CN117376787A - An audio-driven microfluidic control system, method and related equipment - Google Patents

Abstract

The invention discloses a micro-fluid control system, a method and related equipment based on audio drive, relates to the technical field of micro-fluid control, and solves the problems of complex structure and high cost of the prior micro-fluid control peripheral, and the technical scheme comprises the following steps: the moving coil is used for converting the electric signal into an acoustic signal; the assembly shell box is matched with the moving coil to form an air cavity, a first air passage which is communicated with the air cavity and the micro-flow control chip is arranged in the assembly shell box, and the number of branches of the first air passage is matched with the number of flow passages in the micro-flow control chip; through combining audio electric signals with micro-fluid control, a special air path structure is formed by adopting a mode of assembling and sealing a moving coil and an assembly housing box, so that the micro-fluid control system based on audio driving is realized, and the micro-fluid control system is simple in structure and low in cost.

Description

An audio-driven microfluidic control system, method and related equipment

Technical field

The present invention relates to the field of microfluidic technology, and more specifically, to an audio-driven microfluidic control system, method and related equipment.

Background technique

As an emerging science and technology, microfluidic technology has been developed for 20 to 30 years. It has been applied in many fields such as chemistry, biology, engineering and physics. It has strong interdisciplinary nature and can control the precision of time, space and analysis objects. It has made breakthroughs in control and can solve many key problems in life analysis. For example, in terms of analysis and detection, microfluidic technology can integrate detection experiments that could only be completed in the laboratory onto a small chip. This not only saves the cost of consumables and time, but more importantly, it can integrate multiple detection methods. technology to improve detection efficiency; in life science research, the development of organ-on-a-chip technology has attracted more and more attention from scientists, because it has broad application prospects in exploring life science research in the future, including the human microenvironment, blood system and The simulation of the lymphatic immune system also provides a more detailed experimental platform for the study of signaling pathways of human biomolecules, making the theoretical basis more sufficient and promoting revolutionary progress in human life science research.

However, although the microfluidic chip itself is small, the external equipment used for microfluidic control is large, bulky, and expensive, which greatly restricts the application scenarios of microfluidic chips. The current existing microfluidic control systems and their existing problems are as follows: the syringe pump system has high cost, large volume, poor portability, and is not suitable for real-time detection environments; the centrifugal drive system has high chip design threshold, weak flexibility, and difficulty in combining temperature Control module; press drive system, which has low accuracy and poor automation; capillary drive system, which has high chip design threshold, low accuracy, and weak fluid control capability; piezoelectric drive system, which requires high voltage and is difficult to be practical.

Based on this, the present invention provides an audio-driven microfluidic control system, method and related equipment to achieve high-precision, low-cost and convenient control of microfluidics.

Contents of the invention

The purpose of this application is to provide an audio-driven microfluidic control system, method and related equipment to solve the problems of complex structure and high cost of existing microfluidic control peripherals. Through the cooperation of the moving coil and the assembly housing box, the Microfluidic control system driven by audio electrical signals.

The first aspect of this application provides a microfluidic control system based on audio drive, including: a moving coil for converting electrical signals into acoustic signals; and an assembly housing box that cooperates with the moving coil to form a gas Cavity, a first air path connecting the air chamber and the microfluidic control chip is provided inside the assembly housing box, and the number of branches of the first air path is adapted to the number of flow channels in the microfluidic chip; Wherein, the air cavity formed by the cooperation between the assembly housing box and the moving coil is used to increase the volume of the air cavity when the moving coil moves in the first direction, and the fluid in the first air path moves toward Move closer to the air chamber; when the moving coil moves in the second direction opposite to the first direction, the volume of the air chamber shrinks, and the fluid in the first air path moves away from the air chamber. direction of the cavity.

Using the above technical solution, the moving coil and the assembly housing box are assembled and sealed to form a special gas circuit structure to realize a microfluidic control system based on audio drive. By changing the waveform, frequency, amplitude and other parameters of the input audio electrical signal, the movement direction and speed of the moving coil are changed, thereby changing the pressure state of the first air path, thereby achieving precise control of the microfluid in the microfluidic chip. , including the direction, flow rate and flow rate of microfluidics. Realize high-precision, low-cost, and convenient control of microfluidics based on audio drive.

In a possible implementation, a second air path connecting the air chamber and the atmosphere is also provided inside the assembly housing box, so that when the moving coil moves in the first direction, the volume of the air chamber increases. When the moving coil moves in the second direction opposite to the first direction, the fluid in the second air path and the fluid in the first air path move together toward the air chamber. , the volume of the air cavity is reduced, and the fluid in the second air path moves away from the air chamber together with the fluid in the first air path.

In a possible implementation, the assembly housing box further includes: a solenoid valve, which is connected to each branch of the first air path and is used to switch on and off each branch of the first air path. Branch circuit; embedded in the surface of the assembly housing box: a slot for inserting the microfluidic chip, a gas port is provided at the bottom of the slot, and the gas port is connected to the branch of the first gas circuit.

In a possible implementation, the assembly housing box includes: a first housing and a second housing that are interconnected and arranged vertically; the surface of the first housing is matingly connected with the moving coil to form a gas A cavity, a first air path connecting the air chamber and extending to the inside of the second housing and a second air path connecting the air chamber and the atmosphere are provided inside the first housing; the surface of the second housing A slot is embedded in the recess. When the microfluidic chip is plugged into the slot, the microfluidic chip is parallel to the first housing. An air port is provided at the bottom of the slot, and the air port is connected to the first housing. The branches of the first air path are connected.

In a possible implementation, the first housing is also provided with a fluorescence excitation module, a heating module and a magnetic bead control module on a surface close to the microfluidic chip; the fluorescence excitation module is used to emit excitation light to excite the microfluidic chip. The fluorescent compound in the fluidic chip generates fluorescent emission light; the temperature control module is used to maintain the set temperature and maintain the sample reaction in the microfluidic chip; the magnetic bead control module is used to control the magnetic field in the microfluidic chip. bead position to achieve sample extraction.

The second aspect of the present application provides an audio-driven microfluidic control method, which is applied to an audio-driven microfluidic control system as described above, including: inputting an electrical signal, and the electrical signal is a positive voltage. Any one or a combination of signals, negative voltage signals, forward sawtooth waves, reverse sawtooth waves, triangle waves, sine waves, square waves, drives the moving coil to move in the first direction or the second direction, Control the movement of fluid in the microfluidic chip; wherein, when there are multiple flow channels in the microfluidic chip, the solenoid valve is used to control the conduction of a single branch of the first air path to control the fluid in a single flow channel in the microfluidic chip. move.

The third aspect of the present application provides a device based on an audio-driven microfluidic control system, including: an audio-driven-based microfluidic control system and a microfluidic chip as described above; wherein, the audio-driven microfluidic control system In the fluid control system, the number of branches of the first gas path is 1; the microfluidic chip includes a sampling hole, a microreactor and a connecting tube that are connected in sequence, and the sampling hole is used to access the fluid to be processed. To measure samples, the microreactor is used to complete various biochemical reactions, and the connecting tube is connected to the first gas path.

The fourth aspect of the present application provides a device based on an audio-driven microfluidic control system, including: the audio-driven-based microfluidic control system and a microfluidic chip as described in any one of the above; wherein the audio-driven microfluidic control system In the fluid control system, the number of branches of the first gas path is 2, including branch A and branch B. The branch A and branch B are controlled on and off by solenoid valves; the microfluidic chip includes : droplet collection cavity, T-shaped droplet generator, oil inlet, water inlet, first connecting pipe and second connecting pipe, the droplet collection cavity is connected with the first end of the T-shaped droplet generator, The second end and the third end of the T-shaped droplet generator are respectively connected with the oil inlet and the water inlet. The oil inlet is connected with the first connecting pipe, and the water inlet is connected with the second connecting pipe. , the first connecting pipe and the second connecting pipe are connected to the branch A and the branch B respectively.

The fifth aspect of the present application provides a device based on an audio-driven microfluidic control system, including: an audio-driven-based microfluidic control system and a microfluidic chip as described in any one of the above; wherein, the audio-driven microfluidic control system In the fluid control system, the number of branches of the first gas path is 2, including branch A and branch B. The branch A and branch B are controlled on and off by solenoid valves; the microfluidic chip includes : detection area, sample, lysis solution, cleaning solution, eluent, micro-column, first connecting tube and second connecting tube, the sample, lysing solution and cleaning solution are merged into the micro-column through their respective pipelines, and the micro-column is One end of the column is connected to the detection area and the second connecting pipe, and the other end of the micro column is connected to the first connecting pipe through the eluent. The first connecting pipe and the second connecting pipe are connected to the branch A and the branch respectively. Road B is connected.

The sixth aspect of this application provides a device based on an audio-driven microfluidic control system, including: the audio-driven-based microfluidic control system and a microfluidic chip as described above; wherein, the audio-driven microfluidic control system In the fluid control system, the number of branches of the first gas path is 2, including branch A and branch B. The branch A and branch B are controlled on and off by solenoid valves; the microfluidic chip includes : a culture organ chamber, a first flow tube, a second flow tube, a conversion center, a first connecting pipe and a second connecting pipe, the upper end of the cultured organ chamber is connected to the upper end of the conversion center through the first flow pipe, and the conversion center The lower end of the center is connected to the lower end of the cultured organ chamber through a second flow tube, and the upper end and lower end of the conversion center are connected to branch A and branch B through first connecting pipes and second connecting pipes respectively.

Compared with the existing technology, this application has the following beneficial effects:

1. The present invention provides an audio-driven microfluidic control system, which assembles and seals the moving coil with a special-structured assembly housing box to form a special air path structure, and realizes audio-electric signal-driven microfluidics at a lower cost; At the same time, it integrates the fluorescence excitation module, heating module and magnetic bead control module, which can accurately complete various microfluidic control requirements required by microfluidic technology;

2. The present invention provides an audio-driven microfluidic control method. The control of the direction, flow rate and flow rate of the microfluid in the chip can be completed through the input of audio electrical signals of different melodies, and can be used to realize sequential liquid sampling and liquid flow control. Various functions such as mixing, liquid circulation, quantitative distribution and droplet generation;

3. The present invention provides related equipment for an audio-driven microfluidic control system. Based on the audio-driven microfluidic control method, the matching gas path structure and microfluidic chip structure are designed, which can be integrated into the chip or become an independent component. It is connected to the chip and can be used repeatedly; the equipment composed of it has the advantages of intelligence, portability, miniaturization and low cost.

Description of the drawings

The drawings described here are used to provide a further understanding of the embodiments of the present invention, constitute a part of this application, and do not constitute a limitation to the embodiments of the present invention. In the attached picture:

Figure 1 is a front view of an audio-driven microfluidic control system provided in Embodiment 1 of the present invention;

Figure 2 is a top view of the audio-driven microfluidic control system provided in Embodiment 1 of the present invention;

Figure 3 is a side view of the audio-driven microfluidic control system provided in Embodiment 1 of the present invention;

Figure 4 is a schematic diagram when the electrical signal provided by Embodiment 2 of the present invention is a forward sawtooth wave and a reverse sawtooth wave;

Figure 5 is a schematic diagram when the electrical signal provided by Embodiment 2 of the present invention is a sine wave;

Figure 6 is a schematic diagram of the electrical signal driving the microfluid to retreat according to Embodiment 2 of the present invention;

Figure 7 is a schematic diagram of electrical signals driving microfluidic advancement provided in Embodiment 2 of the present invention;

Figure 8 is a schematic diagram of the electrical signal control flow channel A provided in Embodiment 2 of the present invention;

Figure 9 is a schematic diagram of the electrical signal control flow channel B provided in Embodiment 2 of the present invention;

Figure 10 is a schematic diagram of an audio-driven microfluidic control system integrated into a chip according to Embodiment 3 of the present invention;

Figure 11 is a schematic diagram of the audio-driven microfluidic control system applied to droplet generation in Embodiment 4 of the present invention;

Figure 12 is a schematic diagram of the application of the audio-driven microfluidic control system provided in Embodiment 5 of the present invention to fully automated nucleic acid extraction and detection;

Figure 13 is a schematic diagram of the application of the audio-driven microfluidic control system provided in Embodiment 6 of the present invention to organ chip research;

Marks and corresponding parts names in the attached drawings:

1. Moving coil; 2. Assembly shell box; 21. First housing; 22. Second housing; 23. Air chamber; 24. First air path; 25. Second air path; 26. Solenoid valve; 27 , slot; 3. Microfluidic chip; 4. Fluorescence excitation module; 5. Heating module; 6. Magnetic bead control module; 71. Injection hole; 72. Microreactor; 73. Connecting tube; 81. Droplet Collection chamber; 82. T-shaped droplet generator; 83. Oil inlet; 84. Water inlet; 91. Detection area; 92. Sample; 93. Lysis solution; 94. Cleaning solution; 95. Eluent; 96. Micro Column; 101, culture organ chamber; 102, first flow tube; 103, second flow tube; 104, conversion center.

Detailed ways

In order to make the purpose, technical solutions and advantages of the present application more clear, the present application will be further described in detail below in conjunction with the embodiments and drawings. The schematic embodiments of the present application and their descriptions are only used to explain the present application and do not As a limitation of this application.

Embodiment 1 provides an audio-driven microfluidic control system, as shown in Figures 1-3. The system includes: a moving coil 1 for converting electrical signals into acoustic signals; and an assembly housing box 2. The housing box 2 cooperates with the moving coil 1 to form an air chamber 23. A first air path 24 is provided inside the assembled housing box 2 to connect the air chamber 23 and the microfluidic control chip 3. The first air path 24 is The number of branches is adapted to the number of flow channels in the microfluidic chip 3;

Among them, the air chamber 23 formed by the cooperation between the assembly housing box 2 and the moving coil 1 is used to increase the volume of the air chamber 23 when the moving coil 1 moves in the first direction, and the first air chamber 23 increases in volume. The fluid in the path 24 moves in the direction closer to the air chamber 23; when the moving coil 1 moves in the second direction opposite to the first direction, the volume of the air chamber 23 shrinks, and the first air chamber 23 The fluid in the path 24 moves away from the air chamber 23 .

Specifically, the moving coil 1 is used to convert audio electrical signals into vibrating sound signals. For example, existing speakers, horns and other devices can be used, or other self-made structures capable of converting audio electrical signals into vibrating sound signals; assemble the shell The box 2 is a shell structure, in which air paths can be set and various devices can be fixed; the assembly shell box 2 is assembled with the moving coil 1, and a cavity is formed between the two, which is called the air cavity 23. In order to ensure the airtightness of the connection between the assembly housing box 2 and the moving coil 1, sealing methods such as compression gaskets, sealants, and O-rings can be used to prevent fluid from seeping from the connection and create a sealed air cavity 23 environment. Furthermore, in order to realize the electrical signal driving microfluid, this system is provided with a first air path 24 that connects the air chamber 23 and the microfluidic control chip; the microfluidic chip 3 can choose a common microfluidic chip 3 controlled by air pressure, in which It contains a flow channel, and the opening of the flow channel is connected with the first air path 24 to realize the communication between the first air path 24 and the flow channel. The purpose of this system is to control the movement of the microfluid in the microfluidic chip 3, specifically by changing the air pressure in the air chamber 23 through the action of the moving coil 1, and adjusting the movement of the fluid in the microfluidic chip 3 through the first air path 24.

See Figure 6-7. The principle of this system to drive microfluidics through audio electrical signals is as follows: the audio electrical signals drive the moving coil 1 to vibrate, and the vibration of the moving coil 1 changes the pressure state in the air chamber 23, and the pressure state in the first air path 24 changes accordingly, and then Control the movement of the microfluid in the microfluidic chip 3 connected to it. Taking the system placed horizontally as an example, due to the influence of the audio electrical signal, the moving coil 1 may move upward or downward. When the moving coil 1 moves upward, the volume of the air chamber 23 increases, and the first air path 24 generates a flow toward the air chamber 23 The pressure of the movement in the direction of the microfluidic chip 3 enables the microflow in the microfluidic chip 3 to move toward the opening of the flow channel. When the moving coil 1 moves downward, the volume of the air chamber 23 decreases, and the first air path 24 moves away from the air chamber 23. pressure, thereby realizing the movement of the microflow in the microfluidic chip 3 away from the opening of the flow channel.

It should be noted that the present invention combines audio electrical signals with microfluidic control, assembles and seals the moving coil 1 and the assembly housing box 2, forms a special gas circuit structure, and realizes a microfluidic control system based on audio drive. By changing the waveform, frequency, amplitude and other parameters of the input audio electrical signal, the movement direction and speed of the moving coil 1 are changed, thereby changing the pressure state of the first air path 24, thereby realizing the microfluidic control in the microfluidic chip 3. Precise control of microfluidic direction, flow rate and flow rate. Microfluidic control can be realized through the moving coil 1 and the assembled housing box 2. The overall structure is portable, low-cost and has high control accuracy. It can be widely used in microfluidic chips 3 controlled by air pressure to realize microfluidic chips. 3. Required functions such as liquid sequential injection, liquid mixing, liquid circulation, quantitative distribution, and droplet generation.

In a possible implementation, a second air passage 25 connecting the air chamber 23 and the atmosphere is also provided inside the assembly housing box 2 so that when the moving coil 1 moves in the first direction, the second air path 25 is provided. The volume of the air chamber 23 increases, and the fluid in the second air path 25 and the fluid in the first air path 24 move toward the direction of the air chamber 23 together; when the moving coil 1 moves toward the air chamber 23 When moving in the second direction opposite to the first direction, the volume of the air chamber 23 is reduced, and the fluid in the second air path 25 and the fluid in the first air path 24 move away from the air chamber 23 together. move.

The reason for providing the second air path 25 is that when a sawtooth wave is used for fluid control, the second air path can be used for exhaust, and when a square wave or sine wave is used for fluid control, the second air path can be blocked. Only the first gas path is used to control fluid movement within the microfluidic chip.

In a possible implementation, the assembly housing box 2 also includes: a solenoid valve 26 connected to each branch of the first air path 24 for switching on and off the first gas path. Each branch of the air path 24.

Specifically, when there are multiple flow channels in the microfluidic chip 3 and it is necessary to control the microfluids in the multiple flow channels respectively, the first gas path 24 needs to have branches corresponding to the number of flow channels, and each branch passes through the electromagnetic Valve 26 controls the gating. For example, when the microfluidic chip 3 contains two flow channels and has two flow channel openings, the first gas path 24 in the system needs to be provided with two branches, and the two branches are correspondingly connected to the two flow channel openings to achieve two The branches are connected to the corresponding ones of the two flow channels; when it is necessary to control the microfluid in flow channel A, just open the solenoid valve 26 corresponding to branch A.

Preferably, the first gas path 24 in this system can adopt a branched structure to realize the control of multiple flow channels, that is, one end of the first gas path 24 close to the air chamber 23 is the main channel, and the end close to the microfluidic chip 3 is based on the microfluidic flow. The number of flow channels in the control chip 3 is divided into multiple branches, and each branch is controlled by a solenoid valve 26.

Optionally, multiple first gas channels 24 are provided, and their number is determined according to the number of flow channels in the microfluidic chip 3 . One end of the plurality of first air passages 24 is connected to the air chamber 23, and the other end is connected to the corresponding flow channel opening of the microfluidic chip 3 to realize communication between the air chamber 23 and the flow channel. Each first air path 24 is controlled by a solenoid valve 26.

It should be noted that the solenoid valve 26 may be a single solenoid valve 26 integrating multiple channels or a plurality of independent solenoid valves 26 .

Further, the assembly housing box 2 is embedded with a slot 27 for inserting the microfluidic chip 3 on the surface. An air port is provided at the bottom of the slot 27. The branch of the air port and the first air path 24 is Connected.

Specifically, in order to fix the microfluidic chip 3 and ensure that the flow channel in the microfluidic chip 3 is connected with the first air path 24 of the system, a slot 27 is embedded on the surface of the assembly housing box 2, and the bottom of the slot 27 An air port connected to the branch of the first air path 24 is provided.

In a possible implementation, the assembly housing box 2 includes: a first housing 21 and a second housing 22 that are interconnected and arranged vertically; the surface of the first housing 21 is in contact with the moving coil 1 Fittingly connected to form an air chamber 23, a second air path 25 connecting the air chamber 23 and the atmosphere is provided inside the first housing 21, and a first air path 25 connecting the air chamber 23 and extending to the inside of the second housing 22 is provided. Air path 24; the recessed surface of the second housing 22 is embedded with a slot 27. When the microfluidic chip 3 is inserted into the slot 27, the microfluidic chip 3 and the first The housing 21 is parallel, and an air port is provided at the bottom of the slot 27 . The air port is connected to the branch of the first air path 24 .

Specifically, this embodiment exemplifies a possible implementation structure of assembling the housing box 2, as shown in FIG. 3, including a first housing 21 and a second housing 22 that are connected to each other and arranged vertically. In Figure 3, the first housing 21 is arranged horizontally, with the moving coil 1 connected to it, and the second housing 22 is arranged vertically, with a recessed side wall embedded in a slot 27. When the microfluidic chip 3 is inserted into the slot 27, the microfluidic chip 3 is inserted into the slot 27. The fluidic chip 3 is parallel to the first housing 21 . The structure is simple and easy to implement.

It should be noted that when the moving coil 1 is a quasi-conical structure as shown in Figure 3, the conical structure itself has a certain space inside, so it can be directly connected to the horizontally arranged first housing 21 to naturally form an air cavity. twenty three. If the moving coil 1 itself does not have space or the space is too small, a part of the surface of the first housing 21 can be recessed inward, and the recessed portion cooperates with the moving coil 1 to form an air cavity 23 .

In a possible implementation, the first housing 21 is also provided with a fluorescence excitation module 4, a heating module 5 and a magnetic bead control module 6 on the surface close to the microfluidic chip 3; the fluorescence excitation module 4 is used for Emit excitation light to excite the fluorescent compound in the microfluidic chip 3 to produce fluorescent emission light; the temperature control module is used to maintain the set temperature and maintain the reaction of the sample 92 in the microfluidic chip 3; the magnetic bead control module 6 Used to control the position of the magnetic beads in the microfluidic chip 3 to achieve sample 92 extraction.

Specifically, in order to expand the application scenarios of the system, the fluorescence excitation module 4, the heating module 5 and the magnetic bead control module 6 can be disposed on the surface of the first housing 21 close to the microfluidic chip 3. The fluorescence excitation module 4 is used to emit excitation light, which causes the fluorescent compound to be detected to emit fluorescent emission light; the temperature control module can maintain the temperature to a specific value, so that the sample 92 undergoes corresponding reactions, such as nucleic acid amplification; the magnetic bead control module 6. Magnetic beads can be fixed and nucleic acids extracted.

It should be noted that the above content is only an exemplary expansion and does not constitute a restriction on this system. This system can add or delete some existing modules according to actual needs to meet the common functions of microfluidic technology.

It can be understood that the music-driven microfluidic control system provided in this example has the characteristics of portability, low cost, and high control accuracy. At the same time, the fluorescence excitation module 4, the heating module 5 and the magnetic bead control module 6 are integrated, which can accurately complete various microfluidic control requirements required by microfluidic technology, including fluid direction, flow rate and flow rate, etc., to achieve sequential liquid flow. It has functions such as quantitative distribution of samples and samples 92, fluid mixing, liquid circulation, droplet production, and nucleic acid amplification.

Embodiment 2 provides an audio-driven microfluidic control method, as shown in Figures 4-9, applied to the audio-driven microfluidic control system as described above. The method includes: inputting an electrical signal, The electrical signal is any one or a combination of positive voltage signal, negative voltage signal, forward sawtooth wave, reverse sawtooth wave, triangle wave, sine wave, and square wave, driving the moving coil 1 to the first direction. The movement in one direction or the second direction controls the movement of fluid in the microfluidic chip 3;

When there are multiple flow channels in the microfluidic chip 3 , the solenoid valve 26 is used to control the branch of the first gas channel 24 to be individually conductive to control the movement of fluid in a single flow channel in the microfluidic chip 3 .

Specifically, please refer to Figure 6-7. When the input electrical signal is a negative voltage, the moving coil 1 shrinks upward, sucking the fluid to flow backward; when the input electrical signal is a positive voltage, the moving coil 1 presses downward. , pushing the fluid forward.

When different waveforms are given, the movement of the fluid will also be different. When a forward sawtooth wave is used to drive the moving coil 1, the forward force is greater than the backward force, and the fluid advances forward; when a reverse sawtooth wave is used to drive the moving coil 1 When driving, the backward force is greater than the forward force, and the fluid retreats; when using a triangle wave, the fluid continuously advances first and then retreats. At this time, the microfluid in the microfluidic chip 3 can be mixed in a mixing chamber with a special structure, such as a The mixing chamber of the fish scale-shaped channel is continuously mixed; when a sine wave is used to drive the moving coil 1, a one-way valve can be integrated on the flow channel in the microfluidic chip 3, so that the liquid can flow on the microfluidic chip 3 in a single direction. At this time, the movement of the moving coil 1 changes slowly, and the fluid flow is also gentle, which is suitable for samples such as cells that are sensitive to the fluid flow speed. When square waves are used, the driving force is large and the fluid movement speed is fast.

Referring to Figures 8-9, when there are multiple flow channels in the microfluidic chip 3, the moving coil 1 cooperates with the solenoid valve 26 to control the movement of fluid in a single flow channel in the microfluidic chip 3. When the moving coil 1 is pressed down, the solenoid valve 26 opens the A channel. When the moving coil 1 is retracted, the solenoid valve 26 opens the B channel, so that each one-way flow can be achieved in the A and B channels.

This example also provides an exemplary schematic diagram when the electrical signal is a forward sawtooth wave, a reverse sawtooth wave, and a sine wave, as shown in Figure 4-5.

It should be noted that by changing the waveform, frequency, amplitude and other parameters of the input audio electrical signal, the movement direction and speed of the moving coil 1 can be changed, thereby changing the pressure state of the first air path 24, thereby realizing microfluidic control. The microfluid in chip 3 is precisely controlled, including the direction, flow rate and flow rate of the microfluid.

Embodiment 3 provides a device based on an audio-driven microfluidic control system, as shown in Figure 10 , which is a schematic diagram of an audio-driven microfluidic control system integrated in a chip (audio-driven microfluidic control system shown briefly). The equipment includes: the audio-driven microfluidic control system and microfluidic chip 3 described in Embodiment 1;

Wherein, in the audio-driven microfluidic control system, the number of branches of the first gas path 24 is 1; the microfluidic chip 3 includes sequentially connected: sampling holes 71, microreactors 72 and The connecting pipe 73 , the sampling hole 71 is used to access the sample 92 to be tested, the microreactor 72 is used to complete various biochemical reactions, the connecting pipe 73 is connected with the first gas path 24 .

Specifically, the audio-driven microfluidic control system can be integrated with the chip as a micropump to control the microfluid. The equipment mainly includes a sampling hole 71, a microreactor 72, and a micropump. The required reagents can be added to the sampling hole 71, and the micropump can provide negative pressure power to guide the liquid into the microreactor 72. Various biochemical reactions can be completed through the design in the microreactor 72. The micropump can provide all the necessary reagents. Functional requirements such as microfluidic mixing, microfluidic direction change, and flow rate change are required.

Embodiment 4 provides a device based on an audio-driven microfluidic control system. Please refer to FIG. 11 at any time. FIG. 11 is a schematic diagram of the audio-driven microfluidic control system applied to droplet generation (wherein, the audio-driven microfluidic control system The control system is briefly shown). The equipment includes: the audio-driven microfluidic control system and microfluidic chip 3 as described in Example 1;

Wherein, in the audio-driven microfluidic control system, the number of branches of the first gas path 24 is 2, including branch A and branch B, and the branch A and branch B pass through the solenoid valve 26 Control on-off; the microfluidic chip 3 includes: a droplet collection chamber 81, a T-shaped droplet generator 82, an oil inlet 83, a water inlet 84, a first connecting pipe and a second connecting pipe. The cavity 81 is connected to the first end of the T-shaped droplet generator 82, and the second end and the third end of the T-shaped droplet generator 82 are connected to the oil inlet 83 and the water inlet 84 respectively. The oil inlet 83 is connected to the first connecting pipe, the water inlet 84 is connected to the second connecting pipe, and the first connecting pipe and the second connecting pipe are connected to the branch A and the branch B respectively.

Specifically, the audio-driven microfluidic control system can be reused as an off-chip micropump. Simply connect the first air path 24 to the corresponding inlet of the chip through a connecting tube to complete the microfluidic control within the chip. High portability, reusable, low cost and other advantages. The overall equipment includes a droplet collection chamber 81, a T-shaped droplet generator 82, an oil inlet 83, a water inlet 84, connecting pipes and a micropump. Through the switching of the solenoid valve 26, a part of the water is first pushed, the water inlet 84 is closed, the oil inlet 83 is connected, a part of the oil is pushed, and multiple droplets can be quickly generated by continuous switching. At the same time, the droplets can be controlled by the frequency of the audio signal. The size of the droplets is also limited by the channel, so the size of the microdroplets can be converted from nanoliter to milliliter levels. For example, when the T-shaped droplet generator 82 is at the nanometer level, it can generate nanoliter droplets, and when it is at the millimeter level, it can produce microliter droplets. At the same time, the generation of droplets is not affected by the initial flow rate adjustment, which can avoid the loss of reagents or the generation of unevenly sized droplets when the flow rate is first adjusted, because the droplet size can be precisely controlled by frequency.

Embodiment 5 provides a device based on an audio-driven microfluidic control system, as shown in Figure 12. Figure 12 is a schematic diagram of an audio-driven microfluidic control system applied to fully automated nucleic acid extraction and detection (wherein, the audio-based microfluidic control system The driven microfluidic control system is briefly shown). The equipment includes: the audio-driven microfluidic control system and microfluidic chip 3 as described in Example 1;

Wherein, in the audio-driven microfluidic control system, the number of branches of the first gas path 24 is 2, including branch A and branch B, and the branch A and branch B pass through the solenoid valve 26 Control on-off; the microfluidic chip 3 includes: detection area 91, sample 92, lysis solution 93, cleaning solution 94, eluent 95, micro-column 96, first connecting tube and second connecting tube, the sample 92. The lysis solution 93 and the cleaning solution 94 are merged into the micro-column 96 through their respective pipelines. One end of the micro-column 96 is connected to the detection area 91 and the second connecting tube, and the other end of the micro-column 96 is connected to the first connecting pipe through the eluent 95. Connecting pipes, the first connecting pipe and the second connecting pipe are connected to the branch A and the branch B respectively.

Specifically, the audio-driven microfluidic control system can be used as a micropump, connected to an adapted nucleic acid extraction and detection chip, and the entire process of automated nucleic acid extraction and digital detection can be completed through the microfluidic control system. The equipment includes a detection area 91, a sample 92, a lysis solution 93, a cleaning solution 94, an eluent 95, a micro-column 96, connecting tubes and a micro-pump. The lysis solution 93, the cleaning solution 94 and the eluent 95 can be packaged in the chip in advance. When the sample 92 is added, branch B provides negative pressure to simultaneously drain the sample 92 and the lysis solution 93 into the micro-column 96, and through the audio The control of the signal frequency completes the back-and-forth mixing of the sample 92 solution and the lysis solution 93 on the micro-column 96. After the nucleic acid adsorption is completed, the liquid is drained away; at the same time, the cleaning solution 94 is opened, and the excess impurities on the micro-column 96 are removed through the same operation. Clean, then close branch B, connect branch A, inject the eluent 95 into the micro-column 96, clean and mix the nucleic acid, and finally provide positive pressure to inject the mixed nucleic acid solution into the detection area 91. The detection area 91 can be designed Multiple microwell arrays are driven by liquid to complete quantitative distribution and are finally sealed to complete digital detection of nucleic acids.

Embodiment 6 provides a device based on an audio-driven microfluidic control system, as shown in Figure 13. Figure 13 is a schematic diagram of an audio-driven microfluidic control system applied to organ chip research (wherein, the audio-driven microfluidic control system The fluid control system is briefly shown). The equipment includes: the audio-driven microfluidic control system and microfluidic chip 3 as described in Example 1;

Wherein, in the audio-driven microfluidic control system, the number of branches of the first gas path 24 is 2, including branch A and branch B, and the branch A and branch B pass through the solenoid valve 26 Control on-off; the microfluidic chip 3 includes: a culture organ chamber 101, a first flow tube 102, a second flow tube 103, a conversion center, a first connection tube and a second connection tube. The culture organ chamber 101 The upper end of the conversion center 104 is connected to the upper end of the conversion center 104 through the first flow tube 102, and the lower end of the conversion center 104 is connected to the lower end of the cultured organ chamber 101 through the second flow tube 103. The upper end and the lower end of the conversion center 104 are respectively It is connected to branch A and branch B through the first connecting pipe and the second connecting pipe.

Specifically, the audio-driven microfluidic control system can be connected to an adapter chip, and the combined device can well simulate the human vascular microenvironment, complete the flow replacement of the culture medium, and provide a fluid system similar to the human heart. The equipment includes a culture organ chamber 101, a first flow tube 102, a second flow tube 103, a conversion center 104, connecting tubes and a micropump. The conversion center 104 can transport fresh special culture medium to the organ culture chamber at a certain flow rate. At the same time, it can recover the culture medium that has been used by organ tissues. The metabolites in the recovered culture medium can be analyzed and detected to further understand the organs. The function and physiological and biochemical status of the tissue, while getting rid of the long residual time of metabolites caused by conventional static organ culture, which has an impact on the organ tissue, ensuring the reliability of the final analysis results.

The above-described specific embodiments further describe the objectives, technical solutions and beneficial effects of the present invention in detail. It should be understood that the above-mentioned are only specific embodiments of the present invention and are not intended to limit the scope of the present invention. Any modifications, equivalent substitutions, improvements, etc. made within the spirit and principles of the present invention shall be included in the protection scope of the present invention.

Claims (10)

1. An audio drive-based microfluidic control system, comprising:

the moving coil is used for converting the electric signal into an acoustic signal; and, a step of, in the first embodiment,

the assembling shell box is matched with the moving coil to form an air cavity, a first air passage which is communicated with the air cavity and the micro-flow control chip is arranged in the assembling shell box, and the number of branches of the first air passage is matched with the number of flow passages in the micro-flow control chip;

the air cavity is formed by the assembly shell box and the moving coil in a matching mode, and is used for increasing the volume of the air cavity when the moving coil moves towards a first direction, and fluid in the first air channel moves towards a direction close to the air cavity; when the moving coil moves in a second direction opposite to the first direction, the volume of the air cavity is reduced, and the fluid in the first air passage moves in a direction away from the air cavity.

2. The audio-drive-based micro-fluid control system according to claim 1, wherein a second air channel which is communicated with the air cavity and the atmosphere is further arranged in the assembled housing box, and is used for increasing the volume of the air cavity when the moving coil moves towards a first direction, and the fluid in the second air channel and the fluid in the first air channel move together towards a direction close to the air cavity; when the moving coil moves in a second direction opposite to the first direction, the volume of the air cavity is reduced, and the fluid in the second air channel and the fluid in the first air channel move together in a direction away from the air cavity.

3. The audio drive-based microfluidic control system of claim 1, wherein the assembled housing box interior further comprises: the electromagnetic valve is connected with each branch of the first air passage and used for switching on and off each branch of the first air passage;

the surface of the assembled shell box is embedded with: the slot is used for inserting the microfluidic chip, the bottom of the slot is provided with an air port, and the air port is communicated with the branch of the first air path.

4. A microfluidic control system based on audio actuation according to any of claims 2-3, wherein the assembled housing box comprises: a first housing and a second housing which are communicated with each other and are vertically arranged;

the surface of the first shell is connected with the moving coil in a matching way to form an air cavity, and a first air channel communicated with the air cavity and extending to the inside of the second shell and a second air channel communicated with the air cavity and the atmosphere are arranged in the first shell;

the surface of the second shell is concavely embedded with a slot, when the microfluidic chip is inserted into the slot, the microfluidic chip is parallel to the first shell, an air port is arranged at the bottom of the slot, and the air port is communicated with a branch of the first air path.

5. The audio-drive-based micro-fluidic control system according to claim 4, wherein the surface of the first housing, which is close to the micro-fluidic chip, is further provided with a fluorescence excitation module, a heating module and a magnetic bead control module;

the fluorescence excitation module is used for emitting excitation light and exciting fluorescent compounds in the microfluidic chip to generate fluorescence emission light;

the temperature control module is used for maintaining a set temperature and sample reaction in the microfluidic chip;

the magnetic bead control module is used for controlling the positions of magnetic beads in the microfluidic chip to realize sample extraction.

6. An audio-drive-based micro-fluidic control method applied to the audio-drive-based micro-fluidic control system according to any one of claims 1 to 5, comprising:

inputting an electric signal, wherein the electric signal is any one or a combination of a positive voltage signal, a negative voltage signal, a positive sawtooth wave, a reverse sawtooth wave, a triangular wave, a sine wave and a square wave, and driving the moving coil to move in a first direction or a second direction so as to control the movement of fluid in the microfluidic chip;

when a plurality of flow channels exist in the microfluidic chip, the electromagnetic valve controls the conduction of a single branch of the first air channel, and controls the fluid movement of the single flow channel in the microfluidic chip.

7. An apparatus for an audio-driven based microfluidic control system, comprising: an audio drive based microfluidic control system and microfluidic chip according to any of claims 1-5;

in the micro-fluid control system based on audio driving, the number of branches of the first air circuit is 1;

the microfluidic chip comprises the following components which are communicated in sequence: the micro-reactor is used for completing various biochemical reactions, and the connecting pipe is communicated with the first air path.

8. An apparatus for an audio-driven based microfluidic control system, comprising: an audio drive based microfluidic control system and microfluidic chip according to any of claims 1-5;

in the micro-fluid control system based on audio driving, the number of branches of the first air circuit is 2, and the micro-fluid control system comprises a branch A and a branch B, wherein the branch A and the branch B are controlled to be on-off through electromagnetic valves;

the microfluidic chip includes: the liquid drop collecting device comprises a liquid drop collecting cavity, a T-shaped liquid drop generator, an oil inlet, a water inlet, a first connecting pipe and a second connecting pipe, wherein the liquid drop collecting cavity is communicated with the first end of the T-shaped liquid drop generator, the second end and the third end of the T-shaped liquid drop generator are respectively communicated with the oil inlet and the water inlet, the oil inlet is communicated with the first connecting pipe, the water inlet is communicated with the second connecting pipe, and the first connecting pipe and the second connecting pipe are respectively communicated with the branch A and the branch B.

9. An apparatus for an audio-driven based microfluidic control system, comprising: an audio drive based microfluidic control system and microfluidic chip according to any of claims 1-5;

in the micro-fluid control system based on audio driving, the number of branches of the first air circuit is 2, and the micro-fluid control system comprises a branch A and a branch B, wherein the branch A and the branch B are controlled to be on-off through electromagnetic valves;

the microfluidic chip includes: the detection device comprises a detection area, a sample, a lysate, a cleaning solution, an eluent, a microcolumn, a first connecting pipe and a second connecting pipe, wherein the sample, the lysate and the cleaning solution are converged into the microcolumn through respective pipelines, one end of the microcolumn is communicated with the detection area and the second connecting pipe, the other end of the microcolumn is communicated with the first connecting pipe through the eluent, and the first connecting pipe and the second connecting pipe are respectively communicated with the branch A and the branch B.

10. An apparatus for an audio-driven based microfluidic control system, comprising: an audio drive based microfluidic control system and microfluidic chip according to any of claims 1-5;

in the micro-fluid control system based on audio driving, the number of branches of the first air circuit is 2, and the micro-fluid control system comprises a branch A and a branch B, wherein the branch A and the branch B are controlled to be on-off through electromagnetic valves;

the microfluidic chip includes: the culture organ comprises a culture organ chamber, a first flow pipe, a second flow pipe, a switching center, a first connecting pipe and a second connecting pipe, wherein the upper end of the culture organ chamber is communicated with the upper end of the switching center through the first flow pipe, the lower end of the switching center is communicated with the lower end of the culture organ chamber through the second flow pipe, and the upper end and the lower end of the switching center are respectively communicated with the branch A and the branch B through the first connecting pipe and the second connecting pipe.