CN116898781A - Application of barley seedling exosomes in preparation of products with anti-aging repairing effect - Google Patents

Abstract

The invention discloses application of barley seedling exosomes in preparation of products with anti-aging repairing effects, and relates to the field of biotechnology. Barley seedling exosomes can improve the survival rate of cells under oxidative stress, promote cell proliferation, improve the antioxidant capacity of cells, reduce the MDA level and increase the SOD level; and can also improve the collagen synthesis capability of cells and promote the secretion of type I collagen and type III collagen. In the aspect of anti-aging repair, the barley seedling exosome and the adipose mesenchymal stem cell exosome have a certain synergistic effect.

Description

Application of barley seedling exosomes in the preparation of products with anti-aging and repairing effects

Technical field

The present invention relates to the field of biotechnology, and in particular to the application of barley seedling exosomes in the preparation of products with anti-aging and repairing effects.

Background technique

Exosomes are a type of extracellular vesicles secreted by cells. The diameter of plant exosomes is about 40-150nm. They are similar in morphology and structure to animal exosomes. They have a phospholipid bilayer structure and are saucer- or cup-shaped. They contain various plant chemical components and a large amount of lipids and microRNA inside. (miRNA), DNA and proteins, etc. The active substances contained in exosomes (especially miRNA) and their delivery are key links in maintaining intercellular communication. A large number of studies have proven that plant exosomes can realize cross-species material and information transfer from plants to animals.

Skin aging is a complex physiological process, in which peroxidative damage is one of the important causes of natural skin aging and photoaging. The most important cell component in the dermis of the skin is fibroblasts, and their reduction in number, morphological changes, and weakened or declining secretory and synthetic functions are closely related to skin aging. Studies have confirmed that damage to skin fibroblast function by peroxidation can lead to cell cycle changes, apoptosis, and abnormal collagen metabolism, leading to dry and rough skin, deepened wrinkles, weakened elasticity, and sagging. It is important to provide a drug that can effectively reduce oxidative damage and promote skin repair.

Contents of the invention

The present invention aims to solve at least one of the technical problems existing in the prior art. To this end, the present invention proposes the application of barley sprout exosomes in the preparation of products with anti-aging and repairing effects. Barley sprout exosomes can effectively improve the antioxidant capacity of cells, reduce cell oxidative damage, promote cell proliferation, and improve skin health. The ability of fibroblasts to secrete collagen.

The invention also provides an anti-aging repair composition.

The present invention also provides the application of the above anti-aging repair composition.

The present invention also provides products comprising the above anti-aging repair composition.

According to the first aspect of the present invention, the application of barley seedling exosomes in the preparation of products with anti-aging and repairing effects.

The anti-aging repair composition according to the embodiment of the present invention has at least the following beneficial effects:

The present invention found that barley seedling exosomes can improve the survival rate of cells under oxidative stress, promote cell proliferation, improve the antioxidant capacity of cells, reduce MDA levels, and increase SOD levels; and can also improve the collagen synthesis capacity of cells. Promote the secretion of type I collagen and type III collagen. Moreover, barley exosomes do not have a high effective dose, are difficult to ensure safety, and are easily hindered by the blood-brain barrier. The active ingredients in barley seedling exosomes are in natural soluble form, which solves the problem that many active ingredients are insoluble in water and have low physiological availability; and barley seedling exosomes are extremely stable. In addition to containing effective chemical ingredients, It also contains a variety of active ingredients and has a natural compound effect; its diameter is less than 150nm, and it has good penetration into various physiological barriers (such as blood-brain barrier and skin barrier).

According to some embodiments of the invention, the product is a cosmetic, a medicine or a kit.

According to some embodiments of the invention, the product further includes a cosmetically, pharmaceutically, chemically or biologically acceptable carrier.

An anti-aging repair composition according to a second embodiment of the present invention includes barley seedling exosomes and adipose mesenchymal stem cell exosomes.

The anti-aging repair composition according to the embodiment of the present invention has at least the following beneficial effects:

The anti-aging repair composition of the embodiment can promote cell proliferation, reduce oxidative damage to cells, and improve the antioxidant capacity and collagen secretion capacity of cells.

By utilizing the synergy between barley seedling exosomes and adipose mesenchymal stem cell exosomes, it is also possible to effectively avoid adipose mesenchymal stem cell exosomes caused by insufficient stem cell sources (especially stem cell sources from human living tissues). The problem of low acquisition rate can effectively reduce the usage of adipose mesenchymal stem cell exosomes and achieve better results.

According to some embodiments of the present invention, in terms of parts by mass, the composition includes 0.5-7 parts of barley seedling exosomes and 0.5-7 parts of adipose mesenchymal stem cell exosomes. For example, it may include 0.5, 1, 2, 3, 4, 5, 6 or 7 parts of barley sprout exosomes and 0.5, 1, 2, 3, 4, 5, 6 or 7 parts of adipose mesenchymal stem cell exosomes. body.

According to some embodiments of the present invention, the effects of the anti-aging repair composition include at least one of improving cellular antioxidant capacity and promoting cellular collagen secretion and production.

According to some embodiments of the present invention, promoting the secretion and production of cellular collagen includes promoting the secretion and production of type I collagen and/or type III collagen.

According to some embodiments of the present invention, improving cellular antioxidant capacity includes at least one of reducing MDA levels and increasing SOD levels.

According to some embodiments of the present invention, the adipose mesenchymal stem cell exosomes include human adipose mesenchymal stem cell exosomes.

According to some embodiments of the present invention, the anti-aging repair composition can improve the antioxidant capacity of cells. The anti-aging repair composition includes 1 part of barley seedling exosomes and 1 part of adipose mesenchymal stem cell exosomes. ; Or 1 part of barley seedling exosomes and 5 parts of adipose mesenchymal stem cell exosomes. Preferably, the effective concentration of the anti-aging repair composition is 1 μg/mL barley seedling exosomes and 1 μg/mL adipose mesenchymal stem cell exosomes; or 1 μg/mL barley seedling exosomes and 5 μg/mL Adipose mesenchymal stem cell exosomes.

According to some embodiments of the present invention, the anti-aging repair composition can improve the collagen secretion ability of cells. The anti-aging repair composition includes 1 part of barley seedling exosomes and 5 parts of adipose mesenchymal stem cell exosomes. exosomes; or 5 parts of barley seedling exosomes and 5 parts of adipose mesenchymal stem cell exosomes. Preferably, the effective concentration of the anti-aging repair composition is 1 μg/mL barley seedling exosomes and 5 μg/mL adipose mesenchymal stem cell exosomes; or 5 μg/mL barley seedling exosomes and 5 μg/mL Adipose mesenchymal stem cell exosomes.

According to some embodiments of the present invention, the method for preparing exosomes from barley seedlings includes the following steps: after squeezing the juice from barley seedlings, the exosomes are collected from the supernatant.

According to some embodiments of the present invention, a buffer solution needs to be added when the barley seedlings are juiced. The buffer solution is a buffer solution that is isotonic to the physiological environment. The buffer includes at least one of physiological saline, phosphate buffer, and Hank's buffer.

According to some embodiments of the present invention, the method for preparing exosomes from adipose mesenchymal stem cells includes the following steps: culturing the adipose mesenchymal stem cells, and culturing the adipose mesenchymal stem cells or their culture fluid. Exosomes were collected in clear medium.

According to some embodiments of the present invention, the method for collecting exosomes includes at least one of density gradient centrifugation, size exclusion chromatography, differential centrifugation, polyethylene glycol precipitation, immunoseparation, and screening separation. .

According to some embodiments of the present invention, the method of collecting exosomes includes differential centrifugation. The differential centrifugation method includes the following steps: centrifuge at 0-4°C, 200-400g for 5-20 minutes, and collect the first supernatant; centrifuge at 0-4°C, 1500-2500g for 10-30 minutes, and collect the second supernatant. liquid; centrifuge at 10000~14000g for 25~45min at 0~4°C, and collect the third supernatant; centrifuge at 10000~14000g for 25~45min at 0~4°C, and collect the fourth supernatant; at 0~4°C, Centrifuge at 130000~170000g for 70~90 min, discard the supernatant, and obtain exosomes.

According to the third embodiment of the present invention, the above-mentioned anti-aging repair composition is used in preparing a product with anti-aging repair effect. Since all the technical solutions of the anti-aging repair composition of the above embodiments are adopted, it has at least all the beneficial effects brought by the technical solutions of the above embodiments.

According to some embodiments of the present invention, the anti-aging repairing effect includes at least one of improving cellular antioxidant capacity and promoting cellular collagen secretion and production.

According to some embodiments of the present invention, promoting the secretion and production of cellular collagen includes promoting the secretion and production of type I collagen and/or type III collagen.

According to some embodiments of the present invention, improving cellular antioxidant capacity includes at least one of reducing MDA levels and increasing SOD levels.

A product with anti-aging and repairing effects according to the fourth embodiment of the present invention includes the anti-aging and repairing combination as described in the above-mentioned second embodiment. Since the product adopts all the technical solutions of the anti-aging repair combination of the above embodiments, it has at least all the beneficial effects brought by the technical solutions of the above embodiments.

According to some embodiments of the invention, the product is a cosmetic, a medicine or a kit.

According to some embodiments of the invention, the product further includes a cosmetically, pharmaceutically, chemically or biologically acceptable carrier.

According to some embodiments of the present invention, the dosage form of the product includes at least one of gel, emulsion, solution, gel, aerosol, powder, granule, capsule, and suspension.

According to some embodiments of the present invention, the anti-aging repair composition accounts for 1 to 99% of the total weight of the product.

Additional features and advantages of the invention will be set forth in the description which follows, and in part will be apparent from the description, or may be learned by practice of the invention.

Description of the drawings

Figure 1 is a TEM photograph of exosomes prepared in Examples 1 to 4 of the present invention.

Detailed ways

The concept of the present invention and the technical effects produced will be clearly and completely described below with reference to the embodiments, so as to fully understand the purpose, features and effects of the present invention. Obviously, the described embodiments are only some of the embodiments of the present invention, not all of them. Based on the embodiments of the present invention, other embodiments obtained by those skilled in the art without exerting creative efforts are all protection scope of the present invention.

If the specific conditions are not specified in the examples, the conditions should be carried out according to the conventional conditions or the conditions recommended by the manufacturer. If the manufacturer of the reagents or instruments used is not indicated, they are all conventional products that can be purchased commercially.

In the description of the present invention, if the first, second, third, fourth, etc. are described, they are only used for the purpose of distinguishing technical features, and cannot be understood as indicating or implying relative importance or implicitly indicating the indicated technology. The number of features or implicitly indicates the sequence relationship of the indicated technical features.

In the description of the present invention, the terms "including" and "having" and any variations thereof are intended to cover non-exclusive inclusion, for example, a process, method, system, product or equipment that includes a series of steps or units and is not necessarily limited to Those steps or elements that are expressly listed may instead include other steps or elements that are not expressly listed or that are inherent to the process, method, product or apparatus.

The complete culture medium used in the examples is DMEM/F12 medium containing 10% FBS and 1% double antibody.

Example 1

This embodiment provides a method for preparing barley seedling exosomes. The steps are as follows:

Cut fresh barley seedlings into small sections and grind them in a manual juicer to obtain barley seedling juice. Collect the juice; centrifuge the juice at 4°C and 300g for 10 minutes to collect the first supernatant; centrifuge at 4°C and 2000g for 20 minutes. Collect the second supernatant; centrifuge at 4°C, 12,000g for 35 minutes, collect the third supernatant, and repeat once; centrifuge at 4°C, 150,000g for 80 minutes, discard the supernatant, and add PBS to resuspend the pellet to obtain barley seedling exocytosis. body solution.

Example 2

This embodiment provides a method for preparing barley seedling exosomes. The steps are as follows:

Cut the fresh barley seedlings into small sections, add 5 times the weight of PBS, put it into a magnetic stirrer at 600 rpm for 60 minutes to obtain the barley seedling juice, and collect the juice; centrifuge the juice at 4°C, 300g for 10 minutes, and collect the first supernatant; Centrifuge at 2000g for 20 minutes at 4°C and collect the second supernatant; centrifuge at 12000g for 35 minutes at 4°C and collect the third supernatant and repeat once; centrifuge at 150000g for 80 minutes at 4°C and discard the supernatant and add PBS to resuspend. Precipitate to obtain a solution containing barley seedling exosomes.

Example 3

This embodiment provides a method for preparing barley seedling exosomes. The steps are as follows:

Cut the fresh barley seedlings into small sections, add 5 times the weight of PBS and squeeze the juice in a food processor to obtain the barley seedling juice. Collect the juice; centrifuge the juice at 300g for 10 minutes at 4°C and collect the first supernatant; , centrifuge at 2000g for 20 min, collect the second supernatant; centrifuge at 4°C, 12000g for 35 min, collect the third supernatant, repeat once; centrifuge at 4°C, 150000g for 80 min, discard the supernatant, add PBS to resuspend the pellet, and obtain Solution containing barley seedling exosomes.

Example 4

This embodiment provides a method for preparing exosomes from human adipose mesenchymal stem cells (hADSCs). The steps are as follows:

Inoculate human adipose mesenchymal stem cells into a culture bottle. When the cells grow to 80-90%, remove the culture medium, wash twice with PBS, add DMEM/F12 culture medium without exosomes, continue to culture for 48 hours, and collect Clear; centrifuge the supernatant at 4°C and 300g for 10 min to collect the first supernatant; centrifuge at 4°C and 2000g for 20 min to collect the second supernatant; centrifuge at 4°C and 12000g for 35 min to collect the third supernatant. 2 times; centrifuge at 150,000g for 80 minutes at 4°C, discard the supernatant, add PBS to resuspend the pellet, and obtain a solution containing hADSCs exosomes.

Example 5

This embodiment provides an anti-aging repair composition, consisting of 1 μg barley seedling exosomes and 5 μg hADSCs exosomes.

Example 6

This embodiment provides an anti-aging repair composition, consisting of 5 μg barley seedling exosomes and 5 μg hADSCs exosomes.

Example 7

This embodiment provides an anti-aging repair composition, consisting of 1 μg barley seedling exosomes and 1 μg hADSCs exosomes.

Example 8

This embodiment provides an anti-aging repair composition, consisting of 5 μg barley seedling exosomes and 1 μg hADSCs exosomes.

Example 9

This embodiment provides an anti-aging repair composition, which is 1 μg of barley seedling exosomes.

Example 10

This embodiment provides an anti-aging repair composition, which is 5 μg of barley seedling exosomes.

Example 11

This embodiment provides an anti-aging repair composition, which is 10 μg of barley seedling exosomes.

Comparative example 1

This comparative example provides an anti-aging repair composition, which is 1 μg hADSCs exosomes.

Comparative example 2

This comparative example provides an anti-aging repair composition, which is 5 μg hADSCs exosomes.

Comparative example 3

This comparative example provides an anti-aging repair composition, which is 10 μg hADSCs exosomes.

Comparative example 4

This comparative example provides an anti-aging repair composition, which is barley seedling extract. The extraction method is as follows:

Weigh a certain amount of barley seedlings, crush them, add 10 times the mass of distilled water, soak for 60 minutes, filter to remove impurities, and concentrate the filtrate under vacuum to obtain barley seedling extract.

Test example 1

This test example detects the morphology of barley seedling exosomes prepared in Examples 1 to 3 and hADSCs exosomes prepared in Example 4. The detection method is as follows:

Use a 10 μL pipette to accurately draw 10 μL of the exosome solution, drop it onto a 2 mm sample-loading copper grid, let it stand for 3 minutes at room temperature, and use clean filter paper to gently absorb the excess liquid from the edge of the sample-loading copper grid. After negative staining with 3% sodium phosphotungstate solution at room temperature for 5 minutes, wash gently with double-distilled water, dry at room temperature for 2 minutes, image on the machine (the operating voltage of the transmission scanning electron microscope is 80kv), and observe under the transmission electron microscope. Take photos.

The test results are shown in Figure 1.

Cup-shaped vesicles were observed in the barley seedling exosome solutions of Examples 1 and 2, but there were also a large number of impurities; the barley seedling exosome solution of Example 3 had fewer impurities and better morphology, consistent with exosomes. Cup-shaped vesicle morphology. Cup-shaped vesicles were also observed in the solution containing hADSCs exosomes in Example 4, which were significantly smaller in size than barley seedling vesicles.

Test example 2

1. This test example tests the antioxidant effect of the anti-aging repair compositions of Examples 5 to 11 and Comparative Examples 1 to 4.

The detection method is as follows:

(1) Place human skin fibroblasts (provided by Beina Biotechnology) in complete culture medium and culture them in a 37°C constant-temperature incubator with 5% _CO2 . Collect the cells in good growth status by trypsin digestion. Add 3mL of complete culture medium to each dish. Use a pipette to pipette the cells into a 5mL sterile centrifuge tube. Gently pipet the cells to mix them evenly. Pipette 20μL of the cell culture medium and place in it. In a 1.5mL centrifuge tube, add an equal volume of trypan blue solution, mix evenly, draw an appropriate amount and place it on a cell counting plate, count it with a fully automatic counter to obtain the number of cells, draw an appropriate amount of cell fluid and place it in a sample addition tank. Add an appropriate amount of complete culture medium, mix evenly, use a volley gun to absorb 100 μL of cell liquid and place it on a 96-well cell culture plate. The cell density is 6000 cells/well. Use the eight-figure or cross method to make it uniform, and place it in the incubator overnight to make it adhere. wall.

(2) After the cells adhere to the wall, discard the culture medium, add 600 μM H ₂ O ₂ and the anti-aging repair composition (see Table 1 for the final concentration of each embodiment or comparative example) to treat the cells for 24 hours, and record it as the experimental group; Cells without any treatment were used as the control group; cells treated with only 600 μM H ₂ O ₂ were used as the model group; complete culture medium without cells was used as the blank group; each group had 6 duplicate wells. After treatment, discard the culture medium, add 100 μL CCK-8 solution (CCK-8 stock solution: culture medium = 1:9), place it in the incubator and incubate for 40 minutes, measure the absorbance (OD value) of each well with a microplate reader, and detect the wavelength. It's 450nm.

Cell activity rate = (OD value of the experimental group - OD value of the blank group) / (OD value of the control group - OD value of the blank group) × 100%.

The experimental results are shown in Table 1.

Table 1

Treatment with barley seedling exosomes or hADSCs exosomes above 5 μg/mL can significantly inhibit oxidative damage caused by 600 μM H ₂ O ₂ and promote cell proliferation; combinations of barley seedling exosomes and hADSCs exosomes at different concentrations It can also significantly inhibit oxidative damage caused by 600μM H ₂ O ₂ and promote cell proliferation. Among them, there is a good synergistic effect between 1 μg/mL barley seedling exosomes and 1 μg/mL hADSCs exosomes, and between 1 μg/mL barley seedling exosomes and 5 μg/mL hADSCs exosomes, which can reduce oxidation. damage and promote cell proliferation.

2. SOD and MDA are the main landmark indicators for detecting oxidative stress damage. SOD is an important antioxidant enzyme in organisms. MDA is a lipid peroxide formed by polyunsaturated fatty acids attacked by oxygen free radicals. It can reflect the degree of lipid peroxidation in the body and indirectly reflect the degree of cell damage. This test example tests the effects of the anti-aging repair compositions of Examples 5 to 10 and Comparative Examples 1 to 2 on superoxide dismutase (SOD) and malondialdehyde (MDA) of human skin fibroblasts.

The detection method is as follows:

Cells were seeded in a 24-well plate at a seeding density of 3×10 ⁴ cells/well and cultured overnight to allow cells to adhere to the wall. Each well was treated with different anti-aging repair compositions (see Table 1 for final concentration) and 600 μM H ₂ O ₂ . Add 600 μM H ₂ O ₂ to the model group wells and treat for 8 hours. Discard the cell culture medium and wash with PBS for 2 hours. Then, add 200 μL of PBS, scrape off the cells with a cell scraper, use a pipette to absorb the cell fluid and place it in a 1.5mL centrifuge tube, add grinding beads, then balance it and place it in a ball mill for grinding for 300 seconds, centrifuge at 4°C and 12000rpm for 5 minutes. Take the supernatant and place it in another centrifuge tube, centrifuge again, take the supernatant and mix evenly to obtain the sample to be tested. The MDA assay kit (WLA048, provided by Shenyang Wanlei Biotechnology Co., Ltd.) was used to detect the MDA level, and the BCA kit was used to determine the sample protein concentration. The operation should be carried out strictly according to the instructions.

Cells were seeded in a 24-well plate at a seeding density of 3×10 ⁴ cells/well and cultured overnight to allow cells to adhere to the wall. Different anti-aging repair compositions (see Table 2 for final concentration) and 600 μM H ₂ O _{2 were added to each well. 600 μM H 2 O 2 was added} to the model group wells and treated for 8 hours. The cell culture medium was discarded and washed with PBS for 2 hours. Second-rate. The total SOD activity detection kit (S0101, provided by Shanghai Beyuntian Biotechnology Co., Ltd.) was used to detect SOD enzyme activity units, and the BCA kit was used to determine the protein concentration. The operation should be carried out strictly according to the instructions.

The test results are shown in Table 2.

Table 2

Barley seedling exosomes, hADSCs exosomes or their combination can effectively reduce MDA levels and increase SOD levels. Among them, there is a significant synergistic effect between 1 μg/mL barley seedling exosomes and 5 μg/mL hADSCs exosomes, which can effectively improve the antioxidant capacity of human skin fibroblasts.

3. This test example tests the effect of the anti-aging repair compositions of Examples 5 to 10 and Comparative Examples 1 to 2 on the collagen synthesis ability of human skin fibroblasts.

The test method is as follows:

Cells were seeded in a 24-well plate at a seeding density of 3×10 ⁴ cells/well and cultured overnight to allow cells to adhere to the wall. Each well was treated with different anti-aging repair compositions (see Table 3 for final concentration) and 600 μM H ₂ O ₂ . Add 600 μM H ₂ O ₂ to the model group wells and treat for 8 hours. Discard the cell culture medium and wash with PBS for 2 hours. times, add 500 μL of Trizol, extract total RNA, reverse-transcribe to obtain cDNA, use SYBR Green method to perform qPCR, and calculate the relative value of the target gene according to the 2-△△Ct method, △△Ct=(Ct target gene-Ct GAPDH) experiment Group-(Ct target gene-Ct GAPDH) control group.

Among them, the upstream primer used to detect the expression of type I collagen is 5'-TACAGCGTCACTGTCGATGGC-3', and the downstream primer is 3'-TCAATCACTGTCTTGCCCCAG-5'; the upstream primer used to detect the expression of type III collagen is 5'- AATTTGGTGTGGACGTTGGC-3', the downstream primer is 3'-TTGTCGGTCACTTGCACTGG-5'; the internal reference is GAPDH, the upstream primer used for detection is 5'-CAACAGCGACACCCACTCCT-3', and the downstream primer is 3'-CACCCTGTTGCTGTAGCCAAA-5'.

The reaction conditions of qPCR were: 95°C for 2 min; 95°C for 15 s, 60°C for 30 s, and 72°C for 30 s, 40 cycles.

The experimental results are shown in Table 3.

table 3

Barley seedling exosomes, hADSCs exosomes or their combination can effectively promote the synthesis of type I collagen and type III collagen by human skin fibroblasts. Among them, there is a significant synergistic effect between 1 μg/mL barley seedling exosomes and 5 μg/mL hADSCs exosomes, and 5 μg/mL barley seedling exosomes and 5 μg/mL hADSCs exosomes, which can effectively improve human skin composition. The ability of fibroblasts to synthesize type I collagen and type III collagen improves the skin repair effect.

The embodiments of the present invention have been described in detail above with reference to the accompanying drawings. However, the present invention is not limited to the above embodiments. Within the scope of knowledge possessed by those of ordinary skill in the art, various modifications can be made without departing from the purpose of the present invention. Variety.

Claims (10)

1. The application of barley seedling exosomes in preparing products with anti-aging repairing effect.

2. An anti-aging repair composition, which is characterized by comprising barley seedling exosomes and adipose mesenchymal stem cell exosomes.

3. The anti-aging repair composition according to claim 2, wherein the composition comprises 0.5 to 7 parts by mass of barley seedling exosomes and 0.5 to 7 parts by mass of adipose mesenchymal stem cell exosomes.

4. An anti-ageing repair composition according to claim 3, wherein the preparation method of barley seedling exosomes comprises the following steps: squeezing barley seedling, and collecting exosomes from supernatant;

preferably, the method of collecting exosomes comprises at least one of density gradient centrifugation, size exclusion chromatography, differential centrifugation, polyethylene glycol precipitation, immunoisolation, screening separation.

5. Use of an anti-ageing repair composition as claimed in any of claims 2 to 4 for the preparation of a product having anti-ageing repair action.

6. The use of claim 5, wherein the anti-aging repair comprises at least one of increasing cellular antioxidant capacity and promoting cellular collagen secretion production.

7. The use of claim 6, wherein promoting cellular collagen secretion production comprises promoting type I collagen and/or type III collagen secretion production;

preferably, increasing the antioxidant capacity of the cell comprises at least one of decreasing MDA levels, increasing SOD levels.

8. A product having an anti-ageing repair effect, characterized by comprising an anti-ageing repair composition as claimed in any of claims 2 to 4.

9. The product of claim 8, further comprising a cosmetically, pharmaceutically, chemically, or biologically acceptable carrier.

10. The product of claim 8, wherein the anti-aging repair composition comprises 1 to 99% by weight of the total product.