CN1170578A

CN1170578A - Dilong Capsules

Info

Publication number: CN1170578A
Application number: CN96118724A
Authority: CN
Inventors: 王克为
Original assignee: Fourth Military Medical University FMMU
Current assignee: Air Force Medical University
Priority date: 1996-07-17
Filing date: 1996-07-17
Publication date: 1998-01-21

Abstract

Earthworm capsule relates to an active substance-containing active substance product with anticancer function, which is prepared by washing fresh live earthworms with water, homogenating, freezing, thawing, filtering, freeze-drying and encapsulating. According to the determination, the earthworm capsule contains protein, a plurality of amino acids, riboflavin, vitamin C, DNA, RNA, reducing sugar, trace elements and the like. Toxicity tests prove that the Chinese medicinal composition has no toxic or side effect. Through clinical observation, the partial remission rate of 625 cases of late-stage esophageal cancer is 45 percent, the complete remission rate of the advanced-stage esophageal cancer combined with radiotherapy is 33 percent, the curative effect is obvious, and the effective rate of inhibiting lung cancer is 79.2 percent when the advanced-stage esophageal cancer combined with chemotherapy has obvious synergistic effect.

Description

Earthworm capsule

The present invention relates to a kind of pharmaceutical products that contains anti-cancer active matter.

The traditional Chinese medical science thinks that the formation of tumor at first is positive QI-insufficiency, and it is poly-that zang-fu disharmony and cause qi depression to blood stasis, expectorant coagulate poison, and scorchingly hot penting up forms lump, name day " cancer ", " rock ".Though Chinese traditional treatment tumor rule is many, conclude not outer four big rules: method of dissipating heat and detoxifying, hard masses softening and resolving method, activating blood and removing stasis Method and the body resistance strengthening and constitution consolidating method.By blood removing blood stasis with potent drugs soup " the bright experience collection that borders in the south of Zhao " is one of famous anticancer prescription of blood-activating stasis-removing kind.

Form by Hirudo, worm, Pheretima, Semen Pharbitidis, Fructus Liquidambaris, Herba speranskiae tuberculatae, Fructus Polygoni Orientalis, Radix seu Herba Spiranthis Lanceae, Radix Arnebiae (Radix Lithospermi), Tabanus ten flavor Chinese medicines by blood removing blood stasis with potent drugs soup.

The Japan scholar helps the paper that the clear Yan Shi of rattan delivers at him: Chinese prescription research, 1979.2P51 described in: when the research Chinese medicine medicine for preventing, find, Pheretima through decoct, filtration, distilling under reduced pressure and the dry mixture that forms, be 50% to 70% to the suppression ratio of S180 transplanted tumor.

Also propose Pheretima in " clinical efficacy of Chinese medicine medicine for preventing " book of Qian Baiwen work and can be used for treating intracranial tumour (see Qian Baiwen: the clinical efficacy of Chinese medicine medicine for preventing, 113, Shanghai, Shanghai translation issuing company, 1986.1).

Lumbricus is referred to as Pheretima at Chinese medicine, therefore generally not containing poisonous composition, study on its antitumaous effect basis Pheretima as anticancer drug of first choice forefathers, is prerequisite not destroy its active substance, designed the extracting method of working substance, prepared PTS---earthworm capsule.

The objective of the invention is on the basis of forefathers research, go out pure Chinese medicine cancer therapy drug---earthworm capsule with the prepared of uniqueness.This Pheretima capsule contains protein, several amino acids, riboflavin, vitamin C, DNA and RNA, reducing sugar and trace element etc. after measured.Have no side effect through the toxicity test proof.Part remission rate through clinical observation 625 routine late esophagus cancers is 45%; Share with chemotherapeutic, the effective percentage 79.2% that suppresses pulmonary carcinoma has remarkable potentiation; With radiotherapy and usefulness, be 33% to the complete remission rate of the pulmonary carcinoma and the esophageal carcinoma, remarkable potentiation is arranged; With radiotherapy and usefulness, be 33% to the complete remission rate of the pulmonary carcinoma and the esophageal carcinoma, the notable synergistic effect is arranged.

The object of the present invention is achieved like this: earthworm capsule, get raw material Pheretima alive, wash, add water homogenate, freezing 48 hours, melt filtration, lyophilization incapsulates promptly, its detailed process is: new fresh and alive Pheretima, and tap water is cleaned, airing, 3 times of adding distil waters (W/W), with twice of high-speed tissue mashing machine's (800-12000 rev/min) homogenate, each 2 minutes, 20 seconds at interval, homogenate was put in the cryogenic refrigerator (below 20 ℃) freezing 48 hours, through freeze thawing (＜36 ℃), centrifugal 3000 rev/mins) 20 minutes, filter by (the quick filter paper of two-layer Xinhua), the cool lyophilizing of filtrate dry (pre-cooling-40 ℃, vacuum 10 ², 33 ℃ of sublimation temperatures, moisture content is lower than 70%) the one-tenth brown powder.

Accompanying drawing is preparation technology's flow chart of the present invention.

Advantage of the present invention is: have promoting blood circulation and removing blood stasis, clearing heat and detoxicating, dispersing swelling and dissipating binds, raising is thin Born of the same parents' immunologic function, inhibition tumor cell generates. Be used for the treatment of especially tumor in digestive tract effect of tumour Significantly, also can be used as the synergist effect of radiotherapy, chemotherapy.

Below in conjunction with embodiment in detail the present invention is described in detail:

Get the dark different lip earthworm 1000g of the back of the body, wash, add water 3000ml, homogenate is twice in high-speed tissue mashing machine, each 2 minutes, be crushed to more than the 10um (diameter) for the first time, be crushed to 3-10um (diameter) for the second time, put then below-20 ℃ and freeze 48hr, room temperature is smashed quick thawing for 30 ℃, and centrifugal 3000 change, and filter and abandon precipitate and suspension larger particles, frozen drying becomes brown powder (pre-cooling-40 ℃, vacuum 10 ², sublimation temperature＜33 ℃, moisture is lower than 7%).Divide encapsulated, cobalt 60 _1,000,000Behind the rd irradiation-sterilize promptly.

Claims

1. Dilong Capsules, which is characterized in that raw material Dilong is taken, washed with water, homogenized with water, frozen for 48 hours, melted and filtered, freeze-dried, and packed into capsules to get final product.

2. Dilong Capsules according to claim 1, is characterized in that its concrete preparation process is: fresh and live earthworm, tap water washes and dries, adds distilled water 3 times (W/W), uses high-speed tissue mashing machine ( 8000-12000 rpm) homogenate twice, each time for 2 minutes, with an interval of 20 seconds, the homogenate solution was frozen in a low-temperature refrigerator (below -20°C) for 48 hours, and after freezing and thawing (<36°C, centrifuge at 3000 rpm ) for 20 minutes, filtered, and the filtrate was freeze-dried (pre-cooled at -40°C, vacuum degree 10 ² , sublimation temperature <33°C, water content less than 7%) to brown powder, and then packed into capsules.