CN110326562A - Large-scale branch pin class zooplankter high-density breeding method - Google Patents
Large-scale branch pin class zooplankter high-density breeding method Download PDFInfo
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- CN110326562A CN110326562A CN201910618368.7A CN201910618368A CN110326562A CN 110326562 A CN110326562 A CN 110326562A CN 201910618368 A CN201910618368 A CN 201910618368A CN 110326562 A CN110326562 A CN 110326562A
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- 238000009395 breeding Methods 0.000 title claims abstract description 18
- 235000016709 nutrition Nutrition 0.000 claims abstract description 29
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 28
- 239000003337 fertilizer Substances 0.000 claims abstract description 24
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims abstract description 19
- 241000195493 Cryptophyta Species 0.000 claims abstract description 19
- 238000005273 aeration Methods 0.000 claims abstract description 16
- 235000008429 bread Nutrition 0.000 claims abstract description 14
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 13
- 239000001301 oxygen Substances 0.000 claims abstract description 13
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 13
- 238000005286 illumination Methods 0.000 claims abstract description 8
- 239000002994 raw material Substances 0.000 claims description 25
- 239000000523 sample Substances 0.000 claims description 23
- 210000003608 fece Anatomy 0.000 claims description 20
- 239000010871 livestock manure Substances 0.000 claims description 20
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 17
- 241000287828 Gallus gallus Species 0.000 claims description 10
- 238000009264 composting Methods 0.000 claims description 10
- 239000002054 inoculum Substances 0.000 claims description 10
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 10
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 10
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 10
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 10
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 claims description 10
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 7
- 241000894006 Bacteria Species 0.000 claims description 6
- 229920002472 Starch Polymers 0.000 claims description 5
- 238000009360 aquaculture Methods 0.000 claims description 5
- 244000144974 aquaculture Species 0.000 claims description 5
- 238000011081 inoculation Methods 0.000 claims description 5
- 235000010333 potassium nitrate Nutrition 0.000 claims description 5
- 239000004323 potassium nitrate Substances 0.000 claims description 5
- 239000000843 powder Substances 0.000 claims description 5
- 238000002360 preparation method Methods 0.000 claims description 5
- 238000004321 preservation Methods 0.000 claims description 5
- 235000010344 sodium nitrate Nutrition 0.000 claims description 5
- 239000004317 sodium nitrate Substances 0.000 claims description 5
- 235000019698 starch Nutrition 0.000 claims description 5
- 239000008107 starch Substances 0.000 claims description 5
- NUJOXMJBOLGQSY-UHFFFAOYSA-N manganese dioxide Chemical compound O=[Mn]=O NUJOXMJBOLGQSY-UHFFFAOYSA-N 0.000 claims description 4
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 3
- 241001465754 Metazoa Species 0.000 claims description 3
- 239000001110 calcium chloride Substances 0.000 claims description 3
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 3
- 239000012141 concentrate Substances 0.000 claims description 3
- SQUHHTBVTRBESD-UHFFFAOYSA-N Hexa-Ac-myo-Inositol Natural products CC(=O)OC1C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C(OC(C)=O)C1OC(C)=O SQUHHTBVTRBESD-UHFFFAOYSA-N 0.000 claims description 2
- UIIMBOGNXHQVGW-DEQYMQKBSA-M Sodium bicarbonate-14C Chemical compound [Na+].O[14C]([O-])=O UIIMBOGNXHQVGW-DEQYMQKBSA-M 0.000 claims description 2
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 2
- 235000003891 ferrous sulphate Nutrition 0.000 claims description 2
- 239000011790 ferrous sulphate Substances 0.000 claims description 2
- 239000011521 glass Substances 0.000 claims description 2
- CDAISMWEOUEBRE-GPIVLXJGSA-N inositol Chemical compound O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](O)[C@@H]1O CDAISMWEOUEBRE-GPIVLXJGSA-N 0.000 claims description 2
- 229960000367 inositol Drugs 0.000 claims description 2
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 claims description 2
- 229910000359 iron(II) sulfate Inorganic materials 0.000 claims description 2
- 238000004519 manufacturing process Methods 0.000 claims description 2
- CDAISMWEOUEBRE-UHFFFAOYSA-N scyllo-inosotol Natural products OC1C(O)C(O)C(O)C(O)C1O CDAISMWEOUEBRE-UHFFFAOYSA-N 0.000 claims description 2
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 2
- 229960001763 zinc sulfate Drugs 0.000 claims description 2
- 229910000368 zinc sulfate Inorganic materials 0.000 claims description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 claims 1
- ZAMOUSCENKQFHK-UHFFFAOYSA-N Chlorine atom Chemical compound [Cl] ZAMOUSCENKQFHK-UHFFFAOYSA-N 0.000 claims 1
- 239000011575 calcium Substances 0.000 claims 1
- 229910052791 calcium Inorganic materials 0.000 claims 1
- 239000000460 chlorine Substances 0.000 claims 1
- 229910052801 chlorine Inorganic materials 0.000 claims 1
- 238000000855 fermentation Methods 0.000 claims 1
- 230000004151 fermentation Effects 0.000 claims 1
- 238000000034 method Methods 0.000 abstract description 5
- 239000008399 tap water Substances 0.000 abstract description 5
- 235000020679 tap water Nutrition 0.000 abstract description 5
- 238000002347 injection Methods 0.000 abstract description 4
- 239000007924 injection Substances 0.000 abstract description 4
- 230000001488 breeding effect Effects 0.000 abstract description 2
- 238000003306 harvesting Methods 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 15
- 235000015097 nutrients Nutrition 0.000 description 14
- 238000005259 measurement Methods 0.000 description 9
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 6
- 230000006641 stabilisation Effects 0.000 description 6
- 238000011105 stabilization Methods 0.000 description 6
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 3
- BVKZGUZCCUSVTD-UHFFFAOYSA-N carbonic acid Chemical compound OC(O)=O BVKZGUZCCUSVTD-UHFFFAOYSA-N 0.000 description 3
- 239000001257 hydrogen Substances 0.000 description 3
- 229910052739 hydrogen Inorganic materials 0.000 description 3
- 125000004435 hydrogen atom Chemical class [H]* 0.000 description 3
- 239000011734 sodium Substances 0.000 description 3
- 229910052708 sodium Inorganic materials 0.000 description 3
- 239000007788 liquid Substances 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000012851 eutrophication Methods 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/20—Culture of aquatic animals of zooplankton, e.g. water fleas or Rotatoria
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/003—Aquaria; Terraria
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K63/00—Receptacles for live fish, e.g. aquaria; Terraria
- A01K63/04—Arrangements for treating water specially adapted to receptacles for live fish
- A01K63/042—Introducing gases into the water, e.g. aerators, air pumps
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
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- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Mycology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Animal Husbandry (AREA)
- Marine Sciences & Fisheries (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Biomedical Technology (AREA)
- Botany (AREA)
- Virology (AREA)
- Cell Biology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Farming Of Fish And Shellfish (AREA)
Abstract
The invention discloses a kind of large-scale branch pin class zooplankter high-density breeding methods, base fertilizer through everfermentation is laid in the tap water being then injected into the first culture pond Bao Qi for 24 hours, and open aeration, in second culture pond, in third culture pond, bait chlorella is cultivated respectively in 4th culture pond, flat algae and bread microzyme, corresponding nutritional agents is added after the tap water of injection aeration for 24 hours, the first culture pond dissolved oxygen is in 4mg/L ~ 6mg/L in breeding process, temperature is controlled at 25 ~ 28 DEG C, intensity of illumination is 5000lx ~ 8000lx, 8 ~ 16h of daily light application time, bait pond turbidity reaches certain numerical value Open valve and adds bait, valve is closed after being reduced to certain numerical value, first culture pond turbidity is harvested after reaching certain numerical value, stop harvesting after being reduced to certain numerical value.The present invention is able to maintain that the stable growing environment of large-scale branch pin class zooplankter, to guarantee stable acquisition large size branch pin class zooplankter.
Description
Technical field
The invention belongs to large-scale branch pin class zooplankter high-density breeding technical fields, and in particular to a kind of large size branch pin class
Zooplankter high-density breeding method.
Background technique
Large-scale branch pin class zooplankter is the important component of water ecosystem, is the secondary production in biological chain
Person plays an important role to the balance and stabilization that maintain water ecosystem.Large-scale branch pin class zooplankter can ingest rich battalion
The floating bits in blue-green alge and water body, partial suspended thing in feedingization water body etc. advantageously reduce the eutrophication of water body, improve
The transparency of water body, while branch pin class zooplankter itself is full of nutrition, can be the bait of the animals such as fish, shrimp, not have to environment
Any harm.The general individual of large-scale branch pin class zooplankter is smaller, life cycle is short, is highly prone to the shadow of external environment variation
It rings.Currently, the acquisition modes of large-scale branch pin class zooplankter are mainly field fishing and outdoor extensive culture, yield is unstable
Determine, continue the problems such as supply time is short, not can guarantee the stabilization of the quality and quantity of large-scale branch pin class zooplankter.Therefore, room
Interior high density forms major way to solve the above-mentioned problems.
Summary of the invention
In order to guarantee the stabilization of large-scale branch pin class zooplankter yield and quality, the present invention provides a kind of large-scale branch pin class
Zooplankter high-density breeding method, to guarantee the stabilization of large-scale branch pin class zooplankter yield and quality.
To achieve the goals above, the technical solution adopted by the present invention is as follows:
A kind of large size branch pin class zooplankter high-density breeding method, which comprises the following steps:
(1) base fertilizer is prepared:
The base fertilizer is prepared from the following raw materials in parts by weight: 4 ~ 6 parts of chicken manure, 5 ~ 6 parts of pig manure, 0.5 ~ 1 part of leavening, stalk 1 ~
2 parts；Wherein, chicken manure, pig manure and stalk are measured with dry weight；
The preparation step of base fertilizer specifically: weigh each raw material in parts by weight, be uniformly mixed, closed composting 40 ~ 50 days；The composting phase
Between, 60 ~ 70 DEG C are risen to central temperature, turning is primary after heat preservation 3 ~ 5 days；
(2) following three kinds of nutritional agents are configured:
The nutritional agents of chlorella is mixed according to the raw material of following parts by weight: 19 ~ 21 parts of potassium nitrate, potassium dihydrogen phosphate 7 ~ 9
Part, 0.8 ~ 1.2 part of microelement A, VB10.05 ~ 0.11 part, VB120.03 ~ 0.07 part；
The nutritional agents of flat algae is mixed according to the raw material of following parts by weight: 11 ~ 13 parts of sodium nitrate, 5 ~ 7 parts of potassium dihydrogen phosphate,
15 ~ 17 parts of sodium bicarbonate, 1 ~ 3 part of microelement A, VB10.2 ~ 0.4 part, VB120.15 ~ 0.25 part；
The nutritional agents of bread microzyme is mixed according to the raw material of following parts by weight: 9 ~ 11 parts of starch, microelement B 0.1
~ 0.3 part；
(3) cultivation apparatus is built:
The cultivation apparatus includes the first culture pond and covers in rack platform above the first culture pond, the rack platform packet
The pillar that left and right is oppositely arranged is included, is provided with horizontal platen on pillar；Is equipped on the left side strut of the rack platform
The display of one transmissometer, the first transmissometer is mounted on the left side strut of rack platform, and energy measuring temperature, and described first is turbid
The probe of degree instrument is mounted on the left side wall of the first culture pond, is equipped with dissolved oxygen meter on the first culture pond right side wall, the
The middle and lower part of one culture pond is equipped with micropore aeration pipe, light irradiation apparatus is equipped with above the first culture pond, and light irradiation apparatus is pacified
On lower plane loaded on platen, the right side wall lower portion of the first culture pond is equipped with outlet conduit, is equipped with first above pipeline
Valve；The platen upper surface has been sequentially placed the second culture pond, third culture pond and the 4th culture pond from left to right, on platen
Surface is sequentially installed with the second transmissometer, third transmissometer and the 4th transmissometer, the probe of second transmissometer from left to right
It is mounted on the left side wall of the second culture pond, the probe of the third transmissometer is mounted on the left side wall of third culture pond, institute
The probe for stating the 4th transmissometer is mounted on the left side wall of the 4th culture pond, the second culture pond, third culture pond and the 4th culture
The right side wall lower part in pond is mounted on outlet conduit, and valve is equipped on outlet conduit, and the outlet conduit is passed down through platen
And extend in the first culture pond, blender is mounted in the second culture pond, third culture pond and the 4th culture pond；
(4) by bottom in base fertilizer the first culture pond of tiling, add in the second culture pond, third culture pond and the 4th culture pond respectively
The nutritional agents for entering the nutritional agents of chlorella, the nutritional agents of flat algae and bread microzyme, to the first culture pond, the second culture pond,
It is separately added into water of the aeration after 24 hours in three culture ponds and the 4th culture pond, opens the second culture pond, third culture pond and the
Blender in four culture ponds, revolving speed are 6 ~ 8 r/s；
(5) it is inoculated with:
Acquire large-scale branch pin class animal with the zooplankters of 120 ~ 160 mesh acquisition net, be put into the first culture pond, by chlorella,
Flat algae concentrate is separately added into the second culture pond, third culture pond, and inoculum density is 300/L, addition face in the 4th culture pond
Packet yeast dry powder, inoculum concentration 5g/L；
(6) aquaculture management:
After inoculation, according to the growing state of branch pin class zooplankter large-scale in the first culture pond, valve is periodically opened, by the second training
Support pond, third culture pond, the mixed liquor in the 4th culture pond are discharged into the first culture pond；Reach to the first culture pond turbidity
20NTU collects large-scale branch pin class zooplankter, and turbidity stops collecting after being down to 5NTU in the first culture pond.
Preferably, the Dissolved Oxygen concentration Control in the first culture pond is controlled in 4mg/L ~ 6mg/L, temperature at 25 ~ 28 DEG C.
Preferably, the intensity of illumination of light irradiation apparatus is 5000lx ~ 8000lx, daily 8 ~ 16h of light application time.
Preferably, the zymophyte uses EM bacterium.
Preferably, the first culture pond, the second culture pond, third culture pond and the 4th culture pond be all made of with a thickness of 1cm ~
The glass of 2.5cm makes.
Preferably, the microelement A includes 1 part of ferrous sulfate, 2 parts of calcium chloride, 1.5 parts of zinc sulfate and manganese dioxide
0.8 part；The microelement B includes 1.6 parts of calcium chloride, 3 parts of inositol, 0.8 part of copper sulphate and 2.5 parts of Tyiamine Hd.
Large size branch pin class zooplankter high-density breeding method of the present invention, indoor culture can guarantee large-scale branch pin class
The stabilization of the growing environment of zooplankter guarantees the stabilization of yield and quality；Indoor culture can be with the growth of manual control bait
Density guarantees that large-scale branch pin class zooplankter has enough bait；The culture systems are real-time to parameters such as temperature, dissolved oxygen contents
Monitoring, is compared data, generates corresponding adjusting decision, is maintained at culture pond and is conducive to large-scale branch pin class zooplankter life
In long range；Aeration facility in culture systems can guarantee that the dissolved oxygen content in culture pond maintains large-scale branch pin class always
In the most suitable range of zooplankter growth, to guarantee the normal growth of large-scale branch pin class zooplankter.
Detailed description of the invention
Fig. 1 is the structural schematic diagram of cultivation apparatus of the present invention.
Specific embodiment
With reference to embodiment, the embodiment of the present invention is furthur described in detail.Following embodiment is used for
Illustrate the present invention, but is not intended to limit the scope of the invention.
Embodiment 1: large-scale branch pin class zooplankter high-density breeding method, its step are as follows:
(1) base fertilizer is prepared
The base fertilizer is prepared from the following raw materials in parts by weight: 4 parts of chicken manure, 5 parts of pig manure, 0.5 part of leavening, 1 part of stalk；Its
In, chicken manure, pig manure and stalk are measured with dry weight；
The preparation step of base fertilizer specifically: weigh each raw material in parts by weight, be uniformly mixed, closed composting 40 days；During composting,
60 DEG C are risen to central temperature, turning is primary after heat preservation 3 days；
(2) nutrient solution is configured
Following three kinds of nutritional agents are respectively configured:
The nutritional agents of chlorella is mixed according to the raw material of following parts by weight: potassium nitrate: 19 parts, potassium dihydrogen phosphate: 7 parts,
Microelement: 0.8 part, VB1: 0.05 part, VB12: 0.03 part；
The nutritional agents of flat algae is mixed according to the raw material of following parts by weight: 11 parts of sodium nitrate, 5 parts of potassium dihydrogen phosphate, carbonic acid
15 parts of hydrogen sodium, 1 part of microelement, VB10.2 part, VB120.15 part；
The nutritional agents of bread microzyme is mixed according to the raw material of following parts by weight: 9 parts of starch, 0.1 part of microelement；
(3) cultivation apparatus is built
As shown in Figure 1, the cultivation apparatus includes volume 4m3, the first culture pond of depth 1.2m 9 and cover on the first culture pond
The rack platform of side, the rack platform include the pillar that left and right is oppositely arranged, and are provided with horizontal platen on pillar；The branch
First transmissometer is installed, the display 11 of the first transmissometer is mounted on the left side branch of rack platform on the left side strut of body panel
On column, and energy measuring temperature, the probe 21 of first transmissometer are mounted on the left side wall of the first culture pond 9, the first culture
Dissolved oxygen meter 7 is installed, the middle and lower part of the first culture pond 9 is equipped with micropore aeration pipe 6, the first culture on 9 right side wall of pond
The top in pond 9 is equipped with light irradiation apparatus 5, and light irradiation apparatus 5 is installed on the lower plane of platen, the right side wall of the first culture pond 9
Lower portion is equipped with outlet conduit, and the first valve 41 is equipped with above pipeline；The platen upper surface is sequentially placed from left to right
There is volume 0.8m3, depth 0.6m the second culture pond 101, third culture pond 102 and the 4th culture pond 103, the second transmissometer
12, third transmissometer 13, the 4th transmissometer 14 display be installed on the second culture pond 101, third culture pond 102 and the 4th training
The left side in pond 103 is supported, each transmissometer display is connected separately with the second Turbidity measurement probe 22,23 and of third Turbidity measurement probe
4th Turbidity measurement probe 24, the turbidity meter probe is separately mounted to the second culture pond 101, third culture pond 102 and the 4th
On the left side wall of culture pond 103, the right side wall lower part of the second culture pond 101, third culture pond 102 and the 4th culture pond 103 is equal
Outlet conduit is installed, the outlet conduit is passed down through platen and extends in the first culture pond 9, the second culture pond 101,
Blender 3 is mounted in three culture ponds 102 and the 4th culture pond 103, the tap water of injection aeration for 24 hours in each culture pond, the
One culture pond depth of water 0.8m, and aeration 6 is opened, the second culture pond 101,103 depth of water of third culture pond 102 and the 4th culture pond
0.4m is separately added into configured nutrient solution, and opens blender, revolving speed 6r/s；；
(4) base fertilizer is placed in the first culture pond 9, base fertilizer tiles bottom of pond, with a thickness of 11cm, respectively in the second culture pond 103, the
The nutritional agents of 20g chlorella, the nutritional agents and 26 Saccharomyces cerevisiaes of 25 flat algaes is added in three culture ponds 102 and the 4th culture pond 101
The nutritional agents of bacterium, cultivated in the second culture pond 101, third culture pond 102 and the 4th culture pond 103 respectively chlorella, flat algae and
Bread microzyme；
(5) it is inoculated with
Large-scale bracket class is acquired with the zooplankter acquisition net of 150 mesh, is put into the first culture pond 9, purchase chlorella, flat algae are dense
The second culture pond 101, third culture pond 102 is added in contracting liquid, and inoculum density is 300/L, and bread is added in the 4th culture pond 103
Yeast dry powder, inoculum concentration 5g/L；
(6) aquaculture management
After inoculation, according to the growing state of branch pin class zooplankter large-scale in the first culture pond 9, periodically open the second valve 42,
Third valve 43 and third valve 44, by the mixed liquor in the second culture pond 101, third culture pond 102, the 4th culture pond 103
It is discharged into the first culture pond 9；Reach 20NTU to 9 turbidity of the first culture pond, collects large-scale branch pin class zooplankter, the first culture
Turbidity stops collecting after being down to 5NTU in pond；
First culture pond 9 opens the first valve 41 periodically to guarantee that the water level of the first culture pond 9 maintains 0.8m depth, opens first
When valve 41, it is cased with the string bag of 160 mesh in outlet conduit end, to prevent large-scale branch pin class zooplankter to be lost；
In 4mg/L, temperature is controlled at 25 DEG C Dissolved Oxygen concentration Control in first culture pond 9；
The intensity of illumination of the light irradiation apparatus 5 of first culture pond 9 is 5000lx, daily light application time 10h；
After culture according to the method described above, large-scale branch pin class zooplankter can capture once every 4 days.Large size can be obtained every time
Branch pin class zooplankter 200g.
Embodiment 2: large-scale branch pin class zooplankter high-density breeding method, its step are as follows:
(1) base fertilizer is prepared
The base fertilizer is prepared from the following raw materials in parts by weight: 5 parts of chicken manure, 6 parts of pig manure, 0.8 part of leavening, 1.5 parts of stalk；Its
In, chicken manure, pig manure and stalk are measured with dry weight；
The preparation step of base fertilizer specifically: weigh each raw material in parts by weight, be uniformly mixed, closed composting 45 days；During composting,
65 DEG C are risen to central temperature, turning is primary after heat preservation 4 days；
(2) nutrient solution is configured
Following three kinds of nutrient solutions are respectively configured:
The nutrient solution of chlorella is mixed according to the raw material of following parts by weight: potassium nitrate: 20 parts, potassium dihydrogen phosphate: 8 parts,
Microelement: 1 part, VB1: 0.08 part, VB12: 0.05 part；
The nutrient solution of flat algae is mixed according to the raw material of following parts by weight: 12 parts of sodium nitrate, 6 parts of potassium dihydrogen phosphate, carbonic acid
16 parts of hydrogen sodium, 2 parts of microelement, VB10.3 part, VB120.2 part；
The nutrient solution of bread microzyme is mixed according to the raw material of following parts by weight: 10 parts of starch, 0.2 part of microelement；
(3) cultivation apparatus is built
Including volume 5m3, depth 1.4m the first culture pond 9 and cover in rack platform above the first culture pond, the branch
Body panel includes the pillar that left and right is oppositely arranged, and is provided with horizontal platen on pillar；On the left side strut of the rack platform
First transmissometer is installed, the display 11 of the first transmissometer is mounted on the left side strut of rack platform, and energy measuring temperature,
The probe 21 of first transmissometer is mounted on the left side wall of the first culture pond 9, is equipped on 9 right side wall of the first culture pond molten
Oxygen analyzer 7 is solved, the middle and lower part of the first culture pond 9 is equipped with micropore aeration pipe 6, and the top of the first culture pond 9 is equipped with illumination
Equipment 5, and light irradiation apparatus 5 is installed on the lower plane of platen, the right side wall lower portion of the first culture pond 9 is equipped with outlet pipe
Road is equipped with the first valve 41 above pipeline；The platen upper surface has been sequentially placed volume 0.8m from left to right3, depth
The second culture pond 101, third culture pond 102 and the 4th culture pond 103 of 0.6m, the second transmissometer 12, third transmissometer 13,
The display of four transmissometers 14 is installed on the left side of the second culture pond 101, third culture pond 102 and the 4th culture pond 103, each turbid
Degree instrument display is connected separately with the second Turbidity measurement probe 22, third Turbidity measurement probe 23 and the 4th Turbidity measurement probe
24, the turbidity meter probe is separately mounted to the left side wall of the second culture pond 101, third culture pond 102 and the 4th culture pond 103
On, the right side wall lower part of the second culture pond 101, third culture pond 102 and the 4th culture pond 103 is mounted on outlet conduit, institute
It states outlet conduit to be passed down through platen and extend in the first culture pond 9, the second culture pond 103, third culture pond 102 and the 4th
Blender 3 is mounted in culture pond 101, the tap water of injection aeration for 24 hours in each culture pond, the first culture pond depth of water 1m, and
Aeration 6 is opened, 103 depth of water 0.4m of the second culture pond 101, third culture pond 102 and the 4th culture pond is separately added into configured
Nutrient solution, and blender is opened, revolving speed 7r/s；
(4) base fertilizer is placed in the first culture pond 9, base fertilizer tiles bottom of pond, with a thickness of 13cm, respectively in the second culture pond 103, the
The nutritional agents of 25g chlorella, the nutritional agents and 30 Saccharomyces cerevisiaes of 28 flat algaes is added in three culture ponds 102 and the 4th culture pond 101
The nutritional agents of bacterium, cultivated in the second culture pond 101, third culture pond 102 and the 4th culture pond 103 respectively chlorella, flat algae and
Bread microzyme；
(5) it is inoculated with
Large-scale bracket class is acquired with the zooplankter acquisition net of 150 mesh, is put into the first culture pond 9, purchase chlorella, flat algae are dense
The second culture pond 101, third culture pond 102 is added in contracting liquid, and inoculum density is 300/L, and bread is added in the 4th culture pond 103
Yeast dry powder, inoculum concentration 5g/L；
(6) aquaculture management
After inoculation, according to the growing state of branch pin class zooplankter large-scale in the first culture pond 9, periodically open the second valve 42,
Third valve 43 and third valve 44, by the mixed liquor in the second culture pond 101, third culture pond 102, the 4th culture pond 103
It is discharged into the first culture pond 9；Reach 20NTU to 9 turbidity of the first culture pond, collects large-scale branch pin class zooplankter, the first culture
Turbidity stops collecting after being down to 5NTU in pond；
First culture pond 9 opens the first valve 41 periodically to guarantee that the water level of the first culture pond 9 maintains 0.8m depth, opens first
When valve 41, it is cased with the string bag of 160 mesh in outlet conduit end, to prevent large-scale branch pin class zooplankter to be lost；
In 5mg/L, temperature is controlled at 27 DEG C Dissolved Oxygen concentration Control in first culture pond 9；
The intensity of illumination of the light irradiation apparatus 5 of first culture pond 9 is 6500lx, daily light application time 13h；
After culture according to the method described above, large-scale branch pin class zooplankter can capture once every 3 days.Large size can be obtained every time
Branch pin class zooplankter 240g.
Embodiment 3: large-scale branch pin class zooplankter high-density breeding method, its step are as follows:
(1) base fertilizer is prepared
The base fertilizer is prepared from the following raw materials in parts by weight: 6 parts of chicken manure, 6 parts of pig manure, 1 part of leavening, 2 parts of stalk；Wherein,
Chicken manure, pig manure and stalk are measured with dry weight；
The preparation step of base fertilizer specifically: weigh each raw material in parts by weight, be uniformly mixed, closed composting 50 days；During composting,
70 DEG C are risen to central temperature, turning is primary after heat preservation 5 days；
(2) nutrient solution is configured
Following three kinds of nutrient solutions are respectively configured:
The nutrient solution of chlorella is mixed according to the raw material of following parts by weight: potassium nitrate: 21 parts, potassium dihydrogen phosphate: 9 parts,
Microelement: 1.2 parts, VB1: 0.11 part, VB12: 0.07 part；
The nutrient solution of flat algae is mixed according to the raw material of following parts by weight: 13 parts of sodium nitrate, 7 parts of potassium dihydrogen phosphate, carbonic acid
17 parts of hydrogen sodium, 3 parts of microelement, VB1 0.4 part, VB12 0.25 part；
The nutrient solution of bread microzyme is mixed according to the raw material of following parts by weight: 11 parts of starch, 0.3 part of microelement；
(3) cultivation apparatus is built
Including volume 6m3, depth 1.5m the first culture pond 9 and cover in rack platform above the first culture pond, the branch
Body panel includes the pillar that left and right is oppositely arranged, and is provided with horizontal platen on pillar；On the left side strut of the rack platform
First transmissometer is installed, the display 11 of the first transmissometer is mounted on the left side strut of rack platform, and energy measuring temperature,
The probe 21 of first transmissometer is mounted on the left side wall of the first culture pond 9, is equipped on 9 right side wall of the first culture pond molten
Oxygen analyzer 7 is solved, the middle and lower part of the first culture pond 9 is equipped with micropore aeration pipe 6, and the top of the first culture pond 9 is equipped with illumination
Equipment 5, and light irradiation apparatus 5 is installed on the lower plane of platen, the right side wall lower portion of the first culture pond 9 is equipped with outlet pipe
Road is equipped with the first valve 41 above pipeline；The platen upper surface has been sequentially placed volume 0.8m from left to right3, depth
The second culture pond 101, third culture pond 102 and the 4th culture pond 103 of 0.6m, the second transmissometer 12, third transmissometer 13,
The display of four transmissometers 14 is installed on the left side of the second culture pond 101, third culture pond 102 and the 4th culture pond 103, each turbid
Degree instrument display is connected separately with the second Turbidity measurement probe 22, third Turbidity measurement probe 23 and the 4th Turbidity measurement probe
24, the turbidity meter probe is separately mounted on the left side wall of the second culture pond, third culture pond and the 4th culture pond, the second training
The right side wall lower part for supporting pond 101, third culture pond 102 and the 4th culture pond 103 is mounted on outlet conduit, the outlet conduit
It is passed down through platen and extends in the first culture pond 9, the second culture pond 101, third culture pond 102 and the 4th culture pond 103
It is inside mounted on blender 3, the tap water of injection aeration for 24 hours, the first culture pond depth of water 1.1m in each culture pond, and open aeration
6,103 depth of water 0.4m of the second culture pond 101, third culture pond 102 and the 4th culture pond are separately added into configured nutrient solution,
And blender is opened, revolving speed 8r/s；
(4) base fertilizer is placed in the first culture pond 9, base fertilizer tiles bottom of pond, with a thickness of 15cm, respectively in the second culture pond 101, the
The nutritional agents of 28g chlorella, the nutritional agents and 35g bread ferment of 31g flat algae is added in three culture ponds 102 and the 4th culture pond 103
The nutritional agents of female bacterium cultivates chlorella, flat algae in the second culture pond 103, third culture pond 102 and the 4th culture pond 101 respectively
And bread microzyme；
(5) large-scale bracket class is acquired with the zooplankters of 150 mesh acquisition net, be put into the first culture pond 9, it is purchase chlorella, flat
The second culture pond 101, third culture pond 102 is added in algae concentrate, and inoculum density is 300/L, is added in the 4th culture pond 103
Saccharomyces cerevisiae dry powder, inoculum concentration 5g/L；
(6) aquaculture management
After inoculation, according to the growing state of branch pin class zooplankter large-scale in the first culture pond 9, periodically open the second valve 42,
Third valve 43 and third valve 44, by the mixed liquor in the second culture pond 101, third culture pond 102, the 4th culture pond 103
It is discharged into the first culture pond 9, reaches 20NTU to 9 turbidity of the first culture pond, collect large-scale branch pin class zooplankter, the first culture
Turbidity stops collecting after being down to 5NTU in pond；
First culture pond 9 opens the first valve 41 periodically to guarantee that the water level of the first culture pond 9 maintains 0.8m depth, opens first
When valve 41, it is cased with the string bag of 160 mesh in outlet conduit end, to prevent large-scale branch pin class zooplankter to be lost；
In 6mg/L, temperature is controlled at 28 DEG C Dissolved Oxygen concentration Control in first culture pond 9；
The intensity of illumination of the light irradiation apparatus 5 of first culture pond 9 is 8000lx, daily light application time 16h.
After culture according to the method described above, large-scale branch pin class zooplankter can capture once every 2.4 days.It can obtain every time
Take large-scale branch pin class zooplankter 300g.
By above example the result shows that, using the invention carry out culture can be stable acquisition large size branch pin class swim it is dynamic
Object.
The present invention is not limited to the above embodiments, and the above embodiments and description only describe of the invention
Principle, various changes and improvements may be made to the invention without departing from the spirit and scope of the present invention, these change and change
Into all fall within the protetion scope of the claimed invention.
Claims (6)
1. a kind of large size branch pin class zooplankter high-density breeding method, which comprises the following steps:
Prepare base fertilizer:
The base fertilizer is prepared from the following raw materials in parts by weight: 4 ~ 6 parts of chicken manure, 5 ~ 6 parts of pig manure, 0.5 ~ 1 part of leavening, stalk 1 ~
2 parts；Wherein, chicken manure, pig manure and stalk are measured with dry weight；
The preparation step of base fertilizer specifically: weigh each raw material in parts by weight, be uniformly mixed, closed composting 40 ~ 50 days；The composting phase
Between, 60 ~ 70 DEG C are risen to central temperature, turning is primary after heat preservation 3 ~ 5 days；
(2) following three kinds of nutritional agents are configured:
The nutritional agents of chlorella is mixed according to the raw material of following parts by weight: 19 ~ 21 parts of potassium nitrate, potassium dihydrogen phosphate 7 ~ 9
Part, 0.8 ~ 1.2 part of microelement A, VB10.05 ~ 1.1 part, VB120.03 ~ 0.07 part；
The nutritional agents of flat algae is mixed according to the raw material of following parts by weight: 11 ~ 13 parts of sodium nitrate, 5 ~ 7 parts of potassium dihydrogen phosphate,
15 ~ 17 parts of sodium bicarbonate, 1 ~ 3 part of microelement A, VB10.2 ~ 0.4 part, VB120.15 ~ 0.25 part；
The nutritional agents of bread microzyme is mixed according to the raw material of following parts by weight: 9 ~ 11 parts of starch, microelement B 0.1
~ 0.3 part；
(3) cultivation apparatus is built:
The cultivation apparatus includes the first culture pond and covers in rack platform above the first culture pond, the rack platform packet
The pillar that left and right is oppositely arranged is included, is provided with horizontal platen on pillar；Is equipped on the left side strut of the rack platform
The display of one transmissometer, the first transmissometer is mounted on the left side strut of rack platform, and energy measuring temperature, and described first is turbid
The probe of degree instrument is mounted on the left side wall of the first culture pond, is equipped with dissolved oxygen meter on the first culture pond right side wall, the
The middle and lower part of one culture pond is equipped with micropore aeration pipe, light irradiation apparatus is equipped with above the first culture pond, and light irradiation apparatus is pacified
On lower plane loaded on platen, the right side wall lower portion of the first culture pond is equipped with outlet conduit, is equipped with first above pipeline
Valve；The platen upper surface has been sequentially placed the second culture pond, third culture pond and the 4th culture pond from left to right, on platen
Surface is sequentially installed with the second transmissometer, third transmissometer and the 4th transmissometer, the probe of second transmissometer from left to right
It is mounted on the left side wall of the second culture pond, the probe of the third transmissometer is mounted on the left side wall of third culture pond, institute
The probe for stating the 4th transmissometer is mounted on the left side wall of the 4th culture pond, the second culture pond, third culture pond and the 4th culture
The right side wall lower part in pond is mounted on outlet conduit, and valve is equipped on outlet conduit, and the outlet conduit is passed down through platen
And extend in the first culture pond, blender is mounted in the second culture pond, third culture pond and the 4th culture pond；
(4) by bottom in base fertilizer the first culture pond of tiling, add in the second culture pond, third culture pond and the 4th culture pond respectively
The nutritional agents for entering the nutritional agents of chlorella, the nutritional agents of flat algae and bread microzyme, to the first culture pond, the second culture pond,
It is separately added into water of the aeration after 24 hours in three culture ponds and the 4th culture pond, opens the second culture pond, third culture pond and the
Blender in four culture ponds, revolving speed are 6 ~ 8 r/s；
(5) it is inoculated with:
Acquire large-scale branch pin class animal with the zooplankters of 120 ~ 160 mesh acquisition net, be put into the first culture pond, by chlorella,
Flat algae concentrate is separately added into the second culture pond, third culture pond, and inoculum density is 300/L, addition face in the 4th culture pond
Packet yeast dry powder, inoculum concentration 5g/L；
(6) aquaculture management:
After inoculation, according to the growing state of branch pin class zooplankter large-scale in the first culture pond, valve is periodically opened, by the second training
Support pond, third culture pond, the mixed liquor in the 4th culture pond are discharged into the first culture pond；Reach to the first culture pond turbidity
20NTU collects large-scale branch pin class zooplankter, and turbidity stops collecting after being down to 5NTU in the first culture pond.
2. large size branch pin class zooplankter high-density breeding method according to claim 1, it is characterised in that: the first culture
In 4mg/L ~ 6mg/L, temperature is controlled at 25 ~ 28 DEG C Dissolved Oxygen concentration Control in pond.
3. large size branch pin class zooplankter high-density breeding method according to claim 1, it is characterised in that: light irradiation apparatus
Intensity of illumination be 5000lx ~ 8000lx, daily 8 ~ 16h of light application time.
4. large size branch pin class zooplankter high-density breeding method according to claim 1, it is characterised in that: the fermentation
Bacterium uses EM bacterium.
5. large size branch pin class zooplankter high-density breeding method according to claim 1, it is characterised in that: the first culture
The glass production that pond, the second culture pond, third culture pond and the 4th culture pond are all made of with a thickness of 1cm ~ 2.5cm.
6. large size branch pin class zooplankter high-density breeding method according to claim 1, it is characterised in that: described micro
Elements A includes 1 part of ferrous sulfate, 2 parts of calcium chloride, 1.5 parts of zinc sulfate and 0.8 part of manganese dioxide；The microelement B includes chlorine
1.6 parts of calcium of change, 3 parts of inositol, 0.8 part of copper sulphate and 2.5 parts of Tyiamine Hd；The component content of microelement dry weight meter.
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| CN113197133A (en) * | 2021-05-13 | 2021-08-03 | 珠江水利委员会珠江水利科学研究院 | A integration culture apparatus for zooplankton hatching breeds |
| CN117678542A (en) * | 2023-12-21 | 2024-03-12 | 武汉市天泉慧源环保科技有限公司 | A kind of cultivation method of large Daphnia |
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