CN118526436A - Traditional Chinese medicine amino acid shampoo with alopecia preventing and moisturizing repairing effects and preparation method thereof - Google Patents

Abstract

The invention belongs to the technical field of toiletries, and specifically relates to a Chinese medicine amino acid shampoo with anti-hair loss and moisturizing and repairing effects and a preparation method thereof. The shampoo comprises the following components: a Chinese medicine extract, sodium lauroyl sarcosinate LS-30, sodium methyl cocoyl taurate AK-321, cocamidopropyl betaine CAB-35, decyl glucoside APG 0810, glycerol, 1,3-butylene glycol, D-panthenol, high molecular/low molecular hyaluronic acid, sodium pyrrolidone carboxylate, PEG-120 methyl glucose dioleate, sodium chloride, liquid carbomer SF-1, 2% guar gum hydroxypropyl tripropyl ammonium chloride C-14S, 2% polyquaternium-10JR-400, arginine, proline, disodium ethylenediaminetetraacetic acid and DMDM hydantoin; and the Chinese medicine extract is mainly prepared from Platycladus orientalis leaves, ginger, pine needles and Ligusticum chuanxiong. The Chinese medicinal amino acid shampoo of the present invention can play an anti-hair loss effect, and also has good moisturizing and hair care effects, and is mild, non-irritating and highly safe.

Description

A Chinese medicinal amino acid shampoo with anti-hair loss and moisturizing and repairing effects and its preparation method

Technical Field

The invention belongs to the field of washing products, and particularly relates to a Chinese medicinal amino acid shampoo with anti-hair loss and moisturizing and repairing effects and a preparation method thereof.

Background Art

With the increase of life and work pressure, hair loss has become a worry for most people, and the age of these people is getting younger and younger, which can easily cause physical and psychological burdens to people and affect their daily lives. Traditional Chinese medicine believes that the cause of hair loss is due to dampness, fire, heat and toxins that accumulate and attack the head surface. The second is insufficient qi and blood, which cannot reach the hair mother and blood cannot nourish the hair. Modern medicine believes that high androgen, bacterial inflammation and erosion, and insufficient nutrition of hair are the causes.

As the number of people suffering from hair loss continues to increase, anti-hair loss shampoo has become a new favorite in the market, and the scale of consumption continues to increase. According to data, the market size of China's anti-hair loss product industry has reached 7.636 billion yuan in 2022, of which the market size of daily chemical hair loss products industry is 2.288 billion yuan, accounting for 30% of the entire anti-hair loss market, and the market potential cannot be underestimated. In the context of "appearance economy" and consumption upgrading, the public pays attention to the basic efficacy of shampoo while increasing the demand for "anti-hair loss and hair growth", and also pays more attention to the fine care of hair quality. Consumers' concepts have changed from surface care to deep scalp care.

At present, common anti-hair loss shampoo mainly reduces seborrheic alopecia by removing scalp oil, but it is obvious that its effect is poor, and there is a problem of treating the symptoms but not the root cause. Existing Chinese medicine anti-hair loss and hair growth liquid, hair growth essence and minoxidil tincture are all directly applied or sprayed to the affected area, and there will be aesthetic problems such as yellowing of the head skin after application or spraying, greasy hair, and soft hair style. In addition, people have higher and higher requirements for the safety of the surfactant of shampoo, but the existing shampoo with anionic surfactants such as lauryl sulfate as the main surfactant has certain irritation and excessive degreasing power, which leads to dry hair and increased oil production of the head skin. And amino acid surfactant is a kind of mild surfactant of protein, with low irritation, low toxicity, softness, good biodegradability and good affinity to the human body, etc., which meets the various needs of consumers. Although there are amino acid shampoos on the market, there are few amino acid shampoos with added Chinese medicine ingredients. Therefore, it is of great significance to develop a shampoo containing amino acid surfactants and Chinese herbal extracts, which can safely prevent hair loss, protect hair and nourish hair, and is mild and non-irritating.

Summary of the invention

In view of the problems existing in the prior art, the present invention provides a Chinese medicinal amino acid shampoo with anti-hair loss and moisturizing and repairing effects and a preparation method thereof.

Specifically, the present invention is realized through the following technical solutions:

In the first aspect, the present invention provides a Chinese medicine amino acid shampoo with anti-hair loss and moisturizing and repairing effects, the shampoo comprising the following components in parts by weight: Chinese medicine extract: 1-18 parts, main surfactant sodium lauroyl sarcosinate LS-30: 2-20 parts and sodium methyl cocoyl taurate AK-321: 2-20 parts, auxiliary surfactant cocamidopropyl betaine CAB-35: 2-12 parts and decyl glucoside APG 0810: 1-10 parts, moisturizer glycerol: 1-3 parts, 1,3-butylene glycol: 0.5-2 parts, D-panthenol: 0.1-1 parts, high molecular/low molecular hyaluronic acid: 0.5-2 parts and sodium pyrrolidone carboxylate: 0.1-1 parts, PEG-120 methyl glucose dioleate: 1-5 parts, sodium chloride: 1-4 parts, liquid carbomer SF-1: 0.5-2 parts, 2% guar gum hydroxypropyl tripropyl chloride ammonium chloride C-14S: 0.01-0.1 parts, 2% polyquaternium salt-10JR-400: 0.05-0.15 parts, arginine: 0.1-1 parts, proline: 0.1-1 parts, disodium ethylenediaminetetraacetate: 0.01-0.15 parts, DMDM hydantoin: 0.01-0.15 parts and the balance of deionized water; the Chinese medicine extract is mainly made of raw materials Platycladus orientalis leaves, ginger, pine needles and Chuanxiong.

In a preferred embodiment, in the traditional Chinese medicine extract, the raw materials are composed by weight: 10-40 parts of Platycladus orientalis leaves, 10-40 parts of ginger, 5-20 parts of pine needles, and 5-20 parts of Chuanxiong.

In a preferred embodiment, the shampoo comprises the following components in parts by weight: 8-12 parts of Chinese medicine extract, 12-18 parts of main surfactant sodium lauroyl sarcosinate LS-30 and 2-8 parts of sodium methyl cocoyl taurate AK-321, 4-10 parts of auxiliary surfactant cocamidopropyl betaine CAB-35 and decyl glucoside APG 0810: 2-8 parts, moisturizer glycerol: 1.5-2.5 parts, 1,3-butylene glycol: 0.7-1.3 parts, D-panthenol: 0.2-0.8 parts, high molecular weight/low molecular weight hyaluronic acid: 0.7-1.3 parts and sodium pyrrolidone carboxylate: 0.2-0.8 parts, PEG-120 methyl glucose dioleate: 2-4 parts, sodium chloride: 1.5-2.5 parts, liquid carbomer SF-1: 0.7-1.3 parts, 2% guar gum hydroxypropyl tripropyl ammonium chloride C-14S: 0.02-0.08 parts, 2% polyquaternium-10JR-400: 0.07-0.13 parts, arginine: 0.2-0.8 parts, proline: 0.2-0.8 parts, disodium ethylenediaminetetraacetic acid: 0.03-0.1 parts, DMDM hydantoin: 0.03-0.1 parts and the balance deionized water; in parts by weight, the traditional Chinese medicine extract is mainly prepared from 25-35 parts of raw materials, 25-35 parts of ginger, 10-18 parts of pine needles and 10-18 parts of ligusticum chuanxiong.

In a preferred embodiment, the shampoo comprises the following components by weight: 10 parts of Chinese medicine extract, 15 parts of main surfactant sodium lauroyl sarcosinate LS-30 and 5 parts of sodium methyl cocoyl taurate AK-321, 7 parts of auxiliary surfactant cocamidopropyl betaine CAB-35 and decyl glucoside APG 0810: 5 parts, moisturizing agent glycerol: 2 parts, 1,3-butylene glycol: 1 part, D-panthenol: 0.5 parts, high molecular/low molecular hyaluronic acid: 1 part and sodium pyrrolidone carboxylate: 0.5 parts, PEG-120 methyl glucose dioleate: 3 parts, sodium chloride: 2 parts, liquid carbomer SF-1: 1 part, 2% guar gum hydroxypropyl tripropyl ammonium chloride C-14S: 0.05 parts, 2% polyquaternium salt-10JR-400: 0.1 parts, arginine: 0.5 parts, proline: 0.5 parts, disodium edetate: 0.05 parts, DMDM hydantoin: 0.05 parts and the balance deionized water; in parts by weight, the traditional Chinese medicine extract is mainly made of 30 parts of raw materials, 30 parts of ginger, 15 parts of pine needles and 15 parts of ligusticum chuanxiong.

In a preferred embodiment, the Chinese medicine extract is prepared according to the following preparation method: by weight, 30 parts of Platycladus orientalis leaves, 30 parts of ginger, 15 parts of pine needles, and 15 parts of Chuanxiong are added to a round-bottom flask, 60-80% ethanol solution is added to extract for 1.5-2.5 hours, decocted 1-3 times, and after combining the decoctions, the supernatant is first filtered with a suction device and then centrifuged to obtain the supernatant, which is concentrated and placed in a watch dish, dried to a powder state in a reduced pressure drying oven, and an appropriate amount of powder is taken and prepared into a Chinese medicine extract with 72% ethanol solution.

Preferably, the liquid-to-solid ratio of the Chinese medicinal material to the 60%-80% ethanol solution is 15:1-25:1 mL/g.

In the present invention, the Chinese medicine amino acid shampoo with anti-hair loss and moisturizing and repairing effects, wherein the anti-hair loss effect is played by the Chinese medicine compound composed of Platycladus orientalis, ginger, pine needles, and Chuanxiong, Platycladus orientalis is bitter, astringent, and cold, and enters the lung, liver, and spleen meridians, and has the effects of cooling blood and stopping bleeding, resolving phlegm and relieving cough, and growing hair and black hair, and is compatible with ginger and Chuanxiong to promote blood circulation and qi, promote scalp blood circulation, and nourish hair follicles. Pine needles are bitter and warm in nature, and enter the heart and spleen meridians, and have the effects of promoting blood circulation and relieving itching, can clean the scalp, sterilize and relieve itching, and work together to play an excellent effect. In addition, the above-mentioned Chinese medicine ingredients are found by the inventor after a large number of screenings, and the extract of the above-mentioned Chinese medicine ingredients added to the amino acid shampoo can not only make the shampoo have anti-hair loss and moisturizing and repairing effects, but also can improve the cleaning ability of the shampoo, and achieve unexpected technical effects. In addition, this product contains a variety of moisturizing ingredients such as propylene glycol, 1,3-butylene glycol, D-panthenol, high molecular/low molecular hyaluronic acid and sodium pyrrolidonecarboxylate, which can effectively moisturize the scalp and care for the hair. Even in the autumn and winter, there is no need to reduce the frequency of use or use it with other shampoos with moisturizing effects.

In a second aspect, the present invention provides a method for preparing the Chinese medicinal amino acid shampoo having anti-hair loss and moisturizing and repairing effects as described in the first aspect, the preparation method comprising the following steps:

(1) Preparation of Chinese medicine extract: Take corresponding weight portions of Platycladus orientalis leaves, ginger, pine needles and Chuanxiong rhizome and add them to a round-bottom flask, extract with 72% ethanol solution for 2 hours, decoct twice, combine the decoctions, first filter with a suction filter and then centrifuge to obtain the supernatant, concentrate and place in a watch glass, dry in a vacuum drying oven to a powder state, take an appropriate amount of the powder and use 72% ethanol solution to prepare a Chinese medicine extract;

(2) Preparation of Chinese herbal amino acid shampoo: First, disodium EDTA was dissolved in cold deionized water, and then liquid carbomer SF-1, sodium lauroyl sarcosinate LS-30, cocamidopropyl betaine CAB-35 and decyl glucoside APG0810 were added, and then the mixture was gradually heated to 75°C, and then sodium methyl cocoyl taurate AK-321, 2% guar hydroxypropyl tripropyl ammonium chloride C-14S, 2% polyquaternium-10JR-400, arginine, proline, PEG-120 methyl glucose dioleate and sodium chloride were added in sequence, and the mixture was stirred continuously to dissolve. When the temperature dropped to 45°C, glycerol, 1,3-butylene glycol, D-panthenol, high molecular weight/low molecular weight hyaluronic acid, sodium pyrrolidone carboxylate, DMDM hydantoin were added, and the mixture was kept warm and stirred to obtain the shampoo.

Compared with the prior art, the present invention has the following beneficial effects:

(1) The shampoo disclosed in the present invention is an amino acid type shampoo with an amino acid type surfactant as the main surfactant, and a traditional Chinese medicine anti-hair loss extract is added, which can safely play an anti-hair loss effect, and has the characteristics of moderate cleaning power, mildness and non-irritation, high safety, and easy use.

(2) The shampoo disclosed in the present invention contains specific Chinese herbal extracts, which not only enable the shampoo to prevent hair loss and moisturize and repair hair, but also improve the cleaning ability of the shampoo, achieving unexpected technical effects.

(3) The shampoo disclosed in the present invention contains a variety of moisturizing ingredients, such as glycerol, 1,3-butylene glycol, D-panthenol, high molecular weight/low molecular weight hyaluronic acid and sodium pyrrolidone carboxylate, which can effectively moisturize the hair and head skin, adjust the water-oil balance, and repair dry hair. There is no need to reduce the frequency of use even in autumn and winter, and there is no need to use it with other shampoos with moisturizing effects.

BRIEF DESCRIPTION OF THE DRAWINGS

The accompanying drawings are used to provide a further understanding of the present invention and constitute a part of the specification. Together with the embodiments of the present invention, they are used to explain the present invention but do not constitute a limitation of the present invention. Compared with the control group in the following figure: ^* P<0.05, ^** P<0.01, ^*** P<0.001.

FIG1 is a measurement of the effect of Chinese herbal extracts on the skin color change of the depilatory area of mice;

FIG2 is a measurement of the effect of Chinese herbal extract on the hair growth score and relative area of the depilatory area of mice;

FIG3 is a measurement of the effect of Chinese herbal extracts on the length and weight of new hair in the depilatory area of mice;

FIG4 is a measurement of the effect of Chinese herbal extract on two cytokines, VEGF and TGF-β1, in mouse skin tissue;

FIG5 is a graph showing the water loss rate of the Chinese medicinal amino acid shampoo of the present invention and other shampoos over time.

DETAILED DESCRIPTION

The embodiments of the present invention are described in detail below. The embodiments are given to better illustrate the contents of the present invention and are only used to explain the present invention, but should not be understood as limiting the present invention.

If no specific techniques or conditions are specified in the examples, the techniques or conditions described in the literature in the field or the product instructions are used. If no manufacturer is specified for the reagents or instruments used, they are all conventional products that can be purchased through regular channels.

Example 1: Study on the efficacy of the Chinese herbal extract of the present invention in preventing hair loss

1.1 Preparation of Chinese medicine extracts

Weigh 10g of Platycladus orientalis leaf powder, 10g of ginger powder, 5g of pine needle powder and 5g of Chuanxiong powder into a round-bottom flask, extract with 72% ethanol solution (21 times the mass of the raw materials) for 2 hours, decoct twice, combine the decoctions, filter with a suction device and then centrifuge to obtain the supernatant, and concentrate to obtain a 200mg/mL suspension. Dilute part of the suspension with 72% ethanol solution to 100mg/mL and 50mg/mL, shake well during use, and store in a 4°C refrigerator.

1.2 Experimental animals

Healthy 5-week-old male SPF-grade C57BL/6 mice, weighing 18-22 g, were provided by Liaoning Changsheng Biotechnology Co., Ltd. with a production license number of SCXK(Liao)2020-0001. The mice were bred in the Animal Center of Shenyang Pharmaceutical University with a use license of SYXK(Liao)2021-0009. The breeding conditions were as follows: temperature 20-25°C, relative humidity 45%-55%, 12h alternating lighting, and mice were allowed to eat and drink freely before the experiment was conducted after one week of adaptive breeding.

1.3 Mouse hair removal

Use a pet shaver to shave the hair on both sides of the mouse's back, with an area of 2×3 cm ² , and then evenly apply a small amount of depilatory cream to the depilatory area. After waiting for 30 seconds, gently wash away the remaining hair with warm water. The mouse's skin is pink, and the skin should be kept dry.

1.4 Grouping and Dosing

The mice were randomly divided into 5 groups, 6 in each group, including control group (72% ethanol solution), positive drug group (5% minoxidil tincture), high-dose group of Chinese herbal extract (200 mg/mL), medium-dose group of Chinese herbal extract (100 mg/mL) and low-dose group of Chinese herbal extract (50 mg/mL). All mice were smeared with drugs starting from the first day of the experiment, and smeared continuously for 21 days. They were killed one day after the last administration, 0.5 mL each time, twice a day.

1.5 Pharmacodynamic indicators for anti-hair loss in mice

1.5.1 Observation of skin color in the hair removal area of mice

Starting from the first day of the experiment, the skin color changes of the mice were observed every day. The time when the skin in the hair-depleting area on the back of each mouse changed from pink to gray, the time when the hair began to grow, and the time when the hair completely covered the hair-depleting area were recorded, and photos were taken regularly.

1.5.2 Hair regrowth score and relative area assessment

The new hair of mice was scored on days 12, 15, 18, and 21 after hair removal. The scoring criteria are shown in Table 1. ImageJ was used to perform grayscale analysis of hair growth in the hair removal area on the back of mice and quantify the hair growth score.

Table 1 Hair growth scoring criteria

1.5.3 New hair length and weight

From the time the hair grows in the hair removal area of the mice, the hair is measured every 3 days until the end of the experiment. The mice are selected to pluck hair from 3 locations from head to tail, 5 hairs are plucked from each location, and the length is measured with a ruler and recorded. The average value is taken as the hair length of the mice on that day. After 21 days, the mice are killed by dragging their necks, the fur in the hair removal area is cut off, and a circular skin piece with a diameter of 1 cm is taken from the same hair removal area of each mouse. The hair on the circular skin piece is scraped off with a scalpel and weighed on an analytical balance.

1.5.4 Determination of vascular endothelial growth factor and hair growth inhibitory factor

The hair on the surface of the mouse skin was shaved off, 0.1 g of skin tissue was added to a 2 mL tissue grinding tube, 0.9 mL of PBS was added, and the tube was placed in a tissue grinder for 60 s, centrifuged at 4°C for 15 min, and the supernatant was aspirated into a clean EP tube and stored at -80°C for later use. The levels of VEGF (vascular endothelial growth factor) and TGF-β1 (transforming growth factor β1) in the skin tissue homogenate of each group of mice were determined according to the instructions of the ELISA kit.

1.5.5 Statistical analysis

The experimental data of various indicators were statistically analyzed and processed using GraphPad Prism 9.0 statistical software and Origin 2021 software. The data were measured using mean±SD. The one-way ANOVA method was used to analyze and compare the data among the groups. The differences were considered statistically significant when P<0.05.

1.6 Experimental Results

1.6.1 Effect of Chinese herbal extract on skin color change in the hair removal area of mice

The changes in the hair removal area of mice from the beginning of hair removal to the end of 21 days are shown in Figure 1. As can be seen from Figure 1, after external intervention of hair removal, the skin of mice turned pink, indicating that the hair follicles of mice were in the resting phase at this time. With the beginning of the growth phase, the skin color gradually turned gray, and then the color deepened and short hair began to grow. By taking photos and recording the mice every 3 days, it was found that the minoxidil group, the medium-dose group of traditional Chinese medicine extract, and the high-dose group all had a promoting effect on the growth of mouse hair.

The time when the mice turned from pink to gray, the time when they started to grow hair, and the time when they were completely covered with hair are shown in Table 2 .

Table 2 The time of hair changes in the hair loss area of mice

Note: *, P<0.05 means the difference is relatively significant; **, P<0.01 means the difference is significant; ***, P<0.001 means the difference is extremely significant

As can be seen from Table 2, the skin color of the control group and the low-dose Chinese medicine extract group changed from pink to gray at around 12 days, and the 5% minoxidil group, the medium-dose Chinese medicine extract group and the high-dose Chinese medicine extract group changed color at around 9 days, with a fast growth rate, which was significantly different from the control group. From the time when hair started to grow, it can be seen that the 5% minoxidil group had the fastest hair growth time, followed by the medium-dose Chinese medicine extract group, the high-dose Chinese medicine extract group and the low-dose Chinese medicine extract group, and there were significant differences compared with the control group. The time for hair to be fully covered also confirmed the trend of the previous time change. Therefore, it can be concluded that the anti-hair loss effect is 5% minoxidil group>medium-dose Chinese medicine extract group>high-dose Chinese medicine extract group>low-dose Chinese medicine extract group>control group.

1.6.2 Effect of Chinese herbal extract on hair growth score in the depilatory area of mice

The hair growth scores and relative areas of the depilatory areas of mice treated with Chinese herbal extracts are shown in Figure 2. As can be seen from Figure 2, as the number of days increases, the hair growth scores show a trend of continuous increase. Compared with the control group, the hair growth scores of the 5% minoxidil group, the medium-dose Chinese herbal extract group and the high-dose group are significantly different, and the 5% minoxidil group and the medium-dose Chinese herbal extract group have grown to a level of 3 points since 18 days. It can be seen from the relative area of hair growth in the depilatory area that from the 12th day, the control group has always shown the highest 1-point growth area and 2-point growth area, followed by the low-dose group, high-dose group, medium-dose group and 5% minoxidil group of Chinese herbal extract. The 3-point area of the 5% minoxidil group and the medium-dose group of Chinese herbal extract on the 21st day reached more than 97%, and the 3-point area of the high-dose group of Chinese herbal extract reached 89.2%, which were significantly different from those of the control group. This further illustrates that Chinese herbal extract has a certain promoting effect on hair growth when the concentration reaches 100 mg/mL.

1.6.3 Effects of Chinese herbal extracts on the length and weight of new hair in the depilatory area of mice

The results of the effects of the Chinese herbal extract on the length and weight of new hair in the depilatory area of mice are shown in Table 3 and Figure 3.

Table 3 Determination of the length and weight of new hair in the depilatory area of mice

Note: *, P<0.05 means the difference is relatively significant; **, P<0.01 means the difference is significant; ***, P<0.001 means the difference is extremely significant

As can be seen from Table 3 and Figure 3, at 12d and 15d, compared with the control group, except for the low-dose group of the Chinese medicine extract, the hair length of the 5% minoxidil group and the medium-dose group and high-dose group of the Chinese medicine extract showed significant differences, and there was no significant difference between the medium-dose group and the 5% minoxidil group. At 18d and 21d, there were significant differences in hair length compared with the control group, which shows that the Chinese medicine extract has a certain effect on promoting hair growth; at the same time, from the perspective of hair weight, the hair weight under the same skin area is significantly different from that of the control group, indicating that the Chinese medicine extract has a certain promoting effect on mouse hair growth, and the concentration requirement is basically reached at 100mg/mL.

1.6.4 Effects of Chinese herbal extracts on cytokines in mouse skin tissue

The results of the determination of two cytokines, VEGF and TGF-β1, in mouse skin tissue are shown in Figure 4. As can be seen from Figure 4, compared with the control group, the VEGF levels of the 5% minoxidil group and the low, medium and high dose groups of Chinese herbal extract were significantly increased, indicating that the Chinese herbal extract can significantly promote the hair growth in the depilatory area of mice, but the promoting effect of the Chinese herbal extract is weaker than that of 5% minoxidil; at the TGF-β1 level, compared with the control group, the TGF-β1 levels of the 5% minoxidil group and the low, medium and high dose groups of Chinese herbal extract were significantly reduced, and when the concentration of the Chinese herbal extract was 100 mg/mL, its promoting effect was equivalent to that of 5% minoxidil, and there was no significant difference between the two.

Example 2: Formula composition of the Chinese medicinal amino acid shampoo with anti-hair loss and moisturizing and repairing effects of the present invention

Table 4 Exemplary formula composition of Chinese herbal amino acid shampoo

The above-mentioned traditional Chinese medicine extract was prepared according to the following preparation method: 30 parts of Platycladus orientalis leaves, 30 parts of ginger, 15 parts of pine needles and 15 parts of Chuanxiong were weighed and added into a 2000 mL round-bottom flask, extracted with a 72% ethanol solution of 21 times the mass of the raw materials for 2 hours, decocted twice, and after combining the decoctions, first filtered with a suction filtration device and then centrifuged to obtain the supernatant, which was concentrated and placed in a watch dish, dried to a powder state in a reduced pressure drying oven, and an appropriate amount of powder was taken and used with 72% ethanol solution to prepare an extract containing 50 g of traditional Chinese medicine powder per liter.

Preparation method of Chinese herbal amino acid shampoo: first dissolve disodium edetate with cold deionized water, then add liquid carbomer SF-1, sodium lauroyl sarcosinate LS-30, cocamidopropyl betaine CAB-35 and decyl glucoside APG0810, then gradually heat to 75°C, then add sodium methyl cocoyl taurate AK-321, 2% guar gum hydroxypropyl tripropyl ammonium chloride C-14S, 2% polyquaternium-10JR-400, arginine, proline, PEG-120 methyl glucose dioleate and sodium chloride in sequence, stir continuously to dissolve, add glycerol, 1,3-butylene glycol, D-panthenol, high molecular/low molecular hyaluronic acid, sodium pyrrolidone carboxylate, DMDM hydantoin when the temperature drops to 45°C, and continue to keep warm and stir for 15 minutes.

Example 3: Performance evaluation of the Chinese herbal amino acid shampoo with anti-hair loss and moisturizing and repairing effects of the present invention

3.1 Determination of cleaning ability

Preparation of artificial dirt: Accurately weigh 10.0 g of vegetable oil, 1.6 g of carbon powder, and 0.006 g of liquid paraffin, and stir well.

Decontamination rate test: Weigh the clean hair bundle, apply a certain amount of artificial dirt on the hair bundle, and weigh it. Soak the hair bundle in 25mL of the test solution (20% shampoo solution) and wash it for 2 minutes. Let the hair bundle dry naturally and weigh it. The experiment is repeated 3 times and the average value is taken. Calculate the decontamination rate F% according to formula 1.

Where: M ₁ - weight of clean hair bundle, unit: g; M ₂ - weight of hair bundle after applying oil, unit: g; M ₃ - weight of hair bundle after washing with the test solution, unit: g.

The cleaning ability of the Chinese medicinal amino acid shampoo of the present invention prepared in Example 2 is shown in Table 5.

Table 5 Determination of cleaning rate of sulfate shampoo, amino acid shampoo and commercial shampoo

As can be seen from Table 5, the cleaning rate of the shampoo matrix of the pure sulfate system is 57.57%, which is equivalent to the cleaning power of Shulei shampoo of the commercial sulfate system. After adding 10% of the Chinese medicine extract, the cleaning rate can reach 65.52%, an increase of 7.95%. The cleaning rate of the pure amino acid shampoo matrix is relatively low, only 34.05%. After adding 10% of the Chinese medicine extract, the cleaning rate increases by 13.89%, up to 47.94%. It can be seen that adding 10% of the Chinese medicine extract to the shampoo formula can not only make the shampoo have the effects of preventing hair loss and moisturizing and repairing, but also improve the cleaning ability of the shampoo at the same time, and achieve unexpected technical effects. Although the cleaning power is still slightly weaker than that of the commercially available amino acid shampoo, the Chinese medicine amino acid shampoo of the present invention has a certain cleaning ability, does not remove too much scalp oil, and also has the effects of preventing hair loss and moisturizing and repairing, so it is still a very good choice for consumers who need to wash their hair basically every day.

3.2 Determination of moisturizing properties

Accurately weigh 1.0g of shampoo and apply it evenly on a surface dish, accurately weigh and record, place in a desiccator with a saturated sodium chloride solution to maintain a humidity of 70%, place the desiccator in a 30℃ constant temperature box, accurately weigh at 2, 4, 6, 8, 10, and 12 hours, calculate the water loss rate from the mass difference before and after drying, measure three times in parallel, take the average value, and calculate the water loss rate (S) according to formula 2:

Wherein: m ₀ - weight of shampoo before drying, in grams (g); m ₁ - weight of shampoo after drying for a certain period of time, in grams (g). According to the determination method, the moisturizing properties of sulfate shampoo base, 10% Chinese medicine extract sulfate shampoo, amino acid shampoo base, Chinese medicine amino acid shampoo prepared in Example 2, Shulei shampoo, and Zhiguan shampoo were respectively determined, and the results are shown in FIG5 .

As can be seen from Figure 5, within 12 hours, the water loss rate of the shampoo increases with time. When placed under a constant temperature of 30°C and a humidity of 70% for 12 hours, the amino acid shampoo has the lowest water loss rate, which is 30.51% and 32.27% respectively, which is 19.97% lower than the commercially available amino acid shampoo. The second is sulfate shampoo, which is 36.92% and 35.98%, which is 8.58% lower than the commercially available sulfate shampoo. It can be seen that the Chinese medicine amino acid shampoo of the present invention has better moisturizing ability than commercially available shampoo, helps to lock the moisture of the scalp and hair, and reduces water loss.

3.3 Safety evaluation

3.3.1 Acute eye irritation test

3.3.1.1 Experimental animals

Six healthy adult Japanese white rabbits weighing 1.5-2.0 kg were purchased from the Experimental Animal Center of Shenyang Pharmaceutical University with a production license of SCXK (Liao) 2020-0003. They were kept in the Animal Center of Shenyang Pharmaceutical University with a use license of SYXK (Liao) 2021-0009. The breeding conditions were as follows: temperature 20-25 °C, relative humidity 45-55%, and free access to food and water for experimental animals.

3.3.1.2 Experimental methods and scoring criteria

The lower eyelids of healthy big-eared white rabbits were gently pulled open, and 0.1 mL of traditional Chinese medicine sulfate shampoo, traditional Chinese medicine amino acid shampoo prepared in Example 2 and normal saline were dripped into the left eye of each rabbit, and the rabbit eyelids were forced to close for 1 second, and immediately rinsed with water with sufficient flow for 30 seconds. The other eye was not treated and regarded as a self-control. The eyes of the animals were observed and recorded at 1, 24, 48, and 72 hours, and the scores were given according to Table 6, and the eye irritation reaction was graded according to Table 7.

Table 6 Eye irritation reaction scores

Table 7 Grading of eye irritation reactions

Note: When the scores of cornea, iris and conjunctiva are 0, it can be judged as non-irritating.

3.3.1.3 Acute eye irritation test results

The results of the acute eye irritation test are shown in Tables 8 and 9.

Table 8 Results of acute eye irritation test on traditional Chinese medicine sulfate shampoo

Table 9 Acute eye irritation test results of Chinese medicinal amino acid shampoo of the present invention

As can be seen from Table 8, within 1 hour of eye stimulation, the Chinese medicine sulfate shampoo caused varying degrees of damage to the cornea and conjunctiva of Japanese white rabbits, mainly manifested as corneal opacity and eyelid congestion. After 24 hours of administration, the corneal damage was basically repaired, and after 48 hours of administration, the corneal opacity was completely restored, and only the eyelids had vascular congestion symptoms. The eye damage was completely restored at 72 hours. According to the eye irritation reaction classification in Table 7, the Chinese medicine sulfate shampoo is slightly irritating. As can be seen from Table 9, the Chinese medicine amino acid shampoo of the present invention has slight congestion of conjunctival blood vessels within 1 hour of stimulation, and the eye damage is restored after 48 hours. According to the eye irritation reaction classification in Table 7, the Chinese medicine amino acid shampoo of the present invention is slightly irritating.

3.3.2 Multiple skin irritation tests

3.3.2.1 Experimental animals

Six healthy adult Japanese white rabbits weighing 1.5-2.0 kg were provided by the Animal Center of Shenyang Pharmaceutical University and were adaptively raised in a constant temperature and humidity environment with free access to food and water for 3 days before the experiment.

3.3.2.2 Experimental methods and scoring criteria

The day before application, the hair within the range of 3cm×3cm on both sides of the spine of the Japanese big-eared white rabbit was cut off, and the short hair was removed with a depilatory cream. 0.5g of each of the Chinese medicine sulfate shampoo and the Chinese medicine amino acid shampoo prepared in Example 2 was applied to the left depilated skin, and an equal amount of physiological saline was applied to the right side, and then covered with two layers of gauze and a layer of cellophane, and then fixed with a non-irritating adhesive tape. The application time was 2h, and the residual test substance was removed with warm water after the end. The application time was 14 days. The skin reaction of the application site was observed at 1, 24, 48 and 72h after the test substance was removed, and the score was scored according to the scoring criteria of Table 10. The average score of each rabbit per day was calculated according to Formula 3, and the skin irritation intensity level was judged according to Table 11.

Table 10 Skin irritation reaction scores

Table 11 Skin irritation intensity classification

3.3.2.3 Results of multiple skin irritation tests

The results of multiple skin irritation tests on the traditional Chinese medicine sulfate shampoo and the traditional Chinese medicine amino acid shampoo prepared in Example 2 are shown in Tables 12 and 13.

Table 12 Results of multiple skin irritation tests on traditional Chinese medicine sulfate shampoo

Table 13 Results of multiple skin irritation tests on the Chinese medicinal amino acid shampoo of the present invention

As can be seen from Table 12 and Table 13, one rabbit developed slight erythema on the 10th day after applying the Chinese medicine sulfate shampoo, and no edema was formed until the 14th day. The Chinese medicine amino acid shampoo prepared in Example 2 began to develop slight erythema on the 11th day, and no erythema or edema was formed in the control group. The average score of each Japanese white rabbit per day was calculated from the results: 0.47 points for the Chinese medicine sulfate shampoo and 0.19 points for the Chinese medicine amino acid shampoo prepared in Example 2. This shows that after multiple stimulations, the Chinese medicine amino acid shampoo of the present invention is non-irritating to the skin.

3.3.3 Skin phototoxicity test

3.3.3.1 Experimental animals

Six healthy adult Japanese white rabbits weighing 1.5-2.0 kg were provided by the Animal Center of Shenyang Pharmaceutical University and were adaptively raised in a constant temperature and humidity environment with free access to food and water for 3 days before the experiment.

3.3.3.2 Calculation of irradiation time

A 365nm ultraviolet light source was selected, and the irradiation dose was 10J/ ^cm2 . The light intensity was measured to be 3.6-4.4mW/ ^cm2 . The irradiation time was calculated according to Formula 4, which was 2500s.

Note: 1mW/cm ² = 1mJ/cm ² /sec

3.3.3.3 Preparation of positive drugs

Weigh 12.5 mg of 8-methoxypsoralen in a beaker, dissolve it with a small amount of anhydrous ethanol, and transfer it to a 25 mL volumetric flask to prepare a 0.05% 8-methoxypsoralen solution.

3.3.3.4 Experimental methods and scoring criteria

24 hours before the start of the experiment, the hair on the left and right sides of the back were removed, and 4 hair removal areas were prepared, 2 on each side, and each hair removal area was 2cm×2cm. According to Table 14, 0.2mL of sample was applied to Area 1 and Area 2, and saline was applied to Area 3 and Area 4. After 30 minutes, the two hair removal areas on the left side of the animal were covered with aluminum foil, and the right side was exposed to ultraviolet light for 2500 seconds. The positive control group was treated in the same way. After the end, the skin reaction was observed at 1, 24, 48 and 72 hours, and the score was given according to the scoring criteria in Table 10.

Table 14 Experimental arrangement of animal hair removal area

3.3.3.5 Skin phototoxicity test results

The results of the skin phototoxicity test are shown in Table 15.

Table 15 Skin phototoxicity test results

As can be seen from Table 15, the Chinese medicine sulfate shampoo and the Chinese medicine amino acid shampoo prepared in Example 2 did not form erythema or edema within 72 hours, and 0.05% 8-methoxypsoralen showed a more serious erythema reaction under ultraviolet light, which shows that the Chinese medicine amino acid shampoo of the present invention has no skin phototoxic reaction.

In summary, the Chinese medicinal amino acid shampoo disclosed in the present invention can safely play an anti-hair loss effect, and can also effectively moisturize the hair and head skin, regulate the water and oil balance, and repair dry hair. It has the characteristics of moderate cleaning power, mild and non-irritating, high safety, and easy use.

Obviously, the above embodiments are only examples for clearly explaining the present invention, and are not intended to limit the implementation methods of the present invention. Those skilled in the art may make various changes and modifications to the present invention without departing from the spirit and scope of the present invention. Thus, if these modifications and variations of the present invention fall within the scope of the claims of the present invention and their equivalents, the present invention is also intended to include these modifications and variations.

Claims (6)

1. A traditional Chinese medicine amino acid shampoo with effects of preventing alopecia and moisturizing and repairing is characterized by comprising the following components in parts by weight:

Extracting solution of traditional Chinese medicine: 1-18 parts of main surface active agent sodium lauroyl sarcosinate LS-30:2-20 parts of sodium methyl cocoyl taurate AK-321:2-20 parts of co-surfactant cocamidopropyl betaine CAB-35:2-12 parts of decyl glucoside APG 0810:1-10 parts of humectant glycerol: 1-3 parts of 1, 3-butanediol: 0.5-2 parts of D-panthenol: 0.1-1 part of high polymer/low polymer hyaluronic acid: 0.5-2 parts of sodium pyrrolidone carboxylate: 0.1-1 part of PEG-120 methyl glucose dioleate: 1-5 parts of sodium chloride: 1-4 parts of liquid carbomer SF-1:0.5-2 parts, 2% guar hydroxypropyl tripropyl ammonium chloride C-14S:0.01-0.2 part, 2% polyquaternium-10 JR-400:0.05-0.2 part of arginine: 0.1-1 part of proline: 0.1-1 part of disodium ethylenediamine tetraacetate: 0.01-0.15 part of DMDM hydantoin: 0.01-0.15 part and deionized water balance; the traditional Chinese medicine extract is mainly prepared from raw materials of biota orientalis, ginger, pine needle and ligusticum wallichii.

2. The traditional Chinese medicine amino acid shampoo according to claim 1, wherein in the traditional Chinese medicine extract, the raw materials comprise the following components in parts by weight: 10-40 parts of biota orientalis, 10-40 parts of ginger, 5-20 parts of pine needle and 5-20 parts of ligusticum wallichii.

3. The traditional Chinese medicine amino acid shampoo according to claim 1, wherein the shampoo comprises the following components in parts by weight: extracting solution of traditional Chinese medicine: 8-12 parts of main surface active agent sodium lauroyl sarcosinate LS-30:12-18 parts of sodium methyl cocoyl taurate AK-321:2-8 parts of co-surfactant cocamidopropyl betaine CAB-35:4-10 parts and decyl glucoside APG 0810:2-8 parts of humectant glycerol: 1.5-2.5 parts of 1, 3-butanediol: 0.7-1.3 parts of D-panthenol: 0.2-0.8 part of high molecular/low molecular hyaluronic acid: 0.7-1.3 parts of sodium pyrrolidone carboxylate: 0.2-0.8 part of PEG-120 methyl glucose dioleate: 2-4 parts of sodium chloride: 1.5-2.5 parts of liquid carbomer SF-1:0.7-1.3 parts, 2% guar hydroxypropyl tripropyl ammonium chloride C-14S:0.02-0.08 part, 2% polyquaternium-10 JR-400:0.07-0.13 part of arginine: 0.2-0.8 part of proline: 0.2-0.8 part of disodium ethylenediamine tetraacetate: 0.03-0.1 part of DMDM hydantoin: 0.03-0.1 part and deionized water balance; the traditional Chinese medicine extracting solution is mainly prepared from 25-35 parts of raw materials of cacumen biotae, 25-35 parts of ginger, 10-18 parts of pine needle and 10-18 parts of ligusticum wallichii by weight.

4. The traditional Chinese medicine amino acid shampoo according to claim 1, wherein the shampoo comprises the following components in parts by weight: extracting solution of traditional Chinese medicine: 10 parts of main surface active agent sodium lauroyl sarcosinate LS-30:15 parts of sodium methyl cocoyl taurate AK-321:5 parts of co-surfactant cocamidopropyl betaine CAB-35:7 parts and decyl glucoside APG 0810:5 parts of humectant glycerol: 2 parts of 1, 3-butanediol: 1 part of D-panthenol: 0.5 part of high molecular/low molecular hyaluronic acid: 1 part and sodium pyrrolidone carboxylate: 0.5 part of PEG-120 methyl glucose dioleate: 3 parts of sodium chloride: 2 parts of liquid carbomer SF-1:1 part, 2% guar hydroxypropyl tripropyl ammonium chloride C-14S:0.05 part of 2% polyquaternium-10 JR-400:0.1 part of arginine: 0.5 parts of proline: 0.5 part of disodium ethylenediamine tetraacetate: 0.05 part of DMDM hydantoin: 0.05 parts of deionized water and the balance; the traditional Chinese medicine extracting solution is mainly prepared from the raw materials of 30 parts of biota orientalis, 30 parts of ginger, 15 parts of pine needles and 15 parts of ligusticum wallichii by weight.

5. The traditional Chinese medicine amino acid shampoo according to claim 1, wherein the traditional Chinese medicine extract is prepared according to the following preparation method: taking 30 parts of biota orientalis leaves, 30 parts of ginger, 15 parts of pine needles and 15 parts of ligusticum wallichii, adding the components into a round-bottom flask, adding 60-80% ethanol solution, extracting for 1.5-2.5 hours, decocting for 1-3 times, combining the decoctions, filtering by a suction filtration device, centrifuging, taking supernatant, concentrating, placing into a surface dish, drying in a decompression drying box to obtain powder, and taking a proper amount of powder, and preparing the traditional Chinese medicine extract by using 72% ethanol solution.

6. The method for preparing the traditional Chinese medicine amino acid shampoo with the effects of preventing alopecia and moisturizing and repairing according to any one of claims 1 to 5, which is characterized by comprising the following steps:

(1) Preparing a traditional Chinese medicine extracting solution: adding corresponding parts by weight of biota orientalis leaves, ginger, pine needles and ligusticum wallichii into a round-bottom flask, extracting for 2 hours by using 72% ethanol solution, decocting for 2 times, combining the decoctions, carrying out suction filtration by using a suction filtration device, centrifuging to obtain supernatant, concentrating, placing into a surface dish, drying in a reduced pressure drying oven to obtain powder state, and preparing a proper amount of powder into a traditional Chinese medicine extract by using 72% ethanol solution;

(2) The preparation of the traditional Chinese medicine amino acid shampoo: dissolving disodium ethylenediamine tetraacetate with cold deionized water, adding liquid carbomer SF-1, lauroyl sarcosine sodium LS-30, cocamidopropyl betaine CAB-35 and decyl glucoside APG 0810, gradually heating to 75 ℃, sequentially adding sodium methyl cocoyl taurate AK-321,2% guar hydroxypropyl tripropyl ammonium chloride C-14S,2% polyquaternium-10 JR-400, arginine, proline, PEG-120 methyl glucose dioleate and sodium chloride, continuously stirring and dissolving, cooling to 45 ℃, adding glycerol, 1, 3-butanediol, D-panthenol, high molecular/low molecular hyaluronic acid, sodium pyrrolidone carboxylate and DMDM hydantoin, and continuously preserving heat and stirring.