CN110199616A - It is a kind of using percentage of seedgermination as the ecological safety threshold value experimental machine of toxicity assessment terminal - Google Patents
It is a kind of using percentage of seedgermination as the ecological safety threshold value experimental machine of toxicity assessment terminal Download PDFInfo
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- 230000001988 toxicity Effects 0.000 title claims abstract description 12
- 231100000419 toxicity Toxicity 0.000 title claims abstract description 12
- 230000007226 seed germination Effects 0.000 title claims description 12
- 230000035784 germination Effects 0.000 claims abstract description 49
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 19
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 19
- 239000001301 oxygen Substances 0.000 claims abstract description 19
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims abstract description 18
- 238000011156 evaluation Methods 0.000 claims abstract description 11
- 229910002092 carbon dioxide Inorganic materials 0.000 claims abstract description 9
- 239000001569 carbon dioxide Substances 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims description 13
- 235000015097 nutrients Nutrition 0.000 claims description 13
- 238000002474 experimental method Methods 0.000 claims description 10
- 239000007789 gas Substances 0.000 claims description 4
- 238000004321 preservation Methods 0.000 claims description 3
- 230000008018 melting Effects 0.000 claims 1
- 238000002844 melting Methods 0.000 claims 1
- 239000003595 mist Substances 0.000 claims 1
- 238000005192 partition Methods 0.000 abstract description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 11
- 241000196324 Embryophyta Species 0.000 description 7
- 238000000034 method Methods 0.000 description 7
- 239000002699 waste material Substances 0.000 description 6
- 230000007613 environmental effect Effects 0.000 description 3
- 241000894007 species Species 0.000 description 3
- 231100000048 toxicity data Toxicity 0.000 description 3
- 239000002028 Biomass Substances 0.000 description 2
- 230000035945 sensitivity Effects 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 231100000820 toxicity test Toxicity 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 230000036284 oxygen consumption Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
- A01C1/02—Germinating apparatus; Determining germination capacity of seeds or the like
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- G—PHYSICS
- G05—CONTROLLING; REGULATING
- G05D—SYSTEMS FOR CONTROLLING OR REGULATING NON-ELECTRIC VARIABLES
- G05D27/00—Simultaneous control of variables covered by two or more of main groups G05D1/00 - G05D25/00
- G05D27/02—Simultaneous control of variables covered by two or more of main groups G05D1/00 - G05D25/00 characterised by the use of electric means
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Abstract
本发明属于植物无土栽培技术领域，涉及一种无土栽培实验装置，尤其是一种以种子发芽率为毒性评价终点的生态安全阈值实验机，包括一桁架，所述桁架内间隔安装有多个隔板，多个隔板将桁架均匀分隔为发芽箱，每个发芽箱的中部均安装有一旋转台，旋转台上端部安装有托盘，该托盘可与旋转台同轴同速转动，所述托盘内嵌装有多个培养皿，位于各培养皿之间的托盘中部位置安装有一集成式的传感器，该集成式的传感器可用于实时采集相应发芽箱内的温度、湿度、光照强度和二氧化碳浓度的信息；每个发芽箱内还安装光源、热源和氧气补给装置；所述集成式的传感器的信号输出端，以及光源、热源和氧气补给装置的控制端均与外部的中控箱相连接。
The invention belongs to the technical field of soilless cultivation of plants, and relates to a soilless cultivation experimental device, in particular to an ecological safety threshold experimental machine whose toxicity evaluation endpoint is based on the germination rate of seeds. Multiple partitions evenly divide the truss into germination boxes. A rotating table is installed in the middle of each germination box. A tray is installed on the upper end of the rotating table. The tray can rotate coaxially and at the same speed as the rotating table. There are multiple petri dishes embedded in the tray, and an integrated sensor is installed in the middle of the tray between each petri dish, which can be used to collect the temperature, humidity, light intensity and carbon dioxide concentration in the corresponding germination box in real time information; light source, heat source and oxygen supply device are also installed in each germination box; the signal output end of the integrated sensor, and the control end of light source, heat source and oxygen supply device are all connected with the external central control box.
Description
技术领域technical field
本发明属于植物无土栽培技术领域，涉及一种无土栽培实验装置，尤其是一种以种子发芽率为毒性评价终点的生态安全阈值实验机。The invention belongs to the technical field of plant soilless cultivation, and relates to a soilless cultivation experiment device, in particular to an ecological safety threshold experiment machine whose toxicity evaluation endpoint is based on the germination rate of seeds.
背景技术Background technique
植物种子发芽首先吸水膨胀，随着种子吸水膨胀直至露白，呼吸作用逐渐加强，需要大量的氧气，但是植物种子长期浸泡在水中会使种子无法进行呼吸，导致种子不能发芽，所以需要反复地向发芽箱中加水和放水，这会增加劳动力成本，且放水和加水的时间不当还会导致无法发芽成功。同时，浸泡种子使用的水长时间静置，水中的细菌滋生快，水的稠度会变大，对种子发芽也会产生不利影响。此外，每一种作物的种子都有发芽所需的最低温度、最适温度以及最高温度，在最低温度以下，种子萌发慢或者不萌发，在最高温度以上，可能会出现烧苗或者幼苗徒长不健壮，只有在最适温度下萌发，种子发芽才最佳。The germination of plant seeds first absorbs water and swells. As the seeds absorb water and swell until they are white, the respiration gradually strengthens and requires a lot of oxygen. However, plant seeds soaked in water for a long time will make the seeds unable to breathe and cause the seeds to fail to germinate. Therefore, it is necessary to repeatedly germinate Add water and release water in the box, which will increase labor costs, and improper time of water release and water addition will also lead to failure to germinate successfully. At the same time, if the water used to soak the seeds is left standing for a long time, the bacteria in the water will grow rapidly, and the consistency of the water will increase, which will also have an adverse effect on the germination of the seeds. In addition, the seeds of each crop have a minimum temperature, an optimum temperature, and a maximum temperature required for germination. Below the minimum temperature, the seeds germinate slowly or not, and above the maximum temperature, there may be burning or leggy seedlings Robust, the seeds germinate best only when germinated at the optimum temperature.
物种敏感性分布法(Species Sensitivity Distribution，SSD)需要以多物种的毒性数据作支撑，但是目前获取毒性数据方法大多是以植物生物量或植物根伸长作为毒性评价终点进行毒性实验，植物生物量及植物根伸长实验均需要耗费大量的人力物力，以多物种进行毒性实验时占用空间较大，而且对植物生长过程中的外界环境因素难以精确控制，比如温度、湿度、光照强度以及耗氧量等，进而使得到的毒性数据质量较差。The species sensitivity distribution method (Species Sensitivity Distribution, SSD) needs to be supported by multi-species toxicity data, but most of the current toxicity data acquisition methods use plant biomass or plant root elongation as the toxicity evaluation endpoint to conduct toxicity experiments. Plant biomass and plant root elongation experiments require a lot of manpower and material resources, and it takes up a lot of space when conducting toxicity experiments with multiple species, and it is difficult to accurately control external environmental factors during plant growth, such as temperature, humidity, light intensity, and oxygen consumption. amount, etc., resulting in poor quality of the toxicity data obtained.
种子发芽过程中除了控制好温度及湿度外，还需要对种子发芽情况进行观察，并对种子的发芽率进行统计，对于现有的种子发芽设备对种子发芽情况及种子发芽率进行统计时，均是通过人工的方式对每个发芽箱进行逐个观察和统计，这样的工作方式不仅工作量多、劳动强度大而且容易出错。In addition to controlling the temperature and humidity during the seed germination process, it is also necessary to observe the germination of the seeds and make statistics on the germination rate of the seeds. It is to observe and count each germination box one by one by manual method. This kind of work method not only has a lot of work, but also is labor-intensive and error-prone.
发明内容Contents of the invention
本发明的目的在于克服现有技术的不足，提供一种减少人工参与，可实时采集发芽箱内的温度、湿度、二氧化碳浓度等信息，并进行补偿和调节的以种子发芽率为毒性评价终点的生态安全阈值实验机。The purpose of the present invention is to overcome the deficiencies of the prior art, to provide a kind of reducing artificial participation, can collect the information such as temperature, humidity, carbon dioxide concentration in the germination box in real time, and carry out compensation and adjustment with the toxicity evaluation end point of seed germination rate Ecological safety threshold test machine.
本发明采取的技术方案是：The technical scheme that the present invention takes is:
一种以种子发芽率为毒性评价终点的生态安全阈值实验机，包括一桁架，所述桁架内间隔安装有多个隔板，多个隔板将桁架均匀分隔为发芽箱，所述桁架外部设置有一营养液箱，该营养液箱的出液管与每个发芽箱的侧壁均安装有一接口，接口内安装有雾化管，通过雾化管可向每个发芽箱内喷淋雾化后的营养液，其特征在于：每个发芽箱的中部均安装有一旋转台，旋转台上端部安装有托盘，该托盘可与旋转台同轴同速转动，所述托盘内嵌装有多个培养皿，位于各培养皿之间的托盘中部位置安装有一集成式的传感器，该集成式的传感器可用于实时采集相应发芽箱内的温度、湿度、光照强度和二氧化碳浓度的信息；每个发芽箱内还安装光源、热源和氧气补给装置；所述集成式的传感器的信号输出端，以及光源、热源和氧气补给装置的控制端均与外部的中控箱相连接。An ecological safety threshold experimental machine with a seed germination rate toxicity evaluation endpoint, including a truss, a plurality of partitions are installed at intervals in the truss, and a plurality of partitions evenly divide the truss into germination boxes, and the outside of the truss is set There is a nutrient solution tank, the liquid outlet pipe of the nutrient solution tank is equipped with an interface with the side wall of each germination box, and an atomizing tube is installed in the interface, through which the atomized spray can be sprayed into each germination box The nutrient solution is characterized in that: a rotating table is installed in the middle of each germination box, and a tray is installed on the upper end of the rotating table, and the tray can rotate coaxially and at the same speed as the rotating table. An integrated sensor is installed in the middle of the tray between the petri dishes. This integrated sensor can be used to collect the information of temperature, humidity, light intensity and carbon dioxide concentration in the corresponding germination box in real time; A light source, a heat source and an oxygen supply device are also installed; the signal output end of the integrated sensor, and the control ends of the light source, heat source and oxygen supply device are all connected to the external central control box.
而且，所述光源为采用亮度可调的白炽灯，白炽灯安装在每个发芽箱的顶部；所述热源采用一保温灯。Moreover, the light source is an incandescent lamp with adjustable brightness, and the incandescent lamp is installed on the top of each germination box; the heat source is a heat preservation lamp.
而且，所述氧气补给系统包括一导气管，该导气管与一输氧泵的出气端相连接，所述导气管制有多个出气口，每个出气口均布置在相应发芽箱内。Moreover, the oxygen supply system includes an air guide tube, which is connected to an air outlet of an oxygen pump, and the air guide tube has a plurality of air outlets, and each air outlet is arranged in a corresponding germination box.
而且，每个所述的发芽箱内均安装有一摄像头，每个摄像头的数据输出端均与外部的中控箱相连接。Moreover, a camera is installed in each of the germination boxes, and the data output end of each camera is connected with the external central control box.
而且，每个所述的发芽箱侧壁内均安装有一排气扇，驱动排气扇的电机控制端和外部的中控箱相连接。Moreover, an exhaust fan is installed in each of the germination box side walls, and the motor control end for driving the exhaust fan is connected with the external central control box.
本发明的优点和积极效果是：Advantage and positive effect of the present invention are:
本发明中，旋转盘配合托盘带动其内部嵌装的培养皿周期性转动，使得每个培养皿内的种子受光均匀，有效避免因种子受光不均匀而产生的实验误差，集成式的传感器用于实时采集发芽箱内的环境信息，包括温度，湿度，光照强度和二氧化碳浓度，并将采集的信息实时反馈至中控箱内，中控箱根据采集的数据信息，对光源、热源和氧气补给装置输出控制命令，使得发芽箱内始终处于适合种子发芽的最优状态。In the present invention, the rotating disk cooperates with the tray to drive the petri dish embedded in it to rotate periodically, so that the seeds in each petri dish receive light evenly, effectively avoiding the experimental error caused by the uneven light receiving of the seeds, and the integrated sensor is used for The environmental information in the germination box is collected in real time, including temperature, humidity, light intensity and carbon dioxide concentration, and the collected information is fed back to the central control box in real time. The central control box controls the light source, heat source and oxygen supply device according to the collected data information. Output control commands, so that the germination box is always in the optimal state suitable for seed germination.
附图说明Description of drawings
图1为本发明的结构示意图。Fig. 1 is a structural schematic diagram of the present invention.
具体实施方式Detailed ways
下面结合实施例，对本发明进一步说明，下述实施例是说明性的，不是限定性的，不能以下述实施例来限定本发明的保护范围。Below in conjunction with the examples, the present invention is further described, the following examples are illustrative, not limiting, and the protection scope of the present invention cannot be limited by the following examples.
一种以种子发芽率为毒性评价终点的生态安全阈值实验机，包括一桁架5，所述桁架内间隔安装有多个隔板，多个隔板将桁架均匀分隔为发芽箱，所述桁架外部设置有一营养液箱10，该营养液箱的出液管与每个发芽箱的侧壁均安装有一接口，接口内安装有雾化管23，每个雾化内均间隔安装有多个雾化喷头。通过雾化喷头可向每个发芽箱内喷淋雾化后的营养液，本发明的创新在于，每个发芽箱的中部均安装有一旋转台15，旋转台旁侧安装有用于驱动的减速电机14，旋转台上端部安装有托盘16，该托盘可与旋转台同轴同速转动，所述托盘内嵌装有多个培养皿，位于各培养皿之间的托盘中部位置安装有一集成式的传感21，该集成式的传感器可用于实时采集相应发芽箱内的温度、湿度、光照强度和二氧化碳浓度的信息；每个发芽箱内还安装光源、热源和氧气补给装置；所述集成式的传感器的信号输出端，以及光源、热源和氧气补给装置的控制端均与外部的中控箱19相连接。A kind of ecological safety threshold experiment machine with seed germination rate toxicity evaluation endpoint, comprising a truss 5, a plurality of partitions are installed at intervals in the truss, and a plurality of partitions evenly divide the truss into germination boxes, and the outside of the truss A nutrient solution tank 10 is provided, and an interface is installed between the outlet pipe of the nutrient solution tank and the side wall of each germination box, and an atomizing tube 23 is installed in the interface, and multiple atomizing tubes 23 are installed in each atomizing box at intervals. nozzle. The atomized nutrient solution can be sprayed into each germination box through the atomizing nozzle. The innovation of the present invention is that a rotating table 15 is installed in the middle of each germination box, and a geared motor for driving is installed on the side of the rotating table. 14. A tray 16 is installed on the upper end of the turntable, and the tray can rotate coaxially and at the same speed as the turntable. The tray is embedded with a plurality of petri dishes, and an integrated tray is installed in the middle of each petri dish. Sensing 21, the integrated sensor can be used to collect the information of temperature, humidity, light intensity and carbon dioxide concentration in the corresponding germination box in real time; light source, heat source and oxygen supply device are also installed in each germination box; the integrated The signal output terminal of the sensor, and the control terminals of the light source, heat source and oxygen supply device are all connected with the external central control box 19 .
本实施例中，所述光源为采用亮度可调的白炽灯4，白炽灯安装在每个发芽箱的顶部；所述热源采用一保温灯7。In this embodiment, the light source is an incandescent lamp 4 with adjustable brightness, and the incandescent lamp is installed on the top of each germination box; a heat preservation lamp 7 is used as the heat source.
本实施例中，所述氧气补给系统包括一导气管1，该导气管与一输氧泵3的出气端相连接，所述导气管制有多个出气口22，每个出气口均布置在相应发芽箱内。In this embodiment, the oxygen supply system includes an air guide tube 1, which is connected to the gas outlet end of an oxygen pump 3, and the air guide tube has a plurality of gas outlets 22, and each gas outlet is arranged at a corresponding Inside the germination box.
本实施例中，每个所述的发芽箱内均安装有一摄像头20，每个摄像头的数据输出端均与外部的中控箱相连接，每个摄像头的数据输出端和中控箱相连接。由于摄像头位于湿度很大的试验仓内，因此，该摄像头优选采用IP66及以上具有防水性能的摄像头，该摄像头也可采用常规摄像头，但需在摄像头迎水面一侧安装隔水挡板或遮水帘等装置。In the present embodiment, a camera 20 is installed in each germination box, and the data output end of each camera is connected with the external central control box, and the data output end of each camera is connected with the central control box. Since the camera is located in the test chamber with high humidity, it is preferable to use a camera with waterproof performance of IP66 or above. The camera can also use a conventional camera, but it is necessary to install a water-proof baffle or water-shielding on the side of the camera facing the water. curtains etc.
本实施例中，每个所述的发芽箱侧壁内均安装有一排气扇6，驱动排气扇的电机控制端和外部的中控箱相连接。In this embodiment, an exhaust fan 6 is installed in the side wall of each germination box, and the motor control end for driving the exhaust fan is connected with the external central control box.
本实施例中，在桁架顶部安装有一遮雨棚2；在桁架底部安装有一废液箱17，每个所述的发芽箱底部均制出一废液排出口，发芽箱底部制有坡面13，由制有废液排出口一侧向另一侧逐步提升，所述废液排出口通过废液管18与废液箱相连通。In this embodiment, a rain shelter 2 is installed at the top of the truss; a waste liquid tank 17 is installed at the bottom of the truss, and a waste liquid discharge port is made at the bottom of each germination box, and a slope 13 is formed at the bottom of the germination box , from the side where the waste liquid outlet is formed to gradually lift to the other side, and the waste liquid outlet communicates with the waste liquid tank through the waste liquid pipe 18 .
本实施例中，所述营养液箱的出液管9内安装有泵机11和流量控制器12，所述出液管的另一侧分为多个支管，每个支管内均安装有单向电磁阀8，各支管的端部均制有出液口，每个出液口均连通安装有一雾化管，雾化管内间隔间隔安装有多个雾化喷头。In this embodiment, a pump 11 and a flow controller 12 are installed in the liquid outlet pipe 9 of the nutrient solution tank, and the other side of the liquid outlet pipe is divided into a plurality of branch pipes, each of which is equipped with a single To the electromagnetic valve 8, the end of each branch pipe is all shaped with a liquid outlet, and each liquid outlet is connected with an atomizing tube installed, and a plurality of atomizing nozzles are installed at intervals in the atomizing tube.
本实施例中，在营养液箱内安装有液位传感器。In this embodiment, a liquid level sensor is installed in the nutrient solution tank.
本实施例中，集成式传感器包括温湿度传感器，优选采用钱涌ZZ-S-TH-A，PH传感器优选采用陆恒RS485输出，光照强度传感器优选采用BH1750FVI和二氧化碳传感器优选采用MH-Z14A，，所述传感器的信号输出端和中控箱相连通。In this embodiment, the integrated sensor includes a temperature and humidity sensor, preferably Qianyong ZZ-S-TH-A, the pH sensor preferably uses Luheng RS485 output, the light intensity sensor preferably uses BH1750FVI and the carbon dioxide sensor preferably uses MH-Z14A, The signal output end of the sensor communicates with the central control box.
本实施例中，所述中控箱包括PCB、PLC、继电器、开关、控制面板以及若干接线端子形成一控制装置，PCB的输入端转配有A/D转换器，所述电磁式传感器，集成式的传感器均输出模拟信号，输出的模拟信号经A/D转换器转换后传输至PLC。In this embodiment, the central control box includes a PCB, PLC, relays, switches, a control panel, and several terminals to form a control device, the input end of the PCB is equipped with an A/D converter, and the electromagnetic sensor is integrated All sensors of the type output analog signals, and the output analog signals are converted by the A/D converter and then transmitted to the PLC.
本发明的工作过程是：Working process of the present invention is:
本发明使用时，将内置有种子的培养皿放置于托盘内，之后开启减速电机，带动托盘整体旋转，其转速优选有1r/min，此时，集成式的传感器，实时检测发芽箱内的温度，湿度，二氧化碳浓度和光照强度等信息；当某一数值低于设定值时，该模拟信号输出至PLC，PLC采集该信号后向光源、热源、氧气补给系统或营养液补给系统输出相应控制命令，白炽灯、热源灯、输氧泵和营养液的泵机则分别启动进行补充。摄像头则用于实时采集实验箱内的相应信息，并将精确的测量结果进行显示，储存等，进而得到准确及时的实验数据。When using the present invention, place the culture dish with seeds inside the tray, and then turn on the deceleration motor to drive the tray to rotate as a whole. The rotation speed is preferably 1r/min. At this time, the integrated sensor detects the temperature in the germination box in real time , humidity, carbon dioxide concentration and light intensity and other information; when a certain value is lower than the set value, the analog signal is output to the PLC, and the PLC outputs the corresponding control to the light source, heat source, oxygen supply system or nutrient solution supply system after collecting the signal command, the pumps for incandescent lamps, heat source lamps, oxygen pumps, and nutrient solutions are started separately for replenishment. The camera is used to collect the corresponding information in the experiment box in real time, and display and store the accurate measurement results, so as to obtain accurate and timely experimental data.
本发明中，旋转盘配合托盘带动其内部嵌装的培养皿周期性转动，使得每个培养皿内的种子受光均匀，有效避免因种子受光不均匀而产生的实验误差，集成式的传感器用于实时采集发芽箱内的环境信息，包括温度，湿度，光照强度和二氧化碳浓度，并将采集的信息实时反馈至中控箱内，中控箱根据采集的数据信息，对光源、热源和氧气补给装置输出控制命令，使得发芽箱内始终处于适合种子发芽的最优状态。In the present invention, the rotating disk cooperates with the tray to drive the petri dish embedded in it to rotate periodically, so that the seeds in each petri dish receive light evenly, effectively avoiding the experimental error caused by the uneven light receiving of the seeds, and the integrated sensor is used for The environmental information in the germination box is collected in real time, including temperature, humidity, light intensity and carbon dioxide concentration, and the collected information is fed back to the central control box in real time. The central control box controls the light source, heat source and oxygen supply device according to the collected data information. Output control commands, so that the germination box is always in the optimal state suitable for seed germination.
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Application publication date: 20190906 |