CN119534881A

CN119534881A - Sample analysis system and method

Info

Publication number: CN119534881A
Application number: CN202411602199.5A
Authority: CN
Inventors: 罗强; 郭静
Original assignee: Maccura Medical Electronics Co Ltd
Current assignee: Maccura Medical Electronics Co Ltd
Priority date: 2024-11-11
Filing date: 2024-11-11
Publication date: 2025-02-28

Abstract

The application provides a sample analysis system and a sample analysis method. The sample analysis system comprises a first sample analyzer for detecting a first type of detection item, a second sample analyzer for detecting a second detection item, and a sample transport device, wherein the second type of detection item comprises an ion detection item, when the information of the items to be detected of the sample of the target sample tube comprises the first type of detection item and the second detection item, a carrying unit carrying the target sample tube is transported to the first sample analyzer and the second sample analyzer through the sample transport device, and before a first sampling needle of the first sample analyzer absorbs the sample from the target sample tube, a cleaning module of the first sample analyzer is used for cleaning the ground sampling needle sequentially by adopting cleaning liquid and deionized water. The method avoids the influence of the cleaning liquid of the first sample analyzer on the detection result of the second sample analyzer, reduces the cross contamination between the analyzers, and improves the overall detection accuracy of the sample analysis system.

Description

Sample analysis system and method

Technical Field

The application relates to the field of sample analysis, in particular to a sample analysis system and a sample analysis method.

Background

With the rapid development of medical technology, sample analyzers are becoming increasingly widely used as the primary devices for medical diagnosis. In order to improve the efficiency of sample detection and perfect the test flow, a plurality of sample analyzers of different types can be spliced and cascaded into a sample analysis system. However, when the sample analysis system is used to detect the same target sample tube, cross contamination may occur, thereby affecting the detection accuracy of the sample analysis system. Therefore, how to reduce cross contamination between different sample analyzers in a sample analyzer system and improve the detection accuracy of the sample analyzer system has become a problem to be solved.

Disclosure of Invention

The application provides a sample analysis system and a sample analysis method, and various aspects related to the embodiments of the application are described below.

In a first aspect, a sample analysis system is provided, which comprises a first sample analyzer, a second sample analyzer and a sample transport device, wherein the first sample analyzer comprises a first sampling needle and a first cleaning module, the first sample analyzer is used for detecting a first type of detection item on a sample sucked by the first sampling needle, the first cleaning module is used for cleaning the first sampling needle, the second sample analyzer comprises a second sampling needle and is used for detecting a second type of detection item on the sample sucked by the second sampling needle, the second type of detection item is different from the first type of detection item, the second type of detection item comprises an ion detection item, the sample transport device is used for transporting a sample carrying unit with a sample tube to the first sample analyzer or/and the second sample analyzer, the sample transport device is used for transporting a sample carrying unit with the sample tube to be detected to the first sample analyzer and the second sample analyzer when information of the items to be detected of the sample tube comprises the first type of detection item and the second type of detection item, and the sample transport the sample carrying unit with the sample tube to the sample carrying unit is used for carrying the sample tube to the first sample analyzer and the second sample analyzer, the sample carrying unit is used for cleaning the sample tube to sequentially and the sample tube to be cleaned before the sample is sucked by the first sample tube and the sample analyzer is cleaned by the first sample analyzer.

In a second aspect, the method is applied to a sample analysis system, the sample analysis system comprises a first sample analyzer and a first cleaning module, the first sample analyzer is used for detecting a first type of detection item of a sample sucked by the first sample analyzer, the first cleaning module is used for cleaning the first sample analyzer, the second sample analyzer comprises a second sample analyzer, the second sample analyzer is used for detecting a second type of detection item of the sample sucked by the second sample analyzer, the second type of detection item is different from the first type of detection item, the second type of detection item comprises an ion detection item, a sample transporting device is used for transporting a sample carrying unit with a sample tube to the first sample analyzer or/and the second sample analyzer, the sample tube is provided with a sample, the method comprises the steps of determining information of items to be detected of a target sample tube, responding to the information of items to be detected of the target sample tube comprises the first type of detection item and the first detection item, the sample carrying device is used for carrying the sample to the first sample analyzer and the first sample analyzer in a sample carrying mode, and the sample carrying unit is used for cleaning the sample tube with the first sample carrying unit and the first sample tube in a sample carrying mode of the first sample analyzer.

The sample analysis system provided by the embodiment of the application can be used online by a first sample analyzer and a second sample analyzer, wherein the first sample analyzer is used for detecting a first type of detection item, the second sample analyzer is used for detecting a second type of detection item, the second type of detection item comprises an ion detection item, when the information of the items to be detected of the sample of the target sample tube of the sample analysis system comprises the first type of detection item and the second type of detection item, a carrying unit carrying the target sample tube is carried to the first sample analyzer and the second sample analyzer through a sample carrying device, and before a first sampling needle of the first sample analyzer absorbs the sample from the target sample tube, a cleaning module of the first sample analyzer is used for cleaning the first sampling needle by sequentially adopting cleaning liquid and deionized water. The influence of the cleaning liquid of the first sample analyzer on the detection result of the second sample analyzer is avoided, and the cross contamination between the first sample analyzer and the second sample analyzer is reduced, so that the overall detection accuracy of the sample analysis system is improved.

Drawings

In order to more clearly illustrate the technical solution of the embodiments of the present application, the drawings that are required to be used in the embodiments will be briefly described.

It is appreciated that the following drawings depict only certain embodiments of the application and are not to be considered limiting of its scope.

It should also be understood that the same or similar reference numerals are used throughout the drawings to designate the same or similar elements.

It should also be understood that the drawings are merely schematic and that the dimensions and proportions of the elements in the drawings are not necessarily accurate.

Fig. 1 is a schematic structural diagram of a sample analysis system according to an embodiment of the present application.

Fig. 2 is a schematic structural diagram of a first sample analyzer according to an embodiment of the present application.

Fig. 3 is a schematic structural diagram of a second sample analyzer according to an embodiment of the present application.

Fig. 4 is a schematic structural diagram of a cleaning tank according to an embodiment of the present application.

Fig. 5 is a schematic view of the structure taken along A-A in fig. 4.

Fig. 6 is a schematic view of a structure taken along B-B in fig. 4 in section.

Fig. 7 is a schematic view of a structure sectioned along C-C in fig. 4.

Fig. 8 is a schematic diagram of a part of a liquid path structure of a first sample analyzer according to an embodiment of the present application.

Fig. 9 is a flow chart of a sample analysis method according to an embodiment of the present application.

Reference numerals sample analysis system 100, first sample analyzer 110, first sampling needle 111, first washing module 112, washing cell 113, deionized water injection port 114, first needle washing chamber 115, second needle washing chamber 116, air port 117, first air port 1171 and second air port 1172, reaction cup buffer tray 118, first detection module 119, first gripper 141, second gripper 142, third gripper 143, reagent bottle 144, first incubation tray 145, first reagent dispensing arm 146, second reagent dispensing arm 147, first magnetic separation module 148, second magnetic separation module 149, air pump 151, overflow valve 152, positive pressure actuator 153, air blowing line 154, negative pressure actuator 155, pump 156, deionized water line 157, deionized water tank 158, second sample analyzer 120, second sampling needle 121, second incubation tray 122, second detection module 123, cuvette 124, reagent cartridge 125, reagent cartridge 126, mixing module 127, third reagent dispensing arm and second washing module 129, transport module 128, sample loading and transport module 132, sample recovery tube 133, sample loading unit 132, sample loading tube 133.

Detailed Description

Embodiments of the present application are described below by way of example with reference to the accompanying drawings. It should be understood that the present application may be embodied in many different forms and should not be construed as limited to the embodiments set forth herein, but rather should be construed in a more thorough and clear understanding of the present application.

With the rapid development of medical technology, sample analyzers are becoming increasingly widely used as the primary devices for medical diagnosis. Sample analyzers may also be referred to as in vitro diagnostic devices, and may be used to detect samples of blood (whole blood, plasma or serum), urine, body fluids or tissues. For example, the sample analyzer may be a blood cell analyzer, a biochemical analyzer, an immunoassay analyzer, a urine analyzer, a coagulation analyzer, or the like. The categories of items detected by the sample analyzers of different categories are different.

In order to improve the efficiency of sample detection and perfect the test flow, a plurality of sample analyzers of different types can be spliced and cascaded into a sample analysis system (or sample analysis assembly line) so as to realize the detection of a plurality of types of detection items on some samples to be detected at the same time. For example, the sample analysis system may be a biochemical immunoassay system, a blood analysis system, or the like. In some cases, the sample to be detected may be a sample in a different sample tube, i.e. at least two sample analyzers in the sample analysis system draw samples from different sample tubes, respectively. In other cases, the sample to be detected may be a sample in one sample tube, i.e. at least two sample analyzers in a sample analysis system need to aspirate samples from the same sample tube in succession. By using at least two sample analyzers to detect samples in the same sample tube, multiple tubes of samples from the same patient can be avoided and patient pain can be reduced to a large extent.

In general, a sample analyzer uses a sampling needle to draw a sample in a sample tube and discharges the sample into a cuvette or a reaction cell inside the sample analyzer for detection. In order to prevent the sample attached to the sampling needle from affecting the detection result of the next sample, the sampling needle is cleaned after completing the sample discharge every time.

For the sample analysis system described above, each sample analyzer has its own individual sampling needle and cleaning module. Different sample analyzers all use respective sampling needles to sample, and after each sample arrangement is completed, the sampling needles are cleaned by respective cleaning modules. In order to meet the requirement of carrying pollution rate, part of sample analyzers need to clean the sampling needle by adopting cleaning liquid.

The inventor of the present application has found that, when at least two sample analyzers in a sample analysis system need to detect samples in the same sample tube (hereinafter, simply referred to as online in-tube detection), if one of the sample analyzers (i.e., the first sample analyzer) uses a cleaning solution to clean the sampling needle, and the detection items of the other sample analyzer (i.e., the second sample analyzer) include ion detection items, then the ion item detection result of the second sample analyzer has an inaccurate problem. The cleaning solution used by the first sample analyzer contains ion components, the ion concentration of the cleaning solution is high, and after the sampling needle is cleaned by the cleaning solution, the cleaning solution attached to the sampling needle of the first sample analyzer is mixed into the sample tube, so that the deviation of the ion item detection result of the second sample analyzer exceeds an acceptable range, thereby affecting the ion item detection result of the second sample analyzer and causing cross contamination of the instrument. For example, the sample tube is firstly transported to the first sample analyzer for absorbing samples, then transported to the second sample analyzer for absorbing samples, the cleaning liquid attached to the sampling needle of the first sample analyzer is mixed into the sample tube to influence the initial detection result of the ion item of the samples by the second sample analyzer, or the sample tube is firstly transported to the second sample analyzer for absorbing samples and then transported to the first sample analyzer for absorbing samples, if the second sample analyzer is required to absorb samples again from the sample tube for re-measurement, the cleaning liquid mixed in the sample tube can influence the re-measurement result of the ion item of the samples by the second sample analyzer, so that the deviation of the initial or re-measurement result of the second sample analyzer exceeds the acceptable range, and cross contamination among instruments in the sample analysis system is caused.

One possible implementation way to address the above problem is to set the sampling needle of the first sample analyzer to use a disposable TIP head, thereby avoiding the cleaning process, i.e. avoiding the influence of the cleaning liquid. However, this approach is not only environmentally friendly but also costly. Another possible implementation is to avoid using multiple sample detectors to detect the same tube of sample, i.e. multiple tubes of sample are taken from the same patient, and then several sample analyzers on-line detect the respective sample tubes separately, which however increases the pain of the patient. As yet another possible implementation, a preprocessing module may be added to the sample analysis system, where the preprocessing module may divide the same sample tube into two sample tubes, so that the subsequent situation that different sample analyzers detect the same sample tube is avoided, where the preprocessing module for sample division needs to be added, which increases both the cost of the sample analysis system and the complexity of the workflow of the sample analysis system.

In summary, in online in-line detection, how to avoid the influence of the cleaning solution of the first sampling needle of the first sample analyzer on the detection result of the second sample analyzer including the ion detection item while reducing the cost is a technical problem to be solved.

In view of the above, the embodiment of the application provides a sample analysis system, which comprises a first sample analyzer, a second sample analyzer and a sample transportation device, wherein the first sample analyzer comprises a first sampling needle and a first cleaning module, the first sample analyzer is used for detecting a first type of detection item on a sample sucked by the first sampling needle, the first cleaning module is used for cleaning the first sampling needle, the second sample analyzer comprises a second sampling needle, the second sample analyzer is used for detecting a second type of detection item on the sample sucked by the second sampling needle, the second type of detection item is different from the first type of detection item, the second type of detection item comprises an ion detection item, the sample transportation device is used for transporting a sample carrying unit with a sample tube to the first sample analyzer or/and the second sample analyzer, the sample transportation device is used for carrying the carrying unit with the sample tube to be detected to the first sample analyzer and the second sample analyzer when the information of the sample to be detected item of the target sample tube comprises the first type of detection item and the second type of detection item, and the sample transportation device is used for cleaning the sample carrying unit with the first sample analyzer and the second sample analyzer before the first sample tube is sucked by the first sampling needle and the first sample analyzer is used for cleaning the sample tube sequentially by the cleaning module. The method avoids the mixing of the cleaning liquid into the target sample tube, thereby avoiding the influence of the cleaning liquid for cleaning the first sampling needle on the ion item detection result of the second sample analyzer when the first sample analyzer and the second sample analyzer are used for detecting samples in the same target sample tube, reducing the cross contamination between the sample analyzers, improving the detection precision of a sample analysis system, and simultaneously, the method has lower cost.

A sample analysis system 100 in accordance with an embodiment of the present application is described in detail below with reference to fig. 1-8. As shown in fig. 1, a sample analysis system 100 in an embodiment of the present application includes a first sample analyzer 110, a second sample analyzer 120, and a sample transport 130.

As shown in fig. 2, the first sample analyzer 110 includes a first sampling needle 111, a first washing module 112, a first detection module 119, and a first control module (not shown in fig. 2). The first sampling needle 111 may also be referred to as a sample dispensing arm for drawing a sample from the sample tube 134. The first cleaning module 112 is used for cleaning the first sampling needle 111. In the embodiment of the present application, the first cleaning module 112 has a cleaning function and a deionized water cleaning function, and the first cleaning module 112 may clean the first sampling needle 111 with the cleaning solution and/or deionized water before the first sampling needle 111 sucks the sample. The first control module is used for controlling the first sampling needle 111, the first cleaning module 112 and the first detection module 119.

The wash solution employed by the first sample analyzer 110 typically includes sodium chloride and/or potassium chloride and/or a phosphate buffer system. As one implementation, the phosphate buffer system is one of a disodium hydrogen phosphate-monopotassium phosphate system, a sodium dihydrogen phosphate-dipotassium hydrogen phosphate and a dipotassium hydrogen phosphate-monopotassium phosphate buffer system. Based on this, the cleaning solution used by the first sample analyzer 110 typically contains ionic components. The ionic components thereof may include, but are not limited to, one or more of potassium ion, sodium ion, chloride ion, phosphate ion, hydrogen phosphate ion, and dihydrogen phosphate ion.

The first detection module 119 is configured to detect a first type of detection item on the sample sucked by the first sampling needle 111.

As one implementation, the first sample analyzer 110 may be a hemagglutination analyzer. Blood coagulation analyzers, also known as coagulation analyzers, are used to assess the clotting functions of blood to aid in the diagnosis and monitoring of hemorrhagic diseases, thrombophilia, and other coagulation-related diseases. In this case, the first type of detection item is a detection item related to the coagulation function of blood.

As another implementation, the first sample analyzer 110 may be an immunoassay analyzer. The immunoassay analyzer may also be referred to as a chemiluminescent immunoassay analyzer (or a chemiluminescent analyzer). Immunoassay devices are used to qualitatively or quantitatively detect analytes in serum, plasma, urine, cerebrospinal fluid and the like. At this time, the first type of detection items include hormones, tumor-associated antigens, infectious diseases, allergen-associated items, and the like.

As shown in fig. 3, the second sample analyzer 120 includes a second sampling needle 121, a second detection module 123, and a second control module (not shown in fig. 2). The second sampling needle 121 is used to aspirate a sample from the sample tube 134. The second detection module 123 is configured to detect a second type of detection item on the sample sucked by the second sampling needle 121. Wherein the second sample analyzer 120 is different from the first sample analyzer 110, the second type of detection items are different from the first type of detection items and the second type of detection items include ion detection items. That is, the second sample analyzer 120 is a sample analyzer that can perform ion detection. The second control module is used for controlling the second sampling needle 121 and the second detection module 123 and the second cleaning module 129 hereinafter.

As one implementation, the second sample analyzer 120 is a biochemical analyzer or an electrolyte analyzer or a biochemical analyzer with electrolyte analysis module. The second sample analyzer 120 is used to detect electrolyte levels and/or clinical biochemical indicators of the sample.

As shown in fig. 1, the sample transport device 130 includes a sample recovery module 131 and a transfer rail 132. The sample recovery module 131 is used for placing the sample carrying unit 133 for transferring to the transfer rail 132 and recovering the sample carrying unit 133 from the transfer rail 132. The transport rail 132 is used for transporting the sample carrying unit 133. The number of sample carrying units 133 may be at least one, and at least one sample tube 134 may be carried in a single sample carrying unit 133, with a sample in the sample tube 134. The sample carrying unit 133 may be a sample rack. In embodiments of the present application, the sample in sample tube 134 may be serum, plasma, or urine. The sample transport 130 is used to transport the sample carrying unit 133 with the sample tube 134 placed therein to the first sample analyzer 110 or/and the second sample analyzer 120.

In an embodiment of the present application, the sample analysis system 100 further includes a third control module (not shown in FIG. 2) communicatively coupled to the sample transporter 130. When the item information to be detected of the target sample tube X includes the first type of detection item and the second type of detection item, the third control module is used to control the sample transportation device 130 to transport the sample carrying unit 133, on which the target sample tube X is placed, to the first sample analyzer 110 and the second sample analyzer 120.

The target sample tube X is one of the plurality of sample tubes 134 carried by the sample carrying unit 133, and the tag on the target sample tube X may indicate that the item information to be detected of the target sample tube X includes both the first type of detection item and the second type of detection item. As an implementation manner, the sample analysis system 100 further includes an information acquisition module, where the information acquisition module is configured to identify a tag of the target sample tube X, so as to acquire information of a to-be-detected item of the target sample tube X.

When the information of the items to be detected of the target sample tube X includes a first type of detection item and a second type of detection item, the first control module is configured to control the first cleaning module 112 to sequentially clean the first sampling needle 111 with the cleaning solution and the deionized water before the first sampling needle 111 sucks the sample in the target sample tube X. That is, when the item information to be detected of the target sample tube X includes a first type of detection item and a second type of detection item, the first control module is configured to control the first cleaning module 112 to clean the first sampling needle 111 with the cleaning liquid and then clean the first sampling needle 111 with deionized water before the first sampling needle 111 sucks the sample in the target sample tube X.

The sample analysis system 100 provided by the embodiment of the application can realize the online operation of the first sample analyzer 110 and the second sample analyzer 120 to detect the samples in the same-tube target sample tube X, and can also use the first cleaning module 112 to firstly clean the first sampling needle 111 of the first sample analyzer 110 by adopting the cleaning liquid during online detection of the same-tube, so that the carrying pollution rate of the first sampling needle 111 meets the requirement, and then clean the first sampling needle 111 of the first sample analyzer 110 by adopting the deionized water, so that the cleaning liquid in the first sample analyzer 110 can be prevented from being mixed into the target sample tube X, thereby avoiding the cleaning liquid cleaning the first sampling needle 111 from influencing the detection result when the second sample analyzer 120 detects the samples in the target sample tube X, reducing the cross contamination among the sample analysis system instruments, and improving the detection precision of the sample analysis system 100.

The embodiment of the application does not specifically limit specific detection items included in the ion detection items, as long as the detection items include detection of ion components. Specifically, the ion detection items include one or more of potassium, sodium, chlorine, calcium, phosphorus, magnesium, and the like ions.

In view of this, in some embodiments, when the item information to be detected of the target sample tube X includes a first type of detection item and an ion detection item, and the ion detection item includes one or more of potassium ion, sodium ion, chloride ion, calcium ion, magnesium ion, and phosphorus ion detection item, the first cleaning module 112 is configured to sequentially clean the first sampling needle 111 with a cleaning solution and deionized water before the first sampling needle 111 sucks the sample in the target sample tube X.

The inventors have further studied and found that although the ion components in the cleaning liquid employed by the first sample analyzer 110 may affect the detection result of the ion detection item in the second sample analyzer 120, the influence of different ion components in the cleaning liquid on the detection result of the ion detection item is different. For example, when the ion detection items are sodium ions and/or chloride ions, the cleaning liquid used by the first sample analyzer 110 may affect the result, but the effect may be acceptable, because the content of sodium ions and chloride ions in the sample (i.e., the sample in the target sample tube X) is high. For another example, when the ion detection item is potassium ion and/or phosphorus ion, since the content of potassium ion and/or phosphorus ion in the sample is low, the potassium ion and/or phosphorus ion in the cleaning solution used by the first sample analyzer 110 may have a prominent effect on the detection result of potassium ion and/or phosphorus ion by the second sample analyzer 120, which is not acceptable.

In view of this, in some embodiments, when the item information to be detected of the target sample tube X includes a first type of detection item and an ion detection item, and the ion detection item includes a potassium ion or/and a phosphorus ion detection item, the first cleaning module 112 is configured to sequentially clean the first sampling needle 111 with a cleaning solution and deionized water before the first sampling needle 111 sucks the sample in the target sample tube X, so that deionized water can be saved and cost can be reduced.

Preferably, in some embodiments, when the first sample analyzer 110 is the immunoassay analyzer shown in fig. 2, and the immunoassay analyzer is connected to the second sample analyzer 120 capable of performing ion detection to detect the sample of the same-tube target sample tube X, the sample transporting device 130 is configured to sequentially transport the sample carrying unit 133 with the target sample tube X placed thereon to the first sample analyzer 110 and the second sample analyzer 120. That is, when the item information to be detected of the target sample tube X includes a first type of detection item and a second type of detection item and the first sample analyzer 110 is an immunoassay analyzer, the sample transporting device 130 will first transport the sample carrying unit 133 with the target sample tube X placed thereon to the immunoassay analyzer to sample the first sample needle 111 for detection of the first type of detection item, and then the sample transporting device 130 will transport the carrying unit to the second sample analyzer 120 to sample the second sample needle 121 for detection of the second type of detection item.

With this arrangement, the problem of cross-contamination in an online scenario of the immunoassay analyzer and the second sample analyzer 120 can be reduced. This is because, although the immunoassay analyzer and the second sample analyzer 120 wash the first sampling needle 111 and the second sampling needle 121 based on the respective washing modules (i.e., the first washing module 112 and the second washing module 129) when the sample is sucked from the target sample tube X, the carrier contamination rate of the immunoassay analyzer is required to be higher than that of the second sample analyzer 120. If the target sample tube X is transported to the second sample analyzer 120 during online in-tube detection, the requirement of the second sample analyzer 120 for the carrying pollution rate of the immunoassay analyzer cannot be met, and the carrying pollution rate of the target sample tube X is increased, so that the cross pollution between the two instruments is serious, and the detection result of the immunoassay analyzer is not accurate enough. Therefore, when the item information to be detected of the target sample tube X includes the first type of detection item and the second type of detection item and the first sample analyzer 110 is an immunoassay analyzer, the sample transport device 130 is configured to sequentially transport the sample carrying unit 133 with the target sample tube X placed thereon to the first sample analyzer 110 and the second sample analyzer 120, so that the problem of cross contamination in an online scenario of the immunoassay analyzer and the second sample analyzer 120 can be reduced.

In some embodiments, when the item information to be detected of the target sample tube X includes a first type of detection item, and the first type of detection item is a plurality of items, the first sampling needle 111 is used to aspirate the sample in the target sample tube X a plurality of times. Between the first sampling needle 111 sucking up the sample in the target sample tube X a plurality of times, the first control module is for controlling the first cleaning module 112 for cleaning the first sampling needle 111 with deionized water only. For example, when the first sample analyzer 110 is an immunoassay analyzer, if item information to be detected of a sample in the target sample tube X includes 8 pieces of immunoassay before operation, the first sampling needle 111 is required to aspirate 8 samples from the target sample tube X.

By cleaning the first sampling needle 111 with deionized water only between the first sampling needle 111 sucking the samples in the target sample tube X a plurality of times when the items to be detected of the target sample tube X include a plurality of items of the first type of detection items, it is possible to save the cleaning liquid and improve the working efficiency of the first sample analyzer 110 while not affecting the detection result of the first sample analyzer 110.

In some embodiments, when the item information to be detected of the target sample tube X includes only the first type of detection items, the first control module may control the first washing module 112 to wash the first sampling needle 111 with only the washing liquid before the first sampling needle 111 draws the sample from the target sample tube X. With this arrangement, the operation efficiency of the first sample analyzer 110 can be improved while not affecting the detection result of the first sample analyzer 110.

The structure of the first cleaning module 112 is not particularly limited in the embodiment of the present application, as long as the structure of the first cleaning module 112 enables the first cleaning module 112 to have a cleaning function of a cleaning liquid and a deionized water cleaning function.

As one implementation, the first cleaning module 112 includes a cleaning liquid supply assembly (not shown) and a cleaning reservoir 113, the cleaning liquid supply assembly being in communication with the first sampling needle 111 to provide cleaning liquid to the first sampling needle 111. The cleaning liquid supply assembly may include a cleaning pipe, a cleaning liquid storage tank, a pipe control valve, a cleaning liquid driving mechanism for supplying the cleaning liquid in the cleaning liquid storage tank to the first sampling needle 111 through the cleaning pipe, etc., and the cleaning liquid supply assembly may be used to supply the cleaning liquid to the first sampling needle 111 located in the cleaning tank 113, and may clean the inner and outer walls of the first sampling needle 111. The cleaning tank 113 is provided with a deionized water injection port 114, and the first cleaning module 112 can inject deionized water into the cleaning tank 113 through the deionized water injection port 114 to clean the first sampling needle 111 positioned in the cleaning tank 113.

The structure of the cleaning tank 113 is not particularly limited in the embodiment of the present application, and as an implementation, as shown in fig. 4 to 7, the cleaning tank 113 includes a first needle washing chamber 115 and a second needle washing chamber 116. The first needle wash chamber 115 is located within the second needle wash chamber 116, and the first needle wash chamber 115 is configured to contain a cleaning solution provided by a cleaning solution supply assembly. The sidewall of the second needle washing chamber 116 is provided with a deionized water injection port 114, and the deionized water injection port 114 is used for delivering deionized water to the first sampling needle 111 coming out of the first needle washing chamber 115 so as to wash the cleaning solution on the outer wall of the first sampling needle 111.

For the above-mentioned on-line in-line detection scenario, the first cleaning module 112 may first start the cleaning function of the cleaning solution, and the first sample analyzer 110 controls the first sampling needle 111 to move to the first needle cleaning chamber 115 and then perform the first cleaning based on the cleaning solution. After the first cleaning is completed, the first sample analyzer 110 controls the first sampling needle 111 to move outwardly from the outlet of the first needle washing chamber 115 (i.e., to lift from the upper opening of the first needle washing chamber 115 shown in fig. 7) until the first sampling needle 111 is completely separated from the cleaning solution in the first needle washing chamber 115, and the first cleaning module 112 may turn on the deionized water cleaning function to control the deionized water to be supplied from the deionized water injection port 114 to the first sampling needle 111 located in the second needle washing chamber 116 for the second cleaning.

To avoid hanging liquid on the first sampling needle 111 from affecting the sampling accuracy of the first sampling needle 111, in some embodiments, the first cleaning module 112 also has a blowing function. Based on this, as shown in fig. 4 to 6, the cleaning tank 113 is further provided with an air-blowing port 117, and the air-blowing port 117 is used to blow air onto the outer wall of the first sampling needle 111 where cleaning is completed.

As an implementation manner, as shown in fig. 5 to 6, the air blowing port 117 is located on a side wall of the second needle washing chamber 116, and the air blowing channel is used for blowing air onto the outer wall of the first sampling needle 111 located in the second needle washing chamber 116, so as to reduce hanging liquid on the outer wall of the first sampling needle 111 and improve sampling precision of the first sampling needle 111. Specifically, the first cleaning module 112 may control the air blowing port 117 to blow air onto the outer wall of the first sampling needle 111 after the first sampling needle 111 completes the second cleaning.

Alternatively, as shown in fig. 4 and 5, the air blowing port 117 extends at an angle obliquely downward. Specifically, the air outlets 117 include a first air outlet 1171 and a second air outlet 1172, and the first air outlet 1171 and the second air outlet 1172 are symmetrically distributed at a certain included angle on the side wall of the second needle washing chamber 116. By symmetrically arranging two air blowing ports 117 on the side wall of the second needle washing chamber 116, air can be blown to the outer wall of the first sampling needle 111 from multiple angles, and the existence of blowing dead angles is avoided, so that the hanging liquid on the outer wall of the first sampling needle 111 is further reduced.

Alternatively, as shown in fig. 6, the deionized water injection port 114 may be located below the air blowing port 117, thereby ensuring that the first sampling needle 111 may be simultaneously deionized water rinsed and wall-blown while being located in the second needle washing chamber 116.

As one implementation way, the cleaning method for the first sampling needle 111 in the online same-tube test scene by using the cleaning pool 113 shown in FIG. 4 can specifically comprise moving the first sampling needle 111 to the first needle cleaning chamber 115, starting the cleaning liquid supply assembly to supply the cleaning liquid to the first sampling needle 111, enabling the cleaning liquid to be injected into the first needle cleaning chamber 115 through the first sampling needle 111, immersing the front end of the first sampling needle 111 into the first needle cleaning chamber 115, cleaning both the inner wall and the outer wall of the first sampling needle 111 in the first needle cleaning chamber 115, after cleaning the cleaning liquid, controlling the first sampling needle 111 to quickly and vertically lift a distance from the first needle cleaning chamber 115 to a first position, then starting the deionized water cleaning function, simultaneously horizontally moving the first sampling needle 111 from the first position to a second position, starting to be rinsed by deionized water at the second position, then controlling the first sampling needle 111 to uniformly move a distance along the vertical direction from the second position, in this movement stage, enabling the front end of the first sampling needle 111 to be immersed in the first needle cleaning chamber to clean the inner wall of the first sampling needle 111, after the cleaning liquid is blown off the first needle 117 is reset to the first needle blowing-off region, and controlling the first sampling needle 117 to be quickly and vertically moved to the needle blowing-off region. Note that, the movement trace of the first sampling needle 111 may be a trace as shown by a dotted line in fig. 7. The cleaning method can achieve the design purpose and avoid the increase of the needle washing time due to the addition of the blowing function.

In some embodiments, the first sampling needle 111 that completes the cleaning is configured to pass through the blowing region of the blowing port 117 by moving vertically, and when the needle tip of the first sampling needle 111 is about to leave the blowing region, the first sampling needle 111 is configured to draw in air to form an air segment within the first sampling needle 111. Specifically, when the needle tip of the first sampling needle 111 is located at the intersection of the axis of the first sampling needle 111 and the axis of the blowing port 117, the needle tip of the first sampling needle 111 is considered to be about to leave the blowing region.

This is because, during the blowing process, the gas blows the liquid from the outer wall of the first sampling needle 111 down to the needle tip, and the liquid falls off due to gravity, but there is still a point where the liquid is located in a semicircular structure at the needle tip (the liquid is hung on the needle tip) and cannot fall off. After the needle tip of the first sampling needle 111 leaves the blowing area, the air may be disturbed, so that the hanging liquid of the needle tip moves upwards to the outer wall of the first sampling needle 111, thereby affecting the subsequent sampling precision, so that the first sampling needle 111 needs to suck back air to form an air section in the stage of blowing to be finished, the hanging liquid of the needle tip of the first sampling needle 111 can be reduced, and the blowing effect is improved.

The embodiment of the application does not specifically limit the gas source for supplying the gas to the gas blowing port in the first sample analyzer. As an implementation, a gas source may be added independently, dedicated to supplying gas from the gas-blowing port to the second needle-washing chamber.

As another implementation, the air source may be an air pressure supply in the multiplexed first sample analyzer, thereby reducing costs as compared to independently adding an air source, while being simple and reliable in structure, and not requiring additional electronic control.

As an example, as shown in fig. 8, the first sample analyzer 110 further includes an air pump 151, an overflow valve 152, and a positive pressure actuator 153. The air pump 151 communicates with the positive pressure actuator 153 through the relief valve 152 and the air pump 151 is used to provide positive pressure to the positive pressure actuator 153. The air pump 151 may be, for example, a vacuum pump. The positive pressure actuator 153 may be, for example, a pneumatic jaw with a pneumatic cylinder, or the like. The overflow end of the overflow valve 152 may be connected to an air blowing port of the washing tub through an air blowing line, and the overflow valve 152 is used to supply the overflowed air to the washing tub 113 through an air blowing line 154. Relief valve 152 is used to maintain a steady pressure in the positive pressure line. Optionally, the air pump 151 may also be in communication with the negative pressure actuator 155 to provide negative pressure to the negative pressure actuator 155. The negative pressure actuator 155 may be, for example, a magnetic separation pump.

The structure for supplying deionized water to the deionized water injection port in the first sample analyzer according to the embodiment of the present application is not particularly limited, and as an implementation manner, as shown in fig. 8, the structure for supplying deionized water includes a pump 156, a deionized water pipe 157 and a deionized water tank 158, wherein deionized water is contained in the deionized water tank 158, and the deionized water pipe 157 connects the pump 156 with the deionized water tank 158 and the cleaning tank, respectively, so that deionized water in the deionized water tank 158 is supplied from the deionized water injection port to the cleaning tank of the first sample analyzer through the pump 156.

The structure and the detection method of the immunity analyzer are not particularly limited, and the immunity analyzer can also comprise other structures besides the first sampling needle and the cleaning module.

As an example, as shown in fig. 2, the immunoassay analyzer 110 may include a cuvette buffer tray 118, a first gripper 141, a second gripper 142, a third gripper 143, a reagent tray, a first incubation tray 145, a first reagent dispensing arm 146, a second reagent dispensing arm 147, a first magnetic separation module 148, and a second magnetic separation module 149, in addition to the first sampling needle 111, the first washing module 112, the first detection module 119, and the first control module. The first control module is used for controlling the mechanisms to work.

The working flow of the immunoassay analyzer comprises that a first sampling needle 111 (sample needle) sucks samples in a sample tube 134 on a sample carrying unit 133, the samples are discharged into a disposable empty reaction cup on a buffer disk 118, a first reagent dispensing arm 146 sucks first reagents from a reagent bottle 144 of the reagent disk and discharges the first reagents into a reaction cup, a first gripper 141 transfers reaction cups filled with the samples and the first reagents to a hole site of a first incubation disk 145, the first incubation disk 145 rotates to drive the reaction cup to a second gripper 142 grabbing position, the second gripper 142 grabs the reaction cup to a first magnetic separation module 148 to clean the reaction cup for the first time, the second gripper 142 grabs the reaction cup which completes the first cleaning to a second reagent filling position Y, the second reagent dispensing arm 147 sucks second reagents from a reagent bottle 144 of the reagent disk and discharges the second reagents to the reaction cup, the second gripper 142 grabs the reaction cup back to the hole site of the first incubation disk 145, the first gripper 145 rotates to drive the reaction cup to a third gripper 143, the third gripper rotates to drive the reaction cup to the hole site of the third incubation disk 145, the third gripper 149 rotates to drive the reaction cup to the first magnetic separation module to clean the reaction cup 141 for the first time, and the first incubation disk is detected to detect the first magnetic separation module 145 to clean the reaction cup for the first time, and the first incubation disk is detected to obtain a result of the first incubation disk is detected.

Immunoassay devices can be used in a variety of ways, as an example, the immunoassay device is a chemiluminescent immunoassay device (or chemiluminescent device) that combines a chemiluminescent technique with an immune response. The types of samples detected by the immunoassay device include serum, plasma, urine, cerebrospinal fluid or other body fluids.

Chemiluminescent immunoassay devices comprise two components, namely an immunoreaction system and a chemiluminescent assay system. The chemiluminescence analysis system is characterized in that a chemiluminescent substance is catalyzed by a catalyst and oxidized by an oxidant to form an excited intermediate, when the excited intermediate returns to a stable ground state, photons (hM) are emitted simultaneously, and the light quantum yield is measured by a luminescent signal measuring instrument. The immune reaction system is to directly label a luminescent substance (an excited state intermediate generated under the excitation of a reactant) on an antigen (chemiluminescent immunoassay) or an antibody (immunochromatographic immunoassay).

Chemiluminescent immunoassay methods can be classified into three main categories, i.e., direct chemiluminescent immunoassay, enzymatic chemiluminescent immunoassay, and electrochemiluminescent immunoassay, depending on the label. The detection is carried out by adopting a direct chemiluminescence method, in particular to a direct chemiluminescence method based on acridine ester, which is used together with matched detection reagents, and is clinically used for carrying out qualitative or quantitative detection on analytes in clinical common samples such as serum, plasma, urine, cerebrospinal fluid and the like from human bodies, including hormone, tumor-related antigens, infectious diseases, allergen-related projects and the like.

The structure and the detection method of the biochemical analyzer are not particularly limited in the embodiment of the present application, and the biochemical analyzer may further include other structures besides the second sampling needle and the second cleaning module 129.

As an example, as shown in fig. 3, the biochemical analyzer 120 may further include a second incubation plate 122, a cuvette 124, a kit 125, a reagent cartridge 126, a mixing module 127, a third reagent dispensing arm 128, a second washing module 129, and a second control module for controlling operations of these mechanisms, in addition to the second sampling needle 121 and the second detection module 123. The second incubation plate 122 is used to provide a constant temperature environment for the reaction detection of the sample and reagents to occur at approximately 37 ℃ of human temperature.

The working process of the biochemical analyzer comprises the steps that a second sampling needle 121 absorbs samples in a sample tube 134 on a sample bearing unit 133 and discharges the samples into a cuvette 124 on a second incubation tray 122, the second incubation tray 122 rotates the cuvette 124 to a reagent adding position, a reagent needle on a third reagent dispensing arm 128 absorbs reagents from a reagent box 125 in a reagent bin 126 (the reagent bin 126 and the second incubation tray 122 on the outer side can rotate independently), the second incubation tray 122 rotates the cuvette 124 to a mixing position, a mixing module 127 mixes the samples in the cuvette 124 and the reagents uniformly, the cuvette 124 reaches a detection position after being mixed in the second incubation tray 122 for a period of time, and a second detection module 123 detects liquid in the cuvette 124 to obtain a detection result of 1 detection item.

Biochemical analyzers, also commonly referred to as biochemicals, are devices for measuring certain chemical components in serum, plasma and body fluids, and are detection devices that perform some or all of the steps of sampling, adding reagents, removing interferents, mixing, incubating, colorizing, calculating results, cleaning, etc., in biochemical analysis by means of instruments that mimic manual operations. Sample types detected by biochemical analyzers include serum, plasma, urine, cerebrospinal fluid, and the like.

The biochemical analyzer mainly provides various examination items such as clinical biochemistry, clinical hematology, clinical immunology and the like for the examination medicine, is used for the examination of clinical biochemical indexes such as liver function, kidney function, blood fat, diabetes, infection, rheumatism immunity and the like, further provides important scientific basis for the diagnosis, treatment and prevention of diseases, and is a necessary monitoring device for hospitals.

The embodiment of the application does not specifically limit the electrolyte analyzer or the electrolyte analysis module. The electrolyte analyzer or electrolyte analysis module may also be referred to as an ion selective electrode (IonSelectiveElectrode, ISE) module. The ISE analysis module detects ions in the sample using an ion selective electrode method. Ion items that the ISE module can detect are sodium, potassium and chloride. Samples of ISE modules are typically serum and urine.

In some embodiments, the ISE module can be independently detected when the bio-analyzer is on-line, i.e. the electrolyte analyzer, and at this time, the ISE module can be placed outside the bio-analyzer and be arranged as an electrolyte analyzer closely adjacent to the bio-analyzer, and in this case, the ISE module has its own separate sampling needle and shares a power supply with the bio-analyzer.

In other embodiments, the ISE module may also incorporate a single sampling needle for the biochemical analyzer and the biochemical detection module while the bio-engine is on-line. The sampling needle of the biochemical analyzer sucks the sample from the sample tube and discharges the sample into the cuvette of the incubation tray or/and the reaction cell of the ISE module.

The sample analysis system provided by the embodiment of the present application is described in detail above with reference to fig. 1 to 8, and the sample analysis method provided by the embodiment of the present application is described in detail below with reference to fig. 9. It should be appreciated that the method is applicable to the sample analysis system described above, and thus, reference is made to the above where not described and not repeated here.

As described above, the sample analysis system comprises a first sample analyzer comprising a first sampling needle and a first cleaning module, wherein the first sample analyzer is used for detecting a first type of detection item on a sample sucked by the first sampling needle, the first cleaning module is used for cleaning the first sampling needle, a second sample analyzer comprising a second sampling needle, the second sample analyzer is used for detecting a second type of detection item on the sample sucked by the second sampling needle, the second type of detection item is different from the first type of detection item, the second type of detection item comprises an ion detection item, and a sample transportation device is used for transporting a sample carrying unit with a sample tube to the first sample analyzer or/and the second sample analyzer, and the sample tube is provided with the sample.

As shown in fig. 9, the sample analysis method provided by the embodiment of the application includes steps S910 to S920.

In step S910, item information to be detected of the target sample tube is determined.

In step S920, in response to the item information to be detected of the target sample tube including the first type detection item and the second type detection item, the sample carrying unit with the target sample tube placed thereon is transported to the first sample analyzer and the second sample analyzer by the sample transporting device, and the first sampling needle is sequentially cleaned with the cleaning solution and the deionized water by the first cleaning module before the first sampling needle sucks the sample in the target sample tube.

Optionally, the step S920 may specifically include, in response to the item information to be detected of the target sample tube including the first type detection item and the ion detection item, transporting the sample carrying unit with the target sample tube placed therein to the first sample analyzer and the second sample analyzer by using the sample transporting device, and cleaning the first sampling needle with the cleaning liquid, the deionized water, and the gas in sequence by using the first cleaning module before the first sampling needle sucks the sample in the target sample tube.

Alternatively, step S920 may specifically be to, in response to the item information to be detected of the target sample tube including the first type of detection item and the ion detection item including one or more of potassium ion, sodium ion, chloride ion, calcium ion, magnesium ion, and phosphorus ion detection item, transport the sample carrying unit with the target sample tube placed therein to the first sample analyzer and the second sample analyzer using the sample transport device, and wash the first sampling needle with the washing liquid and deionized water in sequence before the first sampling needle sucks the sample in the target sample tube.

Optionally, the method may further include, in response to the item information to be detected of the target sample tube including a first type of detection item, the first type of detection item being a plurality of items, washing the first sampling needle with deionized water only with the first washing module between the first sampling needle sucking the sample in the target sample tube multiple times.

Optionally, the method further comprises responding to the item information to be detected of the target sample tube, wherein the item information to be detected of the target sample tube comprises the first type of detection item and the second type of detection item, the first sample analyzer is an immunity analyzer, and the sample carrying unit with the target sample tube is sequentially conveyed to the first sample analyzer and the second sample analyzer by the sample conveying device.

Optionally, the method further comprises controlling the first sampling needle after cleaning to vertically move to pass through the blowing area of the blowing port, and sucking air by using the first sampling needle when the needle tip of the first sampling needle is about to leave the blowing area so as to form an air section in the first sampling needle.

It should be noted that the elements described in the above embodiments may be combined in any suitable manner without contradiction. The various possible combinations of the application are not described in detail in order to avoid unnecessary repetition.

It will be understood that, although the terms "first" or "second," etc. may be used herein to describe various elements, these elements are not provided by these terms, and these terms are merely used to distinguish one element from another element.

The scope of the present application is not limited to the above embodiments, and any person skilled in the art may conceive of changes or substitutions within the technical scope of the present application, which are intended to be covered by the scope of the present application. Therefore, the protection scope of the application is subject to the protection scope of the claims.

Claims

1. A sample analysis system, comprising:

The first sample analyzer comprises a first sampling needle and a first cleaning module, the first sample analyzer is used for detecting a first type of detection items on a sample sucked by the first sampling needle, and the first cleaning module is used for cleaning the first sampling needle;

The second sample analyzer comprises a second sampling needle, and is used for detecting a second type of detection items on the sample sucked by the second sampling needle, wherein the second type of detection items are different from the first type of detection items, and the second type of detection items comprise ion detection items;

Sample transporting means for transporting a sample carrying unit in which a sample tube having a sample therein is placed to a first sample analyzer or/and a second sample analyzer;

When the information of the items to be detected of the target sample tube comprises the first type detection items and the second type detection items, the sample transporting device is used for transporting the sample carrying unit with the target sample tube to the first sample analyzer and the second sample analyzer, wherein before the first sampling needle absorbs the sample in the target sample tube, the first cleaning module is used for cleaning the first sampling needle by adopting cleaning liquid and deionized water in sequence.

2. The system of claim 1, wherein when the item information to be detected of the target sample tube includes the first type of detection item and the ion detection item, the first cleaning module is configured to sequentially clean the first sampling needle with a cleaning solution and deionized water before the first sampling needle suctions the sample in the target sample tube, wherein the ion detection item includes one or more of a potassium ion, a sodium ion, a chloride ion, a calcium ion, a magnesium ion, and a phosphorus ion detection item.

3. The system of claim 1, wherein when the item information to be detected of the target sample tube includes the first type of detection item and the first type of detection item is a plurality of items, the first sampling needle is configured to aspirate the sample in the target sample tube a plurality of times, and the first cleaning module is configured to clean the first sampling needle with deionized water only between the first sampling needle aspirates the sample in the target sample tube a plurality of times.

4. The system of claim 1, wherein the first cleaning module comprises a cleaning solution supply assembly in communication with the first sampling needle to provide the cleaning solution to the first sampling needle, and a cleaning tank having a deionized water injection port thereon.

5. The system of claim 4, wherein the cleaning tank is further provided with an air blowing port for blowing air onto the outer wall of the first sampling needle which is cleaned.

6. The system of claim 5, wherein the first sample analyzer further comprises an air pump, an overflow valve, and a positive pressure actuator, the air pump in communication with the positive pressure actuator through the overflow valve, the air pump for providing positive pressure to the positive pressure actuator, an overflow end of the overflow valve connected to the air outlet of the purge cell, the overflow valve for supplying overflow air to the purge cell.

7. The system of claim 1, wherein the cleaning fluid comprises an ionic component.

8. The system of claim 7, wherein the cleaning solution comprises one or more of potassium ions, sodium ions, chloride ions, phosphate ions, hydrogen phosphate ions, and dihydrogen phosphate ions.

9. The system of claim 1, wherein the first sample analyzer is an immunoassay analyzer or a hemagglutination analyzer and the second sample analyzer is a biochemical analyzer or an electrolyte analyzer or a biochemical analyzer with electrolyte analysis module.

10. The system according to claim 9, wherein when the item information to be detected of the target sample tube includes the first type of detection item and the second type of detection item, and the first sample analyzer is an immunoassay analyzer, the sample transport means is for sequentially transporting the sample carrying unit on which the target sample tube is placed to the first sample analyzer and the second sample analyzer.

11. The system of claim 1, wherein the sample in the target sample tube is serum, plasma, or urine.

12. The system of claim 5, wherein the first sample needle that completes the purge is adapted to draw air to form an air segment within the first sample needle by moving vertically to pass through an air blowing region of an air blowing port when a needle tip of the first sample needle is about to leave the air blowing region.

13. A method of sample analysis, the method being applied to a sample analysis system, the sample analysis system comprising:

The method comprises the following steps:

determining project information to be detected of a target sample tube;

And responding to the to-be-detected item information of the target sample tube, wherein the to-be-detected item information comprises the first type detection item and the second type detection item, conveying the sample bearing unit with the target sample tube to the first sample analyzer and the second sample analyzer by using the sample conveying device, and cleaning the first sample tube by using the first cleaning module by using cleaning liquid and deionized water before the first sample tube absorbs the sample in the target sample tube.