CN118717626A - A soothing composition containing bamboo leaf extract, cream and preparation method thereof - Google Patents

Abstract

The present invention discloses a soothing composition containing a leaf extract of Bambusa chinensis, wherein the active ingredient is composed of the following components in parts by mass: 1-5 parts of leaf extract of Bambusa chinensis, 1-8 parts of extract of Herba Lycopodii. The present invention also discloses a cream comprising the soothing composition, wherein the raw materials include, by mass, 3-12 parts of the soothing composition, 0.01-0.04 parts of a chelating agent, 5-20 parts of a moisturizing agent, 0.1-1 parts of a pH adjusting agent, 0.2-2 parts of a thickening agent, 5-20 parts of an emollient, 1-5 parts of an emulsifier, and 0.01-0.3 parts of a skin conditioning agent. The soothing composition of the present invention is all natural active ingredients, which are less irritating and less dependent than synthetic anti-allergic soothing agents, and meet the cosmetic specifications.

Description

A soothing composition containing bamboo leaf extract, cream and preparation method thereof

Technical Field

The present invention relates to the field of A61K8/9789, and in particular to a soothing composition containing a bamboo leaf extract, a cream and a preparation method thereof.

Background Art

Sensitive skin refers specifically to a highly reactive state of the skin under physiological or pathological conditions, mainly occurring on the face. Clinically, the skin is prone to subjective symptoms such as burning, stinging, itching, and tightness when stimulated by physical, chemical, mental and other factors, with or without objective signs such as erythema, scaling, and capillary dilation. According to the "Chinese Expert Consensus on the Diagnosis and Treatment of Sensitive Skin", current research believes that the occurrence of sensitive skin is a "complex process involving the skin barrier-neurovascular-immune inflammation". Under the interaction of internal and external factors, the skin barrier function is damaged, causing an increase in sensory nerve afferent signals, resulting in increased skin reactivity to external stimuli and triggering skin immune inflammatory reactions.

The sensitive skin care market is growing steadily as consumer awareness increases. At the same time, population diversity and demand for functional segmentation continue to drive future growth. The population with sensitive skin is large, including not only most women, a small number of men, but also babies.

Currently, chemically synthesized antiallergic agents have strong dependence and side effects. Although these compounds have good anti-allergic effects, most of them are banned substances in cosmetics. Therefore, screening natural substances with anti-allergic effects and natural active ingredients allowed by regulations to be used in cosmetics has a good market prospect.

Chinese patent CN111184664B discloses an anti-allergic composition comprising cyperus rotundus and echium, which has an inhibition rate of up to 95% on hyaluronidase, and the two synergistically enhance the effect, which can improve sensitive skin. Chinese patent CN109077970B discloses an anti-allergic natural active ingredient composition comprising cyperus rotundus, hibiscus leaves, rhodiola rosea, okra, and dihydroquercetin, which achieves an anti-allergic effect by inhibiting the release of β-hexosidase and histamine by RBL-2H3 cells. However, the above anti-allergic composition has a single efficacy, and while it is anti-allergic, it is also important to repair the damaged skin barrier.

Summary of the invention

In view of the above problems, the present invention discloses a soothing composition containing Lophatherum gracile Brongn. extract, wherein the active ingredients are composed of the following components in parts by mass: 1-5 parts of Lophatherum gracile Brongn. extract and 1-8 parts of Cardiospermum halicacabum extract.

Preferably, the active ingredient is composed of the following components in parts by mass: 1-4 parts of Bambusa chinensis leaf extract and 2-6 parts of Cyperus rotundus extract.

The second object of the present invention is to provide a cosmetic containing the soothing composition, wherein the cosmetic is a liquid cosmetic, a solid cosmetic, or a semi-solid cosmetic.

The liquid cosmetics include at least one of emulsion, body honey, body lotion, toner, skin care lotion, spray, and body oil; the emulsion refers to an emulsion-like product with a flowing or semi-flowing appearance, such as essence milk, body milk, moisturizing milk, body honey, etc.; the liquid cosmetics also refer to watery or slightly viscous products, such as toner, firming lotion, skin care lotion, etc.; the body oil refers to a product with a transparent oily liquid appearance and a flowing state.

The solid cosmetics include at least one of skin care powder, cleansing mud, face washing mud, cream, moisturizing cream, and hand wax; the solid cosmetics refer to paste-like and cream-like products that are not easy to flow.

The semi-solid cosmetics include at least one of gel and curry; the semi-solid cosmetics refer to products with a transparent or translucent gel or semi-fluid viscous appearance.

Preferably, the soothing composition is added to the cosmetic in an amount of 1-20% by weight; preferably 1-10%.

The emulsion includes emulsifiers, skin conditioners, preservatives, flavors, water, chelating agents, thickeners, etc.; the gel cosmetics also include high molecular polymers; the moisturizing oil also includes mineral oil, animal and plant oils, artificial synthetic oils, etc.; in order to meet other functions of the cosmetics, an appropriate amount of active additives can also be added.

Preferably, the cosmetic is a cream, and the raw materials include, by weight: 3-12 parts of a soothing composition, 0.01-0.04 parts of a chelating agent, 5-20 parts of a moisturizer, 0.1-1 parts of a pH regulator, 0.2-2 parts of a thickener, 5-20 parts of an emollient, 1-5 parts of an emulsifier, and 0.01-0.3 parts of a skin conditioner.

Preferably, the raw materials include, by mass: 10 parts of soothing composition, 0.02-0.03 parts of chelating agent, 10-15 parts of moisturizing agent, 0.15-0.3 parts of pH adjusting agent, 0.5-1 parts of thickening agent, 10-15 parts of emollient, 2-4 parts of emulsifier, and 0.05-0.15 parts of skin conditioning agent.

In one embodiment, the chelating agent is selected from one or more of an inorganic chelating agent and an organic chelating agent.

Preferably, the chelating agent is an organic chelating agent, including but not limited to disodium EDTA, tetrasodium EDTA, and sodium phytate.

Further preferably, the chelating agent is disodium EDTA.

In one embodiment, the humectant is selected from one or more of polyols, amino acids, and polysaccharides.

Preferably, the humectant is a polyol, including but not limited to at least one of glycerin, butylene glycol, 1,2-pentanediol, 1,2-hexanediol, polyethylene glycol-32, dipropylene glycol, sorbitol, 1,3-propylene glycol, and sucrose.

More preferably, the moisturizing agent is a combination of glycerol, butylene glycol, and 1,2-pentanediol in a mass ratio of (3-12):(1-5):(1-3).

In one embodiment, the skin conditioning agent is selected from at least one of Tremella fuciformis Berk extract, panthenol, sodium hyaluronate, allantoin, raffinose, trehalose, dipotassium glycyrrhizate, niacinamide, adenosine, acetyl glucosamine, caprylyl glycol, and glyceryl polyether-26.

Preferably, the skin conditioner is a combination of Tremella fuciformis fruiting body extract and dipotassium glycyrrhizinate in a mass ratio of (0.005-0.25): (0.005-0.05).

Bamboo leaves contain flavonoids, phenolic acids, triterpenes, steroids and other effective ingredients. Bamboo leaf extract has antibacterial, anti-oxidant, anti-inflammatory and vasoconstrictor effects. Studies have found that the phenolic acids in bamboo leaves have a strong anti-inflammatory effect and can be used in cosmetics to soothe the skin. However, the applicant found in a large number of studies that the soothing ability of Bamboo leaf extract alone is limited, while the addition of the extract of the Herba Lycopodii, especially when the mass ratio is (1-5): (1-8), can further improve its soothing ability and also be anti-allergic. In addition, with the help of other adjuvants, the damaged skin barrier can be repaired together. The applicant believes that the possible reason is that the extract of the Psoralea corylifolia is rich in unsaturated fatty acids, flavonoids, terpenes, sterols and other chemical components, which can prevent damage inside and outside cells and effectively relieve inflammation. It works together with the phenolic acid in the extract of the bamboo leaves to improve the soothing ability. The Tremella fuciformis fruiting body is a material-derived hyaluronic acid-like substance. The water-soluble high-molecular heteropolysaccharide extracted from Tremella fuciformis has moisturizing properties that surpass hyaluronic acid and can retain 490 times more water, thereby providing further repair to damaged skin.

In one embodiment, the pH regulator is selected from at least one of arginine, tromethamine, and aminomethylpropanediol.

Preferably, the pH regulator is tromethamine.

In one embodiment, the thickener is selected from at least one of xanthan gum, polyacrylamide, polyacrylate crosspolymer-6, acrylic acid (ester) copolymer/C10-30 alkyl acrylate crosspolymer, sodium acrylate/sodium acryloyldimethyl taurate copolymer, carbomer, ammonium acryloyldimethyl taurate/VP copolymer, hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer, and vinyl polydimethylsiloxane/polymethylsiloxane silsesquioxane crosspolymer.

Preferably, the thickener is a copolymer of hydroxyethyl acrylate/sodium acryloyldimethyl taurate and an acrylic acid (ester)/C10-30 alkyl acrylate cross-linked polymer, with a mass ratio of (0.06-0.7): (0.14-1.3).

In one embodiment, the emollient is selected from one or more of natural organic compounds and chemically synthesized oils.

Including but not limited to caprylic/capric triglyceride, hydrogenated polydecene, caprylic/capric/myristic/stearic triglyceride, octyldodecanol myristate, ethylhexyl palmitate, decyl cocoate, diisostearyl malate, pentaerythrityl tetra(ethylhexanoate), hydrogenated C6-14 olefin polymers, coconut oil, mangifera indica seed butter, jojoba seed oil, sunflower seed oil, meadowfoam seed oil, macadamia ternifolia seed oil, avocado butter, prunus amygdalus dulcis oil, squalane, tocopheryl acetate, cetyl ethylhexanoate, dimethicone.

Preferably, the emollient is a combination of squalane, cetyl ethylhexanoate and polydimethylsiloxane in a mass ratio of 3:(1-12):(1-5).

In one embodiment, the emulsifier is selected from one or more of surfactant emulsifiers, natural emulsifiers, and auxiliary emulsifiers.

Preferably, the emulsifier is a surfactant emulsifier, including but not limited to polysorbate-20, polysorbate-60, glyceryl stearate, glyceryl stearate SE, PEG-100 stearate, polyglyceryl-3 methyl glucose distearate, PEG-60 hydrogenated castor oil, ceteth-20, cetearyl glucoside, sorbitan stearate, sorbitan sesquioleate, cetearyl alcohol, cetearyl olive oil ester, sorbitan olive oil ester, C12-20 alkyl glucoside, and octyldodecanol polyether-25.

Preferably, the emulsifier is a combination of cetearyl alcohol, C12-20 alkyl glucoside and glyceryl stearate in a mass ratio of (0.5-2.5):(0.3-1.2):(0.2-1.3).

In one embodiment, the preservative is selected from at least one of phenoxyethanol, ethylhexylglycerin, and p-hydroxyacetophenone.

Preferably, the preservative is ethylhexylglycerin.

The preparation method of the cream comprises the following steps:

(1) Water, chelating agent, moisturizing agent, skin conditioning agent, and pH adjusting agent are raw materials of phase A; the raw materials of phase A are mixed, heated to 70-80° C., dissolved completely, and kept warm for later use;

(2) Emollients and emulsifiers are raw materials for phase C; the raw materials for phase C are mixed, heated to 70-80° C., dissolved completely, and kept warm for later use;

(3) The preservative and soothing composition are the raw materials of phase D; the raw materials of phase D are mixed, stirred evenly, and set aside;

(4) Add the thickener of phase B into phase A and homogenize for 3-5 minutes at 70-80°C;

(5) Add phase C to the contents obtained in step (4), homogenize and emulsify for 5-8 minutes at 70-80°C;

(6) Cool the contents obtained in step (5) to 40-45° C., add phase D, and homogenize for 3-5 minutes to obtain the cream.

Beneficial Effects

1. The soothing composition of the present invention can not only repair damaged skin barriers, but also relieve chronic skin inflammation (chemical irritation, atopic eczema) and relieve acute skin irritation (insect bites, razor injuries).

2. The soothing compositions of the present invention are all active ingredients of natural origin, which are less irritating and less dependent than synthetic anti-allergic soothing agents, and meet the cosmetic standards; they can be widely used in various cosmetics.

3. The cream formula of the present invention is mild, non-irritating, strong, efficient and safe, and the good ratio between the soothing components achieves the best synergistic effect.

BRIEF DESCRIPTION OF THE DRAWINGS

Figure 1 Results of skin color parameters (a value) of the human skin SLS patch soothing test

Figure 2 Heme detection results of human skin SLS patch soothing test

DETAILED DESCRIPTION

The formulations of Examples 1-6 and Comparative Examples 1-3 are shown in Table 1 below, in parts by mass.

Table 1 Composition of a cream formula containing bamboo leaf extract

The Tremella fruiting body extract was purchased from Shanghai Huiwen Biotechnology Co., Ltd.

The hydroxyethyl acrylate/sodium acryloyldimethyl taurate copolymer was purchased from SEPPIC, France.

The acrylic acid (ester)/C10-30 alkyl acrylate crosspolymer was purchased from LUBRIZOL ADVANCEDMATERIALS, INC. (50025235).

The polydimethylsiloxane was purchased from Dow Corning-China Co., Ltd.

The C12-20 alkyl glucoside was purchased from SEPPIC (50025152) of France.

The extract of Bambusa longituba was purchased from CM TECH CHINA Co., Ltd.

The Herba Lysimachiae extract was purchased from RAHN Group.

The creams described in Examples 1-6 and Comparative Examples 1-3 are specifically prepared by the following method:

(1) Water, chelating agent, moisturizing agent, skin conditioning agent, and pH adjuster are raw materials of phase A; the raw materials of phase A are mixed, heated to 75° C., dissolved completely, and kept warm for later use;

(2) Emollients and emulsifiers are raw materials for phase C; the raw materials for phase C are mixed, heated to 75° C., dissolved completely, and kept warm for later use;

(3) The preservative and soothing composition are the raw materials of phase D; the raw materials of phase D are mixed, stirred evenly, and set aside;

(4) Add the thickener of phase B into phase A and homogenize for 4 minutes at 75°C.

(5) Add phase C to the contents obtained in step (4) and homogenize and emulsify for 5 minutes at 75°C.

(6) Cool the contents obtained in step (5) to 43° C., add phase D, and homogenize for 4 minutes to obtain the cream.

Performance Testing

1. Skin barrier repair test

45 volunteers aged 18-60 years old with damaged facial skin barrier (except pregnant or lactating women) were selected and divided into 9 groups, with 5 people in each group. The creams prepared in Examples 1-6 and Comparative Examples 1-3 were used once in the morning and evening every day. The transepidermal water loss rate of the face was measured with a transepidermal water loss test probe Tewameter (CK, Germany) before use of the product and after continuous use for 4 weeks. The same point was measured 5 times, and the average value of 3 times was recorded after excluding the maximum and minimum values.

Transepidermal Water Loss (TEWL) reflects the barrier function of the stratum corneum and can be used to evaluate the ability of cosmetics to repair the skin barrier. Under the test conditions, the higher the TEWL value obtained, the more transepidermal water loss per unit time and per unit cross-sectional area, and the poorer the skin barrier function. On the contrary, the better the skin barrier function, that is, the more the TEWL value decreases, the stronger the product's ability to repair the skin barrier.

Tewl value change rate = (Tewl value after 4 weeks of use of the product - Tewl value before use) ÷ Tewl value before use × 100%.

Experimental results: The results are shown in Table 2. All the embodiments and comparative examples are effective, but the comparative examples have limited ability to repair the skin barrier.

Table 2 Transepidermal water loss Tewl value change rate

2. Human skin SLS patch soothing test

Sample Information

Creams prepared in Examples 1-6 and Comparative Examples 1-3

Purpose of the test

Sodium lauryl sulfate (SLS) occluded patches on the skin can cause skin damage, which may be accompanied by local erythema and itching. The soothing effect of the test substance can be determined by testing the intervention of the test substance on the skin damage reaction caused by SLS.

Pilot Project

Patch test is a simple and reliable method to determine the allergens of patients with dermatitis and eczema. The suspected allergen is prepared into a certain concentration, placed in a special chamber and applied to the covered part of the human body (usually on the back and the flexor side of the forearm). After a certain period of time, whether the test substance is an allergen (i.e., a sensitizing substance) is determined based on whether there is a positive reaction. SLS is a common positive component of irritation reaction. Placing a certain concentration of SLS in the chamber of the patch tester and acting on the skin can cause skin barrier damage and induce inflammation, which can be manifested as local dermal small blood vessel dilation, increased blood flow, increased skin hemoglobin (Ervthema) and transepidermal water loss (TEWL), and decreased skin water content. Therefore, by placing SLS and the test substance in the chamber of the patch tester at the same time, or applying the test substance after skin damage, and detecting the changes in skin hemoglobin, TEWL, and water content at a specified time and in a specified area, the soothing effect of the test substance can be reflected to a certain extent. This test mainly detects the changes in skin a value and hemoglobin to prove it.

Preparation before the test

(1) Subject screening

The exclusion criteria for subjects are as follows:

a. Those who have used antihistamines in the past week or immunosuppressants in the past month;

b. Those who have used any anti-inflammatory drugs in the tested area within the past two months;

c. Subjects with inflammatory skin diseases that have not been clinically cured;

d. Patients with insulin-dependent diabetes mellitus;

e. Patients who are receiving treatment for asthma or other chronic respiratory diseases;

f. Those who have received anti-cancer chemotherapy in the past 6 months;

g. Patients with immunodeficiency or autoimmune diseases;

h. Women who are breastfeeding or pregnant;

i. Bilateral mastectomy and bilateral axillary lymph node removal;

j. The judges of the test results may be affected by scars, pigmentation, atrophy, port wine stains or other blemishes on the skin to be tested;

k. Participating in other clinical trial researchers;

l. Those with highly sensitive constitution;

m. Non-voluntary participants or those who cannot complete the required content according to the test requirements

(2) Setting the number of subjects

This test outputs more than 5 cases of valid data

(3) Instruments and reagents

Table 3: List of instruments and reagents

Instrument/reagent name brand Model/Article No. Remark MPA6 CK - Self-calibration SLS Sigma 71725-50G Store at room temperature Single channel pipette Brand 200ul Calibrated

Test methods

(1) Experimental design

The test was conducted in a constant temperature and humidity test room with a test period of 24 hours. A total of 3 test areas were selected, divided into a blank control, an SLS treatment group, and an SLS combined with a test substance treatment group (including combined Examples 1, 3, 6 and Comparative Examples 1, 2, 3) (24 hours), and the blank control group was not treated in any way.

The test plan is: select 3 test areas suitable for the patch tester, apply the test substance to a designated area first, and then perform the patch test. After 24 hours, remove the patch tester and detect the changes in each indicator.

Detection indicators: Use MX18 skin probe and CL400 probe to detect hemoglobin and skin color before and after use at a specified time.

(2) Product Usage

Apply the test substance once to the test area, wait for it to be completely absorbed and dry, then stick a spot tester chamber with 2% SLS added. Remove the spot tester after 24 hours.

(3) Test process

The test site selected was the left and right forearm flexion side, 3 marked areas, and the specific test steps were as follows:

a. Wash the entire arm with clean water and equilibrate it in the room (August 26: 21.3℃, 50.7%; August 27: 21.8℃, 50%) for 20 minutes;

b. Use the MX18 probe to detect the initial hemoglobin, repeat the test 3 times for each area, and take the average value as the final test value; use the CL400 probe to detect the initial skin color parameters, repeat the test 3 times for each area, and take the average value as the final test value;

c. Apply the test substance to the designated test area and wait for it to be completely absorbed and dry;

d. Pipette 25uL of 2% SLS solution and drop it onto the filter paper attached to the spot tester and place it in the small chamber;

e. Apply the spot tester to the skin on the flexor side of the subject's forearm with a non-irritating tape, and gently press with the palm of your hand to make it evenly applied for 24 hours;

f. Remove the spot tester, clean the test area, and balance it in the room for 30 minutes. After the indentation disappears, observe the skin reaction;

g. Repeat step b and record each test value as the immediate relief test value;

h. Statistical data and analysis results.

Test results

(1) Calculation formula

a value reduction rate % = (a1-a2)/a1x100%

Hemoglobin reduction rate % = (MX1-MX2)/MX1x100%

Where:

a1—a value of SLS group; a2—a value of SLS+test substance group;

MX1—hemoglobin in the SLS group; MX2—hemoglobin in the SLS+test substance group.

(2) Results Analysis

The test data of hemoglobin and skin color parameters (a value) of 10 subjects are shown in the following table:

a value detection value

Hemoglobin test value

A total of 10 subjects were included in the soothing test, and the skin color parameters (a value) and hemoglobin test results are shown in Figures 1 and 2 below. After SLS patch treatment, a value and hemoglobin increased significantly (P < 0.01, P < 0.001). Compared with the 2% SLS group, the a value reduction rates of Examples 1, 3, 6 and Comparative Examples 1, 2, 3 were 2%, 7%, 13%, 1%, 5%, and 6%, respectively; the hemoglobin reduction rates were 3.2%, 4.9%, 7.8%, 2.6%, 4.6%, and 4.7%, respectively.

Claims (10)

1. The soothing composition containing the lophatherum gracile extract is characterized by comprising the following active ingredients in parts by weight: 1-5 parts of lophatherum gracile extract and 1-8 parts of ringer extract.

2. The soothing composition according to claim 1, wherein the active ingredient consists of the following components in parts by mass: 1-4 parts of lophatherum gracile extract and 2-6 parts of ringer extract.

3. A cosmetic product comprising a soothing composition according to any of claims 1 to 2, characterized in that it is a liquid cosmetic, a solid cosmetic, a semi-solid cosmetic.

4. The cosmetic product according to claim 2, wherein the liquid cosmetic product comprises at least one of an emulsion, a skin lotion, a spray, a skin oil; the solid cosmetic comprises at least one of skin care powder, facial cleanser, cream, skin cream and hand wax; the semi-solid cosmetic comprises at least one of gel and curry.

5. The cosmetic according to claim 4, wherein the cream comprises, in parts by mass: 3-12 parts of a soothing composition, 0.01-0.04 part of a chelating agent, 5-20 parts of a humectant, 0.1-1 part of a pH regulator, 0.2-2 parts of a thickener, 5-20 parts of an emollient, 1-5 parts of an emulsifier and 0.01-0.3 part of a skin conditioner.

6. The cosmetic product according to claim 5, wherein the chelating agent is selected from one or more of an inorganic chelating agent and an organic chelating agent.

7. The cosmetic according to claim 5, wherein the humectant is one or more selected from the group consisting of a polyhydric alcohol, an amino acid, and a polysaccharide.

8. The cosmetic product according to claim 5, wherein the humectant is a polyhydric alcohol comprising at least one of glycerin, butylene glycol, 1, 2-pentanediol, 1, 2-hexanediol, polyethylene glycol-32, dipropylene glycol, sorbitol, 1, 3-propanediol, sucrose.

9. The cosmetic according to claim 5, wherein the humectant is a combination of glycerin, butylene glycol, 1, 2-pentanediol, in a mass ratio of (3-12): (1-5): (1-3).

10. The cosmetic according to claim 5, wherein the skin conditioner is at least one selected from tremella fruit body extract, panthenol, sodium hyaluronate, allantoin, raffinose, trehalose, dipotassium glycyrrhizinate, nicotinamide, adenosine, chitosan, octylglycol, glycereth-26.