CN116998552A - Infant milk powder rich in osteopontin and α-lactalbumin and preparation method thereof - Google Patents

Abstract

The invention discloses an infant milk powder rich in osteopontin and α-lactalbumin and a preparation method thereof. The milk powder includes the following components in weight percentage: concentrated whey protein powder 3 to 8%, skimmed milk 11% ~23%, the mass ratio of the skimmed milk to the concentrated whey protein powder is 1: (0.13~0.73); the concentrated whey protein powder includes the following components in terms of weight percentage: enriched osteopontin The concentrated whey protein powder 13-33%, the concentrated whey protein powder enriched with α-lactalbumin 13-48%, and the ordinary concentrated whey protein powder 19-74%. The composition of osteopontin and α-lactalbumin and the amino acid composition pattern of the infant milk powder provided by the present invention are close to those of breast milk, which can improve the nutritional absorption and utilization rate of infants and young children in the milk powder, and the preparation process is easy to control, which is beneficial to Product stability.

Description

Infant milk powder rich in osteopontin and α-lactalbumin and preparation method thereof

Technical field

The present invention relates to the technical field of infant milk powder, and in particular to an infant milk powder rich in osteopontin and α-lactalbumin and a preparation method thereof.

Background technique

Breast milk is the most ideal natural food for infants and young children after birth. During the growth and development of infants and young children, breast milk can not only provide sufficient energy and nutrients for infants and young children, but also improve their immune function and promote their growth and metabolism. The basic chemical composition of breast milk is protein, fat, carbohydrates, minerals and vitamins, etc. The protein contained in it is a nutrient that is crucial to the growth and development of infants.

Osteopontin (OPN) is a highly phosphorylated acidic glycoprotein that was first discovered in bone cells and is widely distributed in most tissues and body fluids, such as bones, kidneys, urine, milk, etc., and in breast milk The highest content. OPN in breast milk is also called LPN (Lactopontin). OPN is of great significance to the maturation of the infant's immune system, intestinal development, and cognitive development. The average concentration of OPN in breast milk (138mg/L) is much higher than that in cow's milk (18mg/L). However, cow milk OPN has 61% sequence homology with human milk OPN, and both contain similar integrin binding, proteolytic and PTM sites. Therefore, supplementing OPN in infant formula may help to narrow the gap with breast milk. difference.

Alpha-lactalbumin is also an important protein in breast milk. Mature breast milk has the highest α-lactalbumin content among whey proteins, accounting for 36% of the total whey protein content. Alpha-lactalbumin fortification can promote the absorption of iron and zinc; improve infant sleep and affect neurobehavioral development; improve gastrointestinal symptoms such as flatulence, vomiting, and colic in infants and young children. In addition, the sequence homology of α-lactalbumin in breast milk and cow's milk is 74%, but the α-lactalbumin in cow's milk is lower. Therefore, α-lactalbumin needs to be fortified during the design process of infant milk powder. reaches the level of breast milk.

In addition, different protein combinations will also lead to differences in their amino acid composition and patterns, thus affecting their amino acid absorption and utilization. Research shows that the amino acid pattern of breast milk is more suitable for digestion and absorption by infants and young children. Currently, the infant milk powder on the market has not been explored from the aspect of composite fortification with osteopontin and α-lactalbumin, nor has the change in amino acid composition after composite fortification of osteopontin and α-lactalbumin been studied. By rationally regulating the composite form of osteopontin and α-lactalbumin, developing infant milk powder with an amino acid composition more suitable for infants and young children to absorb is also an urgent problem that needs to be solved.

Contents of the invention

In order to overcome the shortcomings of the prior art, one of the objects of the present invention is to provide an infant milk powder rich in osteopontin and α-lactalbumin, the composition of osteopontin and α-lactalbumin, and the composition of amino acids. The composition pattern is close to that of breast milk, which can improve the nutritional absorption and utilization rate of infants and young children.

The second object of the present invention is to provide a method for preparing infant milk powder rich in osteopontin and α-lactalbumin, which has an easy-to-control process and is beneficial to product stability.

One of the purposes of the present invention is achieved by adopting the following technical solutions:

An infant milk powder rich in osteopontin and α-lactalbumin, including the following components in weight percentage: concentrated whey protein powder 3 to 8%, skimmed milk 11 to 23%, the skimmed milk and The mass ratio of the concentrated whey protein powder is 1: (0.13~0.73); the concentrated whey protein powder includes the following components in weight percentage: osteopontin-enriched concentrated whey protein powder 13~33 %, α-lactalbumin-rich concentrated whey protein powder 13-48%, ordinary concentrated whey protein powder 19-74%.

The present invention rationally designs the ratio of concentrated whey protein powder and skim milk, strictly limits the composition of concentrated whey protein powder, and cleverly designs concentrated whey protein powder enriched in osteopontin and concentrated milk enriched in α-lactalbumin. The ratio of albumin protein powder and ordinary concentrated whey protein powder makes the composition of osteopontin and α-lactalbumin of the obtained milk powder close to that of breast milk, and the amino acid composition is close to that of breast milk, which is conducive to the nutritional absorption of milk powder by infants and young children. .

As a preferred embodiment of the present invention, in the osteopontin-enriched concentrated whey protein powder, the proportion of osteopontin is 5% to 7%, and the proportion of α-lactalbumin is 5% to 15%. .

As a preferred embodiment of the present invention, in the α-lactalbumin-enriched concentrated whey protein powder, osteopontin accounts for 0.02% to 0.15%, and α-lactalbumin accounts for 40% to 60%. %.

As a preferred embodiment of the present invention, in the ordinary concentrated whey protein powder, the proportion of osteopontin is 0.2% to 0.7%, and the proportion of α-lactalbumin is 5% to 15%. The above three concentrated whey protein powders can be purchased through commercial channels.

As a preferred embodiment of the present invention, the infant milk powder rich in osteopontin and α-lactalbumin also includes one or any combination of the following components: compound oil, lactose, galacto-oligosaccharide, Docosahexaenoic acid oil, arachidonic acid oil, multivitamin fortifier, multimineral fortifier, taurine, inositol, and other nutrients.

As a preferred embodiment of the present invention, the infant milk powder rich in osteopontin and α-lactalbumin includes the following components in weight percentage: concentrated whey protein powder 3 to 8%, skimmed milk 11 to 8%. 23%, the mass ratio of the skimmed milk to the concentrated whey protein powder is 1: (0.13~0.73); the concentrated whey protein powder includes the following components in weight percentage: osteopontin-enriched Concentrated whey protein powder 13-33%, α-lactalbumin-enriched concentrated whey protein powder 13-48%, ordinary concentrated whey protein powder 19-74%; also includes the following components in weight percentage: Compound oil 21~26%, lactose 41~44%, galactooligosaccharide 7~8%, docosahexaenoic acid oil 0.7~0.9%, arachidonic acid oil 0.2~0.4%, multivitamin fortifier 0.8～1.0%, complex mineral fortifier 1.1～1.5%, taurine 0.02～0.04%, inositol 0.02～0.04%, other nutrients 0.2～1.24%.

As a preferred embodiment of the present invention, the compound oil includes the following components in weight percentage: 1,3-dioleic acid 2-palmitic acid triglyceride 44% to 52%, canola oil 22% to 52%. 29%, anhydrous cream 18~21%, sunflower oil 5~17%, phospholipid 0.45~1.55%, dl-α-tocopherol 0.03~0.04%, ascorbyl palmitate 0.01~0.02%.

As a preferred embodiment of the present invention, the multivitamin fortifier includes the following components in weight percentage: choline bitartrate 48-50%, sodium L-ascorbate 28-29%, folic acid 1.8-2.0%, mixed fertility Phenol concentrate 1.3~1.4%, phytomenadione 0.9~1.0%, vitamin _D3 0.7~0.8%, retinyl acetate 0.6~0.8%, dl-α-tocopheryl acetate 0.6~0.8%, nicotinamide 0.5~ 0.6%, D-calcium pantothenate 0.4~0.5%, cyanocobalamin 0.3~0.4%, D-biotin 0.25~0.30%, thiamine hydrochloride 0.13~0.14%, ascorbyl palmitate 0.1~0.2%, pyridoxine hydrochloride Alcohol 0.07~0.10%, riboflavin 0.01~0.02%, lactose 13.8~14.4%.

As a preferred embodiment of the present invention, the composite mineral enhancer includes the following components in weight percentage: sodium chloride 11-22%, calcium carbonate 1-29%, magnesium chloride 11-13%, potassium chloride 4 ~26%, iron pyrophosphate 4~8%, zinc sulfate 0.8~1.2%, potassium iodide 0.24~1.24%, copper sulfate 0.08~0.12%, manganese sulfate 0.01~0.05%, other minerals 24.87~40.08%.

As a preferred embodiment of the present invention, the other nutritional components at least include the following components: β-carotene and nucleotides, and the nucleotides include one or any combination of the following: cytidine 5' monophosphate, Adenosine 5'monophosphate, disodium 5'-inosinate, disodium 5'-guanylate, disodium 5'-uridylate.

The second object of the present invention is achieved by adopting the following technical solutions:

A method for preparing infant milk powder rich in osteopontin and α-lactalbumin. The infant milk powder includes the following components in weight percentage: concentrated whey protein powder 3 to 8%, skimmed milk 11 to 8%. 23%, compound oil 21~26%, lactose 41~44%, galactooligosaccharide 7~8%, docosahexaenoic acid oil 0.7~0.9%, arachidonic acid oil 0.2~0.4%, compound Vitamin fortifier 0.8~1.0%, complex mineral fortifier 1.1~1.5%, taurine 0.02~0.04%, inositol 0.02~0.04%, other nutrients 0.2~1.24%, including the following steps:

(1) Pre-sterilization: Pre-sterilize skim milk at a sterilization temperature of 105-115°C and a sterilization time of 20-40 seconds;

(2) Ingredients: Weigh skim milk, concentrated whey protein powder, compound oil, lactose, galacto-oligosaccharide, multi-vitamin fortifier, multi-mineral fortifier, docosahexaenoic acid oil and fat in predetermined proportions. Arachidonic acid oil, taurine, inositol and other nutrients are stirred and mixed evenly at 50-60°C to obtain a mixture;

(3) Sterilization: Sterilize the mixture again at a sterilization temperature of 75 to 85°C and a sterilization time of 10 to 20 seconds;

(4) Homogenization: Homogenize the mixture in a high-pressure homogenizer with a homogenization pressure of 160 to 180 bar;

(5) Concentration: further concentrate the mixture, set the first effect body temperature of the concentrator to 50~55℃, the second effect body temperature to 45~55℃, the steam pressure to 3~5bar, and the vacuum degree to - 950~-850mbar;

(6) Spray drying and cooling: Use spray drying equipment for drying, the inlet air temperature is 175~180°C, and the exhaust air temperature is 85~95°C; further increase the secondary drying of the fluidized bed, the inlet air temperature is 25~35°C, The air outlet temperature is 40~50℃, and the powder outlet temperature is 25~30℃;

(7) Product packaging: The product is further monitored through screens and magnets, and then packaged with nitrogen.

Compared with the existing technology, the beneficial effects of the present invention are:

(1) The infant milk powder rich in osteopontin and α-lactalbumin provided by the present invention optimizes the composition of osteopontin and α-lactalbumin, rationally designs and regulates its amino acid composition, and constructs a composition similar to breast milk. The matching amino acid pattern can improve the nutritional absorption and utilization rate of infants and young children in milk powder.

(2) The preparation method of infant milk powder rich in osteopontin and α-lactalbumin provided by the present invention has an easy-to-control process and is beneficial to product stability.

Detailed ways

Below, the present invention will be further described with reference to specific implementation modes. It should be noted that, on the premise of no conflict, the embodiments or technical features described below can be arbitrarily combined to form new embodiments. The raw materials, equipment, etc. used in the following embodiments can be purchased through commercial channels unless otherwise specified.

Example 1

An infant milk powder rich in osteopontin and α-lactalbumin, consisting of the following components in terms of weight percentage: concentrated whey protein powder 8% (of which the concentrated whey protein powder enriched in osteopontin accounts for 13%, α-lactalbumin-rich concentrated whey protein powder accounts for 13%, ordinary concentrated whey protein powder accounts for 74%), skimmed milk (on a dry basis) 11%, which is the same as The ratio of total added amount of concentrated whey protein is 1:0.73; 26% compound oil, 44% lactose, 7% galacto-oligosaccharide, 0.9% docosahexaenoic acid powder, 0.4% arachidonic acid oil. Multivitamin fortifier 1.0%, complex mineral fortifier 1.4%, taurine 0.03%, inositol 0.04%, other nutrients 0.23%.

Among them, the proportion of osteopontin in the concentrated whey protein powder enriched with osteopontin is 5%, and the proportion of α-lactalbumin is 5%; the concentrated whey protein powder enriched with α-lactalbumin is The proportion of osteopontin is 0.02% and the proportion of α-lactalbumin is 40%; the proportion of osteopontin in ordinary concentrated whey protein powder is 0.2% and the proportion of α-lactalbumin is 5%. .

In terms of weight percentage, the compound oil is composed of the following components: 1,3-dioleic acid 2-palmitic acid triglyceride 42%, canola oil 22%, anhydrous cream 18%, sunflower seed oil 16.5% %, phospholipid 1.44%, dl-α-tocopherol 0.04%, ascorbyl palmitate 0.02%.

In terms of weight percentage, the multivitamin fortifier consists of the following components: choline bitartrate 50%, sodium L-ascorbate 28%, folic acid 1.8%, mixed tocopherol concentrate 1.3%, phytomenadione 1.0%, vitamin D ₃ 0.7%, retinyl acetate 0.6%, dl-α-tocopheryl acetate 0.6%, nicotinamide 0.5%, D-calcium pantothenate 0.5%, cyanocobalamin 0.38%, D-biotin 0.25%, thiamine hydrochloride 0.13%, ascorbyl palmitate 0.1%, pyridoxine hydrochloride 0.07%, riboflavin 0.02%, other ingredients (lactose) 14.05%.

In terms of weight percentage, the complex mineral fortifier consists of the following components: sodium chloride 22%, calcium carbonate 20%, magnesium chloride 13%, potassium chloride 4%, iron pyrophosphate 4%, zinc sulfate 1%, potassium iodide 1.2 %, copper sulfate 0.11%, manganese sulfate 0.01%, other minerals 34.68%.

Other nutrients include beta-carotene and nucleotides (cytidine 5' monophosphate, adenosine 5' monophosphate, disodium 5'-inosinate, disodium 5'-guanylate, and 5'-uridine disodium acid).

The preparation method of infant milk powder rich in osteopontin and α-lactalbumin of Example 1 includes the following steps:

(1) Pre-sterilization: Pre-sterilize skim milk at a sterilization temperature of 110°C and a sterilization time of 30 seconds;

(2) Ingredients: Weigh skim milk, concentrated whey protein powder, compound oil, lactose, galacto-oligosaccharide, multivitamin fortifier, multimineral fortifier and other raw materials according to predetermined proportions, and stir at 55°C well mixed;

(3) Sterilization: Sterilize the mixture again at a sterilization temperature of 80°C and a sterilization time of 15 seconds;

(4) Homogenization: Homogenize the mixture in a high-pressure homogenizer with a homogenization pressure of 170bar;

(5) Concentration: The mixture is further concentrated. The temperature of the first effect body is 52°C, the temperature of the second effect body is 49°C, the steam pressure is 4bar, and the vacuum degree is -900mbar;

(6) Spray drying and cooling: Use spray drying equipment for drying, the inlet air temperature is 178°C, and the exhaust air temperature is 90°C for drying; further increase the fluidized bed secondary drying, the inlet air temperature is 30°C, and the outlet air temperature is 44°C. , the powder output temperature is 27℃;

(7) Product packaging: The product is further monitored through screens and magnets, and then packaged with nitrogen.

Example 2

An infant milk powder rich in osteopontin and α-lactalbumin, consisting of the following components in terms of weight percentage: concentrated whey protein powder 4.8% (of which the concentrated whey protein powder enriched in osteopontin accounts for 21%, α-lactalbumin-rich concentrated whey protein powder accounts for 31%, ordinary concentrated whey protein powder accounts for 48%), skimmed milk (on a dry basis) 18.5%, which is the same as The ratio of the total amount of whey protein concentrate added is 1:0.26. Compound oil 25%, lactose 41%, galactooligosaccharide 7.2%, docosahexaenoic acid powder 0.85%, arachidonic acid oil 0.39%, multivitamin fortifier 0.9%, complex mineral fortifier 1.1% , taurine 0.04%, inositol 0.02%, other nutrients 0.2%.

Among them, the proportion of osteopontin in the concentrated whey protein powder enriched with osteopontin is 6%, and the proportion of α-lactalbumin is 10%; in the concentrated whey protein powder enriched with α-lactalbumin, The proportion of osteopontin is 0.10% and the proportion of α-lactalbumin is 50%; the proportion of osteopontin in ordinary concentrated whey protein powder is 0.5% and the proportion of α-lactalbumin is 10%. .

In terms of weight percentage, the compound oil is composed of the following components: 1,3-dioleic acid 2-palmitic acid triglyceride 44.45%, canola oil 28.5%, anhydrous cream 20%, sunflower oil 6.5% %, phospholipid 0.5%, dl-α-tocopherol 0.04%, ascorbyl palmitate 0.01%.

The multivitamin fortifier is composed of the following components in percentage by weight: the following components are mixed in percentage by weight: choline bitartrate 49%, sodium L-ascorbate 28.7%, folic acid 1.9% , mixed tocopherol concentrate 1.38%, phytomenadione 0.9%, vitamin D ₃ 0.8%, retinyl acetate 0.8%, dl-alpha-tocopheryl acetate 0.7%, nicotinamide 0.5%, D-calcium pantothenate 0.5% , cyanocobalamin 0.3%, D-biotin 0.28%, thiamine hydrochloride 0.14%, ascorbyl palmitate 0.2%, pyridoxine hydrochloride 0.08%, riboflavin 0.01%, other ingredients (lactose) is 13.81%.

In terms of weight percentage, the complex mineral fortifier consists of the following components: sodium chloride 11%, calcium carbonate 2%, magnesium chloride 12%, potassium chloride 26.31%, iron pyrophosphate 8%, zinc sulfate 1.2%, potassium iodide 1.24 %, copper sulfate 0.12%, manganese sulfate 0.05%, other minerals 40.08%.

Other nutrients include beta-carotene and nucleotides (cytidine 5' monophosphate, adenosine 5' monophosphate, disodium 5'-inosinate, disodium 5'-guanylate, and 5'-uridine disodium acid).

The preparation method of infant milk powder rich in osteopontin and α-lactalbumin of Example 2 includes the following steps:

(1) Pre-sterilization: Pre-sterilize skim milk at a sterilization temperature of 110°C and a sterilization time of 30 seconds;

(2) Ingredients: Weigh skim milk, concentrated whey protein powder, compound oil, lactose, galacto-oligosaccharide, multi-vitamin fortifier, multi-mineral fortifier and other raw materials according to predetermined proportions, and stir and mix at 55°C uniform; even

(3) Sterilization: Sterilize the mixture again at a sterilization temperature of 80°C and a sterilization time of 15 seconds;

(4) Homogenization: Homogenize the mixture in a high-pressure homogenizer with a homogenization pressure of 170bar;

(5) Concentration: The mixture is further concentrated. The temperature of the first effect body is 52°C, the temperature of the second effect body is 49°C, the steam pressure is 4bar, and the vacuum degree is -900mbar;

(6) Spray drying and cooling: Use spray drying equipment for drying, the inlet air temperature is 178°C, and the exhaust air temperature is 90°C for drying; further increase the fluidized bed secondary drying, the inlet air temperature is 30°C, and the outlet air temperature is 44°C. , the powder output temperature is 27℃;

(7) Product packaging: The product is further monitored through screens and magnets, and then packaged with nitrogen.

Example 3

An infant formula milk powder rich in osteopontin and alpha-lactalbumin, consisting of the following components in weight percentage: concentrated whey protein powder 3% (of which the concentrated whey protein powder enriched in osteopontin accounts for The proportion of concentrated whey protein powder enriched with α-lactalbumin is 48%, the proportion of ordinary concentrated whey protein powder is 19%), and the skimmed milk (on a dry basis) is 23%. The ratio to the total amount of whey protein concentrate is 1:0.13. Compound oil 21%, lactose 41%, galactooligosaccharide 7.5%, docosahexaenoic acid powder 0.7%, arachidonic acid oil 0.2%, multivitamin fortifier 0.8%, complex mineral fortifier 1.5% , taurine 0.04%, inositol 0.02%, other nutrients 1.24%.

Among them, the proportion of osteopontin in the concentrated whey protein powder enriched with osteopontin is 7%, and the proportion of α-lactalbumin is 15%; in the concentrated whey protein powder enriched with α-lactalbumin, The proportion of osteopontin is 0.15% and the proportion of α-lactalbumin is 60%; the proportion of osteopontin in ordinary concentrated whey protein powder is 0.7% and the proportion of α-lactalbumin is 15%. .

In terms of weight percentage, the compound oil is composed of the following components: 1,3-dioleic acid 2-palmitic acid triglyceride 51.5%, canola oil 22.5%, anhydrous cream 20.5%, sunflower oil 5.0 %, phospholipid 0.45%, dl-α-tocopherol 0.03%, ascorbyl palmitate 0.02%.

In terms of weight percentage, the multivitamin fortifier consists of the following components: choline bitartrate 48%, sodium L-ascorbate 29%, folic acid 2.0%, mixed tocopherol concentrate 1.4%, phytomenadione 1.0%, vitamin D ₃ 0.8%, retinyl acetate 0.7%, dl-α-tocopherol acetate 0.8%, nicotinamide 0.6%, D-calcium pantothenate 0.4%, cyanocobalamin 0.4%, D-biotin 0.3%, thiamine hydrochloride 0.14%, ascorbyl palmitate 0.1%, pyridoxine hydrochloride 0.1%, riboflavin 0.01%, and other ingredients (lactose) 14.25%.

In terms of weight percentage, the complex mineral fortifier consists of the following components: sodium chloride 15%, calcium carbonate 29%, magnesium chloride 11%, potassium chloride 13%, iron pyrophosphate 6%, zinc sulfate 0.8%, potassium iodide 0.24 %, copper sulfate 0.08%, manganese sulfate 0.01%, other minerals 24.87%.

Other nutrients include beta-carotene and nucleotides (cytidine 5' monophosphate, adenosine 5' monophosphate, disodium 5'-inosinate, disodium 5'-guanylate, and 5'-uridine disodium acid).

The preparation method of infant formula milk powder rich in osteopontin and α-lactalbumin of Example 3 includes the following steps:

(1) Pre-sterilization: Pre-sterilize skim milk at a sterilization temperature of 110°C and a sterilization time of 30 seconds;

(2) Ingredients: Weigh skim milk, concentrated whey protein powder, compound oil, lactose, galacto-oligosaccharide, multi-vitamin fortifier, multi-mineral fortifier and other raw materials according to predetermined proportions, and stir and mix at 55°C uniform; even

(3) Sterilization: Sterilize the mixture again at a sterilization temperature of 80°C and a sterilization time of 15 seconds;

(4) Homogenization: Homogenize the mixture in a high-pressure homogenizer with a homogenization pressure of 170bar;

(5) Concentration: The mixture is further concentrated. The temperature of the first effect body is 52°C, the temperature of the second effect body is 49°C, the steam pressure is 4bar, and the vacuum degree is -900mbar;

(6) Spray drying and cooling: Use spray drying equipment for drying, the inlet air temperature is 178°C, and the exhaust air temperature is 90°C for drying; further increase the fluidized bed secondary drying, the inlet air temperature is 30°C, and the outlet air temperature is 44°C. , the powder output temperature is 27℃;

(7) Product packaging: The product is further monitored through screens and magnets, and then packaged with nitrogen.

Comparative example 1

An infant milk powder is composed of the following components in terms of weight percentage: 9% concentrated whey protein powder, which is only ordinary concentrated whey protein powder (the proportion of osteopontin is 0.5%, α -The proportion of whey protein is 10%), skimmed milk (on a dry basis) 10%, the ratio to the total amount of whey protein concentrate is 1:0.9; mixed fats and oils are 24%, lactose is 53%, low Polygalactose 0.9%, docosahexaenoic acid powder 0.1%, arachidonic acid oil 0.1%, multivitamin fortifier 0.35%, complex mineral fortifier 0.7%, other nutrients 1.85%.

In the milk powder of Comparative Example 1, the dosage range of concentrated whey protein powder and skimmed milk exceeds the scope of the present invention, and the concentrated whey protein powder only uses ordinary concentrated whey protein powder, excluding osteopontin-enriched concentrated milk. Albumin protein powder and whey protein concentrate powder enriched with alpha-lactalbumin.

Comparative example 2

An infant milk powder consists of the following components in terms of weight percentage: 9% concentrated whey protein powder (including 88.76% ordinary concentrated whey protein powder and 11.24% concentrated whey protein powder enriched with α-lactalbumin) ; Skim milk (on a dry basis) 12%, the ratio to the total amount of whey protein concentrate is 1:0.75, mixed oil 24%, lactose 47%, galactooligosaccharide 5%, docosahexane Acrylic acid powder 0.5%, arachidonic acid oil 0.2%, multivitamin fortifier 0.3%, complex mineral fortifier 0.8%, other nutrients 1.2%.

Among them, the proportion of osteopontin in ordinary concentrated whey protein powder is 0.5%, and the proportion of α-lactalbumin is 10%; the proportion of osteopontin in concentrated whey protein powder enriched with α-lactalbumin is The ratio is 0.10%, and the α-lactalbumin ratio is 50%.

In the milk powder of Comparative Example 2, the dosage range of concentrated whey protein powder and skimmed milk exceeds the scope of the present invention, and the concentrated whey protein powder only uses ordinary concentrated whey protein powder and concentrated milk enriched with α-lactalbumin. Albumin protein powder, excluding osteopontin-enriched whey protein concentrate powder.

Comparative example 3

An infant milk powder is composed of the following components in terms of weight percentage: 9% concentrated whey protein powder (including 12% concentrated whey protein powder enriched with osteopontin, 11% concentrated whey protein enriched with α-lactalbumin) Whey protein powder, 77% ordinary concentrated whey protein powder); skimmed milk (on a dry basis) 10%, the ratio to the total amount of concentrated whey protein added is 1:0.9, mixed oil 25%, lactose 50 %, galactooligosaccharide 1%, docosahexaenoic acid powder 0.9%, arachidonic acid oil 0.4%, multivitamin fortifier 0.35%, complex mineral fortifier 0.8%, other nutrients 2.55%.

Among them, the proportion of osteopontin in the concentrated whey protein powder enriched with osteopontin is 6%, and the proportion of α-lactalbumin is 10%; in the concentrated whey protein powder enriched with α-lactalbumin, The proportion of osteopontin is 0.10% and the proportion of α-lactalbumin is 50%; the proportion of osteopontin in ordinary concentrated whey protein powder is 0.5% and the proportion of α-lactalbumin is 10%. .

In the milk powder of Comparative Example 3, the dosage range of the concentrated whey protein powder and skimmed milk is beyond the scope of the present invention, and the ratio of the three concentrated whey protein powders is also beyond the scope of the present invention.

Effect verification

The milk powder and breast milk obtained in Examples 1-3 and Comparative Examples 1-3 of the present invention were taken, and the osteopontin and α-lactalbumin contents were measured. The results are shown in Table 1.

Table 1 Comparison of osteopontin and α-lactalbumin content

Note: The osteopontin concentration data in breast milk comes from "Zhu Jing, Research Progress on the Active Component of Human Milk-Osteopontin [J], Journal of Clinical Pediatrics. 2021.39(4)." The data is the average concentration of osteopontin in human milk. .

The α-lactalbumin concentration data in breast milk comes from "Bian Xiaoming, Nutritional Composition and Function of Breast Milk and Formula Milk [J]. Chinese Pediatric Emergency Medicine. 2007.14(1).

As can be seen from Table 1, compared with Comparative Examples 1-3, the concentrations of osteopontin and α-lactalbumin in Examples 1-3 are closer to the average concentrations in breast milk. Moreover, the concentration ratio of osteopontin to α-lactalbumin in Examples 1-3 is within the ratio range of breast milk, while the ratio of Comparative Examples 1-3 is beyond the ratio range of breast milk.

The amino acid compositions of Examples 1-3, Comparative Examples 1-3 and breast milk were further tested and compared, and the results are shown in Table 2.

Table 2 Comparison of amino acid composition

Note: Among them, the amino acid composition data of breast milk comes from the research data of 1640 breast milk samples from 8 provinces and cities in China (Beijing, Jinan, Baotou, Chengdu, Kunming, Shenzhen, Nanjing, Xining) (Ren Lele, Construction of Breast Milk Amino Acid Pattern, 2022 .).

It can be seen from the results in Table 2 that compared with the comparative examples, the amino acid composition of the infant milk powder rich in osteopontin and α-lactalbumin prepared in Examples 1-3 of the present invention is closer to that of breast milk, and the amino acid composition contained therein is Essential amino acids for infants such as phenylalanine, valine, isoleucine, leucine, lysine, threonine, and tryptophan are all within the fluctuation range of breast milk amino acids.

The present invention rationally prepares the proportions of concentrated whey protein and skimmed milk, and at the same time cleverly designs the proportions of three concentrated whey proteins, thereby adjusting the proportions and amino acid compositions of osteopontin and α-lactalbumin in the formula. Regulation to achieve an amino acid pattern that matches breast milk, promote infants and young children’s digestion and absorption of milk powder, and improve the absorption and utilization of amino acids.

The above-mentioned embodiments are only preferred embodiments of the present invention and cannot be used to limit the scope of protection of the present invention. Any non-substantive changes and substitutions made by those skilled in the art on the basis of the present invention fall within the scope of the present invention. Scope of protection claimed.

Claims (10)

1. Infant milk powder enriched in osteopontin and alpha-lactalbumin, characterized by comprising the following components in percentage by weight: 3-8% of concentrated whey protein powder and 11-23% of skimmed cow milk, wherein the mass ratio of the skimmed cow milk to the concentrated whey protein powder is 1: (0.13-0.73); the concentrated whey protein powder comprises the following components in percentage by weight: concentrated whey protein powder enriched with osteopontin 13-33%, concentrated whey protein powder enriched with alpha-lactalbumin 13-48% and common concentrated whey protein powder 19-74%.

2. The infant milk powder enriched in osteopontin and alpha-lactalbumin as claimed in claim 1, wherein the concentration of the whey protein powder enriched in osteopontin is 5% -7% and the concentration of the alpha-lactalbumin is 5% -15%;

in the concentrated whey protein powder enriched with the alpha-lactalbumin, the ratio of the osteopontin is 0.02-0.15 percent, and the ratio of the alpha-lactalbumin is 40-60 percent;

in the common concentrated whey protein powder, the ratio of the osteopontin is 0.2-0.7%, and the ratio of the alpha-whey protein is 5-15%.

3. The infant milk powder enriched in osteopontin and alpha-lactalbumin as claimed in claim 1, further comprising one or any combination of the following components: the compound oil, lactose, galacto-oligosaccharide, docosahexaenoic acid oil, arachidonic acid oil, compound vitamin enhancer, compound mineral enhancer, taurine, inositol and other nutritional ingredients.

4. Infant milk powder enriched in osteopontin and alpha-lactalbumin according to claim 1, further comprising the following components in weight percent: 21-26% of compound grease, 41-44% of lactose, 7-8% of galacto-oligosaccharide, 0.7-0.9% of docosahexaenoic acid grease, 0.2-0.4% of arachidonic acid grease, 0.8-1.0% of compound vitamin enhancer, 1.1-1.5% of compound mineral enhancer, 0.02-0.04% of taurine, 0.02-0.04% of inositol and 0.2-1.24% of other nutritional ingredients.

5. Infant milk powder enriched in osteopontin and alpha-lactalbumin as claimed in claim 4, wherein the compound grease comprises the following components in weight percent: 44-52% of 1, 3-dioleate 2-palmitic acid triglyceride, 22-29% of canola oil, 18-21% of anhydrous cream, 5-17% of sunflower seed oil, 0.45-1.55% of phospholipid, 0.03-0.04% of dl-alpha-tocopherol and 0.01-0.02% of ascorbyl palmitate.

6. Infant milk powder enriched in osteopontin and alpha-lactalbumin as claimed in claim 4, wherein the multivitamin fortifier comprises the following components in weight percent: 48 to 50 percent of choline bitartrate, 28 to 29 percent of L-sodium ascorbate, 1.8 to 2.0 percent of folic acid, 1.3 to 1.4 percent of mixed tocopherol concentrate, 0.9 to 1.0 percent of phytomenadione and vitamin D ₃ 0.7 to 0.8 percent, 0.6 to 0.8 percent of retinyl acetate, 0.6 to 0.8 percent of dl-alpha-tocopheryl acetate, 0.5 to 0.6 percent of nicotinamide, 0.4 to 0.5 percent of D-calcium pantothenate, 0.3 to 0.4 percent of cyanocobalamine, 0.25 to 0.30 percent of D-biotin, 0.13 to 0.14 percent of thiamine hydrochloride, 0.1 to 0.2 percent of ascorbyl palmitate, 0.07 to 0.10 percent of pyridoxine hydrochloride, 0.01 to 0.02 percent of riboflavin and 13.8 to 14.4 percent of lactose.

7. Infant milk powder enriched in osteopontin and alpha-lactalbumin as claimed in claim 4, wherein the complex mineral fortifier comprises the following components in weight percent: 11 to 22 percent of sodium chloride, 1 to 29 percent of calcium carbonate, 11 to 13 percent of magnesium chloride, 4 to 27 percent of potassium chloride, 4 to 8 percent of ferric pyrophosphate, 0.8 to 1.2 percent of zinc sulfate, 0.24 to 1.24 percent of potassium iodide, 0.08 to 0.12 percent of copper sulfate, 0.01 to 0.05 percent of manganese sulfate and 24.87 to 40.08 percent of other minerals.

8. Infant milk powder enriched in osteopontin and alpha-lactalbumin as claimed in claim 4, wherein the other nutritional ingredients comprise the following components: beta-carotene and nucleotides.

9. Infant milk powder enriched in osteopontin and alpha-lactalbumin according to claim 8, wherein the nucleotides comprise one or any combination of the following: cytidine 5' -monophosphate, adenosine 5' -monophosphate, disodium 5' -inosinate, disodium 5' -guanylate, disodium 5' -uridylate.

10. The preparation method of the infant milk powder rich in osteopontin and alpha-lactalbumin is characterized in that the infant milk powder comprises the following components in percentage by weight: 3 to 8 percent of concentrated whey protein powder, 11 to 23 percent of skim milk, 21 to 26 percent of compound grease, 41 to 44 percent of lactose, 7 to 8 percent of galacto-oligosaccharide, 0.7 to 0.9 percent of docosahexaenoic acid grease, 0.2 to 0.4 percent of arachidonic acid grease, 0.8 to 1.0 percent of compound vitamin enhancer, 1.1 to 1.5 percent of compound mineral enhancer, 0.02 to 0.04 percent of taurine, 0.02 to 0.04 percent of inositol and 0.2 to 1.24 percent of other nutritional ingredients, comprising the following steps:

(1) Pre-sterilization: pre-sterilizing the defatted milk at 105-115 deg.c for 20-40 s;

(2) And (3) batching: weighing skim milk, concentrated whey protein powder, compound grease, lactose, galactooligosaccharide, compound vitamin enhancer, compound mineral enhancer, docosahexaenoic acid grease, arachidonic acid grease, taurine, inositol and other nutritional ingredients according to a preset proportion, and stirring and uniformly mixing at 50-60 ℃ to obtain a mixture;

(3) Sterilizing: re-sterilizing the mixture at the sterilization temperature of 75-85 ℃ for 10-20 s;

(4) Homogenizing: homogenizing the mixture in a high-pressure homogenizer at a homogenizing pressure of 160-180 bar;

(5) Concentrating: further concentrating the mixture, setting the temperature of the first effective body of the concentrator to be 50-55 ℃, the temperature of the second effective body of the concentrator to be 45-55 ℃, the steam pressure to be 3-5 bar, and the vacuum degree to be-950 to-850 mbar;

(6) Spray drying and cooling: drying by using spray drying equipment, wherein the air inlet temperature is 175-180 ℃, and the air exhaust temperature is 85-95 ℃; further improves the secondary drying of the fluidized bed, the air inlet temperature is 25-35 ℃, the air outlet temperature is 40-50 ℃, and the powder outlet temperature is 25-30 ℃.