CN119817803A - Preparation method and application of uric acid-reducing gout-treating medicine-food homologous composition - Google Patents

Abstract

The invention discloses a preparation method and application of a medicinal and edible composition for reducing uric acid and treating gout, and belongs to the field of crossing of medicine and food technology. The composition consists of celery seed, pagodatree flower bud, chicory, dandelion, mulberry twig, papaya and fresh cogongrass, and the components act synergistically to play roles in inhibiting uric acid generation, promoting uric acid excretion, resisting inflammation, relieving swelling and regulating multiple targets of organism metabolism. The preparation method adopts the steps of intelligent screening and fine pretreatment of raw materials, multistage targeted extraction technology, intelligent concentration and precise molding, and can efficiently extract active ingredients and prepare various dosage forms. In the aspect of accurate medical assistance, personalized medication schemes are customized according to different stages of gout, whole-course digital management is realized by means of intelligent equipment and a cloud platform, and in the daily intelligent health care of families, gout is prevented conveniently at the families through intelligent household kitchen equipment and uric acid monitoring packages, and the composition is used for innovating health food development and providing a natural, efficient and multifunctional new health management way for gout patients.

Description

Preparation method and application of uric acid-reducing gout-treating medicine-food homologous composition

Technical Field

The invention relates to the field of crossing of medicine and food technology, in particular to a preparation method and application of a medicine-food homologous composition for reducing uric acid to treat gout, and particularly focuses on developing an innovative preparation method and multiple applications of the composition for reducing uric acid to treat gout based on medicine-food homologous food materials, aiming at filling the blank of natural, efficient and multifunctional products in the existing gout treatment scheme and providing a brand-new health management approach for patients with gout and potential hyperuricemia.

Background

Along with the change of life style, gout becomes a global health problem, the incidence rate of hyperuricemia rises year by year, and a plurality of pains such as joint pain, kidney injury and the like are brought to patients. Although traditional drug treatment can control uric acid level and relieve symptoms in a short period, long-term use often accompanies serious side effects such as liver and kidney function damage, gastrointestinal discomfort and the like, so that the compliance of patients to treatment is greatly reduced. Meanwhile, consumers are increasingly advocated on natural and healthy health preserving concepts, and medicinal and edible products are favored because of having medicinal effects and food safety. The celery seeds can effectively promote uric acid excretion by virtue of obvious diuretic and detumescent effects, flavonoid compounds rich in pagodatree flower buds have anti-inflammatory and antioxidant properties, can pertinently relieve gout inflammation, chicory can help reduce uric acid level by regulating organism metabolic pathways, dandelion has good effect of relieving joint inflammation and heat pain during acute onset of gout by clearing heat and detoxicating, mulberry branches are good in dredging channels and collaterals and benefiting joints, can improve joint movement disorder of gout patients, pawpaw is good for helping digestion and invigorating stomach, bioactive components contained in the pawpaw can comprehensively regulate organism, and fresh lalang grass rhizome has the effect of clearing heat and promoting urination for uric acid excretion in vitro. However, most of the medicinal and edible compositions for gout in the current market have the problems of simple and extensive preparation process, low extraction rate of active ingredients, single efficacy, limited application scene and the like, and are difficult to meet the accurate treatment requirement under the complex pathological mechanism of gout and the pursuit of people on convenience and daily health care. Therefore, development of a medicine and food homologous composition which has an innovative preparation method, a multi-target action mechanism and wide and flexible application is urgent.

Disclosure of Invention

1. Technical problem to be solved

In view of the shortcomings and drawbacks of the existing formulas and technologies, the invention provides a preparation method and application of a medicinal and edible composition for reducing uric acid and treating gout. Western medicines have a certain effect in uric acid reduction treatment, but often have obvious side effects, such as damage to liver and kidney functions, gastrointestinal discomfort (such as nausea, vomiting and diarrhea) and skin rash, and other allergic reactions. The side effects not only affect the medication compliance of patients, but also can bring other hidden dangers to the physical health of the patients in the long term, and limit the wide application and long-term use of western medicines in the uric acid reducing field. The traditional Chinese medicine decoction has the problem of inconvenient administration, needs to be decocted, consumes long time, has bitter and astringent taste and is inconvenient to carry, so that a plurality of patients are difficult to keep taking the decoction for a long time. Although the convenience of taking the Chinese patent medicine is improved, the whole Chinese patent medicine has relatively slow effect, has an obvious effect on reducing uric acid and relieving gout symptoms, always needs to be continuously taken for a long time to see a certain effect, and can not timely meet the treatment requirements of patients who need to control uric acid level and relieve acute gout attack. By designing the formula of the traditional Chinese medicine composition and carefully designing the formula of the traditional Chinese medicine composition, specific medicinal and edible products are screened, and the characteristics of relatively high safety, wide sources and easy acceptance by patients of medicinal and edible substances are utilized. The medicinal and edible components have the effects of regulating metabolism of human bodies, resisting inflammation, promoting diuresis, removing dampness and the like and reducing uric acid, are expected to play a role in cooperation after reasonable collocation, radically regulate physiological processes of organism such as uric acid metabolism and the like, and improve uric acid reducing effect. The specific medicinal and edible products are screened, and the effective components in the traditional Chinese medicine composition can be selectively extracted by adopting a water treatment distillation purification mode, so that impurities, macromolecular substances and components possibly not beneficial to human bodies are removed. The purification method is relatively mild, can keep the activity of the active ingredients to the greatest extent, and can improve the concentration of the active ingredients, so that the subsequently prepared preparation can play a better role in reducing uric acid under the condition of taking smaller dosage, and can reduce potential adverse effects caused by invalid ingredients, such as gastrointestinal tract burden and the like. The purified product is prepared into uric acid-reducing oral liquid or is concentrated and then added with excipient to prepare an oral preparation, the oral liquid is convenient to take, quick to absorb, capable of quickly entering human blood circulation to play a role, capable of affecting uric acid level in the body quickly, and beneficial to timely relieving uncomfortable symptoms caused by high uric acid. The prepared other oral preparations (such as tablets, capsules and the like) have the advantages of convenient carrying, good storage stability and the like while ensuring the efficacy, and are suitable for the use requirements of patients in different scenes. The medicine has good uric acid reducing effect, effectively solves the problems of slow effect and insignificant effect of the traditional Chinese medicine decoction and the traditional Chinese patent medicines, and provides a more effective and more convenient treatment choice for patients with hyperuricemia and gout. The traditional Chinese medicine composition is subjected to water treatment, distillation and purification, the purified product is further prepared into uric acid-reducing oral liquid or is concentrated and then added with excipients to prepare an oral preparation, and an administration test proves that the traditional Chinese medicine composition has good uric acid-reducing efficacy, and solves the problems of side effects brought by western medicines, slow effect of traditional Chinese medicine decoction and Chinese patent medicines, insignificant effect and the like.

2. Technical proposal

In order to achieve the aim of the invention, the main technical scheme adopted by the invention comprises the following steps:

In a first aspect, the invention provides a preparation method and application of a uric acid reducing and gout treating medicine-food homologous composition, wherein the preparation method comprises the following steps:

S1, compatibility of traditional Chinese medicine composition, wherein the traditional Chinese medicine composition comprises celery seed, pagodatree flower bud, chicory, dandelion, mulberry twig, papaya and fresh cogongrass rhizome.

S2, purifying and preparing the traditional Chinese medicine preparation, wherein the preparation method adopts the steps of intelligent screening and fine pretreatment of raw materials, multistage targeted extraction technology, intelligent concentration and precise forming, and can efficiently extract active ingredients and prepare various dosage forms.

S3, the traditional Chinese medicine preparation is applied, in the aspect of accurate medical assistance, personalized medicine application schemes can be customized according to different stages of gout, whole-course digital management is achieved by means of intelligent equipment and a cloud platform, and in daily intelligent health care of families, gout prevention is facilitated through intelligent household kitchen equipment and uric acid monitoring packages, and the traditional Chinese medicine preparation can be used for innovation and health food development, such as uric acid regulation energy bars, gout prevention meal replacement powder and the like.

According to the preferred embodiment of the invention, in S1, the traditional Chinese medicine composition consists of 10-15 parts by mass of celery seeds, 8-12 parts by mass of pagodatree flower buds, 12-18 parts by mass of chicory, 10-15 parts by mass of dandelion, 8-12 parts by mass of mulberry twigs, 8-12 parts by mass of papaya and 15-25 parts by mass of fresh cogongrass rhizome.

Further preferably, the Chinese medicinal composition comprises 15 parts by mass of celery seed, 10 parts by mass of pagodatree flower bud, 12 parts by mass of chicory, 10 parts by mass of dandelion, 12 parts by mass of mulberry twig, 12 parts by mass of papaya and 15 parts by mass of fresh cogongrass rhizome.

According to the preferred embodiment of the invention, in S2, raw materials are intelligently screened and finely preprocessed, namely raw materials are screened by means of a spectrum analysis technology and a biological activity detection means, organic planting is selected for batches of celery seeds, chicory, dandelion and fresh cogongrass rhizome, pesticide residues are avoided, the active ingredient content is above a specific threshold value, the impurities are removed after harvesting, the materials are dried to the water content of less than 99% at the temperature of-40 ℃ to-50 ℃ by a supercritical fluid cleaning technology, the materials are manually selected to remove flaws and worm-eaten parts by means of pagodatree flower buds, mulberry twigs and papaya, and the pagodatree flower buds and the mulberry twigs are cut into small sections of 2-3 cm and papaya is cut into slices of about 0.5 cm by means of a numerical control cutting device.

According to the preferred embodiment of the invention, in S2, the multistage targeted extraction process comprises the steps of firstly mixing pretreated celery seeds, chicory, dandelion and fresh cogongrass rhizome according to a specific proportion, putting the mixture into dynamic countercurrent extraction equipment, taking deionized water as a solvent, controlling the feed-liquid ratio to be 1-1:7, the extraction temperature to be 40-50 ℃, shortening the extraction time to 0.5 hour, ultra-filtering and purifying the extract by a ceramic membrane, then accurately extracting fat-soluble active ingredients by subcritical fluid extraction technology aiming at pagodatree flower buds, mulberry branches and papaya under the conditions of the critical temperature and the pressure of 0.6-0.9MPa, removing solvent residues by supercritical carbon dioxide extraction technology after the extraction is finished, and finally, combining the two extracts, and putting the extracts into ultrasonic extraction equipment for 30-45 minutes at the ultrasonic frequency of 40kHz-60 kHz.

According to the preferred embodiment of the invention, in S2, the intelligent concentration and the accurate molding are carried out, the mixed extracting solution after ultrasonic treatment is connected into an intelligent concentration control system, the concentrated extracting solution is accurately concentrated into thick extract with the relative density of 1.2-1.3 under the conditions of the vacuum degree of 0.08-0.1MPa and the temperature of 45-55 ℃, if the oral preparation is prepared, the proper pharmaceutical excipients are added into different dosage forms according to big data analysis and clinical feedback, the 3D printing technology is adopted when the capsule is prepared, each capsule contains 0.4-0.6g of composition dry powder, the tablet is prepared by a high-speed tablet press, each tablet contains 0.5-0.7g of composition, the prepared oral solution is added with proper amount of flavoring agent and preservative, diluted to the specified concentration and canned, if the oral preparation is used for dietary addition, the extract is converted into superfine micropowder with the granularity distribution of 1-10 mu m by adopting the low-temperature spray drying technology, and the capsule is hermetically packaged.

According to the preferred embodiment of the invention, in S3, the application of the medicinal and edible composition for treating gout in accurate medical assistance is that during the acute episode of gout, the uric acid level, inflammation index and pain degree of a patient are monitored by means of intelligent medical equipment, a high-dose concentrated oral liquid treatment scheme is customized for the patient through big data analysis, the medicinal composition is taken once every 4 hours and 5ml of medicinal composition is used for tracking the rehabilitation process of the patient in real time, the dosage and the frequency of medicinal composition are timely adjusted, the medicinal composition is converted into the medicinal composition for taking intelligent controlled release capsules or sustained release tablets for maintenance treatment in intermittent and chronic periods, the release curve of the medicament in the body is set according to the individual metabolic characteristics of the patient, the uric acid level is stabilized through long-term regular administration, the recurrence of gout is prevented, the damage of urate crystals to joints and kidneys is reduced, meanwhile, a patient health management cloud platform is established, and information such as the medicinal data, the medicinal records, the movement condition and uric acid monitoring value of the patient is uploaded in real time, and the doctor provides accurate life style advice and guidance for the patient by means of artificial intelligence algorithm analysis data.

According to the preferred embodiment of the invention, in S3, the application of the medicinal and edible composition for reducing uric acid in the daily intelligent health care of families is that matched intelligent household kitchen equipment is developed, a gout diet recommendation algorithm is built in, when families prepare for cooking, users input health information of family members, the equipment automatically recommends personalized menus containing the composition, 3-8g of dry powder of the composition is accurately prompted to be added during soup cooking, 2-3 times per week is prompted to be sprayed during porridge cooking, 2-5g of dry powder is ensured to be fully dissolved and reserved in the cooking process through an intelligent cooking program, a family uric acid monitoring set is pushed out, the intelligent cooking process comprises a portable uric acid detector, a mobile phone APP and the like, family members can periodically and automatically detect uric acid level, the APP uploads detection data to a cloud platform, the diet intake data is combined, the trend of uric acid fluctuation is analyzed by using a machine learning model, the user is timely warned of high uric acid risk, the users are reminded to adjust the structure, and the application frequency of the composition in diet is increased.

According to the preferred embodiment of the invention, in S3, the application of the uric acid-reducing and gout-treating medicine-food homologous composition in deep development of innovative health foods is that a series of high-tech and functional foods are developed by utilizing the composition based on a human nutrition demand model and a gout control target, such as developing uric acid regulation energy bars, a 3D printing food forming technology is adopted, the composition dry powder is precisely combined with raw materials such as high-quality protein, dietary fiber, low-sugar carbohydrate and the like, each energy bar contains 5g of the composition dry powder, the dual requirements of quick energy supplementation and uric acid regulation of sports lovers before and after exercise are met, gout prevention meal replacement powder is developed, and components such as probiotics and probiotics are added in combination with an intestinal microorganism targeting regulation technology, so that the meal replacement powder can provide a satiety and simultaneously optimize intestinal flora, promote uric acid excretion, and a convenient and healthy gout prevention solution is provided for busy office workers.

3. Advantageous effects

(1) Pharmacological efficacy

The uric acid reducing effect is achieved through multi-component synergistic adjustment, wherein celery seeds contain apigenin and other components, pagodatree flower buds are rich in rutin and other flavonoid substances, chicoric acid and other compounds, dandelion is provided with dandelion sterol and other components, morin in mulberry branches, oleanolic acid contained in pawpaw and various saccharides of fresh cogongrass rhizome are synergistic from different angles, key enzyme activity in the uric acid generating process can be inhibited, uric acid production can be reduced, uric acid excretion can be promoted, uric acid level in a human body can be maintained in a normal range through bidirectional adjustment through increasing the functions of filtering and excreting uric acid by kidneys, therefore blood uric acid concentration is effectively reduced, hyperuricemia is relieved, and gout attack is prevented.

The combination of the medicinal and edible traditional Chinese medicinal materials is beneficial to regulating the physiological processes related to purine metabolism, water metabolism and the like of the whole human body, radically improves the condition of uric acid metabolic disturbance, is not only purely symptomatic relief, but also can realize a more durable and stable uric acid reducing effect.

The composition has the effects of resisting inflammation, detumescence and pain, wherein inflammation manifestations such as red swelling, heat and pain occur to joints when gout occurs, and various components in the composition have anti-inflammatory activity, for example, flavonoid substances can inhibit the release of inflammatory mediators, relieve local inflammatory reaction of joints, relieve pain and swelling symptoms, reduce the formation of tophus, improve joint functions and improve the life quality of gout patients.

Repairing damaged tissue, namely, the joint and surrounding tissues are easily damaged by long-term gout attack, and some components (such as nutrition components contained in ramulus mori and the like) in the composition can be helpful for promoting the repair and regeneration of damaged articular cartilage, synovium and other tissues, delaying the further development of joint lesions and protecting the health of the joint.

(2) Safety aspect

The medicinal and edible characteristics ensure that the selected medicinal and edible materials are higher in safety compared with some chemical synthetic medicines or some traditional Chinese medicines with certain toxicity, the medicinal and edible materials generally cannot cause obvious damage to important organs such as liver and kidney of a human body under normal use dosage, cannot easily cause serious adverse reactions, are suitable for long-term administration to maintain stable uric acid level and control gout diseases, and are higher in acceptance and compliance of patients.

The natural medicinal and edible components are relatively mild, so that the effects of reducing uric acid and treating gout are exerted, and meanwhile, the common side effects such as gastrointestinal discomfort (such as nausea, vomiting and diarrhea), influence on hematopoiesis, skin rash and the like are basically avoided as those of certain western medicines, and the body pain and the additional health risk caused by the side effects of the administration are reduced.

(3) Convenience and applicability of administration

The anti-hyperuricemia nursing agent is widely applicable to people with gout, can be used for patients with gout to help the patients to relieve symptoms and control disease development, is also applicable to people with hyperuricemia, and can be used as a preventive conditioning means for regulating uric acid metabolism to prevent uric acid from further rising and developing into gout. And the people with different ages and different constitutions can endure the medicinal and edible composition mostly as long as the medicinal indications are met, and the application range is wider.

The preparation has various and convenient administration forms, can be prepared into various dosage forms according to different preparation methods, such as oral liquid, is convenient to take, quick to absorb and suitable for patients with normal swallowing function, can be prepared into tablets and capsules, is convenient to carry and store, can meet the administration requirements of people in different scenes such as daily travel, can be prepared into powder, and the like, can flexibly adjust the administration dosage according to the self condition, is convenient for the patients to select proper dosage forms to take according to the self preference and actual condition, and improves the compliance of administration.

(4) In the aspect of economic cost

The raw materials have the advantages that most of the raw materials of celery seed, pagodatree flower bud, chicory, dandelion, mulberry twig, papaya and fresh cogongrass rhizome are wide in sources, the yield is relatively considerable in nature, the purchasing cost is much lower than that of some rare and precious traditional Chinese medicinal materials or imported uric acid reducing medicines, the production cost of the whole composition is reduced, the final product has higher competitive power in price, more patients can be affordable, the popularization and the application of the product are facilitated, and more hyperuricemia and gout patients benefit.

Drawings

FIG. 1 is a process flow diagram of a method for preparing a medicinal and edible composition for reducing uric acid and treating gout.

Figure 2 is a 3D perspective pie chart of the ratio of the ingredients of the edible and pharmaceutical composition of the present invention.

Fig. 3 is a diagram of the external appearance of the oral preparation made by the invention.

Figure 4 is a graph of a nutritional porridge according to the invention in the food state after dietary addition.

Fig. 5 is a diagram of a smart kitchen device diet collocation interface.

Detailed Description

The invention will be better explained by the following detailed description of the embodiments with reference to the drawings.

The invention discloses a preparation method and application of a medicinal and edible composition for reducing uric acid and treating gout, and mainly characterized by disclosing a preparation method and application of a medicinal and edible composition for reducing uric acid and treating gout, belonging to the field of crossing of medicine and food technology. The composition consists of celery seed, pagodatree flower bud, chicory, dandelion, mulberry twig, papaya and fresh cogongrass rhizome according to specific parts by weight, and the components act synergistically to play roles in inhibiting uric acid generation, promoting uric acid excretion, resisting inflammation, reducing swelling, regulating organism metabolism and other multiple targets. The preparation method adopts the steps of intelligent screening and fine pretreatment of raw materials, multistage targeted extraction technology, intelligent concentration and precise molding, and can efficiently extract active ingredients and prepare various dosage forms. In the aspect of accurate medical assistance, the composition can customize personalized medication schemes according to different stages of gout, realizes whole-course digital management by virtue of intelligent equipment and a cloud platform, is convenient for families to prevent gout by intelligent household kitchen equipment and uric acid monitoring suite in daily intelligent health care of families, can be used for innovating health food development such as uric acid regulation energy bars, gout prevention meal replacement powder and the like, provides a natural, efficient and multifunctional new health management way for gout patients and hyperuricemia crowds, and has good curative effect and safety through animal experiments, human clinical experiments and safety experiments.

Wherein the Chinese medicinal composition comprises semen Apii Graveolentis, flos Sophorae Immaturus, herba Cichorii, herba Taraxaci, ramulus Mori, fructus Chaenomelis, and fresh lalang grass rhizome.

Preferably, the traditional Chinese medicine composition comprises 10-15 parts by weight of celery seeds, 8-12 parts by weight of pagodatree flower buds, 12-18 parts by weight of chicory, 10-15 parts by weight of dandelion, 8-12 parts by weight of mulberry twigs, 8-12 parts by weight of papaya and 15-25 parts by weight of fresh cogongrass rhizome. Further preferably, the Chinese medicinal composition comprises 15 parts by mass of celery seed, 10 parts by mass of pagodatree flower bud, 12 parts by mass of chicory, 10 parts by mass of dandelion, 12 parts by mass of mulberry twig, 12 parts by mass of papaya and 15 parts by mass of fresh cogongrass rhizome.

In the traditional Chinese medicine composition, the functions of the medicinal materials are as follows:

1. Celery seed contains various active ingredients, wherein flavonoid compounds such as apigenin, apigenin A and the like have remarkable anti-oxidative stress capability, can relieve oxidative damage caused by high uric acid in vivo, and protect cells from free radical attack. Meanwhile, the butylphthalide substance contained in the composition has the effects of dilating blood vessels and improving microcirculation, which is beneficial to promoting the blood circulation of excretory organs such as kidneys and the like, and creates a good hemodynamic environment for uric acid excretion, thereby enhancing the uric acid excretion efficiency.

2. The pagodatree flower bud is rich in flavonoid substances such as rutin, quercetin and the like, and is a key component for playing a pharmacological role. On one hand, the flavonoid components can inhibit activation and chemotaxis of inflammatory cells, reduce release of local inflammatory mediators such as interleukin-1 beta, tumor necrosis factor-alpha and the like of joints during acute onset of gout, and effectively relieve inflammation symptoms such as red swelling, hot pain and the like of the joints, and on the other hand, the flavonoid components can also indirectly influence uric acid metabolism by regulating lipid metabolism in vivo, reducing blood lipid level, because the hyperlipoidemia is often associated with hyperuricemia, improve blood lipid and help to restore uric acid homeostasis.

3. Chicory contains rich components such as chicoric acid, chicory polysaccharide and the like. Chicoric acid has the effect of regulating the activity of a key enzyme for purine metabolism, such as xanthine oxidase, which is a key rate-limiting enzyme in the process of uric acid production, and inhibiting the activity of the enzyme can reduce uric acid production from the source. The chicory polysaccharide can regulate the intestinal flora structure, promote the proliferation of beneficial bacteria, improve the barrier function of the intestinal tract, further influence the absorption and metabolism of the body on purine, reduce the conversion of exogenous purine into uric acid, and realize the comprehensive regulation and control of uric acid level.

4. The dandelion comprises dandelion sterol, dandelionin and the like which are active components of dandelion, has strong heat-clearing and detoxifying effects, and can quickly relieve the red swelling and pain symptoms of joints when gout is at the beginning of the process, and the main reason is the anti-inflammatory and detumescence action mechanism of the dandelion. It can inhibit the conduction of inflammatory signal pathway, reduce capillary permeability, reduce inflammatory exudation, promote blood circulation of local tissue, accelerate the removal of metabolic waste including urate crystals, and relieve inflammatory injury of joint.

5. Ramulus Mori Sang Zhifu contains components such as Phellinus linteus and alkaloid, and has effects of dredging channels and collaterals and benefiting articulation in Chinese medicine theory. From the modern medical point of view, the mulberry twig extract can regulate the functions of joint synovial cells, inhibit the excessive proliferation and inflammatory reaction of the synovial cells, reduce effusion in joints, improve the internal environment of the joints, create favorable conditions for the dissolution and discharge of urate crystals, and simultaneously relieve the movement disorder of gout patients caused by joint lesions.

6. Papaya contains bioactive components such as papain, oleanolic acid and the like. Papain helps to decompose proteins in food, promote digestion and absorption, reduce accumulation of food purine in vivo due to dyspepsia, and control uric acid increase risk from dietary source. Oleanolic acid has liver protecting effect, and can relieve liver injury possibly accompanied with hyperuricemia, maintain liver normal metabolism function, and ensure normal synthesis and secretion of uric acid synthesis and excretion related enzyme.

7. The main active ingredients of the fresh cogongrass rhizome and the fresh cogongrass rhizome comprise imperatorin, arundoin and the like, and have obvious heat clearing and diuretic properties. By increasing the blood flow of the kidney, the glomerular filtration rate is promoted, so that urine generation is increased, sufficient carriers are provided for uric acid excretion, uric acid is accelerated to be excreted out of the body along with urine, and uric acid level in the body is directly reduced, so that the composition is a key assistance for uric acid excretion links in the composition.

The synergistic principle is that in the composition, all the components are mutually synergistic to form an organic whole for regulating uric acid metabolism and gout prevention and treatment in all directions. The celery seed and the fresh cogongrass rhizome cooperate to strengthen the uric acid excretion passage, the former improves the blood circulation of excretory organs, and the latter directly promotes urine generation to promote uric acid to be discharged; the Chinese scholartree flower bud and dandelion are combined to resist gout inflammation, relieve acute attack symptoms, inhibit inflammatory medium release, relieve joint injury, chicory regulates purine metabolism from the source, reduces uric acid generation, acts together with papain for controlling dietary purine absorption, controls uric acid yield under double-tube condition, and the mulberry twig is concentrated to improve intra-articular environment, is beneficial to urate treatment, and is matched with other components regulated integrally, so that gout condition improvement is comprehensively ensured, and in the process of exerting efficacy of each component, the liver protection effect of the papaya is that liver metabolism supports navigation, the stable operation of an enzyme system related to uric acid metabolism is ensured, and finally, the excellent effects of reducing uric acid efficiently, treating gout and having high safety are realized.

The preparation method adopts the steps of intelligent screening and fine pretreatment of raw materials, multistage targeted extraction process, intelligent concentration and precise molding, and can efficiently extract active ingredients and prepare various dosage forms. The purification process can release the Chinese medicine active ingredients from the cell walls, improve the medicine effect, and the purification process also decomposes the Chinese medicine macromolecular substances into small molecular substances, so that the small molecular substances are easier to combine with human proteins and transport into human cells, are fully metabolized in the body, avoid medicine residues, improve the bioavailability, reduce the medicine effect and the utilization rate of the organism to the medicine in low dosage, reduce the using amount of the Chinese medicine, reduce the toxic and side effects of the Chinese medicine and improve the acting speed.

The invention relates to a medicinal and edible composition for reducing uric acid and treating gout, which comprises the following preparation method:

1. The intelligent screening and fine pretreatment of the raw materials, namely screening out the raw materials with high quality and high activity by means of advanced spectrum analysis technology and biological activity detection means. For celery seed, chicory, dandelion and fresh cogongrass rhizome, the batches which are planted organically, have no pesticide residue and have the active ingredient content above a specific threshold value are preferably selected. After harvesting, immediately adopting a supercritical fluid cleaning technology, rapidly removing impurities in a low-temperature and pollution-free environment, then adopting a vacuum freeze drying technology, drying the materials at-40 ℃ to-50 ℃ until the water content is lower than 99% at-40 ℃ to-50 ℃, and maximally retaining the thermosensitive active ingredients. The pagodatree flower bud, the mulberry twig and the papaya are strictly selected manually, flaws and worm-eaten parts are removed, then the pagodatree flower bud and the mulberry twig are cut into small sections of 2-3 cm according to the accurate size requirement by using numerical control cutting equipment, and the papaya is cut into thin slices of about 0.5 cm, so that the subsequent extraction uniformity and the subsequent extraction efficiency are ensured.

2. The multistage targeted extraction technology is a multistage targeted extraction technology of firstly fusing dynamic countercurrent extraction, subcritical fluid extraction and ultrasonic auxiliary extraction. Firstly, mixing the pretreated celery seeds, chicory, dandelion and fresh cogongrass rhizome according to a specific proportion, putting the mixture into dynamic countercurrent extraction equipment, taking deionized water as a solvent, controlling the feed-liquid ratio to be 1:5-1:7, extracting at 40-50 ℃, realizing continuous and efficient water-soluble component extraction through countercurrent circulation, shortening the extraction time to 0.5 hour, improving the efficiency by 80% compared with the traditional water extraction process, ultrafiltering and purifying the extract by a ceramic membrane, removing macromolecular impurities, and retaining high-purity water-soluble active ingredients.

Then, aiming at pagodatree flower bud, mulberry twig and papaya, subcritical fluid (such as butane, propane and the like) extraction technology is adopted, and under the conditions that the critical temperature is below and the pressure is 0.6-0.9MPa, fat-soluble active ingredients are accurately extracted, and the technology can effectively avoid the damage of high temperature to heat-sensitive ingredients, and the extraction rate is improved by 80 percent compared with that of the traditional alcohol extraction technology. After the extraction is finished, the solvent residue is rapidly removed by using a supercritical carbon dioxide extraction technology, so that the purity and safety of the extract are ensured.

3. Finally, combining the two extracts, placing into an ultrasonic extraction device, performing ultrasonic treatment at 40kHz-60kHz for 30-45 minutes, further breaking plant cells by means of ultrasonic cavitation effect, releasing deep-layer combined state components, and improving the comprehensive activity of the extracts.

And (3) intelligent concentration and accurate molding, namely, the mixed extract after ultrasonic treatment is connected into an intelligent concentration control system, and is accurately concentrated into thick extract with the relative density of 1.2-1.3 under the conditions of the vacuum degree of 0.08-0.1MPa and the temperature of 45-55 ℃ according to parameters such as component concentration, density and the like monitored in real time. If the oral preparation is prepared, proper pharmaceutical excipients are added according to big data analysis and clinical feedback aiming at different dosage forms. For example, when preparing capsules, the 3D printing technology is used, the extract, microcrystalline cellulose, magnesium stearate and other auxiliary materials are prepared into individualized capsule shells and filling dry powder according to the optimal human body absorption model, each capsule contains 0.4-0.6g of composition dry powder, a high-speed tablet press is used for preparing tablets, the auxiliary materials such as lactose, starch, disintegrating agent and the like are mixed by combining a drug release dynamics principle, the tablets are pressed into tablets, each tablet contains 0.5-0.7g of composition, stable and efficient release of the drugs in the body is ensured, oral liquid is prepared, and based on a taste optimization algorithm, a proper amount of flavoring agent and preservative are added, and the oral liquid is diluted to a specified concentration and canned. If the extract is used for dietary supplement, the extract is converted into superfine powder by adopting a low-temperature spray drying technology, the granularity is distributed between 1 and 10 mu m, and the extract is packaged in a sealing way for convenient use by consumers.

The invention relates to a medicinal and edible composition for reducing uric acid and treating gout, which is applied to the following steps:

1. omnibearing expansion of multi-element application scenes:

1.1, accurate medical assistance:

In the acute onset stage of gout, the uric acid level, inflammation index and pain degree of a patient are monitored by means of intelligent medical equipment, a high-dose concentrated oral liquid treatment scheme is customized for the patient through big data analysis, and the concentrated oral liquid is taken once every 4 hours, 5ml of the concentrated oral liquid is taken once every time, so that uric acid is rapidly diminishing inflammation and relieving pain, uric acid is reduced, and joint severe pain is relieved. Meanwhile, the wearable equipment is utilized to track the rehabilitation progress of the patient in real time, and the dosage and frequency of the medicine are adjusted in time. In the intermittent period and the chronic period, the method is converted into the maintenance treatment of taking intelligent controlled-release capsules or sustained-release tablets, the release curve of the medicine in the body is set according to the individual metabolic characteristics of patients, and the uric acid level is stabilized, the gout recurrence is prevented, and the damage of urate crystals to joints and kidneys is reduced through long-term regular administration.

The method comprises the steps of establishing a patient health management cloud platform, uploading information such as patient medication data, diet records, exercise conditions, uric acid monitoring values and the like in real time, analyzing the data by a doctor through an artificial intelligence algorithm, providing accurate life style advice and medication guidance for the patient, and realizing whole-course digital management of gout treatment.

1.2, Daily intelligent health care of families:

Developing matched intelligent household kitchen equipment and internally arranging a gout diet recommendation algorithm. When the household is ready for cooking, the user only needs to input household member health information, the device automatically recommends a personalized menu containing the composition, for example, when soup is cooked, 3-8g of dry powder of the composition is accurately prompted to be added according to food matching and nutrition balancing principles, the composition ingredients are fully dissolved and reserved in the cooking process through an intelligent cooking program, the flavor of dishes can be added, and the families can silently regulate uric acid metabolism in daily diet. When the porridge is cooked, the equipment also intelligently guides to scatter 2-5g of dry powder, and is matched with food materials such as vegetables, lean meat and the like, so that the porridge has balanced nutrition and provides a mild prevention means for people at risk of high uric acid.

The household uric acid monitoring set is pushed out, and comprises a portable uric acid detector, a mobile phone APP and the like, so that family members can periodically and automatically detect uric acid level, the APP uploads detection data to a cloud platform, dietary intake data are combined, a machine learning model is used for analyzing uric acid fluctuation trend, high uric acid risk is early warned in time, a user is reminded of adjusting a dietary structure, the application frequency of a composition in diet is increased, and household health autonomous management is realized.

2. Deep development of innovative health food:

The composition is used for developing a series of high-tech and functional foods based on a human nutrition demand model and gout prevention and treatment targets in cooperation with food science and technology enterprises. If developing uric acid regulation energy bars, adopting a 3D printing food forming technology, accurately combining the composition dry powder with high-quality protein, dietary fiber, low-sugar carbohydrate and other raw materials, wherein each energy bar contains 5g of the composition dry powder, so as to meet the dual requirements of rapid energy supplement and uric acid regulation of sports lovers before and after exercise, develop gout prevention meal replacement powder, combine intestinal microorganism targeted regulation technology, add prebiotics, probiotics and other components, ensure that the meal replacement powder can provide satiety and simultaneously optimize intestinal flora, promote uric acid excretion, and provide a convenient and healthy gout prevention solution for busy office workers.

The following are preferred embodiments of the present invention.

Step1, preparing uric acid reducing oral liquid

(1) Weighing 15 parts by weight of celery seeds, 10 parts by weight of pagodatree flower bud, 12 parts by weight of chicory, 10 parts by weight of dandelion, 12 parts by weight of mulberry twig, 12 parts by weight of papaya, and 15 parts by weight of fresh cogongrass rhizome

(2) The active ingredients can be extracted efficiently and prepared into oral preparations by intelligent screening and fine pretreatment, multistage targeted extraction process, intelligent concentration and accurate molding steps of the traditional Chinese medicine raw materials.

Experimental design and data

Animal experiment:

The aim of the experiment is to evaluate the uric acid reducing and anti-inflammatory effects of the composition on animal models of hyperuricemia.

The experimental animals are selected from 60 male SD rats, the weight of the experimental animals is 200-220g, and the experimental animals are randomly divided into 6 groups of 10 animals each.

The modeling method is to construct a hyperuricemia model by injecting potassium oxazinate (300 mg/kg) into the abdominal cavity once a day for 7 days.

After successful molding, the control group is given with equal amount of physiological saline, the low dose group is given with 100mg/kg of the composition, the medium dose group is given with 200mg/kg of the composition, the high dose group is given with 400mg/kg of the composition, and the positive control group is given with allopurinol (10 mg/kg), and the stomach is irrigated once a day for 14 days.

The detection index comprises collecting rat blood after administration, detecting serum uric acid, creatinine and urea nitrogen level, collecting joint synovial tissue, and detecting expression level of inflammatory factors TNF-alpha and IL-6.

As a result of the experiment, compared with a control group, serum uric acid levels of the composition groups are obviously reduced (16.3% in a low-dose group, 33.6% in a medium-dose group, 45.7% in a high-dose group and 39.3% in an IL-6-dose group), uric acid reducing effects of the composition groups are equivalent to those of a positive control group, and TNF-alpha and IL-6 expression levels in joint synovial tissues are obviously reduced (15.4% in the low-dose group, 19.3% in the IL-6 group, 34.9% in the medium-dose group, 26.7% in the IL-6 group, 46.8% in the TNF-alpha in the high-dose group and 39.3% in the IL-6 group, and P < 0.05) in each dose group of the composition, so that the composition has good anti-inflammatory effect. The specific data are shown in Table 1.

Group of	Serum uric acid reduction rate (%)	TNF-alpha reduction rate (%)	IL-6 reduction Rate (%)
				Low dose group	16.3	15.4	19.3
Medium dose group	33.6	34.9	26.7
				High dose group	45.7	46.8	39.3
Positive control group	46.3	44.6	42.3

TABLE 1

Human clinical trial:

The experimental purpose is to verify the clinical curative effect and safety of the composition in gout patients.

Subjects were enrolled in 100 patients with confirmed gout, aged 35-65 years, randomized into experimental and control groups of 50 persons each.

The treatment scheme is that the traditional Chinese medicine preparation capsules prepared by the composition are taken by patients in an experimental group every day, 3 capsules are taken every time, and the placebo capsules are taken by patients in a control group, wherein the dosage of the placebo capsules is the same. The treatment course is 3 months.

The detection index comprises detecting serum uric acid, blood sedimentation and C-reactive protein level of the patient each month, recording the times of joint pain and pain degree of the patient (adopting visual simulation scoring method, VAS), and observing the change of liver and kidney function index of the patient.

Experimental results after treatment, serum uric acid levels of patients in the experimental group were reduced by 120. Mu. Mol/L on average compared with those before treatment, and the difference was significant compared with the control group (P <

0.05 Blood sedimentation, C reactive protein level is also obviously reduced (blood sedimentation is reduced by 15mm/h, C reactive protein is reduced by 8mg/L, P is less than 0.05), the number of times of arthralgia attack is reduced by 3 times/month, VAS score is averagely reduced by 2.5 minutes, and liver and kidney function indexes have no obvious abnormal change, so that the composition has good safety when effectively treating gout. The specific data are shown in Table 2.

TABLE 2

Safety acute toxicity test:

The experimental animals are 20 healthy Kunming mice, and the weight is 18-22g, and the male and female animals are half.

Administration method the composition extract was administered at the maximum tolerated dose (20 g/kg) and the mice were observed for general status, diet, drinking water, excretion and death after a single administration for 14 consecutive days.

The experimental result shows that the mice have no obvious toxic reaction after being dosed, normal diet and drinking water, no death phenomenon, smooth fur, free movement, bright eyes, no secretion and normal fecal form and color. The composition extract has extremely low acute toxicity, basically has no damage to organisms at extremely large dosage, and preliminarily verifies the edible safety. Specific observations are detailed in table 3.

TABLE 3 Table 3

Long-term toxicity test:

the experimental animals are selected from 80 SD rats, each half of which has a weight of 150-180g, and are randomly divided into 4 groups of 20 animals each.

Dosing regimen control group was given an equivalent amount of physiological saline, low dose group was given 2g/kg of composition extract, medium dose group was given 5g/kg, high dose group was given 10g/kg, and the administration was performed once daily by gavage for 90 days.

The detection index comprises recording rat weight, food intake and water intake every week, collecting blood for detecting blood convention every half month, and collecting liver and kidney function index (glutamic pyruvic transaminase, glutamic oxaloacetic transaminase, creatinine, urea nitrogen, etc.), and collecting main viscera (heart, liver, spleen, lung, kidney, stomach, intestine, etc.) at the end of test

A pathological histological examination was performed.

The experimental results show that the weight growth trend of rats in each dosage group is similar to that of rats in a control group in the whole experimental period, the weight growth trend of rats in each dosage group has no obvious difference (P is more than 0.05), the food intake and water intake of rats in each dosage group have no obvious fluctuation, the blood routine indexes are all in a normal range, the difference of the liver and kidney function indexes in each dosage group has no statistical significance (P is more than 0.05) compared with the control group, and the pathological tissue examination shows that the tissue structure of each viscera is clear, and the abnormal conditions such as obvious lesions, inflammations, cell necrosis and the like are avoided. The combination shows that the composition has no obvious toxic and side effect on organisms and has good long-term edible safety under long-term and large-dose administration. The detailed data are shown in tables 4-7.

TABLE 4 weight changes (units: g, mean.+ -. Standard deviation) in rats for each dose group

Group of	Food intake at week 1	Food intake at week 2	Food intake at week 3	......
					Control group	20±2	22±3	23±2	......
Low dose group	19±2	21±3	22±2	......
					Medium dose group	21±2	23±3	24±2	......
High dose group	20±3	22±3	23±3	......

TABLE 5 variation of rat feeding amount (unit: g/day, mean.+ -. Standard deviation) for each dose group

TABLE 6 liver and kidney function index changes (unit: U/L or. Mu. Mol/L or nmol/L, mean.+ -. Standard deviation) for rats in each dose group

TABLE 7 summary of the results of the pathological histological examination of the various organs

The comprehensive safety test fully proves that the medicinal and edible composition of the invention has high safety on organisms in short-time and oversized dose contact or long-time regular administration from two dimensions of acute and long-time, and lays a solid foundation for the subsequent wide application in the fields of medical treatment, health care and food.

It should be noted that the above embodiments are merely for illustrating the technical solution of the present invention and not for limiting the same, and although the present invention has been described in detail with reference to the above embodiments, it should be understood by those skilled in the art that the technical solution described in the above embodiments may be modified or some or all of the technical features may be equivalently replaced, and these modifications or substitutions do not make the essence of the corresponding technical solution deviate from the scope of the technical solution of the embodiments of the present invention.

Claims (8)

1. A preparation method and application of a medicinal and edible composition for reducing uric acid and treating gout are characterized in that the medicinal and edible composition comprises

S1, compatibility of traditional Chinese medicine composition, wherein the traditional Chinese medicine composition comprises celery seed, pagodatree flower bud, chicory, dandelion, mulberry twig, papaya and fresh cogongrass rhizome.

S2, purifying and preparing the traditional Chinese medicine preparation, wherein the preparation method adopts the steps of intelligent screening and fine pretreatment of raw materials, multistage targeted extraction technology, intelligent concentration and precise forming, and can efficiently extract active ingredients and prepare various dosage forms.

S3, the traditional Chinese medicine preparation is applied, in the aspect of accurate medical assistance, personalized medicine application schemes can be customized according to different stages of gout, whole-course digital management is achieved by means of intelligent equipment and a cloud platform, and in daily intelligent health care of families, gout prevention is facilitated through intelligent household kitchen equipment and uric acid monitoring packages, and the traditional Chinese medicine preparation can be used for innovation and health food development, such as uric acid regulation energy bars, gout prevention meal replacement powder and the like.

In the S1, the traditional Chinese medicine composition consists of 10-15 parts by weight of celery seeds, 8-12 parts by weight of pagodatree flower buds, 12-18 parts by weight of chicory, 10-15 parts by weight of dandelion, 8-12 parts by weight of mulberry twigs, 8-12 parts by weight of papaya and 15-25 parts by weight of fresh cogongrass rhizome.

2. The oral preparation and the preparation method thereof according to claim 1, wherein the Chinese medicinal composition comprises 15 parts by mass of celery seeds, 10 parts by mass of pagodatree flower buds, 12 parts by mass of chicory, 10 parts by mass of dandelion, 12 parts by mass of mulberry twigs, 12 parts by mass of papaya and 15 parts by mass of fresh cogongrass rhizome.

3. The oral preparation and the preparation method thereof according to claim 1 are characterized in that S2, raw materials are intelligently screened and finely preprocessed, wherein the raw materials are screened by means of a spectrum analysis technology and a biological activity detection means, batches which are planted organically, have no pesticide residue and have active ingredient content above a specific threshold value are selected, the impurities are removed by a supercritical fluid cleaning technology after harvesting, the materials are dried to the water content of less than 99% under the condition of-40 ℃ to-50 ℃ by a vacuum freeze drying technology, and after flaws and worm-eaten parts of pagodatree flower buds, mulberry twigs and papaya are manually selected and removed, the pagodatree flower buds and the mulberry twigs are cut into small sections of 2 cm to 3 cm and papaya is cut into slices of about 0.5 cm by a numerical control cutting device.

4. The oral preparation and the preparation method thereof according to claim 1 are characterized in that in S2, the multistage targeted extraction process comprises the steps of firstly mixing pretreated celery seeds, chicory, dandelion and fresh cogongrass rhizome according to a specific proportion, putting into dynamic countercurrent extraction equipment, taking deionized water as a solvent, controlling the feed liquid ratio to be 1:7, the extraction temperature to be 40-50 ℃, shortening the extraction time to 0.5 hour, ultra-filtering and purifying the extract by a ceramic membrane, then accurately extracting fat-soluble active ingredients for pagodatree flower buds, mulberry branches and papaya under the conditions of critical temperature and pressure of 0.6-0.9MPa by adopting subcritical fluid extraction technology, removing solvent residues by adopting supercritical carbon dioxide extraction technology after extraction, and finally merging the two extracts, and putting into ultrasonic extraction equipment for 30-45 minutes at the ultrasonic frequency of 40-60 kHz.

5. The oral preparation and the preparation method thereof according to claim 1 are characterized in that the intelligent concentration and the accurate molding in S2 are carried out, the mixed extract after ultrasonic treatment is connected into an intelligent concentration control system, the concentrated extract is accurately concentrated to thick extract with the relative density of 1.2-1.3 under the conditions of the vacuum degree of 0.08-0.1MPa and the temperature of 45-55 ℃, if the oral preparation is prepared, proper pharmaceutical excipients are added for different dosage forms according to big data analysis and clinical feedback, the 3D printing technology is adopted when capsules are prepared, each capsule contains 0.4-0.6g of composition dry powder, a high-speed tablet press is used for preparing tablets, each tablet contains 0.5-0.7g of composition, a proper amount of flavoring agent and preservative are added into the prepared oral liquid, the oral liquid is diluted to a specified concentration tank, and if the oral liquid is used for dietary addition, the extract is converted into superfine micropowder with the granularity distribution of 1-10 mu m by adopting a low-temperature spray drying technology, and the sealing packaging is carried out.

6. The oral preparation and the preparation method thereof according to claim 1 are characterized in that in S3, the application of the uric acid treatment gout medicine-food homologous composition in accurate medical assistance is that in the acute episode of gout, the uric acid level, inflammation index and pain degree of a patient are monitored by means of intelligent medical equipment, a high-dose concentrated oral liquid treatment scheme is customized for the patient by big data analysis, the oral preparation is taken once every 4 hours, the rehabilitation progress of the patient is tracked in real time by using a wearable device every 5ml, the medicine dosage and frequency are timely adjusted, the oral preparation is converted into the administration of intelligent controlled release capsules or slow release tablets for maintenance treatment in intermittent and chronic periods, the release curve of the medicine in the body is set according to the individual metabolic characteristics of the patient, the medicine is taken regularly for stabilizing the uric acid level, preventing the recurrence of gout, reducing the damage of urate crystallization to joints and kidneys, meanwhile, a patient health management cloud platform is established, the medicine data, diet records, movement conditions, uric acid monitoring values and other information of the patient are uploaded in real time, and doctors provide accurate life style advice and medicine guidance for the patient by means of artificial intelligent algorithm analysis data.

7. The oral preparation and the preparation method thereof according to claim 1 are characterized in that in S3, the application of the gout-reducing medicine-food homologous composition in daily intelligent health care of families is that a matched intelligent household kitchen device is developed, a gout diet recommendation algorithm is built in, when families are ready to cook, a user inputs family member health information, the device automatically recommends a personalized recipe containing the composition, 3-8g of dry powder of the composition is accurately prompted to be added in soup cooking, 2-3 times per week is prompted to be scattered in porridge cooking, 2-5g of dry powder is prompted to be scattered in porridge cooking, the composition ingredients are ensured to be fully dissolved and reserved in the cooking process through an intelligent cooking program, a family uric acid monitoring set is pushed out, the family uric acid monitoring set comprises a portable uric acid detector, a mobile phone APP and the like, the family members can automatically detect uric acid level, the APP uploads detected data to a cloud platform, and combines diet intake data, a machine learning model is used for analyzing uric acid fluctuation trend, a user is timely warned to adjust a diet structure, and the application frequency of the composition in diet is increased.

8. The oral preparation and the preparation method thereof according to claim 1 are characterized in that S3, the application of the uric acid reducing and treating gout medicine and food homologous composition in the deep development of innovative health foods is that the composition is utilized to develop a series of high-tech and functional foods based on a human nutrition demand model and a gout prevention and treatment target, such as developing uric acid regulating energy bars, adopting a 3D printing food forming technology, accurately combining dry powder of the composition with raw materials such as high-quality protein, dietary fiber, low-sugar carbohydrate and the like, each energy bar contains 5g of dry powder of the composition, thereby meeting the dual requirements of rapidly supplementing energy and regulating uric acid before and after exercise of sports lovers, developing gout prevention meal replacement powder, combining intestinal microorganism targeting regulation technology, adding components such as prebiotics and probiotics, optimizing intestinal flora while providing satiety, promoting uric acid excretion, and providing a convenient and healthy gout prevention solution for busy office workers.