CN119959435B - A chromatographic column tube structure provided with a flow guiding structure - Google Patents
A chromatographic column tube structure provided with a flow guiding structure Download PDFInfo
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Abstract
本申请公开了一种设有导流结构的谱柱柱管结构，具体涉及色谱柱技术领域，包括柱管主体、第一导流组件和第二导流组件，所述柱管主体的进水端连接有第一导流组件，且所述柱管主体的出水端连接有第二导流组件，且所述柱管主体包含有管壁、螺旋导流槽和微型扰流片，且所述管壁的内壁设置有螺旋导流槽，且所述管壁远离第一导流组件的一侧设置有微型扰流片，因此当样品沿柱管主体内的螺旋导流槽进行流动时，螺旋导流槽会强制样品沿螺旋路径流动，增强径向混合，减少偏流和死体积，同时所设置的微型扰流片对柱管主体内的样品在流动后产生局部湍流，破坏层流边界层，提高传质效率。
The present application discloses a chromatographic column tube structure provided with a flow guide structure, specifically relating to the technical field of chromatographic columns, comprising a column tube body, a first flow guide component and a second flow guide component, wherein the water inlet end of the column tube body is connected to the first flow guide component, and the water outlet end of the column tube body is connected to the second flow guide component, and the column tube body comprises a tube wall, a spiral flow guide groove and a micro-spoiler, and the inner wall of the tube wall is provided with a spiral flow guide groove, and the side of the tube wall away from the first flow guide component is provided with a micro-spoiler, so when the sample flows along the spiral flow guide groove in the column tube body, the spiral flow guide groove forces the sample to flow along a spiral path, thereby enhancing radial mixing, reducing bias flow and dead volume, and at the same time, the provided micro-spoiler generates local turbulence for the sample in the column tube body after the flow, destroys the laminar boundary layer, and improves the mass transfer efficiency.
Description
Technical Field
The application relates to the technical field of chromatographic columns, in particular to a chromatographic column tube structure with a flow guide structure.
Background
The chromatographic column is a key component for chromatographic analysis, and the separation of components in a mixture is realized through the difference of partition coefficients of different substances between a stationary phase and a mobile phase, so that the performance and the application range of the chromatographic column are further improved along with the continuous progress of material science and analysis technology in recent years, and the separation capability of the chromatographic column on complex samples is stronger. Meanwhile, miniaturization and high-flux technology of the chromatographic column are also rapidly developed, so that chromatographic analysis is more efficient and rapid, but problems such as bias flow or dead volume of a sample often occur in the chromatographic column;
In the conventional publication No. CN109891232A, a column for chromatography is disclosed, and a column in which a packing material is packed in an optimally compressed state to prevent leakage of a mobile phase containing a test body from a tube is disclosed. The pipe column mainly comprises a cylindrical pipe, a pipe plug, a first filter, a second filter, a plug and a connector. The pipe mainly comprises a cylindrical containing part with a bottom and a cylindrical bottom side connecting part connected with the bottom of the containing part. The receiving portion and the bottom-side connecting portion are disposed coaxially. The plug mainly includes a cylindrical outer tube portion coaxially provided, and an inner tube portion provided inside the outer tube portion. The first filter and the second filter are filters of such an extent that the filler material cannot pass through. The plug mainly comprises a plug holding part and a plug inserting part. The connector mainly comprises a connector holding part and a connector inserting part. The inventors found that the following problems exist in the prior art in the process of implementing the present application:
When the existing spectrum column is used again, bias flow and dead volume often occur, wherein the bias flow can lead to uneven distribution of samples in the column, the separation effect is poor, the peak shape is widened or trailing is caused, the dead volume can lead to retention of samples in certain areas in the column, the samples cannot be effectively participated in separation, and the analysis time is prolonged or the peak shape is not ideal;
therefore, a chromatographic column tube structure with a diversion structure is provided for the problems.
Disclosure of Invention
In order to overcome the above-mentioned drawbacks of the prior art, the present application provides a chromatographic column tube structure with a flow guiding structure, so as to solve the above-mentioned problems in the prior art.
The chromatographic column tube structure comprises a column tube main body, a first flow guide component and a second flow guide component, wherein the water inlet end of the column tube main body is connected with the first flow guide component, the water outlet end of the column tube main body is connected with the second flow guide component, the column tube main body comprises a tube wall, a spiral flow guide groove and a miniature spoiler, the inner wall of the tube wall is provided with the spiral flow guide groove which forces a sample to advance along a spiral path, one side of the tube wall far away from the first flow guide component is provided with the miniature spoiler which generates local turbulence to the flow of the sample and damages a laminar boundary, and the outer surface of the miniature spoiler is provided with the miniature bulge.
Preferably, the column tube main body comprises a temperature control element, a micro pressure sensor, an optical window and a conveying cavity, the temperature control element is arranged on the outer diameter surface of the column tube main body, the micro pressure sensor is arranged below the temperature control element, the optical window is arranged below the micro pressure sensor, and the conveying cavity is arranged on one side, far away from the temperature control element, of the spiral diversion trench.
Preferably, the column tube main body further comprises a first preassembling interface, a second preassembling interface and an external thread wall, the first preassembling interface is installed at the top end of the column tube main body, the second preassembling interface is arranged on one side, far away from the first preassembling interface, of the column tube main body, and the external thread wall is arranged at one end, far away from the first preassembling interface, of the second preassembling interface.
Preferably, the first diversion component comprises a fixed collar, an adsorption ring, a pressurizing pipe, a first mounting ring and a connecting pipe, wherein the adsorption ring is arranged at one end of the fixed collar far away from the column pipe main body, the pressurizing pipe is arranged at one end of the adsorption ring far away from the fixed collar, the first mounting ring is arranged at one end of the pressurizing pipe far away from the adsorption pipe, and the connecting pipe is arranged at one end of the first mounting ring far away from the pressurizing pipe.
Preferably, the second diversion component comprises a locking ring, an internal thread wall and a locking shaft, the internal thread wall is arranged at the edge of the inner wall of the locking ring, and the side edge of the locking ring is connected with the locking shaft.
Preferably, the second diversion component further comprises an outer connecting pipe, a mounting pipe and an inserting pipe, wherein the mounting pipe is mounted at one end, far away from the locking ring, of the outer connecting pipe, and the inserting pipe is mounted at one end, far away from the outer connecting pipe, of the mounting pipe.
Preferably, the inner wall both ends of pillar main part are all pegged graft and are had grafting injection core, just peg graft injection core and include sealing ring, inlet, solid fixed ring, feed liquor pipe, second collar and fixed pipe, just the pillar main part with place the sealing ring between the grafting injection core, just the center department of sealing ring runs through and is provided with the inlet, just the feed liquor pipe has been placed to one side that the pillar main part was kept away from to the inlet, just the external diameter surface cover of feed liquor pipe is equipped with solid fixed ring, just gu fixed ring's external diameter surface cover is equipped with the second collar, just one side that the sealing ring was kept away from to the second collar is provided with solid fixed pipe.
Preferably, the one end that the inlet was kept away from to the pillar tube main part is provided with the liquid outlet, just the drain pipe has been placed to the one end that the inlet was kept away from to the liquid outlet, just the external diameter surface cover of drain pipe is equipped with the third collar.
Preferably, a docking ring is installed between the column pipe main body and the first flow guiding component, a detachable structure is formed between the column pipe main body and the first flow guiding component through the docking ring, and a detachable structure is formed between the column pipe main body and the second flow guiding component through an external thread wall and an internal thread wall.
The application has the technical effects and advantages that:
1. Compared with the prior art, the chromatographic column tube structure with the flow guide structure comprises a column tube main body, a first flow guide assembly and a second flow guide assembly, wherein when the column tube main body structure is used, when a sample enters the inside of the column tube main body, a spiral flow guide groove is formed in the wall of the inner wall of the column tube main body, the sample is forced to advance along a spiral path, a more uniform and stable flow mode is formed in the column by guiding the sample and a mobile phase, so that the separation performance is improved, and a micro spoiler is arranged in the wall of the column to adjust the flow property of the sample in the column tube main body.
2. Compared with the prior art, the chromatographic column tube structure with the flow guiding structure has the advantages that when a sample flows along the spiral flow guiding groove in the column tube main body, the spiral flow guiding groove can force a mobile phase to flow along the spiral path, radial mixing is enhanced, bias flow and dead volume are reduced, and meanwhile, the micro spoiler generates local turbulence to the sample in the column tube main body after flowing, a laminar boundary layer is damaged, and transmission efficiency is improved.
Drawings
FIG. 1 is a schematic diagram of the overall structure of the present application;
FIG. 2 is a schematic elevational view in cross-section of the vial body of the present application;
FIG. 3 is a schematic cross-sectional front view of a micro spoiler according to the present application;
FIG. 4 is a schematic cross-sectional elevation view of a plug-in inject core according to the application;
FIG. 5 is a schematic diagram of the structure of FIG. 3A according to the present application;
FIG. 6 is a schematic diagram of the structure of the present application at B in FIG. 4;
FIG. 7 is a schematic view of a docking collar of the present application;
FIG. 8 is a schematic view of the structure of the pipe wall of the present application;
fig. 9 is a schematic structural view of the locking ring of the present application.
The device comprises a column pipe main body, 101, a pipe wall, 102, a spiral diversion groove, 103, a temperature control element, 104, a micro pressure sensor, 105, an optical window, 106, a conveying cavity, 107, a micro spoiler, 108, a first preassembled interface, 109, a second preassembled interface, 1010, an external thread wall, 2, a first diversion component, 201, a fixed collar, 202, an adsorption ring, 203, a pressurizing pipe, 204, a first installation ring, 205, a connecting pipe, 3, a second diversion component, 301, a locking ring, 302, an internal thread wall, 303, a locking shaft, 304, an external pipe, 305, an installation pipe, 306, an insertion pipe, 4, an insertion injection core, 5, a sealing ring, 6, a liquid inlet, 7, a fixed ring, 8, a liquid inlet pipe, 9, a second installation ring, 10, a fixed pipe, 11, a liquid outlet, 12, a liquid outlet pipe, 13, a third installation ring, 14 and a butt joint ring.
Detailed Description
The following description of the embodiments of the present application will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present application, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the application without making any inventive effort, are intended to be within the scope of the application.
Examples
The chromatographic column tube structure with a flow guiding structure as shown in fig. 1 to 9 comprises a column tube main body 1, a first flow guiding component 2 and a second flow guiding component 3, wherein the water inlet end of the column tube main body 1 is connected with the first flow guiding component 2, the water outlet end of the column tube main body 1 is connected with the second flow guiding component 3, the column tube main body 1 comprises a tube wall 101, a spiral flow guiding groove 102 and a micro spoiler 107, the inner wall of the tube wall 101 is provided with the spiral flow guiding groove 102 for forcing a sample to advance along a spiral path, one side of the tube wall 101 far away from the first flow guiding component 2 is provided with the micro spoiler 107 for generating local turbulence to the flow of the sample and damaging a laminar boundary, and the outer surface of the micro spoiler 107 is provided with micro bulges.
Wherein the spectral column tube structure is composed of a column tube main body 1, a first flow guide component 2 and a second flow guide component 3, wherein when the column tube main body 1 is used, when a sample enters the interior of the column tube main body 1, a spiral flow guide groove 102 is arranged on a tube wall 101 of the inner wall of the column tube main body 1, a mobile phase is forced to advance along a spiral path, the sample and the mobile phase are guided to form a more uniform and stable flow mode in the column, so that the separation performance is improved, a micro spoiler 107 is also arranged in the tube wall 101 to adjust the flow property of the sample in the column tube main body 1, so that tiny protrusions arranged in the column generate local turbulence on the flow of the sample, break a laminar boundary layer, avoid mass transfer resistance caused by 'wall-attaching flow' of the mobile phase, change the layer area of the flow of the sample in the column tube main body 1 from laminar flow to be more approximate to plug flow, reducing axial diffusion, reducing theoretical plate height, presenting narrower, more symmetrical chromatographic peaks, improving resolution, and reducing performance fluctuations due to column packing differences or long term use, prolonging column life, while imagining the column as a river, when there is no flow guiding structure inside the column body 1, water flows may form eddies or branches (drift) due to uneven river beds, partial regional water flow stagnations (dead volumes), while when there is a flow guiding structure inside the column body 1, such as a baffle or artificial channel provided in the river beds, guiding water flows uniformly forward, reducing turbulence and stagnation regions, so that when samples flow along the spiral flow guiding grooves 102 inside the column body 1, the spiral flow guiding grooves 102 force mobile phases to flow along spiral paths, enhance radial mixing, reduce drift and dead volumes, meanwhile, the arranged micro spoiler 107 generates local turbulence on the sample in the pillar tube main body 1 after flowing, and damages a laminar boundary layer, thereby improving mass transfer efficiency.
Examples
The scheme in example 1 is further elaborated on the basis of example 1 in connection with the following specific working modes, as shown in fig. 1 to 9, described in detail below:
As a preferred embodiment, the column tube main body 1 comprises a temperature control element 103, a micro pressure sensor 104, an optical window 105 and a conveying cavity 106, the temperature control element 103 is arranged on the outer diameter surface of the column tube main body 1, the micro pressure sensor 104 is arranged below the temperature control element 103, the optical window 105 is arranged below the micro pressure sensor 104, the conveying cavity 106 is arranged on one side, far away from the temperature control element 103, of the spiral diversion trench 102, as a preferred embodiment, the column tube main body 1 comprises the temperature control element 103, the micro pressure sensor 104, the optical window 105 and the conveying cavity 106, the temperature control element 103 is arranged on the outer diameter surface of the column tube main body 1, the micro pressure sensor 104 is arranged below the temperature control element 103, the optical window 105 is arranged below the micro pressure sensor 104, and the side of the spiral diversion trench 102 far away from the temperature control element 103 is provided with a conveying cavity 106, and the model of the temperature control element 103 is Custom-thermo electric-12711-5L31-03CL, wherein the temperature control element 103 precisely controls column temperature by integrating resistance wires on the outer wall of the column body 1, optimizes separation selectivity, and the model of the micro pressure sensor 104 is XJC-Z15-H9-2M, wherein the micro pressure sensor 104 monitors column pressure in the first dimension, triggers fraction collection, and feeds back pressure change in real time, adjusts blowback flow rate, then wherein an optical window 105 is provided in the column body 1, allows on-line detection for ultraviolet absorption and fluorescence detection for a part in a sample, wherein the optical window 105 is quartz or sapphire transparent material, and the conveying cavity 106 is a space for conveying the sample by the column body 1, so that the sample passes through the transport chamber 106 and is guided.
As a preferred embodiment, the vial body 1 further comprises a first preassembly port 108, a second preassembly port 109 and an external thread wall 1010, the top end of the vial body 1 is provided with the first preassembly port 108, one side of the vial body 1 far away from the first preassembly port 108 is provided with the second preassembly port 109, one end of the second preassembly port 109 far away from the first preassembly port 108 is provided with the external thread wall 1010, the ports at two ends of the vial body 1 are provided with the first preassembly port 108 and the second preassembly port 109, the first preassembly port 108 and the second preassembly port 109 are identical in structure, and the first preassembly port 108 arranged at the water inlet end of the vial body 1 is used for connecting with the docking collar 14, so that the vial body 1 is quickly connected with the docking collar 14 through the first preassembly port 108, and the risk of dead volume and leakage is reduced.
As a preferred embodiment, the first diversion assembly 2 comprises a fixed collar 201, an adsorption ring 202, a pressurizing pipe 203, a first mounting ring 204 and a connecting pipe 205, wherein the adsorption ring 202 is installed at one end of the fixed collar 201 far away from the column main body 1, the pressurizing pipe 203 is installed at one end of the adsorption ring 202 far away from the fixed collar 201, the first mounting ring 204 is installed at one end of the pressurizing pipe 203 far away from the adsorbing pipe, and the connecting pipe 205 is installed at one end of the first mounting ring 204 far away from the pressurizing pipe 203, wherein the fixed collar 201 is the starting end of the first diversion assembly 2, and is directly connected with the first preassembled interface 108 of the column main body 1, after the first diversion assembly 2 is connected with the column main body 1, the mechanical fixation is provided for the column main body 1, and the coaxiality of the assembly and the column main body 1 is ensured, and the first diversion assembly 2 and the column main body 1 are quickly assembled and disassembled through threads, the sample passes through the inside of the adsorption ring 202 between the first diversion assembly 2 and the column main body 1, wherein when the sample passes through the adsorption ring 202, the sample contacts with the activated carbon in the adsorption ring 202 to trap trace impurities in the sample, the adsorption ring 202 is of a detachable design structure to facilitate regular replacement of the adsorption ring 202, the sample enters the inside of the pressurizing tube 203 before entering the adsorption ring 202, the inside diameter of the pressurizing tube 203 is of a gradual change structure to adjust the pressure of the sample in the process of passing through the adsorption ring 202, the pressurizing tube 203 is linked with an external gas circuit system to control the flow rate through pressure feedback arranged by a box body connected with the column main body 1, the first mounting ring 204 is used for mechanically connecting the connecting tube 205 with the pressurizing tube 203, the first mounting ring 204 provides a standardized interface for adapting to an external device for connection, the connecting pipe 205 is used as a port connection interface of the first flow guiding component 2, the inner wall of the connecting pipe 205 is polished to reduce the dead volume of a flow path of a sample in the first flow guiding component 2, the sample flows out of the connecting pipe 205, the pressurizing pipe 203 connected through the first mounting ring 204, the sample subjected to pressure adjustment, the impurity adsorbed and enters the fixing collar 201, and finally the impurity is conveyed to the downstream column main body 1 by the fixing collar 201, wherein the first flow guiding component 2 adopts metal sealing and the leakage rate is less than or equal to 1 multiplied by 10 < - 9 > Pa.m3/s.
As a preferred embodiment, the second diversion assembly 3 comprises a locking ring 301, an internal thread wall 302 and a locking shaft 303, wherein the internal thread wall 302 is arranged at the edge of the inner wall of the locking ring 301, the locking shaft 303 is connected to the side edge of the locking ring 301, the locking ring 301 is positioned at the edge of the inner wall of the locking ring 301, the specification of the thread is m6x0.75, the locking ring 301 is used for being quickly screwed with the external thread of a column pipe, and the locking ring 301 is directly embedded into the side wall of the locking ring 301, and by adopting an eccentric cam structure, axial pretightening force which is more than or equal to 50N M can be generated during screwing, so that the locking ring is prevented from loosening under high-pressure working conditions.
As a preferred embodiment, the second flow guiding component 3 further comprises an outer connecting tube 304, a mounting tube 305 and a plugging tube 306, wherein the mounting tube 305 is mounted at one end of the outer connecting tube 304 far away from the locking ring 301, the plugging tube 306 is mounted at one end of the mounting tube 305 far away from the outer connecting tube 304, then when a sample enters the second flow guiding component 3 through the column tube main body 1, the sample enters the cavity of the outer connecting tube 304, wherein the outer connecting tube 304 is a transition flow path of the second flow guiding component 3, the inner diameter of the outer connecting tube 304 is matched with the column tube, the tolerance + -0.01 mm is used for avoiding the abrupt change of flow velocity, and the connection between the mounting tube 305 and the plugging tube 306 is used for connecting with equipment.
As a preferred embodiment, the inner wall both ends of the pillar tube main body 1 are inserted with the insertion injection core 4, and the insertion injection core 4 comprises a sealing ring 5, a liquid inlet 6, a fixing ring 7, a liquid inlet tube 8, a second mounting ring 9 and a fixing tube 10, and the sealing ring 5 is placed between the pillar tube main body 1 and the insertion injection core 4, and the center of the sealing ring 5 is penetrated and provided with the liquid inlet 6, and the liquid inlet 6 is far away from one side of the pillar tube main body 1 and is provided with the liquid inlet tube 8, and the outer diameter surface of the liquid inlet tube 8 is sleeved with the fixing ring 7, and the outer diameter surface of the fixing ring 7 is sleeved with the second mounting ring 9, and one side of the second mounting ring 9 far away from the sealing ring 5 is provided with the fixing tube 10, wherein the sealing ring 5 is made of perfluoroether rubber, and the liquid inlet tube 8 is a flow structure of the first flow guiding component 2 into the pillar tube main body 1, wherein the material of the liquid inlet tube 8 is made of titanium alloy, has corrosion resistance and biocompatibility, and the liquid inlet inner diameter of the liquid inlet tube 8 is gradually reduced to 0.5mm by 1.2mm, and then the laminar flow transition is realized, the fixing ring 7 is used for carrying out the static stress concentration on the fixing ring is reduced by the fixing ring 8, and the fixing ring is arranged on the outer surface of the fixing ring through the fixing ring, and the fixing ring is fixed ring 9, and the fixing ring is fixedly connected with the fixing ring, and the fixing ring is fixed ring is provided with the fixing ring, and the fixing ring is provided with the fixing ring device, and is provided with the fixing ring device, and with the fixing ring.
As a preferred embodiment, a liquid outlet 11 is disposed at one end of the column tube main body 1 far away from the liquid inlet 6, a liquid outlet 12 is disposed at one end of the liquid outlet 11 far away from the liquid inlet 6, a third mounting ring 13 is sleeved on the outer diameter surface of the liquid outlet 12, and the liquid inlet end and the liquid outlet end of the column tube main body 1 have the same structure.
As a preferred embodiment, a docking ring 14 is installed between the column tube main body 1 and the first diversion component 2, a detachable structure is formed between the column tube main body 1 and the first diversion component 2 through the docking ring 14, and a detachable structure is formed between the column tube main body 1 and the second diversion component 3 through the external thread wall 1010 and the internal thread wall 302.
The application has the working processes that the spectrum column tube structure is firstly provided with a column tube main body 1, a first flow guiding component 2 and a second flow guiding component 3, wherein when the column tube main body 1 structure is used, when a sample enters the interior of the column tube main body 1, a spiral flow guiding groove 102 arranged on a tube wall 101 of the inner wall of the column tube main body 1 is used, a forced flowing phase advances along a spiral path, so that a more uniform and stable flow mode is formed in the column by guiding the sample and the flowing phase, thereby improving separation performance, and a micro spoiler 107 is also arranged in the tube wall 101 to adjust the flowing property of the sample in the column tube main body 1, so that micro protrusions arranged in the column generate local turbulence to the flowing of the sample, destroy a laminar boundary layer, prevent mass transfer resistance caused by 'wall-attached flow' of the flowing phase, enable the flowing layer area of the sample in the column tube main body 1 to be changed from laminar flow to be more approximate to plug flow, reduce axial diffusion, reduce theoretical plate height, represent narrower and more symmetrical chromatographic peaks, improve resolution, reduce performance caused by column packing difference or long-term use, prolong chromatographic life, enable the flow to flow, and form a turbulent flow in the flow, and flow can flow in the column main body 1 along the spiral flow guiding groove 102 when the inner part is not formed in the column tube main body 1, and the flow guide flow main body is mixed along the spiral flow guide body when the flow main body is in a radial direction, so that the flow guiding flow is not in the flow guide area (flow guiding flow guide area is formed by the spiral flow main body 102, or flow guide area is formed in the flow main body is formed), meanwhile, the arranged micro spoiler 107 generates local turbulence on the sample in the pillar tube main body 1 after flowing, and damages a laminar boundary layer, thereby improving mass transfer efficiency.
Wherein the temperature control element 103 optimizes the separation selectivity by integrating a resistance wire at the outer wall of the column body 1, precisely controlling the column temperature, and the model of the temperature control element 103 is Custom-thermo electric-12711-5L31-03CL, and the model of the micro pressure sensor 104 is XJC-Z15-H9-2M, wherein the micro pressure sensor 104 monitors the column body 1 to monitor the first dimension chromatographic column pressure, triggers fraction collection, and feeds back pressure changes in real time, adjusts the blowback flow rate, and then wherein an optical window 105 provided in the column body 1 allows on-line detection to perform ultraviolet absorption and fluorescence detection to perform part of the sample, wherein the optical window 105 is quartz or sapphire transparent material, and the delivery chamber 106 is a space for the column body 1 to deliver the sample, such that the sample passes through the delivery chamber 106 and is guided.
And the ports at the two ends of the column tube main body 1 are provided with a first preassembly interface 108 and a second prefabricated structure, wherein the first preassembly interface 108 and the second prefabricated structure have the same structure, and the first preassembly interface 108 arranged at the water inlet end of the column tube main body 1 is used for connecting the docking ring 14, so that the column tube main body 1 is quickly connected with the docking ring 14 through the first preassembly interface 108, and the risk of dead volume and leakage is reduced.
Wherein the fixed collar 201 is a starting end of the first diversion component 2, and is directly connected with the first preassembled interface 108 of the column tube main body 1, wherein after the first diversion component 2 is connected with the column tube main body 1, mechanical fixing is provided for the column tube main body 1, and simultaneously, the coaxiality of the component and the column tube is ensured, rapid disassembly and assembly are realized between the first diversion component 2 and the column tube main body 1 through threads, and when the sample enters the column tube main body 1 through the first diversion component 2, the sample passes through the inside of the absorption ring 202, wherein the sample passes through the absorption ring 202, at the moment, the sample contacts with activated carbon in the absorption ring 202, for capturing trace impurities in the sample, and the absorption ring 202 is in a detachable design structure, for facilitating periodic replacement of the absorption ring 202, and the sample enters the inside of the pressurizing tube 203 before entering the absorption ring 202, wherein the inside diameter of the pressurizing tube 203 is in a gradual change structure, for adjusting the pressure of the sample in the process of passing through the absorption ring 202, and the pressurizing tube 203 and an external air path system are in a way, when the sample enters the column tube main body 1, the pressure control box 205 is arranged through the connection tube main body 205, and the polishing device is used for controlling the flow rate of the sample is connected with the first diversion device, and the first diversion ring 2 is connected with the external connection pipe 204, the first diversion device is provided for connecting the sample is in a flow interface 204, and the interface is connected with the first diversion device, and the interface 204 is in a way, and the mechanical interface is connected, wherein the sample flows out of the connecting pipe 205, the pressurizing pipe 203 connected with the first mounting ring 204, the sample subjected to pressure adjustment, the impurity adsorbed and then enters the fixed sleeve 201, and finally the impurity is conveyed to the downstream column main body 1 by the fixed sleeve 201, wherein the first flow guiding component 2 adopts metal sealing, and the leakage rate is less than or equal to 1 multiplied by 10 < - 9 > Pa.m3/s.
The locking ring 301 is positioned at the edge of the inner wall of the locking ring 301, wherein the specification of the thread is M6×0.75, the thread is used for being fast screwed with the external thread of the column pipe, and the locking ring 301 is directly embedded into the side wall of the locking ring 301, and by adopting an eccentric cam structure, the axial pretightening force which is more than or equal to 50N M can be generated during screwing, so that the loosening under the high-pressure working condition is prevented.
Then, when the sample enters the second flow guiding component 3 through the column tube main body 1, the sample enters the cavity of the outer connecting tube 304, wherein the outer connecting tube 304 is a transitional flow path of the second flow guiding component 3, the inner diameter of the outer connecting tube 304 is matched with the column tube, the tolerance is +/-0.01 mm, the flow speed mutation is avoided, and the connection of the mounting tube 305 and the plug tube 306 is used for connecting with equipment.
Wherein the material of the sealing ring 5 is perfluoroether rubber, and the liquid inlet pipe 8 is a flowing structure of the first diversion component 2 entering the column pipe main body 1, wherein the material of the liquid inlet pipe 8 is titanium alloy, corrosion resistance and biocompatibility are achieved, the liquid inlet inner diameter of the liquid inlet pipe 8 is gradually reduced to 0.5mm through 1.2mm to achieve laminar flow transition, then the fixing ring 7 is used for positioning the liquid inlet pipe 8, the axial position of the liquid inlet pipe 8 is fixed through interference fit, and the stress concentration of the pipe wall 101 under high pressure is reduced through annular groove design, then the second mounting ring 9 is sleeved on the outer surface of the liquid inlet pipe 8, wherein the outer surface of the second mounting ring 9 is plated with gold to prevent static accumulation, and the fixing ring 7 is used for fixing the liquid inlet pipe 8 at the end, wherein the fixing ring 7 is connected with external equipment through a clamp structure. The liquid inlet end and the liquid outlet end of the column tube main body 1 have the same structure, and the operating principle of the chromatographic column tube structure with the flow guiding structure is the above.
Claims (9)
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| CN202510238248.XA CN119959435B (en) | 2025-03-03 | 2025-03-03 | A chromatographic column tube structure provided with a flow guiding structure |
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| CN202510238248.XA CN119959435B (en) | 2025-03-03 | 2025-03-03 | A chromatographic column tube structure provided with a flow guiding structure |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN221377842U (en) * | 2023-11-30 | 2024-07-19 | 浙江月旭材料科技有限公司 | Peak-shaped front-delay inhibiting structure for liquid chromatograph |
| CN118706977A (en) * | 2024-06-21 | 2024-09-27 | 浙江月旭材料科技有限公司 | Liquid chromatography analysis device, liquid chromatography analysis method and use thereof |
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| US5059654A (en) * | 1983-02-14 | 1991-10-22 | Cuno Inc. | Affinity matrices of modified polysaccharide supports |
| CN2063597U (en) * | 1989-07-18 | 1990-10-10 | 李钟镐 | Tube with spiral flow ridge on internal surface |
| US7469557B2 (en) * | 2004-06-12 | 2008-12-30 | Teresanne Griffin | Method for forming a capillary column for filtering, separation and concentration |
| JP4690864B2 (en) * | 2005-11-11 | 2011-06-01 | 日本曹達株式会社 | Analysis method, separation method, mixer, and analyzer |
| DE102008037008B3 (en) * | 2008-08-08 | 2010-04-08 | Dionex Softron Gmbh | Mixing device for liquid chromatography |
| DE102009032394B4 (en) * | 2009-07-08 | 2013-05-02 | Dionex Softron Gmbh | A method of longitudinal mixing of a liquid comprising at least two liquid components for high performance liquid chromatography |
| US10073068B2 (en) * | 2013-09-18 | 2018-09-11 | Agilent Technologies, Inc. | Liquid chromatography columns with structured walls |
| CN104749296A (en) * | 2013-12-26 | 2015-07-01 | 江苏鑫润环保科技有限公司 | Liquid chromatographic column for analysis |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN221377842U (en) * | 2023-11-30 | 2024-07-19 | 浙江月旭材料科技有限公司 | Peak-shaped front-delay inhibiting structure for liquid chromatograph |
| CN118706977A (en) * | 2024-06-21 | 2024-09-27 | 浙江月旭材料科技有限公司 | Liquid chromatography analysis device, liquid chromatography analysis method and use thereof |
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