CN116584652A - A kind of nanoscale DHA algae oil freeze-dried instant tablet and preparation method thereof - Google Patents

Abstract

The invention discloses a nano-scale DHA algae oil freeze-dried instant tablet and a preparation method thereof. Wherein, a kind of nanoscale DHA algae oil freeze-dried instant tablet, its raw material comprises: DHA algae oil 10-40 parts, protein 5-15 parts, lyoprotectant 40-70 parts, stabilizer 0.1 parts by weight. ‑5 parts, emulsifier 0.1‑5 parts, flavoring agent 0‑0.2 parts, purified water 285‑667 parts. The invention discloses a nano-scale DHA algae oil freeze-dried instant tablet and a preparation method thereof, which has good flavor, good taste, convenient eating, easy absorption and high oil loading capacity, and can better maintain the original emulsion particles after rehydration diameter, improving absorption and bioavailability.

Description

A kind of nanoscale DHA algae oil freeze-dried instant tablet and preparation method thereof

technical field

The invention relates to the technical field of food processing, in particular to a nano-scale DHA algae oil freeze-dried instant tablet and a preparation method thereof.

Background technique

DHA, scientific name docosahexaenoic acid, commonly known as brain gold. It is an Omega-3 unsaturated fatty acid that is very beneficial to the human body. DHA is a main element for the growth and maintenance of cells in the nervous system. It is an important constituent fatty acid of the brain and retina. The content of DHA in the human cerebral cortex is as high as 20%, and it is very important for the development of intelligence and vision.

The recommended daily intake of DHA is not higher than 300mg, and the existing DHA products mainly exist in the form of gel candy, soft capsule, solid drink, tablet candy, etc. Since DHA is mainly derived from algae oil, it will have a certain fishy smell. Direct intake of algae oil will bring a greasy feeling and affect the acceptability of the human body. Most of the algae oils in existing products are directly added to candies or soft capsules, which have defects such as insufficient absorption, poor taste, and unacceptable greasy.

Nano-food refers to food that adopts nanotechnology in the process of production, processing or packaging. From a narrow perspective, only products that use nanotechnology to transform and process food ingredients themselves can be called nano-food. Nanoemulsions are generally thermodynamically stable, isotropic, transparent or translucent homogeneous dispersion systems that can be used to carry lipids, thereby improving the absorption rate in the digestive tract and increasing bioavailability. DHA nanoemulsions will break and decompose when placed at room temperature for a long time. Making nanoemulsions into dry emulsions can significantly improve their stability at room temperature. Rehydration may cause droplets to aggregate and particle size to change, which is not conducive to absorption and needs to be improved.

Contents of the invention

Aiming at the problem that the rehydration of nano-scale DHA algae oil in the prior art may cause droplets to aggregate, and the acceptability of the human body is poor, the purpose of the present invention is to provide a nano-scale DHA algae oil freeze-dried instant tablet and its preparation The method has the advantages of good flavor, good taste, convenient consumption, easy absorption and high oil loading capacity. After rehydration, the particle size of the original emulsion can be better maintained, and the absorption rate and bioavailability are improved.

In order to achieve the above object, the present invention provides the following technical scheme: a nano-scale DHA algae oil freeze-dried instant tablet, in parts by weight, its raw materials include: 10-40 parts of DHA algae oil, 5-15 parts of protein, frozen Dry protection agent 40-70 parts, stabilizer 0.1-5 parts, emulsifier 0.1-5 parts, flavoring agent 0.05-0.2 parts, purified water 285-667 parts.

Further, 1-10 parts by weight of a filler is also included.

Further, 0.1-0.5 parts by weight of essence is also included.

Further, the protein is selected from at least one of milk protein, whey protein isolate, whey protein concentrate, soybean protein and zein;

The lyoprotectant is selected from sugars and/or sugar alcohols;

The emulsifier is selected from at least one of milk lecithin, soybean lecithin, lecithin, microcrystalline cellulose, glycerol monostearate, medium chain triglyceride, sucrose fatty acid ester, Span 60 and sodium caseinate kind;

The flavoring agent is selected from at least one of citric acid, malic acid, sucralose, steviol glycosides, aspartame and cyclamate;

The filler is at least one selected from maltodextrin, inulin, potato starch, sweet potato starch, resistant starch and resistant dextrin.

Further, the carbohydrate is selected from at least one of L-arabinose, fructooligosaccharide, polydextrose, galactooligosaccharide, stachyose, xylooligosaccharide, lactose, trehalose and glucose;

The sugar alcohols are selected from at least one of D-mannitol, isomalt and xylitol;

The weight ratio of the sugar alcohols to sugars is (1-3):1.

Further, the fragrance is selected from sweet orange essence and/or passion fruit essence.

A preparation method of nanoscale DHA algae oil freeze-dried instant tablets, comprising the steps of:

S1: Divide the purified water into two parts, the mass ratio of the first part of purified water to the second part of purified water is (1-2): 1, and the DHA algae oil, protein, emulsifier and the first part of purified water are dispersed at high speed The homogenizer mixes evenly;

S2: Mix the lyoprotectant, stabilizer, flavoring agent, filler and the second part of purified water evenly, and heat and hydrate in a water bath at 60-85°C for 10-120min;

S3: Mix the mixed solution prepared in step S1, the mixed solution prepared in step S2, and the essence through a high-speed dispersing homogenizer to obtain an emulsion; then transfer the emulsion to a high-pressure homogenizer for homogenization treatment 2-3 times, Obtain nano-emulsion;

S4: Transfer the nano-emulsion prepared in step S3 into the mold, then send it into the drying chamber, pre-freeze at -40°C for 3 hours, and the vacuum degree is 0Pa; keep the vacuum degree at 0Pa and turn on the heating system, sublimation and drying, to Heat up at a rate of 1°C per minute. When the temperature reaches -32°C, keep warm for 2 hours; when the temperature reaches -25°C, keep warm for 4 hours; when the temperature reaches -20°C, keep warm for 6 hours; when the temperature reaches -10°C , keep warm for 4h; when the temperature reaches -0°C, keep warm for 2.5h; when the temperature reaches 10°C, keep warm for 1.5h; then turn on the vacuum system, when the temperature reaches 30°C and the vacuum degree is 20Pa, keep warm for 1.5h, that is, Freeze-dried instant tablets.

Preferably, the mass ratio of the first purified water to the second purified water is 1.5:1.

Further, in step S1, the conditions for homogenizing by a high-speed dispersing homogenizer are: the rotation speed is 8000-15000 r/min, and the homogenization takes 2-5 minutes.

Further, in step S3, the conditions for homogenization by a high-speed dispersing homogenizer are: the rotating speed is 8000-15000r/min, and the time is 1-2min;

The conditions for homogenization by a high-pressure homogenizer are: the homogenization pressure is 500-1200 bar.

Further, in step S3, the mixed liquid prepared in step S1, the mixed liquid prepared in step S2 and the essence are mixed by a high-speed dispersing homogenizer to obtain an emulsion.

In summary, the present invention has the following beneficial effects:

1. In this application, by adding protein, since the protein can form a barrier at the oil-water interface, DHA algae oil will be wrapped after freeze-drying, thereby improving the fishy smell brought by DHA algae oil; by adding a freeze-drying protective agent, the sample can be preserved during the freeze-drying process. A large number of tiny ice crystals are produced, so that the freeze-dried instant tablets can better maintain the particle size of the original emulsion after rehydration, and improve the absorption rate and bioavailability; by reasonably matching the proportion of freeze-drying protective agents and the distribution ratio of each component, it is easier to dry A loose and porous structure is formed, and the instant taste is better.

2. The preparation method of the present application can form a more regular skeleton structure through the freeze-dried flakes produced by homogeneous treatment, and the algae oil particles can reach the nanometer level and are evenly distributed, with good instant solubility, good taste, and convenient consumption, because it is Nano-scale, so it is better absorbed; the water-soluble components and fat-soluble components are dissolved and mixed separately, so that the materials are fully mixed and evenly mixed in their respective dispersion systems. After homogenization, the product adopts vacuum freeze-drying technology to minimize the DHA in the processing process Algae oil is oxidized to retain higher activity, which is convenient for storage and portability.

Description of drawings

In order to more clearly illustrate the technical solutions in the embodiments of the present invention or the prior art, the following will briefly introduce the drawings that need to be used in the description of the embodiments or the prior art. Obviously, the accompanying drawings in the following description These are some embodiments of the present invention. For those skilled in the art, other drawings can also be obtained according to these drawings without any creative effort.

Fig. 1 is the particle size distribution figure after rehydration of the freeze-dried instant tablet of Example 1 of the present application;

Fig. 2 is the particle size distribution figure of comparative example 3 freeze-dried liquid of the present application;

Fig. 3 is the particle size distribution diagram of the lyophilized instant tablet of Comparative Example 5 of the present application after rehydration.

Detailed ways

In order to make the purpose, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions in the embodiments of the present invention will be clearly and completely described below in conjunction with the accompanying drawings 1-3 in the embodiments of the present invention. Obviously, the described The embodiments are some of the embodiments of the present invention, but not all of them. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without creative efforts fall within the protection scope of the present invention.

All raw materials used in this application can be obtained commercially.

Example

Example 1

The preparation method of nanoscale DHA algae oil freeze-dried instant tablet comprises the following steps:

S1: Divide the purified water into two parts, mix 20 parts of DHA algae oil, 10 parts of protein, 6.3 parts of emulsifier and 300 parts of the first purified water through a high-speed dispersing homogenizer at a speed of 10000r/min, mix for 3 minutes, and set aside;

Wherein, the protein is whey protein isolate;

The emulsifier is composed of 5 parts of milk lecithin, 0.8 part of medium chain triglyceride and 0.5 part of soybean lecithin;

S2: Add 58.4 parts of lyoprotectant, 0.2 parts of stabilizer, 0.1 part of flavoring agent and 5 parts of filler to the second part of 200 parts of purified water, heat and hydrate in a water bath at 70°C for 60 minutes until completely dissolved, and set aside;

Among them, the lyoprotectant consists of 38.4 parts of D-mannitol and 20 parts of lactose;

The stabilizer is xanthan gum;

The flavoring agent is sucralose;

The filler is inulin;

S3: Mix the oil dispersion prepared in step S1 and the water dispersion prepared in step S2 uniformly through a high-speed dispersing homogenizer at a speed of 10000 r/min for 2 minutes to obtain an oil-in-water emulsion;

Then the emulsion is transferred to a high-pressure homogenizer, and the homogenization pressure is 1000 bar, and the homogenization is performed 3 times to obtain a nanoscale emulsion;

S4: Transfer the nano-emulsion prepared in step S3 into the mold, and then send it into the drying bin for pre-freezing. The pre-freezing temperature is -40°C, the pre-freezing time is 3h, the temperature control time is 50min, and the pre-freezing vacuum degree is 0 Pa;

After pre-freezing, keep the vacuum at 0 Pa at -40°C for 3 hours, turn on the heating system, raise the temperature to -32°C at a rate of 1°C per minute, and then keep warm for 2 hours; The heating rate is raised to -25°C, and then kept for 4 hours, then raised to -20°C at a heating rate of 1°C per minute, then kept at a temperature of 6 hours, and then raised to -10°C at a heating rate of 1°C per minute, Then keep it warm for 4 hours, then raise the temperature to 0°C at a rate of 1°C per minute, and then keep it for 2.5 hours; then raise the temperature to 10°C at a rate of 1°C per minute, and then keep it for 1.5 hours;

Then turn on the vacuum system, raise the vacuum degree to 20 Pa, then raise the temperature to 30° C. at a rate of 1° C. per minute, and then keep it warm for 1.5 hours to complete the drying to obtain freeze-dried instant tablets.

Example 2

The only difference with Example 1 is that the weight portion of DHA algae oil is 15 parts.

Example 3

The only difference with Example 1 is that the weight portion of DHA algae oil is 25 parts.

Example 4

The only difference with Example 1 is that the weight portion of DHA algae oil is 30 parts.

Example 5

The only difference from Example 1 is that the first part of purified water has 240 parts by weight, and the second part of purified water has 160 parts by weight.

Example 6

The only difference from Example 1 is that the first part of purified water has 200 parts by weight, and the second part of purified water has 135 parts by weight.

Example 7

The only difference from Example 1 is that the first part of purified water has 185 parts by weight, and the second part of purified water has 100 parts by weight.

Example 8

The only difference from Example 1 is that the homogeneous pressure in step S3 is 800 bar.

Example 9

The only difference from Example 1 is that the homogeneous pressure in step S3 is 1200 bar.

Example 10

The only difference from Example 1 is that the weight part of the lyoprotectant D-mannitol is 43.4 parts, and the weight part of lactose is 15 parts.

Example 11

The only difference from Example 1 is that the weight part of the lyoprotectant D-mannitol is 30 parts, and the weight part of lactose is 28.4 parts.

Example 12

The difference from Example 1 is that the oil dispersion prepared in step S1, the water dispersion prepared in step S2, and 0.1 part of sweet orange essence are mixed uniformly by a high-speed dispersing homogenizer at a speed of 10,000 r/min for 2 minutes , to obtain an oil-in-water emulsion.

Example 13

The only difference with Example 1 is that the protein is 10 parts of milk protein.

Example 14

The only difference from Example 1 is that the emulsifier is 6.3 parts, consisting of 5 parts of soybean lecithin, 0.8 part of sucrose fatty acid ester and 0.5 part of microcrystalline cellulose.

Example 15

The only difference from Example 1 is that the lyoprotectant is 38.4 parts of xylitol and 20 parts of glucose.

comparative example

Comparative example 1

The only difference with Example 1 is that the weight portion of DHA algae oil is 35 parts.

Comparative example 2

The weight part of the first purified water is 400 parts, and the weight part of the second purified water is 267 parts.

Comparative example 3

The only difference from Embodiment 1 is that the operation of step S4 is not performed.

Comparative example 4

The only difference from Example 1 is that the weight part of the lyoprotectant D-mannitol is 26.6 parts, and the weight part of lactose is 13.4 parts.

Comparative example 5

The preparation method of nanoscale DHA algae oil freeze-dried instant tablet comprises the following steps:

S1: Add 58.4 parts of lyoprotectant, 0.2 parts of stabilizer, 0.1 part of flavoring agent and 5 parts of filler to 500 parts of purified water, heat and hydrate in a water bath at 70°C for 60 minutes until completely dissolved;

Among them, the lyoprotectant consists of 38.4 parts of D-mannitol and 20 parts of lactose;

The stabilizer is xanthan gum;

The flavoring agent is sucralose;

The filler is inulin;

S2: Add 20 parts of DHA algae oil, 10 parts of protein, and 6.3 parts of emulsifier to the mixed solution prepared in step S1, and mix for 3 minutes through a high-speed dispersing homogenizer at a speed of 10000 r/min, and set aside;

Wherein, the protein is whey protein isolate;

The emulsifier is composed of 5 parts of milk lecithin, 0.8 part of medium chain triglyceride and 0.5 part of soybean lecithin;

S3: Mix the mixed solution prepared in step S2 uniformly with a high-speed dispersing homogenizer at a speed of 10000r/min for 2min to obtain an emulsion;

Then the emulsion is transferred to a high-pressure homogenizer, and the homogenization pressure is 1000 bar, and the homogenization is performed 3 times to obtain a nanoscale emulsion;

S4: Same as Example 1.

Comparative example 6

The only difference from Example 1 is that no protein is added.

performance test

1. Determination of embedding rate

The freeze-dried instant tablet prepared by embodiment 1-4 and comparative example 1 is tested as follows:

Test method: Take 2g of the whole freeze-dried instant tablet sample in a beaker, add 20mL of n-hexane, take out the instant tablet sample and filter it after fully shaking, then rinse the beaker with 20mL of n-hexane and combine the filtrate, put it into the 1/10,000th balance to weigh In a heavy beaker, volatilize the n-hexane in an 80°C water bath, wipe off the water in the beaker, and then weigh and record the surface oil content with a ten-thousandth balance. The test results are shown in Table 1.

Embedding rate=1-(DHA content on the surface of the freeze-dried instant tablet/the total amount of DHA in the freeze-dried instant tablet)

The embedding rate of table 1 embodiment 1-4 and comparative example 1 freeze-dried instant tablet

Test items Example 1 Example 2 Example 3 Example 4 Comparative example 1 Amount added (wt%) 20 15 25 30 35 Surface oil content (mg) 37.3 35.2 44.6 53.9 65.4 Total (mg) 400 300 500 600 700 Embedding rate (%) 90.68 88.27 91.08 91.09 90.66

In conjunction with Examples 1-4 and Comparative Example 1 and in conjunction with Table 1, it can be seen that the embedment rate of the freeze-dried instant tablet prepared by the method of the present application is about 90%, and the embedment rate also increases with the increase of the amount of DHA algae oil added. It will increase, but when it reaches a certain value, the excess oil cannot be wrapped due to the saturation of the wall material, resulting in a decrease in the embedding rate. That is, the amount of DHA added in the sample of the freeze-dried instant tablet is 20-30%, and within the range of the added amount, the DHA algae oil can be better embedded in the freeze-dried instant tablet.

2. Simulated oral dissolution experiment

The freeze-dried instant tablet prepared by Example 1, 5-11, 15 and Comparative Example 2, 4 is respectively tested as follows:

Test method: Put a complete freeze-dried instant tablet into a beaker placed in a constant temperature oscillating water bath at 37°C with an oscillation frequency of 50r/min. The beaker contains 5mL preheated simulated oral digestive juice (5mg/mL mucin , 150U/mL salivary amylase, pH6.8), put into the freeze-dried instant tablet and start timing, when the freeze-dried tablet is completely dissolved, the timing ends, and the test results are shown in Table 3. The concentration of the lyophilized liquid is the ratio of the weight (g) of all ingredients of the instant tablet to the volume of water (mL).

Table 2 embodiment 1,5-7 and comparative example 2 freeze-dried instant tablet dissolving time

Test items Concentration of lyophilized liquid (g/100mL) Dissolving time (s) Example 1 20 4.52 Example 5 25 5.08 Example 6 30 5.61 Example 7 35 6.12 Example 8 — 4.55 Example 9 — 4.61 Example 10 — 5.12 Example 11 — 4.43 Example 15 — 5.19 Comparative example 2 15 4.45 Comparative example 4 — 8.55

In combination with Examples 1, 5-7 and Comparative Example 2 and in combination with Table 2, it can be seen that in the simulated oral cavity experiment, all the freeze-dried instant tablets of the examples can be dissolved quickly, and the lower the concentration of the lyophilized solution, the faster the dissolution rate. The actual speed in the oral cavity may be faster. This is because the lyophilized liquid with lower concentration can still maintain the original skeleton structure after drying. After contacting with water, the capillary force absorbs water faster and dissolves quickly. The dissolution time of 15g/100mL is close to that of 20g/100mL. It is possible that the water diffusion rate of the instant tablet has reached saturation under the same volume, and further reduction of the concentration will not significantly increase the dissolution rate. That is, it is more appropriate to set the concentration of the lyophilized liquid at 20-30g/100mL. Taking advantage of the advantage of rapid dissolution, it is more convenient and quicker to supplement DHA algae oil in the form of freeze-dried instant tablets.

Combining Examples 1, 8 and 9 and Table 2, it can be seen that pressure changes have little effect on instant solubility.

Combining Examples 1, 10, 11 and 15 and Comparative Example 4 with Table 2, it can be seen that when the proportion of lactose increases, the dissolution time is short, but the high content of lactose in actual production will cause the finished product to collapse, and the freeze-dried skeleton is not solid , the content of the lyoprotectant that provides the skeleton support should be appropriately increased. After changing the type of lyoprotectant, because the instant tablet added with xylitol and glucose has a slight collapse, the product shows that a dense sugar layer is formed, resulting in a significantly slower dissolution rate. When the proportion of lyoprotectant is insufficient, the dissolution time is very long, and the relative proportion of protein increases, resulting in soft finished product, lack of hard skeleton, and affecting instant solubility.

3. Particle size test

The freeze-dried instant tablet prepared by embodiment 1, 8-11 and comparative example 3-5 is tested as follows:

Test method: Take 1 g of freeze-dried instant tablet and rehydrate it in 20 mL of purified water. After mixing thoroughly, use Zetasizer Nano ZS90 (Malvern, UK) to measure the particle size and polydispersity coefficient of the sample. sample. Each sample was carried out in parallel three times, and the average value was taken. The test results are shown in Table 3, Table 4, Fig. 1, Fig. 2, Fig. 3.

Table 3 Embodiment 1, 8 and 9 freeze-dried instant tablet particle size and pressure

Test items Example 1 Example 8 Example 9 Homogeneous pressure (bar) 1000 800 1200 Particle size (nm) 250.4 269.3 232.5

In conjunction with Examples 1, 8 and 9 and in conjunction with Table 3, it can be seen that the greater the pressure of the freeze-dried solution, the smaller the particle size of the freeze-dried instant tablet, and the high-pressure homogenization belongs to the high-energy emulsification process. The primary particles in the internal phase are broken into nano-droplets. Combined with Table 2, it can be judged that the particle size after rehydration is not directly related to the dissolution rate.

Table 4 embodiment 1,10,11 and 15 and comparative example 3-5 lyophilized instant tablet particle diameter and polydispersity coefficient

Test items Example 1 Example 10 Example 11 Example 15 Comparative example 3 Comparative example 4 Comparative example 5 Particle size (nm) 250.4 255.6 247.6 271.5 235.6 355.6 422.4 polydispersity coefficient 0.237 0.241 0.232 0.258 0.263 0.362 0.424

Fig. 1 is the particle size distribution figure after the rehydration of the freeze-dried instant tablet of embodiment 1;

Figure 2 is a particle size distribution diagram of the freeze-dried liquid of Comparative Example 3.

Figure 3 is a particle size distribution diagram of the freeze-dried instant tablet of Comparative Example 5 after rehydration.

In combination with Examples 1, 10, 11 and 15 and in conjunction with Table 4, it can be seen that the particle size of the freeze-dried instant tablet is about 250nm after rehydration, and changing the concentration of the freeze-drying protective agent has little effect on the rehydration particle size, and the polydispersity coefficient (PDI) is close, and changing the type of lyoprotectant directly affects the rehydration particle size, and the PDI increases, indicating that the combination of D-mannitol and lactose is better than the combination of xylitol and glucose, because the former is more effective in the freeze-drying process. It is best to maintain the skeleton structure of the lyophilized liquid to prevent collapse and ensure that the droplets will not aggregate and cause particle size to increase.

In conjunction with Example 1 and Comparative Examples 3 and 4 and Figures 1-2 and in conjunction with Table 4, it can be seen that after adding the lyoprotectant, the rehydration of the lyophilized instant tablet can better maintain the particle size of the original lyophilized liquid, The particle size of the emulsion will increase slightly after drying, and when the concentration of the added lyoprotectant is too low, the particle size will increase significantly after rehydration.

In combination with Example 1 and Comparative Example 5 and Fig. 3 and in combination with Table 4, it can be seen that each system is not uniformly dispersed during the treatment of the lyophilized liquid, which directly leads to an increase in the particle size of the lyophilized rehydration, and the polydispersity coefficient of the emulsion is 0.424, indicating that The molecular weight distribution of the mixed system is wide, and the emulsion is not uniform.

4. Sensory evaluation

The freeze-dried instant tablet prepared by embodiment 1, 12-15 and comparative example 6 is tested as follows:

Fishy smell test method: A sensory evaluation team composed of 15 people who have undergone certain training conducts sensory evaluation. The quality attribute tested was fishy odour. All samples were presented to each panelist simultaneously in labeled and covered glass containers. Use 9-point scoring method to score according to the severity of fishy smell, and the score is an integer between 1-9, wherein 1=dislikes very much (severe fishy smell), 9=likes very much (light fishy smell or no fishy smell) ), the sensory scoring results are shown in Table 5.

Table 5 Example 1, 12-15 and comparative example 6 freeze-dried instant tablet sensory evaluation

Test items Example 1 Example 12 Example 13 Example 14 Example 15 Comparative example 6 fishy smell 7.5 8 7.8 7.4 7.4 4.3

Combining Example 1, 12-15 and Comparative Example 6 with Table 5, it can be seen that not adding protein will significantly reduce the score of fishy smell, and changing the type of protein will also affect the score of fishy smell, which may be due to different protein It may have different flavors, so it has different wrapping and masking effects on fishy smell. In addition, changing the types of lyoprotectant and emulsifier had little effect on fishy smell. Adding essence can also improve the fishy smell brought by DHA algae oil and improve the acceptable level of entrance.

Combining Examples 1, 5-7 and Comparative Example 2 with Table 2 and Table 5, it can be seen that the lower the concentration of the lyophilized solution, the higher the sensory score and the better the instant solubility.

Finally, it should be noted that: the above embodiments are only used to illustrate the technical solutions of the present invention, rather than limiting them; although the present invention has been described in detail with reference to the foregoing embodiments, those of ordinary skill in the art should understand that: It is still possible to modify the technical solutions described in the foregoing embodiments, or perform equivalent replacements for some or all of the technical features; and these modifications or replacements do not make the essence of the corresponding technical solutions deviate from the technical solutions of the various embodiments of the present invention. scope.

Claims (10)

1. The nanoscale DHA algae oil freeze-dried instant tablet is characterized by comprising the following raw materials in parts by weight: 10-40 parts of DHA algae oil, 5-15 parts of protein, 40-70 parts of freeze-drying protective agent, 0.1-5 parts of stabilizing agent, 0.1-5 parts of emulsifying agent, 0.05-0.2 part of flavoring agent and 285-667 parts of purified water.

2. The nanoscale DHA algae oil freeze-dried instant tablet according to claim 1, further comprising 1-10 parts by weight of bulking agent selected from at least one of maltodextrin, inulin, potato starch, sweet potato starch, resistant starch, and resistant dextrin.

3. The nanoscale DHA algae oil freeze-dried instant tablet according to claim 1 or 2, further comprising 0.1-0.5 weight parts of essence.

4. The nanoscale DHA algae oil freeze-dried instant tablet of claim 1 wherein the protein is selected from at least one of milk protein, isolated whey protein, concentrated whey protein, soy protein, and zein;

the lyoprotectant is selected from saccharides and/or sugar alcohols;

the emulsifier is at least one selected from milk phospholipid, soybean phospholipid, lecithin, microcrystalline cellulose, glyceryl monostearate, medium chain triglyceride, sucrose fatty acid ester, span 60 and sodium caseinate;

the flavoring agent is at least one selected from citric acid, malic acid, sucralose, stevioside, aspartame and cyclamate.

5. The nanoscale DHA algae oil freeze-dried instant tablet according to claim 4, wherein the saccharide is selected from at least one of L-arabinose, fructo-oligosaccharide, polydextrose, galacto-oligosaccharide, stachyose, xylo-oligosaccharide, lactose, trehalose, and glucose;

the sugar alcohol is at least one selected from D-mannitol, isomalt and xylitol;

the weight ratio of sugar alcohols to sugar is (1-3): 1.

6. a nanoscale DHA algae oil freeze-dried instant tablet according to claim 3, wherein the flavour is selected from orange flavour and/or passion fruit flavour.

7. The method for preparing the nanoscale DHA algae oil freeze-dried instant tablet according to any one of claims 1 to 6, which is characterized by comprising the following steps:

s1: dividing the purified water into two parts, wherein the mass ratio of the first part of purified water to the second part of purified water is (1-2): 1, uniformly mixing DHA algae oil, protein, an emulsifying agent and a first part of purified water through a high-speed dispersion homogenizer;

s2: uniformly mixing the freeze-drying protective agent, the stabilizing agent, the flavoring agent, the filling agent and the second part of purified water, and heating and hydrating in a water bath at 60-85 ℃ for 10-120min;

s3: uniformly mixing the mixed solution prepared in the step S1 and the mixed solution prepared in the step S2 with essence by a high-speed dispersion homogenizer to obtain emulsion; then transferring the emulsion to a high-pressure homogenizer for homogenization treatment for 2-3 times to obtain nano-scale emulsion;

s4: transferring the nano-scale emulsion prepared in the step S3 into a mold, then sending into a drying bin, pre-freezing for 3 hours at the temperature of minus 40 ℃ and the vacuum degree of 0Pa; starting a heating system with the vacuum degree of 0Pa, performing sublimation drying, heating at a heating rate of 1 ℃ per minute, and preserving heat for 2 hours when the temperature reaches-32 ℃; when the temperature reaches-25 ℃, preserving the heat for 4 hours; when the temperature reaches-20 ℃, preserving the heat for 6 hours; when the temperature reaches-10 ℃, preserving the heat for 4 hours; when the temperature reaches-0 ℃, preserving the heat for 2.5h; when the temperature reaches 10 ℃, preserving the heat for 1.5 hours; and then starting a vacuum system, and when the temperature reaches 30 ℃ and the vacuum degree is 20Pa, preserving the heat for 1.5 hours to obtain the freeze-dried instant tablet.

8. The method for preparing the nanoscale DHA algae oil freeze-dried instant tablet according to claim 7, wherein in step S1, the conditions for homogenization by a high-speed dispersion homogenizer are: the rotating speed is 8000-15000r/min, and the homogenization is 2-5min.

9. The method for preparing a nanoscale DHA algae oil freeze-dried instant tablet according to claim 7, wherein in step S3, the conditions for homogenization by a high-speed dispersion homogenizer are: the rotating speed is 8000-15000r/min, and the time is 1-2min;

the conditions for homogenization by the high-pressure homogenizer are: the homogenizing pressure is 500-1200bar.

10. The method for preparing the nanoscale DHA algae oil freeze-dried instant tablet according to claim 7, wherein in the step S3, the mixed solution prepared in the step S1, the mixed solution prepared in the step S2 and the essence are mixed by a high-speed dispersion homogenizer to obtain an emulsion.