CN116925199B - Antimicrobial peptide SETIT, encoding DNA and expression system - Google Patents
Antimicrobial peptide SETIT, encoding DNA and expression system Download PDFInfo
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- CN116925199B CN116925199B CN202310944047.2A CN202310944047A CN116925199B CN 116925199 B CN116925199 B CN 116925199B CN 202310944047 A CN202310944047 A CN 202310944047A CN 116925199 B CN116925199 B CN 116925199B
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- 239000003910 polypeptide antibiotic agent Substances 0.000 title claims abstract description 37
- 102000044503 Antimicrobial Peptides Human genes 0.000 title abstract description 9
- 108700042778 Antimicrobial Peptides Proteins 0.000 title abstract description 9
- 235000014663 Kluyveromyces fragilis Nutrition 0.000 claims abstract description 8
- 235000018368 Saccharomyces fragilis Nutrition 0.000 claims abstract description 8
- 229940031154 kluyveromyces marxianus Drugs 0.000 claims abstract description 8
- 241000235650 Kluyveromyces marxianus Species 0.000 claims abstract 2
- 125000003275 alpha amino acid group Chemical group 0.000 claims abstract 2
- 230000002401 inhibitory effect Effects 0.000 claims abstract 2
- 239000003242 anti bacterial agent Substances 0.000 claims description 8
- 241000222178 Candida tropicalis Species 0.000 claims description 4
- 241000193388 Bacillus thuringiensis Species 0.000 claims description 3
- 241000588749 Klebsiella oxytoca Species 0.000 claims description 3
- 241000589776 Pseudomonas putida Species 0.000 claims description 3
- 241000191967 Staphylococcus aureus Species 0.000 claims description 3
- 108091028043 Nucleic acid sequence Proteins 0.000 claims description 2
- 230000000844 anti-bacterial effect Effects 0.000 abstract description 12
- 241000894006 Bacteria Species 0.000 abstract description 7
- 241000233866 Fungi Species 0.000 abstract description 5
- 241000044541 Paspalum vaginatum Species 0.000 abstract description 3
- 244000062793 Sorghum vulgare Species 0.000 abstract description 3
- 235000019713 millet Nutrition 0.000 abstract description 3
- 239000000203 mixture Substances 0.000 description 11
- 108020004414 DNA Proteins 0.000 description 9
- 244000253911 Saccharomyces fragilis Species 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 4
- TWRXJAOTZQYOKJ-UHFFFAOYSA-L Magnesium chloride Chemical compound [Mg+2].[Cl-].[Cl-] TWRXJAOTZQYOKJ-UHFFFAOYSA-L 0.000 description 4
- 239000008103 glucose Substances 0.000 description 4
- SCVFZCLFOSHCOH-UHFFFAOYSA-M potassium acetate Chemical compound [K+].CC([O-])=O SCVFZCLFOSHCOH-UHFFFAOYSA-M 0.000 description 4
- 241000192125 Firmicutes Species 0.000 description 3
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 3
- 150000001413 amino acids Chemical group 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 229940079593 drug Drugs 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- -1 salt ion Chemical class 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 239000000126 substance Substances 0.000 description 3
- 206010059866 Drug resistance Diseases 0.000 description 2
- 108091028664 Ribonucleotide Proteins 0.000 description 2
- 229940088710 antibiotic agent Drugs 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 229940041514 candida albicans extract Drugs 0.000 description 2
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000284 extract Substances 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 229910001629 magnesium chloride Inorganic materials 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 229930014626 natural product Natural products 0.000 description 2
- 235000011056 potassium acetate Nutrition 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000002336 ribonucleotide Substances 0.000 description 2
- 125000002652 ribonucleotide group Chemical group 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 238000011144 upstream manufacturing Methods 0.000 description 2
- 239000012138 yeast extract Substances 0.000 description 2
- 108091026890 Coding region Proteins 0.000 description 1
- 241000196324 Embryophyta Species 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- 108010059993 Vancomycin Proteins 0.000 description 1
- 239000003674 animal food additive Substances 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 229940124350 antibacterial drug Drugs 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 229940097012 bacillus thuringiensis Drugs 0.000 description 1
- 238000009412 basement excavation Methods 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000009509 drug development Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 210000003734 kidney Anatomy 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 244000144977 poultry Species 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000006228 supernatant Substances 0.000 description 1
- 229960003165 vancomycin Drugs 0.000 description 1
- MYPYJXKWCTUITO-LYRMYLQWSA-N vancomycin Chemical compound O([C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1=C2C=C3C=C1OC1=CC=C(C=C1Cl)[C@@H](O)[C@H](C(N[C@@H](CC(N)=O)C(=O)N[C@H]3C(=O)N[C@H]1C(=O)N[C@H](C(N[C@@H](C3=CC(O)=CC(O)=C3C=3C(O)=CC=C1C=3)C(O)=O)=O)[C@H](O)C1=CC=C(C(=C1)Cl)O2)=O)NC(=O)[C@@H](CC(C)C)NC)[C@H]1C[C@](C)(N)[C@H](O)[C@H](C)O1 MYPYJXKWCTUITO-LYRMYLQWSA-N 0.000 description 1
- MYPYJXKWCTUITO-UHFFFAOYSA-N vancomycin Natural products O1C(C(=C2)Cl)=CC=C2C(O)C(C(NC(C2=CC(O)=CC(O)=C2C=2C(O)=CC=C3C=2)C(O)=O)=O)NC(=O)C3NC(=O)C2NC(=O)C(CC(N)=O)NC(=O)C(NC(=O)C(CC(C)C)NC)C(O)C(C=C3Cl)=CC=C3OC3=CC2=CC1=C3OC1OC(CO)C(O)C(O)C1OC1CC(C)(N)C(O)C(C)O1 MYPYJXKWCTUITO-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/10—Antimycotics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
- C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
- C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Biotechnology (AREA)
- General Chemical & Material Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Mycology (AREA)
- Oncology (AREA)
- Biochemistry (AREA)
- Communicable Diseases (AREA)
- Biomedical Technology (AREA)
- Biophysics (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Botany (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
本发明公开了一种抗菌肽SETIT，来源于Paspalum vaginatum，其氨基酸序列如SEQ ID NO:1所示。还公开了其编码DNA及表达系统。本发明的抗菌肽SETIT，对革兰氏阳性、阴性菌、真菌均有着明显地抑制效果，抑菌效率高，广谱性好。利用可食用马克斯克鲁维酵母的无细胞表达系统对抗菌肽SETIT进行表达，可以获得可食用级别的谷子抗菌肽。
The present invention discloses an antimicrobial peptide SETIT, which is derived from Paspalum vaginatum and has an amino acid sequence as shown in SEQ ID NO: 1. Also disclosed are its encoding DNA and expression system. The antimicrobial peptide SETIT of the present invention has an obvious inhibitory effect on Gram-positive, Gram-negative bacteria and fungi, has high antibacterial efficiency and good broad-spectrum. By expressing the antimicrobial peptide SETIT using an edible Kluyveromyces marxianus cell-free expression system, an edible millet antimicrobial peptide can be obtained.
Description
Technical Field
The invention relates to an antibacterial peptide SETIT, coding DNA and an expression system, and relates to the technical field of biological products.
Background
Since the advent of antibiotics, antibacterial agents have been applied to our living and health aspects such as the fields of human body infection, pets of poultry and livestock, aquaculture, foods and crops, etc., especially human body infection treatment, but since the long-term high-frequency use, the existing antibiotics have produced a wide range of drug resistance in the human body, in which superbacteria such as vancomycin enterococci have been found to be almost antibiotic-free, and thus, the development of novel antibacterial agents has been unprecedented. At present, most antibacterial drug development mainly focuses on the excavation of novel antibacterial natural products, the chemical structures of the natural products are often complex, large-scale chemical synthesis or biosynthesis is carried out, the antibacterial mechanism of the chemical is single, and the chemical is easy to generate drug resistance when frequently used. In addition, such complex compound drugs often accompany the kidney burden and various side effects, so the development of new antibacterial agents is an important point of current research and development.
An antimicrobial peptide is a protein molecule that has bactericidal and antiviral properties. The antimicrobial peptides can be naturally produced in the body of animals and plants, or can be obtained synthetically. The antibacterial peptide can be used for preparing feed additives, antibacterial agents, medicines, health products and the like, and has wide application prospect. The size of the antimicrobial peptide may vary depending on its source and type.
Disclosure of Invention
The invention discloses an antibacterial peptide SETIT which is derived from Paspalum vaginatum and has an amino acid sequence shown in SEQ ID NO. 1.
Also discloses a coding DNA of the antibacterial peptide SETIT, and the sequence is shown as SEQ ID NO. 2.
An expression system for the antimicrobial peptide SETIT is also disclosed.
Preferably, the expression system is a cell-free expression system of edible kluyveromyces marxianus, and the antibacterial peptide prepared by adopting the cell-free expression system of the kluyveromyces marxianus is the edible antibacterial peptide. Other conventional cellular expression systems may also be used for expression.
Preferably, the expression system comprises a Kluyveromyces marxianus cell extract with the concentration of 30-70% V/V, a 1-3mM ribonucleotide mixture, a 0.05-0.2mM amino acid mixture, a salt ion solution and glucose, and further comprises an expression DNA template of the antibacterial peptide SETIT, wherein the expression DNA template of the antibacterial peptide SETIT is obtained by adding a promoter upstream of the encoding DNA of the antibacterial peptide SETIT.
Preferably, the DNA sequence of the promoter is shown as SEQ ID NO. 3.
Preferably, the salt ion solution comprises 10-100mM potassium acetate, 10-100mM sodium chloride and 0.2-1mM magnesium chloride, and the concentration of glucose is 50mM.
Preferably, the concentration of the expressed DNA template of the antibacterial peptide SETIT is 0.1-1mg/L.
The invention discloses an antibacterial peptide SETIT derived from millet Paspalum vaginatum, which has obvious inhibition effect on gram positive bacteria, gram negative bacteria and fungi, high antibacterial efficiency and good broad spectrum. The antibacterial peptide SETIT is expressed by using a cell-free expression system of edible kluyveromyces marxianus, so that the edible grade millet antibacterial peptide can be obtained.
Drawings
FIG. 1 shows the results of antibacterial activity test of the antibacterial peptide SETIT mixture against Staphylococcus aureus.
FIG. 2 shows the results of antibacterial activity test of the antibacterial peptide SETIT mixture against Bacillus thuringiensis.
FIG. 3 shows the results of antibacterial activity test of the antibacterial peptide SETIT mixture against Pseudomonas putida.
FIG. 4 shows the results of antibacterial activity test of the antibacterial peptide SETIT mixture against Klebsiella oxytoca.
FIG. 5 shows the results of antibacterial activity test of the antibacterial peptide SETIT mixture on Candida tropicalis.
Detailed Description
The invention is further illustrated by the following examples, which are not to be construed as limiting the invention, in conjunction with the accompanying drawings. Specific materials and sources thereof used in embodiments of the present invention are provided below. It will be understood that these are merely exemplary and are not intended to limit the invention, as materials identical or similar to the type, model, quality, nature or function of the reagents and instruments described below may be used in the practice of the invention. The experimental methods used in the following examples are conventional methods unless otherwise specified. Materials, reagents and the like used in the examples described below are commercially available unless otherwise specified.
Example 1
Kluyveromyces marxianus was inoculated into a medium (2% yeast extract, 4% glucose) containing 50ml of YD, and cultured at 30℃and 220rpm for 48 hours. And centrifugally collecting thalli, suspending with normal saline, crushing under high pressure, and centrifugally taking supernatant to prepare the yeast extract. Cell-free Synthesis System 50% Kluyveromyces marxianus cell extract, 2mM ribonucleotide mixture (NTP mix), 0.1mM amino acid mixture, salt ion solution (20 mM potassium acetate, 0.5mM magnesium chloride), 50mM glucose.
And (3) inoculating 10ug of antibacterial peptide SETIT to a 5ml system to express a DNA template, wherein the sequence of the antibacterial peptide SETIT expressing the DNA template is obtained by adding a promoter sequence shown as SEQ ID No.3 to the upstream of a coding sequence shown as SEQ ID No.2, and reacting at 37 ℃ overnight to obtain the edible antibacterial peptide SETIT mixed solution.
Example 2
To test antibacterial activity of the antibacterial peptide SETIT, bacteria were selected from the group consisting of Staphylococcus aureus Staphylococcus aureus (CCTCC: AB 94004), bacillus thuringiensis Bacillus thuringiensis (CCTCC: AB 93100), gram-negative bacteria, pseudomonas putida Pseudomonas putida (CCTCC: PP), klebsiella oxytoca Klebsiella oxytoca (CCTCC: AB 2010143), and fungi (Candida tropicalis) (Candida tropicalis). The above gram positive bacteria, gram negative bacteria and fungi were all purchased from the China center for type culture Collection.
The gram positive bacteria, gram negative bacteria, fungi and multi-drug resistant bacteria are cultured in a 96-well plate containing 100uL response culture medium, OD600 is cultured to 0.2, 0, 0.01%, 0.1%, 1% and 10% of the antibacterial peptide SETIT mixed solution obtained in the V/V example 1 and 10ug/L Nisin are respectively added, the mixture is cultured for 12 hours at 37 ℃, an enzyme-labeled instrument is used for detecting the OD600 value of a sample in the 96-well plate, and the result is shown in figures 1-5, so that the antibacterial peptide SETIT fermentation broth produced by the invention has good antibacterial capacity.
Claims (3)
1. The application of the antibacterial peptide SETIT in preparation of an antibacterial agent is characterized in that the amino acid sequence of the antibacterial peptide SETIT is shown as SEQ ID NO. 1, and the antibacterial agent is used for inhibiting staphylococcus aureus Staphylococcus aureus, bacillus thuringiensis Bacillus thuringiensis, pseudomonas putida Pseudomonas putida, klebsiella oxytoca Klebsiella oxytoca or candida tropicalis Candida tropicalis.
2. The use according to claim 1, characterized in that the DNA sequence encoding the antibacterial peptide SETIT is shown in SEQ ID NO. 2.
3. Use according to claim 1, characterized in that the expression system of the antibacterial peptide SETIT is a cell-free expression system of kluyveromyces marxianus.
Priority Applications (1)
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| CN202310944047.2A CN116925199B (en) | 2023-07-31 | 2023-07-31 | Antimicrobial peptide SETIT, encoding DNA and expression system |
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| CN202310944047.2A CN116925199B (en) | 2023-07-31 | 2023-07-31 | Antimicrobial peptide SETIT, encoding DNA and expression system |
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| CN116925199A CN116925199A (en) | 2023-10-24 |
| CN116925199B true CN116925199B (en) | 2025-04-18 |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110195059A (en) * | 2019-05-29 | 2019-09-03 | 中国科学院水生生物研究所 | Nucleic acid molecules, expression vector and application, antibacterials and method |
| CN114317305A (en) * | 2021-12-31 | 2022-04-12 | 康码（上海）生物科技有限公司 | A kind of eukaryotic cell and method that can increase the expression level of cell-free protein |
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| CA3097742A1 (en) * | 2018-04-18 | 2019-10-24 | Ligandal, Inc. | Methods and compositions for genome editing |
| CN111202839A (en) * | 2020-02-21 | 2020-05-29 | 佛山科学技术学院 | Application of a kind of histone H4 in inhibiting avian influenza virus |
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Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110195059A (en) * | 2019-05-29 | 2019-09-03 | 中国科学院水生生物研究所 | Nucleic acid molecules, expression vector and application, antibacterials and method |
| CN114317305A (en) * | 2021-12-31 | 2022-04-12 | 康码（上海）生物科技有限公司 | A kind of eukaryotic cell and method that can increase the expression level of cell-free protein |
Non-Patent Citations (3)
| Title |
|---|
| KAJ1296435.1;Sun, G.等;《GenBank》;第1页 * |
| Sun, G.等.KAJ1296435.1.《GenBank》.2022,1. * |
| Sun, G.等.KAJ1296435.1.《GenBank》.2022,第1页. * |
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