into low, middle and high groups according to dose): composition A low dose group 0.5 mg/kg, composition A medium dose group 1.5 mg/kg, composition A high dose group 4.5 mg/kg, 8 rats in each group. Improve with Luo Lijun's literature method (Luo Lijun, Shu Rong, Li Chaolun, Xie Yufeng, Qiao Weiwei. Establishment of animal models of periodontitis [J]. Journal of Oral Materials and Instruments, 2005(03):120-122.). Open the mouth with a pair of large tweezers, cut and peel off the gums around the teeth on both sides with ophthalmic curved scissors, and then feed them with 10% white sugar water and soft feed soaked in white sugar water. Diet and treatment were given. Each animal was given oral instillation of each test drug at 0.1ml/100g twice a day. After the drug was fasted for 4 hours, it was fed with food and water. At the end of the experiment, the alveolar bone of the animal was taken for pathological section to observe the condition of the alveolar bone (see Figure 1).

It can be known from Figure 1 that composition A obviously inhibits the resorption of alveolar bone in experimental rats with periodontitis.

Similarly, the Chinese medicine combination active extracts B and C obtained in Examples 2 and 3 were tested according to the experimental method and experimental steps described in Examples 4-9, and the experimental results showed that: the experimental data and Examples 4-9 Similar, experimental conclusion is consistent with embodiment 4-9.

The specific embodiments of the present invention have been described in detail above, but they are only examples, and the present invention is not limited to the specific embodiments described above. For those skilled in the art, any equivalent modifications and substitutions to the present invention are also within the scope of the present invention. Therefore, equivalent changes and modifications made without departing from the spirit and scope of the present invention fall within the scope of the present invention.

Claims (8)

1. it is a kind of for adjusting the Chinese traditional medicine composition of oral cavity flora, it is the direct mixture or activity extract of Chinese medicine material, spy Sign is, the Chinese traditional medicine composition include: in terms of mass fraction 20-30 parts of HERBA ELSHOLTZIAE, 20-25 parts of Radix Astragali, 10-20 parts of Hang Ju, 5-10 parts of honeysuckle.

2. according to claim 1 for adjusting the Chinese traditional medicine composition of oral cavity flora, which is characterized in that the adjustment oral cavity Flora are as follows: oral peculiar smell caused by eliminating because of oral cavity harmful bacteria, anti-treating dental ulcer alleviate periodontitis.

3. according to claim 1 for adjusting the Chinese traditional medicine composition of oral cavity flora, it is characterised in that: the Chinese traditional medicine composition Direct mixture is used to prepare adjustment oral cavity flora, prevention and treatment oral peculiar smell, anti-treating dental ulcer or the medical system for alleviating periodontitis Agent.

4. according to claim 1 for adjusting the Chinese traditional medicine composition of oral cavity flora, it is characterised in that: the Chinese traditional medicine composition Activity extract is used to prepare medical preparation, medical articles, dietotherapy with adjustment oral cavity flora, improvement oral hygiene effect Product, daily chemical products.

5. a kind of as described in claim 1 for adjusting the preparation of the Chinese traditional medicine composition activity extract of oral cavity flora, feature exists In: the following steps are included: in S1, Chinese traditional medicine composition each Chinese medicine material crushing；S2, it is matched according to Chinese traditional medicine composition, it will be smashed Each Chinese medicine material is uniformly mixed；S3, mixed Chinese medicine material is soaked in water；S4, Chinese medicine material decoct；S5, filter pan-fried Boil filtrate；S6, the concentration for decocting filtrate；S7, sterilizing packing, store it is spare.

6. according to claim 5 for adjusting the preparation of the Chinese traditional medicine composition activity extract of oral cavity flora, feature exists In: the time soaked in water described in step S3 is 0.5-2 h.

7. according to claim 5 for adjusting the preparation of the Chinese traditional medicine composition activity extract of oral cavity flora, feature exists In: it is 2-3 times that Chinese medicine material described in step S4, which decocts, each 0.5-1h.

8. according to claim 5 for adjusting the preparation of the Chinese traditional medicine composition activity extract of oral cavity flora, feature exists In: concentration described in step S6 is to be concentrated under reduced pressure under the conditions of vacuum < -0.08MPa.