CN116784226A - A method of breeding sugarcane varieties with strong ratoon roots - Google Patents

Abstract

The invention relates to the technical field of agricultural planting, and discloses a method for breeding sugarcane varieties with strong ratoon properties. The main steps are to first select female and male sugarcane parents with strong ratoon properties for hybridization, and use multifunctional scissors to cut the required male parents. , and then carry out pollination operations. After the hybridization is completed, the individual sugarcane plants obtained from the hybridization are cultivated. In the first four years, lines infected with important diseases in the natural environment are eliminated. In five to six years, the hybrid sugarcane lines are artificially inoculated with smut resistance. Identification, within seven to ten years, the remaining hybrid sugarcane lines with strong ratoon and disease resistance will be carried out in regional trials and production trials to accelerate breeding, and finally the varieties will be registered. By using multifunctional scissors, the present invention can also irrigate the cutting opening with a certain amount of culture fluid when cutting the male parent sugar cane, thereby preserving the optimal state of the male parent's male flowers to a certain extent and improving the pollination rate and seed setting rate.

Description

A method of breeding sugarcane varieties with strong ratoon roots

Technical field

The invention relates to the technical field of agricultural planting, and in particular to a method for breeding sugarcane varieties with strong ratoon roots.

Background technique

As an important sugar and bioenergy crop, sugarcane can save seedlings, save labor, and advance maturity by retaining ratoon roots. It can also help speed up the promotion of improved seeds, reduce the planting cycle, and extend the production cycle. Under the same management conditions, the quality and sugar yield of ratoon cane of the same sugarcane variety are higher than those of newly planted cane. Therefore, the breeding of sugarcane varieties with strong ratoon occupies an extremely important position in production and is valued by major sucrose-producing countries.

The ratoon nature of sugarcane and its yield are not only affected by cultivation techniques and environmental conditions, but also the ratoon nature of the variety itself plays a key role. In the selection of new sugarcane varieties, in addition to factors such as yield, sugar content, lodging resistance, etc., the ratoon nature of the variety itself plays a key role. Root property is also an extremely important breeding factor. Breeding excellent sugarcane varieties with strong ratoon properties can increase ratoon yield and extend ratoon life, which plays a very important role in high, stable and efficient sugarcane production. Currently, most sugarcane seeds are directly planted Cultivation is carried out among various nurseries, but the cultivated sugarcane seeds are not selected, so that a large number of seeds will be eliminated during the cultivation. Therefore, the quality of the sugarcane seeds is first achieved through hybridization to a better level. , and then cultivate ratoons, thereby increasing the amount of high-quality seeds obtained from cultivation.

Contents of the invention

The present invention is intended to provide a method for breeding sugarcane varieties with strong ratoon roots, so as to solve the problem of a large number of eliminated sugarcane seeds in the existing breeding methods.

In order to achieve the above object, the basic scheme of the present invention is as follows: a method for breeding strong ratoon sugarcane varieties, including the following steps:

Step 1: Select the female and male parents of sugarcane with strong ratoon properties, and achieve hybrid cultivation of the female and male parents through artificial pollination. The pollination process is to place the female parent in a cage surrounded by cloth, and then use multiple Use functional scissors to cut the male sugar cane. At the same time, before the male male flower pollen is scattered, the male parent is placed in the same cloth cage as the female parent, and the cut of the male sugar cane is placed in 6% sulfurous acid culture. In the liquid, keep the male parent in the cage until all the male parent have finished flowering. The multi-functional scissors can spray the sulfurous acid culture solution when cutting the male parent to improve the male flower vitality of the male parent and the seed setting rate of the female parent;

Step 2: Cultivate the sugarcane seeds obtained after hybridization. First, sow the sugarcane seeds in sterilized soil, cover with a thin layer of soil, then spray water until wet, cover with a film to keep warm and moisturizing, remove the film after the seedlings emerge, and wait until the seedlings grow to After 4 leaves, start planting into plug trays, and transplant to field planting after 35 to 40 days;

Step 3: In the first four years of the first four years of hybrid sugarcane individual plants, sugarcane smut, sugarcane rust or sugarcane mosaic lines that have reached the S level of susceptibility to disease are eliminated;

Step 4: Within five to six years of continued observation, individual hybrid sugarcane plants will be identified for smut resistance using artificial inoculation methods, and sugarcane smut resistance levels will be selected to reach disease resistance level R or above and have strong ratoon resistance. hybrid sugarcane strains,

Step 5: At seven to ten years, accelerated breeding and regional trials of high-quality hybrid sugarcane lines will be carried out, and sugarcane lines with strong ratoon properties and disease resistance will be selected for variety registration.

Further, in step one, the multifunctional scissors include a first scissor handle and a second scissor handle. The first scissor handle and the second scissor handle are both fixedly connected with blades, and the blades are provided with several grooves. The first scissor handle and the second scissors handle are fixedly connected to each other. The two scissor handles are hinged and there is a gap between the hinge joint of the first scissor handle and the second scissor handle and the blade. An elastic water storage ball is fixedly connected to the first scissor handle, and a shunt tube is connected to the water storage ball. The shunt tube is bonded to both sides of the gap.

Furthermore, in step two, the soil selected for seedling cultivation needs to be sterilized with steam for 2 hours to kill soil pathogens and weed seeds, and the sowing density is controlled to 150 seedlings within 30cm×30cm, and the temperature is controlled at 25~ 30℃, and spray water 2 to 3 times every morning and afternoon. When the seedlings have 4 leaves, they are planted in plug trays and transplanted to the field after 35 to 40 days.

Furthermore, in the third step, hybrid sugarcane seeds are cultivated in the hybrid nursery in the first year. Single plants are planted without selection, and ratoons are retained for selection in the second year. A control variety with strong ratoon properties is set to select those with excellent comprehensive traits. A single plant enters the third year of testing; in the third year, the selected sugarcane lines are planted in a single row without duplication. The row length is 5m. They are newly planted for one year without retaining ratoons. A control variety is set up to select sugarcane lines with excellent comprehensive traits. Entering the fourth year of testing; in the fourth year, the selected sugarcane lines are planted in rows 2 to 4 without duplication, with new planting for one year and ratoon roots retained for one year;

Set up a control variety with strong ratoon resistance, and conduct field identification of the natural incidence rates of sugarcane smut, sugarcane rust and sugarcane mosaic disease on the planted sugarcane lines. Eliminate the susceptible S-level lines and select those with strong ratoon resistance. Lines with excellent comprehensive traits will enter the next year's test.

Further, in step 4, in the fifth year, a preliminary product comparison test of hybrid sugarcane strains is carried out. The excellent sugarcane strains selected in the previous year are repeated three times and planted in a 4-row area. The plot area is 0.05 acres, and 1 ~Two control varieties with strong ratoon properties, which are newly planted for one year and the ratoons are retained for one year. smut spore powder is collected and artificially inoculated with sugarcane smut by dipping method. Sugarcane lines with disease resistance above R level are selected to enter the next step. year test;

Between the sixth and seventh years, conduct a variety comparison test on the hybrid sugarcane lines selected in the previous year, set up 1 to 2 control varieties with strong ratoon properties, and repeat the sugarcane lines 3 to 4 times and plant them in rows 4 to 5 In the area, the area of the plot is 0.05 to 0.1 acres, of which new plantings are planted for one year and ratoons are retained for one year. Sugarcane lines with strong ratoon properties and excellent comprehensive traits are selected for experimentation and propagation.

Further, in step five, in the seventh to ninth years, conduct regional trials on hybrid sugarcane strains, select 8 to 10 regional trial test points, and plant the selected hybrid sugarcane strains three times in rows 4 to 5 of the plot. The area is 0.05 to 0.1 acres, and 1 to 2 control varieties with a large local planting area are set up. The new planting lasts for one year and the ratoon roots are retained for two years. Sugarcane lines whose comprehensive characteristics are better than those of the local control varieties are selected for rapid propagation;

In the ninth to tenth year, the sugarcane lines selected through the regional trial test will be tested for production. The sugarcane lines will be planted in a test area with an area of 1 to 2 acres to observe their group performance, ratooning and natural incidence rates. Agronomic traits include one year for new planting and one year for retaining ratoons. Excellent sugarcane lines with strong ratoon properties and disease resistance are selected for variety registration.

The following beneficial effects have been achieved after adopting the above solution:

1. First, after hybridizing sugarcane seeds, relatively high-quality seeds can be obtained. Then, through the cultivation of hybrid sugarcane seedlings in the first three years, some hybrid sugarcane seeds that cannot adapt to the disease can be eliminated, thereby achieving Preliminary screening of hybrid sugarcane seeds, and then further screening of hybrid sugarcane seedlings at four to six years to eliminate some strains that are unable to resist smut, rust, and mosaic diseases, and then eliminate some strains that cannot resist smut, rust, and mosaic diseases, and then conduct further screening of hybrid sugarcane seedlings at four to six years. At this time, relatively high-quality hybrid sugarcane strains can be selected. Through ten years of selection, relatively high-quality hybrid sugarcane strains can be cultivated to enhance the yield and quality of sugarcane.

2. During pollination, the flowers of the female parent and the male parent need to be placed in cages. This can avoid external wind factors from affecting the hybridization results of sugarcane to a certain extent, and maximize the effect of hybridization while pruning. When using the male parent, squeeze the first scissor handle and the second scissor handle with your hands to bring the first scissor handle and the second scissor handle together. When the first scissor handle and the second scissor handle are closed, on the one hand, the blades can be closed and To achieve the shearing action, on the other hand, the water storage ball will be squeezed. When the water storage ball is under pressure, it will deform, allowing the culture fluid in the water storage ball to flow to the blade through the shunt tube, thereby achieving edge shearing. While pouring the culture medium on it, when the first scissor handle and the second scissor handle are opened, the pressure on the water storage ball decreases, the water storage ball will rebound, and a certain suction force will be generated while rebounding, thereby pulling the blade Part of the culture solution on the blade is absorbed back to avoid the waste of culture solution. At the same time, when the culture solution is sprayed on the blade, due to the groove design on the blade, a certain interception effect can be produced, so that part of the culture solution stays on the surface of the blade. When cutting the male parent, you can apply culture solution to the incision of the male flower to ensure the vigorous state of the male flowers. At the same time, it can avoid a large amount of culture solution flowing to the ground when spraying the culture solution on the blade, causing waste.

Description of the drawings

Figure 1 is a scissors front view of the breeding method for strong ratoon sugarcane varieties in the embodiment of the present invention.

Figure 2 is a schematic diagram of the sugarcane hybridization method of the breeding method for strong ratoon sugarcane varieties in the embodiment of the present invention.

Detailed ways

The following is further detailed through specific implementation methods:

The reference numerals in the drawings of the description include: first scissor handle 1, water storage ball 2, shunt tube 3, groove 4, second scissor handle 5, and blade 6.

The embodiment is basically as shown in Figures 1-2: a method for breeding strong ratoon sugarcane varieties, including the following steps:

Step 1: Select the female and male parents of sugarcane with strong ratoon properties, and achieve hybrid cultivation of the female and male parents through artificial pollination. The pollination process is to place the female parent in a cage surrounded by cloth, and then use multiple Use functional scissors to cut the male sugar cane. At the same time, before the male male flower pollen is scattered, the male parent is placed in the same cloth cage as the female parent, and the cut of the male sugar cane is placed in 6% sulfurous acid culture. In the liquid, keep the male parent in the cage until all the male parent have finished flowering. The multi-functional scissors can spray the sulfurous acid culture solution when cutting the male parent to improve the male flower vitality of the male parent and the seed setting rate of the female parent; The multifunctional scissors include a first scissor handle 1 and a second scissor handle 5. The first scissor handle 1 and the second scissor handle 5 are both fixedly connected with a blade 6. The blade 6 is provided with a number of grooves 4. The first scissor handle 1 It is hinged to the second scissor handle 5 and there is a gap between the hinged joint of the first scissor handle 1 and the second scissor handle 5 and the blade 6. The first scissor handle 1 is fixedly connected with an elastic water storage ball 2, and the water storage ball 2 is connected to There is a shunt pipe 3, which is bonded to both sides of the gap. As shown in Figure 2, the top is the male parent and the bottom is the female parent.

Step 2: Cultivate the sugarcane seeds obtained after hybridization. First, sow the sugarcane seeds in sterilized soil, cover with a thin layer of soil, then spray water until wet, cover with a film to keep warm and moisturizing, remove the film after the seedlings emerge, and wait until the seedlings grow to After 4 leaves, start planting into plug trays, and transplant to field planting after 35 to 40 days;

Among them, the soil selected for seedling cultivation needs to be sterilized with steam for 2 hours to kill soil pathogens and weed seeds, and the sowing density is controlled to 150 seedlings within 30cm × 30cm. At the same time, the temperature is controlled between 25 and 30°C, and It is necessary to spray water 2 to 3 times in the morning and afternoon every day. When the seedlings have 4 leaves, they are planted in plug trays and transplanted to the field after 35 to 40 days.

Step 3: In the first four years, individual hybrid sugarcane plants are eliminated from sugarcane smut, sugarcane rust or sugarcane mosaic lines that reach the S level of susceptibility under natural conditions; the resistance levels of sugarcane plants to sugarcane smut are respectively Highly resistant (HR), resistant 1 (R1), resistant (R2), moderately resistant (MR), moderately susceptible (MS), susceptible 1 (S1), susceptible 2 (S2), highly susceptible 1 (HS1) ) and High Sense 2 (HS2);

The resistance levels of sugarcane plants to sugarcane rust are highly resistant (HR), resistant (R), moderately resistant (MR), moderately susceptible (MS), susceptible 1 (S1), susceptible 2 (S2), and susceptible. Sickness 3 (S3), High Susceptibility 1 (HS1) and High Susceptibility 2 (HS2);

The resistance levels of sugarcane plants to sugarcane mosaic disease are immune (I), highly resistant (HR), moderately resistant (MR), susceptible (S) and highly susceptible (HS).

Specifically, hybrid sugarcane seeds are cultivated in the hybrid nursery in the first year, and individual plants are planted without selection. Keep the ratoons until selection in the second year, set up a control variety with strong ratoon properties, and select individual plants with excellent comprehensive traits to enter the third year of testing; in the third year, the selected sugarcane lines are planted in single rows without duplication, and the row length is 5m, newly planted for one year, without retaining ratoons, setting up a control variety, and selecting sugarcane lines with excellent comprehensive characteristics to enter the fourth year of testing; in the fourth year, the selected sugarcane lines will be planted in rows 2 to 4 without duplication. Newly plant for one year and retain ratoon roots for one year;

Set up a control variety with strong ratoon resistance, and conduct field identification of the natural incidence rates of sugarcane smut, sugarcane rust and sugarcane mosaic disease on the planted sugarcane lines. Eliminate the susceptible S-level lines and select those with strong ratoon resistance. Lines with excellent comprehensive traits will enter the next year's test.

Step 4: Within five to six years of continued observation, individual hybrid sugarcane plants will be identified for smut resistance using artificial inoculation methods, and sugarcane smut resistance levels will be selected to reach disease resistance level R or above and have strong ratoon resistance. hybrid sugarcane lines;

Specifically, a preliminary comparison test of hybrid sugarcane strains will be carried out in the fifth year. The excellent sugarcane strains selected in the previous year will be planted three times in a 4-row area. The area of the plot is 0.05 acres, and 1 to 2 controls with strong ratoon properties will be set up. The varieties are newly planted for one year and ratoons are retained for one year. Smut spore powder is collected and artificially inoculated with sugarcane smut by dipping method. Sugarcane lines with disease resistance level R and above are selected to enter the next year's test;

Between the sixth and seventh years, conduct a variety comparison test on the hybrid sugarcane lines selected in the previous year, set up 1 to 2 control varieties with strong ratoon properties, and repeat the sugarcane lines 3 to 4 times and plant them in rows 4 to 5 In the area, the area of the plot is 0.05 to 0.1 acres, of which new plantings are planted for one year and ratoons are retained for one year. Sugarcane lines with strong ratoon properties and excellent comprehensive traits are selected for experimentation and propagation.

Step five: at seven to ten years, conduct accelerated propagation and regional trials of high-quality hybrid sugarcane lines, and select sugarcane lines with strong ratoon properties and disease resistance for variety registration;

Specifically, in the seventh to ninth years, regional trials of hybrid sugarcane strains will be carried out, 8 to 10 regional trial test points will be selected, and the selected hybrid sugarcane strains will be planted three times in 4 to 5 row areas, with a plot area of 0.05 to 0.1 mu, set up 1 to 2 control varieties with a large local planting area, of which new planting will take one year and ratoon roots will be retained for two years. Sugarcane lines with comprehensive properties better than the local control varieties will be selected for rapid breeding;

In the ninth to tenth year, the sugarcane lines selected through the regional trial test will be tested for production. The sugarcane lines will be planted in a test area with an area of 1 to 2 acres to observe their group performance, ratooning and natural incidence rates. Agronomic traits include one year for new planting and one year for retaining ratoons. Excellent sugarcane lines with strong ratoon properties and disease resistance are selected for variety registration.

To sum up, in this embodiment, after first hybridizing sugarcane seeds, relatively high-quality seeds can be obtained, and then through the cultivation of hybrid sugarcane seedlings in the first four years, some of the sugarcane seedlings that cannot adapt to the disease can be Hybrid sugarcane seeds are eliminated to achieve preliminary screening of hybrid sugarcane seeds. Then, in five to six years, hybrid sugarcane seedlings are further screened to eliminate some lines that are unable to resist smut, rust, and mosaic diseases. Through elimination, higher-quality hybrid sugarcane strains can be selected in seven to ten years. Through ten years of selection, higher-quality hybrid sugarcane strains can be cultivated to enhance the yield and quality of sugarcane.

During pollination, the flowers of the female parent and the male parent need to be placed in cages to avoid external wind factors from affecting the hybridization results of sugarcane to a certain extent, and to maximize the effect of hybridization. At the same time, the male parent is cut When the first scissor handle 1 and the second scissor handle 5 are squeezed by hand, the first scissor handle 1 and the second scissor handle 5 are closed. When the first scissor handle 1 and the second scissor handle 5 are closed, on the one hand, The blade 6 is closed and sheared. On the other hand, the water storage ball 2 is squeezed. The water storage ball 2 will deform under pressure, causing the culture medium in the water storage ball 2 to flow through the shunt tube 3 to The blade 6 is used to cut the male parent while pouring culture medium on it. At the same time, when the first scissor handle 1 and the second scissor handle 5 are opened, the pressure on the water storage ball 2 is reduced, and the water storage ball 2 will rebound. While rebounding, a certain suction force will be generated, which will absorb part of the culture solution on the blade 6 back to avoid the waste of culture solution. At the same time, when spraying the culture solution on the blade 6, due to the design of the groove 4 on the blade 6, It can produce a certain interception effect, so that part of the culture solution stays on the surface of the blade 6. When cutting the male flower, the culture solution can be applied to the incision of the male flower to ensure the vigorous state of the male flower, and at the same time, it can avoid spraying the culture solution on the blade 6. When the culture liquid is removed, a large amount of culture liquid flows to the ground, causing waste.

The above are only embodiments of the present invention, and common knowledge such as well-known specific structures and/or characteristics of the solutions will not be described in detail here. It should be pointed out that for those skilled in the art, several modifications and improvements can be made without departing from the structure of the present invention. These should also be regarded as the protection scope of the present invention and will not affect the implementation of the present invention. effectiveness and patented practicality. The scope of protection claimed in this application shall be based on the content of the claims, and the specific implementation modes and other records in the description may be used to interpret the content of the claims.

Claims (6)

1. A breeding method of a strong ratoon sugarcane variety is characterized by comprising the following steps: comprises the steps of,

firstly, selecting a sugarcane female parent and a male parent with strong ratoon property, realizing hybridization cultivation of the female parent and the male parent by means of artificial pollination, wherein the pollination process is to put the female parent into a cage surrounded by cloth, then shear the male parent sugarcane by using a multifunctional scissors, simultaneously put the male parent into the cage of the same cloth as the female parent before pollen of male flowers of the male parent is scattered, put the incision of the male parent sugarcane into a 6% sulfurous acid culture solution, and put the male parent into the cage until all the male parent is completely bloomed, wherein the multifunctional scissors can spray the sulfurous acid culture solution when shearing the male parent, so that the male flower vigor of the male parent and the fruiting rate of the female parent are improved;

step two, raising seedlings of the sugarcane seeds obtained after hybridization, sowing the sugarcane seeds in sterilized soil, covering Bao Tu, spraying water until the sugarcane seeds are wet, covering a film for heat preservation and moisture preservation, removing the film after seedling emergence, starting temporary planting on a plug tray after seedlings grow to 4 leaves, and transplanting to a field for planting after 35-40 days;

step three, eliminating sugarcane smut, rust disease or mosaic disease strains of the sugarcane reaching the disease-sensing S level under natural conditions in the first four years;

step four, the resistance identification of the smut is carried out on the single hybrid sugarcane plants in five to six years of continuous observation by using a manual inoculation method, and the hybrid sugarcane strain with the resistance level of the sugarcane smut reaching the disease resistance level above R and strong ratoon is selected;

and fifthly, performing accelerated propagation and region test on the high-quality hybrid sugarcane strain in seven to ten years, and selecting the sugarcane strain with strong ratoon property and disease resistance for variety registration.

2. The method for breeding a strong ratoon sugarcane variety according to claim 1, which is characterized in that: in the first step, the multifunctional scissors comprise a first scissors handle and a second scissors handle, the first scissors handle and the second scissors handle are fixedly connected with cutting edges, a plurality of grooves are formed in the cutting edges, the first scissors handle and the second scissors handle are hinged, gaps are formed between the hinged positions of the first scissors handle and the second scissors handle and the cutting edges, an elastic water storage ball is fixedly connected to the first scissors handle, a shunt tube is communicated to the water storage ball, and the shunt tube is adhered to two sides of the gaps.

3. The method for breeding a strong ratoon sugarcane variety according to claim 2, which is characterized in that: in the second step, the selected seedling raising soil needs to be sterilized by steam for 2 hours to kill soil germs and weed seeds, the sowing density is controlled to be preferably 150 seedlings within 30cm multiplied by 30cm, the temperature is controlled to be between 25 and 30 ℃, water is sprayed for 2 to 3 times in the morning and afternoon every day, 4 leaves of the seedlings are temporarily planted on a plug tray, and the seedlings are transplanted to a field for 35 to 40 days.

4. The method for breeding a strong ratoon sugarcane variety according to claim 3, wherein the method comprises the following steps: in the third step, the first year hybrid sugarcane seeds are cultivated in a hybrid garden, single plants are planted without selection, perennial roots are reserved for selection in the second year, 1 control variety with strong perennial root property is arranged, and single plants with excellent comprehensive properties are selected and selected to enter a third year test; in the third year, single-row non-repeated planting is carried out on the selected sugarcane strain, the row length is 5m, the new planting is carried out for one year, no perennial roots are reserved, 1 control variety is arranged, and the sugarcane strain with excellent comprehensive properties is selected to enter a fourth year test; the selected sugarcane strain is planted in 2-4 rows for a fourth year without repetition, is planted for one year newly, and is reserved for one year;

setting 1 control variety with strong ratoon property, identifying the natural incidence rate of sugarcane smut, sugarcane rust and sugarcane mosaic disease in the field on the planted sugarcane strain, eliminating the strain with S level of susceptibility, selecting the strain with strong ratoon property and excellent comprehensive properties, and entering the next year test.

5. The method for breeding a strong ratoon sugarcane variety according to claim 4, wherein the method comprises the following steps: in the fourth step, a preliminary product ratio test of hybrid sugarcane strains is carried out in the fifth year, the excellent sugarcane strains selected in the last year are repeatedly planted in 4 rows of areas for three times, the area of each area is 0.05 mu, 1-2 control varieties with strong ratoon property are arranged, the ratoon is newly planted for one year, the ratoon is reserved for one year, smut spore powder is collected for artificial inoculation of sugarcane smut by an immersion method, and sugarcane strains with disease resistance R grade above are selected to enter the next year test;

and (3) in the sixth year to seventh year, performing a variety comparison test on the hybrid sugarcane strain selected in the last year, setting 1-2 control varieties with strong ratoon property, repeatedly planting the sugarcane strain in a 4-5 row area for 3-4 times, wherein the area of the area is 0.05-0.1 mu, newly planting for one year, reserving ratoon for one year, and selecting the sugarcane strain with strong ratoon property and excellent comprehensive properties for propagation while performing the test.

6. The method for breeding the strong ratoon sugarcane varieties according to claim 5, which is characterized in that: in the fifth step, in the seventh to nine years, regional test is carried out on the hybrid sugarcane strain, 8 to 10 regional test points are selected, the selected hybrid sugarcane strain is repeatedly planted in 4 to 5 rows of regions, the area of the region is 0.05 to 0.1 mu, 1 to 2 control varieties with large local planting areas are arranged, one year of new planting is carried out, perennial roots are reserved for two years, and the sugarcane strain with comprehensive characters superior to those of the local control varieties is selected for rapid propagation;

in the ninth to ten years, the production test is carried out on the sugarcane strain selected through the district test, the sugarcane strain is planted in a test district with the district area of 1-2 mu, the group performance, ratoon nature and natural morbidity agronomic character are observed, the new planting is carried out for one year, ratoon is reserved for one year, and the excellent sugarcane strain with strong ratoon nature and disease resistance is selected for variety registration.