CN119186657A - Microfluidic chip fluid control system and method - Google Patents
Microfluidic chip fluid control system and method Download PDFInfo
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- CN119186657A CN119186657A CN202310753213.0A CN202310753213A CN119186657A CN 119186657 A CN119186657 A CN 119186657A CN 202310753213 A CN202310753213 A CN 202310753213A CN 119186657 A CN119186657 A CN 119186657A
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
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Abstract
本申请涉及一种微流控芯片流体控制系统和方法。该系统包括：芯片本体和底座，芯片本体包括叠设的接口层、流道层、柔性层和气路层，底座设于气路层的下方；以及，气路层上设有若干气控接口，且底座内设有与若干气控接口连通的若干气路通道，气路通道内可进行充气或抽气，使对应的气控接口内形成正压或负压，改变柔性层与流道层间的腔体体积，定量定向驱动反应液在流道层的功能腔室间移动。本申请提供的方案，能够通过气压控制流体在微流控芯片中定量定向运动，工艺简单、控制精准，以及可操作性强。
The present application relates to a microfluidic chip fluid control system and method. The system includes: a chip body and a base, the chip body includes a stacked interface layer, a flow channel layer, a flexible layer and an air path layer, and the base is arranged below the air path layer; and, the air path layer is provided with a plurality of air control interfaces, and the base is provided with a plurality of air path channels connected to the plurality of air control interfaces, and the air path channels can be inflated or evacuated to form positive or negative pressure in the corresponding air control interface, thereby changing the cavity volume between the flexible layer and the flow channel layer, and quantitatively and directionally driving the reaction liquid to move between the functional chambers of the flow channel layer. The solution provided in the present application can control the quantitative and directional movement of the fluid in the microfluidic chip by air pressure, and has a simple process, precise control, and strong operability.
Description
Technical Field
The application relates to the technical field of microfluidic chips, in particular to a microfluidic chip fluid control system and a microfluidic chip fluid control method.
Background
The microfluidic chip can integrate various biological or chemical programs on one chip, can accurately control micro volume reaction, has small volume and convenient operation, and is a detection platform which is very concerned at present. The movement mode of fluid in the microfluidic chip is an important concern for the design and production of the microfluidic chip.
In the related art, centrifugal microfluidic chips that use centrifugal force to control fluid movement are more. However, this method has high requirements on the manufacturing process of the microfluidic chip, resulting in problems of high manufacturing difficulty and high cost.
Disclosure of Invention
In order to solve or partially solve the problems in the related art, the application provides a microfluidic chip fluid control system and a microfluidic chip fluid control method, which can quantitatively and directionally move in a microfluidic chip by controlling fluid through air pressure, and have the advantages of simple process, accurate control and strong operability.
The application provides a microfluidic chip fluid control system, which comprises a chip body and a base, wherein the chip body comprises an interface layer, a runner layer, a flexible layer and an air path layer which are overlapped, the base is arranged below the air path layer, and
The device comprises a base, a plurality of air control interfaces, a plurality of functional chambers and a plurality of functional chambers, wherein the air control interfaces are arranged on the air circuit layer, the air control interfaces are communicated with the air control channels, and the air channels can be inflated or pumped, so that positive pressure or negative pressure is formed in the corresponding air control interfaces, the volume of the chambers between the flexible layer and the runner layers is changed, and the reaction liquid is quantitatively and directionally driven to move between the functional chambers of the runner layers.
As an optional embodiment, a plurality of air chambers are concavely arranged in the air path layer at one side far away from the base, the air chambers are provided with the air control interfaces towards the base, and when negative pressure is formed in the air control interfaces, the flexible layer bulges towards the air chambers to be separated from the runner layer;
Preferably, the plurality of air chambers together form a plurality of air chamber groups, each air chamber group comprises a main air chamber and a plurality of auxiliary air chambers, the space of the main air chamber is larger than that of the auxiliary air chambers, and the reaction liquid passing through the space of the main air chamber is quantified.
As an alternative embodiment, the lengths of several gas paths are the same.
As an optional embodiment, a disturbance chamber matched with the mixing chamber of the functional chamber is concavely arranged on one side, away from the base, of the air circuit layer, the disturbance chamber is provided with the pneumatic control interface towards the base, and positive and negative pressures can be alternately formed in the disturbance chamber so as to enable the flexible layer between the disturbance chamber and the mixing chamber to vibrate reciprocally.
As an alternative embodiment, the area of the base corresponding to part of the functional chambers is provided with blind holes or through holes;
Preferably, the luminous chamber in the functional chamber is opposite to the blind hole or the through hole, more preferably, the luminous chamber is opposite to the through hole, the through hole is a dense light channel for light signals to pass through, and/or an optical detection component is arranged in the through hole;
Preferably, the mixing chamber in the functional chamber is opposite to the blind hole, and a heating device is arranged in the blind hole.
As an optional embodiment, the base is provided with a containing cavity, and the chip body is embedded in the containing cavity;
preferably, a sealing element is arranged in the accommodating cavity and used for ensuring the air tightness of an air passage between the base and the chip body;
Preferably, the control system further comprises a positioning mechanism, the positioning mechanism comprises a plurality of positioning rods and positioning holes which are respectively arranged on the accommodating cavity and the chip body and are in one-to-one correspondence, and more preferably, the sliding contact surfaces of the positioning rods and the positioning holes are provided with the positioning mechanism.
As an alternative embodiment, the chip body further comprises a gas-liquid separation layer, and the gas-liquid separation layer is disposed between the flow channel layer and the flexible layer.
As an alternative embodiment, the base is made of a heat-conducting material, and preferably the heat-conducting material comprises one of heat-conducting silicone grease, heat-conducting silica gel, heat-conducting graphite, heat-conducting plastic and heat-conducting metal.
As an alternative embodiment, the base includes a first portion and a second portion, the second portion is disposed on a side of the first portion away from the chip body, and a width of the second portion is smaller than a width of the first portion.
The second aspect of the present application provides a microfluidic chip fluid control method, comprising:
s1, placing a chip body in a base;
S2, controlling air pressure in the air path channel through an external air pump, and quantitatively and directionally driving the reaction liquid to move between functional chambers of the flow path layer;
preferably, the step S2 includes:
S21, opening a first auxiliary air chamber, closing a second auxiliary air chamber, opening a main air chamber, and enabling reaction liquid to flow in from a pipeline on the side of the first auxiliary air chamber and fill the main air chamber;
S22, closing the first auxiliary air chamber, opening the second auxiliary air chamber, closing the main air chamber, and discharging the liquid in the main air chamber from the side of the second auxiliary air chamber;
The steps S21 and S22 are repeated.
The technical scheme provided by the application can comprise the following beneficial effects:
According to the embodiment of the application, the air channel is arranged in the base, so that the space of the chip body is not occupied, and the advantages of the micro-fluidic chip are fully exerted. The inlet of the air channel is communicated with the air control interface, an air pump can be externally connected to the outlet of the air channel, and the air pressure in the air channel is controlled by the externally connected air pump. When the external air pump is inflated into the air channel, positive pressure is formed in the air control interface corresponding to the air channel, the volume of the cavity between the flexible layer and the runner layer is reduced, and the reaction liquid in the reaction liquid runner between the flexible layer and the runner layer is extruded, so that the reaction liquid is driven to move between the functional cavities of the runner layer. Therefore, the embodiment of the application realizes the quantitative directional movement of the air pressure control fluid in the chip through the matching of the base and the chip body, and has simple process, accurate control and strong operability.
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the application as claimed.
Drawings
The foregoing and other objects, features and advantages of the application will be apparent from the following more particular descriptions of exemplary embodiments of the application as illustrated in the accompanying drawings wherein like reference numbers generally represent like parts throughout the exemplary embodiments of the application.
Fig. 1 is a schematic structural diagram of a microfluidic chip fluid control system according to an embodiment of the present application;
FIG. 2 is a front view of FIG. 1;
FIG. 3 is a top view of FIG. 1;
FIG. 4 is a left side view of FIG. 3;
FIG. 5 is a cross-sectional view of FIG. 1 at the feed chamber;
Fig. 6 is an exploded view of a microfluidic chip fluid control system according to an embodiment of the present application;
FIG. 7 is a bottom view of the runner layer shown in FIG. 6;
FIG. 8 is a top view of the pneumatic layer shown in FIG. 6;
FIG. 9 is a top view of the flexible layer shown in FIG. 6;
FIG. 10 is a top view of the gas-liquid separator layer shown in FIG. 6;
FIG. 11 is a side view of the base shown in FIG. 6;
FIG. 12 is a bottom view of the base shown in FIG. 6;
fig. 13 is a front view of fig. 12;
FIG. 14 is a schematic diagram showing a state in which both the main valve and the second sub-valve are closed according to the embodiment of the present application;
FIG. 15 is a schematic view showing a state in which a main valve is opened and a second sub-valve is closed according to an embodiment of the present application;
fig. 16 is a schematic view showing a state in which the main valve is closed and the second sub-valve is opened according to the embodiment of the present application.
In the figure:
1. The chip comprises a chip body, 10 parts of interface layers, 11 parts of runner layers, 110 parts of mixing chambers, 111 parts of luminous chambers, 12 parts of flexible layers, 13 parts of gas path layers, 130 parts of pneumatic control interfaces, 131 parts of gas chambers, 131a parts of main gas chambers, 131b parts of auxiliary gas chambers, 131b', first auxiliary gas chambers, 131b ", second auxiliary gas chambers, 132 parts of disturbance chambers, 14 parts of gas-liquid isolation layers, 15 parts of sealing elements;
2. 20 parts of a base, 20 parts of an air passage, 21 parts of a blind hole, 22 parts of a through hole, 23 parts of a containing cavity, 24 parts of a first part, 25 parts of a second part;
3. A set of air cells;
4. A positioning mechanism, 40, a positioning rod, 41, and a positioning hole.
Detailed Description
Embodiments of the present application will be described in more detail below with reference to the accompanying drawings. While embodiments of the present application are illustrated in the drawings, it should be understood that the present application may be embodied in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the application to those skilled in the art.
The terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the application. As used in this specification and the appended claims, the singular forms "a," "an," and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It should also be understood that the term "and/or" as used herein refers to and encompasses any or all possible combinations of one or more of the associated listed items.
It should be understood that although the terms "first," "second," "third," etc. may be used herein to describe various information, these information should not be limited by these terms. These terms are only used to distinguish one type of information from another. For example, first information may also be referred to as second information, and similarly, second information may also be referred to as first information, without departing from the scope of the application. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present application, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.
In the related art, centrifugal microfluidic chips that use centrifugal force to control fluid movement are more. However, this method has high requirements on the manufacturing process of the microfluidic chip, resulting in problems of high manufacturing difficulty and high cost.
Aiming at the problems, the embodiment of the application provides a microfluidic chip fluid control system which can quantitatively and directionally move in a microfluidic chip by controlling fluid through air pressure, and has simple process, accurate control and strong operability. .
The following describes the technical scheme of the embodiment of the present application in detail with reference to the accompanying drawings.
Fig. 1 is a schematic structural view of a microfluidic chip fluid control system according to an embodiment of the present application, fig. 2 is a front view of fig. 1, fig. 3 is a top view of fig. 1, fig. 4 is a left side view of fig. 3, fig. 5 is a sectional view at a feed chamber of fig. 1, fig. 6 is an exploded view of the microfluidic chip fluid control system according to an embodiment of the present application, fig. 7 is a bottom view of a flow channel layer shown in fig. 6, fig. 8 is a top view of a gas channel layer shown in fig. 6, and fig. 9 is a top view of a flexible layer shown in fig. 6.
Referring to fig. 1 to 9, the microfluidic chip fluid control system according to the embodiment of the present application includes a chip body 1 and a base 2, where the chip body 1 includes an interface layer 10, a runner layer 11, a flexible layer 12 and an air path layer 13 that are stacked, and the layers may be combined into a whole by a low-temperature bonding manner, or may be formed into a whole by a double-sided adhesive manner. The base 2 is arranged below the air passage layer 13. The interface layer 10 is provided with a feeding interface for injecting the reaction liquid. Functional chambers are arranged in the runner layer 11, and comprise a feeding chamber, a mixing chamber 110 and a light-emitting chamber 111, wherein the functional chambers are communicated through a liquid runner, and the feeding chamber is communicated with a feeding interface. Wherein, and be equipped with a plurality of air control interfaces 130 on the gas circuit layer 13, and be equipped with a plurality of gas circuit passageway 20 with a plurality of air control interfaces 130 intercommunication in the base 2, can aerify or bleed in the gas circuit passageway 20, make the corresponding air control interface 130 in form malleation or negative pressure, change the cavity volume between flexible layer 12 and runner layer 11, quantitative directional drive reaction liquid is moved between the functional chamber of runner layer 11.
According to the embodiment of the application, the air channel 20 is arranged in the base 2, so that the space of the chip body 1 is not occupied, and the advantages of the microfluidic chip are fully exerted. The inlet of the air channel 20 is communicated with the air control interface 130, and an air pump (not shown in the figure) can be externally connected to the outlet of the air channel 20, and the air pressure in the air channel 20 is controlled by the externally connected air pump. When the external air pump pumps air into the air channel 20, negative pressure is formed in the air control interface 130 corresponding to the air channel 20, the volume of the cavity between the flexible layer 12 and the runner layer 11 is increased, the reaction liquid runner between the flexible layer 12 and the runner layer 11 is filled with the reaction liquid, when the external air pump pumps air into the air channel 20, positive pressure is formed in the air control interface 130 corresponding to the air channel 20, the volume of the cavity between the flexible layer 12 and the runner layer 11 is reduced, and the reaction liquid in the reaction liquid runner between the flexible layer 12 and the runner layer 11 is extruded, so that the reaction liquid is driven to move between the functional cavities of the runner layer 11. Therefore, the embodiment of the application realizes the quantitative directional movement of the air pressure control fluid in the chip through the matching of the base 2 and the chip body 1, and has simple process, accurate control and strong operability.
When the microfluidic chip fluid control system of the embodiment of the application is utilized for detection, firstly, after a sample to be detected, a reagent R1, a reagent R2 and photosensitive liquid enter a feeding chamber through a feeding interface, the air pressure in an air path channel 20 is controlled through an external air pump, the sample to be detected, the reagent R1, the reagent R2 and the photosensitive liquid are quantitatively and directionally driven to flow into a mixing chamber 110 from the respective feeding chambers through liquid flow channels, after a period of reaction in the mixing chamber 110, the reaction liquid in the mixing chamber 110 is driven to flow into a light-emitting chamber 111 through the liquid flow channels through air flow, and finally, the optical signals generated in the light-emitting chamber are detected.
As an alternative embodiment, referring to fig. 6 and 8, a plurality of air chambers 131 are concavely arranged in the air channel layer 13 at the side far away from the base 2, the air chambers 131 are provided with air control interfaces 130 towards the base 2, and when negative pressure is formed in the air control interfaces 130, the flexible layer 12 bulges towards the air chambers 131 to be separated from the runner layer 11.
In the embodiment of the application, the air chamber 131 is concavely arranged at one side of the air path layer 13 far away from the base 2, and the air chamber 131 is provided with the air control interface 130 towards the base 2, so that when negative pressure is formed in the air control interface 130, the air chamber 131 can provide the accommodating space for the flexible layer 12 to bulge to be separated from the runner layer 11, and the air chamber 131 is also a temporary storage space for the reaction liquid in the moving process.
As an alternative embodiment, referring to fig. 8, a plurality of gas cells 131 collectively form a plurality of gas cell groups 3, each gas cell group 3 including one main gas cell 131a and a plurality of sub-gas cells 131b, the space of the main gas cell 131a being larger than the space of the sub-gas cell 131b, and the passing reaction liquid being quantified by the space of the main gas cell 131 a.
When negative pressure is formed in the air passage 20 in the base 2, the flexible layer 12 bulges into the auxiliary air chamber 131b and the main air chamber 131a until the reaction liquid is separated from the runner layer 11, the auxiliary air chamber 131b and the main air chamber 131a are opened, the reaction liquid flows into the auxiliary air chamber 131b and fills the main air chamber 131a, the main air chamber 131a is pumped with the reaction liquid, when positive pressure is formed in the air passage 20 in the base 2, the flexible layer 12 is extruded upwards to be in contact with the runner layer 11, the auxiliary air chamber 131b and the main air chamber 131a are closed, and the reaction liquid is pumped out of the main air chamber 131a, so that the movement of the reaction liquid in the runner layer 11 is quantitatively and directionally driven.
Furthermore, in the embodiment of the present application, the space of the main air chamber 131a is larger than the space of the auxiliary air chamber 131b, so that the liquid passing through the main air chamber 131a can be quantified. When the main air chamber 131a is opened, the size of the space formed is designed to be the amount of liquid pumped once (i.e., the flexible layer 12 swells once), and the amount of liquid passing through is quantified by controlling the number of times of pumping (i.e., the number of times the flexible layer 12 swells).
In addition, at least one auxiliary air chamber 131b is disposed at the liquid inlet end and the liquid outlet end of one main air chamber 131 a. The air cell group 3 of the embodiment of the present application is disposed between two functional chambers in the direction of the liquid flow path, for example, between the feeding chamber and the mixing chamber 110, and between the mixing chamber 110 and the light emitting chamber 111. If there are a plurality of feeding chambers directly communicating with the mixing chamber 110, only one air chamber group 3 is arranged between the plurality of feeding chambers and the mixing chamber, the mixing chamber 110 and the plurality of feeding chambers share one main air chamber 131a, and the number of the plurality of auxiliary air chambers 131b is equal to the sum of the numbers of the mixing chamber 110 and the plurality of feeding chambers.
The air chamber group 3 designed by the embodiment of the application belongs to a microstructure, so that the occupied space is greatly saved, and the function of controlling the liquid flow in a micron-sized flow channel is realized. The whole microfluidic chip has the advantages of small volume, short reaction time and small dosage of reagents and samples.
As an alternative embodiment, see fig. 5, several gas path channels 20 are of the same length.
The lengths of the air channel 20 are the same, so that the air pressure of the same parameter air supplied to the chip body 1 is equal, and the stable movement of the reaction liquid is realized. The number of the air passage channels 20 is equal to that of the air control interfaces 130, and each air passage channel 20 corresponds to one air control interface 130.
As an alternative embodiment, referring to fig. 8, a perturbation chamber 132 matched with the mixing chamber 110 of the functional chamber is concavely arranged on one side of the air path layer 13 away from the base 2, the perturbation chamber 132 is provided with an air control interface 130 towards the base 2, and positive and negative pressures can be alternately formed in the perturbation chamber 132 so as to enable the flexible layer 12 positioned between the perturbation chamber 132 and the mixing chamber 110 to vibrate reciprocally.
Because the flow of the liquid in the micro-flow channel is generally laminar, the liquid is evenly mixed in a nearly diffusion way, and the efficiency is extremely low. Therefore, in order to accelerate the mixing rate of the liquid in the functional chamber, a disturbance chamber 132 with the same area as the mixing chamber 110 is arranged in the air channel layer 13 corresponding to the lower part of the mixing chamber 110, the disturbance chamber 132 is provided with an air control interface 130 towards the base 2, and the air channel 20 is communicated through the air control interface 130. The air is blown and exhausted into the disturbance chamber 132 through the air channel 20 by an external air pump, so that the flexible layer 12 between the disturbance chamber 132 and the mixing chamber 110 vibrates up and down, and the vibration wave is transmitted to the liquid in the mixing chamber 110 through the flexible layer 12, so that the liquid in the mixing chamber 110 reaches a chaotic convection state, and the process of immune reaction is accelerated. Therefore, the chaos convection device is introduced into the microfluidic chip, the defect that the flow of the liquid in the micron-sized flow channel is mostly laminar is overcome, the mixing of the sample and the reagent is accelerated, the reaction efficiency is improved, and the detection sensitivity is improved.
As an alternative embodiment, see fig. 12, the area of the base 2 corresponding to part of the functional chambers is provided with blind holes 21 or through holes 22.
Embodiments of the present application may provide a heating device within blind bore 21 or an optical detection component within through bore 22.
As a preferred embodiment, the light emitting chamber 111 is opposite to the blind hole 21 or the through hole 22.
When the light emitting chamber 111 is opposite to the through hole 22, the through hole 22 may pass an optical signal. In the embodiment of the application, the area of the base 2 corresponding to the light-emitting chamber 111 is not required to be provided with a hole structure, the light-emitting device is arranged at the light-emitting chamber 111 to excite the light signal in the light-emitting chamber 111, the fluorescence generated after the excitation of the laser passes through the chip and is received by the receiving device above the chip, and the concentration of the liquid to be measured is determined by detecting the intensity of the light signal.
As a preferred embodiment, the light emitting chamber 111 is opposite to the through hole 22, and the through hole 22 is a dense light channel through which the light signal passes, and/or an optical detection part is provided in the through hole 22.
When the optical detection member is not provided in the through hole 22, the optical detection member may be provided at a position corresponding to the through hole 22 outside the chassis 2, and the optical signal emitted from the through hole 22 may be detected. Or an optical detection member is provided in the through hole 22, and optical signal detection is directly performed in the through hole 22.
As a preferred embodiment, the mixing chamber 110 is opposite to the blind hole 21, and a heating device is provided in the blind hole 21.
By providing a heating device in the blind hole 21 corresponding to the mixing chamber 110, the mixed liquid in the mixing chamber 110 is heated, the temperature required for the reaction is provided, and the reaction efficiency is improved.
As an alternative embodiment, referring to fig. 6 and 13, the base 2 is provided with a receiving cavity 23, and the chip body 1 is embedded in the receiving cavity 23.
The embodiment of the application realizes the stable connection of the chip body 1 and the base 2 by arranging the accommodating cavity 23, and the one-to-one corresponding connection of the air channel 20 and the air control interface 130, and prevents the movement of the chip body 1 on the base 2.
As a preferred embodiment, referring to fig. 6, a sealing member 15 is provided in the accommodating chamber 23, and the sealing member 15 is used to ensure airtightness of the air passage between the base 2 and the chip body 1.
The sealing element 15 can be a sealing gasket or a sealing ring, so that the air flow does not escape when flowing in the air path layer 13 and the air path channel 20, and the pressure of the air pressure is ensured.
As an alternative embodiment, the chip body 1 further comprises a gas-liquid separation layer 14, the gas-liquid separation layer 14 being arranged between the runner layer 11 and the flexible layer 12.
Because the air flow in the air control interface 130 in the air path layer 13 has a certain pressure, a certain extrusion action is generated on the flexible layer 12, and the reaction liquid in the runner layer 11 is driven to move by the extrusion of the flexible layer 12, the lower part of the flexible layer 12 is subjected to the air flow action, and the upper part is subjected to the fluid action, so that the separation of the reaction liquid above the flexible layer 12 from the air flow below is realized by arranging the air-liquid separation layer 14. And the thickness of the flexible layer 12 is not large due to the influence of the volume of the microfluidic chip, and the flexible layer 12 can be prevented from being damaged by isolating the flexible layer 12.
As an alternative embodiment, referring to fig. 6 to 13, the control system further includes a positioning mechanism 4, and the positioning mechanism 4 includes a plurality of positioning rods 40 and positioning holes 41 that are disposed on the accommodating cavity 23 and the chip body 1 in a one-to-one correspondence.
The positioning rod 40 and the positioning hole 41 of the embodiment of the present application cooperate to restrict the movement of the chip body 1 in the horizontal direction, and do not restrict the movement of the chip body 1 in the vertical direction (movement toward and away from the base 2). When in use, the positioning hole 41 of the whole chip body 1 is aligned with the positioning rod 40 on the accommodating cavity 23 and is placed into the base 2, and the detection process is started.
Preferably, the sliding contact surface of the positioning rod 40 and the positioning hole 41 is provided with a positioning mechanism.
The vertical movement of the chip body 1 is limited by the positioning mechanism 4, which comprises a plurality of resisting pieces and a plurality of locking pieces, wherein the resisting pieces and the locking pieces are respectively arranged on the sliding contact surfaces of the positioning rod 40 and the positioning hole 41. And the blocking member may be an annular groove and the locking member may be an annular protrusion.
As an alternative embodiment, the base 2 is made of a heat-conducting material, preferably, the heat-conducting material includes one of heat-conducting silicone grease, heat-conducting silica gel, heat-conducting graphite, heat-conducting plastic and heat-conducting metal.
In the detection process, the reaction chamber in the chip body 1 needs to be heated to reach the temperature required by the reaction, so that the immune reaction process is improved. The too high temperature of the base 2 may affect the quality of the base 2, and even deform the gas path 20 in the base 2, which ultimately affects the movement of the reaction solution. Therefore, the base 2 is made of the heat conducting material, so that good heat conducting performance of the base 2 is realized, heat generated by reaction can be timely conducted out, and the phenomenon that the quality of the chip body 1 is affected due to the fact that the temperature is concentrated in the chip body 1 and cannot be dispersed can be avoided.
As an alternative embodiment, referring to fig. 2, 4,5 and 11, the base 2 includes a first portion 24 and a second portion 25, the second portion 25 is disposed on a side of the first portion 24 away from the chip body 1, and a width of the second portion 25 is smaller than a width of the first portion 24.
The embodiment of the application enables the air passage 20 in the base 2 to be arranged into a plurality of layers, such as two layers in fig. 5, by arranging the base 2 into a layered structure which is longitudinally distributed. The air passage 20 may be disposed in both the first portion 24 and the second portion 25, so that the number of air passages 20 is greater, the distribution is not interfered with each other, and more air control interfaces 130 can be controlled.
Corresponding to the embodiment of the application function implementation method, the application also provides a microfluidic chip fluid control method and a corresponding embodiment.
The microfluidic chip fluid control method of the embodiment of the application comprises the following steps:
s1, placing the chip body 1 in the base 2.
The chip body 1 comprises an interface layer 10, a runner layer 11, a flexible layer 12 and an air path layer 13 which are stacked. The air passage layer 13 is provided with a plurality of air control interfaces 130, and the base 2 is internally provided with a plurality of air passage channels 20 communicated with the plurality of air control interfaces 130. The base 2 is provided with a containing cavity 23, and the chip body 1 is embedded in the containing cavity 23. The chip body 1 is firmly connected with the base 2 through the accommodating cavity 23, the air passage 20 and the air control interface 130 are connected in a one-to-one correspondence manner, and the movement of the chip body 1 on the base 2 is prevented.
S2, controlling the air pressure in the air channel 20 through an external air pump, and quantitatively and directionally driving the reaction liquid to move between the functional chambers of the runner layer 11.
In the embodiment of the application, the air channel 20 can be inflated or exhausted by an external air pump, so that positive pressure or negative pressure is formed in the corresponding air control interface 130, the cavity volume between the flexible layer 12 and the runner layer 11 is changed, and the reaction liquid is quantitatively and directionally driven to move between the functional cavities of the runner layer 11.
According to the embodiment of the application, the air channel 20 is arranged in the base 2, so that the space of the chip body 1 is not occupied, and the advantages of the microfluidic chip are fully exerted. The inlet of the air channel 20 is communicated with the air control interface 130, and an air pump (not shown in the figure) can be externally connected to the outlet of the air channel 20, and the air pressure in the air channel 20 is controlled by the externally connected air pump. When the external air pump pumps air into the air channel 20, negative pressure is formed in the air control interface 130 corresponding to the air channel 20, the volume of the cavity between the flexible layer 12 and the runner layer 11 is increased, the reaction liquid runner between the flexible layer 12 and the runner layer 11 is filled with the reaction liquid, when the external air pump pumps air into the air channel 20, positive pressure is formed in the air control interface 130 corresponding to the air channel 20, the volume of the cavity between the flexible layer 12 and the runner layer 11 is reduced, and the reaction liquid in the reaction liquid runner between the flexible layer 12 and the runner layer 11 is extruded, so that the reaction liquid is driven to move between the functional cavities of the runner layer 11. Therefore, the embodiment of the application realizes the quantitative directional movement of the air pressure control fluid in the chip through the matching of the base 2 and the chip body 1, and has simple process, accurate control and strong operability.
When the microfluidic chip fluid control system of the embodiment of the application is utilized for detection, firstly, after a sample to be detected, a reagent R1, a reagent R2 and photosensitive liquid enter a feeding chamber through a feeding interface, the air pressure in an air path channel 20 is controlled through an external air pump, the sample to be detected, the reagent R1, the reagent R2 and the photosensitive liquid are quantitatively and directionally driven to flow into a mixing chamber 110 from the respective feeding chambers through liquid flow channels, after a period of reaction in the mixing chamber 110, the reaction liquid in the mixing chamber 110 is driven to flow into a light-emitting chamber 111 through the liquid flow channels through air flow, and finally, the optical signals generated in the light-emitting chamber are detected.
Fig. 14 is a schematic view showing a state where both the main valve and the second sub-valve are closed, fig. 15 is a schematic view showing a state where the main valve is opened and the second sub-valve is closed, and fig. 16 is a schematic view showing a state where the main valve is closed and the second sub-valve is opened.
As an alternative embodiment, referring to fig. 14 to 16, taking a left side flow inlet as an example, step S2 includes:
S21, the first auxiliary gas chamber 131b ' is opened, the second auxiliary gas chamber 131b ' is closed, the main gas chamber 131a is opened, and the reaction solution flows into the main gas chamber 131a from the side of the first auxiliary gas chamber 131b '.
The air passage 20 corresponding to the first auxiliary air chamber 131b 'and the main air chamber 131a is pumped by the external air pump, and the air passage 20 corresponding to the second auxiliary air chamber 131b″ is inflated by the external air pump, so that negative pressure is formed in the first auxiliary air chamber 131b' and the main air chamber 131a, positive pressure is formed in the second auxiliary air chamber 131b ', the flexible layer 12 positioned at the first auxiliary air chamber 131b' and the main air chamber 131a bulges into the first auxiliary air chamber 131b 'and the main air chamber 131a until the flexible layer 12 is separated from the gas-liquid separation layer 14, and the flexible layer 12 positioned at the second auxiliary air chamber 131b″ is contacted with the gas-liquid separation layer 14, and the reaction liquid flows into the main air chamber 131a from the side pipeline of the first auxiliary air chamber 131b' and fills the main air chamber 131a.
S22, closing the first auxiliary air chamber 131b ', opening the second auxiliary air chamber 131b ', closing the main air chamber 131a, and discharging the liquid in the main air chamber 131a from the side of the second auxiliary air chamber 131b '.
Then the air channel 20 corresponding to the first auxiliary air chamber 131b 'and the main air chamber 131a is inflated by the external air pump, and the air channel 20 corresponding to the second auxiliary air chamber 131b″ is deflated by the external air pump, so that positive pressure is formed in the first auxiliary air chamber 131b' and the main air chamber 131a, negative pressure is formed in the second auxiliary air chamber 131b ', the flexible layer 12 positioned at the first auxiliary air chamber 131b' and the main air chamber 131a is in contact with the gas-liquid separation layer 14, the flexible layer 12 positioned at the second auxiliary air chamber 131b″ bulges into the second auxiliary air chamber 131b″ to be separated from the gas-liquid separation layer 14, and the reaction liquid flows into the second auxiliary air chamber 131b″ from the main air chamber 131a side pipeline and is discharged from the second auxiliary air chamber 131b″ side.
And (5) repeating the steps S21 and S22 to realize the directional quantitative transfer of the liquid.
Therefore, the embodiment of the application realizes the quantitative directional movement of the air pressure control fluid in the chip through the matching of the base 2 and the chip body 1, and has simple process, accurate control and strong operability.
The specific manner in which the respective modules perform the operations in the apparatus of the above embodiments has been described in detail in the embodiments related to the method, and will not be described in detail herein.
The foregoing description of embodiments of the application has been presented for purposes of illustration and description, and is not intended to be exhaustive or limited to the embodiments disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the various embodiments described. The terminology used herein was chosen in order to best explain the principles of the embodiments, the practical application, or the improvement of technology in the marketplace, or to enable others of ordinary skill in the art to understand the embodiments disclosed herein.
Claims (10)
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| CN119386945A (en) * | 2024-12-31 | 2025-02-07 | 北京中医药大学 | Microfluidic chip and application method |
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